LOCUS       DAZAJZ010000001        87071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000001 DAZAJZ010000000
VERSION     DAZAJZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.001"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..891)
                     /locus_tag="ACG86D_000001"
     CDS             complement(<1..891)
                     /locus_tag="ACG86D_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3139187.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKY"
     gene            1074..2198
                     /locus_tag="ACG86D_000002"
     CDS             1074..2198
                     /locus_tag="ACG86D_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3139188.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2425..3342
                     /locus_tag="ACG86D_000003"
     CDS             2425..3342
                     /locus_tag="ACG86D_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3139189.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3335..4168
                     /locus_tag="ACG86D_000004"
     CDS             3335..4168
                     /locus_tag="ACG86D_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3139190.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4251..5369
                     /locus_tag="ACG86D_000005"
     CDS             4251..5369
                     /locus_tag="ACG86D_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3139191.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5390..7261
                     /locus_tag="ACG86D_000006"
     CDS             5390..7261
                     /locus_tag="ACG86D_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN3139192.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            7255..8478
                     /locus_tag="ACG86D_000007"
     CDS             7255..8478
                     /locus_tag="ACG86D_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3139193.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(8564..10498)
                     /locus_tag="ACG86D_000008"
     CDS             complement(8564..10498)
                     /locus_tag="ACG86D_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3139194.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            10724..11380
                     /locus_tag="ACG86D_000009"
     CDS             10724..11380
                     /locus_tag="ACG86D_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN3139195.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(11454..11807)
                     /locus_tag="ACG86D_000010"
     CDS             complement(11454..11807)
                     /locus_tag="ACG86D_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3139196.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            11999..12928
                     /locus_tag="ACG86D_000011"
     CDS             11999..12928
                     /locus_tag="ACG86D_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN3139197.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            12939..13775
                     /locus_tag="ACG86D_000012"
     CDS             12939..13775
                     /locus_tag="ACG86D_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN3139198.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            14023..14799
                     /locus_tag="ACG86D_000013"
     CDS             14023..14799
                     /locus_tag="ACG86D_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3139199.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            14975..15718
                     /locus_tag="ACG86D_000014"
     CDS             14975..15718
                     /locus_tag="ACG86D_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139200.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            15711..17291
                     /locus_tag="ACG86D_000015"
     CDS             15711..17291
                     /locus_tag="ACG86D_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN3139201.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(17542..18021)
                     /locus_tag="ACG86D_000016"
     CDS             complement(17542..18021)
                     /locus_tag="ACG86D_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN3139202.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18037..18789)
                     /locus_tag="ACG86D_000017"
     CDS             complement(18037..18789)
                     /locus_tag="ACG86D_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN3139203.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18906..18976
                     /locus_tag="ACG86D_000018"
     tRNA            18906..18976
                     /locus_tag="ACG86D_000018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18938..18940,aa:Gly,seq:ccc)
     gene            19337..20062
                     /locus_tag="ACG86D_000019"
     CDS             19337..20062
                     /locus_tag="ACG86D_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN3139204.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            20317..20583
                     /locus_tag="ACG86D_000020"
     CDS             20317..20583
                     /locus_tag="ACG86D_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN3139205.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            20580..21011
                     /gene="ruvX"
                     /locus_tag="ACG86D_000021"
     CDS             20580..21011
                     /gene="ruvX"
                     /locus_tag="ACG86D_000021"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN3139206.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            21036..21341
                     /locus_tag="ACG86D_000022"
     CDS             21036..21341
                     /locus_tag="ACG86D_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN3139207.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21422..22567)
                     /locus_tag="ACG86D_000023"
     CDS             complement(21422..22567)
                     /locus_tag="ACG86D_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3139208.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22628..23275)
                     /locus_tag="ACG86D_000024"
     CDS             complement(22628..23275)
                     /locus_tag="ACG86D_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3139209.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            23469..23762
                     /locus_tag="ACG86D_000025"
     CDS             23469..23762
                     /locus_tag="ACG86D_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139210.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            23806..24117
                     /locus_tag="ACG86D_000026"
     CDS             23806..24117
                     /locus_tag="ACG86D_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139211.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            24114..24738
                     /locus_tag="ACG86D_000027"
                     /pseudo
     CDS             24114..24738
                     /locus_tag="ACG86D_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24879..25037
                     /locus_tag="ACG86D_000028"
     CDS             24879..25037
                     /locus_tag="ACG86D_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139212.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            25038..25400
                     /locus_tag="ACG86D_000029"
     CDS             25038..25400
                     /locus_tag="ACG86D_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139213.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            25437..26285
                     /locus_tag="ACG86D_000030"
     CDS             25437..26285
                     /locus_tag="ACG86D_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN3139214.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            26275..27750
                     /locus_tag="ACG86D_000031"
     CDS             26275..27750
                     /locus_tag="ACG86D_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN3139215.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            28016..28423
                     /locus_tag="ACG86D_000032"
     CDS             28016..28423
                     /locus_tag="ACG86D_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN3139216.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            28426..28641
                     /locus_tag="ACG86D_000033"
     CDS             28426..28641
                     /locus_tag="ACG86D_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139217.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            28645..29025
                     /locus_tag="ACG86D_000034"
     CDS             28645..29025
                     /locus_tag="ACG86D_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3139218.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            29159..29314
                     /locus_tag="ACG86D_000035"
     CDS             29159..29314
                     /locus_tag="ACG86D_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN3139219.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            29383..29856
                     /locus_tag="ACG86D_000036"
     CDS             29383..29856
                     /locus_tag="ACG86D_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN3139220.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            29853..31547
                     /locus_tag="ACG86D_000037"
     CDS             29853..31547
                     /locus_tag="ACG86D_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3139221.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            31513..31698
                     /locus_tag="ACG86D_000038"
     CDS             31513..31698
                     /locus_tag="ACG86D_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139222.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            31702..32877
                     /locus_tag="ACG86D_000039"
     CDS             31702..32877
                     /locus_tag="ACG86D_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3139223.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            32870..34393
                     /locus_tag="ACG86D_000040"
     CDS             32870..34393
                     /locus_tag="ACG86D_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3139224.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            34449..34733
                     /locus_tag="ACG86D_000041"
     CDS             34449..34733
                     /locus_tag="ACG86D_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3139225.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            34720..35055
                     /locus_tag="ACG86D_000042"
     CDS             34720..35055
                     /locus_tag="ACG86D_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3139226.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            35350..35976
                     /locus_tag="ACG86D_000043"
     CDS             35350..35976
                     /locus_tag="ACG86D_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139227.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            36718..37554
                     /locus_tag="ACG86D_000044"
     CDS             36718..37554
                     /locus_tag="ACG86D_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN3139228.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            37618..38049
                     /locus_tag="ACG86D_000045"
     CDS             37618..38049
                     /locus_tag="ACG86D_000045"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139229.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(38111..39433)
                     /locus_tag="ACG86D_000046"
     CDS             complement(38111..39433)
                     /locus_tag="ACG86D_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN3139230.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(39587..41227)
                     /locus_tag="ACG86D_000047"
     CDS             complement(39587..41227)
                     /locus_tag="ACG86D_000047"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN3139231.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            41560..42030
                     /locus_tag="ACG86D_000048"
     CDS             41560..42030
                     /locus_tag="ACG86D_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN3139232.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            42252..43355
                     /locus_tag="ACG86D_000049"
     CDS             42252..43355
                     /locus_tag="ACG86D_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN3139233.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            43639..44319
                     /locus_tag="ACG86D_000050"
     CDS             43639..44319
                     /locus_tag="ACG86D_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3139234.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            44409..45443
                     /locus_tag="ACG86D_000051"
     CDS             44409..45443
                     /locus_tag="ACG86D_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN3139235.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            45457..45792
                     /locus_tag="ACG86D_000052"
     CDS             45457..45792
                     /locus_tag="ACG86D_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139236.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            45776..48256
                     /locus_tag="ACG86D_000053"
     CDS             45776..48256
                     /locus_tag="ACG86D_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139237.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            48312..49871
                     /locus_tag="ACG86D_000054"
     CDS             48312..49871
                     /locus_tag="ACG86D_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139238.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            49868..51232
                     /locus_tag="ACG86D_000055"
     CDS             49868..51232
                     /locus_tag="ACG86D_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3139239.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            51403..52473
                     /locus_tag="ACG86D_000056"
     CDS             51403..52473
                     /locus_tag="ACG86D_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139240.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            52488..53156
                     /locus_tag="ACG86D_000057"
     CDS             52488..53156
                     /locus_tag="ACG86D_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139241.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            53176..53754
                     /locus_tag="ACG86D_000058"
     CDS             53176..53754
                     /locus_tag="ACG86D_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3139242.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            54155..55066
                     /locus_tag="ACG86D_000059"
     CDS             54155..55066
                     /locus_tag="ACG86D_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3139243.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(55444..56361)
                     /gene="rnhC"
                     /locus_tag="ACG86D_000060"
     CDS             complement(55444..56361)
                     /gene="rnhC"
                     /locus_tag="ACG86D_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN3139244.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            56563..57000
                     /gene="zapA"
                     /locus_tag="ACG86D_000061"
     CDS             56563..57000
                     /gene="zapA"
                     /locus_tag="ACG86D_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN3139245.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            57058..57606
                     /locus_tag="ACG86D_000062"
     CDS             57058..57606
                     /locus_tag="ACG86D_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFN3139246.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            57721..60081
                     /locus_tag="ACG86D_000063"
     CDS             57721..60081
                     /locus_tag="ACG86D_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN3139247.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            60168..60482
                     /gene="trxA"
                     /locus_tag="ACG86D_000064"
     CDS             60168..60482
                     /gene="trxA"
                     /locus_tag="ACG86D_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN3139248.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            60664..62442
                     /gene="uvrC"
                     /locus_tag="ACG86D_000065"
     CDS             60664..62442
                     /gene="uvrC"
                     /locus_tag="ACG86D_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN3139249.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            62587..63486
                     /locus_tag="ACG86D_000066"
     CDS             62587..63486
                     /locus_tag="ACG86D_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139250.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            63479..64741
                     /locus_tag="ACG86D_000067"
     CDS             63479..64741
                     /locus_tag="ACG86D_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139251.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            64902..66800
                     /locus_tag="ACG86D_000068"
     CDS             64902..66800
                     /locus_tag="ACG86D_000068"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN3139252.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(67130..67849)
                     /locus_tag="ACG86D_000069"
     CDS             complement(67130..67849)
                     /locus_tag="ACG86D_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN3139253.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    68039..68086
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            68186..68518
                     /locus_tag="ACG86D_000070"
     CDS             68186..68518
                     /locus_tag="ACG86D_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139254.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            68505..70508
                     /locus_tag="ACG86D_000071"
     CDS             68505..70508
                     /locus_tag="ACG86D_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN3139255.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            70527..70997
                     /locus_tag="ACG86D_000072"
     CDS             70527..70997
                     /locus_tag="ACG86D_000072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN3139256.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            71018..71602
                     /locus_tag="ACG86D_000073"
     CDS             71018..71602
                     /locus_tag="ACG86D_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFN3139257.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            71617..72603
                     /locus_tag="ACG86D_000074"
     CDS             71617..72603
                     /locus_tag="ACG86D_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN3139258.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            72593..72904
                     /locus_tag="ACG86D_000075"
     CDS             72593..72904
                     /locus_tag="ACG86D_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN3139259.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            72926..74707
                     /locus_tag="ACG86D_000076"
     CDS             72926..74707
                     /locus_tag="ACG86D_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN3139260.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            74700..76076
                     /locus_tag="ACG86D_000077"
     CDS             74700..76076
                     /locus_tag="ACG86D_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN3139261.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            76083..76718
                     /locus_tag="ACG86D_000078"
     CDS             76083..76718
                     /locus_tag="ACG86D_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN3139262.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            76748..78103
                     /locus_tag="ACG86D_000079"
     CDS             76748..78103
                     /locus_tag="ACG86D_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN3139263.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(78295..79689)
                     /locus_tag="ACG86D_000080"
     CDS             complement(78295..79689)
                     /locus_tag="ACG86D_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN3139264.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            79781..80752
                     /locus_tag="ACG86D_000081"
     CDS             79781..80752
                     /locus_tag="ACG86D_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN3139265.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            80749..81531
                     /locus_tag="ACG86D_000082"
     CDS             80749..81531
                     /locus_tag="ACG86D_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN3139266.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(81700..82071)
                     /locus_tag="ACG86D_000083"
     CDS             complement(81700..82071)
                     /locus_tag="ACG86D_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3139267.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            82205..82648
                     /locus_tag="ACG86D_000084"
     CDS             82205..82648
                     /locus_tag="ACG86D_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN3139268.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(82691..83500)
                     /gene="yidA"
                     /locus_tag="ACG86D_000085"
     CDS             complement(82691..83500)
                     /gene="yidA"
                     /locus_tag="ACG86D_000085"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN3139269.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(83519..84886)
                     /locus_tag="ACG86D_000086"
     CDS             complement(83519..84886)
                     /locus_tag="ACG86D_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3139270.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(84870..85700)
                     /locus_tag="ACG86D_000087"
     CDS             complement(84870..85700)
                     /locus_tag="ACG86D_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN3139271.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            85863..86300
                     /locus_tag="ACG86D_000088"
     CDS             85863..86300
                     /locus_tag="ACG86D_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN3139272.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            86376..86996
                     /gene="rpoE"
                     /locus_tag="ACG86D_000089"
     CDS             86376..86996
                     /gene="rpoE"
                     /locus_tag="ACG86D_000089"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN3139273.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        28710 a        14672 c        18016 g        25673 t
ORIGIN      
        1 atactttgaa ggggataacc ctgtatattt tttaaaagct gaagaaaaat gatggatcga
       61 atgaaatcct aatatatctg caatttctgt ataggttaat tcattttcta gcacgagacg
      121 ttttgctttt tccattttta gttctttaaa atagctaata acactttttc cattcacttt
      181 tctaaaatgt ttttttaagc tggctggact gataaaaaaa atttgggcta attgttcaag
      241 caaaaaattt tctgaaagat gttcttctaa atatcgtatg attggatcaa ctaattcatc
      301 tgaataaaga atatgctttt ttttaggaat gactttctca tttgaaggta ttgtcaataa
      361 agcaagttcc tgttgataat gccttggtaa ggaagacaat tgaatttttt gcagacaata
      421 agtaactaat aaggaaagca aatgaatgct catctgttgg catagtatga aaaaagaatt
      481 gatttcctga tcataggaaa tagctggctt actatgtttt agataatcaa ttttgaactg
      541 ttcatttttc agaaaagtaa tgatagatgt tatttcttga tctatagaaa aagattgtcc
      601 cgtaatatgt gctaataatt gctcatcaaa ataaaactgg ataatgataa aagtttgtgt
      661 ttccttttga gtagaaaaga tttggacttc ttgctgttta tcaatgaata aaagggaagg
      721 attatctagt tctatagatt tatgtgcaaa ttcagcatat aaattagcgc tttcatttat
      781 tagtagtcga aaatagggag cagcagataa atgaagatgg gtatctatgg aataggttag
      841 tgtttttttg acaagattgg aggattggtt gtttgttaac aaaagaacca ctttcctttc
      901 tctttttata taaaaagctt tctatttata attaatgact attataacaa aaatgaaaag
      961 ccaaaacgac aagtaaaata tttttttata taaatatttg tgttactaat tccgttaata
     1021 tacataagtg aaagcgtttt atataagaac ttgaaaagaa agaagggata gacatgaaaa
     1081 aaagatacaa attggtgata gctttgctaa acattaccct attattcagc ttaagtgcat
     1141 gtggaaaaac taacgaagaa gtaacagcaa acatcgcaga tccaatgata aaaaaagcca
     1201 ctacattaga agtatatgtc gcaaacggca aactgacaaa ctatgttgaa aatgctgcaa
     1261 aaatctataa ccgcaaaact ggagcaaata ttgacttaaa aattgtgaat gtagcttcgg
     1321 gaacagcaac agtacaaatg atcaccccaa aattggtaac acacgaagat atgccggata
     1381 ttatctccat ggcagacact tcagcagcag gtgtgctgga aaaatttcaa gatgcttttt
     1441 atagtgctga tgaatatgga ttttatgaca aatatggcga tcagttttat gatcaaaaat
     1501 tgaatatttt gcgtcaacaa acgagtaatg aaacgattat tccttgggct agtgatttta
     1561 ccccagcact ttgttattat caaccaaaaa aatttgctga aataggtgta gattttgaag
     1621 aaatcagttc ttgggaagaa tatatccagg tggcaaaagc attaaaagaa aaaactggta
     1681 gttttggaat tgccttgcca gaatctgggg accaagaatt gtttatcaac atgctagccc
     1741 agcagaatca atctttgctt gatgaagaag gaaatatccg tctgaatact aaagaagcga
     1801 aacatgctgc aaagttaatc aaagaaatga tcgactcaga tattgtacat ttctacggtg
     1861 cacaagatgc tgagaaagca tttcaagaat ctgcaatgtt tgttgctggc ggctggtatg
     1921 ctacgaatat gtctttgaat tttcctgatg catctgacaa atggcgcatg gctccactga
     1981 ttcctttttc aaaagaaaat ccaggaaaag gtgcagtatc aggaggatca agtttttatg
     2041 tccctaaaga tgcaaaaaat ccacaagtgg cacaacaatt tcttacattt atgctgacag
     2101 atgaagaatg cttagccaac gcactggagc tgggcgtggc tacatctaac tcattggcct
     2161 atcaaacaga agcagctgag aaaaagtttg actactaagg aggacaaaaa tactatgaac
     2221 tattgaataa aatcaatgaa aatacatcaa atactgtttt accagccact tattcagatg
     2281 ctacgtctta tttgactacc gctgcatata actattggca aacaggtgat tttgaaaatt
     2341 catatctaaa agaagcagaa aattttgctc ataagtacgg cgtaaaatta actggtgatt
     2401 aaaaaatatt aggaggactt aacgatgatg caatcttcgt taccaagcaa gacaataaag
     2461 aacagcaaga aaaaagcaac agatacgtct cataaaaaaa gtgcttatgg attggtcaca
     2521 ccttttgtta ttttctttat tgtgacgatt ttgattccca tcattatctc gtttgctttt
     2581 agttttatga aaattggcta ctcatttgaa tttgttggat ttgacaatta tctagctatc
     2641 ttccgagatc cattgtttat taaatcttac acgaatgttt tgattatcat ggcaggatca
     2701 attccaataa cgatgctatt agctatttac tttgctgttt tattgaattc agcacagtta
     2761 aaaggaaaag gattttttag aactgtctat tatattccaa ccattacatc aacggtggct
     2821 gtagcttccg tattcatgac ttttttcaat ccgactgggt tattcaatgg atttttgaat
     2881 gcgctaggaa ttaacggtgt acagtggtta acaaatcctt tttggatacg aatatcaatg
     2941 atcatcacga tgatttggct tagtgtagga tataacacta ttttattttt agctggtctt
     3001 caagggattt ctaaagagat atacgaaagt gctgaaatag atggagcatc gaaatttcgg
     3061 caattctttt tattaacgat ccccatgcta aagccaatca ttttaatggc cacagtttta
     3121 gccactatca atggcttggg taatttcaat atcccaaata tattctttgg gacttccaat
     3181 ggaccagaaa attcagcttt ggtcgtagga gtcaatttat ataaaacaag ttttgaaatg
     3241 gttgattttg gaaaagcctc tgctattgcc tggacgatgg tgtttgtatc tgttatatta
     3301 tcggctattc aatttaaatt cggaggtgat aaaaatgact aaacaaaaga tacaaagcag
     3361 aatttttacg tacgtcatta ttgtgtttgg tgccttaata tttttgatgc catatttata
     3421 tatgattgta gcttcaacgc aaagtaatca agaaatttta agtggcggaa tcaatttcaa
     3481 gtttggggat atgctattaa aaaactggca agccatcatg gcgagatata attatccctt
     3541 gattatttgg aattcgattg ttattgctgt aatcagtaca gttttagcta cagccagttc
     3601 tacaatggcg ggttatgcat tagcaaagta tcgttttatg gggaataaac tgttgttcaa
     3661 tacagttatg ctatctagga tggtgccttt ttttaccaca ttgattcctt tattttacat
     3721 tatgagtcgt ttaggattat cgaatactta tgcaggtgtg atcatccctt cgattgccag
     3781 tacaacttca gtgtttatga tgcgacaata tagcagtcaa tttcctacag cactgatgga
     3841 ggctgccaga atcgatggtg ccaatgagtg ggtgattttt acgaaaatcg ctgtaccgat
     3901 tttatcacct acgattatta ctagtggttt acttatcttt atgggaagtt ggaatcaata
     3961 tctatttcca ttagtcatgc tttcagataa agataaatat acggttccct taattatcaa
     4021 aaatatgacg gtttctagta caggtgagcc aattaattat ggcgcaatta tgctaatttt
     4081 agcaacttca gtgatcccaa tggtcttgat ttatgtgtgg gcgcagtcta aatttaagga
     4141 gaatgacatt ggagctgcaa ataagtaaaa aaaataacga atgttaacca tctagttcgt
     4201 tctaggagca actccacatt cataaaaatt aaaagaatgg cgggatatcc atgaatgaac
     4261 agacgaagga atggtcaaga aaaattttga atgtagcttc tgaaaagata gatgcggaat
     4321 tagacagaat ggggaataaa attccatata tttcttacaa cggaaaatat acagacgtaa
     4381 taaaggacag aggaatcgac tggtggacca atggtttttg gagtggtatt ttatggcagc
     4441 tctttcacaa gacaaaacga gaaaagttca aagaggcagc aatacaacaa gaacaattat
     4501 tagaatctgc cctgtaccat ttttcagacg ttcatcatga tgtaggtttt ttattcttac
     4561 catcggctat tgcacattat caacagtctc aaaacaaaca tagtcgacaa ctagggttac
     4621 atgcagctac ggtacttgct ggacgctata atatttcagg gaactattta gtagcatgga
     4681 atgaccataa acctggttgg atgattattg atagtatgat gaatttacca ttactgtatt
     4741 gggcttcgag ggaaactggt gatccgaggt tctctaaaat tgctcggcag catgcagata
     4801 caacagctaa gtatctagtg aggaaagatg gatcaaccgg gcatatcgca gaatttgatg
     4861 cagaatcagg gaaatatatt cggttacttg gcgggcaagg atacaatgag aattcttgtt
     4921 ggagcagagg aaatgcatgg gctttatatg gattcgcgtt aagttatcgt tacacaaaaa
     4981 atccgtacta tctagctata gcagaaaaga tagcagtaaa ttttattcag aaaatcgaaa
     5041 aaactggtta tattcctctc attgattttg ctgtaaaaga ttcgaatatt gataccagtg
     5101 ctggagtatg cgctgcatgc ggtcttctgg aattagcagg atatgtttcc tctgtacgat
     5161 caaaaatgta tcatgaagct gctatcaata tactccaaaa gacagaaaag cactatgcaa
     5221 attggaatat ggatgaagat gggatcattg gaggcggagc agaagcatac catagagaac
     5281 tgacttataa tgtgccactg atctattctg attatttttt tattgaaggc ttacttcgat
     5341 tagaacagtc ggaattagct atttggtagt aaaaagagga gaaaatacaa tgaaacatcc
     5401 agtaccacta ttaaggacca gacaggatta ctttgactta tttaaaaaga taaatcaacc
     5461 actccagccg ttttatcgag caagtggcgc acaaattgat tatatacatc aaggggtagg
     5521 atatggaaat aaaattgcag gaatggaagg attctctcgg gttttatggg gagcggggcc
     5581 cgcaattgac caattagatg aacgatggtt gaaattaatt ctatcaggaa taaaagctgg
     5641 aacagatcct tcccatcccg attattgggg agaaatacat gaaagagacc agcgtatggt
     5701 agaaatgccc gcaatgttat tagcgttata tcatcatgaa caattattat ggaaaaaatt
     5761 agatacagga gaaaaaagcc agattattca ttggttctcg caaatttttg aatatgaatg
     5821 tgcagatggt aattggcaat tttttaagat aattgtcgga acaattatcc aaaaatttgg
     5881 ttatccagtt aaagaatcag cactgaaaga agcatttgat aatatagaaa attgttatct
     5941 tcaaaatggc tggtatcgag acagttcaag aggtcgccaa gattattata atccttttgc
     6001 atttcattat tatggtttga tttatagcgt actagaacca gaaaagccca ttagccagat
     6061 atataagaac cgagctattg atttttctag agactatatt catttttttg cggaagatgg
     6121 tgcaaatgtg ccttttggtc gaagcatgat ttatcgtttt gcagtttcaa gcttttggtc
     6181 agctatgttg tgggccgatc ttttgccttt taaattaggt gaaatcaagg ggttagtcaa
     6241 tcggaatata agatggtgga tgcaaaaaga aatatttgat gaaaaaggta tcctgaatat
     6301 tggttatacc taccctcaat tgacattgac tgaaccctat aatagtacgt tatctccatt
     6361 atggctgaat aagatttttt tattgttggc gctacctgaa gaccatgagt attggacggc
     6421 taaagaaatg cctatgccta ttcgtgaagg aactaaactt ctttcagaag ccaatttgct
     6481 catacaacat gataatggac atacattttt tttgaatgcg ggacaattag ggccaaattt
     6541 ccatacattg accaatgaaa aatatttaaa atttgcttat tctagtcaat ttggtttttc
     6601 gataccgcga gcgaatcaat tgaaagcaga aatggcaatg gattcaatgc tagggattca
     6661 gcgagcggac acatatatca cgaccagttt taagaatgag agcatcaaaa cctatggaca
     6721 gttcatcgtt cgaaataatg tacgagacat tgtatgtaag aaagaggtac ttgcctctac
     6781 atgggaacct actttacagg caaaaatcag aacttggctt attccttttg aaggctggca
     6841 gattcgcgta cacaaagtag aattagatac agaatatgtt ctatatgaga cagggtttgc
     6901 tattgaatgt tctcctgaga aagaaggaat aatcatagaa gaagacaagg aaaactatag
     6961 ggtgagtgaa gctggctttt caggtattat ctgtttaagt gacgatacta ttaaacgaaa
     7021 atccacagct attatgggtt tgcccaatac aaacttgatg acatgggaaa acacttttat
     7081 tcctggatta gagggtacat ttggaaaagg aacccattgg ctcggaacag gagtggttgc
     7141 tcatcaagat cgggattata gtttagaagt atggaacaat cgtcccaaaa ttgattttga
     7201 tgggacaacc gggctaacta tccaaaataa aaacaataaa aaaagactaa aattatgcta
     7261 aaggctggtg gggaaatgct tgataaagtg atgtttgaag agataataca tccagaaaaa
     7321 tggataaatg aatgttataa agaattaatg gaaaaagaat ggaataatga gatcgagcgt
     7381 tgtcgcagac acacggagag gttaatcaaa cgtatcagta acagtcagta tcaattccca
     7441 agcgagtgtc ctataaaagg acgttatcca ttaagagaca atattggttg gacagaagga
     7501 ttttggacag gaattaattg gttgttattt gagaagacgg gtgatccttt atttcaacaa
     7561 acagctgaaa aagcagatat agattttatc cacaggattg actgtcaaat tatcactaat
     7621 catcacgatc ttggtttttt gtattcttta tcaaccgtag cagattataa aataacggga
     7681 aatcgaaaaa tgaaggaagc ttcaattgat gctgcagaaa cattacttca gcgtttttgg
     7741 gagaaggcag gagttataca agcttgggga gatcctagtg atccttcaca acaaggtaga
     7801 atgatcatgg attgttgcat gaatcttcca ttattgtatt gggcgagtga agaaactgga
     7861 aatcaaaaat ataaaaaagc tgctcaggct catattcaaa atgcagcaga ttatttagta
     7921 agagaagatg gttcaactta tcatacattt tatatggatg tcaaagatgg aaaaccaaga
     7981 tatggaagta cgcatcaagg ttatagtgat aattcctgtt gggctagagg ccaagcatgg
     8041 gggatttacg gttttgcatt aagttatcgt tatttgaaag aacaaaaata ttttgattta
     8101 agcaaaatat tagccaatta ttttatgaat cggttgccag aagataccat ttgttattgg
     8161 gatttgatct ttgatgattc tagcagagaa gaaaaagata catctagtat ggtgatcatg
     8221 gtttgtggat tattagaatt aatagaatca ggtctattag acaaaacaga aaagaactta
     8281 taccagtctg ctgtttggaa aattatgcga caattgaaag aaagatatgc tacaacggaa
     8341 gaagaagatg gctttttaac acagtctgtt tataataagc ccaaaaataa tggggtaaat
     8401 acaccttgta tttggggtga ttactattat ttagaagctt tagtgagaat gactagtaat
     8461 tggtcttctt attggtgaaa aaggtaaata tggaattttt gtttgataaa ataaaaaaag
     8521 aaaaaccaaa acagcatgaa tggtttctgt tttggttttt tcgttaattt tcatattctt
     8581 ctaattccaa gcttagttct tcccattccg tcaattttgt ttcttgatct tgtctagcag
     8641 cttctaaatc ttggttcagc tcattgagtt ttacgtgatc ttccaggttt tctggcgcga
     8701 ccatttcttg ttccaactga tcgatccatt catcgatccg cgccatttct tcttcaacct
     8761 gagtaatttt tcgttgaagg ctacggataa gcttttgctg ttctttgctt tggtaataat
     8821 cagatttgtt cgtgggagca ggagcagatt ctggctcatc ttgtgccaac aattcggcga
     8881 tttcttcttc ttcctgcttt ttctctaaat aataatcata gtctcctaga tagactttgc
     8941 tgccattttc agagagttcg acgacttttg tcgcgatccg attgatgaaa tatcggtcat
     9001 gggaaacaaa aagcagtgtg ccgtcataat cgatcaatgc attttctaat acttctttgt
     9061 tgtcaatatc taaatggttt gttggttcat ccaaaatcaa aaaattttct ttgttcatcg
     9121 ctaacttggc taaagcaacc cgtgcttttt caccgccact tagcagaggg attggttttt
     9181 ctacatcttc gccagaaaat aagaaagcac ctaaagcact tcggatctct ttttctggag
     9241 tggtaggatg ctcatcccac aattctgcta ggatcgtctt gtttgtatgc agatcagctt
     9301 gaccttgatc gtaatagccg atggtgacat ttgtccctag cgtttctttt ccacggataa
     9361 aagggatctg gtgaatgatt gatttcagca acgtcgattt cccgatacca ttcggaccta
     9421 ctagagcaat cgcttcttga cgacggatat ctagattgat tggttcagat aaggtgacat
     9481 tatcatatcc gatagcggca tcttcgattt gcaagactac atttccagaa ggcttttcta
     9541 tactgaatag aaagtttgct tgtttttcat ccccttgagg acgatccagc cggtccattt
     9601 tttctaatgt ctttctgcgg ctttgcgctc gttttgtggt agaggcgcga acaaggttcc
     9661 gagcaacgaa gtcttctaat ttatggattt ctgtttgctg cttttcataa gccttccatt
     9721 cacttgctaa ctgttctgct ttcaaatcta gatatttcga atagttacct ttgtaatggc
     9781 gcatttttct tctgcttaat tcatagactt cattgactac tttatctaaa aaataacggt
     9841 catgggaaac aatcaataat gcaccagtat agctttgcag ataattctca agccaggaaa
     9901 gtgtctcgat atctaaatgg ttcgttggtt catctagaat caaaatgtca gggtgttgca
     9961 aaagcatgcg agctaaagct aaacgtgtgc gctgtccgcc tgataaagta tcgattttct
    10021 gatcataaaa tgaagaatcg aatttaaacc catgcaatac ggaacgaatt tcattttcat
    10081 aaccatagcc gtttcgatct gaaaaatcgt gttgcagccg gtcgtattct tttaaataag
    10141 aactataagc aggtgtgtct ggcgtacctt cgctaatgaa aatctctagc tcgcgcatcc
    10201 gctcttccat ttgtctaact tgttcaaaag ctttcagcat ctcgttccaa actgtttcat
    10261 tggaatcaag tcctgtgtcc tgagcgaggt atccgagagt cgctgtttta tttttagcga
    10321 tctttccttc atcaggtgct tcaattccag caatgatctt taataaagtc gattttccag
    10381 cgccatttct gccaactaaa gcgacacgag cgcctgtact gatttctaaa tgtatgtttt
    10441 caaataagac ttctgcacca aaatggcgag caatctgatt ggcttgtaat aatatcatgg
    10501 aatcctcttt tctactttgt gaattctcct caaataaaaa actatttttc aattagtagt
    10561 ctaccataaa ttccattata ctgcgagatt tgcagaaatg ataaataaat gaataaagaa
    10621 taaaatttca caatatctga gcaatggaat tgattttttc ctataaatac tgctatgatg
    10681 gagaaatcaa agatgtaatt agacaatacg tggaggtcat ttcgtgaaag atcatattat
    10741 tccaaaggcg acagctaaaa gactgccttt gtattatcgc tatttaagga ttttaaataa
    10801 tgctggaaag aaaaaagtgt cttcaactga attaagtgaa gccgttcaag tagatagtgc
    10861 aacgatccgt cgagattttt cttattttgg cgaattagga aaacgtgggt atggttatga
    10921 tgtagaagat cttatgcact tttttgcaaa gacattaaac gacgatcaat tgacaaatgt
    10981 tgcgctgatc ggtgtaggga acttgggaag tgctttacta aaatacaaat tccatcaaag
    11041 taatagcata cgtatcagtt gtgcttttga tgtgaatgag gaaatcgttg ggcgcatcgt
    11101 agacggtatc cccgtttatc caatggaaga tatgtcagaa caaatcaaga tccaacaaat
    11161 cgaagtagca attttaacga tacccgctga aagagcacaa gaagtagtca atcagttaac
    11221 ggaagcagga atcaaaggaa tcttgaactt taccgctgct cgattgaatg ctccgtcaga
    11281 tgtattgatc caaaatgttg atttgacgaa tgaattgcaa accttgatct atttcttgca
    11341 ttctgacgca aatgcgcatt ccgaaaatac agcagaataa aaataaaaag cgattgaaca
    11401 aatgaaggca aaactttcac ttgttcaatc gcttttttct gtttggacaa cgattatttt
    11461 gttcggaaga tttcttcata agaaagggaa tcatcttctg ctgtcagttc atctagtaga
    11521 taacggattt ttttaaagtc gatgaaccgt gcttgccgaa agacttcttt tccttgatga
    11581 aaaatcagga caactggcgc agtcagcact tcaaatttac tagctacttc tggtgtttca
    11641 aatgcatcca aatgaagtgc cggaatatca taatgtgtga gaagttcctc tagtcgtgga
    11701 cgaacagcgt gacaaacact gcaattaggc atcgaaagat aaatgacggc aacggatgac
    11761 tgttgcagta tgtcttgaac ttcagtcaag ttagtggttt gtttcatttt tattctccct
    11821 tttatgatag aagtgatatg tatgagacct taatttaatg ggaataacag aaaaagtcaa
    11881 ttgaagtgct ttggtaaaaa ataagagttg tgtaaaaatt tacagagcaa cttgtttttt
    11941 ttgaataaaa gcgtatattt gcaaagcgaa tgaactagaa ataaaaagga ggaaactcat
    12001 gttttctttt attccaaatt cggttttgca gatgagtacg attttcttat cgatcgttat
    12061 tgaagcatta cctttcgtat tattaggctg tcttatctca ggcgccttac aagtattttt
    12121 gaccccagaa cgtgtgaaga gatggcttcc tgaaaatcgt atcttctcta tttttactgg
    12181 ttcgatctta ggatttttct ttccttcatg tgaatgcggc atcgtaccga tcgttcatca
    12241 atttgttaaa aaaggcgtac cagttcacac agcgtttgct tttatgctga ctgctccgat
    12301 catcaatcca atcgtgattt tttccacttt tatcgcattt ggcaattctt ggcagatggc
    12361 aagctggcgg gtgataggca gcttttttgt tgcccttgtg attggtgtct ggcttgccta
    12421 ttttcaaaaa gacgctgtat tgaaagaaaa aatcacgtct aaagtagcag aaaatcacca
    12481 tcatcatgtc catgaagaag aacaaagtga gaagaatgaa ccaaaaaatc agctgatcca
    12541 tcaaactggg catgtgttga cacattcaat cgacgaattt tttgatacag gtcgttattt
    12601 gatcattggc ggattaatcg cagctgcgat gcagacttat ttaccaacta gaattatgct
    12661 aacgctcggc tcaacaaaga ttttagccat tatcatcatg ctgttattgg cctttacgat
    12721 gtctctttgc tcagaagcag atgcgtttat aggatcttct ttgttaagtc tgttcggcac
    12781 agcacctgta gctgcctttt tggtctttgg cccaatggtc gatatcaaaa atctactgat
    12841 gatgaaacga tattttacag gacgctttat tttatcgctg atcggactga ttgcccttag
    12901 cgttattgga ttcacattat ttatttagga gggaaaatat gctgcggttt ttgattttat
    12961 ctgggtattt cgaattgatg atgtacttgc aagtttctgg caaactcgat caatatatca
    13021 atgtccatta tcgctactta gctattcttt caatgatttt atctgctctt ttggctttag
    13081 ttcagctgta tttgtgggtc aaaaacggaa aagcagaaga aaaggcacac gagcacgatc
    13141 atgaccatgg gttgtcaaaa ccttatcaac gtggaatcgc ttatgtactg ctagctttgc
    13201 cagtaatcgt ggggacgttg tttccaacag ttagtctcga cactacgatc gtagaagcca
    13261 aaggatttaa tttcccagta agtaaagaat cagtagggga tccggagatg cagacacaat
    13321 acttaaagcc ggataccagt atttatttca acaaatcaga ctacttagac caaatgaatg
    13381 agttgaaaga aaagtatggg gataagcaaa ctatcaagat cacagatgaa aactatttag
    13441 aagttatgga gctaatttat aattacccta gcgaattcat tggcaaaaaa atcagctatg
    13501 aaggatttgt ttaccaaaca ccgaacgata gcaatacaga tatcttcttg ttccgattcg
    13561 gcattatcca ttgtgtggca gattcaggag tctttggttt gttgacgcat atgccggaag
    13621 gagtaacggt taaaaatgat gaatggtata aaatagaagg aaccatccaa tcagactatt
    13681 atcaaccatt caagcgtgaa atcccatctg tagttgttac aaacgctgag aaagtctctg
    13741 ctccgaaaaa tcagtatgtg tatcgttctt tttaaaagaa caaaataaat aaacatagct
    13801 cagacagctg acacgcaagg acgtcggttg tctggctttt tttgcatgat gtattcattt
    13861 gtttgatatt tattttagaa gcagcatcct gaaagtattt gatatcggtt tcaaaggtgt
    13921 tggaaaagat tgtgaagaga attacaattt attcctaaca tgtatgttga aaagttgtat
    13981 gaaatgattt tgatagtaaa atatcgagtg gaggtacttt ttatggtaac gcgtatattt
    14041 attttggatg atagtttggt ttttgaaaaa atgctagtag aaattttgga agaatccaga
    14101 attttattaa gtccttggaa atttgagatc gttcagggat tgaacgttat acaatttgtt
    14161 gaattcatta aaaataatga aatctattcc tcagatattt ttttcctaga catcgatttg
    14221 aacgcctatt atagcggtat cgaactaggc gaaatgctta gagaaaagaa tgaaaagtgt
    14281 aaaattgttt atctaacgtc tcatccgaat aaagcaataa ctgttattaa tcgaaagatt
    14341 acgcctagtg gttatttgat aaaagaattc acttatgaaa aaaataaaaa aagcatccaa
    14401 agttacttat tgaaagaaaa agaaaactat cctgttcaac atgctacgaa aaaaaggtgg
    14461 acaactgtcc gttatggtaa tagcgatcac tatatccatt acgatgaaat cctttatcta
    14521 atgagcatgc caggttataa aaataaaata ggcctagtat tgaaagatga agagattctc
    14581 gtaaacggca caattaaaaa atataaaaaa tcactcgaat atgagtattt atgtaaagat
    14641 ttaaaagctt atatcattaa tactgaaaac attacatcta ttagcagagt aaacgaaata
    14701 gtaacatttg caaatggaga acaactagag atgggaaaga gaagtattga taaactaaag
    14761 aattttatgc aagaaaagat agaaaatgaa tcattttaaa gaaaacaaag actcatatct
    14821 tacctgttag aagagtgttt tcctgtagaa gagaacaacc caagtagcag cctagtcgtt
    14881 tctatccttt tttgttatgc actctttttg acatttactc caccaataac gaagggtaca
    14941 ataaaaagta ataacaaaca aaaggagtac agaaatgatt gaattcatca atgtaagtaa
    15001 aagttacggg aaaaaacaag cattaaaaga cttgtctctc tcgataagac aaggggaaat
    15061 ttttggattt ttaggacata atggtgccgg aaaatcaact acgattaaaa gtttagttag
    15121 tattattgaa ccatcatcgg gaacgatttt agtagatgga atgaaattga cggaaaatcg
    15181 attgagtatc aaacaaaaaa tcggttatgt gcctgattcc ccagatattt ttttacaatt
    15241 gacggctgga gaatattggg acttgatttc agctgcctat gaattagaca cacaaaaaaa
    15301 agaaaagcgt ttagcagaat tgacagcgct ttttgatatg tatagtcatc aaaatgaaac
    15361 aatcgcaagt ttttcacatg gaatgcgtca aaaaacgatt ttgatcggca cgttgctgcc
    15421 agatccagat atttgggtat tggatgaacc tttgcaagga cttgatcctc aagcagcatt
    15481 tgatttgaaa gaaatgatga aggctcatgc cgcaaaagga aaaacagtca ttttttctac
    15541 acatgtattg gataccgcac aacagttatg cgataaactg gctattttga agaaaggtga
    15601 attgatttat cagggggcag ttagtgaatt attgaatggc tcacctgatg aaacgctaga
    15661 aaagatttat ttgaaaatgg ctggaaggca agcgacagga gatggaccgt atgtataagc
    15721 gacaattatt agaattaatt cgagttaatc ttttatatgt taatccacag ctcacgaata
    15781 aagcaagaga gaaaggaaaa aagggaaagc gattaactat ctatcttttg tctcaaaatc
    15841 ttttttcagg gctgattttt ttactgattt acggatttgc tatgtttgct atgaactttg
    15901 ttaaattgcc aggtttcttt acttattacg tggcactttt tggtatgtta ggtttaactc
    15961 aaagtatatc agtcatttat aatatctttt ttgaaggaaa tgacctgcaa gtatttttgc
    16021 ctctgccttt tggacaagaa caggtttttt cagcaaagat tttggtagtt gctttgacca
    16081 tcattccttt tgtttttccg cttctagtca tgtttatttt aaccggatgg cgagctgatc
    16141 tagggatttt gcttactatc attatctcaa tccttttgtt ttctctattt ttgattattc
    16201 ttttttgtct ggctagtttg attgtttttg gtttggctcg aacagcagtg tttaaaaaac
    16261 atagaaaaac agtttctagt atcttgcttg gcagctcgat gctgatcgca tttgctggaa
    16321 tcatgtggat gaatcatcaa acggggacta ttgaaaatga gttaacagat cggcatccga
    16381 tttcgatatt attgccattc tatgatgctg ccagtcagcc actttctcaa caaggtttat
    16441 tgggttgggg aatactagct atcacggctc tgggattttt ccttgctgta aaacagctga
    16501 tagtaccaaa gcttacagag caactgactg attcaaacag tgagttcaaa ataaaaagaa
    16561 cccataaaaa gaaccaaaat gtgcatcagc ttttatttag ttacaatgct caattagtaa
    16621 aagaacctaa tttgattatg caagttcttt cttcttcctt gttaactcca atcattttta
    16681 ttcttgcttt tgccctagga ggcgaaataa aattaactga tttagataat aagttaatcg
    16741 gtgttgtttt cctggtaggg atcgcattgg ctttcttaac agttaatcag acttctttta
    16801 tcagcaatct tatttctttg gatcaagaaa attttctgtt tatccgttct ttgccaatct
    16861 ctatgaacca atatttaaaa gaaaagttca gattcggatg gatattgcag agttttttaa
    16921 ctggtgggat cgcattatta agcggactgt cttttcagct gcctttgttt tttatcccta
    16981 gcttgctatt tggttcttta ttcggctgct acttattgtc actacgttat ttcgctagag
    17041 attatcggtt gctgctgctg aactggacca atattaacca attatttaac agaggttcag
    17101 gaagtttggg tttagtcgca actatgatgg gttcattgat catcagtatt attcttatcg
    17161 tcttatacgg aatgttggct ttgttttatc cattttggtt agtgaatatt cctgtgatcg
    17221 gtttagttct aatactttcc gctctatgga tcgtttatta tcaacgaacg ttttggaaaa
    17281 aatttgaata aagaagaggg gccgtgacaa aaatcatgtc gcagcccctt ttttacagat
    17341 agagggtgtt caaaagtcag tttctcagta ttaagtcaat tttatgtaaa aatgtgagaa
    17401 gctattttta caaaactttt tttaatactc ataattactt cttattgctc ctgcggttac
    17461 ttgtcgcaat gtttgtgaga tattttgtca tcgctcaagg ctgaacgtct ctttcacaac
    17521 cttttctttg ttacacttcg tttatttaaa aagccgtttt tttcgtttca tcatgattgc
    17581 gtcacgcttt actttgaaat tacggatgtt atggttaaag acttctgata atacaagacc
    17641 taaagcgatt gctccagcaa tcataattgc ttggaccgca taatgagccg cttctcgata
    17701 ggattcagta acgagatttc gaaccgcttg ataagctaaa ccaccaggga ctagaggaac
    17761 catcccggga atattgaaaa tcgttacggg cattttcaaa cgtttagaga aaagatcgct
    17821 gatgaatgct actcctaaag aaccaagcaa tgaaccaaaa gcagcagatc cgcctatttg
    17881 aacacagatc cagtagatca tccagccaaa agcaccagaa agaccacaac aaatcaatga
    17941 acgcctaggt acatttgtaa taacggcaaa tgccgcagtc gctaaaaaac taaaagaaaa
    18001 ctgaataaat atcgtcccca tacttgtgtt cctttcctaa taaaaaagct ggaaaataaa
    18061 agcaatcgca aaaccgatca tcgtagcagt catcatcgct tctgttccgc gagaaactcc
    18121 tgagacataa tgtccagcta gcaaatcacg gatagcgttc gtaatttgaa ctccaggaac
    18181 taaaggcatc acacatccga taatgattag atcctgattg cttcctaagc caaaacgtac
    18241 gctgtagata gcggctatgc caatcaataa agaagataaa aattccgata aaaatttgat
    18301 tctaaaaaat ttcaaactat atagataagc tgcgtatccc aatcctccaa tgactaaagt
    18361 cacaggtaaa tcggtcatct ttccgccaaa taacaccata attgttccac tgatcactgc
    18421 cgcgctaacg atttgcaacc agacaggaaa gaaagaacgg tctcggtcga ttgcttgcag
    18481 ttcgcagtaa agttcttcta atgttagctc tgaagcaaca tatttacgtg aaagatcatt
    18541 tacttttact actttttcta aattaatcga acggttggta atttgttcca ttcggatggt
    18601 cgaggtacga tcaagaccaa taaagagacc ggtttgagtg acatagctga ccaaacggaa
    18661 atttccagaa gctaaagcaa ttcgactcat cgtatcttct acccgataca tctccgcatt
    18721 gctttccatc atgattttcc cagcgagtaa acaagtgtcc agcaacagat ctaattcttt
    18781 tcccgtcata gcatcttctc caataattga ttgattagtt ttattttact acaaaacata
    18841 ccagaagcgt aaagaataaa aaaacttgag gaaggaataa aagaggcgta tactgttttt
    18901 gtattgcggg tgtagtttag tggtaaaatc ccagcttccc aagctgatgt cgcgggttcg
    18961 attcccgtca cccgcttaaa ataggttgtg aaaaagctgc tctgcaacaa gtaataagaa
    19021 cagccaagca aaaacaactt cttatgtttt taacttggat ggacttatta ccgcagatgc
    19081 aagcttttgc gcaccgttta ccaagcggtt gtgaaatcag tgtattgtct tgagtatgaa
    19141 gaaaattttt gaataataat aaatacctta cagatagagt ggtgactgat tagagacgag
    19201 gaatccgtct cttttttgta tttttaagcc atcttaaaac aggttttatt ttttgctgtt
    19261 tctatttctt tctagtattt atttataggg gaacatatgt taagatttag ggagctgtta
    19321 gtaaaggagt aaagaaatga gcaaaaaaac aaccaaggcg atcgtgggga tcgtcgctgc
    19381 tgtgattgca ggggcattcg gtgtttccac cacacaaaaa gattcagata tctttcagca
    19441 gattacaact atttttgatt ccacaccaca agcttctcaa gtagcggatg tgccagaata
    19501 tgatggaaaa catcaagaaa tagaaatcaa tgggaatcag ccggaattta caaaagagga
    19561 tcttagcctt tccaaaggaa catgggaaag ctattcaaat attgataaat tgaacagagt
    19621 aggacaagcc aatgctatgc ttggaaaaga tttgttccct actgaaaaga gagagccttt
    19681 gtatattgac ccgacaggtt ggaaacaaaa gaaattaagt gacgggcaat ggttatataa
    19741 tcgcagccat ttaattggtt tccagctgac tggtcaaaat aacaacatta aaaacttgat
    19801 gacagggaca cgttcgttga atgctccata catgcttgct cacgaaaatg atattgcggc
    19861 atatatcaaa gaaacaaatc atcatgtacg ctaccgtgtc actcctcatt ttgaggggaa
    19921 tgaactggta gccaggggag tgcaattgga agcagaaagc attgaagaca aaaaaattga
    19981 attcaatgtc tttatctaca atgttcaaga tggctacacg atcaattatg agacaggaca
    20041 agcaacaaaa acaaaaaatt agtagcagct attcgaattt tttatgaata ttatgaaaaa
    20101 attgtgtaaa aacatacatt caaagaaata tagcgtatac tttatgaaaa gagtactttt
    20161 gataaagcta cagaaaaaag gaaaaaaaca tacaaatagt gtttcttaat ttattcggaa
    20221 gtttggaata tttgtttgta gttttccctt tcttcgagta aaataaggtt gtagaaggta
    20281 agctggaatt accttgcggg aaagaggtgt agaatcatgg gttttacaga tgaaaccgta
    20341 cgttttggtt ttgacgatag tcgcaaaaaa gaagtgagcg aaacattagc gattgtttat
    20401 cgggctttgg aagaaaaagg ctacaatcca atcaatcaga ttgtcggcta cttgctttct
    20461 ggagacccgt cctacattcc tcgttatcgt gatgctcgaa atttgattcg ccgtcacgaa
    20521 cgcgatgaga tcatggaagt aattgtcaag gactacctct caaatcatgg agtgaattta
    20581 tgagagtaat gggcttggat gtcggttcta agacagtagg tgttgctatc agtgacccct
    20641 ttggctggac agctcaagga gtagagatca ttcggatcaa tgaagcagaa gaagagtttg
    20701 gctttgatcg gttgaaggaa ttagtagcac aatatgaagt agaccggttt gtagttggat
    20761 tacctaaaaa tatgaacaat tccatcggac caagggcaga agcttccatg tcatacggag
    20821 aaaagatcaa agaactattc tccttgccag ttgattatca agatgaacga ttaaccactg
    20881 ttcaggcaga acgtatgcta gtagagcagg caaatacttc aagagccaaa cgaaaaaaag
    20941 tcatcgataa attagcagct gtgatgatct tacaaaatta tttagacggc tcaggtaagc
    21001 aattatttta ataataaaag aaaagaggta atcttatgac agaacataac cattcccacg
    21061 accatgaagg acacgaacac atcacattgg tggatgacca aggaaatgaa acattatatg
    21121 aaatcctact aacagttgac ggacaagaag aatttggacg taactatgtt cttttatatc
    21181 ctgctggtgt ttcagaagat gaagatgtag aattacaagc ttatgcatac atcgaaaacg
    21241 aagatggaac agaaggcgag ttagaacaaa tcgaaacaga cgcagaatgg gacatgattg
    21301 aagaagtatt caatacattc atggcggaag aagaagaata gactataaag ctaggagaaa
    21361 ccttgatatg atagggtttc tccttttttt attttttact gtatgccata ttgtatgcca
    21421 tctagtcaat atttaagtgt tgtactaatt tttcaacagt cttttctttc gtttcttttg
    21481 ccaagtgacc gtaacaatcg atcgtctgac taatttttgt atgccgtaat ctagtttgaa
    21541 cttctaccat agtagctcca gcgtccaaga gcattgaagc atgagtgtgt cttgctacat
    21601 gcacaccgag aaatggaacc atagcttttt cacaaatagt aatcaatctt gcataaatag
    21661 cgtggtgtgt gattagttct ttatagatac cacaaaaaat catagtcgga tcagttacac
    21721 caagtgtcag catatatttc cgctgattaa tctgccattt ttttaatata gaaattgttc
    21781 tttcatctag tggaagaacc ccagcactac aatcagtttt aggatcactg attttatagc
    21841 catttttgga ttggcttagt gttttattga tggtgaccgt attttcttga aaatcaatgt
    21901 ctgaccaatt caatgcaagg gcttcactga tcctaacccc gctataaaag agaaagcgta
    21961 gtaacgtttt atcaaagtcg tttcgataag tttcaggttt gctgtttagg taatcaaaca
    22021 aggtgatcag ttgttcttta ctgtaaactt tgaccttttc tttatgctta gtggtggttc
    22081 ttttaggcat taatacgttg tctagtggat tagtctcaat aatcccaaga ttgatagcgt
    22141 agctaaaaat acgcttagaa gttgaatgaa gtaccttgta ttggtcagta gtttcagccc
    22201 agctatttac aatttgttgg gcttttttta ccgtgacttc attcacacga agatttccat
    22261 attttggaag aatccacttt ctaaacttga tttcagtttt catgaaggtg gcttctcgga
    22321 cagtggtttt atatgtttct agccacaagt cataaacttc tttaaacgtt tcgttgttcg
    22381 cttttttaat accgttttta tcgtagtcca cttgcaagcg tgacaaggca agctgggctt
    22441 ctttttttgt tttgtaacct cgtctacgaa ccttatcagg tttgccagtc attttgttca
    22501 ttccaagata ggcttcatag agccaaaatt tacctttttt agtttcatat tgcttgaatg
    22561 ttgccataat agttttccct ccatagtgta gcaagccgtg ggggcggttg gaggaggcag
    22621 tttattatta ttttgaatag tgtttattta gctcagtaac aagagctgtg aactctttta
    22681 gaagatcatt ttcataaaat tcttgatact cttttttctc atctgtattt ttttctgaat
    22741 cattaattaa tgagtcatat cgtaaaagcg tatttagaag agctgcgaga taagcaattg
    22801 gggtactccc gtcaggaaat tcaattcgag atcctgtttt aagatatttt attgcgtttg
    22861 ttaaataaat cttctgtgag acagtcaggt tatcttcttc gatgttattg atagcattag
    22921 aaagattaga atcaattata gagttatttt tttcaatagt ttttttcaat tgcttttcaa
    22981 gcacatttcg cttaatttcg ggcatcatat gcatatctct taatgtataa gatccgttta
    23041 atagataggt agtggataca tctaatacct ttgaaagttg tttaagcctg gtgttattgg
    23101 gaagattata gccattttcc caattactaa cggcaccttt actggccggt ggggataatt
    23161 tacctccaag ttgttccatt gagagtccct tttcaattct agccagtcta attctttgtc
    23221 ctgtctccac tgcttcgggg tatttttttc tagccacatt ttcaccacca ttcatatatt
    23281 ttctctacta tatcacgatt taaatatgtg tgaaacataa aagttcaaaa aagtatgtta
    23341 aaattcttgc aaatgaaaag gtatgatggt atattgagtt tataagaggt atagaaatgt
    23401 attcaagagc ctcttgcatt tttttaaaat ctttgtatag aaatgtatgc taacgagggg
    23461 ggataaatat ggaattaaaa attgacaaac tcacgcttga tgatccagct tttaaaatta
    23521 ttgacgatcg agttgttcaa caagtagagc aaactcaagg aacgttactt tcattattag
    23581 aaaaaaagta ccagtttcca aaatatatga acaaggcaca agctgctaag tatatgaacg
    23641 tttcatacaa tactatgatg cagaaatatg ttcctaacgg tttaaagttg attatagttg
    23701 atggagtcgt aagaatttca caagatgaat gtgatcgctt tatggaagaa taccaaaaat
    23761 aaacgcaagc cgtgggggca aagggattaa taggaggaaa taataatgga atcgacacaa
    23821 aataaagcaa tcgaaaaagt attatcaaca attcttacag aagacacagc ggcagaatta
    23881 gcaaacttgg aaggcaaagc gctagaagaa acgtttgaat gtttgtacga acaaatggac
    23941 tatcaaaaat tgctaccaca agaaccaacg gtaagcggag tattgcgtgg cttgaacgat
    24001 ttagtacaag cagagtttaa agaacgactt tctattgaag agtatcaaga aattttgtat
    24061 cagcaagttg agttgttagc gagcttgcta gggcttgaac tggaggacgt tgaatgaaaa
    24121 ctgaaatttg gaacggacac attataagat ttgtcgatat taatgatgaa tggtgggcag
    24181 tggcgaaaga tgttgcggaa gcgttaggtc ttaaacaggt tactagagcc attcactctt
    24241 tgcctaaaga tggggttact actagtaagg tcatcgattc attgggcaga acacaagatg
    24301 taaacatcat caacgaaaaa aaatatttac cgcatggcat tcaaaagccg taaaaaagaa
    24361 gccgaggcat ttcaagactg gattttcgac atcatcaaag aactacgcca gtccacagga
    24421 cttgaaggct ttcaagtatt ccgcatgcta gacaaagaac accaaaaaga agcaatgact
    24481 aaattaagcc acgctattac tgaaccaaaa ccagtagact acattaaagc gaatgtgata
    24541 gccaataaag ctgtatcaac tatttatggt cattccaaaa tggtaaaaaa gaaagatatg
    24601 actcctgaaa tgttggttga tcgtgaacct attttagatg agacagtaga attaatgact
    24661 gttaaagaaa aatatgggtt gcagttttca gtgagtgaga aaatttataa tcgttctgct
    24721 gaattgcaga caacttagga gcattaacat gatcgagaac aaagaaaaag ccttatccat
    24781 aacagtcttg gcggacagga taaaggcaac ttaattggga taaaacccga tattctttgt
    24841 tccgattata ccatattaaa aaatcggagg gaaccattat gaaacgaaac ttaattatag
    24901 gaagcatttt aatcggcgga attttcggct tattaccatt aattgcaaaa atagctatct
    24961 ttgcaggatt agcgttatat ctcttcactg cttatgacga gtacgactat caattaagaa
    25021 ctggagggca taagtagatg tacaaaacaa aactattaaa ccaactcgat agccttgaat
    25081 tagaggaaat taatcaaggg atcgctgaac tagaaaataa tatcgggaaa acctactttg
    25141 gaaattcatt caatgaaaaa ctaacagttt tgtatgtact caaaaaacat gcagaacaca
    25201 aaataatttg tcgagaaatc aacgagctaa aaaatcagat tttaaccgca tggttaaaca
    25261 ttactgatat gcaagaagca agggtgaaaa catttaatac atgggtaaag tatcagaatc
    25321 aattaaaagg tgctgaattt gttcgggatg ggttgaaata cgaacttgag cagttgaagc
    25381 tcatggaggt gtctgaatga tttataccag tattttgaaa aaggaggaga ggtttattgg
    25441 caaataatta tcaagcagca acggaattac aaaagtcggg atggtcaatt tatcccttat
    25501 ctcctggaac taaaattcca ataaaaggct ctagtggttg gaatgatgca actaacgatt
    25561 ctaatcaaat taatgaatgg tggacggaag atgcaactag aaatattggg ttaatgcttg
    25621 aagatcatca aatggtggta gttgacctag atcaacattc tgaaacacga aacggcgttg
    25681 agaattataa aaaacttgct tcaatgtatg atccatttcc ttctacttat acggaaatca
    25741 caccaggtaa aggaatccat ttcttcttta aaattcctga tggtgtaatt atccaacaag
    25801 aaacaggtgc atttgctgat atcttcgggc gtgatgataa aggtaaaagt ttaagcggga
    25861 ttgatattat cactaaagga gttccaatag cgccaactat gattatttca agaagtgttg
    25921 ggagagcata tgaacctatt gaaaacgtat ctctaaataa tattgcacca gctcccgatt
    25981 ggctagtgaa cctactgcta aaaaaaccgc aagaaataaa gactcaatac cgacccgtag
    26041 caaaaacagg gacagcaaaa aaattagata tgattgttca aggcgctggc gaaggtagta
    26101 gaaacgatca tttaactaag ttatgcggtt ggttactatg gcacggtgta gataatcaaa
    26161 ctttagcaga attgatttat acagcaaatg cgtacaatga accaccgcta aaagataaag
    26221 aagtgaatca aattattcga tcgatgatta aaaaagattt gagggggaaa gctcatggct
    26281 cataagcata aattagatag tattttagac tttcccgaag ccagcgagcg tgaagacaat
    26341 atcattgaat tgaaaacctg gatgtcacga ttaagatgca ataaagacga tcagattaaa
    26401 agtaacagtg ttgtgaatgc agaattgatt ttaaccaatg atagcaattt agctggaaca
    26461 attgcttata acgaatttag cggatacatt cacctattaa aagattctcc ttggatcaat
    26521 cgtaccgctg gagaatggga agacagtttt gaggatgcct taacggcata tattgaagaa
    26581 aactacaatg tagtttttga tgacaacaaa atacacaaag cagtagtaaa tgtggctaga
    26641 aagaatgttt tcaacccagt aaaagaacgt atcgaaaaag ttaaatggga tcaacagcct
    26701 agactggaaa cactgtttat tgacctttta ggggtagacg acaaccttta cacacgagaa
    26761 gtaacaaaac gttggatagt cggatcagtc gcacgtatct ataaaccagg aataaagttt
    26821 gagatcgttc cagttttgga tggaccgcaa gggattggta agagtaccgt tccagcatta
    26881 ctttacacgg atgatttttt caccgattca ttagattcgc taggtgaaaa gaaagatgat
    26941 tatatgcagc ttcaaggcaa tgtaattatt gaacttggag aattatcttc aatgaacaaa
    27001 actaaaattg aacagataaa aaacttcatc agtgccaaga tcgataaaat tagacctcct
    27061 tatggtcgga atgtcatagc ttgggcaagg caatgtgtat ttattggaac gtcgaatgat
    27121 gggcagtatt tgaaagatga tacaggaaac cgccgttttt atcctttgcc ttgtaagaat
    27181 aagccaaaga tgaatccgtt taaaacaaag gatgaatatt ttcttcaagt gttagctgaa
    27241 gcaaaggtgc tttttgataa aggacaacga atctatttta gtcccgatga agataaagaa
    27301 gtcttagaaa ttgcaaaaga ctatcaagag gatgccaaga tggagaatcc aattaaagaa
    27361 gcaatcacta aatatttgga aatggaaatc ccgtataaat gggaagaagc tccagcgtgg
    27421 gcgagacgtt cttattatca gaactatcct gacagtaaat gtaatgagaa aaatttacag
    27481 catttctata atggagctac tatgcaacaa gagttttatt tagttgatag cgtactcacc
    27541 gctgatattt tggaagctgt ttttgataaa caagcaaagg atttattgaa tggtcgatca
    27601 gatgcagaaa ctaaaaagat tgccttgatt attagcagta ttcctggttg ggaacggaag
    27661 caactgccaa aaagaaataa acgacgagga ttttacaata agtcaaatgc ctatgaaaat
    27721 aaaaatcgag ctggaaagta caaaaagtaa cgggtagtaa cgggtcagta acacctagcc
    27781 cgttactagt ttacccttac tgccccaagg gatacagcaa tttggtaacg gggtaacacc
    27841 ttatttcctt aaaacttttg aaatagttag ctaatatgct ggggaataat gttactggat
    27901 tttacccgtt acctgttacc aaaacgctag aacccttgtc cccgttggga ttgagtggta
    27961 acgggtacct gttacgtagg tgttaccacg tgttacgaat caaggaggaa agtaaatgct
    28021 caatattatt tcaacaaaca aagcaccgaa ctttcaatat acagatgaaa tggatcggtt
    28081 tctaatgaac acgctggcat ttagtgtggg attggtaaca gaagattatt ccacatttga
    28141 tccagaagta ttaaagataa tggaagaaga acctgattgg ctgcaggagt cagtagcgtg
    28201 gtgtcaatcg ctagtagtgg gatcattggt agatagtggc aactatgatg atacaggtga
    28261 gctaatggat gaatttaatt gtctgcttaa cctatatgat cgagcgagac aacgagagct
    28321 tacatcgaat gaggataatt tgtttttaaa catccatgat aagttcttgg ctttgctact
    28381 aacagatgat gaattgataa ctaatttatt ggaggtagaa taattatgca tatggaaagt
    28441 attagatact tcgatggtga gaaacacatt gagtattcaa acactcaaga agatgtagac
    28501 tttattagtt ttgaggaaga taaaatcact aaagttaatt ttaaagacgg aacgtgcttg
    28561 aagatagttt cgccttatat tgaatacaaa tcaaaatggg aacaagatga ttctgacgaa
    28621 cgagatcgct ttggttggta atctatggca gttaaaaaac aatgtaatca tgctggatgt
    28681 aaaacattga ttgattatag gcagaagtat tgtgggaagc ataaagcaaa gcaaacggca
    28741 attaagcgtg aagaaagaaa gcaaagcgaa ggtaaatatt ttcaattcta tcaaagtaga
    28801 acatggcgaa aggcttcata cttgtatcgg ttaaatcatc cagtatgtga ggattgccta
    28861 gaagaaggct tgataaggaa agctgatgtt gtagatcaca aaattgaact aaaagatgat
    28921 tggaatagac ggttagatga aactaatttt cgttctttat gccatgcaca ccataattcc
    28981 aagacagcca atgaaagaca aagacgagaa aagagtaccc tttgagttaa gggggcatag
    29041 tgtgaaactt actgacaatc gatgcctact cattttggta caaataaccg ttgtgaaaag
    29101 gcataagggt aattacaaat aacgattaca cttgtaatca caagtgaaaa agtatataat
    29161 gaaagtagga gatttattga aaaatacaat tgttattact gataaaacag ggaaacagcg
    29221 caccattgag ttacctcatt ttggtcaagt gaccattcaa atgcagaacg gaaaaatcat
    29281 ttatatagat aaattagata aagaaaaatt ctgatcgaaa aacgaaggaa tgtgagctta
    29341 attgcttacg ttcctttttc ttttgtctga aaggaggaca acatgggaca ccctaaaata
    29401 ttacacgata caaaaggcaa tatatcaagt gaagaaaaag caatccgtgt ggacgctaga
    29461 gaagaattgt tcaagcagca accattaata aatatcacgc cccctgactg gatggcagcg
    29521 agcgctagaa gtgaatggaa tcgtatcgta ccaacattaa aaaaagatta tccattgagt
    29581 gaagcagatt atggttcatt ggtagcctat tgtttagcct ttgctcgaat gaaaacagcc
    29641 gaagccgaga taagaaaatc aggtacgttt atcacatgcg aaaacggagt aaagaaagcc
    29701 aatccagcgg ttcgggttca atcacaagct atgagtgatt tgaaaaaaca agccacctca
    29761 ctaggtatga ccttagaatc acgatcaaaa ctagctttga acaaggttaa aaatgatgaa
    29821 cccgaagacc cgttcaaaga gttgatgaat tcatgaatga ttacattgag aaagtcctat
    29881 caggtaagct gattgcaccc aagaaaatta tccaagcgtg tgagcgtcat ataagcgatt
    29941 tggagcgttc taagtcagat agcttccctt atgtgttcga tgaagaacaa gccaccaaag
    30001 cgattaaatt cattgagttg ctaccatcta cggacggtaa agcaatcaag atgttaggat
    30061 ttcaaaaatt cattctaggc agtctttatg gctggcgtac taaagaaggg aattacaggc
    30121 gattcaatcg agcgtttacc agtatgagcc gtaagaatgg gaaaacgtat atcgcaagtg
    30181 gcatggctgc caatgcgttg attatggaac aagaaccagc agaagcaagg caagtattgt
    30241 ttgtaagtaa cgctttgaaa caagctaaat tgggctatga tatgctgtct aattcactta
    30301 gaaacgtggt caagtctagt aagtttttaa gaccacaact gaaaattatg aactctaaga
    30361 ttcagcactt gccctctaat tcgttcgcta tggcactggc tagtgaaacc agcacgctag
    30421 atgggtttgc accaacaacc gcaattcttg acgagtggca cgaagcaaaa actcgtaaaa
    30481 cgtacaacgt cattaagtca ggaatgaccc aacaaaagaa cggcttattg tgtgttatta
    30541 gtaccgctgg gcttgattta aacgttccta tgtacgagga atacttattg ttagatcgtg
    30601 tgctaaaagg cgaagaacaa gccgacaggt actttatagc gatatgggaa ttggacgatc
    30661 ccgaagaaat tcacgatcaa gagaaatgga tcaaagccaa tccgattttt gaaagtgaag
    30721 aaatcaaaaa agtaatgatt ccaaccattc aagatgatgt gaaccttgct ttgaaacaaa
    30781 ataaccttaa ttctgtattg gtgaaaaact tcaatttatg gagacaagcg agtgaggaca
    30841 gctatatgat tgctgaagac tggcaggcaa ccgaagtaga accacaagat attacaggca
    30901 agcccgttta tatcggagtg gatttatcta aaacagatga tttaactagc gtttcatgga
    30961 tcgtaccgct agataacggc gaactttatt gtgattcaca tagctttgta gccaccaaat
    31021 atgggcttca agacaaagaa aagcgcgacg gtttgcctta tcgagaacta gaaaaagctg
    31081 gtgagtgttc cattactcaa ttagaaagtg gaatcgtgga ctatgaccaa gtatttcagt
    31141 ttattcaaga tttgattcaa gaaaatgatt tggaatgtat ggggatttgt tatgacccgt
    31201 ataacgctaa ttcgcttatc agtaaagctg aaaaagccaa ctacccaatg ttagaagtaa
    31261 gacaaggaac gattactcta aacgttccga cccgaacttt tagagaacaa gtttatgaag
    31321 gcaacgttat tcacaataaa aatacgattc tcacccatgc agtgaacaac gctattttaa
    31381 aaacggataa caacggcatt cagattaata aatcaaagaa cagtaacaaa attgatccaa
    31441 tagctgcatt aatcaatgcc tatgtgtttg caatggatta cttcaccaca acggaaggag
    31501 cgaaagcaga caatgaattt tatacaagtg aagaattttc tttctaatta cattcatacc
    31561 gttcttttac ttcttggatt ggtgtgtgtg ttggttgcta tcaccttact aacaaatgtc
    31621 tactatggct tgttagcgct gggcatagtg cttattgggg tagcggtcat gctaaataca
    31681 gaacagaagg gaggttaaaa gatggcattt tttaaagcga gacaaaatac aacgggagat
    31741 cctttcctac aacatgtggt atcaatccaa tcggatgatt acaccaccag ctttacaagc
    31801 gttcgtgcat taagaaatag tgatgtgttt gcagccgttc gaattattgc tagtgatatt
    31861 gcttcaagtc cgattcaatt ggtcaaaaac aatatgccac aagctgataa tgaactggtg
    31921 aagttactaa acgagaaacc taattcagaa atggatggtt ggcatttaaa gtttgctttg
    31981 gcggtcaata tgttgctaaa cggtaatagc tttgcagaga ttaaacgcaa cggtgaaaag
    32041 gtagaagaaa ttcacttatt acctaactca agtgtaacgg ttactcaatt agataatggt
    32101 acgttgtctt atcagattgg tgataaaaaa agacgtgtga agtctagcga tattttgcac
    32161 ttcaaatatt tcacccaaga tggtttaaca ggattaccac cactttatgc gttacgtgat
    32221 gaactgaaaa ttcaacaagc tggcaaccgc acattgcaca atttctttac tcgtggtgtc
    32281 agtgggtcag gtattttgaa ggttcataag tcagatttag acggatcagc aaagaacgca
    32341 attcgtgaaa agtttgaaga agccaacggt tcaagtagtg gagataatgc tcttagaacg
    32401 atcattcttg atgaaacaat ggactacaaa acattagaaa taaacaccga tgttttgaag
    32461 cttatcaatt ccaatgattg gaacacaaaa caaattgcca aggcgtttgg tgtaccgatt
    32521 gagcgtttag gcgttgaaaa tgaacattct agcaccgttc aaagtaatct ccagtatatc
    32581 caaagtacgc tgattcatta ctttaatgtg tttgtgagtg aattagatac gaaacttgaa
    32641 accaatatcc gctttaattc cgatcagtta ctagagactg atcctgaaac aaaagtaaaa
    32701 aatgtattgg atcaggtcaa agggtcactt ctcacgatta atgaggggcg gtcgaaaatg
    32761 gggctacccc ccatggatgg tggcgatcgt ttactagcaa gtttgaactt tacgtattta
    32821 gatacgttgg agaaatatca attaaaagaa caggaaggag ttacaccagt tgaataatga
    32881 agaagaaaag gaaaaacggc tgacagaaga agctgagcta acagccgatt ctcccaaagt
    32941 agagaaagaa aatgaaaaac aaccaacaga cggcaaaact atttcaggct atgcgttgaa
    33001 attcgggcaa ccgtcaaaag atttaggcgg ctttgtagaa gtgattacac ccgaagcatt
    33061 aaaagaggtg gatttatcaa atgtgttctt attatatgga cacgattatt caaagccact
    33121 agctagtgtc aaagcaggta cgttgaaatt gaatgtcgat gatacaggat tacattttga
    33181 agcagagcta accgacacga cctattcaaa tgatgtgtat gagaatatct caaaaggggt
    33241 tgttgattct atgtcctttg ggttcgtgct tggattagat tcattcgaca aaaaagaaga
    33301 tggcacaatt gaacgatcaa tagataaaat caaagcactt aatgaaatta gtgtcgtgac
    33361 cgttcccgct tatgattcat caaatgtcca agtaaataag cgttcttacg aatcgtttat
    33421 gagtaacaac caagcaaaac aaacaaacaa tagcttagaa tccacttcta aagcacaaaa
    33481 ggagagtaat aacatggaaa aaactttaat cgataacgaa aaaacagaat tgcgtgggta
    33541 tgaagaatat atccgttcac aaggcgaagt gcgtgatgga gtcactactg taaatgcagc
    33601 ggcagttgtt cccgaagaag taatcggtga agtctttgat ttgaaacgtt caaattataa
    33661 cttagctcaa tatgcaacag taaaaacagt atcaaatgga caaggtaaat atccagtagc
    33721 aactaaccaa caagcagtgt tagcaacaaa agctgaactt gctgaaattg gtgatattga
    33781 cgctgaaatg tttacttcag ttgattataa agtagaaact cgtgctggta agattgcctt
    33841 atcaaatgag gttgtggaag attcagcagt gaatattgta caagaggtca aagatcagtt
    33901 agcgaaattg gtagaaaaca ctgacaataa gcatatcatg gatttattaa aaacattcac
    33961 taagaaaacg gctgctacgt tggacgattt gaaacaacta tacaatgtgg cattagaccc
    34021 agcattaaat aaaatggtaa ttctaaacca aagcggatat taccacctag atacattgaa
    34081 agattcagat ggacgttaca ttttacaacc cgatgtgaca gcacctagtg gtaaatcatt
    34141 attcggtatg ccagtagtat tgattgcaga tacattgttt gccaatccta aagctggaac
    34201 attccctatg attatggggg atattgcaca atctatcttt gttgctcgta gaaatcaagt
    34261 aacgactcaa tgggaaaaat tcgattacta ctcacaagga cttgcagtga tcgttcgcaa
    34321 cgattataag aaaattgatc aaaatgcttc agtgtatatt gagtttacgc cagttgtaac
    34381 accaaaagta tagaaaaata ttgggcggcg gtttatccca ctgccttttt ttcattagga
    34441 gagataaaat ggtaattttg gatagcataa aaaaaagtat gcggatcgat cacactattg
    34501 atgatgactt tattcaacaa ttgattgata cagcaggcga atatataaaa agtgctattg
    34561 atagtagcgc aacggataaa gatatggata attatcagca atttgatttg gcggtgtcat
    34621 tacttactca acattggtat ttgaaccgtc aagaagccag cagcgaacgg ataccagtaa
    34681 cggtacaagc attagtacaa caaatgagag gtgcttatta tgccaatcat taagaatgta
    34741 aatgaattga ctgagagagt caagtttaaa aagacaaaac gggtaaaaga tgaagatggg
    34801 cagatggtgg atagtgaaga aactgtattt gaatgttggg ctaatgtgcg ttcacaaatg
    34861 ctaaaagacg tgcttgctag tgtaggtacc attcttgaag gaacgttgac gtttattatt
    34921 cgatacgatc aagattatga actaacaaac gatatgaaag tagcttggaa aaacaagagt
    34981 tataaaatta tttcaatcaa tgaaggaacg gcgtttaaag attatacaac gatcatagct
    35041 aaacagattt cttagaatga ttacacttgt aaacgttgtg ggtatttagt ataattaagg
    35101 tagtaaatga agggttagct gcctgaattt tgcaagactt agctagtcga aatctattgt
    35161 tggactgaaa attgtaggtg tggttgcaaa catgctggac taagaaagat gatagatgat
    35221 gaacgttcaa ttttaataca ggcatgctag gactgattgg atggttttgg caagctgatt
    35281 gtgtagaaat caaagaacac gtctgttgtg gacgtgtttt ttttgttata attcaaaaga
    35341 ggtgatttaa tggacaaagc agagtttaca aatttttatt ggaagtatta tctacattta
    35401 gaaaatgagt ttattaatac cacagatttt gtgatgctcg atgagaataa tttcaaaaca
    35461 ttctctattg aatatcaaaa actactacta gcgattggtt cggagtgtga gattattttc
    35521 aaagaacttt gcggatttga tagcagtagc aataaaagca taactgattt taaagctgtt
    35581 attaaatcta gcgaattaat gtgtttggat aatggtgtta gggttatgaa ttctttaaca
    35641 ttgacttcac taaaaccttt tggagatggg tggcctgaag atacaccaga ttggtggaaa
    35701 atatataaca gagtaaaaca tggtcgttct tcaaattata aggaagcaaa tttggaaaat
    35761 gtgttgtatg ctttagcaag tctatattta ttagaggaat atttgcataa gaaagtaata
    35821 ctagaaggtg aagtagactt tataagtcca gagtcaaatc tatttacgtt atcttgggaa
    35881 agaaaacatt cgtcgatgca aaaagtgacg tttgaaaaag tggattcaaa ttatgtagct
    35941 ccactcattg attttaaaga acaagaagaa atttaaaaac ccccacggct tattgaagtt
    36001 tttgtatgcc atattgtatg ccatttggtt taaatcgctt gaaaaatagt gatagataac
    36061 tctaattcat ttagtcataa acgctgtttt gataaccttt atagttcttt gatatgggtt
    36121 tctcattcat ggcggaagaa gaagaataga acaaaagaat gaattaataa gatataaaaa
    36181 aagggattgt gacaaacgtc ttgtcacaat ccctttttcc aaataaacga tgttcaatca
    36241 gctgcccctc ggcattcgct cgaaaaacgg cgaaatatga agaactattt tgccatttgt
    36301 ttctctaata atcaggtcaa aacgttgact tcacagtctc ttccttaaac ggtgttctcc
    36361 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa
    36421 ttgttccttg tgactcggag ctgaacggca ggctcacgac ctctttctta aacggtgttc
    36481 aaaagcttgc atctgcggta ataagttcaa ccaaacgaaa attatgagga gctatttttg
    36541 tttggctgtt cttattactt gatgcaggac agctttttca caacctcttt tcgtttagca
    36601 ctaaatttcc ttaaaaagta gaaaagcttt cgaagcctcg tttgtttctt ttactgtata
    36661 ataaaagaaa agagtaacaa tcgctaaaag atataaacaa gagaaaggaa atacaagatg
    36721 attggtctga tttattttgt ggtgatcgtt ctggcgaaca cagttggcgc tatttccggg
    36781 atgggtggag gcgtgttgat caaaccgatt cttgatctta tcggcgccca ttctgttgca
    36841 gggatttctt tttactcaac agttgcggta ttcacgatgt ccattgtttc gactgtgcga
    36901 caagtatcaa gcgggaaatc actgaattgg cagattgtcg gttgggtatc tagtggagct
    36961 gttgttggag gagtagtcgg caacattgta ttcgaagttt ttttacagtt atttgaaaac
    37021 gagaagcatg tgcagctgat ccaaattttt ttgacggtat tgaccttggt ttttgcgttc
    37081 ttctacacca agcatcatca gccaaaattt catttgacgt catggacttg gtaccttata
    37141 tgtggggggg ttctgggctt tttagctagt tttttaggta ttggtggtgg accagtcaat
    37201 gtttctttgt tgatgctgat gtttgcattg ccaataaaag aagcaacact atattctcta
    37261 tctacgattt tcttttctca actggcaaaa ttggtaacga ttgctttaac aagtagcttt
    37321 atgcgctttg atttatctat gttgttttat gttatccccg ctgctgttat tggaggtttg
    37381 tggggagcaa gatttagcag ggttttgtct ccaaaaaaag tgacctttat ttttcaagta
    37441 attgtcctcg tggttctttt gatcaatctt tacaatgctt tcgtcatctt acaaacatta
    37501 ggagaatcta gactaacgca agtcaaaatc actgaaacat attgttctaa atgaaaaaag
    37561 gagtatactg tgataaaaaa attcatttaa aaaaataaaa attggctggt gatttggatg
    37621 ttgcgggaaa tgcgtttagg agatgcagaa gcactacgag aaatcaatgc ggaacaatta
    37681 ggatatgatg taccattaac cgttacggtg agacaaatga agaaactgtt gaaggagcca
    37741 gaaaaaaatt ttttcttggt ctatgaagat cctacttcta aaaaagcagc tggctatgta
    37801 caagcggagt cctatgaatc gattttcagc gatccgatgt tcaatattat ggcacttgct
    37861 gtgtctaaac atgctgaaaa aagaggaatc ggaaaggcct tgatgactgg cgtagaagaa
    37921 gcgaaaagac gaggaatatc agcaataaga cttaattctg cagaatacag agtagaagca
    37981 caccgatttt atgaacgcat cggttatcat tccgataaaa tgcaaaaaag atttttgaaa
    38041 atcctgtaaa tttttatcaa aaaatacctg gtcatcaagc cgatacttga tgatcaggta
    38101 ttttttttga ctaagaaagt aattgcatca agaaacctga gatgatcaaa acgatggcac
    38161 caccaagccg attgcccatt tgagcaaaag caataagttc catccgatta gctgcagata
    38221 agacagctac atttcctgta ccacccatcg agttgttgca taagccagca gtaatagttg
    38281 attcgattgg atataaacca aacaaacgac cgacaaatcc tgaaactact gaaatcgcga
    38341 taacacttgt caaacataag acaacaaatt gccatgtcaa agcagaagcc aatacattta
    38401 gattcaataa cgcaataccg ataccggcaa gaacagctgc tgtcaagttt ttgacaatca
    38461 agttgttgaa catgatagct gcatcttcgt aatattctgg taataggttt gcgattttac
    38521 agataacaac gataatgatc atgaatgcgt aagcatggac tttaggaaca aaatagttac
    38581 agatttgtcc taagatgaag aaagtcaatg ataccatcaa accgacacct aattgtggaa
    38641 tggaaggttt tacatttcta ggttttccat cgccaaggtc gcctttttcc attaatttac
    38701 cattaccgtt gaattttggc attgattgtc ccgcacgagc aactaacgct gcaccaatga
    38761 tagctaggac atttgtcatt gctgtcgcag gaatcaggcg ggaaatgata gaagaagcgt
    38821 ccgtacctaa aacatttgca taaatcgttg ataaaggtac agctccggct ccaacaccac
    38881 cagccattgc gggcaatgag atatataaaa cggaatttgc aaaaccattt ccgataagca
    38941 tacctacgag tcctacggca aaaaaggaga cgaccattga aataagtgca acgggaataa
    39001 aacgaacaga agctttcatc aatagattac ggttcatacc aaggatgcta ccagtaatca
    39061 aggcagcaat ataaaagtct aaaaagccca tattgctgac aaagttttct gtggatttta
    39121 ctacatactc aggtaagatc ccaaatgtag caatcgcggc agaggcaaag atcgcaaaaa
    39181 cggagccacc acctaaataa cttttgacga tagggatttt tgttccgata aaatgaaata
    39241 aattaccgaa aatgaccaaa acagcgattg ggccaatcat attcgtcggt aattttccta
    39301 aagccattgc aatgatcagg acaaggacca tgatcaaata aaggggcaaa cttacgccgg
    39361 taatttttgt tgaccaaaac ccttcttttt tcttttcctc aactgcagtt gcggttgttg
    39421 ttttttgtgc catttttaca gatcctttct tattctgtat cgtatgaaaa aagccaaaga
    39481 gaagcggcaa aagagcagta gttggctggc tgctgttttg ccgcagtcgc ttttttcttt
    39541 ttagacgtca tacaaagttt ctgtatgttc ttttactata acaatcttaa tcttctaaag
    39601 atttatattc aggaacccat ttagcagatt caacagcttc tttgatatcc gtgatttctt
    39661 cacgattcaa tttttgtgca acaactgatt ttgcgacagt ttcagcaatc gtttgagaga
    39721 attcagtgat ttttgctact ggaggcaaga tagctgctcc tggttttgtt acatcaacga
    39781 ttccgcctaa tgctcgacta gcttgagaaa tgatttcagc atttactcgt gtagctgttg
    39841 aagcaatcaa accaagccct aaacctgggt acatcaacgc attatttgct tgaccgattt
    39901 ggtaagttac accgttatat tccacatcag cagcagggat acctgttcca actaaagctt
    39961 ttccgtccgt ccattcaatc aaatcttttg cttttgcttc tgccaatttt gttggattag
    40021 acaaaggcat gatgattgga cgtggggtat gagccgccat ttctttcacg atttcttcag
    40081 tgaatgctcc aggttgggta gaagtaccga tcatgatcgt tggatggatt gcttttacaa
    40141 ctgcttctaa gtttgtcaat tcatcagcat tagtgaattc tgagcgttta cgagcgaatg
    40201 ggatttgacc aggagtcaat ccttttgtat cttcaaacaa taaaccctgt ttatctactt
    40261 gatagaaatg ttggcgtgct tcttcttcag gtacaccttg acggatcatt tcatctaaaa
    40321 ggatgtttgc gatacctaca ccagcagtac cagcaccaaa tgtcaagatc gtttgatctt
    40381 tcaattgctc acctgatata ttcaatccac ctaatacacc agctagtaca acgattcctg
    40441 tgccttggat atcatcattg aaagtagtga ttttatcttc gtatttttct aagatcgttg
    40501 cagcatttcc tcgtccaaaa tcttcccaat gtaataacaa ttctgggaat aattcagtag
    40561 ctgattgaac gaattgatca atgaattcgt agtaagtttc gccttctaca cgagcatgac
    40621 gattccctaa gtataatgga ttattcaata attcttcgtt attagtacca gcgtcgattg
    40681 atacaggcaa gacttgtgca ggattgatac cagccgcagc agtgtagacc atcaatttac
    40741 cgatagcgat gtcaacaccg ttcacacccc agtcacccat acctaagatt ccttctgcat
    40801 ctgtcacaac gatcaaacga atgtcgcgtc catctgctgc gtttttcaaa cttgctttga
    40861 tattttctgg ttcgtcgatt gaaagaaatg cagcatcttg tggtttcaag aaaatttcgt
    40921 tgtattgttc gattgaatcc gcaacgacag gatcataaac gacaggcatg aattcaacta
    40981 aatgttcgcc catcaattta taaaacaatg tccggttcgt attgaataga ttcattaaga
    41041 agatacgttt ttccaaatcg gtctgtttgc ttaagtattg accgtaagct tggacagatt
    41101 gttgttccaa tgtttggaca gtacttggaa gcataccagt gattccgtat tttgcccgtt
    41161 cttcttttgt aaaagctgtt cctttattta gaaaaggatt atttaataag tttagccctg
    41221 taagcatgaa tgattcctcc taagttttaa tacaaacgaa gtataggagt cagatgagga
    41281 tatagtcaaa aagagcaggt ataataaatt ttatttatat gagtaataga gtcaaaagaa
    41341 atcaaaatag cggtatttgt tctttttata tcaacaatcc ttgcttgatt actatgcgta
    41401 tctcttatat gctaaagaaa gaaaagtaga agaataaagg atatgttaga tagataaata
    41461 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
    41521 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
    41581 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
    41641 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
    41701 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
    41761 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
    41821 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
    41881 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
    41941 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
    42001 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
    42061 gacacgactt ttgtcaaagt cccatcgttt tttatctatt tgtgttctaa acatttcttt
    42121 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
    42181 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
    42241 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
    42301 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
    42361 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
    42421 atcagctgat tattcctcat gtattaaaag atgtagaatt acccgataca actacacatt
    42481 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
    42541 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
    42601 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
    42661 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
    42721 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
    42781 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
    42841 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
    42901 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
    42961 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
    43021 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
    43081 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
    43141 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
    43201 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
    43261 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
    43321 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
    43381 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
    43441 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact taataaaaaa ggaagaatat
    43501 tcatgtaaac gattttattg tttttttctc ttttatacaa aaagggtcat aatttcaagt
    43561 aagcggatga attcatgagg attttttgca tactgtctct actatactta ggtcactaag
    43621 aaaaaggagt gatttattat gaaaaagaac atgattggga cattattact ttcaacactt
    43681 cttctaggcg gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat
    43741 attaccttta ctgaaccaac aaataccgtg gagcctctta accctactga tccttcaaaa
    43801 ccagtagagc cagcagatcc ggaaaatcca gcaactggcc agacaggtcc tttaacactg
    43861 gatgtagttc ccgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat
    43921 caagtagacg cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta
    43981 ggtgcagatg gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac
    44041 ggttcacaag ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg
    44101 tcagataacg aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt
    44161 gcagcgacac caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt
    44221 tatgatccag caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct
    44281 gatttgactt ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa
    44341 actgcggtga aaatactata ggtgaaggat tttttaccgc agtttttgtt gaaaggatgt
    44401 ggtggacaat gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat
    44461 ttttcatttt tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac
    44521 cggtatttcc agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac
    44581 cggaacaaaa tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat
    44641 ttgaggtctc tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa
    44701 aaaacccaac gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga
    44761 aaaaggaaat caatgtatca gcacatgcag aaacaactgt acctatagaa gtgaaaatcc
    44821 cagcaaaaag ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag
    44881 ctgaaacaga aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg
    44941 cagtcgtttt acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag
    45001 atttggacga ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta
    45061 ggattatcag caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg
    45121 aaaatacgtc taatgcattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt
    45181 tagctggtga taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg
    45241 acagtaatga atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag
    45301 taaatgaaaa ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat
    45361 tggttgctgc agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa
    45421 agaaaaaagg agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga
    45481 gggattattg caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa
    45541 agttcaggta cgattacatt ttatgatcta agtccaattc cgcatccaag tggttcagtc
    45601 aatggcgcat caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc
    45661 gtattcccgg agacgaatga acaaaagatc cgttttcttt atttgatcat tggtatagaa
    45721 ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt
    45781 gctacaaaat aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag
    45841 tatatttatc ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta
    45901 tgatccatac acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg
    45961 cgggaagctt gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt
    46021 gaacgatggt tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca
    46081 tgtgaaacta acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt
    46141 ggcgatcatc aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg
    46201 gaacaacgcc tacttgtata ttgccggtgc ccccatcaag gttactagcg gaagtttatt
    46261 atcagcagga agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt
    46321 gacagatatt attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca
    46381 atttgatgtt acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag
    46441 tccaaacaaa attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt
    46501 ttctgttgat ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt
    46561 agctccagct gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc
    46621 atatgcattt tctgcaagtg cagagataaa tacgtggaat agttacatct caccaagtga
    46681 ttcaacagga aaatataata catttgctga taccacagaa tacgtggatg gaaataacca
    46741 gaaccttcca ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt
    46801 tgatcagttg ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt
    46861 tacacaagga gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc
    46921 caatatcgtg tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa
    46981 ggtattggag cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag
    47041 tcagtgggga aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac
    47101 tgaactaaaa aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc
    47161 gacaggatgg acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac
    47221 agctacttct acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt
    47281 ggacgatgta cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc
    47341 tggaatcacc actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag
    47401 tcagctaaaa aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg
    47461 agatacgatt caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga
    47521 caaaaaaatc acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt
    47581 aaatggtaca actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct
    47641 attgagcaaa ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga
    47701 tacttcttta caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac
    47761 attagcagat ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct
    47821 tgtccccaaa caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga
    47881 tctccccaaa ccgttgaaga caagttatta tgaatcaaaa ggcgattttt cttttgatgt
    47941 gcgggatact cggcttccaa gcacgtctcc ttggcaactg acaggtacat tgacttcttt
    48001 atttaagaat gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg
    48061 atcaaagcaa ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc
    48121 taaaggagag gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag
    48181 cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg gtcacatggg aactgacagc
    48241 tgggccaacc agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct
    48301 ctttttcaag gatgagaagg aaacttctgc tgttaaaatc agaaatgctt gaaggagtgg
    48361 acttggaact tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac
    48421 ttttattaga ggaaaaagga gaggtttccg ttaataacgt acttacccgc ttatcagttg
    48481 atcgggctac cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac
    48541 aattggaaat tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag
    48601 cagtcgatgt ttattatcat tgttacttgc gctattctac aaaatatcag atacttcttt
    48661 atctgttgga aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt
    48721 cggctacttt gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga
    48781 aaataaaaag cgggcggctg accggtccag aaagtcaaat tcgttatttt tatttccaat
    48841 tattttggtt tggacgtccc tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa
    48901 acactttttt agatctgctg caaaaagagt ttagttttgc tattacagaa gaaggacgat
    48961 tgaaatttga aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca
    49021 aaccagatta tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc
    49081 tccatacatt tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa
    49141 cgttggtttt ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg
    49201 tccaggagtt tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa
    49261 cgtttaaaca atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc
    49321 aaaagattgc gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga
    49381 tgaatatttt tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat
    49441 tcctggtttt atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag
    49501 tagacgagga aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttacggacgc
    49561 tgatcaagca gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa
    49621 tggcggatat cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt
    49681 tagtcgaata cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt
    49741 acccgtcaca gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt
    49801 tcccctattt gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga
    49861 ataaaacatg aaaatcgtga tcattggtgc ttcccatgaa ggcatcacag ctgccctaaa
    49921 tctaagaaaa cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg
    49981 actaggctat gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc
    50041 tgaagttgcc cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg
    50101 gagagtaact gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa
    50161 aaacgagacc ataacctatg ataaattaat cgtagcgaca ggttcctctc ctgtcacctt
    50221 gtataaacaa attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca
    50281 agtacttgcc gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttacattgg
    50341 attggaatta gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat
    50401 gccgaatgtt ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct
    50461 gcaaactaag caaataaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga
    50521 agagaaagta gtatccgtac aacttctgtc tcaaacaata aaggcagata tggtcatttt
    50581 ttctgcccaa actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga
    50641 tgagactgtg atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg
    50701 agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac
    50761 tcgagctgta catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta
    50821 cgacctcaga caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg
    50881 gctgacagaa gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt
    50941 tgatctattt ccacaatacg acgaagaaaa caaaacagta aatgcaaagc tgatctacca
    51001 tccggataca ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga
    51061 tttgaacctg cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga
    51121 ggattttgga tttctgtctg aatacacctc tcgttttcac tacctcaatg agttagcttt
    51181 taaagtacta gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc
    51241 acggtctttt attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat
    51301 ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat
    51361 aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat
    51421 gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc
    51481 tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca
    51541 gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg
    51601 atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt
    51661 gcccaaacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt
    51721 agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct
    51781 tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc
    51841 ataggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg
    51901 gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagaa
    51961 atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga
    52021 ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat
    52081 gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc
    52141 gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat
    52201 atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg
    52261 gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga
    52321 acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc
    52381 attagcttgt tgatgttagt agtagctgtg ttaggcgcac tattttctgt tcggacaatc
    52441 gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagaacatg aaagcaatag
    52501 aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag
    52561 aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag
    52621 gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat
    52681 ttatcaatga tcaagaattc agtaatcaaa aagaaagcga acgcgttaaa ctacgtttta
    52741 aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc
    52801 agttattatt agtagataag attaaacatg aacatcgtca aaaagaagca aacgaattat
    52861 ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag
    52921 aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag
    52981 atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa
    53041 aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg
    53101 acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa
    53161 aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa
    53221 gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag agaaatcgca
    53281 caaaaagttg ggatcaccca gcccaatctc tatcattatt tcggtgataa agaaaaacta
    53341 tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt
    53401 caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag
    53461 acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa
    53521 acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc
    53581 ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat
    53641 tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta
    53701 aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt
    53761 tatataggga gaaaatagtt aggtgaagaa gcaagatatg acgaagatcg ttatatcttg
    53821 cttctttata taggtagttt ttcataaata caaatttgtt attctctcat gattatcagg
    53881 gtatattttt atttattatg tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa
    53941 ttaattgttt gtagaatgaa ggaaactatt tattgggtag atgaatcacc acgaatagtc
    54001 aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca ttttagcagt aatgttattt
    54061 tctacggtta tatcttttac agcccctttt ttgcttactt aagctgattt taagttttgg
    54121 cccttagtct ggatctacga ggtgagagtc aagaatgaaa aaaataattg gagcttttgt
    54181 atttgcaatc attatcatta ttatctgttt tcaaatagat gtttttggtc aaaaaaagat
    54241 agttggacaa acggtcagca gtacgaacag tacggttaca aagacaaagg aatcatttga
    54301 tgatacagaa caagagacat taatcaaaat cggaaaaaat gaccgtacac tatatacaga
    54361 tgactcgcta actaccccac ttgcaactat aaacggcgga gagctggcgg agtttctttc
    54421 tgaaacaaaa aatgcttatc agatcaagac aaatgatggc tatactggat atttagccct
    54481 cgaagatgga accaaagtca caaagaatat acaaacaaaa ccggaagagc tatctgatgc
    54541 agtgattgta ttagatcctg ggcatggcgg aaatgatact ggggcattaa gcaatgatga
    54601 acaaacagaa gaaaaagata taacattaag tacagccata aaagtaaaaa aggcgctaga
    54661 ggatgctgga gcaacggttt atttgaccca tactacagat gaattagtac agcttggaga
    54721 tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt atcagtttac acgcagactc
    54781 tacggaatat gcgaatgaag caactggaat cacgacatat tattactatg gacaagagga
    54841 aacattagca cagacaattg ctgactcctt cactgatctg cctctggatt caagaggaat
    54901 ctccactggg aactatcaag ttcttcgaga aaatcttcaa ccttctattt taatcgagat
    54961 gggttatatg aataatgata gtgatttaaa ggaattagta acagacagtt atcagcagca
    55021 gattgctgca agtttgacac aagcattgac cacttatttt cagtaataaa taagcccagc
    55081 caaacaaaaa tagctcctca tatttttcgt ttggccgggc ttattatcgc agatgcaagc
    55141 ttttaaacac cgtttaagga agaggtcgtg agcctgccgt tcaactctga gtcacaagga
    55201 gcaatttcaa aaaacagctt ctcatgtttg atgactattg tgacttgtga ccgaggagtt
    55261 ggcatgagaa caccgtttaa gaaaaaggtt gtgaaatcaa cgttttgttc tgagtattag
    55321 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa taccgaggat
    55381 cagatgattg aacaccgttt attcggaaca agggactgtg acatgacttt tgtcacagtt
    55441 ccattatttt tttagtagct tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata
    55501 tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt ggtcagattt
    55561 cgttccagct cctgacggaa gggtgattcc agcttcagct gatgctttgt ctaactcttc
    55621 caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat attgttctcc
    55681 tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg catatttttt
    55741 aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt ctggctcgat
    55801 tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt
    55861 gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt tataggggat
    55921 ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact cttttgaatc
    55981 tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag cagcacatac
    56041 tgttagcgga ccgaaatagc tgccatttcc gacctcatca ctacctaaaa cggataattg
    56101 actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc
    56161 ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca cttttccgga
    56221 tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat aaggaatctg
    56281 tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt cgtttgttgc
    56341 tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac tataccgcaa
    56401 ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa agtttatcat
    56461 ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca acttcatggt
    56521 acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca cgaaaaaaca
    56581 cgctacaaag cggtgattgc caaccaaaca tatactatta tcggacggga aaccaagcat
    56641 catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa acaattatca
    56701 ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa
    56761 ttgaagaaac aagaaagaat attggagctg gaagaagaaa cagcagaatt aaagaaaaaa
    56821 atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa
    56881 gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa
    56941 gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa
    57001 gatcctttta tcaaaataag aaaaatttaa aaaagttaga agagaaagga acgtagcatg
    57061 ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc acgtcgcggg
    57121 tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc tgcccagcat
    57181 ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc tgtcacccca
    57241 acatcaaagt tagcattttt tgatcaggta tttgcctttc acctagatga agctttttat
    57301 gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg atttgtgggg
    57361 gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg gattgcagga
    57421 ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca gttacttgcc
    57481 tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc tcgttttatc
    57541 gttgagagaa cgccatttct taccaacaag atgtatgagc tttgggtaac gaatattttg
    57601 aattaaggaa aatagtgctg gaaaatattt cgttttttgt acagagtagt ttatttaaga
    57661 aggctgcgta gcgtatgtcc ggcacagctc ttttttcata gtgaaagata aggtgataaa
    57721 atgaataacc gaatccttga aacattggaa tttgaaaaag tcaaacagat ggtcagacaa
    57781 tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa
    57841 caaacgatca caaactggct tcaagaaact gaggatggat tgaaagtgca acgtctgcga
    57901 ggcgggatcc ctatacctaa aattgaaaat atccgtcccc atatgaaacg gattgagatc
    57961 ggagcagact tgaatggagt ggagcttgca caagtttcca gagtattgtc tacgacaagc
    58021 gaattgaatc gtttcattga tgatctttct gacagtgaaa tcgaatttgc acgtttgtat
    58081 atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa ggaagcaatc
    58141 gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat aagacagaac
    58201 attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat
    58261 gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt tatcccagtc
    58321 aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc ttcaggtcag
    58381 actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg tcaataccaa
    58441 atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga acttgttccc
    58501 catcgtcaag aaatcatcca caatacttat gtgattggta aaatggatct gatgaatgca
    58561 aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga agacaatcat
    58621 gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt ttctaatgac
    58681 atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac aggtgggaaa
    58741 acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg attaccgatt
    58801 cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga tataggagac
    58861 gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa cactgtagat
    58921 attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg tgcaggaaca
    58981 gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg taaaaagtct
    59041 gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata taatcgggca
    59101 aatacgatta atgccagcat ggagtttgat gtggatacat taagtccaac gtatcgtttg
    59161 ctgattggcg ttcctggacg aagcaatgcg tttgaaatct ctagccgatt aggtcttgat
    59221 acagaagtta ttgatgaagc gaagcagttg atgaatgatg agagccaaga tttaaacgaa
    59281 atgatcacag atttagagaa tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt
    59341 cattttgttt ctgaggcaca agaacttcac gatgatttga aagaagctta cagctatttc
    59401 tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaatga agtcgtgtca
    59461 gaggcggaag aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca acagcagatt
    59521 gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa agacccaatt agcaaatcta
    59581 catcaagaag aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg
    59641 ttgaaaccgg gagacgaagt gttggttacg acttacggcc aaagagggac gcttttacgt
    59701 aaaaacggga atcagtggca agtagaaatc ggtatattaa aaatgaatgt gtccgaagac
    59761 gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac gagttatcca tgctgtacga
    59821 tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa
    59881 gcgctaagtg aggtagatca atatttggat tctgccattc tagctggtta tcctcaagtg
    59941 actattgtcc acggaaaagg aacaggcgca cttcgcaagg gaattactga ttatcttaaa
    60001 aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg
    60061 acaattgtta aatttaagta aagcttacta aaggtaagca gatggttcgc tatccaagta
    60121 tagaaatgct atactagtca tagattttaa aaatggaggt ctttgatatg tcacaagtaa
    60181 ttacagataa gacatttaat gaagaaacag ataaaggact tgtattgatt gatttttggg
    60241 caacttggtg tggtccatgt cgtatgcaag caccgatttt ggatcagttg gaacaagaat
    60301 atgatgaaga agaattcaga attgctaaaa tggatgtgga tgaaaatcca gaaactcctc
    60361 aacaattcgg tatcatgagc attccaacat taatgttgaa aaaagatggt caagtagtag
    60421 aaaaagctgt tggtgttcat tcaaaagaac aactacgtca aatgatcgat caatatttat
    60481 aaaaacttac agcaaaaata agctgagggt gggactgaat ccagttccgt cctcggtttt
    60541 tttgcggttg agttaaggtt attcaaatta atattttttg agtataatta cgttgtcagg
    60601 ctggattgtt tttttgacag acatgctata ataactttaa ttgaatatga aggggagtac
    60661 cagatgaacg aacgaatcaa aaacaaatta gctttacttc ctgatcagcc cggctgttac
    60721 ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca aagccaaaat attaaaaaat
    60781 cgtgtccgct catattttac tggtagccat aatacgaaaa cggaacggct tgttagtgaa
    60841 atcgaagact ttgaatacat cgttacagaa tcgaatatcg aagcattgct cttagagatc
    60901 aatctgataa agaaaaatga tcctaaatac aacattatgt taaaggatga taaaacctat
    60961 cctttcctta agataacgaa tgaaaaatat ccccgtttgg tgattactcg taaggtacta
    61021 aaagacaaag cactgtattt tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag
    61081 aagatcctcg atcgattatt tcctctgaga aaatgcaaac ctagtcagac gaaggagcct
    61141 tgtctttact atcatttggg acaatgcctg tgtccttatt atttcgacgt agatccgaaa
    61201 gtctatacga aaatcgttga tgaagtgaaa aactttttaa acggaggaca cgaaaaaatc
    61261 gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct
    61321 gcagaatacc gagaccagat ccgtgccatt gagacaatca tgactcgcca aaaaatgaca
    61381 aatactgatt tactggatcg agatgtattt ggttatgcga ttgataaagg atggatgtgt
    61441 gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac gagatgtttc aatgtttcct
    61501 ttctataacg aagcagaaga agatttcttg acttacctcg gtcagtttta tcaggaaaat
    61561 gagcatttca ttcctaaaga agtcttgatt cctgatacga tcgacaagcc cagtgtagaa
    61621 gctttgttag ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa attagttgaa
    61681 ttggcgagta agaatgcctc agttgctttg aatgaacggt ttgatctgat tgccagaaaa
    61741 caggaacgga cgatcggagc agttgaacgt ttaggaaacg cgatgaatat cccgacccca
    61801 atacgtatcg agtcttttga taactctaat atcatgggga cgaatcctgt ggctgcgatg
    61861 gtcgtattca ttgatggaaa accggcaaga aaagaatatc ggaaatataa aataaaaaca
    61921 gtcaacggac cggatgatta tgcatcaatg agagaagtca tctatcgcag atattcgcga
    61981 gtgattcgcg aagggttgcc tttacctgat ttgatcttga tcgatggagg aaaaggacaa
    62041 gtagatgtgg caaaagatgt cttagaaaat caattaggaa tcgatattcc tattgctggc
    62101 atggtaaaaa acgataaaca taaaacaaat gagttgctat tcggtacaga tttggctgta
    62161 gtaccgcttg agcgaaattc accagagttt ttcttactcc agcgaatcca agatgaagtc
    62221 catcgattcg caatcacttt ccatcggcag ctacgcagta aaaacagttt tgcttcccga
    62281 ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa
    62341 tcgttgaaaa atatcacagc agccacgaca gaagaattac aagaaatcgg actgccgaaa
    62401 aatgtcgcac aaaacgtatt tgataaatta catcaggaat gaggaaaggg cactttacaa
    62461 agaaagtaaa gtgtcctatc attttatgta caacaaaaag agcgtttacg ttttgttggt
    62521 aaataataaa tcgcttacgt ctggtgtcta aggatatatg tgatttcttg aagaaggagt
    62581 gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg ggaactacac agctgtcgat
    62641 catgtctctt tccaaatccc cgatggaaaa atcatggggc taattggcca aaacggggct
    62701 gggaagacaa cgacttttcg tttgatcttg gattttttga cacaagacca aggagaagtg
    62761 ttatggaatg gccatcatct tggggagaaa gattatgatg tcatcggtta tcttccggag
    62821 gaacggggac tttatccgaa agtaacgatt caagaccaac tgctttattt tgcagaactt
    62881 agagggaaat caagaaaaga gattgaacca aaaatcgact tttggatgga aaaatttcag
    62941 gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc
    63001 cagctgatcg ccacgttaat ccatgaacct aaattgatca ttttggatga gccattcagc
    63061 gggttagatc cggtaaatgc tgaattgcta aaagatggaa tattggaatt gaaaaaacat
    63121 ggttcatgtg tcattttttc tagccacaat atggataatg tagaaaaaat ctgcgatcat
    63181 ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa
    63241 tcatttggcc gtacgaagtt attcttggaa tctggtttaa gccaacagga agtagaagaa
    63301 atcgaaggcg tgaaaaaagt gatccttcat gaagacaata cgttagagat taccttagaa
    63361 gatcctgaag ctgggaaaga aatctttact cgtgcgactc agttcggcta cattcctatg
    63421 tttaaccagc aaccgccaac tttggaagag attttcaaat tgaaagcagg tgaaatcgat
    63481 gcgtaagttt tggattatta ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt
    63541 cttgatcatg atattagtcc cttttatcat gctgggtatc atttatgtag caggaaactt
    63601 tgctcaacaa aatagtgatg tcaataaaat cggcgtattg gctgataatg agcaaatcag
    63661 tcagcaaatg agccaagtca aaacagatga ttttgagttc aaagcagttg attctgaaga
    63721 aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg tatttagtag tctcaactga
    63781 gaatgataag ataaatggga tgctttatag cgaaaattca ttaggacaaa gtacacaact
    63841 actgatacaa caacaactga ctggcttgca atcgatgctt cgagcgagtc aattaggcat
    63901 ttcaccagaa gaagtagcta gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa
    63961 ttttgattca aaaggaaaaa tgacagtcgg agaagataac tcgacagtcc aatacgtcgt
    64021 aagttatgta gcaacgattg tcttgttcat tattattttg acgtatgcgc agatcattgc
    64081 acaagagatc gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac
    64141 tgcacaaaag catttttacg gaaaattaac aggtgtttta cttgttgcat taacgcaaat
    64201 ggcattatac ggtattattt ttggtatagg atacagtcaa ttcaaagata tggaaatggt
    64261 caaaaatata ttagacggta tctcattgga taacttgatt ggaccattct tgtggtattc
    64321 tcttctattc atgatctttg gcattttgat tttttctgtt ttagcagctt tatgtggctc
    64381 tttggtcaac aaagcagaag atacagcaaa agcaattttg ccagtgactt atctatctct
    64441 aggcggatac atgcttggat tgatactagg agcttcagat ccaaacaata tcgttattcg
    64501 gatcacttcg tatattccat tcttatcttc atatattatg ccgattcgct tagcaaatga
    64561 gacagtcggt atgggtggag cattgatttc cttgttggta cttgtagccg taacattctt
    64621 attgatgatc ttgtctgcaa acatgtacaa atcgaatgta ctcgtttata gcgaaggcgg
    64681 attatggagt tcattgaaac aatcgatttc aattatgagg aatgaacgaa aaaaagcata
    64741 aaacaatcct aaaatacaga aggaagctgg acatctattg ttcccagctt ctttttgtat
    64801 agacctattt tatttatttt actatattgg atatatattc attgaaaata gtgtataaca
    64861 actttataat attgagtata ccaatttttg gaggcgtttt aatggaccgt tatttagatt
    64921 tactttcaga aaaattcccc tcaacggagg atgtcatcac ggaaatcatc aatctcgaag
    64981 caattttgca gcttccaaaa ggaacagagt tatttttgag tgatatacat ggcgaatttc
    65041 ctgcttttga tcatattctg cgtatcggtt caggaaatct taaagaaaag atcaaagacc
    65101 ttttttctga acaaatgacg gaaacggata tcagccaatt cactattttt acggcttatc
    65161 cagaatatgc attaacgact gattggtaca aagaacaaga taaatcgcag ctgatccatc
    65221 atttgatcga tcttttacgg tttacgacag tcaaatatac ccggtcaaaa gttcgaaaag
    65281 gattgccgaa agaatatagc tatatcattg aggaactctt gtatatcgat gaccgtatca
    65341 atggaaaaaa agactatgta aaaaaaatca ttgaacaact tgttttgatg aaggaagaag
    65401 aaaaattttt aacgaaactt gcacagacga tccaaaaatc tgtcatcgat catttgcata
    65461 tcgttggaga tatttttgac cgaggaactc aagcagccaa agtgatggat cgaatcatgg
    65521 atttttcttc tgtagatatc caatggggaa atcatgatat tttgtggatt ggtgcgtatt
    65581 gcggctctga ggcatgttta ctgacacttt tacggattgc agcacgctat aattatttat
    65641 ttgaattaga gaaagaatat gggttgaatt tacgtccatt gttttctttt gctcatcaaa
    65701 cttatcagaa gaatccagta tttgcgccta aaaacagtaa aaatctatcc gaaagagagc
    65761 aagaagaatt agaaaaaatc catcaggcac tttctatcct tcaattcaaa tcagaacatc
    65821 agttattgga tcgtcgacct gaatttaaaa tggacgatcg taaattgctg gaaaaaatcg
    65881 actatgaagc atcaacgatt gatttagaag gtcagctcta tgagttgaaa ggaacttgtt
    65941 ttcaaacgat ccagccagat gatccaaaaa aattcttgga agaggaacaa aaagtgattg
    66001 atagtttgat gtattcattc cagcactctt tgagaatgca aaaacatatg acgtttttga
    66061 tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt gctgtttcat ggctgtatac
    66121 ctgtagacga gaagggacag ctcttagcat ttgaattaga ccaatcttat cgcggaaaag
    66181 aactgctggg cttttttgaa aaacagataa ctgaaagcgc aaaagatctg actgcaaaag
    66241 aagatttagc aaccgatttg atttggtatg cgtggagtgg gccattttcg cctttattcg
    66301 gcaagagtaa aatggcgact tttgaacgtt attttctcac agagcctcat acgcacgtcg
    66361 agaaaagcaa tccttattat cacttgcgag atgaagagtg gttcagtgaa aaaattttag
    66421 cagaattcga cacaaaagaa gaagggtcag caatcattaa tgggcacacg ccagtaaaag
    66481 taaaaaaagg ggagtcaccg atcaaagcaa atggaaaagt atttgtcatt gacggaggtt
    66541 tatccaaagc ttatcaaaaa acgacaggaa ttgccggtta ttcactatta tgcaattctt
    66601 atggtttcca aatcgtgaca cattatccgt ttttgcctat tgaacaacaa tttgcaaaaa
    66661 aatccgacca aacaaatgtg aaaaaagtaa tcgaacagca actaccacgc aaactcaatc
    66721 gtgatacaac aaaaggtatg gaactgcagc agcagattac ccagctcaaa aggctgttgg
    66781 actatcgaaa ggacgattga gaagaattcc agtgaacatt gttccataga ctggagactg
    66841 agacaaaagt cgtgtcgcag tctctttttc cggataaacg gtgttcttct gccaactcct
    66901 cggtcacaag tcacactatt caacaaacat gaaaagctgt tttttgaaat tgctccttgt
    66961 gactcagagt tgaacggcag gctcacgacc tcttggtata aacggtgttc aaaagccagt
    67021 ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag ccattttcac aaaatttttc
    67081 ttaatactcg aaactaaaca gctttttcac aaccccctca gaaagtctat taaaattcat
    67141 tcacaaagaa atcgaataag cgctgttcag atggatgtac acggtgggta agttccggat
    67201 gccattgaac accgaggatt ttatgcatat catctttgct ttgaaccgct tcaacgagtc
    67261 catcgttaga aaaagcgatg gcttttaata aaggtgaaag atctttgact gcctgatgat
    67321 ggtaagaatt cacaaaatat ggatcgggga gaaggttgct taacaaactg tctgaaacaa
    67381 tcttgacttc gtgagtcgta aattgcgggg cagtgggctg ttgttcatgt tttactgacc
    67441 actttggata taatgaaaga tcttgataaa gtgtaccttc caaaacgaca ttcaatagtt
    67501 ggagtccccg gcagactgca aaaattggtt ttctttgttt caatgcttcc aagatcagtg
    67561 cctgttcaaa ttggtctcgt tgaatatttg tttccgttag ctttggatga ggatcttcca
    67621 tgtaaagttg tggactgaca tcttgaccac ctgcaagaat aagtttgtcg atttgtccaa
    67681 tcagttgagg agctgaatct ggagacatga ctggcagaat aagaggcaat ccttgagcat
    67741 ctaaaactgc atccacgaat ccttgcggtg tatagctcac ttgatttcct tggaaagtat
    67801 ctgttgctcg aatcagttga tttccggcaa tgccgatgat tggtttcatt tgtattcccc
    67861 ctaaagtaga cttattgtaa caaggattta aaaaaaatta aactatgtaa gacttgcccc
    67921 tatacttatc tgttcttgct gtgttactat ctgtaaagag aaatttcgtg tgaaattcat
    67981 gacgatagtt gaaaatattg cttaatttgt tataataaaa agtaagcaga aataaaaagg
    68041 ttgggcgtaa gcttcaagga tctagttctt caaggggtga gaaggacatt ttattgaagg
    68101 ggcctgccta tactaagtta tctttagagg tacatacttt caatgaaaaa agaaagcatg
    68161 tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta gaagctttgg aacaaatccg
    68221 aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg cggcagcagc tgaagatcta
    68281 tgcaacagaa aaagagcagg aattggaaac gatccaaaca aactatcgac aacaactaaa
    68341 acagttggtg gaagaaaaag aacaatttga aatacaagaa ttagaaaaag aaaaaatgaa
    68401 attgaaagca gaagcagaca aggtgcaagc aactttgaaa gatcaatatc aaaagcatca
    68461 gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa acttatggcc gtcactaaga
    68521 tggaaaaagt gaccctcatt tcagataaaa gaaatcagga gaggatttta caagcagtac
    68581 aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa tagaacaaac aaccagtggg
    68641 tggagaagta ttttccagat accacgatta ttgataaaga agcaaaatta agcgctttag
    68701 gtaatcggtt gaaagacatc caagaagcta tccaattcat acaacaccac ggcgatagcc
    68761 gtcaaaagaa actccatttg aaacgtcaag aactttcctt acaagatttg gaagccaatt
    68821 attccgaaga aaatttcata aataaattga aagaagtcct tgatttaaaa gaaaagtggg
    68881 aacaactaac tgaagaacgt gatcgtttag aagaagagga agaatggctt cttcattggc
    68941 aacagctgga tattgctcca agtatgtatg caagtaagac gaccattttt gagatgggga
    69001 ctgtgagtgc agcaaatttt gaaagtttca aggaagaact gagtaagctt gatgacgttt
    69061 atatggaaga aatagattat acgccaaaac aagtttatct ttcgtatgtt gctttaaaaa
    69121 aatcagttgc tgttatagaa gaaattgctg ggaaatacgg gttcaataaa gaaacgtatc
    69181 cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa gcagcaatta tccattgttc
    69241 acgagcagca aaagaagata acgacagcgt taggtcagtg cagtggctat atccaagaat
    69301 ttgaatgggt agaagaagtc acacttgccg ttgcagaacg agaaaaaata aaagaacggt
    69361 ttatccatgc gacttatctg atcgttttgc aaggttgggt agatgcagaa gaaaaacaga
    69421 atttgctgca tgtgttaaac gaaaccgtat cagaagaaga tatatacgtc agtttcgaaa
    69481 caccaaactc agaagagatt cagcaggaag tccctactaa attgaaaaac catccgcttg
    69541 tcgaaccttt tgaattgctg actgaaatgt acagtctgcc aaaatatgaa gaagtagatc
    69601 caacaccttg gatgacacca ttttatcttg tcttcttcgg gatgatggtc gcagatgttg
    69661 gttatggttt gctgatgctc attgggacaa tactagctca aaaactactt gttctaccac
    69721 gcgggatgaa acgtttcgta aaattctttg agattctctc gataccatcg atcatctggg
    69781 gattgatcta cagttcattt ttcggacagt cattaccaaa agaactgttt ggcatccgct
    69841 tgcctttccc aatcctatct acgacggatg acgtcaatac gattttgatt ttatccgtga
    69901 ttttcggtct gatacagatt ttggttggat tatttgtggc agcaaaggaa aatatcagga
    69961 gaaaagatta cttgagtgct gtcagtgatg gatttgcctg gcaagggatc ctaattggga
    70021 tcgttattgc gttggttggt gccatgttga taaaaaatac agcatttgtt taccttggcg
    70081 ctgggctatc gatcattgct gccttatgta tcttgatcgt tccgattatc caaacgccgt
    70141 ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta cggactgacc ggctatatcg
    70201 gtgatttagt cagttataca cgtttgatgg cattgggtat ttcaggcgga agtattggag
    70261 cggcattcaa tatgctagtt gctttcatgc cgccagctgc tcgattcagt gtagggatcc
    70321 ttttgatcat cgctttacac gcactgaata tgtttttgac actgctaagt gcctatgtcc
    70381 atggcgcacg attgcagtat gtcgaatttt ttgggaagtt ttatacagga ggcggacgtg
    70441 cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat caatcacaag aaaaaaaaca
    70501 ataaataatt ggaggatttt tatataatga tggattactt aattagtcaa aatggtggaa
    70561 tggtatttgc ggtattagcg atggcaacag cgacgatttt ttcagggatc ggttcagcta
    70621 aaggtgttgg aatgacaggt gaggcagcag cagcattgac aacaagtcaa cccgaaaaat
    70681 tcgggcaggc tctgattctg caattgcttc caggcacgca aggtttgtat ggattcgtta
    70741 ttgccttttt gatttttatc aacttaggaa atgacatgtc agttgtacaa ggactgaact
    70801 ttctaggtgc ttctctgccg attgctttta caggcttgtt ttcaggtatt gcccaaggaa
    70861 aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc tgaacatgct acaaaaggaa
    70921 tcatttttgc tgcgatggtt gaaacatacg cgattttagg tttcgttatt tccttcctat
    70981 tagtgttgaa tgcctaagca taaaggagga aaagcagatg aacgccattg aaaaaatcat
    71041 ctctcagatg aacgaagcag cagagcagga aagagcggcg ttagaacaag aagagcgaat
    71101 gaagatcgat caaaactttg aacaaaaacg tacgcaagtt gaaacagaac atcaaaaaca
    71161 aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt cagctaagga atcgccagca
    71221 agtagaagta cgacaagaaa atttgaatgc gaagcaggaa tttttaagac gattgtttgc
    71281 agatgcggta actgaaatgg aaaattggga tgaatctgaa caaatccagt ttatcaaaaa
    71341 tgcattgtat tcattacctt tgacaggaaa agtagcattc attgctggag aaaaatcagc
    71401 agcctatctt tctcagacgt tgttggatga atggaacaat gaacttccct ttatgatggt
    71461 tttaagtgat gaaactgttg ctgatcaagc gggttttctg ataaacgatc aaggagttca
    71521 gtataatttt cttttcagca gccttgtaca agatatccaa ggaacgatga gttttgagat
    71581 tgccaatcaa ttattcgaat aaggagggat cagtgtatgg aataccacga actaaatccc
    71641 ttgattcgcg gaagagagct tgaacttttg tccaatgaga catttgaacg gatgattcaa
    71701 acaaattctg tcgaagcact tggtgaaata ttgaaatcca cgatttacag tccatatatt
    71761 tatgacggat atgaatatga cttcgaacag aatcttttaa aggagcagag ccgtctattt
    71821 cgctggttaa aggaatcagc accggagcct gaaatcgttt ggatctatac gatgcgttat
    71881 actttccata atctgaaggt tttgacaaaa gctgagatga caaatcaaaa tctggaccat
    71941 ttgtatatcg acgatggatt ttattcatta gaaaccttga aggatgccat tcatacgcaa
    72001 gtttcaaatg aacttcctga aagcattatg gacagtatac gagaagtacg tgattatttt
    72061 gaagagtcta ccattcttca gggaattgac gtgatttatg atcgacagtt tttgacagag
    72121 cagcgacggc ttgccgataa gctaggatat caagaattgc tgacagaagt cattgctttt
    72181 attgacttga ccaatatcac aacgatggct cgaggaatat tgcagaaaag aacatatggg
    72241 ttcatgacga cggtactttc cagctcggga agtatcgaaa aagaagtatt tttgaacttt
    72301 gtagataaag atatggaaga ttacacgaat tttctattag agacagatta tgctgatttt
    72361 ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa agcttgaaca gttgaaagat
    72421 gattatctaa gttcattgta tcaagaagca caaacccagg ctttcggacc actgccacta
    72481 ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc ttcgtttact ggttatcggc
    72541 aaaagaagcc atttcgaatc agaaacaatc agaaagcggg tgagacaagt ctatgacgca
    72601 taagatcggt gtagttggcg acaaagattc tgttttgcca ttcaaattgt ttggctttga
    72661 tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca attgaagaaa tggcaaagaa
    72721 tgagtatggc gtgatttatg tcacagagca atgtgcagca ttgagcccag aaacgatcga
    72781 acgttacaag gaacaaatga tacctgctat cgtattgatt cccaatcatc aaggatcatt
    72841 agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa gcagtaggtc aaaatatctt
    72901 atagtatgaa aaggagatag aaattttgca aattggaaaa atcataaaag tctccggacc
    72961 tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa gacatgtgtc tagtgggaga
    73021 tctaggagtt atcggagaaa ttatcgagat gcgtggcgat gtggcgtcta ttcaagtata
    73081 tgaagagact tctggaattg gtccaggcga accggttcgc tctactggtg aagctttatc
    73141 tgttgaatta ggaccaggga tcatttcaca aatgtttgac ggaatccaac ggccattaga
    73201 tacctttatg gaaatcacac aaagtaattt cttagggcgc ggagttcaat tgcccgcatt
    73261 ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa gccggagaaa cggtgagtgc
    73321 tggagatgtt atcgggatcg tagaagaaac aaaagttata aaacataaaa tcatggtgcc
    73381 aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt tcattcacta ttgatgaaac
    73441 agtatgtgtc attgaaaccg aaaatggtga caaaaatttg acgatgcttc aaaaatggcc
    73501 agtaagacgt agtcggccaa tcaaagaaaa attaaacccg gatgcaccca tgatcactgg
    73561 acagcgagtg attgatacat tcttcccagt gacaaaagga ggagcagcag ctgtacctgg
    73621 tccttttggt gcagggaaaa cagtcgttca gcatcaaatt gcaaaatggg ccgatgtgga
    73681 catggttgtc tatgtgggat gtggcgagcg tggaaatgag atgacggatg ttttaaatga
    73741 attccctgaa ttgattgacc ctaatacagg agaatcgttg atggaacgta cggtattgat
    73801 tgctaataca tcaaacatgc cagtagcagc tcgagaagct tctatctata cgggaatcac
    73861 aattgcggaa tatttccgtg acatgggtta tgacgttgct atcatggcag actccacttc
    73921 acgatgggca gaagcacttc gtgagatgag cggacgtttg gaagaaatgc ctggtgatga
    73981 aggttatcca gcttatcttg gttcacggct tgcggaatac tatgaacgat ctggaagagt
    74041 cattgcatta ggatcagaag gtcgcgaagg aagtataacc gcaatcagtg ctgtatcccc
    74101 gtctggaggg gatatttcgg aaccagtcac acaaaataca ttgcgggtcg taaaagtatt
    74161 ctggggtctt gattccagtt tagcccaaaa aagacatttt ccttcgatcg attggataca
    74221 aagctattct ctttattcaa cagaagtagg gaagtatatg gatcatatcc ttcaagctga
    74281 ctgggcatcc atggtcaccg agggcatgcg tattttgcag gaagaacagc agctgaatga
    74341 aatcgtgcgt ttggtaggga ttgattccct ttcagataat gaccgtctga cattagaagt
    74401 ggcaaaatcc attcgtgaag attatttgca gcaaaatgct tttgatgatg tcgatacatt
    74461 cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc ttgacctttg gtgaagaagg
    74521 acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc atggacggta ccgtatcgat
    74581 tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca gaattagaaa aactagatca
    74641 aattattggt gaaatcaagc aaacgatgca aacgatcgtt tcagaaggag gcatgagcaa
    74701 tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc attgatggcg gttgaaaaag
    74761 ttgctggtgt caaatatgaa gaattaatcg aagtccgtat gcaaaatggc gagattcgtc
    74821 gtggacaagt attggaagtc caagaagata aagcaatggt ccaaatattc gaaggaacaa
    74881 gtggtatcaa tttacgtgac tcttctgtac gatttttagg ccatccttta gaactaggtg
    74941 tatcggaaga catgatcggt cgagtattcg atggtttggg acgaccgaaa gataacggac
    75001 cagagatcct gcctgaaaag catttggata ttaacggaga agtaatcaat ccagtagctc
    75061 gggattatcc agatgagttt atccaaacag gtatctctgc aatcgatcat ttgaatacat
    75121 tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg tcttcctcat aaagaattag
    75181 ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc agatgatttc gccgtagtct
    75241 ttgctgctat tggaatcact tttgaagaag ctgaattttt catggaagat tttcgtcaaa
    75301 cgggcgcaat cgaccgttct gtcatgttta tgaatttagc aaacgaccct gcaatcgaac
    75361 gtatcgctac tcctcgtatg gctttgactg ctgcagaata tcttgcttat gaaaagggaa
    75421 tgcatgtttt ggttatcatg acagacatga ccaactatgc agaggcattg cgagagattt
    75481 ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc tgggtatctt tacacgaact
    75541 tagctacttt attcgaacga gcaggaagaa tccgcggact aaaaggatcg gttactcaaa
    75601 ttccgattct aacaatgcca gaagacgata aaactcatcc aatccctgac ttgactggtt
    75661 atattacaga aggacagatc atcttgacta gagagcttta caagagtggg attcagccac
    75721 caattgatgt actcccgtct ctttcgcggt taaaagataa gggaactgga cccggaaaga
    75781 caagagaaga ccatgcagct acgatgaatc agctttttgc agcctatgca caaggaaaac
    75841 aggcaaaaga attagccgta gttttaggtg aatctgcttt atctgatgta gacaagatct
    75901 atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa tcaaggattc tacacgaacc
    75961 gttcgattac tgaaacactt gatctagggt gggaactgct atcgatgttg ccaagaaccg
    76021 aattaaaacg aatcaaagac gacatgctgg acaaatatct gccagaaggg aagtgagttc
    76081 tgatggcacg attgaatgtg aatccgaccc gcatggaatt gactcgattg aaaaaacagt
    76141 tgacaactgc cacaagaggg cataaattat taaaagataa gcaagatgaa ctgatgcgtc
    76201 aattcatcct gttgattcga aagaacaacc agctgcgtca agaaatggaa gagtctatga
    76261 cagaagcaat gtctgatttt gtattggcaa atgcttcgat cacggaacct ttcatcgaag
    76321 aattatttgt tttgccggcg gaaaacgttg atttgtctat cgtagacaaa aatatcatga
    76381 gtgtaaaagt acctgtgatg aattttgaat atgatgaaca gctgacagac acacctttgg
    76441 aatacgggta tttgaattcc agtgccgaac tggatcggtc aatcgatcgg tttactaccc
    76501 tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac ttgtcaactg atggcgagtg
    76561 aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata tatgacgatc cctcaattag
    76621 aagaaacgat ctattatatt cgtatgaagc tggaagaaaa cgagcgcgca gaagtaacaa
    76681 ggttgatcaa agtaaaaaat atgggtacca ctgactaagt ggtatctttt tttaaaagag
    76741 gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt ttgccggctg ttcagcttct
    76801 cgcattagga ttttttctcc tgattttagt tggcggcggc ctacttaccc taccattttt
    76861 tagtaatagt gggcatggga caaattttat cgatgcgctt ttcactgcaa cttctgctgt
    76921 ctgtgtaacg ggattgacaa cattgaatac agcagagcat tggaacagtg ctggtcaatt
    76981 tttgatcatg cttttgattg agatcggcgg tcttggtttt atgatggttc cgattctatg
    77041 ctttgcattg ataaagaaga aagtcagtct cagcacacgt attatattaa aagaagcgct
    77101 gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt tacatcctaa aattggcctt
    77161 tgtgatccaa ttgattggta cggttgcttt atcttttgta tttgtgcctg aatttggctg
    77221 gttgaaaggt atctggtaca gtttgttcca tgcaatctct agtttttgta atgcagggtt
    77281 tgatttgtta ggagacagtt tagccaatca ccagcaaaat gtttatttga tcatggtggt
    77341 atctgctttg atcatttctg gtgggttagg cttcatcgta tggcgtgatt tactggaata
    77401 tcatcagaaa aagaagatga cgattcattc aaaaatcgca ttaaccgtta caggtaccct
    77461 tttattaggc ggattcgttg tgttcttttt cacagagcgc aatggggcaa cgctagtgga
    77521 agggaatatg tttcaacgat tggcaaacac cttttttatg agtgtgacac ctagaacggc
    77581 tggatattat tcagttgatt acctccatat gagccatgca ggtctgattt taacgatgtt
    77641 tttgatgtac atcggcggaa catctgggtc gacggccggc ggattgaaaa caactacgtt
    77701 aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga cgtacgagag cggaagcatt
    77761 tggccgtacc atccgacaag gcgcagtgtt gcgggcactt actttatttt ttgttacgtt
    77821 gacgttgtgt gtagtcgcca tcatgatttt atcggtgact gaaacgatac ctaaaacttc
    77881 tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt ggtacagtag gtcttacgat
    77941 gggattgacg ccagacttga ctgcaatagg gaaattgatc attatagcgc ttatgtacat
    78001 tggacgagtt ggtgtattga ctgtggtctt ctctctgatg acaaaagcac aaagaacaca
    78061 atcaaaatac aaatatccag aagaaagtgt catgattgga taatttcatc ttctgacaga
    78121 aacctagtga tttagtaaaa taagaaaata aaataatttt tcttgcttct tggatacttt
    78181 aagtgcaaaa tgttcgttta ttttttgagg cactacccgt tgttgaacct cgggtagtgc
    78241 ctctttttta ttgaaaggaa tcaataagat ttgattcctt tatttatcaa taggttataa
    78301 ttcatatttt tttatttttt cttttacgat acttagtcca gaagctaaaa tttcaaggtt
    78361 attagaggat agggaaattc gaacgaatgc atctggttgc gcacgtgtag taaagcgtct
    78421 ggaatgataa agtcggatac cgttagcaag aaagtcattc tcaattatct tagggtcaat
    78481 ctcaggatca actggaagaa ttcgatagta agggacacga ttcgttggtt tttctacttt
    78541 gaaaagagaa tcaaaaagct gattggctct ttttgtaaga cttaatttag tttctaagat
    78601 cctatttgct tcatttgaat ggaggatttg agtgatgact tccacatcta atccagaagt
    78661 tttcacattt atattaaaaa ttgcttgttc tatcttgctt tggacatgta ctggaactac
    78721 aagataagca actcgcaatc cagaacaaat aaattttgtc attccagcta agtagattgt
    78781 ttggttaggt aataaatttt ggaaaggagg aatgatcgta tgttgatagt aagttgttaa
    78841 aaatgaatgg atatcatctt caataaccca tagaccttct ttttcaatca cttcggctaa
    78901 ttgctctcgt cttttgtgag agatttgaaa ccctatagga ttattacaag aaggcataag
    78961 aaagacacca tgaatcttct tttttctaca ttcctgaatc aatatatcag gtcgcattcc
    79021 ttgctcgtca aaatcaattg gaacgatctc taaatgatat aactgagcca attcaatgaa
    79081 attagcgtat gtatatcggt ctgttgctat tctatcacct ggagagaaaa gtgcagaaag
    79141 tgtgatagcc aatccatttt gaacaccgga aacaatagat agagattcta aggacggaat
    79201 gactccttga gtttttaacc aatttgctgc agtagtcaat tgaaaatttg tgcctttggg
    79261 atcttggtaa ttaagcaaac caacaagatt tgggttatta ctagttgatt gaataaacgg
    79321 gacaacgaat ttattacatt cttcaaaaga actaaccaga ccaaaatcaa tgatatgttc
    79381 ggttacttca ttggtagaga tggtgatgga ctcaaagaca ttgggagaaa taaatgtgcc
    79441 acttccaata ttcgtataca gcaaaccttt ctcaattcca tatttataag ccttgccaac
    79501 ggtagtaaaa tttaaatcta aataatcagc tagctctctt tgggagggta actgagtgtt
    79561 tttctgtaat ttattcttta aaatatcttg ttctaattgt tgaatcaacg atcgataata
    79621 aggacgcttc aatttttgtt tgtctggtcg ccaagttaac gggtaattgt cataagaatt
    79681 aataggcatt ttctccactc cttaaaattg tataccatac aattttacca ttgattgtat
    79741 tgattttaga agttaatctt ttgtgaggag ggaattgtct atgaaaatta ttaattttga
    79801 ggaagtagta gcttctttta gttttgaaga agcgattcaa gtaatgaaaa aatgtttttt
    79861 tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg gtggaaattt tacctgacgg
    79921 aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg ggggagaaca gatattttgg
    79981 cacaaaagtc attaccgctt ttccggataa tcatcagtat catttacctt ctcatttagg
    80041 agagattttc ttgtttgatg ctcaaaacgg actacccaaa gcattgataa acgcaaatgc
    80101 agtcacctgg attcgaacag ctgctgtatc tgcgctggca acagattact tagcaaatcc
    80161 gcatgcagaa acacttgctt taattggagc tggtcaacaa gcattgtcgc atttgactgc
    80221 gatttctacc attcgtccca tcactacagt gtttgtttac gatattttag aagaaagacg
    80281 aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa ataacgttta taaatgcaat
    80341 atcagtagaa gatgcagtac gtccagcgga aatcatttgt acgttgacct ctagtaaaga
    80401 ggcatttctt caaaaagagt gggtaaggga agacgcacat atcaatgctg tgggcacatt
    80461 tactcctgat acaagagaga taagtacatc gttgatggaa acttctcaaa tctatgtaga
    80521 tgattactca gctgccttaa cagaaagcgg ggacctgctt atagccatgg cagaaggaga
    80581 tttagaggaa tcttgtattg ttggtacatt aggcgaatta gtttctgaga aaaaaacatt
    80641 ttataaagga gaatcgaggc cgacgatttt cgacgcagtt ggtctagcta ttgaagatct
    80701 ttgttgtgca gaatatattt ataacaaatt agagaaaggg gctttaaaat gaacttagat
    80761 tattacgtag ttgatgcatt tgcagatgag gtgttcaaag gaaatccagc agcggtttat
    80821 gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa ttgccattga aaacaattta
    80881 tctgaaacag catttacagt gaagaaaaac caagaatttg agcttagatg gtttacgcca
    80941 gatcgagaaa tcgatctttg tggtcacgcg acattagcaa cagcatttgt tttatttaac
    81001 tattacaaaa tacctgatga aacgattaaa ttttctactc aaagcggtaa tctatttgtg
    81061 acaagaatta aagattatta ttacatggat tttcctagta ttatgccgaa aaaagtgccg
    81121 atattgtctg aatatgaaga agcgattggg gctaagatta aagaagccta cttagcacgt
    81181 gatttatttt tcgtattaga agatgaaaaa acggtggcta aattgcagcc agattttaca
    81241 gctatcaaaa attttgaatt aggaatagga gtcatcgtga cagcagaaag tgtgcaaaaa
    81301 gactttgttt ctagaacctt ctttccaaaa ttaacaatca atgaagatcc agtatgtgga
    81361 tcagcacatt ctaatcttat tccttattgg gcagaaagaa tgaataaaac aaaattgtca
    81421 gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg aattaaaaga agatcgtgtg
    81481 ataataggag gaaaagcagt tttgttttct aaaggtacct cgtttatttg atttctttca
    81541 aatgaattaa aaataaattg gtggagagtg gcaaccttat agctacaatg gttgttaaaa
    81601 aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc tcattttaat tattggtgtt
    81661 tgttttttat tcaatcaaga gtaaaatgcc ccatactaac tatcctcgga tgacttcgat
    81721 tttataacca tcagggtcaa tgacaaagta gtaagaaggc gcagtgccag gtaatccttt
    81781 taagtcagta atctcaagac cagcagcttt atgttgctcg tgtaattttt ctagatcatc
    81841 agctgctata gcgatatgac catagccgtc tcctagatca tacgcaccat gatcatagtt
    81901 gtaagtcaac tctaattcat agtcatcccc aggaagtgtc aaataaacaa gtgtaaattt
    81961 gttttctgga aaatcacggc ggcggctttc ttcaaaacca aatgcctttt gataaaattc
    82021 gattgatgct tcaagatctt tcactcgaac acatgtgtga gccattttca tagattcatc
    82081 tctccttttc cttttactca atcataacaa aaagaagtaa ggaaatagtt gatttgtgct
    82141 tgttcatgag aaatttctca tgtaaaatgg atagaaaata aaactgttaa ggtggaaact
    82201 attcatggat aaaccaattt ttggtgaaaa aaagcaattt gctgattatc aaacccgtta
    82261 tgcagcatat attgtcatag aaagagatca gcagattgca gtaatcgagg ctccaaatgg
    82321 tgcttttttt cttcctggcg gggaaattga aggagaagag acgaaagaag aagcgattgt
    82381 ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt tcttcttatc tgggagaagc
    82441 tgatgagtat ttttactcga attatcgtgc aacttattat tataatccag gatatttctt
    82501 tgttgctgat tcttggaagc gtataggaga accaactgaa aagaccaata aaatttggtg
    82561 ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt agccaccgat gggcagtcga
    82621 gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt agatcagctt tctctcagtt
    82681 tttttattta ttacagtaca tattttttga ttgcttcagc tacaccgtgc tcgtcgtttg
    82741 atgaagtgat gacatttgct gcttctttga caagagaaac ggcatttccc attgctactc
    82801 ctagaccagc atattcaatc atggataagt cgttctcgtt atctccgata gccatgattt
    82861 catcttgatc aataccaagg tgctgtgcga gattcgcaac tgccgcacct ttacttgctt
    82921 ctttatttag tacttcgaag taatagggcg cacttttgac cgttgtgtat ttatcacgga
    82981 aagtttgcgg taaacgcgca attgctgcat ctaaaatttc tggttcgtcg atcatcatca
    83041 ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt tcgatatttt agcggcatct
    83101 tgactaaact tgcttcgtgg atcgtataag cgctgatatc tcgattaggc gtgtagatcg
    83161 ctttgtcatc gatcgaatgg agatgactgc cgacacggcg tgccattact tcgatctcta
    83221 ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt acctgatttc gtttgttgta
    83281 cgagtgcccc gttataagta ataacgtaat cattatcttg aaaaaggttc aactcgttta
    83341 actgttcctg gacacctggt aagggacggc cagtgcataa aacgatcgaa acaccttttg
    83401 caacagcagc ggcaatcgct tcttttactt cttttgttac ttctcttttc tcatttaata
    83461 aagtaccatc gatgtcgata gcgactagtt tgatagacat aatttcctcc aatatatttt
    83521 aatttatttc aatcaatgcg ccattacgga tatggctggc aaattcttga taagtttcgt
    83581 caaataaatc ataatgatca cgcaaggacg gatcaaccat ttcttttgga aagaaaaatc
    83641 gctcatctcc ttgttcttgt cccgctaatg cagcaacaag ctgactaacc tgtgataatt
    83701 ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt gcgatgtctg ccttgttctg
    83761 ggcgataaaa atcataaggt agatcgtagc tagaattcac agcagtgtaa taaacaggat
    83821 tatatccgac cttttctact aacaaagtga gttcttggac taattttgaa tcccggttgc
    83881 cagaaaaagt agcagatttc agtggtttac gattcaaaaa acgtttagct aaatcattta
    83941 aaataggatc agcagattca gaccattgag taaagtaagt gcccaagacc ccatcatcaa
    84001 gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa tggcagaagt aattgagaat
    84061 ggagttcaaa actgctggca tcttcttgat acaactcgtg tgcgcgatga agcagatgct
    84121 ccaaaattac ttccatacca cgggaaacag gatggaagta cacttgcaca tacatttgat
    84181 atcggctaac gatataatct tccactgcat gcatgccact catggaaaaa gcgatcccgc
    84241 cttcataagg acgaatcaca cgtaatattc gggtaagatc aaatgttcca tattctgttc
    84301 cagtgaaata agcatcccga agcaaatagt ccattcgatc tgcatcgatc tgactagaaa
    84361 tcatttggac aacttgtggg ttggggtaag ttttttggat cacactagct accttttcag
    84421 gaaaaccttc ttctactcgg ttaagaatct gatacacttc tgtttctgga gaagtgatga
    84481 tctctacagt gatggcttca tgattcgtat ggaagatatg ttcaaatgta tgagagtaag
    84541 gaccatgacc gacatcatgc aacagtgccg cacataaagt caccagccgt tcctgatcat
    84601 cccagcctcc gttgcctatt ttttcttttg aaaaatttcg agaaaagata tcgcagattc
    84661 gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg gctatgttct gctccgtgaa
    84721 aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag acgttggact tctttcgaat
    84781 tgatcaaatc taagattact tgatgctgga catggatata gttatggact ggatcacgaa
    84841 aaactttttc cataggcagc atttgatatt tataaggggt tgtcataatg tagctccttt
    84901 atgttattac gttgttccat tctctggatt tggttgttcc attgctccag ctggatttct
    84961 ttttttagcc agtcggtaga atcctgtaaa tcacttgcag aatacagctt ttcaaaagct
    85021 tttgcaaatg cttctttgac ttcttttaca aaaaggggtt ggtcaagcag ttcttcaagt
    85081 gttgccattg tagcaggatc cactgcagga tagccatttg taccaaagtc ttcttttaaa
    85141 gcagattgat aaaatcgctt catgacttgc ccacgttttt cctgattacc gttgacactt
    85201 aagtacatca taacagaaat cccatttttg atccgacgct gagcgatacc ggcaaatttt
    85261 ttgccatgga tgcttaaatc ataagttcca ggacaataag aatgggtgat ttcgaatgct
    85321 tgtatcggtg cctttggaaa agcgtattta gtcaatagga gcatttgctc gtaagcgtca
    85381 tctatggtta ttttttttgt gtcactttgg gggaaaatca aagaaatatt caaaattcct
    85441 tcatctgata cgatccctaa tccacctgaa tttctgatca ctggagcgta acctgcatct
    85501 tccagtgttt ggatcccgtc acttagaaaa ggagttcgaa catccttcat acctaagatg
    85561 actgtgcagt catattgcca gaaatgtaaa aagatttgct ggtgttcttt agaatattcc
    85621 gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt tttgataaat gtgctgatct
    85681 agtcgaatcg cagggttcat tcaaccggct cctttgtata aattcatatt ttctacaata
    85741 ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt ttctgtaaaa atggacaagt
    85801 tggttgacaa aacaaaagaa actaggaaca atgtagctat gagaagtaaa ggagaatgaa
    85861 taatggaatt atcacaagga acaccgattt caatcaaatt gcgtacaaaa gtaaaacagc
    85921 aaggggaagt gcaagatttt tattttgatt tgaaagggca aatggtgaca attggcgata
    85981 cgctgtacat acgttataaa gagattcagg aggagacggg cgatgagatt cctgtaacga
    86041 tcaaattagt tccagatggt catgtacagc tgatccgtgc aggagaaatg cggatgcgtt
    86101 tgaaatttgg ctataaagaa cggttagaaa catcctatcg aacaccatac ggtatgatcc
    86161 agattgctac ttttacgaaa gaactgcatg ttagtttgaa agaccgtccc acagcaggga
    86221 aggttcgaat cgattatgat ctgttcatgg gaccggaaaa aatcggcgaa tattatttga
    86281 ccttggattt tacagcgtaa atataaaaga aaagtttgat tttttgtgtc tgattttgta
    86341 tgataaggag tagactgtga aaggacgtgt cctgattgga aattagtgta tttgaaggtg
    86401 caaacaaaag cgaattatca atgatcgaag tagcacacgc gatcttagaa caacgtggcg
    86461 atgtcatgga tttttctgat ttagctaacc agatccaaaa ctatcttggg aaatcagaca
    86521 gtgaaattcg tgattcactc gcacaatttt atactgactt gaatattgac ggcagcttta
    86581 tctcattagg ggataatcgt tggggcttgc gttcatggta tgctatcgat tcgatcgatg
    86641 aagaagtaaa ccatggaatg gacgaagagg atgaagatac tccacgtcgc cgtaaacgca
    86701 agaaagtcaa cgcctttatc aatgatgatg aagacgcgat cgattacaat gacgatgacc
    86761 cagaagatac agatctgaca gaagaagatg acgatgatct gtttgatgac gatgatgatg
    86821 aagatgaaga aatcgctgct tataattctg acttgcaaga aatcggagca gatgacccga
    86881 cagatgatga agaagacttg ccaggaaata tcgaagaaga tttaagtatc atagacgaag
    86941 acgatgatga tgaagattat gaagaggaag aattttctga ctctgaaaaa gaataaacat
    87001 tattttctta cgagaggttg tgacagagac gatcagccta tcagaataag gaggtgtgac
    87061 acgatttttg t
//
LOCUS       DAZAJZ010000002        84929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000002 DAZAJZ010000000
VERSION     DAZAJZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.002"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..516
                     /locus_tag="ACG86D_000090"
     CDS             <1..516
                     /locus_tag="ACG86D_000090"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3139274.1"
                     /translation="PDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEMS
                     PYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKT
                     YIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVEL
                     KKKRRRTQKFL"
     gene            644..1327
                     /locus_tag="ACG86D_000091"
     CDS             644..1327
                     /locus_tag="ACG86D_000091"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN3139275.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1636..1709
                     /locus_tag="ACG86D_000092"
     tRNA            1636..1709
                     /locus_tag="ACG86D_000092"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1670..1672,aa:Arg,seq:tct)
     gene            1868..2227
                     /locus_tag="ACG86D_000093"
     CDS             1868..2227
                     /locus_tag="ACG86D_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN3139276.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2350..3981
                     /locus_tag="ACG86D_000094"
     CDS             2350..3981
                     /locus_tag="ACG86D_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN3139277.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4043..4684)
                     /locus_tag="ACG86D_000095"
     CDS             complement(4043..4684)
                     /locus_tag="ACG86D_000095"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN3139278.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4870..4946
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4966..5274
                     /gene="rplU"
                     /locus_tag="ACG86D_000096"
     CDS             4966..5274
                     /gene="rplU"
                     /locus_tag="ACG86D_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN3139279.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5301..5645
                     /locus_tag="ACG86D_000097"
     CDS             5301..5645
                     /locus_tag="ACG86D_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN3139280.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5658..5951
                     /gene="rpmA"
                     /locus_tag="ACG86D_000098"
     CDS             5658..5951
                     /gene="rpmA"
                     /locus_tag="ACG86D_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN3139281.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6385..9192
                     /locus_tag="ACG86D_000099"
     CDS             6385..9192
                     /locus_tag="ACG86D_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3139282.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9383..9874
                     /locus_tag="ACG86D_000100"
     CDS             9383..9874
                     /locus_tag="ACG86D_000100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN3139283.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9977..10966
                     /locus_tag="ACG86D_000101"
     CDS             9977..10966
                     /locus_tag="ACG86D_000101"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN3139284.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10999..11802
                     /locus_tag="ACG86D_000102"
     CDS             10999..11802
                     /locus_tag="ACG86D_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN3139285.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11823..12737
                     /locus_tag="ACG86D_000103"
     CDS             11823..12737
                     /locus_tag="ACG86D_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3139286.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12820..13194
                     /locus_tag="ACG86D_000104"
     CDS             12820..13194
                     /locus_tag="ACG86D_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN3139287.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13333..13578
                     /locus_tag="ACG86D_000105"
     CDS             13333..13578
                     /locus_tag="ACG86D_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3139288.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13750..14814
                     /locus_tag="ACG86D_000106"
     CDS             13750..14814
                     /locus_tag="ACG86D_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN3139289.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15060..15485
                     /locus_tag="ACG86D_000107"
     CDS             15060..15485
                     /locus_tag="ACG86D_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3139290.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15478..15927
                     /gene="nusB"
                     /locus_tag="ACG86D_000108"
     CDS             15478..15927
                     /gene="nusB"
                     /locus_tag="ACG86D_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN3139291.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16024..16878
                     /locus_tag="ACG86D_000109"
     CDS             16024..16878
                     /locus_tag="ACG86D_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN3139292.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16879..18216
                     /gene="xseA"
                     /locus_tag="ACG86D_000110"
     CDS             16879..18216
                     /gene="xseA"
                     /locus_tag="ACG86D_000110"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN3139293.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18221..18445
                     /locus_tag="ACG86D_000111"
     CDS             18221..18445
                     /locus_tag="ACG86D_000111"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN3139294.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18447..19337
                     /locus_tag="ACG86D_000112"
     CDS             18447..19337
                     /locus_tag="ACG86D_000112"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN3139295.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19346..20164
                     /locus_tag="ACG86D_000113"
     CDS             19346..20164
                     /locus_tag="ACG86D_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN3139296.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20271..20720
                     /locus_tag="ACG86D_000114"
     CDS             20271..20720
                     /locus_tag="ACG86D_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3139297.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20738..22420
                     /gene="recN"
                     /locus_tag="ACG86D_000115"
     CDS             20738..22420
                     /gene="recN"
                     /locus_tag="ACG86D_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN3139298.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22475..22591)
                     /locus_tag="ACG86D_000116"
     CDS             complement(22475..22591)
                     /locus_tag="ACG86D_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN3139299.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22664..23620)
                     /locus_tag="ACG86D_000117"
     CDS             complement(22664..23620)
                     /locus_tag="ACG86D_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3139300.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23791..24159
                     /locus_tag="ACG86D_000118"
     CDS             23791..24159
                     /locus_tag="ACG86D_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN3139301.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24329..24760
                     /gene="mraZ"
                     /locus_tag="ACG86D_000119"
     CDS             24329..24760
                     /gene="mraZ"
                     /locus_tag="ACG86D_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN3139302.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24781..25740
                     /gene="rsmH"
                     /locus_tag="ACG86D_000120"
     CDS             24781..25740
                     /gene="rsmH"
                     /locus_tag="ACG86D_000120"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN3139303.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25745..26146
                     /gene="ftsL"
                     /locus_tag="ACG86D_000121"
     CDS             25745..26146
                     /gene="ftsL"
                     /locus_tag="ACG86D_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN3139304.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26147..28339
                     /locus_tag="ACG86D_000122"
     CDS             26147..28339
                     /locus_tag="ACG86D_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN3139305.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28365..29327
                     /gene="mraY"
                     /locus_tag="ACG86D_000123"
     CDS             28365..29327
                     /gene="mraY"
                     /locus_tag="ACG86D_000123"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN3139306.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29341..30702
                     /gene="murD"
                     /locus_tag="ACG86D_000124"
     CDS             29341..30702
                     /gene="murD"
                     /locus_tag="ACG86D_000124"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN3139307.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30728..31816
                     /gene="murG"
                     /locus_tag="ACG86D_000125"
     CDS             30728..31816
                     /gene="murG"
                     /locus_tag="ACG86D_000125"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN3139308.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31831..33051
                     /locus_tag="ACG86D_000126"
     CDS             31831..33051
                     /locus_tag="ACG86D_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN3139309.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33207..34532
                     /gene="ftsA"
                     /locus_tag="ACG86D_000127"
     CDS             33207..34532
                     /gene="ftsA"
                     /locus_tag="ACG86D_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN3139310.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34555..35796
                     /gene="ftsZ"
                     /locus_tag="ACG86D_000128"
     CDS             34555..35796
                     /gene="ftsZ"
                     /locus_tag="ACG86D_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN3139311.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35813..36490
                     /locus_tag="ACG86D_000129"
     CDS             35813..36490
                     /locus_tag="ACG86D_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN3139312.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36504..37103
                     /locus_tag="ACG86D_000130"
     CDS             36504..37103
                     /locus_tag="ACG86D_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN3139313.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37184..37405
                     /locus_tag="ACG86D_000131"
     CDS             37184..37405
                     /locus_tag="ACG86D_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN3139314.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37454..38236
                     /locus_tag="ACG86D_000132"
     CDS             37454..38236
                     /locus_tag="ACG86D_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN3139315.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38257..38964
                     /locus_tag="ACG86D_000133"
     CDS             38257..38964
                     /locus_tag="ACG86D_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN3139316.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39226..42024
                     /gene="ileS"
                     /locus_tag="ACG86D_000134"
     CDS             39226..42024
                     /gene="ileS"
                     /locus_tag="ACG86D_000134"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN3139317.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42073..43599)
                     /gene="zwf"
                     /locus_tag="ACG86D_000135"
     CDS             complement(42073..43599)
                     /gene="zwf"
                     /locus_tag="ACG86D_000135"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN3139318.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43614..44261)
                     /locus_tag="ACG86D_000136"
     CDS             complement(43614..44261)
                     /locus_tag="ACG86D_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN3139319.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44445..44774)
                     /locus_tag="ACG86D_000137"
     CDS             complement(44445..44774)
                     /locus_tag="ACG86D_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN3139320.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44951..45679
                     /gene="sfsA"
                     /locus_tag="ACG86D_000138"
     CDS             44951..45679
                     /gene="sfsA"
                     /locus_tag="ACG86D_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN3139321.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45695..46708
                     /locus_tag="ACG86D_000139"
     CDS             45695..46708
                     /locus_tag="ACG86D_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3139322.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46708..47985
                     /locus_tag="ACG86D_000140"
     CDS             46708..47985
                     /locus_tag="ACG86D_000140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3139323.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48048..50750
                     /locus_tag="ACG86D_000141"
     CDS             48048..50750
                     /locus_tag="ACG86D_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3139324.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50902..51219
                     /locus_tag="ACG86D_000142"
     CDS             50902..51219
                     /locus_tag="ACG86D_000142"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3139325.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51249..51569
                     /locus_tag="ACG86D_000143"
     CDS             51249..51569
                     /locus_tag="ACG86D_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3139326.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51677..53137
                     /locus_tag="ACG86D_000144"
     CDS             51677..53137
                     /locus_tag="ACG86D_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3139327.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53205..53426
                     /locus_tag="ACG86D_000145"
     CDS             53205..53426
                     /locus_tag="ACG86D_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139328.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53457..53639
                     /locus_tag="ACG86D_000146"
     CDS             53457..53639
                     /locus_tag="ACG86D_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFN3139329.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53639..54052
                     /locus_tag="ACG86D_000147"
     CDS             53639..54052
                     /locus_tag="ACG86D_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN3139330.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54175..55356
                     /locus_tag="ACG86D_000148"
     CDS             54175..55356
                     /locus_tag="ACG86D_000148"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139331.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55427..55591)
                     /locus_tag="ACG86D_000149"
     CDS             complement(55427..55591)
                     /locus_tag="ACG86D_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139332.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55745..55818
                     /locus_tag="ACG86D_000150"
     tRNA            55745..55818
                     /locus_tag="ACG86D_000150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55779..55781,aa:Arg,seq:cct)
     gene            complement(55887..57026)
                     /locus_tag="ACG86D_000151"
     CDS             complement(55887..57026)
                     /locus_tag="ACG86D_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3139333.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57198..57332
                     /locus_tag="ACG86D_000152"
     CDS             57198..57332
                     /locus_tag="ACG86D_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139334.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57325..57960)
                     /locus_tag="ACG86D_000153"
     CDS             complement(57325..57960)
                     /locus_tag="ACG86D_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139335.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58073..58708)
                     /locus_tag="ACG86D_000154"
     CDS             complement(58073..58708)
                     /locus_tag="ACG86D_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFN3139336.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58742..59203)
                     /locus_tag="ACG86D_000155"
     CDS             complement(58742..59203)
                     /locus_tag="ACG86D_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFN3139337.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59333..59764)
                     /locus_tag="ACG86D_000156"
     CDS             complement(59333..59764)
                     /locus_tag="ACG86D_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN3139338.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59782..60102)
                     /locus_tag="ACG86D_000157"
     CDS             complement(59782..60102)
                     /locus_tag="ACG86D_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3139339.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60401..61177
                     /locus_tag="ACG86D_000158"
     CDS             60401..61177
                     /locus_tag="ACG86D_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN3139340.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61192..61395
                     /locus_tag="ACG86D_000159"
     CDS             61192..61395
                     /locus_tag="ACG86D_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139341.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61411..61749
                     /locus_tag="ACG86D_000160"
     CDS             61411..61749
                     /locus_tag="ACG86D_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFN3139342.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61736..61915
                     /locus_tag="ACG86D_000161"
     CDS             61736..61915
                     /locus_tag="ACG86D_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139343.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(61958..62428)
                     /locus_tag="ACG86D_000162"
     CDS             complement(61958..62428)
                     /locus_tag="ACG86D_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139344.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62515..63213
                     /locus_tag="ACG86D_000163"
     CDS             62515..63213
                     /locus_tag="ACG86D_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN3139345.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63391..63732
                     /locus_tag="ACG86D_000164"
     CDS             63391..63732
                     /locus_tag="ACG86D_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139346.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63725..64396
                     /locus_tag="ACG86D_000165"
     CDS             63725..64396
                     /locus_tag="ACG86D_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN3139347.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64402..65094
                     /locus_tag="ACG86D_000166"
     CDS             64402..65094
                     /locus_tag="ACG86D_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN3139348.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65091..65840
                     /locus_tag="ACG86D_000167"
     CDS             65091..65840
                     /locus_tag="ACG86D_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139349.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65852..66121
                     /locus_tag="ACG86D_000168"
     CDS             65852..66121
                     /locus_tag="ACG86D_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139350.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66127..66291
                     /locus_tag="ACG86D_000169"
     CDS             66127..66291
                     /locus_tag="ACG86D_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139351.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66284..66583
                     /locus_tag="ACG86D_000170"
     CDS             66284..66583
                     /locus_tag="ACG86D_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139352.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66583..66885
                     /locus_tag="ACG86D_000171"
     CDS             66583..66885
                     /locus_tag="ACG86D_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN3139353.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            66925..67290
                     /locus_tag="ACG86D_000172"
     CDS             66925..67290
                     /locus_tag="ACG86D_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139354.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67780..67920
                     /locus_tag="ACG86D_000173"
     CDS             67780..67920
                     /locus_tag="ACG86D_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139355.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            67917..68099
                     /locus_tag="ACG86D_000174"
     CDS             67917..68099
                     /locus_tag="ACG86D_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139356.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68112..68306
                     /locus_tag="ACG86D_000175"
     CDS             68112..68306
                     /locus_tag="ACG86D_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139357.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(68339..68551)
                     /locus_tag="ACG86D_000176"
     CDS             complement(68339..68551)
                     /locus_tag="ACG86D_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139358.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            68679..68879
                     /locus_tag="ACG86D_000177"
     CDS             68679..68879
                     /locus_tag="ACG86D_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139359.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            68876..69148
                     /locus_tag="ACG86D_000178"
     CDS             68876..69148
                     /locus_tag="ACG86D_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFN3139360.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            69149..69334
                     /locus_tag="ACG86D_000179"
     CDS             69149..69334
                     /locus_tag="ACG86D_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139361.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            69312..69668
                     /locus_tag="ACG86D_000180"
     CDS             69312..69668
                     /locus_tag="ACG86D_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139362.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            69628..70104
                     /locus_tag="ACG86D_000181"
     CDS             69628..70104
                     /locus_tag="ACG86D_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN3139363.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(70235..70963)
                     /locus_tag="ACG86D_000182"
     CDS             complement(70235..70963)
                     /locus_tag="ACG86D_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFN3139364.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            71011..71289
                     /locus_tag="ACG86D_000183"
     CDS             71011..71289
                     /locus_tag="ACG86D_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139365.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            71291..71677
                     /locus_tag="ACG86D_000184"
     CDS             71291..71677
                     /locus_tag="ACG86D_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFN3139366.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            71674..72054
                     /locus_tag="ACG86D_000185"
     CDS             71674..72054
                     /locus_tag="ACG86D_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN3139367.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            72166..72618
                     /locus_tag="ACG86D_000186"
     CDS             72166..72618
                     /locus_tag="ACG86D_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN3139368.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            72615..74342
                     /locus_tag="ACG86D_000187"
     CDS             72615..74342
                     /locus_tag="ACG86D_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3139369.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            74406..75593
                     /locus_tag="ACG86D_000188"
     CDS             74406..75593
                     /locus_tag="ACG86D_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3139370.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            75565..76134
                     /locus_tag="ACG86D_000189"
     CDS             75565..76134
                     /locus_tag="ACG86D_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFN3139371.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            76147..77511
                     /locus_tag="ACG86D_000190"
     CDS             76147..77511
                     /locus_tag="ACG86D_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3139372.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            77513..77791
                     /locus_tag="ACG86D_000191"
     CDS             77513..77791
                     /locus_tag="ACG86D_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3139373.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            77772..78098
                     /locus_tag="ACG86D_000192"
     CDS             77772..78098
                     /locus_tag="ACG86D_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3139374.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            78088..78426
                     /locus_tag="ACG86D_000193"
     CDS             78088..78426
                     /locus_tag="ACG86D_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139375.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            78416..78781
                     /locus_tag="ACG86D_000194"
     CDS             78416..78781
                     /locus_tag="ACG86D_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN3139376.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            78788..79414
                     /locus_tag="ACG86D_000195"
     CDS             78788..79414
                     /locus_tag="ACG86D_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3139377.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            79414..79878
                     /locus_tag="ACG86D_000196"
     CDS             79414..79878
                     /locus_tag="ACG86D_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139378.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            80073..82382
                     /locus_tag="ACG86D_000197"
     CDS             80073..82382
                     /locus_tag="ACG86D_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN3139379.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            82394..83098
                     /locus_tag="ACG86D_000198"
     CDS             82394..83098
                     /locus_tag="ACG86D_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3139380.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            83095..>84929
                     /locus_tag="ACG86D_000199"
     CDS             83095..>84929
                     /locus_tag="ACG86D_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN3139381.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        28610 a        13990 c        17847 g        24482 t
ORIGIN      
        1 cctgattaca aaaaaataac ttttataagt tctataacgt tgattatatt tttaattctt
       61 attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat tattgaggac
      121 aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc gaaaaataca
      181 aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa aaaaatctcg
      241 acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa agctgataca
      301 aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag tcccatcttt
      361 cagattccat atattggact agtcctttca aaaacggcat ttgcatggat tcaggggtta
      421 ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt tgtagagttg
      481 aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca taaatgtatt
      541 ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg cagcttgtag
      601 atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca atgaaatgat
      661 gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc aaaaaaaatt
      721 accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac cagttattcc
      781 ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg aacgaatctt
      841 ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa tcggagaaaa
      901 cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta aaaaaaacta
      961 tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag cagatatctt
     1021 gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat caaaatatat
     1081 tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga tggtagatga
     1141 catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg gcaaacgagc
     1201 tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg atttgacatc
     1261 gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag aaacaatcga
     1321 gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg ctcaattcga
     1381 acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa atctaaataa
     1441 acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt agaacagctg
     1501 aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata attcattatt
     1561 tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat aattattgat
     1621 gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt ctaagcggac
     1681 ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg aggtttctaa
     1741 cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat aaaataaaaa
     1801 aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca gaaaagagga
     1861 agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg aattttttta
     1921 tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg tttttaaaat
     1981 gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc aaggagtgta
     2041 caatggacta ttaggagtgg gactacttta tggagcttat ctttcttccg catctaaaga
     2101 aatcacaagt atgctattaa tatctatttt ttttgttgca ttatatggca gtttgactag
     2161 cgatcgttgg attttactca aacaaggtgg gctagctctg ttggcattgc tttctatttt
     2221 cttataaata tagaaaaaat aagaatttat ctaacaggaa actttgtgaa aaatattact
     2281 ttatctcatg aaatatgata ttctaatgaa tggtatttta gaaaatttca tgaaaaagga
     2341 gttagatagg tgttcaaaaa ttatgtttcc tcactaaaaa aagaattttc aggttatgat
     2401 ctttcaagat ttcagaaaga tcttttggct gggatcacag tagctgctgt agcattgcct
     2461 ttagccttgg cttttggggt atcaagcgga gctgatgcgg ccgcaggttt gattacggct
     2521 atcattgccg gtatcgttat tggcggttta tcaggcggtt tttatcaaat atcaggtccg
     2581 acaggtgcga tggcagctat cttgatgtct attgcggcaa caaagggaat gcaaggtgta
     2641 ttgcttgcta cttttttagc gggtctattt ttacttgcag caggactttt ccgattagga
     2701 acactgactt cttttatccc agcacccgta attacggggt ttacttcagg tattgctatc
     2761 attattgcat taggtcagat cgataattta tttggtgttc actcggaagg tacaaacgtt
     2821 attgaaaaac taagtagtta tcaaacgata ggatttccga taaacacagc ctctttgatc
     2881 atgggagggc tagtcatttt agggatgttt atctatccaa aaaaatgggc gaaacgaatg
     2941 cctagttcgc tgctggctat cattttggca acagcagtta tgctattgtt ccatttacca
     3001 gtagcaactg tgggtaagat tccacaaaca ttgatcagta gtaatcgttt gaatatggga
     3061 gattttagct tttctgcctt gcaacaagtg gcagttccag ctattagcat tgctttatta
     3121 ggaatgattg aaagcttgct atgtggtgct tcggctggcc gaatggcaaa taagccctta
     3181 gatagcaatc aagaattaat ggcgcaagga attggaaatc tgttgcttcc tttctttggc
     3241 gggattcctg ctactgcggc catcgcccgt acaagcgtgg ccatcaaatc tggtgcacaa
     3301 actagattag ctggtatgat acatgcagtt gtcttactgc tctccatgct tgtttttgcg
     3361 ccagtgatgt cacagtttcc aatgccagca cttgcaggtg ttttgattgt gacggcttgg
     3421 cgtatgaatg aatgggaaac gatcaaggag ctatttacta ataaatattg gtcagcagtt
     3481 ctattgttta tccttacgat gatttgtaca gttatctttg atttaagtat tgctatcgtg
     3541 atcggggtga tcggcggctg tgtatttttt gttgtcaaaa gcgctgctat cacgatttca
     3601 ttagaaacga tcgattggca aagaatgggg ctgccagaaa cggaaaagct gaataactgg
     3661 acagtagtgt atattagtgg tccactgttt tttatgtctt cagaaaaact caaaaataaa
     3721 ttggaagaac taaatcatca cgatggtgtc attttctcca tgcgaggcgt accaagtatt
     3781 gatttgacgg cacgtgcagt ttttgatgaa tttcaagaaa aagcagagca aagaggacaa
     3841 acgattatct atacttcctt gcagccagaa gtagaaaaac aactttctca cttgtgggaa
     3901 catacattca cgcatcaaca tcaaacagtt gctcatgcgt tgacagcttt acatgcgaaa
     3961 tatactgcaa ttagccatta aaacaatgaa aaagaacaga caagactgta aactgaagat
     4021 tttatggtct tgtctgttct ttttaatatt gctgtaaaag tgaagctagt acttgccagt
     4081 cttgatggat aacaggtaca agggagcgat tgtagaaaat agctgctgga tgaaaaagtg
     4141 gaagaattcg atagtttttt tctgaccatt cgtaggcatc cttttgttct gttaattgta
     4201 agacaggccc tgtatagagt tgaccatgat ttttggtaac cgtatattct ttgccaagta
     4261 aacgttgaag accaatgttt ccaagtgtcg caattaattt tggctggacc ttcatcagtt
     4321 cgtaatccaa tattggtgca tgcgcaaata cttcggaacg agtaggtgtc cgatttggat
     4381 aaacagtttc tgactgttgt gtccgtttat tgatccgatg agtcacgcga tacgggcggc
     4441 tccttacagc actggtgata tacacctctt ctcgtgttaa gccagttgaa gaaagtgcct
     4501 gcatcaattc ttttccagct tgaccgctaa aaggaacatg cgtatcaatc tctgttttcc
     4561 ctggggcttc tcccacgagc atcagtttag gatgaagagg tccttgccca gcgataaatc
     4621 cttctacagg acggccgttc attcttaaag aagcttcttg tatcagttct tttgggtatt
     4681 ccatttatgc tcctcctttt tcttttatta taagaataaa agaaacacaa tgcgactatt
     4741 ttatttggta gaactgtgag gatagaaaag cagaagtatt gcttgaattt ttgttttact
     4801 ttttgatttt aaatttagtt gactaacggt tgctgttctg gtaagctagt actgttgtaa
     4861 ttgtggactc cacagctaca accgctcaga acaagtaata agcacagcat atgctgtcac
     4921 ttcggatggc gagtctaagt atatcagaag gaggtgcttt atagcatgta cgcaattatt
     4981 aaaactggtg gtaaacaagt aaaagttgaa gtgggacaag caatctatgt tgaaaaactt
     5041 gacgttgaag caggagaaaa agttgttttt gacgaagtta tcttagtagg cggagaatct
     5101 acaaaagtag gcgctccaac tgttgcaggt gcaactgttg aaggtactgt tgaaaaacac
     5161 ggcaaacaaa agaaagtcgt tactttcaaa tacaaaccta aaaaacattc acaccgtaaa
     5221 caaggtcatc gtcaaccata tacaaaagtg gttatcgacg cgatcaacgc ttaatagcgt
     5281 ttgagaaaga aggcctatat atgatcaaag gaacatttaa gcgaaatgac gcaggtcaga
     5341 tcgtttcatt cactttgacc gggcatgctg atgctggtcc ttatggaagt gatatcgttt
     5401 gtgcgggtgt ttctgcgtta gcgatcagca ctgtcaacgg gattgcctca ttagccggtt
     5461 ttgagccgat tgttgaaatg aatgaagaag aaggcggtta tctttatacc gaagtcactt
     5521 caggaatgac acaagaacaa aacaatattg ctgagattct attagaaaat cttttattag
     5581 gattacaatc gatagaagct gaaaactcag aatatatcca aattaaaaca attaatgata
     5641 aataggaggt gcagaccatg ttgttatcta tgaatctaca attatttgcc cacaaaaaag
     5701 gtggcggttc tacatctaac ggccgtgact cagaatcaaa acgtctaggt gctaaacgtg
     5761 ctgatggaca aacagttaca ggtggatcaa ttctttaccg tcaacgcggt acaaaaatct
     5821 atccaggtgt aaacgtgggt atcggtggag acgacacttt atttgcaaaa gttgatggcg
     5881 tagtacgttt cgaacgtaaa ggccgtaaca aaaaacaagt gtctgtttat ccagtagctc
     5941 aagaagctta attgaatctt atgattaaag aggttgtgac aagacttttg tcacagcctt
     6001 ttttgtttat aaaaaaaaaa gttgtagtgg aggctacgaa gtttactttg tctttttaca
     6061 cttaatattt ttttgccaat ttggaatcag gatgttttga taactatcgt tttcaattga
     6121 tggaagctat attgtttgct atcagtgaaa taattatatg gtaaacagtg ttctttttct
     6181 tgtttccatt ataaaaatcg tatcgggatt tttcgttctt ttaaattgaa catttctatc
     6241 tatccgcgtg ttatcaacaa aacaaaatca ggtaaatagt tgacgaaaca gaaataaata
     6301 catagtatta tgttttgaag cctaagtaca atagtctata gaaaaggagc gataccattg
     6361 gcaaaacgca ttgaccgaat ttatatgtat gtcaaagaaa aaaccgctca tttaacaaag
     6421 gcagaatatg atcaaggagt aacaacacag gaaattgctg aagtgttagg gattcagcga
     6481 acaaatagca gtaaagatct gaatcagcta gtgcgcgaag gaaaactgat aaagacagac
     6541 ggacgaccag tacgttacat atatcaaaca ctcgttacgc acgtaaagcc agttacaaaa
     6601 catgtagtca gttacaaaga aacgccggtc tctgaagaaa aaaatacact ccaaatagat
     6661 actaaggata tctttgcaaa aatcattgga gctaacggca gcatgaaaaa ttcagtggaa
     6721 caggcaaaag ctgcgatttt gtatccgcca agaggattaa attgtttgat tacaggacct
     6781 accggatcag gaaagactta ttttgcacac gcgatgtatc attttgcaaa agcgcatcat
     6841 gtaatagatg aggaaacgga actgattgtt ttcaattgtg ctgattatgc caataaccct
     6901 gagcttttga tgagtcattt atttgggtac gcaaaaggtg cttttacagg cgcagatgaa
     6961 gaaaaaatgg ggattatcga tcaagcggat ggcggtatgc tgtttttgga tgagattcac
     7021 cggttgccgc ctgaaggtca agaaatgatc ttttacttta tggatcatgg aacttacagt
     7081 cgtcttgggg aaacaacgaa atcccatgaa gcaaatgtac ggatcgtagg tgcgaccaca
     7141 gaagacccag gttcttctct attagaaact tttgttcggc gtatcccaat caatatcaaa
     7201 cttccggctt ttgaaaaacg tcctgcaaat gagaaagtcg atttagtaaa aatcatgatt
     7261 gcccacgaag caaaccgcac gcagcgaaag atttctttga cagaagatgt agcaaaagca
     7321 ttgatcggca gtgtgaccta tggaaatata ggacaattga agtcaaatat ccagttagta
     7381 tgtgcacgag gatttctaaa tcatatgcaa tcacccgaaa tctcgatcac aatcgatgat
     7441 ttgacagaag ggattcgtag cggattgatt caattagcaa gcaaccgcag tgcaatggca
     7501 gagctttcta aaatattgga gccaaagata actgtagcac caaatgatac gatcatgaaa
     7561 atccaatcgg actcctatga gttaccttat aatctatatg atatcatcgg agataaagca
     7621 gctttactga agtcggatgg tttggatcaa gaagcaatca atcatttcat ttctacagat
     7681 atcaacatcc atctgaagtc tttctataaa gaccatggtt tttcttttaa tgcagacagc
     7741 aaattagcag aatttgtaga tccgaaaatc atagaagtga ccaatcagat ttatgtaatg
     7801 gtcaaaagat ctttaccata tgagtttcag caaaacttta tttatgctat gagcttgcat
     7861 atcagttctt ttttgaaaaa aatcaactta ggagaagaac ggcatacgaa tgataacatc
     7921 cgtgagatgg cttttgattt tccagatgaa tatgctgttg cgaaagaagt gcgtagttat
     7981 atcgaaacgt attttcaagt ggagattcca gaaagtgaag actattactt gaccgttcta
     8041 ctcgtctcgt tacgggcaaa tcaagcaagc ggcagaatcg gtgtagtcgt tgcagctcac
     8101 ggaaacagca cagcaagcag tatggtccaa gttgtccaac agttgttaga tgcagagaac
     8161 gtacgagcag tagacatgcc tttggatatg gatccgaaaa cagcattgga ccgaattgaa
     8221 cgcaatgtac aagaagtaga tgaaggaagc ggggtcatac tcttagtaga tatggggtcc
     8281 cttgcgtcgt ttaattcgca aattcaaaga gacactggaa ttccagtacg tactgttgat
     8341 atggtaacaa cttctctagt tttggaaact gtacgaaagg tctcagtatt agggacagat
     8401 ctagatatgt tgcatgaatc attgaaaaat tttcgaggtt acgcagaaat atcttctgta
     8461 gaatctgttc aggaagcaaa acctattcat aagaaaaaag ctgttttggc tatctgcgca
     8521 tctggcgaag gtacagcaca aagaatcaaa gaattgatcg aacgagcggt aaacaagcgt
     8581 caagaaacag aattagaagt tctagctttg tcgattgtgg aagtgaaaga agaactgcca
     8641 gttatccaaa aagaatatca gatcatagct actactggga ttactgattt gaatatccaa
     8701 gccccattta ttcctttaga acgttttatt gatcaaaata tcgaagtcat tttggatcag
     8761 ttgctgatgg aatcagaatt ggaggaaact gaattcatct ccctagatga agagtcagct
     8821 aaaaatacgt gtgttgagtt tatctctgat aacttcatct ttattaatgg tagtaagtta
     8881 atcgatccaa tgtggcaatt ttctacacaa atctctcaga cgacgggaat cggcgatgaa
     8941 gaatatggat tcaaaatcaa cctagtgatg cacacagcag gaatgatcga acgtattatt
     9001 cggaatgaac cattgactgt ggaagaaaac gaattgacaa atacaacgaa tgatcctctt
     9061 tatagccaat tggcagcctc agttgttctg ttggaagatc agataaaagt aaaagtgccg
     9121 atagaagaaa tgtattatct gctacgtcta gtccataatc agttagataa aaaagaatac
     9181 actgtgccat gaatacacaa aaacgataca cttttatagt gtatcgtttt tgtgtattgg
     9241 ttaagaatcc tttaaattca attagtgtat cgtaaaaaag ttggcacgct ttttgcttat
     9301 agtagtgtgt gctaggaggt ggttgttcac acagaaatga taggggtttc tgtgaagtag
     9361 tgaggaagaa ggagttttgc agatggatat tcgattggca cgtatagatg atcgtttaat
     9421 ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt gttgaacgta tcattgtgat
     9481 cagcgatgta gtcgctcaag ataaattgag aaagtttcta ctaaaagaag ctgcaccgcc
     9541 ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt caaatctatc atagtggatt
     9601 attatcacga gcaaaagtga tgcttttatt caccaatccc caagatgttg aagcggttgt
     9661 ccgctgcggt gtacagttga agtcgttgaa tatcggcggt atgagtttta ctgaaggcaa
     9721 atcaatgatt accaattttg tttcagtaaa tcaacaagat attgattcct ttcgttactt
     9781 gaacagtcaa gaagtggagt tagagattcg aaaagtacct gctgacagga aaatcctttt
     9841 gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa tggttgttgg aaaaggtcaa
     9901 ataattctgt tgtaatggtt agcaattatt aatatttttg aatttgttac aacaaaagta
     9961 aataggaggt ataaatatgg tagggattat cctagcaagt catggacaat ttgctgaagg
    10021 aatcttacaa tccggttcaa tgatctttgg cgaacaagaa aacgtcaaag ccgtgatttt
    10081 gaaaccaagt gaaggtccag atgacttgag agccaaattg gaagaagcgg ttgcttcttt
    10141 tgataatcaa gacgaagtgt tattcttagt tgatttatgg ggcggaacac cgttcaatca
    10201 atcaaacaca ttattcgaag agcacaaaga taaatgggca atcgtcagcg gtttgaattt
    10261 accaatgttg attgaagcct acgcatcacg cttttcaatg gaatctgctc atgaaattgc
    10321 agcacacatc attgaaacag caaaagacgg tgtaaaagta aaaccggaag aattagaacc
    10381 ggcagaagca ccgaaagcag cagttgaaga cgcacaacct aaaggagcac ttccagaagg
    10441 aactgtggtc ggagacggaa aaatcaaata tgtattagca cgtgttgact cacgtctgtt
    10501 acatggacaa gttgcgacag cttggacaaa agcagtgcaa ccaaaccgta ttatcgttgt
    10561 ttcagatgca gtatcaaagg atgatctgcg taagagacta atcgaacaag ctgctcctcc
    10621 aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc gaagtagcaa aagaccctcg
    10681 ttttggaaat accaaagcat tgcttttatt tgaaaatcct gaagacgtat tgacagctgt
    10741 tgaaggtggc gtagacatca aagaattaaa tgttgggtca atggcgcatt cggtcggtaa
    10801 agtcgttgta agtaaagtat tgtctatggg acaagaagac gttgaagcgt ttgagaagct
    10861 tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct aatgattcac gcgataacat
    10921 ggatgatatt ttgaaaaaag caaaagctga attagcaaaa gcgtaataaa taatattaaa
    10981 atttatagga ggcttatcat gtctattatt tcaataattt tagtcgtatt agttgccttt
    11041 ctagctggta tggaaggtat tcttgatgaa tttcaatttc atcaaccact agtggcatgt
    11101 acattgatcg gtttagttac cggtaacctc gaagctggta tcgtacttgg cggtacgctt
    11161 cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg tagcaccaga tgctgcatta
    11221 gcatcagtag catcagcaat tattttagtt ttaggtggac aaggtgttaa aggtgttcct
    11281 tctgcgatcg cgattgccgt tcctttggct gttgcagggt tgttcttaac aatgatcgta
    11341 cgtacaatcg ctgttccaat cgttcatttg atggatgcgg ctgctgaaga aggaaacatc
    11401 cgtaaggttg aaatgtggca catcattgct gtatgtttac aaggggttcg tatcgcggtc
    11461 ccagcagcag cgttgctatt tatcccagca caaactgtgc aatcattcct tgaatcaatg
    11521 ccagcatggt taacagatgg tatggctatc ggtgggggaa tggtcgtagc tgtaggttac
    11581 gctttggtaa tcaacatgat ggctacaaaa gaagtatggc cattcttcgt tattggtttc
    11641 gttgtagctg caatttctca attaacattg attgctctag gtgctttagg tgttgcatta
    11701 gctcttattt acttgaacct ttctaaaatg ggtggttcat caaataatgg aggcggaagc
    11761 aactctggtg accctttagg cgacattttg aatgactatt aatcttgaag gaggaaaata
    11821 aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg tttagctgtt gcgtggcgct
    11881 ctaccttcat tcaaggttca tggaactacg aacgtatgca aaacggtggc tgggcatttt
    11941 ctatgattcc agcaatcaaa aaattataca aaacaaaaga agaacgttct gcagcattaa
    12001 aacgtcactt ggaattcttt aatacacacc catacattgc ttctccaatt ttaggggtta
    12061 cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt tgatgatgta gcgattcaag
    12121 gggttaaagt aggtatgatg ggtcctttag ctggtgttgg tgacccagtg ttctggttta
    12181 cagtacgtcc aatgctaggt gcactaggtg cttcattagc tatgggagga aatatccttg
    12241 gaccaatcat cttcttctta ggttggaaca ttattcgttg ggcattcatg tggtatacac
    12301 aagaatttgg ctacaaagct ggttctaaaa ttactgatga cctttctggc ggattgttac
    12361 aagacgtaac aaaaggtgct tcaattttag ggatgttcgt attagctgcc cttgtacaaa
    12421 gatgggtatc aatcaaattc ttaccagttg tatcaactgt aaaattagat aaaggtgctt
    12481 acatcgaatg ggataaattg ccttcaggtg gtgaaggaat caagagtgca tttgaacaag
    12541 ttaataatgg cttagccctt tctcctgaaa aggtaacaac attacaagac aacttagacc
    12601 aattgattcc tggtttagct gcattaggat taacattgtt atgtatgtgg ttgcttaaga
    12661 aaaaggtttc tccaatcgtt atcatccttg gtttattcgt agtcggtgtt gttggacacg
    12721 tcatcggact tttataatcc ataaaaatat agctcgggcg ttgtcccgag ctttttttat
    12781 atagtatact gttcataaac aaatatctgg agggagaaaa tggttcaatc aatcaataca
    12841 aaagtagact tggtaattga tgctacttcg tttacgggtc tgacagatta cgggcaaatc
    12901 atgataggta ataatggttt tgagttttac catgcgcgag atagacgcaa atatatccaa
    12961 attccatggg aagaagtgga ttatgtacta gcatcagtca tgctaaaggg aaaatggatt
    13021 cctcgatatg ccattcaaac gaaaaaaaat ggtacataca cattttcttc caaagaacct
    13081 aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag atagaatggt acaatcattg
    13141 agtttctttg atgtagtgaa acgtggtatt cgtagtatgt ttaaacgcaa gtaaaaaata
    13201 aagaaaactt ctaagaggag gttttcttta tttttttact ttgcagaaga aaatatgaaa
    13261 tagcaaaacc aattatttgt cccctttaaa tcccatatgc taaaattgat tagcaaagaa
    13321 ggaggttata ttatgagttg gttatggaca ttgattgttg gagccgtgat cggagcaatt
    13381 gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg taacgaatat cattgctgga
    13441 ctagtcggtt caagtattgg acaagcgatt ttcggcgaat ggggaccaaa agctgcaggt
    13501 atggcactta ttccatctat tttaggcgca gtgattttgg tagcagttgt ctcatttttc
    13561 tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg cagcgagact
    13621 gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga aatttttatt
    13681 taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa agaaagaggg
    13741 agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga agaaaatttg
    13801 gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt tacaggtacg
    13861 acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt tcgttatacc
    13921 gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg acctattttt
    13981 gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt tgaggaaaca
    14041 acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga attagtacct
    14101 gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat cgctattatc
    14161 gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat gatccaaccg
    14221 ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc tcttggcgct
    14281 tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat gccgcatggt
    14341 gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt tggttgctat
    14401 tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc tggggaaaaa
    14461 ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga tgctgcaaaa
    14521 ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc aaaacatggt
    14581 tacggggaag catttggaca ttctacagga catgggatcg gtttagagat ccacgaagga
    14641 ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat cactgatgaa
    14701 ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt gttgatcaca
    14761 gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt atagtttcat
    14821 caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga aaatcactcg
    14881 atttaatgat acactataga agaactttta agctgtaaga gataataaag ttgggtgttt
    14941 tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac gtgatcacta
    15001 tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg aataaataaa
    15061 tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag atcgtgatcg
    15121 ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa ggcgtatacg
    15181 gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca gctcacggaa
    15241 aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac tgttatctag
    15301 aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc agccagcaag
    15361 tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta gctgttgttc
    15421 ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag gaagagaatg
    15481 aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt atttccatta
    15541 gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga attagaccac
    15601 cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga tacgctagtt
    15661 ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca tttaaaaaac
    15721 aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat cgcgattttt
    15781 gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc aatcgagttg
    15841 gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt atccaatgta
    15901 ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt tctatgaatc atcataaacc
    15961 tgttataaat cgggaaaatt ctatggtaga atgaaaacac aaaaaatatc tgggagtggt
    16021 tagatggcag aattgattaa cggaaaagaa ttagctgaaa aaatgcaggc agagattgct
    16081 gaaaaaatcc aaaaattgaa agataatggt atccatcccg gattagtcgt tttattggta
    16141 ggggaaaatc cagccagcca aatctatgta agaaataaag aacgtgtagc aaaagaaatc
    16201 ggaatccatt cattggtaga gcgttatcct gagacgattt ctgaagacga actacttgca
    16261 gaaattgaaa aatacaatca agatcctagt tttcatggta tattggtcca attaccattg
    16321 ccaaagcata tagatgaaga aaagatcctg ttagctatcc gccctgaaaa agacgtagac
    16381 ggttttcatc cgatgaattt aggcagactc cttgcgggta aaccggacaa gattccttgt
    16441 actccatacg gcatcatgaa aatgtttgaa gcttattcta tcgatccggc gggtaaacga
    16501 gcagtagtta tcggacgaag caatatcgtc ggtaaaccga tggctcagct actcttgatg
    16561 gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa atctggctga actggcacaa
    16621 gaagcagata ttttagtcgt agcaatagga cgtggacatt ttgtcacaaa agaattcgta
    16681 aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg atgctaacgg taagctgatc
    16741 ggtgatgtga aatttgatga agtggagcct attgcaggct ggatcactcc tgtgccaaag
    16801 ggtgtagggc caatgactat cacgatgctg atgtatcaaa ccgtaaaatc tgccgaagct
    16861 tttggagcag gtgaatagat ggcacaagaa tatttaactg tcactacatt aacgaagtat
    16921 ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg tatatttaac aggcgaaata
    16981 tcgaatttcc gtttacgtgc caatgcccat caatatttca gcttaaaaga tgaccatgca
    17041 aaaatatctg ctattatgtt caaaggagca ttccaaaaat tacgattcca gccaaaagaa
    17101 ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg aagcgggagg ttcttatcaa
    17161 atctatgtag agcacatgga gcctgatgga ataggtgctt tatatcaaga attggaagaa
    17221 cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc ctaaaaaaat gctgccacgt
    17281 ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag cggttatccg cgatatcatc
    17341 acgacggtga aaagaagata tccaattgtc caattggttt tgttcccaac agttgtacaa
    17401 ggaaaccaag cagcggacaa tgttgtcagg aatatccaga gagtagaaga gatgggcaat
    17461 ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg aggatttatg gccgtttaat
    17521 gaagagcggg ttgctcgggc gatttatcaa gctaatacgc cggtgatttc atctgtagga
    17581 catgaaacag ataccacgat cgctgattta gtagcagatg ttcgggctgc cactccaaca
    17641 gcagctgcag aattagctgt tcctgttttg tcagaagagt tgatgcgcat tcaagaacgg
    17701 caagctagat tgcagcaggc gtttgtacgc cagatccagc gcaaacaaga acggttcgag
    17761 cgtgctcgac gttcctatgt atttcggcag cctgaaagat tgtatgaagg tcagaccatc
    17821 aaattagacc agttgaaaca gcgattatat caagctacac agcaaattta tcatgcgaaa
    17881 gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag tggctccgct ttatcgagtt
    17941 aaagcagctc gccaagaaac tgcttatcta gaaaaacgct tagaagagaa aatccaacaa
    18001 tatatgaaac aacagcagca gcgttttgct caagctgttc aatctcttga tttgttgagt
    18061 ccattgaaaa tcatgggaag aggctacagc tatacgacgt taaatgatca agtagtaaaa
    18121 agtgtcaccg agattcatca aggggatttg ctgacgatcc attatcaaga tggaatcgtt
    18181 aaaggacaag tacaagagat agacaaagga gaataaaaat atggaaaatc caacttttga
    18241 agagtcactg caagaattag aaaaaattgt catgcagtta gaacaaggag acgttccatt
    18301 agaatcagct ctagattcgt ttaaacgagg aatggaatta agcaaacatt gccaagatac
    18361 attagctaaa gcagaagaga ccttgacgaa aatgatgaca gaatcaaatg aagaagttga
    18421 ttttgacgga aacgaggaaa gctgagatga gattaactga cttttcagct attcatctac
    18481 ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca tacaacggat agaggactct
    18541 atgatgcgat gagttattca gtgaaagcag gcgggaaaag aatacgtccc ttattacttt
    18601 taacggccgt tgcttccttt gatgagccta ttgatgttcc cgtctatcaa gtagcagctg
    18661 ctttagaaat ggtgcatacg tattccttga tccacgatga tttgcctgcg atggacaatg
    18721 atgatcttcg tagaggaaaa cctaccaatc ataaagtatt cggcgaagca ttagctatat
    18781 tggccggaga tggtttgctt acaggagcat tccagctaat cagtatggca cacttaggta
    18841 attctcctaa gcttctgctg cttcagcaat tagctgtttg tgcgggatca caaggtatgg
    18901 ttgctgggca agctgcagat atcgagggcg aatcgaaaaa gctttcttta gaagaattag
    18961 catttattca tgaaagaaag acagggcatt tgattcgtta tgccttgctg gctggtggca
    19021 tattagcgaa gcaacctgaa gaaatcttat tgctacttca acgattagcc gaacatttag
    19081 gtttagcttt tcagataaga gatgatctac tcgatgtgat tggtacaacg aaaacattag
    19141 ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac ttacccgcgt ttattaggcc
    19201 tagaaaagac acgtgaggcg ttggagattg agctgttatc cgccaacaag atcatagaca
    19261 aattagaaga ggacgttttt tcttttgatg gcgggctgat gcgccagatg atcaagcaat
    19321 ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc gtagatgtat tagcggtcaa
    19381 acaaggatta tttgaaacaa gagaacaagc caaacgttca gtgatggctg gtttgattta
    19441 caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa attccagtga gtagtgaatt
    19501 aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg ggattaaaat tagaaaaagc
    19561 actcaaagca tttgatctat ctgttgaagg aaaaacattg ttagatattg gtgcatccac
    19621 tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa atgagttatg ctttagacgt
    19681 tggctacaat cagctagctt ggaaaataag acaagatcct agagtcgtcg taatggaaag
    19741 agtgaatttt cgttatgcaa aacccgaaga ctttacctta ggaattccag aagttgctgt
    19801 tatcgatgtt tcttttattt cactaaaact gatgctgcct ccattacatg ctattttaaa
    19861 agaagaggga gaagtgattg ctttaatcaa accccaattc gaagcaggca gagaagctgt
    19921 agggaaaaac ggaattgttc gtgaccctca gacccatcaa aaagttttag aagacatttt
    19981 gtcatttgcg gcacaaggtg gatatgatgt gctggaatta agttattctc cgatcacggg
    20041 gggagaagga aatatcgagt tcttggctca cttgaggaaa gtacccgaaa gcggaacaat
    20101 caattcagct attaatatgg cagaagtggt ttcaaatgca cacgaacagt ttgaccataa
    20161 ataaattgta actgtgaaaa tcaagtgaga agtatgcgga ttcttcttta ttataccaag
    20221 gaaataagct atcattgatt aagaagaata tgctaaggag cgaagacgta atgagaaaaa
    20281 aagatcggca tcgtttgatt actcgattgt tgagtgagca agatatccga aagcaggaag
    20341 aatttgttga aatcctgaaa agcaaaggaa tatctgttac tcaagctacg atttccagag
    20401 atataaagga actgaaactg atcaaggtac cggcttcaga tggcggctac cgttatagca
    20461 taccagctga aacaaacgaa gatgtaaata ctaaattgga aaaactatta aaagacgctt
    20521 tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa aacattgccg ggaaatgctt
    20581 cggcagcagc aaatttgatt gataaacgtt acaagaaaga attattttcg attatcaatg
    20641 atgacgataa tgttttgatg attacacgaa ctgaagacca ggcagtttta ttgaagaaag
    20701 acttcctgca ttacctttaa gttgaggagc gaatgtgatg ctacaagaaa tcagtattac
    20761 taactttgcg attattccgg aattgcggct ttcttttcac gaaggaatga ccgcgctgac
    20821 cggagagact ggtgcgggta aatccattat tatcgatgct ttaggtctgt tagcaggagg
    20881 aagaggttca agcgattata tacgtcaagg agcagaaaaa tgtgtgcttg aagggttgtt
    20941 tgaactacca aaacaagaag gattctctga actgatggtt gaattaggga tcgaaacaga
    21001 tgaggacaat ctgattgttc gacgggatat gtccttaact ggaaaaaatg tttgtcgagt
    21061 gaatggtcat attatcacat tagctaatct gagaagaatc ggcagttatt tagtagatat
    21121 ccaaggacaa aacgaacatc aggaactgct ccagcctgaa tcacatcttg ctttattgga
    21181 tcgttttgga gatgtagctt tccaacagaa gaaaaagagt tatcagcaag aatatatctc
    21241 ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa aatgaaaaat cctatgttca
    21301 gagaatagat atgctccatt tccagcaaga agaaatagct gctgctgatt tgcaagtcgg
    21361 agaagaagag aaattaaaag aagaaagaga aaaacttagc aattatcaaa aaattgctga
    21421 tggattagct gcaggttacg gagcactgac gaataatgaa caaaacagtg tagatggtgt
    21481 tggcttggcc gtttcagaac tgcaagggat cgcccatcta gatgtggagt atgaagcaat
    21541 ttatgaaaat atccaaagtg cttactattt gctgcaagat gcaatcggcg atatgagtcg
    21601 tcagattgat ttattggagt tagatgagag tcgtttagaa gaagttacgc aacgtttaga
    21661 attaatccga cagttgaaac gtaaatacgg cgaatccatt gagtcaatat tggcttacta
    21721 tgacgaaatc accgaagagc tagcttcttc tgatttttca gaaggtcaat tggacaaaat
    21781 gaaagaggaa ctagagcaaa aagagcttct acttcagcaa caagccgcag atcttcatga
    21841 agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt ctgcatgaat tgaaaagtct
    21901 gtatatggaa aacactgagt ttgaggtacg tttcttaaaa gaagaaaata gacaattgaa
    21961 tcatgatgga tttgaccaaa tcgaatttta cattacgacc aatccaggag agccactgaa
    22021 gcctttggtg aaagtcgctt ctggtggcga actgtctcgg atgttgcttg ctttgaaaac
    22081 tattttttct tctgagcaag gtgtgacaag tattattttt gatgaagtgg atacaggtgt
    22141 gagcggaaga gttgcacagg ccatcgcaga taaaatttct caaatatcga aatactctca
    22201 agttttgtgc atcactcatc tgcctcaagt tgctgcagtc gccgactatc aatattatat
    22261 tgtgaaagca gtgattgatg gacgtacgca aacttctgtt tcagagttga aaacaaaaga
    22321 aagagaagaa gaaattgcac gtatgctggc tggaagtgag attacaaaac tcacactcga
    22381 acatgcaaaa gaactattga aactcgcaaa aaggacctga gccacctcgc agtggttcag
    22441 gtccttttca gcagctgata gctatatttt atttttacat gacactcatt acagcagtca
    22501 atacaacaga tccaatcgtt aaaatcagca ttgtccaaat gacaaatttt gttactttag
    22561 caaaagtact tgtttgtttt ttttcacgca ttgttttcaa cctcgtcatt aattagttga
    22621 ttcaagtgta caatcttttt tccatttcca caagagggct tttttataaa aacttccctt
    22681 tttttaaata ataggtagtt acgatacaaa taaataatgt taatagaact agccatataa
    22741 aggcatcatc cctttcagca ataggtaact taatgttcat tccatagata ctgccgacga
    22801 ttgtcggtat agttaaaata atcgttaaag aagttaaaat tttcataata ttattcagat
    22861 tattcgatac gattgctgaa aaagtctgat tcatttgttc tactagtttt aattgaatgc
    22921 gagaagtagt cattgcctgc tctgtttcca ccaatacatc acgtaaatga tttttcaaag
    22981 catgattttc ttggaaaagt ttattttgcg ctagttcttt caatgcatct aaattagcag
    23041 aagtcgcttc tctgaaataa acgaggcttt tttggatatc catgatttga taaagttgtt
    23101 tgttttcagt ggatacttga agtttggctt ccaaagtatc gcattgagat tgaagcgtag
    23161 ctaaataact gttgtaagag agtgtgacct gccaaatgaa ttgaaagaag atattgagat
    23221 taggcgaaat atcacttttt ggaaaggtct ggctaaagac tttctttaaa aacagtggtt
    23281 catgattcgt aattgtgata agcttctttt tgggcgtcaa aataatagag agcgggtatg
    23341 tatccacttg aaaataaccg ctaggacttg ttttagcaaa aggatattgt aacaataata
    23401 acgctggctt tgtcaacttt gattgattga attcttctgt acgggaattt tctgcatcgt
    23461 ccaatatgga agtgatgtaa tctgacggaa tttcaaattg atgtgtcagc tgatgaatct
    23521 cttcttgatt aggacgttct acacaatacc agatcgtttg gtcagtttcc tttccggtat
    23581 tacgcagttt ctcattatct aatgtaaaat atttgatcat gattttcctc ctgttgcctg
    23641 tttcttttat tataacggat agggaattgg aatacatgga atgtgtttgt aagatataga
    23701 atataactgc ataaatttaa ataaaatgaa acagttgtgt aaagtatata cagtatactt
    23761 gaagcaacga taaaaaagga gtttacgttc atgggcacat tcacaccact tatcttgttt
    23821 tggtatattt acccggtaat cgttttgttt ggttgccaat ttcttgtaaa attattttcg
    23881 ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt gtggggcggt
    23941 atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt ctttatttct
    24001 gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga agaaattatt
    24061 tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat cgtcttgtac
    24121 gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa aagctgtaaa
    24181 aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg aataaaaaaa
    24241 gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt agactaattt
    24301 tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac ataatattga
    24361 tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt
    24421 tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct
    24481 ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt
    24541 cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag
    24601 cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg
    24661 aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt
    24721 tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta
    24781 atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac
    24841 atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat
    24901 ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca
    24961 atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc
    25021 aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc
    25081 attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt
    25141 tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat
    25201 cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag
    25261 gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc
    25321 caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga
    25381 aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa
    25441 ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga
    25501 atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa
    25561 tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga attccaacca
    25621 gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac
    25681 aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag
    25741 aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga
    25801 tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc
    25861 accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc
    25921 gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc
    25981 tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag
    26041 tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga
    26101 aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa
    26161 taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt
    26221 aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc
    26281 gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt
    26341 gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt
    26401 ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac
    26461 ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct
    26521 gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa
    26581 aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa
    26641 gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca
    26701 tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc
    26761 agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat
    26821 cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc
    26881 aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga
    26941 cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc
    27001 agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca
    27061 acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg
    27121 taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc
    27181 agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca
    27241 agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg
    27301 tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg
    27361 acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga
    27421 tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta
    27481 tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa
    27541 taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga
    27601 agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa
    27661 agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg cttatggtgt
    27721 atatagtgta cctggataca atgtatcggc aaaaaacgga acagcccaaa tcgcttctga
    27781 tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc
    27841 ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg
    27901 aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac
    27961 tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa
    28021 tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat
    28081 cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg
    28141 agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc
    28201 aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga
    28261 tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag
    28321 ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag
    28381 cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg
    28441 gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca
    28501 atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga
    28561 caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt
    28621 ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga
    28681 aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca
    28741 tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag
    28801 atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa
    28861 atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt
    28921 ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct
    28981 taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt
    29041 ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc
    29101 taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg
    29161 tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa
    29221 ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt
    29281 tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa
    29341 atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc
    29401 ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga
    29461 aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata
    29521 acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca
    29581 gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt
    29641 acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg
    29701 aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa
    29761 ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca
    29821 aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat
    29881 ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt
    29941 acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat
    30001 tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca
    30061 cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac
    30121 tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag
    30181 cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat
    30241 gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta
    30301 ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact
    30361 aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac
    30421 agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta
    30481 tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata
    30541 gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt
    30601 gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact
    30661 agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg
    30721 ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct
    30781 tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc
    30841 aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga
    30901 gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct
    30961 taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg
    31021 tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac
    31081 agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt
    31141 ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt
    31201 tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa
    31261 aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc
    31321 acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca
    31381 agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga
    31441 aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag
    31501 tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt
    31561 accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat
    31621 gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc
    31681 actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg
    31741 agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca
    31801 aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg
    31861 tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat
    31921 ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca
    31981 cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag
    32041 tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat
    32101 aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt
    32161 cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat
    32221 gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc
    32281 caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt
    32341 catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat
    32401 atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct
    32461 ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa
    32521 aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa
    32581 atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct
    32641 gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac
    32701 ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa
    32761 gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat
    32821 ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt
    32881 gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa
    32941 acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt
    33001 caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac
    33061 tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt
    33121 gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga
    33181 atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata
    33241 ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta
    33301 ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata
    33361 aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga
    33421 tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa
    33481 tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt
    33541 cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag
    33601 attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat
    33661 tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat
    33721 gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg
    33781 aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg
    33841 gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag
    33901 aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg
    33961 cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag
    34021 aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt
    34081 cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc
    34141 aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc
    34201 ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa
    34261 atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag
    34321 caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag
    34381 ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg
    34441 aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa
    34501 tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa
    34561 ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt
    34621 ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt
    34681 acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta
    34741 ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg
    34801 gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc
    34861 actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg
    34921 aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg
    34981 aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta
    35041 ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa
    35101 gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc
    35161 acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa
    35221 ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg
    35281 aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt
    35341 cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc
    35401 gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa
    35461 atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa
    35521 cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta
    35581 ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg
    35641 gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc
    35701 gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa
    35761 ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc
    35821 tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga
    35881 tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga
    35941 attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa
    36001 gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg
    36061 cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag
    36121 attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa
    36181 tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga
    36241 atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga
    36301 agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt
    36361 tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga
    36421 ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa
    36481 agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt
    36541 tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg
    36601 ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg
    36661 cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc
    36721 agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga
    36781 aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca
    36841 ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg
    36901 gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg
    36961 attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga
    37021 tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag
    37081 atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg cactattact
    37141 tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct
    37201 ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga
    37261 accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat
    37321 tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg
    37381 gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata
    37441 ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt
    37501 tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac
    37561 cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga
    37621 tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt
    37681 ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta
    37741 tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg
    37801 gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat
    37861 tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt
    37921 acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga
    37981 aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc
    38041 cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa
    38101 tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag
    38161 agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg
    38221 tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc
    38281 caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc
    38341 ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa
    38401 gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac
    38461 caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa
    38521 tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa
    38581 aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat
    38641 gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga
    38701 gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg
    38761 gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc
    38821 aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca
    38881 ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg
    38941 cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg
    39001 atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct
    39061 tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca
    39121 ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac
    39181 atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa
    39241 accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc
    39301 gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa
    39361 ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta
    39421 ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc
    39481 tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga acaagtattg
    39541 gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt
    39601 gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca
    39661 ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc
    39721 gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg
    39781 tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct
    39841 ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca
    39901 gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta
    39961 aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa
    40021 gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa
    40081 tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca
    40141 cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct
    40201 ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca
    40261 cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat
    40321 gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta
    40381 gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg
    40441 gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta
    40501 gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac
    40561 cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat
    40621 gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt
    40681 gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga
    40741 tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc
    40801 tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc
    40861 ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc
    40921 acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt
    40981 gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa
    41041 aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat
    41101 gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa
    41161 atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat
    41221 actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa
    41281 gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt
    41341 acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat
    41401 gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat
    41461 caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa
    41521 atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat
    41581 gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt
    41641 gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg
    41701 gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat
    41761 gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat
    41821 gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca
    41881 gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa
    41941 cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta
    42001 gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac
    42061 ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca
    42121 gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc
    42181 agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac
    42241 gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa
    42301 cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt
    42361 tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa
    42421 ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt
    42481 cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa
    42541 tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg
    42601 aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc
    42661 tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc
    42721 accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg
    42781 gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg
    42841 cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag
    42901 tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt
    42961 aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa
    43021 acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat
    43081 atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc
    43141 agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg
    43201 tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac
    43261 acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg
    43321 ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt
    43381 tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg
    43441 ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc
    43501 ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc
    43561 gccgaatatc gtaaacaata catttttttc attagacatg tggatacact cctttatatt
    43621 ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt
    43681 tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata
    43741 ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata
    43801 cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact
    43861 tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt
    43921 tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg
    43981 acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg
    44041 cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg
    44101 gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct
    44161 gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt
    44221 tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg
    44281 gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat
    44341 aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact
    44401 cattctttcg tttataaagg atatatatat acattgggag ggatctattt ttcagataac
    44461 aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata
    44521 agataaaaac catactgatc acgtacgact aaacgatatt catctatttc taccacagat
    44581 gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt
    44641 ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt
    44701 tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca
    44761 agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag
    44821 acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt
    44881 tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag
    44941 gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg
    45001 gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac
    45061 aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg
    45121 acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa
    45181 tatcgatagt caattgccta atcggctggc aattgatggt attttagacg gcacgatcct
    45241 tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc
    45301 caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg
    45361 tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt
    45421 gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt
    45481 tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact
    45541 agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca
    45601 agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt
    45661 tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac
    45721 aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac
    45781 agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga
    45841 tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta
    45901 tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa
    45961 aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca
    46021 tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg
    46081 ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag
    46141 ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt
    46201 ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc
    46261 tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg
    46321 tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc
    46381 aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt
    46441 gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat
    46501 cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc
    46561 caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga
    46621 gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt
    46681 cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat
    46741 tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt
    46801 tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa
    46861 acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg
    46921 gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg
    46981 ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag
    47041 attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa
    47101 ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca
    47161 gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca
    47221 acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa
    47281 aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa
    47341 ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt
    47401 ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc
    47461 gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag
    47521 aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt
    47581 ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat
    47641 gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg
    47701 ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg
    47761 cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa
    47821 actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact
    47881 ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa
    47941 aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct
    48001 atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc
    48061 aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag
    48121 ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg
    48181 cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt
    48241 caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg
    48301 gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac
    48361 cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg
    48421 aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt
    48481 ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga
    48541 tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag
    48601 acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc
    48661 tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg
    48721 aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt
    48781 tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg
    48841 agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta
    48901 tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg
    48961 gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc
    49021 atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat
    49081 tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt
    49141 atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca
    49201 tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga
    49261 acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca
    49321 aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa
    49381 ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc
    49441 gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga
    49501 tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa
    49561 tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg
    49621 gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca
    49681 aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg
    49741 cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag
    49801 ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga
    49861 atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg
    49921 aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag
    49981 cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt
    50041 cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta
    50101 aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc
    50161 gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac
    50221 gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg
    50281 cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg
    50341 atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa
    50401 ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt
    50461 cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag
    50521 tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa
    50581 gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata
    50641 tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg
    50701 atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat
    50761 catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga
    50821 aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa
    50881 aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg
    50941 atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa
    51001 gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc
    51061 gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt
    51121 gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga
    51181 gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt
    51241 ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg
    51301 gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt
    51361 tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa
    51421 cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc
    51481 acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg
    51541 aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa
    51601 aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta
    51661 taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt
    51721 tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcat
    51781 actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt
    51841 accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc
    51901 cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc
    51961 cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga
    52021 tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc
    52081 attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc
    52141 tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa
    52201 actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt
    52261 cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc
    52321 accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat
    52381 tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt
    52441 gcaaaaatat attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat
    52501 cgtcacagta tttgttcaag tattctggtt ctttggtatt catggatcaa atgtattggc
    52561 accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa
    52621 aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc
    52681 ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac
    52741 gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc
    52801 taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc
    52861 aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc
    52921 aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg
    52981 ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat
    53041 cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc
    53101 aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc
    53161 tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca
    53221 gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga
    53281 cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc
    53341 cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc
    53401 tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga
    53461 taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc
    53521 cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag
    53581 gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat
    53641 ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc
    53701 tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa
    53761 tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt
    53821 cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca
    53881 ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga
    53941 gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt
    54001 taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga
    54061 aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa
    54121 gatagatctt tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca
    54181 aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat
    54241 gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat
    54301 tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc
    54361 ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct
    54421 tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa
    54481 gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat
    54541 cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat
    54601 gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa
    54661 gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc
    54721 gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat
    54781 tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt
    54841 attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta
    54901 agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat
    54961 caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg
    55021 aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca
    55081 tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta
    55141 acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt
    55201 cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg
    55261 gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg
    55321 ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt
    55381 tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct
    55441 cttttttcgt aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt
    55501 cttggattgc ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg
    55561 cgtatacttt ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt
    55621 ctacctctag tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct
    55681 gattttttta gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga
    55741 aattgttcct gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt
    55801 caattccagt caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt
    55861 tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca
    55921 gtattgattt tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac
    55981 attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct
    56041 tgataaagta aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca
    56101 atccctaatt cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat
    56161 ccatttttta agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat
    56221 aattcttttt gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa
    56281 attttcctat ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat
    56341 gttttattaa tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga
    56401 agttcagcaa atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac
    56461 tgcgcagaaa ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa
    56521 tatttatctt cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga
    56581 tcaaacggaa ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta
    56641 aacttaataa cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat
    56701 cccactctat ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac
    56761 gtatcttcgt attttctata agtgatagga gatactgttc cttctttata aagttgcatc
    56821 caatcctcaa agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc
    56881 aagttatatt ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat
    56941 tttcttatct tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta
    57001 tttccacgtt tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg
    57061 aactataaag aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc
    57121 ggcgcaagga tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc
    57181 aatcagaggt gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt
    57241 ttagattatt tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc
    57301 tagatgtcat attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa
    57361 atgagatatt ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat
    57421 cgttttttct aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg
    57481 tgaatagcca gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca
    57541 tgctaacaat tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc
    57601 gttatcccat gaagcgttgt taattcgaag taaacaattc ttataaagat ttttatacat
    57661 atctatttta ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc
    57721 aatgagttgg tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt
    57781 ttctaatgat tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt
    57841 tttagaccca aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc
    57901 gccttcaact attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat
    57961 agttacctcc tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt
    58021 gtataggttt tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt
    58081 tacttacttt actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc
    58141 ttgaactaat ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt
    58201 ttaagtcctt cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac
    58261 ttttatcatc gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa
    58321 cagttccttc aaaagtatat gttttaccac taatatattt agtaaaagct gtattttgct
    58381 cagtagctgg agtaatagaa taataactat ctaagaaagc accccagtca tgattatttg
    58441 tctctaaaaa ttcttcaaat tgagttaggc ttttattgct gctgttagat gtagtagttt
    58501 gagtatcaga tgatgagctt tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa
    58561 gcgagaaacc tacggtagta aaaataatcc cgataataag gagaatcata ggtgttttct
    58621 tatttgtttt tttgataaat cttacaatta acatgattaa acctataata aataaaacaa
    58681 taccagtaaa accaaatata ccgtccatta atagatcctc tttctctttt gatattgaat
    58741 attataaacc aagcaattct tttttcttaa aatcgaattc ttcttgatta ataatccctt
    58801 catctaacaa ctctttatac tttttaattt catctgcact tgaaatttgt gtttctttgg
    58861 aattattatc taatatagat tgttgcttct ctatatattt ttttaaattt tccatttcat
    58921 tccaatattt ttttgtaaac ataacggtgt tttcatcttt cgtggccgct aatatacctc
    58981 cgcgactttc gtttccgcct agcaaggtaa attgtatata accattactc atacctggtt
    59041 tttttaattg tacggctgat atatttttaa aggggatagt tttttctcct ttaatacctt
    59101 gattaactaa atttataaat ccttttctaa taattgaaat agaatcatca gttactttaa
    59161 tcaaggtttt tcctggagat tttattaaaa tctctttttc cattttttct tcctcatttc
    59221 tgtgatatga ttttttttgt agaagctcat atatgaggaa agagcccgtg ttgcagcacg
    59281 ggctttttta atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat
    59341 attgccttgc gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt
    59401 atctgagctt attctgaata gaataatact ccataaattt ttcgagatta aactgagatt
    59461 catcagtcag ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg
    59521 cttctgtttc aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa
    59581 aatgagaggc gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac
    59641 cgtatagatt ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt
    59701 tcatctccat ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat
    59761 acatacaaac catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc
    59821 tttacgcatt gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc
    59881 tgataaatcc acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga
    59941 gacatcgaaa tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca
    60001 ctttgctaga taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt
    60061 tttcctcttc gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct
    60121 aaatgtttac aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa
    60181 tgagctatac tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat
    60241 tgatatgtaa atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta
    60301 attatgctct tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg
    60361 ttaaatttgt ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt
    60421 taatttcgaa caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg
    60481 caaagacgtt gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt
    60541 agatttggaa gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac
    60601 aatcatcaac gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa
    60661 aaaatttaaa cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta
    60721 tctaactcca gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc
    60781 aactcaacta aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta
    60841 cgaacctaaa atctcctatt tagatagcat attatcttct acagattcag taacaattag
    60901 tcagattgca gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact
    60961 aggtattcag aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg
    61021 atacacaaaa tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat
    61081 gaataccaaa tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg
    61141 atattaccct caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac
    61201 attgcagaaa tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag
    61261 ttcttaactt acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta
    61321 attttaaacg gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact
    61381 atgtacgaat tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt
    61441 ccagaaggct atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat
    61501 actggtagat ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg
    61561 tttacttcta gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac
    61621 aaaaatggat ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt
    61681 aaaacacttg aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg
    61741 aaaatttaat agagcgctag tgttcagcgc accgctaatc atctacgctt taggactttg
    61801 gggaagcagg caagcgttga taggaacgat tgtttacatg gtttggattt ttatggggct
    61861 tgatgaagct gaagctgagt acagagcgaa aaagccaacc gagggggctg actaaaaatg
    61921 aatagaaaag agaaactaga atgtatatta ctattactca gtttaattct ttcactaatt
    61981 tctctattgg gaagtttgta tttttgatat caacaatttt gtggatttgg gttctattta
    62041 gagtaaatat catttttgtt tggtgattaa ggagtttatt aaaattaatt ttccctactt
    62101 caaatgcaag gtaacaagat atagcacttt taggaggaat atttagcggt acagcttcag
    62161 agtatattct attatcatcg actgatcctc ctttagcaat taatttctta aatggagtag
    62221 ctgaccatct gcggtttaag ttagcggatg aaagttctaa attaactaac atagctggtt
    62281 ctgttgaata gttagaaatg attacttttg tataaatcat atctgcagcg aagtaagaag
    62341 cattaagttc aacttctagt tgagggcgca ttccttttat ttttatccct gttaaaaacg
    62401 tactcaatat aaaacttatt attgacatcc attgaaaaat tgttaagtga aaaaaattca
    62461 aaataaacac caccagtttt taactaaatt ataccaaaaa ggagagaaga aataatgcaa
    62521 gaattagtaa ttttgaaaaa taaagaagct gtgactacga gcttacaagt ggcagaaagc
    62581 ttcgagaaaa aacatcaaca tgttttaaga gatattgatg cactaaaaaa agatgtgtcc
    62641 aattttggac agatgtttgt agaaggtaat gaaccagatt catatggcag aaatcgacga
    62701 gttttcttca ttagtagaga tggatttttc ttgctggcta tggggtttac aggaaagaaa
    62761 gctatctact tcaaacaaaa atacattgaa gcattcaacg aaatggaaga tgttattcgc
    62821 aagaatactg ttcctcaaac aattgaagat atgatgatct atcaattaga agaaatgaaa
    62881 gatgttaaaa aagatgtttc catgcttaaa gatactatgc gaattagcgg acaacaagag
    62941 tttgaaatta agcaaaaagg aaatatgaaa gttatgggag ttctaggggg aaaagaaagc
    63001 cgagcttatg aagaaatcag caaaaaagta ttctcaaaat tttggtctga atttaaacgt
    63061 accttttcaa tcccaagata tggcgagtta cctcgtaaga gattcgatga tgctgtttca
    63121 tttattgaaa tgtggttgcc agaaactgcg atccgtatgg aaattgatca actgaacaga
    63181 caacagagac tttttggtga tgacaatgaa tagagctgaa gcgctaagaa tagggacggc
    63241 aattgctaat tgctggtgga aatactataa accaatcatc ctaagccaac aacatattga
    63301 caagcaaaaa gcatggcaac aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa
    63361 agaaaacaca tcataaggag attttagcat atggaaaaag aactttccac tctagatcaa
    63421 tatttgattg atcctgattg gggcaagccg aaaattgagg aaacaagtgg tcgaaaaatc
    63481 agacgaaatc ttttgacgaa tgaagaacta gcttgggatc aagatgattt aggcaaccat
    63541 gtaactattt gggatcatgt ttatcttatc catctatcga agcattcgaa taaacctgaa
    63601 tatatttacg tcatcgaaga tggcttgatt gatgcgctag aagagtacga cagagataac
    63661 ttgattgata tctcttatta cggaccaggt aagaaataca ttgctgaaat ggaggcagaa
    63721 tttgatgagt gaaatcaaag ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa
    63781 taaaattctc aagaaaaaag gaaattttga ttatttatct tgggctcacg cgtgggagat
    63841 tatgaaaaag aatgatccac aggcaacggt aactattaat gagtacaaac actacagggt
    63901 tgtttctgga actcatcaag actttcttgt tgaggaatat aaaccttttc ttatggatga
    63961 aactgggact tatgtatctg tctcagtaac ggttaaagga cacacggaaa ccgaattatt
    64021 tcctgtttta gattatcgaa accaaccagt tgttaaacca aatgcaatgc aaatcaataa
    64081 ctcattgaag cgatgctttg tgaaagcatt ggctctacac ggactgggat tatatgtatt
    64141 tcaaggggaa gatattccaa caccacctag aatcgataca aagaaattaa acatgctaga
    64201 gacgattcta gaagctttca atgagcagat gggtaaagat atgaccaaaa ccttaatcga
    64261 atatgttaat gagcagacag ataaattagg gctcttagct gataacgttg aaactattga
    64321 acagttaagc tatgagcaat gtgccttgat ggagcgagca atagcagcta agagaaaaga
    64381 attagataag aagtgatatg agtgtttaaa ccattaatcg attcatattc agcggttctg
    64441 aaaaagttca aaggaaaaga cataagcgca accatcaatg aggaagtgaa cattgatcga
    64501 ctaaagacga tgtatgacgg ctacgatggt gatcgagtca ttgaaattcg ttttattgat
    64561 cctagacgtt tcaccgtaca gcaacgaaac ttcatctatg cgctgatagg cgatattttt
    64621 atcgatacag gcatgccaac ggacttctgg aaggaattct tctactttcg ttttgaaggt
    64681 gtcacagggc gcaaaataag cctgaaagac gaatcgaata caactgtgag tgatgccaat
    64741 gtcttagcaa atatcatctt agatttcatc tttgaacatc atattccttt caaagaaggc
    64801 tatgagattt tacctgcgaa tcaagaatat tacttctaca aatgcatcac aaaaagagtc
    64861 tgctgcatct gtggcaaaac aggagctgac atcgatcact ttgacaaagc gctaggaaga
    64921 cgaaagcgca aagaagttga tcatgcagag tacacatttg cagcactctg cagaatccat
    64981 cacacagaga agcacaaaat aggtgtgatt aatttcaaaa ataaatatca aatcaaaggg
    65041 atcaaattaa accagaaaac aatcaaaaag ttaaatattg gaggggaaaa atgacagaac
    65101 atcgaagtta ttatgcgatt ataccagcca acgtaaggta cgacaaaaga cttaaaccaa
    65161 atactaagtt gttatacgga gagataacgg ccttgtgtaa tgaaagaggc ttttgttggg
    65221 caggcaatga gtactttgca gatttatatg gtgtgaataa agagaccata tcgcgatggg
    65281 taagtgattt gattaagttt ggatacttga atcgggaaat catttacaaa gagggtacca
    65341 atcaaataat caataggtac ctacgaatta atcaataccc tattgacgaa aaacgcaata
    65401 cccctattga cgaaaaagtc aaagataata atacatctat taataataca tttaataata
    65461 caaaagaata tataagagag ttaccgcctt cgaaaaaatc gaaggctaag cccgtccgtc
    65521 ataaatacgg agagtataaa aatgttcttt tgtcagatga gcagatggag aaactcaaaa
    65581 cagaattccc taatgattac caagagcgaa tcgaacgact gtcagagtat tgtgaatcat
    65641 ctggtaagac ttataaaaac tatttggcaa ctattcgaag ttgggcaagg aaagaaaaaa
    65701 gtgaacctaa gaacgcaagc agtggataca agcgcacagg aagacgagag aagcttcctg
    65761 aatgggcaat cgaccaagaa gcctatctca agaaaaaagc gctagaacga gctaatagac
    65821 aatcaaaagc accattctaa gaggtggaaa aatgaagatc gattatctag aactaattaa
    65881 tgaaatagcg aattataaaa agggcgagga attagacgtc ctgagagacg tatatgatca
    65941 actcgaagaa gctggaattg aaggaattaa gaatgatcgt tcgagttgga gtaaactcag
    66001 atactatttc gcactctata tcgatggaac gcaattaaga aatttagcat ataccaaatt
    66061 actattcatt gattgtgtta aaggcttgca aaaacatctt aatgaacttg agcaggtgta
    66121 aacaagatgg acctaaagac atttacagca cagatcgaat taatgcatca agaagcttta
    66181 agacaaagtg tgtcgtacga agacaagtgg ctcaacacat ttcatggcgg acgtgagagc
    66241 gcacttgatc aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc
    66301 aatgcagcga attatcgaat tgacttattc agaagattgg caggaagata aaaaaatagt
    66361 tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc
    66421 gagaaagatt gcaatctggc atggtgatcg aattctagta acgggtaccg ctaaacagtt
    66481 atctgaaatt actggtctga gcaaaaatat tatttgggat agagctagga gcttatggat
    66541 tgattcaaaa ggaagacagt ttaggtatgt ggaggagaaa taatggacga actaatcgcg
    66601 aaagtagagc agtgggcaaa agataaggga ttggatcaag cggatttcag caagcaaatg
    66661 ttaaaaacgg tcgaagaaat cggggaagtt gccgcttctc tagctagaaa agatgaacat
    66721 ggtttaagag acggaattgg cgatgtagta gtaaccttga tcatcttggc aatgcaaaat
    66781 gatatggatt tatacgagtg cttggatttt gcttatgacg aaatcaaagg tcggactgga
    66841 aagatggtca atggtgtttt cgttaagtcc agtgacctga aataattcca ttaatggaag
    66901 aaataactga ataggaggat agacatgatt gatgcaaaac aagtgcaaaa acagaaagat
    66961 ggaatgttga tgtttgaagc atatgttcta ccatttctta atcaatttga agttttggaa
    67021 tgctctgcca gcggagaaga attggagtat gttgtaattc gtgaaaccaa agaaaacgtt
    67081 cagaagctaa acgaatttct ttgcactatt aactgttggg atatgatagc tccaggattt
    67141 ttatgtccag ctatgggaga atttttggaa tattgtcgtc ttgaagatgc aggcactttg
    67201 gatttagctt atctggtata caactaccta aacatcaaca cagatcattt gtggtttggc
    67261 actgccgaaa gaaaatgggt agtccgctaa ccaccaatat caatgaaaaa aggcggtttc
    67321 ctgaaagtgg aattgccgcc attgctgtaa ggttctttac gcgactcaag gtcacagaaa
    67381 tataaatgca aatctctttt gaagtgatcc aaagctctcg gaaaatggaa gttttttgtt
    67441 tcaaagaata aatgagattt gcgtcaagaa cctgagagtt atttccaaaa aagcttccaa
    67501 agaaccgaat gagctaagga catcatgtgt aaacacataa tatagctctc ctttcacggc
    67561 taatcataac atgaaaaata ttcgatgtca gtaggaaaaa aggtgaatta tgaaaagaat
    67621 attaaattat cccggcagta acagattctg caattaaact ccgaagctga tgtatcagta
    67681 tcaactgtag aatattatga gcgtggagaa agtcaagcag tcacaaatgt aaaaatcgat
    67741 aacgtcaacg gtcttatctg tttagatgaa ggcgatagta tgaatgaaat tgctgcagac
    67801 catgaattat cagaaactgc tttaattgat caaatcgaat tagctagaca atactttgtt
    67861 ccatatgcag atacctggtc caagcataag aatgacatca tttttccaga ggaacaatga
    67921 gagcaactga tccagtaatt atccttgagg aagccaaatt tatttggact cacgaagaga
    67981 tagagcaagc acgcttgctc ttttctcaag gagttaagcc gagcaaagta gctgaaataa
    68041 tgggtcaaaa gattcttgat gtcggattgc ttttgctcca tctagcagaa aaaatttgat
    68101 ttgaggtgaa aatgatgatt caacttgcag gcatccaaac agggaaaatt tatttttctg
    68161 gagaaagcaa aagcgaagcc aaccaatggc tattgaaaac ttacacgaac aataagaagc
    68221 taaggaaaaa atatcctgat ttgttgctaa aggatgatca gattatgccg gaaccgatga
    68281 ttttgacccg taaggaggga agttgaaact aaaaaaccag ccgtgtgggc tggagttgct
    68341 atttcttttt aataatgagt ttttctaaat ttaattcatt ctcacgagtt tgcgtccaat
    68401 aatcagaata atattttaat tgtttaagag taggaatgtc gtcttcggta aaattagaaa
    68461 ataattctgg agaattatct ttttttaatt gttctaatcc tgattcttta aaacgttgtt
    68521 caacgagttt tactaaattg ttgatattca tgataaatca cctttttaaa tttaatttcg
    68581 atttgattaa tcgatacaag tattatatca aataaaaaag ctgatttagg gggtcttaca
    68641 aaaacactaa aattttttca aaaagaggag gaacggtggt gaaagtcaga gaacaattca
    68701 agatagtgag cgacgaaggt aatgtttaca aaataaagat cgttaatatt aatgaatatc
    68761 gagaagcaga tcacaaatat ttggttgact tatacatgaa cgataaatat ttgggagaag
    68821 gattttgtgg agattcactt tttgatgatg agcgaatttt gaaggaggaa acagaatgat
    68881 ccacgaacta aaaatactac cagaatattt tgaagcagtg acgagcggac gcaaacaatt
    68941 cgagatccgc aagaatgatc gtgacttcaa agttggtgat cagttaatac tacgtgaata
    69001 taaaaaatac attaaacatg aattacctgg actaacgaga ccaacattta gttacactgg
    69061 tgactcatac aaagcagaga tcacttatat tactgattac atgcagaagt ccggatatgt
    69121 agtgttgggg attaaagggg tgaattaaat gaaagtatac atcttagcta tcacagaggg
    69181 cacatggatg ttcccagtcg gttccggaaa aatctataaa tcaaaaacag ctgcttataa
    69241 agcatttgaa aaatacaaaa aagaaaacgg tggcggcact aacgcaaaga ttctagtggc
    69301 tgataactgg catgaagaag gtgaacgtaa ttgatttacc tgttcatttt cgtggtacta
    69361 actattttta tgctagtaat cttattcaaa aaaagatgag taaagacgaa cgaaaaattc
    69421 tggccaactt ttatttagta ttaaaaatcg aacaaaatgt gttttcggaa aggagaattg
    69481 tatgggaaaa acaaaatcaa agattaagaa gaaaaaacga cgtttggaac aaaaggcaat
    69541 ccagaatgga acggctaaga aaaaataaaa aagctacccc tttcgagata gcagcgcaat
    69601 actattctac cataaggggt ggcgtttgtg agatttcatt ggttaaaaga ttaccaagaa
    69661 ctagatgagc agattcttta cttaaagtgg aatcttaata aaagtaagct tgaattgaat
    69721 cgatgggtct gtggtgattt agcaaacgtc cgcatcgaaa agaattcgag aacttcttct
    69781 ttagaagaga atatccaaaa gatagaaaat gaattggaat tgttgatcga gcaaaaagaa
    69841 gaaatgttat tgctgataga tagcttttct ggtatcgata atcaaattgt taagatgaaa
    69901 tatgttgatc aaatgagctt agaagatatt gctgaatctg ttgggtatag ctcatcgtat
    69961 gtcagacagc gacatgcaga aattagaaag acattgaact ttttagatga gtatgaacac
    70021 agacaagctg accgattgaa aaaagaaaac gaaattgatt tttacaatag cgagaagtac
    70081 aaagaacaac tctctttatt ctaaaattac actgttcact aaatgttcgt aaacattgtt
    70141 agcagcatga tattctatta gtgtcaaaaa aatatgaaag agccaagata tcccaaccgt
    70201 tttattaatt ggtatctgtg gctcttttat tcttttaatt agtagttggc ataaagttat
    70261 ttccatttat aaattgatca gctaaatcag catcataatc agccgcgata aattgcgctg
    70321 ctgatttaat aaatgcatcc atatcatgaa catctttatt agtatgtcta cgaacatagt
    70381 gcgtttcatc attaccaatc caagtagcag ctttggctaa tctttggagt ttaggaaaat
    70441 cattcaaata attttcaatt gttttaccta ataacatttt ttttatttta tcttcatcct
    70501 ctgaattttt agatattgca taatctttaa ttagaaattc tagagctttt ctataaccaa
    70561 ctccagcaat ttgttccaat tgctcagatt tagcttgtgt ggcttgagta taaatatcaa
    70621 caaatgtagg agaaactttt tcaatatttt ctggtaaatc tattttaatc ggcggacggt
    70681 aataatcttt aattaaaata ttttcagaat atccttcatc ttcattaaac gtatttataa
    70741 attgtaaact ataaaatttg ttgcactcat catttataca ctgtactaat acgccaaaga
    70801 ttctatcatt atcgaacgca ctatatgatg aaaccccaaa atacacatac ggagtcatta
    70861 tatttccgca atgcggacaa ctatttggca aataaattgt ctctgtattt tgacgatcac
    70921 gatacttaat attaacttct tgaagcatta aaacacctcg catctttttt ataaatcata
    70981 ccacaaaata tctgatagga gagtaaacaa atgaatgatt ttcatgaagc ggtacttagt
    71041 attgaagtag aatcaagttt ggctaaagtt tataagaaag caattgaagc agagaatagt
    71101 ccgtacagag aaaattggaa tggtaatcat gcacatgtgc aagtggagaa tgatgattac
    71161 agaactggaa tgaatacgtt agttatttct ttattgtcac acactctacc caatctgcag
    71221 gaaacgattg aatggtatga gcgaatgggc gcaaaagtaa tccgtacaaa ttacaaagga
    71281 gagaattaaa atggcaatga tcaaaattag aacatccatt agtggaacag agtattggga
    71341 ttcagaaaag aaaaggacag tagttgttcc gaaagatcaa gaaccaaatt ttgaagtaaa
    71401 agaagaaaaa ggaattttga gtgatggcaa atcatttgtt gctgtcaatg gcgaactcat
    71461 agctcatagc agtgaaattc ttgatagcga tggaaacact gctgctaatt ttgatggaga
    71521 cgaagctact aatgatcagt ccactgaaga tacggatgaa ctggacaata tgactgcaaa
    71581 agaattgcgt gcatatgcta agaaacatgg tattgatatt cctggtgcta tccgtgcaaa
    71641 aggcgatatc ataaatctta ttcgtgaagt agaatgaaat actgtcagtt tgacggatgc
    71701 acgaacaaaa tagcaaaggg tatctactgt actgaacaca agagatcaag cagatcacgc
    71761 aagaagaagc agcaagcaaa gtctgtttat catcatgaga acaaaccatt ctatcgaacg
    71821 caagcatgga aagatatgcg tcaatttatt tatgaaagag aaggtggtca ttgtcagcgg
    71881 tgtggtcagt tcatatttgg aaagagagca cacgtccatc acattgtacc aatcaaagac
    71941 aacgaactgc ttaagcttga tccaaacaat ctcatgctat tatgttcaaa atgtcatcca
    72001 attgttgaaa acgaaacgga agacaaaaaa gtttttcctt cgtatttcaa ttgaagcccc
    72061 cctattcatt ttcaaaattt tttcgcgtgg ggagataggg tagcggggag tcacgcgcat
    72121 cgttaggtca aatttttcaa aaaacaaagg ggggtgtata caaaaatgac gactaaagcg
    72181 caacgtaaag cgattattga tgaaaaagta agtgctgaaa aagctcgtat cttagaaata
    72241 atgaatttgt ctgatttgta caccatcact cttgatccat taatcgaatc atacttggat
    72301 atttttgaaa tttaccaaca caaatattta ttgtggaagg aaaaaggatt tccggagacg
    72361 caaaaattca cgaataaatc aggagctact aatcaatcaa aacatccatt ggcacaacaa
    72421 gtagaaactt gggcagataa gaagatgaaa gctctagatt tattaggatt aacaaacaaa
    72481 gcaaaaactg gtagactaat tgctggagga tcgaccgcaa gaaaagatga agaaattaca
    72541 cgcccagaag taaagccagt agatgaacta gcagcgcaca gaaataaatg gcgtaagaag
    72601 gcaggtgctg aaaaatgatt gaacctggtg taaattatgc tgatttattt gcaaaagaag
    72661 taagaaagaa acctaagaag tatcctaaga ccgttcgttt agcaatagat cgctggtatc
    72721 agtggaaaaa aagaaaagat atttggtttg atgtagatcg cgcgaatgaa atgatggatt
    72781 gggtagaatc atttattgtt cacacaaaag gagaaatggt gggaaaaccg tttcttttag
    72841 aaccctggga aaaatttatt tattcttgga tttatggttg ggttaaagaa aatgaaaaag
    72901 gccaaattgt acgcgttact cgtgaggctt atgtccagat tccaaaaaag aatggtaaaa
    72961 cattgattgc tgtcggttca ttaggatatg caatgtacgg agaaggggcg ttatcagttg
    73021 attgctatgc ttgcgcatct gattttgctc aagcgcaata tgctgccaaa ccttttgcag
    73081 ttacaatctt aaacaatcca atcctacttg aaggaactaa aatatttagg gggccaaagg
    73141 gaactgtttc tagtattact tatgactata ttcatgaaga tatggcttac tcaaataaat
    73201 ttattgttca aaccaagaat attgataata tcgagggatc caatccatat tttgtattga
    73261 acgatgaatt gcataaacag gagaaaatgg aacagtatga caattttaag tctgctcaga
    73321 tatctttacc acagccattg atgtttaata tctcaactgc tgggaaaggt tcgtcgtctg
    73381 ttggaatgcg tgtatatcgc gaagcaaaag aagtgttgaa acgtgatgat aatgattcaa
    73441 actttgttct aatctatgag ccaaataaaa attatgattg gacggataga aaagtctggg
    73501 aaatgtgcaa tcctaactgg ggaatatcag tcgatttatc tgctttagaa tcggcattta
    73561 aaacagcgca acggtcagct cattccaaag ctgaatttct aacgaaacac ttggatgtat
    73621 ttgtgaacgg cgcggataat ttctttgaac aggatcaagt agagccatgt ttagttacca
    73681 cacaagaact tggtgattta agtggcgaac catgttatat cggtttagat ttatcacgta
    73741 cacgagattt gacctgtgta tctttaaact tcccaacatg ggatgaagat ggtaaagcgg
    73801 ttcttaaagt aaagcaactt tattttattc cgaatgaaga tttagagttt cgagaaaaag
    73861 aagataatgt tccttacagt gatttggctg aacaaggttt cgttgaattt tgcgacggaa
    73921 aaatgattga tcaagatcag attttgcagt acatagagga ctgcatgaat ttatatgata
    73981 ttcagcaagt gaattatgat ccagcgatga gtgacaaact tgttgagaaa ttagaaaact
    74041 taggattgga atgcgttgaa gttgctcaat atcctaaagt attgaatgct ccttttgacg
    74101 atgtcgaacg gttgttttat gagaaacgaa ttcaatttga taatccatta ttcctttatt
    74161 gcactttgaa cgttgtagca attactaaca tcaatggaca aaaagcgcca agtaaacgtc
    74221 aatcaaagaa aaagattgat gggttcgtgg cgtttttgtg tggtcataag gaaacgatga
    74281 atcaaatgac agatattgat tcagatgagt tagatgatta cctaagttcc atctatagat
    74341 aaatagaaag gcggtgaaaa aaattgaaat tacgtgatcg gttatcgaat gcagtctatt
    74401 cttttatgga aaaacgtgga tatatcgagg atatttttgg acactacaca cgttatggtc
    74461 aaagatatgt gacggattca tctatcatgg aatcttctga tgtttatgaa ttagttcaag
    74521 acatatctaa tcaggtggca ttggccacac caattgttat tggtcccgac ggcaacgaag
    74581 tcaaagatca tcacttacta aatattttga agagtcccaa tgattatttg acaggattcg
    74641 aatttaccaa attggaaaca aatactttat tgatcaatgg tgagacattc ccattaacgg
    74701 atagggatca gcttcatttg gcgtacggtg taacaactaa aatcaatgaa cgacttcaag
    74761 aagaatttga aatgaatggt caaaaaatac ctggtcaaat gattcgacac attaagaaca
    74821 tcggaactga ttcattaaaa ggtgctggaa taattgatct tgcaagaaac actctagaag
    74881 gcgttctgag cgctgaaaaa gttttgacgg acaaatatac taaaggcggt ttacttgcgt
    74941 tcatgcttaa actagacgcc cacatcaatc caaataatag cgcccaaaca aaaattgtca
    75001 aagctatatt ggatcaactg gaaggaacgc aaaatgagag tgatcattct gttaagatga
    75061 ttcctttggg aaaaggatat tccatcgaga cattaaaaag tcctgttgat gacgcggcaa
    75121 ttttgaatta tttaggtgtt tacaaaaaag acttaggaaa atttctagga atcaatgttg
    75181 atacgtatca atcgctgatg aagacagata ttgaaaaagc gatgatgtat ctgcacaaca
    75241 aagcaatcaa accaatattg aaaaacaaga gcgaacatta caccgctctt ttttttatgc
    75301 ctaattctgg ctatagagtg gaatggaaaa ttaatatttt ggattttgtt ccttactcaa
    75361 caaaaacaaa tattggctac aacatcgttc gtaccgggat tacaagtcca gataacgtgg
    75421 cagaaatgct tggttttcct aaacagaata ctccagaaac acaagctatc tatatttcaa
    75481 atgacttatc taggattggc cagaaaaatg caacagatga ttccttacca acgaatgatc
    75541 aagacttgaa aggaggtgat ggaaatgaag aagaaggaaa ttcgcacgat tgacatcacc
    75601 aatctttcaa cacgttccga tgaagaaact catacgagga ccattagcgg ctacgctgct
    75661 gtattcaaca gtcaaacact tttatgggat gacttaagcg aagtgattgt tcctggtgca
    75721 ttctcaaagg caattagtaa ttcggatgtt cgttgtttgt tcaatcacga ttggtccaat
    75781 gtgctaggac gtactaaaag cggaaccctt cgccttgaag aagatgaacg tggtttgaaa
    75841 ttcgaagttg atttaccgga cacaacggta gcaagggact tagttaaatc tatggaacga
    75901 ggagacatca accaatgtag ttttggtttt gtgccgactg aagaaacgtg ggactacaat
    75961 tctgaaccaa tgcttcgaac aatccatgaa gtggaattat acgaggtttc tattgtgcct
    76021 ttgccagcat acgaagatac agaagctgca ttaagaagtc gtgatgaatt agaaaaaaac
    76081 gtcgaagaaa gaaaaaaatt aatcaaaaaa attaatcaag cgctagaagc gtaggaggaa
    76141 aaatacatgg ataaagaatt attgaaaaaa atgaaggcac gtcgtgagca acgattgact
    76201 gaattacgtg aaaaagttga atcaggagaa ttacgcgaag cagatttaga agccgtgaaa
    76261 gaagaaattg acagtgttat tgatgaattg aacggaatta aagacgaatt aggcgcagat
    76321 tctggaactg atgaaacaga cgacaataca gatgatcaat cgaatagtac ggattcagac
    76381 gaaagccgtt ctggtgaaga caacgatcaa gaagaagatt cagacagtga agatagttca
    76441 gaaaatcgtt ctggaatgat tactcaacag caacgagatg gattacttgg atcaattaag
    76501 aacggattgg aggcacgtgc aaaaatgacc aagaaacaaa aagatcaaca actacgaaaa
    76561 gcatttgcta attttgtagc tggaaatatt tctgaagcag aagctcgagc tttagggatt
    76621 gaagctggca acggttcagt tactgtccca gaagtaattg catctgaagt tattacttat
    76681 gctcaagaag aaaatttact tcgtaaatac ggaacagtgg tgcgaacatc aggagatgtc
    76741 aaatatccaa ttcttgtgaa gaaagcagat gcgaatgtaa acaagaaaga gcgttcaact
    76801 gatattgctg aaacagctat tcagtttgat gaaattttgc ttaatcctgc cgaattcgat
    76861 gctttggcaa cagtaactaa aaaattatta aaaatgtctg gtgttccagt tgaagatatt
    76921 gttgtggaag aattgaaaaa agcttatgtg cgtaaagaaa tcaattatat gttcaatggt
    76981 gatgacgctg gaaatgaaaa tcctggtgca ttagccaaaa aggctgtagc atttaaaaaa
    77041 cctttagatc taactgcttc aggtgctggg caaaaattat atgatgcatt aatcgaattt
    77101 aaaaatacac cagtgacaga agtgatgaaa aagggacgct ttattattaa tcgagctgct
    77161 ttgactgcca ttgaaaaaat gaaaacagat gatggatttc ctttgttgcg gccattcaca
    77221 caagcagaag gtggaattgg ttaccaatta gttggctatc ctgtggattg gacagatgca
    77281 gcagataaaa agggtgaacc agacacgcca gttttatatt ttggcgattt ttctgcattt
    77341 aaaattcaag aagttattgg tgccttggaa attcaaaaac ttgttgaaaa attctctggt
    77401 aaaaatcaaa ttggattcca aatttacaac ttgctagatg gtcaattggt ttattctcca
    77461 tttgaaccgg cagtatatcg ctacgaaatt acaaaaccag ttggtggtta agatggaaga
    77521 gcaaactaaa gaattgtctt tagaggaaaa attcaaatca catattcatt ttgaagaggg
    77581 catggatgat tctttgctct ctttttattt aaatatggca aaagattatg tcaaaacagc
    77641 aactggtggc caacaagaat atcttatttt gatggttgcc ggcattgcct atgaatatag
    77701 agtttcagag gatgaactcg acaaagctat gaatgccatg acgccattta tcgtgcaagg
    77761 agcgattcaa aatgccgaag agacagacta ataatctcag atggaaagcc gaattgctag
    77821 acatcaaaac aggaacagac ggaaacgatc gtccaactac aatttacgaa tttaagcgtc
    77881 taatattcta tgaagaactc ggtgtgactt ctcaagaaaa atatttatca cagcaagcca
    77941 agacagacgt tgtcagacga attaaagtca gatgggataa atccatcaca gagaaattaa
    78001 gtgcgctcaa aattgattct gtaacgtata acattactcg catttatacg aatcccgatg
    78061 caagagaaat ggagttgagt ttagcttatg tcgattagct ttgatgaatt gaaaacagcg
    78121 ctgaaatcaa caaagttagc agtgttcaga gacaaagcca gattagggac gatgtatcca
    78181 tacattgtgt actcaaatgt gagtaacagc aaaaaaatgg catccggtaa agtctataaa
    78241 aaattacctt attaccaagt ctcttttttt acacttggaa cagaacagga tttagttgtt
    78301 attgaacaag cgctgcaaaa tgctggcatt ccatattcag attttacagg tatacaaggt
    78361 gatgagaacg acgataccgt aaccaactat tacacatatg tgaggtgtgt ggaaaatgcc
    78421 aagtaataag aatggttttt ctgaaatatc ggattattta ggaaaccttt caaaagttga
    78481 tccgaaaaaa ttatcgttgg aatctttaga agaagcagct aacttttatc taaagcagtt
    78541 gcttcctaat attcctaagt cgctcttaaa aaagaaacat atgcgcgatc aaataaaagt
    78601 agtagttgaa aacgatcaag tgaaagttca atttgaagag acggcctttt actggcgctt
    78661 tgccgaaaat ggcacaacaa atcaacgtgc ccaacatttt gcaagtggta cgtatgaaca
    78721 aaacaaagaa aaaattgaag aaattatgac tagaaaaata cttgatttat gggaagggtg
    78781 attttaattg ggaaaacaag atacttttta ttttgaagga ttggacgaca ttttaattgc
    78841 gatgatggca acgccagatt ctgttggaac tgcaccgact tatagtgaag ttttacgatt
    78901 gccaattgcc acaaaaatcg gtgtcaaagg gaatggaaca gcattagaaa aatgggcatc
    78961 cagcaaaatg tttcgtcgtg tgtcgcgaga aacaaaacat gaacttgcgt tggatcacgt
    79021 gggtattccg atagaagtga tggatgaaat caaaggatta gttgctaaaa gcggagtaac
    79081 gtttagcaaa aataccgcac gagaatttcc gtacttcgcc tttggattta ttggaaatat
    79141 tgaaaatggc ggtaaaaaag cagtttggta tcctaataca caactatcca atgtgattga
    79201 cgaagagtat gccactgcag aggaagaaac aaaaattgac gatgtaactg caaatctggt
    79261 ttccatcgga ctgaaataca ataatgtgat gtatgcaagt tttgattcaa atagagacgg
    79321 agcttctcta gaattattta ataaattcat tgctcaacct gtatacgatg aagaacagtg
    79381 gaaaacattg gctaaagttg gaggtgcggg ttaatggctc ggttatctga ttatggaatt
    79441 cacgttgaag acttaaaaaa ttctgctgtt gtaattattc aaggcgcaga attccctatt
    79501 tcatttacta tgcaaacgat ggaatttata gcagatgtgt atggtggaga ttattcgcaa
    79561 tttgaatctg atatgaacgc catgctatct aaaaaagaag gacaaatttc ttctgctaac
    79621 ttatcgccta gtgatctaaa aatcatgcgt gccttgattt atgccatgct gcgtactggt
    79681 ggtttagagg aagatccaga aactattttc aaatttttgg gaatgagtgg agaggtgttg
    79741 tctgcttata gtacctgtat ggaaattttt gctagccaga catttcaggt ggaagaccta
    79801 aaaaaatcca agaagccaca agactttcaa aaagcgcaag caaaaagaaa gaaaaacaaa
    79861 aagaatcgga agagatagga actccttgga gtttttatat ttacgttgct ctcactctat
    79921 tgaattggag tgagagtttc tttttaaagt ctacacctaa cttgtggctc aaatcttatt
    79981 tacagtggtt acaacaaaac accgattttg aaccacctca atctgtaact atggataaat
    80041 cgccttggtg gtagaaagga gcgctaagat aaatggctgg taaagaatct gatgtcgttc
    80101 ttaattttaa aacgaatggc gaagtcagtt attcgaaaac aatcaaagaa atcaacaaag
    80161 aaatgaactt agccgctgcc gagtacaaaa accaagtgtc tgcgatggac aaggatgcaa
    80221 ctcaaacaga aaaattgcga gcggctaaac aaaaattaga aaaacaatta ggattggctg
    80281 aacaacgatc tcaaatgctg agagaggaat atgaaaaatc tgtcaaagaa accggtgaat
    80341 attctgctga atcagaaaaa ctttataaac aattactcaa ttctgaaacc ggagaaaata
    80401 aacttcgtac agcattagaa cagacgaacg atgcacttaa agaacaaggt gacgtttctg
    80461 ttgatacagc aaaaaaactc cagaaaatcg aagaaacagg tgagaaagta aaaggcgttg
    80521 gtgaaaaaat gtctgttgga gtaaccgcgc ctattgtagc ggcaggagca gcaggacttg
    80581 cagcatttgg tgaagttgac gaggcacttg ataccatcat tacaaaaacc ggagcaacag
    80641 gtgatcaagc tgatagactt tcacagtctt tcaaaaacgt tggttcaaat actcatttac
    80701 ctttacaaac ggttggggaa gctattggtg aggtaaatac acaatttgga ttcatggata
    80761 aaaaactgga agattcaacc aattatctcc tacagtacgc tgaaatcaat gatacagatg
    80821 tttcgcaatc agcaatatct gctcgacaag ctattgatgc ttatggacta gaatatgatg
    80881 atttgaattc tgtccttgat gtaacaacga aaacatcgca gaatactggt caatctgtag
    80941 acgacttgat gcaaaaagca attgatggcg caccacaaat taaacaatta gggttgagct
    81001 ttggtgaagg ggtcacttta cttggacaat ttgagcaatc tggtgttgat tcaagcgcag
    81061 ctttaagtag tttatctaaa gcaacagtgg cttatgcgaa agacggaaaa tctttaagtc
    81121 aaggactaga agaactacaa gacaaggtaa aaaatgcagg ttctgaaaca gaagccatca
    81181 atgcagcagc agaagtattc gggactaaag gcggtcctag aatggccgat gcaattcgta
    81241 gaggtacttt gaacctggaa gatttagcaa aaactgctgg agaaagtggg ggagctgtag
    81301 gagacacatt cgatgctact ctcgacccaa ttgatcaagc cgatcaagct atgaacaatg
    81361 caaaattagc aatggctgac gtaggtgaag cagtccaagt aagccttttg cctttttttg
    81421 aaaaagcaac ttccttatta caggaattct ctaaatggtg ggggtctcta gatcaagata
    81481 caaagaattg gatcataaca attgctggta tagcggcagc agttggacca gtactaatcg
    81541 ttcttggttc acttatggga tctgttacaa aaatcgttgg ggaagttaaa agttttatag
    81601 gagtttggca aggacttgct ggtttatttg gaatgtcggg aggctggttt gcattagcag
    81661 ttatagctat tggcgcatta gttactggac taatttgggc atataataac gttaaatggt
    81721 ttcatgatgg agtgaattct ttcttcaaag gtgtttcaga tgtagctgtt gaaatattta
    81781 attttttaag tggttatttt ggtggatttt tcgatggtgt tatagcaaat ttcaataatt
    81841 tttataatgc tggaaaacga attttcagtg gttttctaga ttttatcact ggagtattta
    81901 cagggaattg gtcaaaagca tggcaaggtc tagttgatat ttttggaggt attttcgatg
    81961 gtattgttgc tgtagggaaa gctcctatta atgccatgat tggattgatc aatggattta
    82021 ttggtggatt gaataatata aagataccaa aatgggttcc tggtgtaggt ggaaaatcat
    82081 tttctatttc aaaactgcct tatttagcac aaggcggtca tctaatcaat ggacaagcga
    82141 ttgttggtga agctggacct gagttactga ctgccaagaa tggtaaaacc acagttactc
    82201 cattatcaga cgaagaaaaa cgcaaaggga tcagtggaag agtttctgga ggtagcattg
    82261 aacagcatat acacattggc aaagtcgatg caaacaatcc atctgagtta gatcgtatga
    82321 atcgtaaatt tgctaaagca aacaaacagg ctattcgtga tttgggaggt gttcctattt
    82381 gagtcgacaa tttatgaatc cagatgaacc aaattttatt tggaaaaatc ggaatgcagt
    82441 aattgatatg aattgcatca ttgaggcaga gcttccagat attatgccga acaagcgata
    82501 cgaaacttac acgattcagg gccgaagcgg agaattgacg gagacgtttg atgactatga
    82561 accttttgat ttggaaatag aagggatcac tattccacat tcgaaactga gagaagtcaa
    82621 acgatggctc actggtaaaa gccaattaat cacacataat gatccagaca agtatctaga
    82681 ggctatctgc agcatggata aagaagtccc tttcgaaaac gagtggggct tcttttatac
    82741 attcgatgtt acttttcgtt gtcagccact caaaagaaaa atcggtgaag cacctaaaat
    82801 ttttcattct agtgttatgg atatttttga tcatggtgac gaaattgcac atccctatct
    82861 ggaaatagaa tcgaacggag gagatataac gatcgctgtc ggtgataaaa gcctaacaat
    82921 acttaatacg ttagctgcaa caatcgtggt tgatactcag cttggaaaag caatacaaga
    82981 aggtttgaat ttatttacgc gtggtgattg gccagtattg cagcctgaat ggaatcaggt
    83041 aaaaatatcc ggaaaattta aagaagtgcg gttttggaat aggagtgtgt atctgtgacg
    83101 caagaattta tttatgctta taaaaaaatg ccagatgatt tgagcgtgaa cggagcgtcg
    83161 ttagttgatt gggaagattt gccagaaatc aatcgtgtgc tgaatggcca atatcgtttt
    83221 tatggtaact attcaagaag cggacagtat cgctcttatt taaaaaaagg aaattttata
    83281 aaagcgaagg taccagatgg atcatggcaa tattttgaaa tttataatgt caaaaagaat
    83341 ttgacctccg tttctgtaac agctcggcat attggtttta tggccaataa gaactttatt
    83401 gtcaagtcgt ttacggataa cggcaatggg tctcagatca tgaccaatct taaaaacagt
    83461 ttggcatttg atcaaaaatt taactatctt tcgaatgtag ggacaacgca ccaatttaca
    83521 gcaagacaag tcgcgccagt tgaagcaatc attggttcaa ataacggtaa tcaaaatttg
    83581 actggcgtga ctagcgcgga acttgatatg gataactatg atttgaaact ggtaaaacag
    83641 attggatcag ataatggttt tcggattgat tttggcatca atttagaagc aatcgaagaa
    83701 gaaattgatg aagaatcaat tgtcaatagt ttgtatttgg ttggcggtgt tcctgataac
    83761 gattatgatg aagacaaaga acctatcgaa tatggttatt tagaaattga tggagtaacc
    83821 aacgagaatc gacgtattgc caaacgtgaa aacggagatt gtaaaacagt tgatgaattg
    83881 atcaaatggg gcaaaacctt atttgacaat gatcgtatcc atgagccaaa agctactcat
    83941 acagtcagta tggtggcgtt ggaacacaca ctcgaatacg gtgacatgta tcaagagcta
    84001 gcgtctttac actttggtga tgtcgctcat gttagggcga aagagctaga tatcgagata
    84061 aaagagcgta tggtcgaata cacttatttc ccaacgcttg ggaaatataa ggatattgtt
    84121 ttaggtaatg atttatcact gtacacttcg gcagtaaata ctcaagcaca agaattgaaa
    84181 aagaaaatcg acaatcgaac agaaacgtta gttcaaaatg tgttgaatgc gacggcatgg
    84241 atcactggta attctggtgg gcatgtcgtt tttcgtccag aaaaagctcc atcagagatt
    84301 cttatcatgg atactccaaa tgtggcgaat gcaaagcgag tgtggcgttg gaatttaaat
    84361 ggcttaggtt attcagacaa cggagtaaat ggcccgttcg gtattgctat cacatcaaaa
    84421 ggagaaattg ttgctgactt catcaaagta ggaacaataa atgcagaagt attcgaaact
    84481 tcctttaatg cttatggtga tgtgttaaaa cttgtaaaag gcacgcttca aatttggaat
    84541 gaaaacaaaa aaatcatgga gctaaccaaa aaaggtatgg aattctggaa tagaaaagaa
    84601 tccattggaa cgatcggaac aacagattca gcaggaaatc catttccaaa tgctgtaaca
    84661 cctacacctc ttgaggaaaa ttctttggta atacgtacaa atggcgatgg aaaatacatt
    84721 ttaatttctc caactgcaga aaaaggattt gtgttattag gaaacggaaa agcatattat
    84781 ttcggtgatt tagatattca gggaaaactt actgttagag gtaaagaagt gataccagga
    84841 caaaatggcg gtccatctgg cggaggagaa acacctggtg ggtatcctga tgaattaaaa
    84901 acagatgcag aaaagagagc ttggagaat
//
LOCUS       DAZAJZ010000003        76223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000003 DAZAJZ010000000
VERSION     DAZAJZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.003"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..251)
                     /locus_tag="ACG86D_000200"
     CDS             complement(<1..251)
                     /locus_tag="ACG86D_000200"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN3139382.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEK"
     gene            complement(215..340)
                     /locus_tag="ACG86D_000201"
     CDS             complement(215..340)
                     /locus_tag="ACG86D_000201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139383.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(543..1022)
                     /gene="rlmH"
                     /locus_tag="ACG86D_000202"
     CDS             complement(543..1022)
                     /gene="rlmH"
                     /locus_tag="ACG86D_000202"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN3139384.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1450..2739
                     /locus_tag="ACG86D_000203"
     CDS             1450..2739
                     /locus_tag="ACG86D_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN3139385.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2778..3383)
                     /gene="ytpR"
                     /locus_tag="ACG86D_000204"
     CDS             complement(2778..3383)
                     /gene="ytpR"
                     /locus_tag="ACG86D_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN3139386.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3588..4052)
                     /locus_tag="ACG86D_000205"
     CDS             complement(3588..4052)
                     /locus_tag="ACG86D_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3139387.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4074..4394)
                     /locus_tag="ACG86D_000206"
     CDS             complement(4074..4394)
                     /locus_tag="ACG86D_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3139388.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4538..5617)
                     /gene="pepA"
                     /locus_tag="ACG86D_000207"
     CDS             complement(4538..5617)
                     /gene="pepA"
                     /locus_tag="ACG86D_000207"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN3139389.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5817..6167
                     /locus_tag="ACG86D_000208"
     CDS             5817..6167
                     /locus_tag="ACG86D_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN3139390.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6390..7337
                     /locus_tag="ACG86D_000209"
     CDS             6390..7337
                     /locus_tag="ACG86D_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3139391.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7394..8812)
                     /gene="pepV"
                     /locus_tag="ACG86D_000210"
     CDS             complement(7394..8812)
                     /gene="pepV"
                     /locus_tag="ACG86D_000210"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN3139392.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8961..9806
                     /locus_tag="ACG86D_000211"
     CDS             8961..9806
                     /locus_tag="ACG86D_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN3139393.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9810..10250
                     /locus_tag="ACG86D_000212"
     CDS             9810..10250
                     /locus_tag="ACG86D_000212"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN3139394.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10300..10494
                     /locus_tag="ACG86D_000213"
     CDS             10300..10494
                     /locus_tag="ACG86D_000213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139395.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10630..11307
                     /locus_tag="ACG86D_000214"
     CDS             10630..11307
                     /locus_tag="ACG86D_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3139396.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(11715..12404)
                     /locus_tag="ACG86D_000215"
     CDS             complement(11715..12404)
                     /locus_tag="ACG86D_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139397.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12379..14508)
                     /locus_tag="ACG86D_000216"
     CDS             complement(12379..14508)
                     /locus_tag="ACG86D_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN3139398.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14556..15893)
                     /locus_tag="ACG86D_000217"
     CDS             complement(14556..15893)
                     /locus_tag="ACG86D_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN3139399.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16145..16504)
                     /gene="rplT"
                     /locus_tag="ACG86D_000218"
     CDS             complement(16145..16504)
                     /gene="rplT"
                     /locus_tag="ACG86D_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN3139400.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16557..16757)
                     /gene="rpmI"
                     /locus_tag="ACG86D_000219"
     CDS             complement(16557..16757)
                     /gene="rpmI"
                     /locus_tag="ACG86D_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN3139401.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16829..17353)
                     /gene="infC"
                     /locus_tag="ACG86D_000220"
     CDS             complement(16829..17353)
                     /gene="infC"
                     /locus_tag="ACG86D_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN3139402.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17397..17522)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17673..18305
                     /locus_tag="ACG86D_000221"
     CDS             17673..18305
                     /locus_tag="ACG86D_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN3139403.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18307..19212)
                     /locus_tag="ACG86D_000222"
     CDS             complement(18307..19212)
                     /locus_tag="ACG86D_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3139404.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19297..19956)
                     /locus_tag="ACG86D_000223"
     CDS             complement(19297..19956)
                     /locus_tag="ACG86D_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3139405.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            20272..21216
                     /gene="mvk"
                     /locus_tag="ACG86D_000224"
     CDS             20272..21216
                     /gene="mvk"
                     /locus_tag="ACG86D_000224"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN3139406.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21209..22186
                     /gene="mvaD"
                     /locus_tag="ACG86D_000225"
     CDS             21209..22186
                     /gene="mvaD"
                     /locus_tag="ACG86D_000225"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN3139407.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22199..23284
                     /locus_tag="ACG86D_000226"
     CDS             22199..23284
                     /locus_tag="ACG86D_000226"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN3139408.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23281..24345
                     /gene="fni"
                     /locus_tag="ACG86D_000227"
     CDS             23281..24345
                     /gene="fni"
                     /locus_tag="ACG86D_000227"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN3139409.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24594..25397)
                     /locus_tag="ACG86D_000228"
     CDS             complement(24594..25397)
                     /locus_tag="ACG86D_000228"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN3139410.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25417..26259)
                     /gene="kduI"
                     /locus_tag="ACG86D_000229"
     CDS             complement(25417..26259)
                     /gene="kduI"
                     /locus_tag="ACG86D_000229"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN3139411.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26275..27282)
                     /locus_tag="ACG86D_000230"
     CDS             complement(26275..27282)
                     /locus_tag="ACG86D_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN3139412.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27289..27933)
                     /locus_tag="ACG86D_000231"
     CDS             complement(27289..27933)
                     /locus_tag="ACG86D_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN3139413.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(28033..28791)
                     /locus_tag="ACG86D_000232"
     CDS             complement(28033..28791)
                     /locus_tag="ACG86D_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN3139414.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28918..30069)
                     /gene="fucO"
                     /locus_tag="ACG86D_000233"
     CDS             complement(28918..30069)
                     /gene="fucO"
                     /locus_tag="ACG86D_000233"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN3139415.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30151..30462)
                     /gene="rhaM"
                     /locus_tag="ACG86D_000234"
     CDS             complement(30151..30462)
                     /gene="rhaM"
                     /locus_tag="ACG86D_000234"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN3139416.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30464..31309)
                     /gene="rhaD"
                     /locus_tag="ACG86D_000235"
     CDS             complement(30464..31309)
                     /gene="rhaD"
                     /locus_tag="ACG86D_000235"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN3139417.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31323..32579)
                     /gene="rhaA"
                     /locus_tag="ACG86D_000236"
     CDS             complement(31323..32579)
                     /gene="rhaA"
                     /locus_tag="ACG86D_000236"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN3139418.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32576..34036)
                     /gene="rhaB"
                     /locus_tag="ACG86D_000237"
     CDS             complement(32576..34036)
                     /gene="rhaB"
                     /locus_tag="ACG86D_000237"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN3139419.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34189..35058
                     /locus_tag="ACG86D_000238"
     CDS             34189..35058
                     /locus_tag="ACG86D_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN3139420.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35184..36083)
                     /locus_tag="ACG86D_000239"
     CDS             complement(35184..36083)
                     /locus_tag="ACG86D_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139421.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36248..37072)
                     /locus_tag="ACG86D_000240"
     CDS             complement(36248..37072)
                     /locus_tag="ACG86D_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3139422.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(37083..38378)
                     /locus_tag="ACG86D_000241"
     CDS             complement(37083..38378)
                     /locus_tag="ACG86D_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN3139423.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38394..38873)
                     /locus_tag="ACG86D_000242"
     CDS             complement(38394..38873)
                     /locus_tag="ACG86D_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN3139424.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38879..39907)
                     /locus_tag="ACG86D_000243"
     CDS             complement(38879..39907)
                     /locus_tag="ACG86D_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN3139425.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39924..41030)
                     /locus_tag="ACG86D_000244"
     CDS             complement(39924..41030)
                     /locus_tag="ACG86D_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3139426.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41259..43856)
                     /gene="adhE"
                     /locus_tag="ACG86D_000245"
                     /gene_synonym="adhC"
     CDS             complement(41259..43856)
                     /gene="adhE"
                     /locus_tag="ACG86D_000245"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN3139427.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44161..44541)
                     /gene="yajC"
                     /locus_tag="ACG86D_000246"
     CDS             complement(44161..44541)
                     /gene="yajC"
                     /locus_tag="ACG86D_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN3139428.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44638..45783)
                     /gene="tgt"
                     /locus_tag="ACG86D_000247"
     CDS             complement(44638..45783)
                     /gene="tgt"
                     /locus_tag="ACG86D_000247"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN3139429.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            46001..46699
                     /locus_tag="ACG86D_000248"
     CDS             46001..46699
                     /locus_tag="ACG86D_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN3139430.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46867..49506)
                     /locus_tag="ACG86D_000249"
     CDS             complement(46867..49506)
                     /locus_tag="ACG86D_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3139431.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(50125..51156)
                     /gene="queA"
                     /locus_tag="ACG86D_000250"
     CDS             complement(50125..51156)
                     /gene="queA"
                     /locus_tag="ACG86D_000250"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN3139432.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            51349..52524
                     /locus_tag="ACG86D_000251"
     CDS             51349..52524
                     /locus_tag="ACG86D_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3139433.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52528..53169
                     /locus_tag="ACG86D_000252"
     CDS             52528..53169
                     /locus_tag="ACG86D_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139434.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            53166..54083
                     /locus_tag="ACG86D_000253"
     CDS             53166..54083
                     /locus_tag="ACG86D_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3139435.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            54080..54751
                     /locus_tag="ACG86D_000254"
     CDS             54080..54751
                     /locus_tag="ACG86D_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139436.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(55292..55780)
                     /locus_tag="ACG86D_000255"
     CDS             complement(55292..55780)
                     /locus_tag="ACG86D_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN3139437.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(55981..56247)
                     /locus_tag="ACG86D_000256"
     CDS             complement(55981..56247)
                     /locus_tag="ACG86D_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3139438.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(56247..56510)
                     /locus_tag="ACG86D_000257"
     CDS             complement(56247..56510)
                     /locus_tag="ACG86D_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3139439.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            57031..57171
                     /locus_tag="ACG86D_000258"
                     /pseudo
     CDS             57031..57171
                     /locus_tag="ACG86D_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(57313..59136)
                     /locus_tag="ACG86D_000259"
     CDS             complement(57313..59136)
                     /locus_tag="ACG86D_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3139440.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59734..62439)
                     /locus_tag="ACG86D_000260"
     CDS             complement(59734..62439)
                     /locus_tag="ACG86D_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN3139441.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62745..63692)
                     /locus_tag="ACG86D_000261"
     CDS             complement(62745..63692)
                     /locus_tag="ACG86D_000261"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN3139442.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            63882..64151
                     /gene="rpsN"
                     /locus_tag="ACG86D_000262"
     CDS             63882..64151
                     /gene="rpsN"
                     /locus_tag="ACG86D_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN3139443.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(64327..64689)
                     /locus_tag="ACG86D_000263"
     CDS             complement(64327..64689)
                     /locus_tag="ACG86D_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3139444.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64700..65821)
                     /gene="alr"
                     /locus_tag="ACG86D_000264"
     CDS             complement(64700..65821)
                     /gene="alr"
                     /locus_tag="ACG86D_000264"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN3139445.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65837..66187)
                     /gene="acpS"
                     /locus_tag="ACG86D_000265"
     CDS             complement(65837..66187)
                     /gene="acpS"
                     /locus_tag="ACG86D_000265"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN3139446.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(66317..67828)
                     /locus_tag="ACG86D_000266"
     CDS             complement(66317..67828)
                     /locus_tag="ACG86D_000266"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3139447.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(68150..69502)
                     /gene="murF"
                     /locus_tag="ACG86D_000267"
     CDS             complement(68150..69502)
                     /gene="murF"
                     /locus_tag="ACG86D_000267"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN3139448.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69655..70731)
                     /locus_tag="ACG86D_000268"
     CDS             complement(69655..70731)
                     /locus_tag="ACG86D_000268"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN3139449.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            70927..72672
                     /locus_tag="ACG86D_000269"
     CDS             70927..72672
                     /locus_tag="ACG86D_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN3139450.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(72952..73389)
                     /locus_tag="ACG86D_000270"
     CDS             complement(72952..73389)
                     /locus_tag="ACG86D_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN3139451.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(73373..74746)
                     /locus_tag="ACG86D_000271"
     CDS             complement(73373..74746)
                     /locus_tag="ACG86D_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139452.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(75068..75556)
                     /locus_tag="ACG86D_000272"
     CDS             complement(75068..75556)
                     /locus_tag="ACG86D_000272"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139453.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75578..76180)
                     /locus_tag="ACG86D_000273"
     CDS             complement(75578..76180)
                     /locus_tag="ACG86D_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3139454.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21866 a        16281 c        13932 g        24144 t
ORIGIN      
        1 tttttttccg ataccaataa aatatcaata ttcttatttt ctctgtaaac aattaaatct
       61 tcatagctat taagtaaagc atcaaaataa ttgatagcag ctttggtatc atcattagaa
      121 tctatatacc ttttcaaaaa cattttaaaa aactcatcag ataaattgtg ggtttcattt
      181 ggagcaaaca aaaaagctaa aaaattacta tgtcttattt catttttaga tagcttaagt
      241 atctgaaata tatttgtttt cccagtccat ttagatagtt ttcttcaata tcatccatat
      301 cctctaatag attttttaaa cacttttttt catctatcat gttttttctc cttgccatta
      361 ttctaactaa aattttacgt atttgtttct atgtctacat tatcttttta acagtatcta
      421 tttttctttt gcaacttact tggctaccaa agcaaataaa aaagttattt ataaatttct
      481 ccctaccact acctattgct attatttaaa atagggataa ttttttctct taaaaaatat
      541 cattatttgt ggtatggagc ccccgcattg atccggaagg cacggtagat ttgctcgaca
      601 aggatcagac gcatcaattg atgtggatac gtcatttttc caaaagagat ctgagcattg
      661 cttcttttca tgacggcttc gctgagtcct aaagagccgc cgataacaaa aacaaggtgg
      721 ctttttcctt ggatacctaa ttggtcgatt tgtttagcaa aagattcact tgtaggattt
      781 ttcccttcaa ttgctaaggc atagacgtat tcttgttctt tgattttcgc taagatccgt
      841 tccccttctt tttcttttac ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt
      901 tcgtcaggaa cttcgatcaa ttcgattttt gcataggcat tcagccgttt gacatactca
      961 ttgatacctt ggatcaggta tttttctttt aattttccta ctgagataat tttgatattc
     1021 atgttttctc tcctttggtc ttcatgctca ctttgtttat tataatcaat tttttcgtct
     1081 atatgtttat tttggctatt tttgttaaaa agatttcccc aacagttatg cacaacatcc
     1141 acaggtttat ccacagaaaa cagtgttatt ttaccttatt tcatcacatt cattaggcaa
     1201 acatacgttt tataagaatt tatccacttt atacacaggc tgtggatatg ttttatccct
     1261 tatttttcca atcctatccg cgtagtttta cacagtatct gtggaaaaag aaaatcaaat
     1321 attttcatct gaaaacaatg atttttccta aagtttttca taaaaagttc acagtctcaa
     1381 agtagactaa agtcaacaaa taagactata ctaacagtag ataaatgaat gtaattggag
     1441 gaggaacaca tggatagaaa aaatgtgaca ccaaagatga aaaaaagtaa aaataatagc
     1501 ctctggcgta agttaggtct ggggcttgtt ggagggatcg ttggaggact actgacagct
     1561 ggcatttttt atgcagcgat gggcactggc aacaatagtt ctactgctac aagcggcaat
     1621 cagaattctg ccggtgaaac agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc
     1681 accaaagcgg tagataaagt tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa
     1741 aaccaatcaa gcggttttgg cggactattc gggcagcaag aagaaagtag cagctcagat
     1801 gatagtaatt tggaagctta tagtgaagga agcggcgtga tctacaaaaa agacgggaat
     1861 actgcttatg tagtcacgaa taatcacgta gtagatggcc agcaaggtct tgaagtcttg
     1921 atgaaagacg gtacgaaggt aaaagctgaa ttagttggaa cagatgctta ttcagattta
     1981 gcggtgttga agatcaattc agacaaagtc gaaacagttg cctcttttgg tgactcaagt
     2041 gctctgaagg ttggtgagcc tgcgattgcg attggttccc cactaggatc tgaatatgca
     2101 aattctgtca cttcaggaat catttcttcc ttgaaccgac aagtgacaag tacgaatgaa
     2161 tcgaacgaaa cagtcaatat caatgcgatc caaacagatg cagcaatcaa ccctggaaat
     2221 tctggtggtc ctttagttaa tatcgaagga caagttatcg gtatcaactc aagtaagatc
     2281 gcaagtactt ctgaatctac ttctaatgta agcgtagaag gaatgggatt tgctattcca
     2341 agtaacgatg tagtgaatat catcaatcaa ctggaaaaag acggaaaagt gacacgtcca
     2401 gctctaggta tcacaatggt cgacttatct gccgtttcta ctcagcaaca agaacaaatc
     2461 ttgaagattc cttcatctgt aactaacggt gtcatcgtga caaatgtcca aacagctaca
     2521 ccggctgaaa aagctggatt gaaacaatac gacgtcatta ctaagatcga tgacaaagaa
     2581 gtttcttctg gtgtagaact tcaatctgct ttgtataaga aaaaagtggg agattctgtc
     2641 aaaatcactt actatgaagg ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat
     2701 caatcggcat taaaacaaaa tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa
     2761 gacttgtcac aacctcttta ttttggaaat gcttctccga ctacggcatc ctcaggcaat
     2821 actaagattc ctttttttgc tggagcattt ggcaaatgca attcacgagc ggaacagatc
     2881 attccatagc tatctacccc tcgcaaagtt cctggccaga tcatcattcc atctggcatc
     2941 atagctcctg gttttgcaac gacaactttt tgtccagctt cgatattggg tgcaccgcaa
     3001 acgatttgca atactgtacc attgtctact tctgtctcag taatcgataa atggtcagaa
     3061 tcaggatgtg ggacgcatgt ttttacgtac ccgacaacga tcttcggttc atgatcagct
     3121 tcaagctgtt cttcgaatcc tgcttctttg atcaatcgat tcaatttcgc taattcctca
     3181 tctgttaagc ttacttggcc gttcccttcg attgttagat gatcggagat atggaaaaag
     3241 ttccaagcaa caacttgacc ttgttcatct ttgatccgag caacattcca tttgcgttca
     3301 acagtgtttt ctgttccttt atcgtcggct ataataacca tcaaggtgtc tccgacgtgt
     3361 gctttgttat aagcaaaaat catgttcttt cctccattat cctttgctga gatttatctt
     3421 aacaaaaaat tgcattcact gcatgaaatt tttttcatgc tttatttccg aagaagcctc
     3481 ttctggaaca ccatttattc gtaacaaggg actgtgacaa gacttttgtc acagtccctt
     3541 tactgtttta gttaattgat taaaaaggtc gtacaagttc cacttgctta attacttggc
     3601 tgttcttctt cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat cacgtagctg
     3661 gctacacttc ctgtcataaa gcgttcgact gcatttagac ctgactgccc aaccatgatt
     3721 agatcgatct catatttttt tggcaatttg actgccatgg attcacgaac agaaccatat
     3781 gcaataattc cttctatatt tgtgtaatca acagatttcc catattcttt acattcttcg
     3841 atcatttcct tggctgtttc agtttgctga tcgactagat tttgatcaat aggtgcatag
     3901 cccataaagt tataaaattg ctgatcgatt acgtttgcta catatacttt cccattatta
     3961 cggcgagcca cttctaccgc ttttttgaaa gcagcaagtg cttgttcact accgtcgata
     4021 cctactaaaa tattcttata ttcttgattc atcttgaccc ctcctaacag atgtcagcct
     4081 tttgttacac tttctaggaa cgattcgatt tcctgttttg ttttacgatc tttattcact
     4141 agacgaccta attcttttcc gtcttggatc acgacaaaac ttgggatacc aaaaatgttc
     4201 cattcagcgg ctacatctat gtacttgtca cggtctactt caataaattg ccaatcagaa
     4261 aagtcatttt caatttcagg catttgcggt ttgataaaac ggcagtctcc gcaccagccg
     4321 gcagtaaaga aaaatacact ctttccagtt tctacgtagc ctgctaattc ttctaaacta
     4381 tttgggataa tcattctttg tcctctactt tctgtttatg tatttttcaa ttaaattata
     4441 cacctttctt tcgaactaac aaacattgat cctcttttta taaaaaaaat ctggcaaaaa
     4501 gcaatctttt gccagatttt atagtttcac tatcgcatta ttttccgtat acgatcgtat
     4561 ttactgttga tttatccaaa cctttcagtg tttgaatcaa catttctcgt gcagcttcaa
     4621 aatcgcgaat actgaacatc gtttgatgcg tatggatata acgtcccact acgccaatca
     4681 ctgtacttgg gattccttcg ttttgtgtat gagcagcacc tgcatctgta ccgccttttg
     4741 aaacaaagta ttgataaggg atattgtttg tttcagcgat atccagcaag tactcgcgca
     4801 aacgaggaag catgatcaaa cctggatctt ggatacgcat caatgtacct tctcctaaat
     4861 gtccgaatgt gccatttttc gtcactgtat catctgctgc agagcagtct actgcaaaga
     4921 acagatctgg cttgaattta ttgacagaag ctttagaacc acgcaagcct acttcttcct
     4981 ggacattcgc gcctgcaatc aatgtatgac ctaatgtttc attttgcaac gcttctaaag
     5041 cttctaatac cattgtacag ccatagcggt tgtcccatga tttactgata atattctttc
     5101 cgttggctgt tttgatcgtt tcagcgtaag gaacgattgt gtctcctgga aggacaccgt
     5161 aactcattgc ctcttctctt gattcaaacc cagcatcgaa caaaatatcg gttacttcta
     5221 ctgatttttg tccgcttgtt cctcgcaata aatgtggggg aacggaagaa gaaatacatg
     5281 gataattctt accagtagag gttttaagtg taaaacgctg agcagaaaca acgtaaggat
     5341 tccagccgcc tagaggtacg actttaaaaa gcccgttttc ttggatttgt gtcagcatga
     5401 atccgacttc atccatatgt gctgcgacca ttacacgggg tgcatccgct tcttcatgat
     5461 ggcgcagacc aaaaatgccg cccaaaccat ctaactgtaa ctcatctacc agcggttcca
     5521 tatgactttc catataagcg cgaacatctt gttcaaaacc gcttgttcct tgtaattctg
     5581 ttaactcttt gattcgttga aatgtttttt cttccatatg ctattcgtct aaatttcaga
     5641 ctaaactcct ctcagactta tattgatctg cggataaaat atgatttttt ctgcagaccg
     5701 atcgtattta ttatagctta ttttttctgg tagcggttat ttatttcaaa gaaaatatga
     5761 gagtgtggta aaataagcac aaggagtaac aagtgagaag aaggaggtat gtccacatgt
     5821 atgaagaaag cgaactaagc tattttaaag gcgggttagc agttggaatc ggtctaggat
     5881 tattgagcgg ggtagcttca acgatttggt atcatcgcaa aaaagtattg tcagcagacc
     5941 aagtacttga aaatatcaag tcatcttttc taaaagaagg tgcaatcgaa ggttcttgga
     6001 tctcttttga aaaggaacct attaggaaat tcgccgttcg gtcgagtgct tatcggggag
     6061 gtctttctcg aatagaagat ggcgaaactg tttattatga atttttagca gatgctcata
     6121 caggaaccgt attagaaatt tcaagaaaaa aaggtgcaac agattaatac gtcaaaaaag
     6181 tgagagaacg aatacgcacc cactattcaa aagagaggta tcatgtcctt cttttgtagt
     6241 gggtggtttt ttagatagag ctttccaaac atatattcca gtattttctt tttaaataaa
     6301 tgatatttag cgggaaaaac tagcatttcc tttgattatg aggtattttt aatttagctt
     6361 ttgttttcta tagttaagga gagtgaaaaa tggaacaaca agaaaaaatt gctgttctga
     6421 ttccttgtta caatgaggaa gcaacaattt caacagtcat tgcagacttt aaacgagaat
     6481 taccagaagc agatatttat gtctatgata ataattcgac cgatcgaaca tacgaattgg
     6541 ccgttcaagg tggagcaatc gtaaaaaaag aacctcgtca aggaaaagga aatgtgatcc
     6601 gccaaatgtt ctttgatatc gatgccgact attatttaat ggtagatggc gatgatactt
     6661 atcctgcaga agctgtacat ggtttgctcg agaaattacg ttctggtgaa gcagatatgg
     6721 tcattggtga tcgcctatca aatggaacct actttgatga gaataagaga cctttccacg
     6781 attttggaaa taaccttgtt agaaatacaa ttactagaat gtataaaaca aaaattcttg
     6841 atgtcatgac cggctatcga ggattcaatc gtatctttgt caaaagtttt cctattatga
     6901 gttctggttt tcaaatcgaa acggaattga cgatccatgc tttagataaa aaattcaagt
     6961 tcgttgaaat cccaatcgat tatcgtgatc gtcctgaagg cagcgaatca aaattgaata
     7021 cattctctga cggattcaaa gtgatcatga tgattattaa aatgtttaaa gattataagc
     7081 ctttgctttt ctttgggatt tggaccatct tcttcttttt atttggattg gtcacgggga
     7141 ttcctgtgat tcgcgaatat atgctgacaa gctttattac acgcatccct tctgcgattt
     7201 taagtactgg attgatgact ttagcacttc tttcattagt aacagggttg attttggata
     7261 cagtcgtcac gaacgctaag aaagaatatg aattgaatct atatcatgtt tatcatgaat
     7321 accacagtag aaagtaaaaa aaggatcaag actgcaaaaa taatttgcaa ttcttgatcc
     7381 tctttttaat taattatttc gccaaacgat agatggcatc tgcatagatt gctgttgctc
     7441 tgaacaagtc atcaagactc ataaattcgt ttgcttgatg cattgtatca gtatatcctg
     7501 ggaacatcgc tccataagca acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga
     7561 tgatttgttc gtagcctttt tctccagtgt ggtcttcata gacttgcaat aatgtagcca
     7621 ccaatggatc atccattggt acgtaatgtg gttccattac gcgagcacct cgagtaactg
     7681 ttgcgccttc ttgccccact gtttgttcga tgtcactttg caatccgtct actgttacac
     7741 cttttggaaa acggaaattc atattgatga agttcgcttc ctctgtacca ttttcttcaa
     7801 aagcaaatag tccagcattc attgtcaatt tgcccatttt ttcatcttca taagcgactc
     7861 ctagtttttc tccataaaaa tcttcatgga cataagcagc tgctacatgg ataaattgtt
     7921 tagcagatcc taggaattca tagttatcta agaacaatgc caagaaagaa cctgcattga
     7981 taccagattg tgggcttgca ccatgtgcgc ctttaccaac taattcgatt tttacatacg
     8041 tattgtccgc ttcaatcgtt ccgcttactg gatttgcttc gatgaattgg taaaatgctt
     8101 cttccatcgc gatagctgca tctgcacttg gtacttgaag tgctgcagtt gctgttcctg
     8161 gcaccatatt ttcgcgaagt ccggaaacaa aagatttcaa gacataatct cctgcattgt
     8221 cgcctttgaa gcgtaaagct aaagaaacat ttcctttttc accattgatg attgggaatt
     8281 cagcatctgg tgagaaacca aaatctggtt cttcttcatg ttcgaaataa taagccatat
     8341 cgccccagcc gctttcttca tcgctgccga ctacgaagcg aacttttttt gatagtttca
     8401 agtcaagttc tttaatgatc ttcatcgcat agtaagctgc catacttgga cctttgtcat
     8461 cgcttgatcc gcgtgcaaag atctttccat ccttgattac tggttcataa ggatctgtgt
     8521 cccagccatc tcctgcagga acgacatcca tgtgtccaaa tatccctagc gtttcgtctc
     8581 cttcgccaag atcaatatgc cctgcatagt tatcgacatt ttttacttta aatccatcac
     8641 gttcgccata agctaacata tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat
     8701 ctggtgttac ttttgagtca tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt
     8761 caagcaagtc ttctttacgt gcttctactt ctttttgcca atcaattgtc ataaagttaa
     8821 tcctccactt atttaagtct tcgtgttcat gataccacaa attacagaaa aaaatagttc
     8881 aaaaaatctg acaattcctt ttcttatctg tttttctcta aaaaaaatat acgctataat
     8941 gagtaaaagg aggtttttag atgctgataa acgaacaact agtgaaaaga gtcatcacac
     9001 ctcgtccaaa aatttcacat aaaggaacat ttggacgtat cgttttgatt ggcggcaatg
     9061 aacaatatgg cggagcaatc attatgagtg ctttagctgc cgtacacagc ggagcgggtc
     9121 tcacgactgt agtaactgat gaaaaaaatc ggagtgctct tcacagtcat ctgccagaag
     9181 caatgattgt agtttggacg gatactgacc agtttctttc cgtaataaag caagcagatg
     9241 tactcttgat tggtcccgga ttgggtactt cttctaagag tttggaacgt ttcaaacaag
     9301 tactagaaaa ccaatcaaaa gaacaatggc tggtgattga tggctctgct ttaagtttac
     9361 ttgctgctaa tcatcttttg ctaccctatc ccgaacaaac tgtacttaca cctcatcaaa
     9421 tggagtggca acggttgagt tctctaccga tacaagaaca acgggataaa gaaaacaaaa
     9481 agaagcaaca ggaaatcggt gctgtgctcg ttttgaaaag tcatgagaca aaaatttatg
     9541 gtcaccagca cttctacacc aatcctttag gttctgctgc tatggcaacg ggcggcatgg
     9601 gtgacacttt agctgggatg atcacaggat ttttagccca gtttcctcaa aatgaccaaa
     9661 cgattgcagc agctatttat cttcatagtt tcattggaga ccagctggca aaagaacaat
     9721 atattgtttt gccgacacaa atcagcgaag cacttccgaa atggatgaat tatttttgta
     9781 aaaaatcaat ggaaaacgag gcatgagtca tggagaaatt tacaggttat gtgcgagaac
     9841 ctttttatta tgaaacagat caaatgggga ttattcatca cagcaattat atccgctggt
     9901 ttgaagaggc tcgggtagcc ttattggtac atctagggtt tccctataaa aaaatcgaag
     9961 aatctgggat catcatcccc gttctggaag taaattgcag ctataaagaa atgatccatt
    10021 acggagaaaa agtgagcatc catccaacta tccaaaagta tacggggaca cggttggatt
    10081 ttagctacga aattgtggga actgaagaca aaaaattaaa aactactggc agtagtaaac
    10141 actgtttttt actttctgat tcgcaacgat tagtgaagct gagcaaagtc aatccggagt
    10201 tagatcagtt attccaagcg tacgcttctt tggatgatgt aaattcttaa taaagaccgt
    10261 ctaagcattt ttcttgtatt gtgtcgagta gttcgtataa tgaaaagaaa aaagcaggtg
    10321 atccccatgt atgtcgttaa agtaatgcac ggttacatcg ataaaactgg ctgtcgcact
    10381 cgggaaaaaa acttagacaa tctgctcatt ttcaaagaca aaaaagaatc cgaagctttt
    10441 gctaaaagaa tcggcggtcg tgtcaaaccg atccaagagg tacgaccaga ttgattcaat
    10501 atttttcttt tcgaggctct aataggcatg cctatcaggg ctttttttgc agactgtgtt
    10561 tttatgccca cacgatttgt aagcgattta tgttataata gattatatct gcatagaaag
    10621 gaaggttaaa tggttatcaa taatttgcaa cagcaagctt atgaatctat acgtaaacaa
    10681 atcatttatt ccgagttagc acctggaagt aaaatctccg ataaagtgtt agaagaacaa
    10741 ctagctatcg ggcgtacacc gattcgagaa gcactgatcc aactgcgcaa tcaagagctt
    10801 gtaaaaacca ttccacagtc cggcacttat gtttcaaaaa tcgatatccg atctgcttca
    10861 ttagcccgtt atacgcggga aaagttggaa aaaccgatcc tgcaagaatg cagtgcgaaa
    10921 atgaccaagc aagatcaagc taaactagaa aaaatactag aacaaaccga tcaggcagta
    10981 atcgctcaaa ataaaaaagc tttttttgat ttggatactg attttcaccg aacttgttat
    11041 gaaattgctg gaaaacgaga aatttgggat tggctagaaa gttatagtac gcgtttcaga
    11101 tggttacgtt tgacgatttc agaattagac tggggtcgag tattagatga acatcagaca
    11161 atgcttcaat caatgattga tcataacttt gatgaagtcg gatttctttg cacgatgcat
    11221 ttacatatga ttatcgagga gcaagaatac gtgattcata attaccccga ttattttgaa
    11281 gatattcagg acagacccat caaataagag gttttggaat cagcgttttg acctgagtat
    11341 tagagaaaga aacggcaaaa tagttcctca tattttgccg ttttttttga gctaataccg
    11401 agggtcagct gattgcacac cgtttattga gaggttgtga aaaagctgcc ctgcatcaag
    11461 taataagaac agccaagtaa aaatagcttc ttatgttttt aacttggctg gacttattac
    11521 cgcagatgca agcttttgaa caccgttttc caagaggtcg tgagcctgcc gttcatctcc
    11581 gagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tgttgaatat tttgacttgt
    11641 gaccgaggag ttgacaggag aacatcgttt attcggaaaa aaaggactgt gacacgactt
    11701 ttgtcacagt ctctttattt gtaatctaaa tcatactccg tacatctttt ttctattttt
    11761 tttatttctt ctaagatccc ttggctacga atttcattat atggccaaag taagactgcc
    11821 tctaatacat aaggcgaaaa atcgctttga ttcatctctt ttgaaaaact catcgctgct
    11881 atctcgctca atgcacgaat gtttgattga tgtgtataaa aaggcaaccg ccacagcaca
    11941 attttttcgc tttgcgtata aattccctta acagaagctt cgatgtggtg aaatagttca
    12001 tgtccaataa tcaaagcttg cgctgtttct ttagagatcg aagctagtcc tgaacatttt
    12061 aatttttcta acggctccgt catcaatcga atcccttcat cttctgcata agttgcaaaa
    12121 gtgattcgtt cgtttatttg ctctgattca caggaaacga ttggaacagc catttgttca
    12181 caaatttctc ctacatcggc aaaagggtac ttttttttga tgctacatgc cgtttcgata
    12241 ccacaatcga ttgattgttg tataagaatt ttctgcttgg acaaaggaat ctttttccaa
    12301 aataaatctc gccgaaactg gtagaatccc caaatttctt cggggatctg ttgtaattcg
    12361 tttagtaact cttctaactt atgtttgttt gatatttccg gcaacgacga tcacttcttc
    12421 ctccggttcc atagtagata cttccagatc catcaaaagc tgtagttgtt ccagatccgt
    12481 cgctgtaaaa tcagagactc ttgctcctaa gcagagataa aattctggtc gggcaatcaa
    12541 ttcagtttga taaactgcca tatcttccca aagttgttcg acagctgtca tataattggc
    12601 tgcagttgtt ttcatacatg aagcatgtcc acgttggacc ttgataaatc ctttttgatc
    12661 gatgatccga agacttccag cattttttga acgattctgt tcgccgcata cataaaagaa
    12721 aggtgtattt tccaataagc aaacttcatt tttatttaat cgcatatcct ctgcagcaag
    12781 ctctagtgct tcttctgttg tgatctcctt ggttagatcg gttgctttca cttccgtaga
    12841 accagtagcg atagccgtta cttttgaagt ctgtggatcg atttctacgt ggacttcgat
    12901 cgattctggt gtagcgcctg attcaatcgc tttattcatc gcttcatttt tcaacgatcg
    12961 tatatcttct ttacttggcg aagggatgat acgttctaca acatctcgga ccattgcaag
    13021 tgctacacca atagaagaaa tcacttcggc gttttcagga atcgaatatt tcacacccat
    13081 tttattacta aagtaagtaa tcaatgaggc agcgccgccc cctacaccta caagagaaat
    13141 ctgatctttc tccaagtgat acttgtctgc caattctaaa atcaccggtt caatcttagc
    13201 ataggatttt tccatgatct gttctgcgat gtcttcaacc gtcgtatgac aataatcagc
    13261 taatgcttgg atggcttttc gtgcagatgg gacatttcca taagaaaaat gttcttcttg
    13321 cactaatcca aggacattgg ctgcacatgt atttgtaata gtcacttctt ctccgtcttc
    13381 catcactacc ttgacgtaat cagcagggtc tccttcttta ggagagaaaa attcaacctt
    13441 aggacctttg attttttctg tctccgtaaa tactgcataa tctagaccgg caatatgtgc
    13501 agaacgtgga ccgacatcga tgattcctga ctgattagcc cgtaccatcg aaccgcctgc
    13561 cacacctaga acccgaacat ctaatgaaga gatatatgtt gggtggccac ctacaattga
    13621 ataatcgatt gctggacgtc catttttaat gaccccgata ttggttgttg ttccgcctac
    13681 ttcaaaataa acaccattag acgctctcaa gtacatcaaa gagcccatga ctgatgctgc
    13741 tggaccggaa agcatggtta aaacaggtcg ttttttcatt tcattgattt ccattactcc
    13801 gccgtctccg cgcataatca tcaaagaaac attgacccct gcttctcgta cggaatcttc
    13861 tgtagaggtt gccgtatcta gcatttttgg taaaatagat gcattgattg ctgctgtccg
    13921 agttcttctt gtcaatccat ataattttgt gatgtcagaa gccatcgttg tagggatcga
    13981 ttttaccgat gctgcttcat aaacaactcg ttctggttcc ccattatcta ctccaaatgc
    14041 catagaagaa accaagacct ccgctctttc tctttctaaa gaagaaatcg tttctgaaac
    14101 acggtctaca tttaagtgtt tgacaggtaa gaatgcattg acaatctcta tttttttctt
    14161 attccctaaa tcgatatcgt tcaatctagt ttgtcgtttt gctaaaaaac cttctaatcc
    14221 accttttgcc atcccgatta cgccgacttt tgctacgtct ccttcaatca gtgcattagt
    14281 cgcttgtgtg gtagaatgtg caacaaacac aacatcttct ggactgatat tattttccag
    14341 caaacaattt tgaaaagact gaacgacacc tgcggctact ccacggacat catcgtgggt
    14401 tgttttcact gaagattttc cgatgatttc gtgcgtcgca ttatcgattg cgactgcttt
    14461 cgtatgtgta ccgccaacat caattcccat tcgcacaaaa cgtttcattt tcttttcctc
    14521 cattcatcta atcgttttta tcgacgtttt gtttactagg cgttcccgta caataagaac
    14581 gtgatcaggc ataagatcgc tccgatgatc caaccagttg ggatggattt cttcatatat
    14641 tctcgagaag ttacttttgt ataacctaat ccccaagcaa cccaagattg tgtaatatct
    14701 aaatgctgag gagcaatcgt tgtgattgca aataacggga acaaaaaggc tggcgacatc
    14761 gttggattaa cagctaatac gacagctaaa atagctgagc ctgaaccgac tagattcatt
    14821 ggaccgcgga aaaatcctag tggtgtcaat atagcaaata cgattgccaa gactaatggt
    14881 gattgcggta tccaatctcc aaaaatagct gaaaaataag gagaagcata cgcagctgaa
    14941 ttattgaaca tcgctaaggt catcaagaaa ccaaccattg gcgcaacatc tactgcgcca
    15001 tctgtaaata atttcgcaaa ctgacgacag atttctttat aacctccgcg caattttcca
    15061 gtaaacagca atgcccagat acctgctaga ataaaaccta agatgattgg aagatctaaa
    15121 agaacgaccc ctaaaactgg taataatacg gctaaccatg agatcattgg tgcgtcgcca
    15181 ccttcagctg gtacgtttgc tgccattgca tagcgtttct ttttgttcat tgatatatta
    15241 gcgaccgtta gtaccacaac taagcttaca atcatcccaa tcataccaat ttggaaatag
    15301 tcattataag tatagctttc agccgctgga ttgaaaccag cataaatcgt ttgatattgt
    15361 ttgaaattga cgatattagc aaagattcca gccataatcg agcccataaa tgagaacagt
    15421 gcaaccggta ctgaaatacc taatgacatc aagattggca acacaataac tgcaatagaa
    15481 atcactggtc cgattcctgt catcgacatg aatataacag cggttacaat acatagtaag
    15541 ctcatcgtga ttcttggctt gtcccctcca agttcaacta cctttcgaat cagggtagct
    15601 gcgattccgc tatctactag aacacgcccg aaaaaggcac caaagaagac atttaccaag
    15661 atcgatttgg cgtactcagc tggccccgtc gtatagatat gcgtcaacac atcaattact
    15721 ccttgccctt ccatcgccgg gtttggtgaa aacgtgtttc cgactagcgc taatcccatc
    15781 catacagtag ccatgatggc aaagccgacc atcaagttgt agccttttac acagtaccag
    15841 accatgaaaa agaatgagat aactaacaaa ataccaataa taatatcact catgttattc
    15901 ctccgtccat tttgtttttc tgataagcaa actaatatat cagtcaaact cattctaact
    15961 tatgtagtta gcgctttcat taaagggtga ccataaatgt tcagttttca attaaaaaac
    16021 cttaattatg ctattttaga cttatacgtt attgtcctgt ttcatttgat ggaggattgt
    16081 aaataaaaaa agaggaaaga acataacttt tcgttatctt ctttcctctt tataattgtt
    16141 tagcttattt agccaaagct tcttttgctt ggtcagccaa tgcagtaaat gctgctgcat
    16201 cgttaacagc caagtcagct aacattttac ggttgatgtc gatttcagca actttcaaac
    16261 cgtgcattaa ttttgagtag ctcaagccat tcatacgagc tgctgcgttg atacgagcaa
    16321 tccacaattt acggaagtcg cgtttctttt gacgacgatc gcgatatgcg tagttgtatg
    16381 aattcattac ttgttctttt gctgttttaa ataaagtgtg ttttgaaccg tagtaacctt
    16441 ttgctaattt tagcactctt ttacgacgtt tacgagttac tgttccacct ttaacacgtg
    16501 ccatgtataa ttcctcctaa taaaaataaa tcttcagatt ctgttatttg tacttcttat
    16561 ttcatttttg ctagttgttg gcgaatacgt ttgaagtcgc cactagaaac catgcttgct
    16621 ttacgcaatt gacggcgttg tttttttgtt ttgccgtgga aacggtgact tgtaaacgcg
    16681 cggaagcgtt tcaatccgcc tttaccagta cgtttgaaac gttttgctga tccgcggtgt
    16741 gttttttgtt ttggcatgac tatatttcct cctcaaaatc tttcgttatc cgactatcgg
    16801 ctgtccaaca gccagttagt cattcaaatt acttactgtc gtttttcggt gccagcgtca
    16861 agaacatgct gcgtccgtcc attttcgctt tttgttccac tgttgcaata tccgcagttt
    16921 cttcagctaa gcgattcaag actttttgac caatttcttt atgggtaatg gcacggcctt
    16981 tgaaacggat agaagctttc actttatcgc ccttttccaa gaacttacgt gcattacgaa
    17041 gttttgtatt gaagtcattc aagtcgatcg taggacttaa gcgaacttct tttacattga
    17101 tcactttttg ttttttacgc gcttcgcggt ctttcttttg ttgttcgaaa cggtatttac
    17161 cgtagtccat gattcgcgca actggtggtt tcgcaccagg ggcaactaac acaagatcca
    17221 agtttgcttg ttctgcaata ttcaatgctt ctgcttttgt cttaactcct aattgctcac
    17281 catctgaacc gatcaatcgt aactctcgtg cacgaatgcc gtcgttaacc atcatatcct
    17341 ttgctatggt cattcacctc caaatattat gagagaaaga aaagcttacc gaataaacgg
    17401 caataaaaaa acgagttctc ttacagaacc cgcgcgtact tcacctaata gaaccagtga
    17461 aaactatcta gcccagtgac attatcaact aaggcgagaa gcgggtgctt ctgcttttat
    17521 ttctcaacca tcatagtata gcaaaaacta atctttttgt caatgttttt catacagttt
    17581 gtcttccttt tctgaaaatg attagattag cagactttct agcagtgatt cccgcttctt
    17641 tgctatacta aagaaaaacg aggtgaaccc acatgaaaaa aatcgaacct attccacctt
    17701 ctcttcctct tcttacaaag ggagagtttt atttagaaga tgaggagatc ttctctggtc
    17761 ttctgatcaa cgggggtgac caaagctatc tcgatgccaa aaatatggta ttaagagaga
    17821 gtcatatcca aaaacttacg atgcaaaaaa cagttttacg tcattttgaa tgcagtaatg
    17881 tcatttttga aaaatgtgat ttttccaatc tggagtggat tggtggcagt ttccaccaag
    17941 tcgtgtttca tcaatgtaaa ttaacaggta cgaattttgc cgaaagttat cttcgagact
    18001 gtactttcac tgattgtatt gcttcgatgg cttcctttag tagtacaaac ttgaaagctg
    18061 tctccttcga tcattgtcaa ttagaagaca gcgaatttta tgaggtcact tggaaaaacc
    18121 tttttttgtc tgacaatcag ttgaccggtt cgaactggtt tcgaacttct ctgaagggat
    18181 tagactttac gactaatttc ttcacagcaa tcgctttatc tcaagattct ttggcgggac
    18241 taatcgtcaa ccaagagcaa gcacttgtga tcgcccaagc ccttggtttg cagatcaaag
    18301 actagatcaa cgaatccgcc gccacagtgc aataagcatc caaatagata agaccaaact
    18361 gatgatcaag gcgattcccc atccgaaaac ggaatgggta aagggtaact ggagattcat
    18421 tccgaagaat cctgtcacaa tagttggaac ggtcaagatc agcgaccaga ccgtcaaaaa
    18481 tttcatcgtg tcattcaagt tgttattcag caaattatta taagtgccag agagctggtc
    18541 taaaatctgc gaagctaagt tcgtcatttc aactgcttgt cgtgcttcga tcagcgcatc
    18601 gtccaactct tctttttcct tttctgacaa cttgcgataa atagccaatg ccttgatttg
    18661 ttctaaaagt actacatttt gttttgttcc tgtaactaag taaaccaaac cgatctctaa
    18721 atctgaaagt tgcagcagat tcttgttcgt tgttttttct cttaatttca gattaagccg
    18781 ctgacgttca ctattcactt cctcgattaa aggaaaaaag gaatcagaaa tcaaaaacaa
    18841 tgtacgaaac aataaactgt atacactcat gtctggcatt cgttccaaaa gcgatcgaat
    18901 tagtcgaatc acgtactcgg tattcggcgt ggtaaatgta aacaaaccgt cttcttttaa
    18961 aataaaagcg ataggactag tttcataatg attatcgact ttttgctggt gcggaacatt
    19021 tgatacaagc agtaacgctt catcaaaaga atcgtactct acgcgcgccc gttcattttt
    19081 atctagagaa taagtcagca tctcgtcact aatgccatat tcttctttta agtttttaac
    19141 agctagcaca tcgttttgct gtacattgat ccaaaaatta cggtcttgat taaaatagtg
    19201 tttttcccgc atactctatc ctcctgacat ttgtttccag tataaagaaa aaagtcgctc
    19261 aaataaaggt ttacccttta tttgagcaac tgtaaatcaa atggctactt tggtttggcg
    19321 aatctctgct aatttttctt cgatcatttt caagacttct tcttttgctt gaggatcttc
    19381 cacaaaatta tagcggtctc catcgatttg gatttttgga ctttctttgt attcttcata
    19441 ccattgatcg taacgtctgt tcaattcttt gtaataatcg tatagacttg gatcaaagga
    19501 caattgttca tacgaacgac cacgtttttc gatacgctcc aacatcgtat caaaagatac
    19561 acgaatatga actaataaat ccggatgttt tttatgggca gcatagggaa gttcttccat
    19621 catgttttcc agcagttcat catacacacg aacttctgtt tctgttgctc ttcctagatc
    19681 tgcatttaga tggaaaagca gtgaatcttc ataaatcgaa cggtccaata cattattgtc
    19741 atctttcatt gcttgtttga tgcttgcaaa acgtttattc aaaaaataaa tttgcagcaa
    19801 aaaagcatac tgttcagggt ttgcataaaa taagggcaag acttcattat catcaatcga
    19861 ttcataaaat gcctgactat caaagtgttc agccatcatt tctgttaaac ttgattttcc
    19921 ggcaccaatt gtacctgcta aaacgatcac gctcatttac ctccagctca cttcatcact
    19981 aaatattgtg tttaactcca acctttttaa atataccccc aatatctagt agtgtcaatt
    20041 gtattttttt ctagtcaaag aagaaaaaaa gtcaaaaaat tcttaagaaa aaagtgtttt
    20101 ttctttagaa aacgtattct tagttttact tctgagatgg ttttagtaaa ataaaggagg
    20161 aatgtttttt gagaccctta gtggacaaaa aaattcgtga agcagcgcaa ctcgttttgg
    20221 gcgctttatt ttttgcttaa aaatcacttt tacgtgagaa ggagttagat tatggcaaac
    20281 tatggccaag gagaatcaag cggaaagatc atattgatgg gcgagcacgc ggttgtttat
    20341 ggagaaccag cgattgcctt tcctttctat gcaacaaaag tcaccgcatt ccttgaagag
    20401 ctggatgcaa tggacgatca actggtttct tcctactatt caggaaattt agccgaagct
    20461 cctcatgcat taaaaaatat caaaaaatta ttcattcact taaaaaaaca gcatgacatc
    20521 caaaaaaact tgcaactgac cattgaaagc acgattcctg ctgaacgtgg aatgggatca
    20581 agcgctgcag tcgccacagc agtcactcgt gctttttatg attacttagc atttcctttg
    20641 tctcgtgaaa tactattaga aaatgtccag ctttcggaaa aaatcgccca tggtaatcct
    20701 agtggaatcg atgcagccgc tactagcagc ttgcagccga tttattttac aaaagggcat
    20761 cctttcgact acttttcttt gaacatcgat gcttttttga ttgtcgctga tacaggaatc
    20821 aaaggacaaa caagagaagc cgtcaaagat gtcgctcacc tctttgaaac ccagcctcat
    20881 gaaactggac aaatgatcca aaaattagga tacttgacga agcaagcaaa acaagcaatc
    20941 attgaaaatt caccagaaac gttggcacag acaatggatg aatcacaatc acttctggaa
    21001 aagctgacaa tcagcaatga ttttcttaat ctattgatcc aaacagcaaa agataccggg
    21061 gcattgggtg ccaaattaac tggcggtgga cgcggtggct gtatgattgc attagcacaa
    21121 acaaaaacaa aagcccaaga aatcagtcaa gcacttgaag atgcgggtgc tgctgaaact
    21181 tggatacaag gattaggagt acatacctat gtttaaaggc aaagcacgcg catataccaa
    21241 cattgctcta atcaaatatt ggggtaagaa aaatgaagaa ctcatccttc caatgaacaa
    21301 cagcctttcg ttaacattgg atgcatttta tacagagaca gaagtcatct tttctgatag
    21361 ctatatggta gatgaatttt atctagatgg caccttgcaa gacgaaaaag caacaaaaaa
    21421 agtcagtcag tttcttgacc tttttcgtaa agaagccggc ctctcactaa aagcttcagt
    21481 aatcagtcag aatttcgttc ctaccgcagc tggattagcc tcctctgcca gcgggctagc
    21541 tgctttagca ggcgcttgca atactgctct taagcttgga ttagatgatc tctctctttc
    21601 aagatttgct cgacgcgggt ctggttcagc ttgccgaagt attttcggtg gtttcgtcga
    21661 atgggaaaaa ggccatgacg acttaagttc ttacgctaag ccagtccctt ccgattcttt
    21721 cgaagacgat ttagcaatgg ttttcgtttt gatcaacgac cagaaaaaag aagtgtccag
    21781 cagaaatggg atgcgtcgga cagtcgaaac atccagtttt tatcaaggct ggttagattc
    21841 cgttgaaggg gatctatatc aattgaaaca agcaatcaaa acaaaagatt tccaacttct
    21901 cggagaaacg atggaaagaa acggactaaa aatgcatgga acgactttag ctgcccagcc
    21961 tccattcact tactggtctc ctgacagctt aaaagcgatg gatgcggtgc gacaattgag
    22021 aaagcaaggc attccatgct actttacgat ggatgctggc cctaatgtca aagttttagt
    22081 ggaaaacagc catttatctg aggtacaaga aacatttacc aaattattta gcaaagaaca
    22141 agtaattact gcacatgctg gtccaggaat tgcgattatt gaataaaggg agatttttat
    22201 gattgaagta tctgcaccag gcaaactcta tattgccgga gaatatgcag ttgttgaaac
    22261 aggccatcca gcagttatcg ctgcagtcga tcaattcgtg acagtcactg tagaatctgc
    22321 acgaaaagtc ggaagtatcc aatctgcaca atatagtggg atgcctgtac gttggacaag
    22381 acgcaacgga gaattagttt tagatattcg ggaaaatcct tttcattata tccttgctgc
    22441 tattcgcttg actgaaaagt atgcacaaga aaaaaacatt cttttatcct tttatgatct
    22501 gaaagtaaca agtgagttag acagttcaaa tggccggaaa tatggattgg gttctagcgg
    22561 tgccgtgacc gttgcaacgg tcaaagcctt gaatgttttt tacgcgttga atttatctca
    22621 gttggagatt ttcaagattg cagcactagc caatttagca gttcaagata atggttcctg
    22681 cggcgacatc gctgctagct gttatggtgg ctggatcgct ttctcaacct tcgaccatcc
    22741 ttggctccaa gaacaagaaa ctcagcattc tatcagtgag ttacttgccc tggattggcc
    22801 aggtctatcc attgagccat tgattgctcc tgaagattta cgtttattga ttggttggac
    22861 gggtagccct gcctctactt ctgatttggt cgatcaagtt caccgttcga gagaagataa
    22921 aatggtggct tatcagcttt tcttaaaaaa tagtacagaa tgtgtcaacg aaatgatcaa
    22981 agggtttaaa gaaaataatg taacgttgat ccaacagatg attcgaaaaa accgacaatt
    23041 actgcatgat ttatctgcaa tcactggggt cgtcatcgaa acgcctgctt tgaacaaatt
    23101 gtgtaattta gctgaacagt atgaaggagc cgcaaaatct tctggtgcag gtgggggcga
    23161 ttgcggaatc gtaattgttg accagaaatc tggcattctt cctttaatga gtgcatggga
    23221 aaaagcagaa atcactccac tgccgttaca tgtctatagc gatcaaagaa aggaaaaccg
    23281 atgaatcgaa aagacgaaca tgtttcacta gctaaagcct ttcatgataa acaaaaaaac
    23341 gaatttgact tcgtacggat catccataac cctttgccgc aaatagcagt ttctgatgtc
    23401 gatctaagta cacaggcagt tgggtttaca ctaagcagcc cattttatat caatgcgatg
    23461 actggcggaa gcgaaaaaac aaaaaaaatc aaccaagatc ttgcaatcgt cgcaagagaa
    23521 gcagatttga tgattgccac tggttcagtc agcgctgcct tgaaagtccc ttctttagcc
    23581 gatacttata cgattatgcg acaagaatat cctcacggga aaatcattgc caacatcggt
    23641 gcagggactt ctgtcgaaag agccaaagaa gctatccgat tgtttcacgc agatgcctta
    23701 cagattcatt tgaatgcacc tcaagagttg gttatgcctg agggagaccg tgattttacc
    23761 aattggaaag tacttattca agaaactcaa acagccattg acgtccccct catcgtaaaa
    23821 gaagtcggtt ttggcatgac aagagaaacg ctcaatgatc tagctgcttt aggagttcat
    23881 acagttgata tcagcggtcg aagcggaaca agcttcaccc agatcgaaaa cgcccgtcgc
    23941 tccaagcggg aactgagtta tttagctgac tgggggcaat cgacagtctc ttcattgctt
    24001 gaagcaaatg aagcagacac ttctatggaa atcctagcct ccggaggtat ccgcaatgcc
    24061 tatgacatat ttaaagccct ttgtctcggc gcaaatgccg tgggtacttc aggaaccgta
    24121 ctgacgcatc tgatgaatca cggcgtagaa gaaactatta tactgatgaa gcaatggcaa
    24181 gaagagcttc gtctgcttta tactatggtc ggcgcaacga ataccgcggc cctacaccaa
    24241 caatctctga tcttttccgg accagtgaag gattggtgcg aagcaagagg gatcgactta
    24301 gtgaagtatg gaagacgcac agaaaagagc gtgggacaaa agtaaagaac acttttgtct
    24361 cacgctctaa acacgtacaa acggcggaag taaaagcaac tccttcggaa ataagctgaa
    24421 attcacaaaa atttgaaaag caattttcgt gaattccttc ttatttcttg gagttaaatg
    24481 cttctgtccc agcctctttt ttcgaataaa tgatgtttaa tcagctgtcc ttcggtattc
    24541 gttcaatccg ctgatttcac aacctctcca ctaaacagtg tactaaagct cttttaaggt
    24601 tgttttccga tataggcaag tatgcctccg tccacataca agacatggcc gttaacgaaa
    24661 tcagatgcgc tagaagccaa gaagacagat ggtccagcca aatcgattgg ctcgccccag
    24721 cgtgctgctg gcgtacgtcc aacgataaat tgatcgaatg gatgacgttc accgttttct
    24781 tgtgtttcgc gtaatggagc agtttgcggt gtagcaatat aacctggtcc aattccatta
    24841 cattggatat tgtattgacc gtattcagaa gcgatgtttt tcgtcagcat ttttaatccg
    24901 ccttttgccg cagcataagc actgaccgtt tcgcgtccta attcactcat catggagcag
    24961 atattgatga ttttaccgcc gcctttttcg atcatatccg gaataactgc ttttgaaacg
    25021 atgaatgggg catttaaatc cacgtcgatt acttggcgga agtctgccgc atccatttcg
    25081 atcatcggtg tccgtttgat gatccctgca ttgttgacta gaatatcaac tgaacctaca
    25141 tcttctttga tctgttgaac catcgcttga accgcttctt catcggtcac atcgcagaca
    25201 taaccttttg catcgatgcc tgcttcttta taatgcgcta taccttcatc aactgattct
    25261 tgattcaagt tattaaatac gatttttgct cctgctgtag ctaaagagcg tgcaatctca
    25321 aatccaatac catagacagc accggtgact aatgctactt ttccatctaa acgaaacata
    25381 tccatactaa attccatcat tttctctccc ttatgcttat tttaattgat ccatttgcac
    25441 catatccatg tctgtatagg tgatattttc tccgcacatt gcccagatga atgagtaatt
    25501 gcttgtacct acacctgaat ggatcgacca gcttggcgaa ataatggctt gttcattgtc
    25561 tacaactaaa tgttttgttt catctggttt gcccatcatg tggaacacac gtgtatcttc
    25621 attggcataa tcaaagtaga cataagcttc catacgacgt tcatgtgtat gacaaggcat
    25681 cgtattccaa gcgcttcctg gttctaaaat cgtataaccc atttgtaatt gacagctttc
    25741 gcatacattt ggatggatgt attggtagat tttacgttca ttcagtgtca ggccttcacc
    25801 agtctccatt ggttttactt ggtcgatact gatttttaca tttggatatt tatgatgagc
    25861 tggaacagaa ctaatataga atttagctgg gttttcagca tcttgagaag aaaatacaac
    25921 atgtcgtgtt tcttttccga tatagtatcc gtcttgtttt ttcatttctt ctttttgccc
    25981 atcgatctcg atgaatcctg gtccaccgat attgatcaca cctagttcac gacgctctaa
    26041 gaagtaatca acacctagtt ctttgtctaa tgtgatttct aattcttttt cagtaggtgt
    26101 cactccacca aagatcattc gatcgttgtg cgtataagtc aaactgattt tccctggttc
    26161 aaataccttt tctactaaaa attcttttct caattgttcc gttgaataat gacggatatc
    26221 ttctggactg tgtgtataac gtgtttccat ttcttgcatt ttcttctact ccttctatcg
    26281 aatgattttt ccagagccgc atgctaaaaa ttgtgcgact tcttcttgac taaattggtt
    26341 gcagtcccca tggatcgtat gcttcaaggc agaagctgcc gtagcaaaat caatgatttc
    26401 ttgcggagac atattggata aaatcccatg gagcacgcca cctgcaaaag catctccgcc
    26461 acctactcga tccacgattg gggtgattct atggacttgg gattccacat attcatggtt
    26521 catccacaat gtccccttca attcattttc acttgctgaa tgaacggtac gcttggtcga
    26581 ataaaacacc tgaatattcg ggaacttttc ctgcattttt tgataataat aaattagagg
    26641 ttgttcttct gcttctggcg ctttctcgat acctaacaaa taaagagcat ccatttttcc
    26701 agctgagcag atatctacta aaggtaaaag ctgatggatt acttcgctgg cttcttgctg
    26761 tgtccataat tttccacgat agttgatatc gaagctgatc aggcagccag ctttttttgc
    26821 agcttctatc acctgtttcg tcattttttg ccatttttta gacaaagctg gggtgattcc
    26881 tgaaacatga aatatatcta ttccttggaa taactcctct agcgaccatt ccaaatgatc
    26941 catttcagca aaacttgatc cggcacgatc atagatcact gaggcggcgc gttcgccact
    27001 accagtttcc atataataag tacctagtct tgatccacca gataatacct gtttcgtatc
    27061 tactccataa cattgcaagt gctttttgac tgcttttccc aatgcgttat caggtacttt
    27121 tgtagcaaaa atcgcttcat gtccgtaatt agctaaagaa attgctacgt ttgcttcccc
    27181 tccgccataa tgggcagcga atcgttcact tgaatcgata cgggttccct gctctgtgga
    27241 caatcgcaac atgatttctc cgagtgtcac tacttttccc ataagcagct acctcttgat
    27301 ttctttcatt tttgctgcat actgttgcgc gacttccgtt actttgtcaa aatctcctgt
    27361 agctgcgggt gctaaaaggt tcccacctac tccgactgtt acgacaccag catcgaacca
    27421 ctctttcata ttgtccagac tcacaccgcc agttggcatg atattgactt gaggcatcgg
    27481 cgccttgaag gcagaaatga tacttggtcc ataagcgctt ccaggaaata gtttgacgat
    27541 atctaccccg cttttcagcg ctgttttgat ctcggtaata gtcatacatc ctggtaagta
    27601 aggaatttga tacagattgc acatctctgc cgtttcttga tcaaaggaag ggctaacaat
    27661 atactctgcg ccagctaaga tagccaagcg agctgtgaca gcgtctaata ctgtgcctgc
    27721 gccaatcact atttctggtt ggtctttgta gaatgacaaa agttctttga ttacttgatc
    27781 tgcttgaggg actgtaaaag tcaactcaat accagttaga ccacctttga tgattgcgtg
    27841 gcaagcattc aaagcttctt ctttgctttt tccgcgtacc accgcaatca ctccggcgtt
    27901 tttcaatcga gataaaatat caattttttt catcttctcc acctatttcc tatttagaaa
    27961 ttttatttta taacttggaa atctataccc atattatacg gcagaaaaga aaaattgcaa
    28021 acgctttttt cattattttt gaattctttg ctcgatttcg ctttttgttt gcttgagggc
    28081 actgattatt tgttcttttg tctcatctgt taaacgatat ttcggcatac ttacactaaa
    28141 tgcaccaatg atttgttcgt ttttcacgat tgaggctcct atacaaaaaa tatcacgttc
    28201 gatttcttca tcgtcaaatg cgatgtgggt ttcttttacc gttttgattt cctttttcaa
    28261 ccgtaatgca ttggtgatcg tatattctgt gtaagggatc aacggatgct tttcaagata
    28321 atcagtgtac tgctcttcac tgaattctgc taatacggct ttgcccatcg cagaattgta
    28381 taatggtcgg gtgattccga tttttgaaga catgcggatc gtttgatgtt ttggatctag
    28441 tttgttcacg tacaagatct cattgttgtc caatacccct aagtggatag tctcatcgat
    28501 cttttgctgc agctgttcta aaaacggtaa agtcaactct accaaatcga tttgttcaat
    28561 gtttttattc gcgtagcgaa taagctttgc acctaaacga tagtttttct ctgaattttt
    28621 atttacgtag ccgatcatca acaaagtatc tagtattttc aagactgtag aagaggtcat
    28681 tcccacaccc tttgcgatcg tctgcaaaga gacatcttgg ttttccgcta aatagtctag
    28741 tattttagag gctttgatca atacagttcc ataaggcttt ttattttcca tataatattc
    28801 tcctatttct ttataagaaa aattatagcg gaaataccat caatttaaaa gagatatcca
    28861 gatataaact accatttctc tttacaggca atttaatgtt gaagatccgc ttcgttttta
    28921 catcaaactt tgatataaag caatgatttc ttctactgtc gtttctcttg gatttcctgg
    28981 tgtgcataca tctcgcaaag catcttctgc aattgctgga atatctgctt cttttacacc
    29041 taattcagaa atggtttctg ggattccaac cgctttgctc aaccgatcga tctcaccaca
    29101 agccgcttct cttacatctt ctaaagacat ttcagctgct cctttaatcc ctagaacaag
    29161 agcgatctca cggtactttt ctccagtata ttctttgtta tatttcatga cagttggcaa
    29221 aagtgctgca cacgctacgc catgaggaat gttgtaccat gcacttaacg ggtgagccat
    29281 cccgtgtacc agtcctaaac cgacatttga gaagcccatt cccgcaatat actgtcctaa
    29341 tgccattttt tctcgcccat tttgatcgcc atctacagaa gcttccaaag aatggccgat
    29401 gatttcgatt gcttttaaat gcagcatatc agtcatttcc caagcgcctt ttgtgataaa
    29461 cccttcaata gcatgcgtca tagcatccat acctgttgat gcagccaatt ttttcggcat
    29521 tcccatcatc atatcgctgt cgatgaacgc gacgattggg atatcatgag gatcaacaca
    29581 aacaaatttt cggtgattta cttcatctgt gatgacgtag ttgatcgtta cttctgctgc
    29641 tgttccagaa gtcgttggta tagctaatat aggtaaacat ggatttttcg tatcagcaac
    29701 accttctaaa ctaatgacgt ctgaaaactc tggattcgtc aaaataatcc caatggcttt
    29761 tgccgtatcg attggcgaac ctccaccgat tgcgatcaaa cagtctgcct ctgcctcttt
    29821 aacaaaagcc actccatctt ttacattttg gatcgttgga ttaggtacga tcccgtcgta
    29881 taaagcataa gcgattgctg ctttatctag aagttctgtt acttttgttg ctataccgtg
    29941 ctcgatcaat cctttgtcag taatgacagc tgctttgccg aatcctcgct gctgaaattc
    30001 agggacgatc gactcaatcg ctccttttcc aaaatacgat gtttcattta aaatcattcg
    30061 attactcatt ttctattcct cctaatttgt attatttttc acaataaaaa cgaaagaacc
    30121 ccaagcaacc ggttgcccag ttgcttgttg ctacagttca aagactggca tcaattccca
    30181 actgactgga gaggcatctg gatttgtctc catgatatct tccatgtact tccaccattt
    30241 ttgattgata gatgtatttg ccattttttc ccataacttt tcatcttcga tcactaagta
    30301 accaaataac aaacttgtac tcggatcaag atagatcgaa tagtcgatga ccccgtgttc
    30361 acgaagtgct gtacgcattt ctggccaaag ttcatcatgt ctttttttgt attcttcttg
    30421 tttttctggg taaactttca tgcaaaaagc ttttttcacc atgtcagaca cctgctttcg
    30481 tatgaacttc ctccaaataa cctgcttttg gagtgacacc aaacgcatct gctaaatgcc
    30541 acaggttttc gtccgtgatt gtttgcaaga ttggtccttg cgctttgacg tacgtataga
    30601 cttctgctgc cttttctgct gtctcgatca aaccaaagac ttcatccatg tcttttcctg
    30661 acccgtagat tccatgttgt ggccataaaa ccaatcgagt ttctttcatt ttttcagctg
    30721 ttgcttcccc aatctcattt gttcccggaa ccatccacgg aatgatggaa attccttctg
    30781 gaaaaacaac taagcattcc gtacacattt gccatagcgt acgactaaag gaacgttcgt
    30841 ctaattcatg cgtaaaactc atcgctaata gatgactagc atggcaatgc ataacgatcc
    30901 gattatctgg gtctacttct aaacgggcga tatgactcat gaaatgactt ggcaactcgc
    30961 ttgtaggaag gcattcgttt tccagccccc acaaaagttc cagtgttttg ccatcttcag
    31021 caactcgaac gattccaaga ttctcggcag gttcgctcgc tacattttta aaatatcttc
    31081 ctgaaccagt tacaataaaa tagcatcctg ctaaacggct ggcatcaaaa atcatcgaga
    31141 tgcggcggat cactttttca ggatccaaga acggtgtgat ctcttcttct tttagtaaat
    31201 aagaaatatt cccgccattt ctttcatccc atcccaagcg ataaagattc gacgtcgttt
    31261 cgatcatttc tttcacataa ggcgcttgta aaatatctat ttttttcaat atagccacac
    31321 tcctattttc ttaattccaa cacgtctttt tgatattcct ggacttcttc cagccaatcc
    31381 aaaccaactg gaacttgatt tttgtaacag aaataattcc atacatcggc atacgggaaa
    31441 tctttcaatt cttccgtata agccattctt gaagtaaagt caaaagccag ctccatttct
    31501 ttcaatcgct caactggttc cagcatagct ttcagcagtg ctttttgtgt atttctcgta
    31561 ccgatgaccc aagcagcgat acggttgatc gttgcatcaa agaaatccag accgatattt
    31621 gttttttcca acaattcgtt acggactaat tctttagcga tttcctgcaa ttcatcatcc
    31681 atcaaaacca catggtctga atcccaacgc actggacggg aaacatgcaa catgattcct
    31741 ttgctgaata aagctaatga agataattta ttggaaatca cttctgttgg atgaaaatgt
    31801 ccagcatcta gacaaacaag tttgtcacga gtcaacccgt aacccatata aaattcatgg
    31861 gaaccgactg tataagcttc agcgcctaaa ccaaatagtt tgctttctac tgcatcttgt
    31921 acataccgtt catctaactc ttctgtaaaa atttcatcga gtgattccat taaacgttcc
    31981 cgtggggtaa aacgatcaat cggattatct ttcatcccat caggtaccca aaagttattg
    32041 atacttgttt gcccagtttc tttcccaaag tactccgcga ttttgcggct tcttttcccg
    32101 tgttcgatcc agtattcacg aacttcttta tctggatgag ccaatgtaaa gccgtctttc
    32161 atcattggat gcgaaaagaa agtcggattg aagtcaagac cgatttcttc tttttttgcc
    32221 cactctaccc atttttcaaa atgtttcggc tcaatttcat ttagatcgac agattcttct
    32281 gtatccaaat agatagcatg aaggttcaat ttgtgtttgc ccggtatcaa cgaataagcc
    32341 ttctccagat cttgtctaag ctcttcaggt gttcgtgcgg ctcctggaaa attccctgtc
    32401 gccatgattc caccggtcaa ttcgccatcc ggtgtcaaga aacctttgac atcatctcct
    32461 tgccagcaat gcatagaaat tttgatgtcc tgtatctttt ctagtacagc atctgtatct
    32521 acaccgatag ttgcatattt ttcttttgct tgttcgtaat ttctttcaat cgtgttcata
    32581 cgattactcc tttgatttta ttcacgtacc attccatcgt gttttgctta ttttcatctg
    32641 ggtaaaaagt ttcacatgga aacgactgtt tgattacatt tcttgcttgt tctatattcg
    32701 aaaactgacc tgtactgagc atttgcatgg caacattccc gattgctgtg gcttcactag
    32761 gtcctgcttc tacaatgatt ccggcataat ctgctgtcaa ttgattcagt agacgattat
    32821 tcgatccgcc ccctacgatg tgcagctttt ccaatttcgt tccagttaag tgttctactt
    32881 tgatcagctc ttgtgcgtaa caaagagcaa gattatcata cacacaacga gcgatagctc
    32941 ccgatgagac agggactggc tgctctgttt caaaacagta ttgctgaatc tcttctatca
    33001 tatttggggg attcaaaaat cgagggtcat tgatttccat cacttgctgc agcggttttt
    33061 cttttgcagc aagctctgcc aattccgcat aactgtactc gtactcttgc attcgggcaa
    33121 cttcctggat caaccacatc cccatgatgt ttttcaaaaa acgcaccgtg ttatgggcac
    33181 cccattcatt cgtataattt tcagaaaaag cattgcgaga aacattggcg atctttgttt
    33241 caatccctaa aagcgaccat gtcccactgc tgatataacc ccaacgttct ccttgacctg
    33301 gcgttcctaa tacagctgac gccgtatcat gacttgctac tgtgataaag gtcacatctg
    33361 gcaaatcata ttttgaaaat tgttcttttt gcagatctcc tagtatcgtt cccggctctg
    33421 tcaatggtgg aaataattcc ttcgagacac cgattttttc aagaagttct atttcccatt
    33481 cttttgtccc tgcattcagc agctgcatag tagatgcatt agttttctcc gtcaccatgt
    33541 taccagtaaa gacataccct aaataatcag gtatcagtaa caatttatgt gcttttgcca
    33601 aattttcttt ttcttcagca aacaattgga acaaggtatt gaaaggctgg atctgaatcc
    33661 ctgtctgctc atacaactta tctagagaat agatttcaga aaactgttct actgctccat
    33721 tggtacgctt gtcccgataa gcaatcggat catttatccg atccccttgt tcatcaatca
    33781 gacagtaatc gactgcccat gtatcgatcc ccgcaaaaag gcgatcaatc cctgtctgct
    33841 ttaatttttc aaatccaatc aaaatctctt tgataagata atcgatcttc cagcgatcgt
    33901 gaccgtcttt gttccgaaag ccgtttttga accggtgtac ttcttccaag atcaaagaac
    33961 cttttgcatc cctcgtactt ttcattagtc gtccgcttga tgcaccgata tcgatggcaa
    34021 caaatgttgg attcatttct ccacctcttt tactttattg ttgattagca ctttattaat
    34081 aaagcgttat catttattga caatgctatc ataatacaga atcttatatt ttgcttcaca
    34141 ctattttggt atataattgc attatcttga caagttggga gtgtttacat ggattactgg
    34201 gaaaaagcca accactcatg gtcggaagac tcaaaaagag tgatcttgac gcctacacaa
    34261 agaagcagga atttattttt ttatatccaa gaaatcggtt acttcaaagc accaatccct
    34321 tactatactg aacgagcaca tttaccttct tacttgataa agtttacttt gtctggcaaa
    34381 ggttcgctta catataatga taatcagcaa cagctaaaag ctggagactt attttttatt
    34441 gactgccaag aatatcagcg ttatcaaacg atcagcgaag aaccatggga aatggattgg
    34501 atccatttga atggtcagaa cgtccaatca ttttatcagg agttcacaaa agacggccat
    34561 aacaccttcc attcaacagg agatccatca gaaaacaaga tccaccattt gatcacagaa
    34621 ttgatccaca tgcaacaagg cacgaatgcc cgtaccgatt tccagtcctc tgtagctatc
    34681 cacgagttgc tgaatgaact gattttgcaa aagtatcagc tagatttcga agaagaggat
    34741 attcccgcct acgtttacaa actgaagcaa tacttagacg aacatttcaa agaaacgatt
    34801 acacttgatc aattagaaca acaattctta ttgaataaat atcagctgag caaagatttt
    34861 tccaaattta tcggttctcc accaatcgat tatttgatca gcaaaaaact ttcttacgct
    34921 aaagaccttc ttcggtatac agaatttacg gttcgaatga tttccgaaga aatagggatt
    34981 gagaattttg cttactttag ccgcctgttc aaagcaaaaa caggactatc gccgagagac
    35041 tttcgtaaat tcagctgatt catataatat gtacgtcatt ttttcaaaat attttgaaat
    35101 atatagataa aaataaaata accgcagttt ctgcttctat gtaaacaaca gcagctactg
    35161 cggttatttt ttagtgatca ttctcattgt tcatcttcaa aatgttcata atgaaccaac
    35221 tgtttctgtg tatcacgttt caaaaattct ttaggcgtta tgccgaactc tctcttgaat
    35281 gctttcagaa aatgcggata agaagaaaaa ccacttttac tatatacagc actagagctg
    35341 cggtattctc gcaacaagtg cttggcatag atcagctttt tcttcattac atactgatga
    35401 aacgtaaatc cagtgtactt tttaaattct cgtgtgatat ggtatttgct gacaaaaaac
    35461 tgcttttcca tttcctctaa agaaagcgga tgagataaat tttttgagat atattgtaac
    35521 atttgttcca cacgctcatt aggaactaca gcattctgtt cttgcgcact ctcttctttt
    35581 tgtacaactt gattgagaaa aatcaagtat tctaccagag cttgttcata ttttatgtct
    35641 tcaccgtaaa tttttttatc aggaggatgg tcgataaatg ataatttttg cttcaatact
    35701 tcctgttcta tttgcaatac acgactcctc ttctctccca ttggttgaaa acaagccgat
    35761 agattagacc atggcgtgga acgactttct agaaaagaag gggtgacaaa gatatagaca
    35821 cgctcgaata catctgaaga ttggcggacg atacgatgca aatcattgga atggatgagc
    35881 agcacacttc ctgctttgat cgtaaattgt cgtccgttca aataaaaagt cgcttctccc
    35941 tttaatgtag catggatctc atagaaatca tgatgatgat agatcgtttt attttggctg
    36001 acgacatctt tgacatgata gatttcatac tgatcagaaa ccatcgtatt gacttgttca
    36061 ccgctgattt tcttttggag catgtgccgc acctcctttt gttcacttat gcacaatttt
    36121 attataccac gttttttgta atcaaaaaga gctgctttta tatactctta taaaaaagaa
    36181 gagacgtggc atcttctagc cccgccccct ccattctaaa tattagttaa ccaacgtctt
    36241 ctttacatta ttcaagaaga cttcttccat aacgaatccc atcatctttt gtttcttcaa
    36301 gaaccacata caggtacgga cgattcttca tgatgatttc tttgattttt gtgtactcga
    36361 tcacaccgtc tcccaaattt accggcacga ttttttcctg ttcgacacga aaatctttca
    36421 aatgaaccgc acatatcttt tcaccaaaac gttcaaaagc ttcttctacc aattggactt
    36481 gttcttgata atcactactt gtgatcaaat tggtcggatc taaaatgatt cctagatagt
    36541 ccgaatctac tgcttggatc agctgctcta cccttgctaa agaatacacc ggatgattca
    36601 aacctggttc tatccccacc atcatttggt atttttctcc agcttttacc aagcgacgga
    36661 taacggacac agcttcagca aatgcttcat ctgtgaaatt ttcttccgta tattggattt
    36721 ccggcaaaac acatccagtt tccgaagcaa ccatcgacgc accaaaggaa gaagcatagc
    36781 gaagatactg ctcgaacttt gtcagcaact cttgtcgtat cgttaaatct ggatgaatca
    36841 tattgatata gcaacttaaa attcctacgg tcacttcttg cttttctaaa actcgtttga
    36901 cataatttcc catcccagaa ttgatctcat ttgcgccaga aggtatttca ggaaacgaaa
    36961 gacctaaggc gagctgcagt gtatgaatcc cttgttcttt tgttttttct gcaagatctt
    37021 ctatcgtttg gacttcggaa agatcatgtc ctctaattgt aatgttcaac ataactattc
    37081 tcctaactca gcatattggg tagccacatg acgatttgcg ggaagtaagt aatcaaaaac
    37141 aagacgacta agataacccc ataaaaagga agaagcggtt tcagcattgc ctctgctttt
    37201 acgcctccta cacttgcacc gacatatagt cctgtaccta ctggcggcgt gatggagcct
    37261 acacacagat tcatgattga aaacaacccg taatgtacag gatcaacccc tacacttgtg
    37321 acgattggta aaaatattgg tgtaaaaatc aagatagcag gcgccacatc cataaacata
    37381 cccaccagta ataacacaag attgactaat aacagaataa taaatttatt atccgttacg
    37441 cccataatca acgaactaat tgcctgagga ataccggtaa acgccatggc gaaagacatc
    37501 atggaagaag tagccaataa gaacatgatc gtccctgaca ttgctactga ttgaaacaac
    37561 atattaggta tatcttttag ttttaccgtt ttatagtaaa acatagcaat caacagggag
    37621 taaatgacag ctatcgctga agcttctact gctgtaaata tccccgtcaa gataccaccg
    37681 ataatgatga caatcaatag aaggctagga atcgcctctt ttattatttt tccagcatga
    37741 gccatctgtc ctttttcgac aacagggtat ttattctttt tagcaacgat gaatgctact
    37801 agcatgattc ctagcgccca caaagcgcct acgagataac ctcccataaa caaagcagag
    37861 atcgaagcac caccagcaac aagtgaatac atgataaaag cagtacttgg aggaatgacc
    37921 atacctgtcg gagctgacgc gatattgacc gctgtcgcaa acttccggtc atatccttct
    37981 tccacttgct gagggatcaa cactccgcca atcgcggtag aagcggcaat ggccgagcta
    38041 gagatggaac caaataaagc attccctaac acattcgtat gagccaaagc ccccggcacg
    38101 cgcccgacga acaacatcgc aaaatccact agtttcttag ctatccctcc attattcatg
    38161 ataatgcccg agagaacgaa aaacggaata gcgatcagcg aaaaactatt gatacttccc
    38221 accatttttt gagctgaact aaaagcagaa acatcaaacg gtataactaa caataaagtc
    38281 atcatcgatg agaatgcaat acttatagca atcggcatcc ctaatacaag caacacaaca
    38341 aatacaaaaa ataacactaa tccggcttct attgccattt tattcctcca tttttatatt
    38401 ttttcttgaa ttgctggctt gttgcttaat gtatcgatta gattgatcag gctatagata
    38461 atgatgagta cccccgatac aggtactgac agataaacat atcccatagg aatacttaaa
    38521 gaaggcgaga tctgccccat tgtcaatgtg acagcattcc atccgccata caccatgatc
    38581 atcgcagcaa ataccagaaa taaactttga acaacaactt ctaaaatttt actttttttg
    38641 ccagttaatt tttcacttaa aaccgttacg gctaaatgtg ctttttgtcc cacaacatac
    38701 gcggaagaca acatggcaaa ccagacaaga ccgaatcgaa caagctcttc cgtgaccgta
    38761 tttggatttc ctaaaaccgt tctggaaaat acttgccata aaacaacagc gaccatccca
    38821 acaagcaaaa cagctcccag gatctctaat accttgttta aaatcatttt catcacagct
    38881 acgctccttg ctcttggatc ttttgataaa ggtctttgta ggtaggatcg tttttgaata
    38941 tatcatgaag tggggcaact gcctctcgaa acggtgtatt atctacatca ttgaattgga
    39001 ctccatgttc ttcttgtgct aaagcttttt cttcttcaat tgcttttgca aaagcttcaa
    39061 tttcaaaaac cgttgattct ttcgctgctt cttcaatggc tttttcctgc tcatcagtta
    39121 gctgttcttt gatggcatca ttcatgatga tgatatctgg cacacgtgta tgctcatcat
    39181 aggaatagta tttagccaca ccaccatgtc cagctgtata caagacaaat tcattgtttt
    39241 ctgatccgtt gatcaaattg gattgaagag acgtgtacac ttcatcactt cccataggta
    39301 cgggcgaacc gcctaaaagc tctaccattt tgatagcggt ttcgctttgc atcacacgga
    39361 tcttctttcc cttcaaatct tccggcgtat ggatcggacc gtctaccata taaaagctgc
    39421 gctgaccaga atcataatag gtcaatccgg taaatcctaa atttttggtc gattggtaga
    39481 ttggctccat tatttcttca ttatccatca ccttaaagaa atgttcttca tcattgaata
    39541 gataaggaac tgaaaaaatc gaataatctt tagagaaact ttccaacgct gaggcactta
    39601 cttttgtgaa atctactgct cctgactgtg tcaattcgat cagttcacgt tctccaccca
    39661 gttgtccatc tgggaaatac tggatctgta cggaaccatc tgttttttct tttactaaac
    39721 gcccaaattc attcattgca tcaattactg gctggctgtt gcttgcataa gcaaagcgca
    39781 aggtaactat ctcatttaca tctgcatctg cgacagttgc cacactagaa ttatttccgc
    39841 agccgcttag acctagcaga cctaggctaa gcgaacttgc tgcaaacagc agtgatgttt
    39901 ttttcatatg ggtttccttt cttttaacaa taagagacga gcaattctgt caaacaaagc
    39961 accgttaacg actgtccata aggcattgct gtgattccga tattccgata aaaatctaaa
    40021 ctgtccccca ttcctgtccc gattgaaacg ttttgaactt ctcctttttc atcgatttgt
    40081 tcaagcaatc cttggatagc acgataagcg acttctttat attcttgtgg taaataacgt
    40141 ttatggaccg ctttcaaaat accgtaagca aatcctgcag ttgcagaaga ttctacgtaa
    40201 gaagaaggat catccaacaa cgtatgccat aaaccgcttt cagactgata agtttttaat
    40261 gcacgcactt gtgcttcaag tgtttcgatc aataatttgc gcaatgcatc ttctttcgat
    40321 aattctaaga tttcaatgat ctctggaata gcaatcgtca accagcagtt tccacgagcc
    40381 caaaatgctt cggcatagtt atgatttcct tcaaacgtcc agccgtgata ccacaatcct
    40441 gttcgtttat ccatcaagta tttgatatgg atcataaact ggtaacgagc ttcttctaaa
    40501 tattctgggc gattcaacag tttcccgatt tttgccaaag gaagcactgt catcatcaat
    40561 gtatcgtccc acaactgatt cttattttcc ggtccatatg tggcatgctg taacccgtct
    40621 tcatttgtcc gaggcatgtc attcatgacc cattctgccc attgttctaa ataaggcaaa
    40681 tagcgtgaat ctttcgtgtc ttcatacaag taagccatag tcaatagcgg tgccattgta
    40741 ttgacgtttt tgggcggagc gccttcttcc atacgatcct caaaccattc attgaccact
    40801 tgataagcgc gaggattttt tgtcatctgg taatttttat aaataccata tagaccgaca
    40861 ccttgtggcc aattccacac tgtccagctt ttgtcatcta ctttcagtcc atcaaagtcc
    40921 aacaaaaatt caccattatc gtctttgatc gttgtcaggt tttcaacgag caggtcaatt
    40981 ttttctttca ctaattcttt aggtacaaaa atctcttctt taataatcat ctttacgcca
    41041 cctttttaaa cgctttcatt ttatatcctt aatatagcag taataccgtc cactataaat
    41101 atcgcttttt gaacaaaaac tgtctaaaat tgacctaaat gttaaataac aaaagtaact
    41161 ttcagttact tggaaattaa aaaaagaact tggagaaaca acgttttcca agttcttttt
    41221 catctatttt cttatctttt ttctacgctt tcttttgctt atccgttgta agcatcaatg
    41281 atgatctgtt tcaattcaga gatcaatggt tctttcggat tagctgtcgt acattggtct
    41341 tcgtaagcta attcagccat acggtctacg gttgtatcta gtgtttcttg tgtgactcct
    41401 tgagctttta gactcatgtt gatgccgaca gattttccta attcataaac agccgttgct
    41461 aaagcttcga ctaattcctc tgttgtttcg cctttcagac ctaagaattt tgcgatatct
    41521 gcgtaatctg tatctgcacg gaaatagtca tatttaggga acatcgcatg tttttgtgga
    41581 tcttttgcat tatagcggat aatatgcggt aaaagaatcg catttgtccg tccatgaggg
    41641 atcccgtatt ccccgccgat tttatgtgcg actgagtgac aaatacctag gaatgcgtta
    41701 gcaaacgcca taccagccat tgtagaagcg ttgtgcattt tttcacgtga ttccatatca
    41761 ggtgttttta ctgatttttc taaatagtca aagactaact tgattgcttg taggctcaat
    41821 ccacgtgtat aatctgaagc catgactgaa acataggatt cgattgcatg tgttaacaca
    41881 tccatcccgg tatctgctgt gacagatgct ggaactgaca tgacgaattg aggatcgacg
    41941 attgcgacat ctggcgtcaa tgcataatct gctaacggat atttcacatg tgtttcactg
    42001 tcagtgatga ccgcaaatgg cgttacttca gaaccagtcc ctgatgttgt cgggatacat
    42061 acgaattgtg ttttttctgg tttgtcgatc ttatatgtcc gtttacggat atccaagaat
    42121 ttttgttttg caccaaagaa agatgtgtct gggtgttcat agaacatcca cattccctta
    42181 gctgcgtcca ttgcagaacc gcctccaaga gcgattactg tatctggctt gaagtctgcc
    42241 atcagtttcg ttcctgcgta tacggtattt gtcgatgggt tcggttcaac atctgagaag
    42301 acttcgatct tcacatcatt tttacgtcgt gccaatactt cacgaactgt atcgcagtaa
    42361 ccgaattgga ccataccagg gtcacaaacg atcatgacac gttcaacgtt ttccattttc
    42421 tctaagtaca atagagagtt cttttcaaag aagatttttg gcggtaattt gaaccattgc
    42481 atgttatttc tccgtttcgc tacagttttg acattgatta agtttacagc agagacgtta
    42541 cgagaaacag agttcttacc ataagaaccg catcccaaag tcaatgacgg aatcatttcg
    42601 ttatagatat caccgatgcc gccttctgca gatggtgtat tcactaaaat acgacaagct
    42661 ttcattcgaa gaccgaattt gacatgcaac tcttcgtttt ctgaatggat aactgctgta
    42721 tgtcctaatc ctccaagatt caacatacct tcacacaaat caaacgcatg gtccgtgttg
    42781 ttagctttga tcatggcaag aactggagaa agtttttctc ttgatagcgg ataatctggg
    42841 cctacgcctt ccaattcagc aatcagcatt ttcgttcctt ttggtacact gattcctgcc
    42901 aattttgcga tttccatcgc agaatgtcca acgattgatg gattgaccgc atattttcct
    42961 tcgttcatca ctgcatcttc taatttttgc aattcatctg gttttacgat ataaacttgg
    43021 tgtgcttgga attctgcttt tactgctgca tagatttctt tatccacgat cacagcttgt
    43081 tcagaagcac aaatcattcc attatcaaat gttttcgaaa cgatcaaatc gttcactgca
    43141 cgtttaattt ttgctgtttt ttcaatataa gctggtacgt tacctggacc tacacctaaa
    43201 gctggttttc ctgtagagta agcagacttg accattccag ctccacctgt cgctaagacg
    43261 atcgcgatac ctggatggtt cattagcata gaagtcgctt cgatcgaagg atgttcgatc
    43321 cattggatac aattttcagg agctccggca gcgatagcag catcacgtac cactcgtgct
    43381 gcttctgcag aagatttttg ggcacttgga tggaaagcaa aaacgattgg atttcttgtt
    43441 ttcaacgcga tcattgattt gaaaatcgtt gtggaagtcg gattcgttgt tggagttact
    43501 ccgcaaacca caccgactgg ttcagcaatt tcaatcaacc ctttttggac atcttcattg
    43561 atcacaccaa ctgttttatc atgtttgata ttatgccaaa tatattcgga cgcatacata
    43621 tttttgattg ctttatcttc gtaaacaccg cggcctgttt cctctacagc catcttcgct
    43681 aatggcatat gttgatccaa agctgccatt gccatttcat gcacgatatg gtctactttt
    43741 tcttgatcaa agctttccat ttctttcaat gctgcatttg ctttttctgc caaatcatca
    43801 atcattgctt gtacgtcaat tgtcttcgtt ttttcttttt ctattacttt agccatcatt
    43861 atcctcctaa tgggtcatgg ttatttgtta aataattcac atgtatatca tacaatattt
    43921 atttttctat gtaaacactt ttttgtgatt tttttcacag ttatttttaa atataaaatt
    43981 gctttattta caggtgtttg tatagaaaaa tatataaagt tatttattat tatttatatg
    44041 acacacccga ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg
    44101 aataaaaaat gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac
    44161 ttatttttct tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc
    44221 aactgtctca gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac
    44281 agtccctggt ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca
    44341 gtctaaaaca actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc
    44401 gatcgttacg actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg
    44461 ttgtttcttt tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac
    44521 aagcattggt aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact
    44581 ttagcagaaa aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta
    44641 gaagcttttt gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc
    44701 aagcaaatta tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta
    44761 taaattgtgg taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg
    44821 gatataggca cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg
    44881 gaaatcatgg gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt
    44941 gccatttcga gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac
    45001 accatcgatc aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg
    45061 gatcaacggt gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga
    45121 taacccgccg attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg
    45181 acgaagatct tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt
    45241 agcatgagct ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac
    45301 gtagtcgtaa ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc
    45361 caatttattt tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa
    45421 gtgatttttg aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac
    45481 ttgaaagccg cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc
    45541 gccagcttct tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa
    45601 aataacgcca gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa
    45661 tgttcctact ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc
    45721 taaacgcgcg ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt
    45781 cattcatttc cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt
    45841 caagtgcttt ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta
    45901 tccttttttt atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac
    45961 atggtatatt ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt
    46021 aaaacgccaa gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct
    46081 aatccctaca ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt
    46141 tgccaccacc atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg
    46201 tagttcagga ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg
    46261 tatttcttgg acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa
    46321 agatgcattt cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct
    46381 gatctcgatc tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag
    46441 ctattcttat tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac
    46501 acctaaagta ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca
    46561 acttttctgg ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg
    46621 tattggttat ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca
    46681 attgccaaaa gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca
    46741 ccgtttatcg agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca
    46801 acattcatgt cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac
    46861 agttaatcat tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac
    46921 cgcgattggt acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac
    46981 taggccgaat agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc
    47041 cagtaacgcc atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg
    47101 gctgctgcgt acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac
    47161 agtttgaccg acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc
    47221 taaataataa ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg
    47281 caataaacct cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc
    47341 gtgttccgct ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac
    47401 aaacagtagt tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac
    47461 aataaagatc tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg
    47521 ataaaccgaa cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa
    47581 aatcatatct gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac
    47641 gttacttgct ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc
    47701 acccgtacgt tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata
    47761 gacagagaga gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga
    47821 tcctgaaagt gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc
    47881 agtcaccaca tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt
    47941 gattgctcct ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg
    48001 taagtctttt tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata
    48061 accaacagca atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg
    48121 ttctacatcg cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga
    48181 gatgtatttt tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg
    48241 aagctcgttt actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc
    48301 aacagccctt acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc
    48361 ttgctcgacc gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt
    48421 cgtcattgaa tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg
    48481 catttcattt tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag
    48541 cgtttcaact gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc
    48601 gtatgcaagt gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa
    48661 ggaaatcgaa gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa
    48721 tactaaagcc gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg
    48781 gacctgtaaa ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat
    48841 ttctgtagcc atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca
    48901 tcctttaaat accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc
    48961 tgcatctttt tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg
    49021 tgatgcttcg atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat
    49081 atcacccact accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt
    49141 tgccgtttgt ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg
    49201 gacaatcgcc aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa
    49261 atccccatgt tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat
    49321 gatcgttgta aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc
    49381 ctcatcgaat ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa
    49441 cccagtatct ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc
    49501 ttccatccta ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac
    49561 atcctttttt tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa
    49621 atttaaagtg cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa
    49681 ggaggatacc atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt
    49741 tgttagaagt acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa
    49801 tgcgtatgga tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa
    49861 ttgaataacg taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga
    49921 tgtaacgaaa acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta
    49981 tgatggaaag ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga
    50041 gcttatcgag agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt
    50101 caaaactagc ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa
    50161 acgatagcgc tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa
    50221 ggcactaact aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc
    50281 taccactttg aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt
    50341 gatttctcca ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac
    50401 agccacgatt ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt
    50461 caaacggtag aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg
    50521 gaatgtacct aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat
    50581 ctcatcaagt aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt
    50641 ttctttcgca tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg
    50701 aggcaatggc atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa
    50761 gtcaatgatc cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc
    50821 accaaaagag attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca
    50881 cgtatctcct tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt
    50941 ttctccatat aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg
    51001 ctgtaactct tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc
    51061 caaaacaagt aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc
    51121 cggcaagtca aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt
    51181 gcttcatcat tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta
    51241 ttttttgttt tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat
    51301 actagtaata gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt
    51361 caaaatgtgt caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca
    51421 tttgaaaagg gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg
    51481 atgcggatga tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa
    51541 tccatccaag aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt
    51601 attggattga tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg
    51661 aaagtcagtc aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg
    51721 ccccgtgaga tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt
    51781 ggagtcgttc gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt
    51841 gcgcttgatc caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt
    51901 ttgggaaaaa cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc
    51961 cgtatcgcca tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta
    52021 cgtcatcctg caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc
    52081 aagccaaatg cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg
    52141 gaaaaatcat tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta
    52201 gttaccgata attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa
    52261 cgaggaaaag tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa
    52321 tccgcctatc ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg
    52381 gtcgttgatg aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc
    52441 gtctacgatg tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact
    52501 cctgaagaat ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa
    52561 attctctcaa aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc
    52621 atcattgctg taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg
    52681 attggcttaa ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc
    52741 ccgctatttg gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg
    52801 ccgattttac gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg
    52861 gcaaaaggca tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct
    52921 atgccaacga tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca
    52981 cttgcctctt atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat
    53041 taccaaccag atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct
    53101 gattggcttc taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat
    53161 gaagaatgaa acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga
    53221 gttgttcctt ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg
    53281 gaatcacttc agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata
    53341 cagataaaaa taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga
    53401 tgatgaacgg agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta
    53461 cattgaacct tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat
    53521 ttgagaaaag atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct
    53581 ttcttgttcg aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa
    53641 atgtagcgga tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg
    53701 gttatgacgg cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga
    53761 ttggacttgt ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta
    53821 cggatggacg gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc
    53881 caccatatga tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa
    53941 aagaagcctt aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact
    54001 acgaagcaga taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa
    54061 accactattt tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt
    54121 ctattatttc caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat
    54181 ttctatctat ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc
    54241 taggagacgt aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc
    54301 ttctctagcc atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat
    54361 tgttactgta tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat
    54421 ccaagtagac aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg
    54481 actattcatg gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc
    54541 gttagttgtt gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg
    54601 agatatcatt atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc
    54661 attgccaact gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg
    54721 gctagatcca gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg
    54781 accatttgtt ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc
    54841 tgattcttcc ctccttatgg gcaaagagtt gcttttctta gctatttatc cattccgtat
    54901 actccaactt catactgcca acgggttcat atctgcatcc accgactaag tttttttctt
    54961 atccgctaag gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca
    55021 tgatctctaa atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg
    55081 gcgtggcttt catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca
    55141 tcatcaaaac tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac
    55201 ttgccatcct catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag
    55261 gtttgcggtt cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact
    55321 tggcgatacg atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt
    55381 gccaataaga aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga
    55441 tactgctatt catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca
    55501 tgttcggcaa tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt
    55561 tcgtatctgt atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg
    55621 actccggttg attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga
    55681 catttccaat tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca
    55741 ctaacaacga tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta
    55801 cataaacccg tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata
    55861 taggaacaaa ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa
    55921 agagtctcct ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact
    55981 tcactcataa tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt
    56041 gtaaaccact cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct
    56101 cagcttttca aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc
    56161 ttcaaattgt ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt
    56221 tttgattgcg actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc
    56281 gtaaagctac tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca
    56341 agaaaaaggg tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt
    56401 ccactacgat cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac
    56461 tgataaaaat ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct
    56521 cctttttctg atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc
    56581 acttaaattc agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga
    56641 ggatctttgt ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt
    56701 aattggtaac aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca
    56761 acaacaccaa tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac
    56821 ctgaggatac gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc
    56881 ttctactgcc tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat
    56941 ttgatacttg cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat
    57001 ggtaacaaaa aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac
    57061 ttaagagtcg aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa
    57121 ggattcggaa attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga
    57181 ataataacat ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg
    57241 agaataagac aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt
    57301 ttaaaacttg gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg
    57361 ccaagacaat cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg
    57421 tctgtttggt acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt
    57481 tttacttacg atatctacat aacggataat tggattttca atcgagcgat aagaagagtg
    57541 gacgattgaa aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc
    57601 tttttcgaca tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg
    57661 tgcgtaattc aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt
    57721 attcccgtca taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt
    57781 tttataatga tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa
    57841 aggccaaatg tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa
    57901 cgcacccgta atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt
    57961 gacgaccatc cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg
    58021 aatcaagcgt tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag
    58081 tgtgataatc ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat
    58141 gaatttatct tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc
    58201 agcaactgct aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc
    58261 aaagacagct ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata
    58321 ccaatagttg ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt
    58381 caatgtagca gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat
    58441 cgcctccacc ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat
    58501 agaaacaccc ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt
    58561 cacgatatta tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac
    58621 cggaaataat aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag
    58681 caaaacgatt gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt
    58741 gatcgcttca gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa
    58801 agagccaccg gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc
    58861 tcccccgcca tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa
    58921 taaaataata acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc
    58981 gactaaaacg acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga
    59041 caataatgcc aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa
    59101 aggcttacct actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat
    59161 tttacttcaa gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact
    59221 gaccttaaaa gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc
    59281 gctttttcaa acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg
    59341 aattctataa taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat
    59401 cttaactttt ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag
    59461 catagcgcag ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa
    59521 taagcccaac caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg
    59581 atgcagagca gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt
    59641 attagctcaa aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact
    59701 caggtcaaaa cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc
    59761 cgcttctttg aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat
    59821 tccaaagaca acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata
    59881 aattcctcct gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt
    59941 gatcatttgg aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat
    60001 cgacagtact aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa
    60061 gacatagacg atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac
    60121 cggattttgg acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg
    60181 cgcaatcgcc attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa
    60241 attgtttcgg tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac
    60301 agcgccgacc cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc
    60361 gattggataa agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt
    60421 tgttgcgtct agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt
    60481 tcggatcgcg tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat
    60541 caagtccgca gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt
    60601 caatgtaccg gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc
    60661 caatcctaat ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt
    60721 gatgccattg acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc
    60781 ttgtccaatt gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac
    60841 agttgctttc gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc
    60901 gccttgtgca gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact
    60961 gatttgacct gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat
    61021 ttgatctagc aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc
    61081 agataggcgg ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat
    61141 tggctgtaat ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt
    61201 atcgattgca ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat
    61261 gtttttcttt tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat
    61321 taacgaacct gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt
    61381 tgtgatgctt ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag
    61441 atcacttgcc tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat
    61501 gatcgtcaag ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc
    61561 aaaatcgccg tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc
    61621 ttgcaaatca aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc
    61681 aacgactttt tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt
    61741 attcagtttt tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta
    61801 gccgtcgatc gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat
    61861 acttactagg ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga
    61921 actggctgtt ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc
    61981 ggtgattttc ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt
    62041 cttgatatca ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat
    62101 acctgcgtaa tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa
    62161 ttgccatcct aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac
    62221 ttctttacct tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc
    62281 agtatttcca tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat
    62341 gatcatgatc gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc
    62401 aagcaaatat aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt
    62461 ttcagctgaa ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa
    62521 gtcgaatttt tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa
    62581 taattaagga ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc
    62641 aaagttttta tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc
    62701 tacaaaaatg tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac
    62761 aggatgattt tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc
    62821 ttaagtgaag cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct
    62881 ttgattatgc cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca
    62941 taaaagcctt cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac
    63001 ctgccgcctg gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca
    63061 tcgattacaa ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca
    63121 gcaaatgctg aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt
    63181 aagattcgga ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat
    63241 gctcctagta tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta
    63301 taagccgtaa ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga
    63361 atttttgcta cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca
    63421 tggacagcta ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca
    63481 ataccagcgc catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt
    63541 tctcctaaac gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga
    63601 acatgtccag aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct
    63661 tctccgtaac cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt
    63721 tacgtttatg cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa
    63781 acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat
    63841 tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga
    63901 ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat
    63961 taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac
    64021 gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga
    64081 tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag
    64141 ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt
    64201 ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac
    64261 gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg
    64321 ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg
    64381 gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt
    64441 atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt
    64501 agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc
    64561 aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg
    64621 tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct
    64681 ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc
    64741 aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt
    64801 tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc
    64861 ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca
    64921 atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc
    64981 cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg
    65041 ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa
    65101 cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca
    65161 tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc
    65221 gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat
    65281 atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata
    65341 cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga
    65401 tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt
    65461 tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat
    65521 catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct
    65581 gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg
    65641 aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg
    65701 cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat
    65761 tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca
    65821 tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat
    65881 gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat
    65941 tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag
    66001 ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa
    66061 aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg
    66121 gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt
    66181 ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa
    66241 ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga
    66301 aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat
    66361 tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt
    66421 tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc
    66481 catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg
    66541 gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg
    66601 cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg
    66661 caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt
    66721 cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt
    66781 agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg
    66841 tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat
    66901 gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta
    66961 aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat
    67021 ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc
    67081 gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca
    67141 tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg
    67201 tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat
    67261 tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt
    67321 gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat
    67381 ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca
    67441 tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc
    67501 caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat
    67561 ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta
    67621 attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc
    67681 ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt
    67741 ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg
    67801 gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga
    67861 tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca
    67921 gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg
    67981 cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca
    68041 tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc
    68101 aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt
    68161 gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac
    68221 tatctgttgc ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa
    68281 aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt
    68341 gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg
    68401 catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc
    68461 gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg
    68521 cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt
    68581 tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga
    68641 agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga
    68701 tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt
    68761 ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg
    68821 ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc
    68881 cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt
    68941 gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg
    69001 tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca
    69061 tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg
    69121 ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc
    69181 ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct
    69241 gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa
    69301 caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat
    69361 gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt
    69421 caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa
    69481 tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt
    69541 ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta
    69601 gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc
    69661 atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc
    69721 ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa
    69781 ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa
    69841 aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta
    69901 ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt
    69961 attattgata tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg
    70021 caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat
    70081 tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag
    70141 ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac
    70201 aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt
    70261 ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat
    70321 atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata
    70381 aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg
    70441 taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc
    70501 tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct
    70561 ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac
    70621 gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat
    70681 ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc
    70741 ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa
    70801 acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt
    70861 tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg
    70921 atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc
    70981 aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc
    71041 tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat
    71101 ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac
    71161 acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata
    71221 ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc
    71281 aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag
    71341 atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa
    71401 actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa
    71461 atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg
    71521 aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg
    71581 gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact
    71641 tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag
    71701 aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat
    71761 ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca
    71821 gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct
    71881 gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga
    71941 cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc
    72001 gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac
    72061 caagaactga caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg
    72121 caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt
    72181 gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat
    72241 cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat
    72301 ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct
    72361 gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga
    72421 aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc
    72481 aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc
    72541 caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct
    72601 attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag
    72661 aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt
    72721 ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact
    72781 tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa
    72841 tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg
    72901 agcataaata aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat
    72961 tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc
    73021 ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt
    73081 gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg
    73141 caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat
    73201 accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg
    73261 attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc
    73321 ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa
    73381 tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta
    73441 aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct atcggcactg
    73501 attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa
    73561 caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat
    73621 acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat
    73681 ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac
    73741 catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg
    73801 gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg
    73861 ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat
    73921 aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat
    73981 ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga
    74041 tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc
    74101 gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg
    74161 tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa
    74221 ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag
    74281 tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt
    74341 actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat
    74401 tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag
    74461 gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga
    74521 tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag
    74581 tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg
    74641 ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg
    74701 ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt
    74761 aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt
    74821 ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt
    74881 tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac
    74941 ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt
    75001 aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt
    75061 cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga
    75121 tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt
    75181 gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt
    75241 cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat
    75301 ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct
    75361 tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt
    75421 tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa
    75481 aaacttccgg ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta
    75541 cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat
    75601 ggaataaaaa gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca
    75661 caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt
    75721 cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt
    75781 ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata agctggacaa
    75841 aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa
    75901 tacaactata taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga
    75961 tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca
    76021 ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc
    76081 catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga
    76141 tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt
    76201 ttcatttatt aaaactgcct cct
//
LOCUS       DAZAJZ010000004        66173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000004 DAZAJZ010000000
VERSION     DAZAJZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.004"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..669
                     /locus_tag="ACG86D_000274"
     CDS             31..669
                     /locus_tag="ACG86D_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN3139455.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(788..860)
                     /locus_tag="ACG86D_000275"
     tRNA            complement(788..860)
                     /locus_tag="ACG86D_000275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:Thr,seq:cgt)
     gene            981..1223
                     /locus_tag="ACG86D_000276"
     CDS             981..1223
                     /locus_tag="ACG86D_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139456.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1362..1991)
                     /gene="upp"
                     /locus_tag="ACG86D_000277"
     CDS             complement(1362..1991)
                     /gene="upp"
                     /locus_tag="ACG86D_000277"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN3139457.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2125..3072)
                     /locus_tag="ACG86D_000278"
     CDS             complement(2125..3072)
                     /locus_tag="ACG86D_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN3139458.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3225..4469)
                     /gene="glyA"
                     /locus_tag="ACG86D_000279"
     CDS             complement(3225..4469)
                     /gene="glyA"
                     /locus_tag="ACG86D_000279"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN3139459.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4660..5685)
                     /locus_tag="ACG86D_000280"
     CDS             complement(4660..5685)
                     /locus_tag="ACG86D_000280"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN3139460.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5707..5886)
                     /locus_tag="ACG86D_000281"
     CDS             complement(5707..5886)
                     /locus_tag="ACG86D_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139461.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5879..6718)
                     /gene="prmC"
                     /locus_tag="ACG86D_000282"
     CDS             complement(5879..6718)
                     /gene="prmC"
                     /locus_tag="ACG86D_000282"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN3139462.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6711..7784)
                     /gene="prfA"
                     /locus_tag="ACG86D_000283"
     CDS             complement(6711..7784)
                     /gene="prfA"
                     /locus_tag="ACG86D_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN3139463.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7787..8371)
                     /locus_tag="ACG86D_000284"
     CDS             complement(7787..8371)
                     /locus_tag="ACG86D_000284"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN3139464.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8477..8725)
                     /locus_tag="ACG86D_000285"
     CDS             complement(8477..8725)
                     /locus_tag="ACG86D_000285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139465.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(8716..8946)
                     /locus_tag="ACG86D_000286"
     CDS             complement(8716..8946)
                     /locus_tag="ACG86D_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139466.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            9052..9654
                     /locus_tag="ACG86D_000287"
     CDS             9052..9654
                     /locus_tag="ACG86D_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN3139467.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9758..10327)
                     /gene="lepB"
                     /locus_tag="ACG86D_000288"
     CDS             complement(9758..10327)
                     /gene="lepB"
                     /locus_tag="ACG86D_000288"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3139468.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10557..11324)
                     /locus_tag="ACG86D_000289"
     CDS             complement(10557..11324)
                     /locus_tag="ACG86D_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN3139469.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(11321..11800)
                     /locus_tag="ACG86D_000290"
     CDS             complement(11321..11800)
                     /locus_tag="ACG86D_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN3139470.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11925..12662)
                     /locus_tag="ACG86D_000291"
     CDS             complement(11925..12662)
                     /locus_tag="ACG86D_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN3139471.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12823..14223
                     /locus_tag="ACG86D_000292"
     CDS             12823..14223
                     /locus_tag="ACG86D_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN3139472.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14446..15798
                     /locus_tag="ACG86D_000293"
     CDS             14446..15798
                     /locus_tag="ACG86D_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN3139473.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15791..16489
                     /locus_tag="ACG86D_000294"
     CDS             15791..16489
                     /locus_tag="ACG86D_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN3139474.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16551..17702)
                     /locus_tag="ACG86D_000295"
     CDS             complement(16551..17702)
                     /locus_tag="ACG86D_000295"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN3139475.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17715..18152)
                     /locus_tag="ACG86D_000296"
     CDS             complement(17715..18152)
                     /locus_tag="ACG86D_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3139476.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18167..19933)
                     /locus_tag="ACG86D_000297"
     CDS             complement(18167..19933)
                     /locus_tag="ACG86D_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN3139477.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20197..20874
                     /locus_tag="ACG86D_000298"
     CDS             20197..20874
                     /locus_tag="ACG86D_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3139478.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20877..22004
                     /locus_tag="ACG86D_000299"
     CDS             20877..22004
                     /locus_tag="ACG86D_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139479.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22204..22644)
                     /locus_tag="ACG86D_000300"
     CDS             complement(22204..22644)
                     /locus_tag="ACG86D_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3139480.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22655..24688)
                     /locus_tag="ACG86D_000301"
     CDS             complement(22655..24688)
                     /locus_tag="ACG86D_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN3139481.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24690..26393)
                     /locus_tag="ACG86D_000302"
     CDS             complement(24690..26393)
                     /locus_tag="ACG86D_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3139482.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26487..27437)
                     /gene="manA"
                     /locus_tag="ACG86D_000303"
     CDS             complement(26487..27437)
                     /gene="manA"
                     /locus_tag="ACG86D_000303"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN3139483.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27476..28501)
                     /locus_tag="ACG86D_000304"
     CDS             complement(27476..28501)
                     /locus_tag="ACG86D_000304"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3139484.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28710..29579)
                     /locus_tag="ACG86D_000305"
     CDS             complement(28710..29579)
                     /locus_tag="ACG86D_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3139485.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(30283..32175)
                     /locus_tag="ACG86D_000306"
     CDS             complement(30283..32175)
                     /locus_tag="ACG86D_000306"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139486.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32172..33899)
                     /locus_tag="ACG86D_000307"
     CDS             complement(32172..33899)
                     /locus_tag="ACG86D_000307"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139487.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33915..34565)
                     /locus_tag="ACG86D_000308"
     CDS             complement(33915..34565)
                     /locus_tag="ACG86D_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN3139488.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34769..35065
                     /locus_tag="ACG86D_000309"
     CDS             34769..35065
                     /locus_tag="ACG86D_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3139489.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35257..35877
                     /locus_tag="ACG86D_000310"
     CDS             35257..35877
                     /locus_tag="ACG86D_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN3139490.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35969..37135)
                     /locus_tag="ACG86D_000311"
     CDS             complement(35969..37135)
                     /locus_tag="ACG86D_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3139491.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37147..37500)
                     /locus_tag="ACG86D_000312"
     CDS             complement(37147..37500)
                     /locus_tag="ACG86D_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN3139492.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37628..38650
                     /locus_tag="ACG86D_000313"
     CDS             37628..38650
                     /locus_tag="ACG86D_000313"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN3139493.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            38669..38830
                     /locus_tag="ACG86D_000314"
     CDS             38669..38830
                     /locus_tag="ACG86D_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139494.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(39089..39523)
                     /locus_tag="ACG86D_000315"
     CDS             complement(39089..39523)
                     /locus_tag="ACG86D_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139495.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39633..41492)
                     /locus_tag="ACG86D_000316"
     CDS             complement(39633..41492)
                     /locus_tag="ACG86D_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3139496.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            41493..41633
                     /locus_tag="ACG86D_000317"
     CDS             41493..41633
                     /locus_tag="ACG86D_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN3139497.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(41610..42770)
                     /locus_tag="ACG86D_000318"
     CDS             complement(41610..42770)
                     /locus_tag="ACG86D_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3139498.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(43077..44501)
                     /gene="araA"
                     /locus_tag="ACG86D_000319"
     CDS             complement(43077..44501)
                     /gene="araA"
                     /locus_tag="ACG86D_000319"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN3139499.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44517..45215)
                     /locus_tag="ACG86D_000320"
     CDS             complement(44517..45215)
                     /locus_tag="ACG86D_000320"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN3139500.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45172..46833)
                     /locus_tag="ACG86D_000321"
     CDS             complement(45172..46833)
                     /locus_tag="ACG86D_000321"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN3139501.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47051..48451
                     /locus_tag="ACG86D_000322"
     CDS             47051..48451
                     /locus_tag="ACG86D_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN3139502.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48703..49782)
                     /locus_tag="ACG86D_000323"
     CDS             complement(48703..49782)
                     /locus_tag="ACG86D_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3139503.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49868..50827)
                     /locus_tag="ACG86D_000324"
     CDS             complement(49868..50827)
                     /locus_tag="ACG86D_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN3139504.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50839..52350)
                     /locus_tag="ACG86D_000325"
     CDS             complement(50839..52350)
                     /locus_tag="ACG86D_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN3139505.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52372..52935)
                     /locus_tag="ACG86D_000326"
     CDS             complement(52372..52935)
                     /locus_tag="ACG86D_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3139506.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(52941..53804)
                     /locus_tag="ACG86D_000327"
     CDS             complement(52941..53804)
                     /locus_tag="ACG86D_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3139507.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53818..54714)
                     /locus_tag="ACG86D_000328"
     CDS             complement(53818..54714)
                     /locus_tag="ACG86D_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3139508.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54889..56178)
                     /locus_tag="ACG86D_000329"
     CDS             complement(54889..56178)
                     /locus_tag="ACG86D_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3139509.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56150..57667)
                     /locus_tag="ACG86D_000330"
     CDS             complement(56150..57667)
                     /locus_tag="ACG86D_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN3139510.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57865..58755
                     /locus_tag="ACG86D_000331"
     CDS             57865..58755
                     /locus_tag="ACG86D_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN3139511.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58828..59652)
                     /gene="nadE"
                     /locus_tag="ACG86D_000332"
     CDS             complement(58828..59652)
                     /gene="nadE"
                     /locus_tag="ACG86D_000332"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN3139512.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59664..61136)
                     /locus_tag="ACG86D_000333"
     CDS             complement(59664..61136)
                     /locus_tag="ACG86D_000333"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN3139513.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61773..62135)
                     /locus_tag="ACG86D_000334"
     CDS             complement(61773..62135)
                     /locus_tag="ACG86D_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN3139514.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62358..63110
                     /locus_tag="ACG86D_000335"
     CDS             62358..63110
                     /locus_tag="ACG86D_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3139515.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63250..64173)
                     /locus_tag="ACG86D_000336"
     CDS             complement(63250..64173)
                     /locus_tag="ACG86D_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3139516.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64188..64460)
                     /locus_tag="ACG86D_000337"
     CDS             complement(64188..64460)
                     /locus_tag="ACG86D_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139517.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(64804..65991)
                     /locus_tag="ACG86D_000338"
     CDS             complement(64804..65991)
                     /locus_tag="ACG86D_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN3139518.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT        19083 a        13625 c        11536 g        21929 t
ORIGIN      
        1 ttcaaaaact gaatagtagg agttattatc atgaataaca ttttaggaaa aaaaatagaa
       61 cttaaaaaag ggatattggg cactattgtc gttgtactcg gtttgttagg gttgctattt
      121 gcgccatcca ttattagcat tttttcttta actatcactg gatttatagc atttacactt
      181 gtttttggta aaaaagatac gaaaaaaatg ttctcgaaac ctgtcgctcc tgtcaaaaat
      241 atagccaaat atttttttat caatgtagta atcagtgccg cagtctcggt atttttgcaa
      301 caaattttga aatggggttt aacaggcaat ccaataaatg aggctttcag cccgttatta
      361 tttattattt tgcctatcat gatacttgga gaagaattgt tctcagtgta ttttctcgct
      421 attttttcta gtaagttcag cattcctgtc gctagtttct tgtcagcaat catctttgga
      481 tttatccatt acagtactta tgataatgga aatattttac acactgtagc acacatcctt
      541 ttgatacaag gggtggcaag attgttattc aaccaagctg ccattaaaag caactcgatc
      601 attacaagtt gggcagttca tgtgatattt gatttcacag ctatattgct agtcgtttta
      661 ttctcctaaa tttttataga aataatacat gctctattgc taagaaagca tactttattt
      721 cctttaaaca aaaagacaga tgtatcaatg gattaaaaat ccttgatata tctgtcttct
      781 cttttaatgc cagctatagg gatcgaacct acgacctacg cgttacgagt gcgttgctct
      841 accaactgag ctaagctggc cttatccaaa agaaattata tactctattt ggctgattgt
      901 aaagagtccg atggaaaatt agtttaattt catgatattt ttcataaaac gtggtataat
      961 tgatgtgaaa ggcaggtttt atgaaaatgg caacttttgg aaaatttgat acaactatcg
     1021 taccatcgga aattggtgaa aaattcaaga tcaatactca cgtaatgttc acaattcacg
     1081 gcaagtcgca aactggaacg atttcaaagc aattgaaaaa ttccgctgtt gttgagattg
     1141 atgaaactgc agaaaatgcg aacttgatga cgcaaagtaa gggtgtagta atcgttaact
     1201 acaaagaact aaaaaagatt tgaatttgac catctctgca tttttgggat ggttttttta
     1261 tatgcaaacg aaagctgtga aaaagctgtt ctgcatcaaa cgttaaaaat gaccaaaaaa
     1321 ggatgtgaca aaagttgtgt cacacccttt ttcgtattta cttattttgt accaaacaaa
     1381 cggtcacccg catcccctag tccaggaacg atataaccat gttcattcaa tttttcatct
     1441 agagaagctg tatagatgtc aatatcagga tgcgcttctt gcaaagcttt tactccctca
     1501 ggtgctgcta ccaaacaaac aaatttaatg ttcgacgcgc cacgttcttt caatgcatcg
     1561 attgccatga ttgctgatcc gcctgttgcc aacattggat ctactacaaa caactgacgt
     1621 gcatcaatgt cttctggaag tttgacgaaa tattcatgtg gttccaatgt ttcttcatct
     1681 cgataaagac cgatatgtcc cactttagca gctggaatca attccaagat accgtcaacc
     1741 attccaatac ctgcgcgcaa gattggaata atcgctactt ttttaccaga caacgttttt
     1801 tgtgttgttt cagtgatcgg tgtttcgatc acgatgtctt ccaatggcat atcccgtgat
     1861 acttcatatg ccatcaacat agcgatttca tcgactactt cacgaaaaac ctttgttccg
     1921 cagtttttgt cccgaataat agttagtttg tgttgaatca atggatgatc gatcacttga
     1981 aatttgccca ttttttaagc tctcctttgt tctgtcgttt ctttttaatt gtaaaacaaa
     2041 atcaagtgat tgactagtca aactcgccat ttttcctaag aaaatccgtt tttgaaaaga
     2101 agttttcctg cggacaagca aagcttattc ttttttcttg atccaagaag tagataatgc
     2161 cattgctgca cgcgttgcct ttgtctgatc catagcattt accatgacaa aatcatggat
     2221 gattccttgg aagcgtactt gtgtaacagg aacccctgca tcacgaagtt tgcgcgcata
     2281 agcctcacct tcatctctta atacatccgc ttctcccgtt aaaatcattg ctgaaggaag
     2341 atcatttagt tcatttatat ttgctctcaa aggcgaagct gtgatttcag cacgctcttc
     2401 ttcattcgtc gtatattggt cccaaaacca catcattcct tctcttgtca aaaaatagtt
     2461 ttctccgaat tcatgatagg aatctgtatc aaaatcagcg tttgtgactg gataatacag
     2521 caattgcttg gatattggga ggccctttcg ttcttttgtt aagatcgtca tgactgtagc
     2581 catgttacca cctactgaat ctcctgcaac gattagattc tcgagatcca gattttttga
     2641 cttggctaat ttattcagct gttgcaacac agcataattt tgttcaattg ctgttggata
     2701 ttttgcttct ggtgatcgac tatattctgg aaagacaacg acggaattcg tacgaaccgc
     2761 aagttctctg atcaatttat catgggtttg agcactgcca aaaacccagc ctgctccatg
     2821 aatataaaaa ataactggta atttatccat attcccttct ggtcgtacga gacgaatatt
     2881 gagttttccc cattcgccag tatccaccat caagtcttca atatcgacgg aatatttgtt
     2941 cacaggtgat tcttgtactt cttctagtaa aactcttcct tcttccggtg ctagttcata
     3001 gatacgcgga tgaggcgcat ttgcatcact gaatgctact gcttcctttt ctaacgagat
     3061 acgatcagtc atgacttttc ctccctgaga tttagtgtga agactaaaat tacacctcat
     3121 atactagaga gcatcaccga tattgtcaaa gaatccgttt tcccaaaaaa taaacagaaa
     3181 agcccaacat tgaacttttc tgtttactaa tggaatttaa atttttattt tttatgcaac
     3241 ggaaattttt cagttaattc gcgtacacct gttttgactt cgtctagttg agcgttatca
     3301 tcttttgctt gtaatgcttt gacaactagt tcagccactt ttacagcatc ttcttctttg
     3361 aacccgcgag tagtgattgc tggcgtgccg attcgaatac cgcttgtttt gaatggactt
     3421 agtgtttcaa aaggaatcga gtttttattt acagtgatat tcacactgtc caaaatgctc
     3481 tctgcttctt ttccatttat acctaactcg cgaacatcga tcaacatcag atgattatct
     3541 gtcgcccctg aaatgactct ggttccaatt gcttgattga aaactttgac cattgctttt
     3601 gcattggcga tgatttgttc actatattct ttaaatgcag gatctaatgc ttctttgaat
     3661 gctactgctt tacccgcaat cacatgctct agagggccgc cttgaatacc aggaaatacc
     3721 gcactattta ttttttttgc taatgcttca tcgtttgtca aaatcatgcc gcctcgaggg
     3781 ccacgcaatg ttttatgagt ggtagtcgtt gtgatatctg cataaggaac tggattagga
     3841 tgaagtcctg ctgctactaa tcctgcgata tgtgccatat ctaccatcag ttttgcgcct
     3901 acttcatctg cgatttctct gaatttcgca aagtcgatcg ttcgtccgta agcactggca
     3961 cctgctacga tcaattttgg ttgatgtttt ctagctaaaa tacgtaccac attataatca
     4021 ataacttctg ttgtcggatc aacaccgtaa gcaacaaagt gataggtttt gccactgaag
     4081 ttgactggtg atccatgtgt tagatgtccg cctgcggaaa gatccattcc taagatcgtg
     4141 tcccctggtt ctaccaaagc gagataagca gccgtgttgg cttgagagcc ggagtgaggc
     4201 tggacgttcg caaatttcgc tccgaataat tcttttgcac ggtcaatcgc taaattttca
     4261 acgatatcga caaattcaca cccaccataa tagcgatgcc ctggataacc ttctgcgtat
     4321 ttatttgtca agatacttcc ttgtgcagcc atcactgctt cggagacaaa gttttcagag
     4381 gcaatcagct ccaaattatg ttcttgtctt tcttcttctt tggcgatcgc agcccaaaga
     4441 tcagggtcaa acgttttgta atctaccaca aacaacagct cctttttatt ttcctacaat
     4501 tttattttcc tacaatttta tttttcccat caaaaacaaa ttgccaaata ttcgtatctc
     4561 ttctattacg attagttacg cttagttttt cgttcactga tagaagattt acttgatagc
     4621 attgtatcat actaaaagaa tgcgacaata accaattgat catttttcaa aataattttg
     4681 tcctgctgct tttttcaagc gattcatgta agcattcccc aatccagttt ctggatacac
     4741 ttgtacaaaa atcaaatcta atccaagtgt tggttcatct aatcctctta gacctgcaaa
     4801 caatccttta gccgccgctt ctactgagtt gtcgttatac atataaacag ctgcagtatc
     4861 tgtccgcact tgatccgcaa tttcggggct tgctatgaca cctgcacgga tcttcttatt
     4921 ttttgcccat tgaactgcag ttgaccaatc gccatctctg accatcagaa cacgtgtatc
     4981 gggtgagtag tgtttgtatt tcatcccagg ggcttttggt gtttcatttt cttttacaag
     5041 atgctgatca atagcaacag gagattcgat gactgcttcg atttgttctt tcgttactgc
     5101 acctggtcgt aaaatcatag gcatggctgt tggatcactt aaatcaagaa cagtggattc
     5161 tacaccaatt cgcgtagctc catcatcaat aatcccggta atttttcctt gtagatcatg
     5221 atatacatga tctgcagtcg ttggacttgg ttttcccgac gtatttgcac ttggaccgac
     5281 aagcggaaca ccggaaagtt cgattacttc tagtgttttt ttgttatccg gtatacgaaa
     5341 tgcagctgtg gataatcctc ccgtcacaac tgagggcaaa gtgtcttttt tgatcttaaa
     5401 tatcaaagtt aatggtccag gccagaagtt cttcacaatc ttctctgctg ccggatgaaa
     5461 gttgtccaca tactctttta cttgttcaaa agaggcgaca tggacgatta atggattatc
     5521 ttgcggtctc ccttttacag aaaacacttt cttgacagcg tttggtaaaa gagcgttggc
     5581 gcccaatccg tatactgttt cggtaggaaa agcaacaagt tctccttctt tcagtgcttc
     5641 ggctgcctct tgcaattctg tttcatcata tcgtttcgtt tccacagttt atccacaacc
     5701 cctttttcat acattcttat tttgtctgag tttatcctca agttttccac tgtttgtgga
     5761 taatctattt gtcaatagtg gataaacttg ttgataaacc aaaaagtcgt ttacatcctt
     5821 gtcaaataag tgttttttta taataaaaaa acaaataaaa aatttttcat ccacaacttc
     5881 aaccataaac ttggatcatt cgttcattgc caaaaagatc cttttttatc gccacttttt
     5941 tatctggaaa tgcttgctga aatatttttt ttactgcttc accttggcga aatccgattt
     6001 ctaaaaaaat ctttccattt gattttagta cgcttggtgc ttcaactgct attttttcat
     6061 aaatagctaa tccatcgttc tcagcaaaaa gggccatttt aggttcgaat gaccggacac
     6121 tttcatccat cagttcccat tcattccgac tgatgtacgg cggattagaa ataatcacat
     6181 caaattgctg atccattact ggtcctaatg tatctccatg ataaaaactg attttcgttc
     6241 cttctttctg agcattttct ttcgctactt ctaatgcttc ttctgaaaga tccacagcag
     6301 atatatgcca attttttctt gctgctttca aactaatagc aattgctcca gtccctgtcc
     6361 caatatctat cacttctaac ggtacaccag gcgtctcgtc taaacaccat tcgactaact
     6421 cctctgtttc tggtcgaggg atcagtgttg cttcagttac ctttaaccga tgatcataaa
     6481 aatctgcata tcctagcaaa tactgaggtg gatgatctgc aagcagaagt cgcagatcgg
     6541 tttcgatcaa ccgctgttct tcttctgaaa tctcttcgtt catatgaagg agccaatcaa
     6601 gttttttcca ttgttttcgt tccaaaaata aaaattggat actatatgct tctttcccat
     6661 gatcctctaa aaaagaagaa gcccgtgaca ggacttctcg ataggtcatt ttacccattt
     6721 tgcatagctt ccagttttga tgtttgatca tacatgatca atgcatcaac gatctcatct
     6781 aatttccctg ctaaaatctg atctaatttt tggatagtta atccgatacg atgatcagtc
     6841 acacgatttt gcgggaaatt atacgtccgg atccgttctg aacgatcccc agttccgact
     6901 gctgattttc gattggcatc atactcgctt tgtgcttctt gctggatctt atcgtacaca
     6961 cgagcacgca agattttcat tgctttttca cggttcttga tttgagaacg ttcatcttgc
     7021 atagcaacaa cgatccctgt tggtaagtga gtcaaacgta cagcagatgc tgttttattg
     7081 acgtgctgtc cgccagcacc tgaagcatga tagatatctg tacggatatc tttgtcttca
     7141 atatcgatct ctacttcttc agcttctggc atcacgacaa ctgttgcggt ggaagtatgg
     7201 atacgccctt gcgattcagt tgaaggaaca cgctggacgc ggtgcgcacc actttcatat
     7261 tttaatttcg aatatacgtt gtctccggtg atcatcatga tcacttcttt atatccaccg
     7321 attccggtaa cgttagcttc catcacttct gttttccagc cttgtgcttc agcatacttt
     7381 tggtacatat tgaaaagatc gccagcgaat aatgctgctt cgtctccgcc tgctgctcca
     7441 cggatttcca tgatgatatt tttatcgtca tttggatctt ttggtaaaag caagatcttg
     7501 attttttctt ctaagacttc tttttctttt tttaattcag ataattcttc ttttgctagt
     7561 tcagccattt catcatccag tttttcacca agcagttctt ctgtatcggt gattccttgt
     7621 actacttctt tataacgacg ataaacttcg actgtttcgc gcgtgtttgc ttcttctttt
     7681 gaaagctgca taaaacgctt tgtatcagaa atcacttctg ggtcacttaa aagttctcca
     7741 agctcttcat aacggtcttc aattgcttgt aattgatcgt acatgcttat tctccttctg
     7801 taatcattga tgggtgaaag taatgttttc gacaaacggg gtaataagct tcatttccgc
     7861 caatttgcac ttgatcgcct tcataaacag ggtttccatc aatataatgt aaattcatga
     7921 ttgctttttt gtggcagaac caacagattg ttttcatttc ttcgattttg tctgcataaa
     7981 gcaataaata ttttgatcct tcaaataact cgtttcgaaa atcatttttc aaaccaaatg
     8041 ccataaccgg aatatccaat tcgtccacaa ttctcgtaaa agcaatgaca tgtttctttt
     8101 ttaagaattg cgcttcatcg atcagcacac aatagggttt gctttccatt ttctgaatta
     8161 cgtcaaatac gtctgtttct tcaaatatcg gcatcgcatt acgtttcagt ccgattcggc
     8221 ttgaaacaac gcctaaacca tctcttgtat caataccgct tgtcattaag acaactggct
     8281 tattttgttc ttcataatta tgtgcgactt ttaggatctc gatcgtcttg ccgctattca
     8341 ttgcgccata cttaaaaaat agttgtgcca tgctcttttt caccttccag tttgttccta
     8401 tggtattata cttgattttc agcatttttt acagtgagaa ttttcatcaa gcacagatta
     8461 gtattaaatc tattgctcac ttttgttcca attttttgac aagaactaaa catgggttcc
     8521 attcatccca taaatttgga aaaacttcca accttgaaaa tcctaatgat tcgtagaaac
     8581 aaatagtttg atcatatata gagtagtgcc cctcgtctac agtcttcact tgcaaatatt
     8641 tgtaatgttt tgctgcgtaa ccttctgctt ctttaaaaag ttttgttcct aatccttggt
     8701 gatgatagtc tttttttatt cccatacaat agaatagatt tcggcagtat cttcacttgt
     8761 ctgtgaaaga gtgataaagc ctaaatattc ttcttttctt ttttcacacc ataaaggtag
     8821 tttacttgat tcctcgatat atttttccgt tgattcagga agcccaaacc actcaggcaa
     8881 gtctagtaat atttctcgga cacactttcc tttttcctct ctatctttta caaacgatat
     8941 cggcatattc tttctagatt ctcccttttt gaattgagta tagcatgctt tctttctttt
     9001 cacgtgctat aataagttaa aatttcaaag ttttacgtaa ggagctattt gatgacaaaa
     9061 aatatttttg attccatcgc taaccattat gattcccctg accgtcaagc attagccaag
     9121 attattcgcg aagaactaac tacttatcta ccgcgcgaca gccaccaaaa agtcctgctg
     9181 gattacggag gcggaactgg gcttgttagt ttaccgcttg cggaaagatt caaagaactg
     9241 atcattgccg atgcttcaga aactatgctg aaaatggcag aagaaaagat ccaagcagca
     9301 gatctgaaaa atgtccgaac gatccacgct gatgcttctg tcgaatttcc tgctgtccaa
     9361 gctaatctca tccttctatc acttgtactt ttacacattc ctgatacgga gaatattctt
     9421 acaaaacttt atgaaatttt agctcctggc ggtcaattga tcatcgtaga ctttgacaag
     9481 aatgaacaaa ttagtcatcc gaaagtccat aatggcttta gccaagaaga attgaataat
     9541 cgattggaaa aaacgggctt tgtctctgct actagtcata ccttccatcg tggggaaaag
     9601 ctatttatga acaaaaacgc ttcgcttttc ttatccatca gccaaaaagc ttaaaatgag
     9661 attgcaaaaa aagtgagcaa ctccaagaaa taagaaggaa ttttcggaaa aagaagctgt
     9721 gacaaaaaac atgtcacagc ttctttttat tttttcatca catgatttta atttctgaaa
     9781 gaggccaata acggaaaaca acttttcctt ggatcatatc tttgtcgact aacccaaaca
     9841 ttcggctgtc ttttgaaatc aagcggttat cgcctagaac aagatacttt ccttttggca
     9901 cagtatattc aaagtcactc gtaaaggtgg atgaactttc tgcttgtgct tggaatcctg
     9961 agctataagc atattctcct tgtaacttat cttctgaaaa ttcttttttg aactcatcca
    10021 gataaggttc atcatacttt ttgccattaa tggtcaactg gtcattttcc atcttgactg
    10081 tatctccagg cataccgatg atccgtttaa caatcatccg ttcttcatcc cccggttcct
    10141 ttgtagtaat aatatccatt cgttcatagt ttgagatctt agatgaaatc aatcgttgtc
    10201 catcacttaa tgtagggtcc atcgaatgcc cgtttacagt aactggtgta aacacgtaca
    10261 ctctagctaa taaaatagcc gctaatacaa ggatcaaaat cccccaattt ttcaaaaatt
    10321 ctttcacagc ttacctccta cttgtatttt cctcgttatt tattacgttt tttcacaata
    10381 cttcgttgta ctttctttat tctacaatgt ttataaatga aaggaaagaa taagtgatgt
    10441 gttcgtctta tctttctcgt tatataaaaa aggaacgtga caaaagacgt gtcacatccc
    10501 ccttattccg aataaacgtt tttatcgcaa ccactgatac acggcgtttc atcacattat
    10561 gaatgtcttc ttctttggct acgactataa accactggtt cttcctgcga ctgtgccttt
    10621 tttgcaatca atttcagacg ataaggctca tgatagcgta acccgtcttt gacaaaatca
    10681 ccgccgacga tgaacaatcc gacaggttcc aatgtgtctt ctgttgagtc aagtcgaatg
    10741 gtccctactt tttccatgtc cgagtagctt cgtccaatat ttagaacata ctctttctct
    10801 cctgttttct tgacatagta ataaggatac aatgtatttc tatcgaccag aaatccatcc
    10861 atgcgaccga gttcatttgt tttgatctca ccatttccaa tcaaataccg catattctcg
    10921 gctaatttca attgataatc ttcgtcataa gaaaccatga cctcttgtgt attcacttta
    10981 tcgatcgaat ttccattatc tatcgattct tctgtctgga tgttgaacgc ttcatcaggt
    11041 aaatacttca caaaaagctg agtgacatcg gctttcgtgt ttttatctaa aaagtaataa
    11101 aatacattta aaacagtaaa ataaacaagc actagaaatg caagtccata taagaaaaat
    11161 tgaagatagt tttggttttg ccaaagtcca taaacaattc tcaataaata aagcgtggga
    11221 atcacactta aaatcgtata tccacggtta agatatttag gactgatgtc taaatagcca
    11281 aggaatttat gcaccccgtt gataaggtct agtaaaaatg tcattgagaa tcacctgctt
    11341 gctggttata cgtttgggta tttcctgaat cttggttttg ctgtgctgta cgtgctgcat
    11401 catcattttg cgtgttatca tctacagtcg tactgcttgg tatcgtttcg gagctagcac
    11461 ttgtatccgg acttgtactt ggattggttg aatccgatgg agtgccatct ggtaccgtat
    11521 tacttgtttc gcttgagctg tcagttgtat atgtctcatc atacgacgaa ctagttgttg
    11581 tatcgtccca tgtacttgtc gtagtggttt cattcaaact ttccgttgtt gttggttcag
    11641 atgtcgagac tgaagtagtt ggtgttgttt cttctacagt actagaactt gaacttggtg
    11701 cacggtagct ggaactattc acaatacctg tagtagtcgc gacaatcgtc gagattgtta
    11761 atacagcgat tggcttcaaa ataggtgcta cttttcgcat cttatctctt ccttcattga
    11821 aatttattct tgtttcctta ctttactacg gacaaattat acagtctacc ttatttctac
    11881 cataaattct ttattttttt cttacgtttt tccgaaagat ttctttaatt ttcctcaaaa
    11941 actacggttg atgtttttaa gtaagctttt gcttcccgat tgatttcttt cagatcgcat
    12001 ccttgctgct tggcagcgaa cacgcaacca caataacatt ggcgataaat atcatactct
    12061 ttgcacattt gcaaagagcg ttcatagcct ttgtttttct tgaaatcact tggcaagtat
    12121 tgtgtcccgt aaatcttttg gatatccatt ccgatctgat tgatcaaagc tgcatttttc
    12181 tttggcgaga tggtcagggc acttccaaag taatcgtatc ctagttcttg cgctttttgc
    12241 gcaacaagat ctaatcgcat attgaagcat gcactgcagc gagcaccacc ttctttttcg
    12301 ttttcaagat ggtttgttaa aaccattttg gtgaattcat ttggacggta ttcatcaacg
    12361 attagcgcaa catgatggtc agttttctca ttaaattggt ctacaaatcg tttttgttca
    12421 tgtaatcttc gcaaatactc acttttagga tgaatattcg aatttgaaaa aaataacgtg
    12481 atctctgcat attgagacat gaattccaat gtgtgcgtac tgcaaggagc acagcatgta
    12541 tgtattaata tcttaggctt tacttcttct ttctcccatt gcatgatcat ttttttcaga
    12601 actgtatcat aatttatttt ttgatttttc attttttcta cgatttgttg agcattaatc
    12661 aatttgttat tcctcctcct tcttttttct aggattttcc ttattataag ccatttctat
    12721 tttaatcgaa agctgtattg ttatctgttt gataatttct ttgagtattc ctaattcccc
    12781 ttgtatactg gtagaaacga ctattgtcag gaggatagta ctgtgcatgt tgatttattc
    12841 tttcgaaggc tgcagcggcg aggacagaag tttgctgcca atcatttctc atcgatccaa
    12901 atcatcgttt tttattatat attgatgacg gtcttatcac tcgttctatt ttatatgccg
    12961 atttttcgcg aaccagatag ccatgtttct tttgtggaca tgctgttcat ggcaatcagt
    13021 acagtgagtg tgactggact atcgactttt gatatcaatt ctgtgttcaa tgataacggt
    13081 attatactgc tggaaatatt atttcaagtt ggtggtttgg ggatcatgat gatttctact
    13141 gcttttgtca ttttttctaa acgacggatc acactgaaac agcgacagct gatcatgacg
    13201 gatatgaatc aaccgcgttt gtctggtatc gtccgactga ttcggatcac ttttgctatt
    13261 ttaatctggt ttcaattact ttttgggaca ttcttttcta tttattttta ttacagaggc
    13321 tattttgatc gttggcgtga tgccgttttt tacggttttt atcaggcgat atcggctgtc
    13381 acgaactctg ggtttgacgt cactggggat tccatcaagc cctttgcaca tgactatttt
    13441 ttcttgatcc tgatcatgtt tttgattttt gtcggaggaa ttggttttcc tgtattgatg
    13501 gagtgccggg aatggctatt atacaaacgt tccaatgcga agcttccgtt tcgtttttcc
    13561 ttgtttacca aattagctgt gttagctttt gttatcttgt ttgtttcggg aactgtcttg
    13621 atttatctat tggaaaagga ccatttattc caagattcca atatgagtgt caaatggatc
    13681 aattcgatgt tttattcgat tacgactcgt aatgctggtc tgcagattca cgatttgggt
    13741 gattttcaaa tcacaacact gatcattttt tctttactga tgtttatcgg atgcagtcct
    13801 agttctgtag gcggcgggat tcgaacaact actgtagcaa tcatcggttt gtatctatat
    13861 tccttcctca aaagtgaaga taacatcaat atttttggac ggagaatcga tcaagatgat
    13921 gtgcgaaaat cagtcgttgt ctttatgctt tctcttggta tgtgtttctt ttgtatcgtt
    13981 tttttatctg ctacagaaga acaaacgctg atctcgatca tcatcgaagt aacttctgcg
    14041 tttggaacaa ctggtttatc tttagggatt accggagact tgtctgttgt gggcaaaatc
    14101 acgattgcta cactgatgtt tattgggcgg atcggaatgc tatatacatt gatgttattc
    14161 gtgccaaaag aaacacgaga tctaggctat gaatatccaa gcgagaaaat tattatcggt
    14221 taaaatggga atataggtaa gagggaataa taaaatcaca gcagcttgaa acaatcattt
    14281 tgtttcaagc tgtttttttg ttaaatcatg cgattttgtc cgatttcatg cactttcagc
    14341 cgtaaccttt tcttaagaat aagaaaagca atttaacctt tttttcaaca tatggtatga
    14401 ttagttgaat ttattcgtca gcaaatcgtt tttggaggtt acataatggg aatacgcagt
    14461 cattttgcga ttgcagttgg aaaaacgtcg caatgggcat taaaaacttt ttttaaaggc
    14521 ggatcaagtt taccaggtaa actagcgctg aaagtcgatc cacatatact agatagtttg
    14581 gcaaaagact accaagtcgt cgttgtcacc ggaacaaacg gtaaaacatt gactaccgct
    14641 ttaacagtca atattttgcg ccagcaattc gatgaagttc taaccaaccc tactggtgcg
    14701 aatatggtac agggaattgt atcgactttc ttgcaggcaa aaccaaaaaa aggacagaaa
    14761 aagtttgctg tcctagaaat agatgaagca agtttgagca aagtcacaga atatatgaaa
    14821 ccagaactgt tcttatttac aaatattttc cgtgatcaaa tggatcggta tggagaaatc
    14881 tatacgacct ataaaatgat tgtagacggt gccgcaaagt caccaaatgc aacgattatc
    14941 agtaacggtg attcaccaat cttcaattct atcgaaacag tcaatcctag aaaatattat
    15001 ggctttgacc atgaagaaga tcgtgaacaa atggctcatt acaatacaga cggtgtctta
    15061 tgtccaaatt gtcatcacat cttgcattat aaaatgatca cttatgcaaa cttagggaaa
    15121 tattactgtc caaattgtga cttcaaacgt cctgaattgg attatcgatt aactgatatg
    15181 gtacggatgg acaatgtgtc agcagatttc gtgatcgatg gtcatgaata cggaatcgaa
    15241 gttggcggta tgtataatgt ctacaatgct ttagcagcta ctgcagtggc agagtacttc
    15301 aatgtcgatc ctgataaaat caaacaaggt ttaggatatg acgagaaagt attcggccga
    15361 caagaagtca tcaatatcaa tgggaaaaaa tgtacattag tacttgtcaa aaatcctgta
    15421 ggtatcaatc aagtaatcga tatgattggt ctagctcctt atccattttc tttagtggct
    15481 ttattaaatg ccaattacgc agacggaatc gatgtcagct ggatttggga tggtaatttt
    15541 gaaaactttg ccgatatgga tatcccagct gttatttctg gaggagaccg tcatacagat
    15601 atgactttac gtctaaaagt tgccgggatt ccagaaaata aattgacaga aacaaaagaa
    15661 ttaccagaag taatcgaaaa aatcaaagaa ttgccaactg agcatgtcta tattttagct
    15721 acttacactg ctgtattgca gcttcgtaaa gaactagctg ctcaaggatt tatcaaagga
    15781 ggaatgaacc gtggctaatt atgaactcaa tatcgcccac ctttatggaa atttgatgaa
    15841 cacatatggt gataacggaa accttttgat gttgaaatat atagcagaaa aaatgggggt
    15901 ttcttgccat acggagatcg ttagtataca tgaacctttt gatgctgata aatatgactt
    15961 ggttttcttt ggcggtggtc aagactttga gcaattaatc atttcaaaag acatccagaa
    16021 aaagaaagat tctttgatca actatatcga aaatgatggc gtcatgttag ccatttgtgg
    16081 tggctaccaa ttgctaggtc actactacat gggagctaat ggagaaaaga tccaaggaat
    16141 cagtgcactg gatcactaca cgttaagtca ggaaaataac cggtttatcg gtgatatcgt
    16201 tatccataac gaagaattca atgaaactta ttatggattt gaaaaccaca atggtcggac
    16261 tttcttaggt gaaggtgaac gacctttggg gaaagtggta caaggtcaag gaaataatgg
    16321 tgaagaccaa tctgaaggtg tcatttaccg aaatgtcttc ggttcttatt tccatggacc
    16381 tatccttgca agaaatgaga acctggcagc tcgtttgatc cgcctagctt tagaacaacg
    16441 ttacggtaaa gaattggatc tgccctatct tgaaggacaa acagcctagt tattcgaaaa
    16501 aggggaatgt gacaaaaatt tcgtcacatt cccctttttc gtatcctatt ttaagcgact
    16561 tgcttggcat aacggttcac gttttgcttg atattcccga ttgttttcgg atcattgatt
    16621 cctgtgactt cttgaatcaa ttgttccaag ttttcatgga tcttcatttc atgcaattga
    16681 cggctttgtt catcttcagg atcatagtaa ttaaaaataa tccccattgt tgccgtcaaa
    16741 tgtgggcaag tcaagttatg ttcttgtagt tctctcactg gacgtgtaaa tctttcttga
    16801 taacctaact tacgtaatgg tgtccgagcg acacgtgtga tcgcatcaga gatatttttg
    16861 ttttggaagc gatgaatgat tttttcaata tacgcatgat gctgctctgc atcaaacccc
    16921 catttggcaa tcagcaaact tcctgtttcc tgcaaaactg attttaattg tgccacaact
    16981 aacgcatctt gcattgcttc atcaatggtc tggtaaccta acaaggcacc tgtataagcg
    17041 acagtcgcat gaccagtatt gacactaaat agtttgcgtt cgatatatgg ttctagatca
    17101 tctacatata aaaccccttt taattggatt tttttatttt ttctttggct atcgtcaatg
    17161 acccattctt tgaaaggttc cacttggacg aataaaggat cttcatggtg ttgtaatggg
    17221 acgattcgat cgactgctgc atttggaaag ccgatatatt tgtcagtaaa tgatgtttcc
    17281 ggaagatatt tatacacttc tttttctaga aatgctgaac cgccaatcat attttcacag
    17341 gcaatgatat ctaaagatgt tgtgcttcct tccttttctc tttcttggat accttttgca
    17401 atcaactcag caataaaagg taagatattc gggccaatgg cggtagtaac taaatctgct
    17461 tcagcgattt cttggattac tttttctgga tttgtttgat tgttgattcc tttgacattt
    17521 tttacagtga ttttttcttt tgattcatct gctaattcga tttcgtattc ttttctttca
    17581 tttaaagcat caatgatcgt gtgattcaca tctacgaaag taatctcaaa tccattagct
    17641 gccaaaacct ctccaataaa tcctcgaccg atatttcctg caccaaaatg tactgctctc
    17701 atttttctct ctccttaagc aattgctaga ttttccgtta cttcttcttt gctgagcgcg
    17761 tctgctagct ggacaacatt gtccatatca ctgcaataaa gtgcgatttg ctgtacaagt
    17821 tgcaaatgct catctccgac tcctgcaata ccgaacaaaa cagtcgcaat cttttcttct
    17881 tcttcggtac caaaatctac accatctggt acttgaacga cacagatgcc ggattttttc
    17941 acatattttt ttgcttcatc tgttccatga ggaatcgcga taaaattccc catataaaca
    18001 gataacattt tgtctctatc taccatggca tcgatatatt cctcatcaac gcaccctgct
    18061 tctacaagca tctgaccgca atagcggatc gcttcttctt tggttttaaa agatcgattg
    18121 agctcgatca tgtcgaatgg caaactttgc atgctgtttt cctcccttaa gctgtctgtt
    18181 tcaaatattg tgcaatcgat tcgtattttt gcggattgac catgctgttg actgataaat
    18241 gaatggattg aggtgcagcc atctgtactt tttccgctaa actttctttg gctacgatca
    18301 gtgaatcagg ttcatctttt aaatcttgga tattgacttt tcttaacggg atagttaaat
    18361 ggttcttgtc taatatatct ttgagcacgg tcatccccat tgttgctgat ccggaacgat
    18421 catcgtggac aagtagaatc tctttgattt gtttgatgtt tgctgaaaga aactcttctg
    18481 ctggctcatt ctcaatttca gaaactgatg ttttctgctt gctgcttaat tcttgaatga
    18541 tttcatcata tcgaggtgaa ttcaaaaagt tttctactga gacaaaacgc gcgctcggtg
    18601 ctttttgttt tgctctttct tgtagttcga cctgagtaac gatcaatgta ttcgagtcat
    18661 ctgtcagttg gctgattgct tgattggtca ccgtttgagg caagtctgct tcctgtactt
    18721 tttttctcaa gatcgaagcg cccatcgcac ttgatcccat tcctgcatca caagcaaaaa
    18781 tgattttttg tacgccagag aatgtagctc cttgaggaat accttgggca cctttggaag
    18841 cttgcttagc tgcctttgtc tcttgaacag atttttcgaa gtcttcattt gtatctttcg
    18901 catccgcttt caaaattact gcagaaatac caaacgaaac agctgcggca acaatcacgc
    18961 ccaaaataac aggaaggtaa gaattgaacg gtgtcatcgc tagaatcgca atgatcgacc
    19021 ctggagaagc tggcgctctt agtccagcat ccatcaattg gaaaatgaaa cttcctgaca
    19081 ttcctccgcc tataactgat aagaataata acggtttcat cataacgtaa ggaaaataga
    19141 tttcatggat cccgcctaaa aagtgaatga tcatggctcc tggtgctgaa gattttgctg
    19201 ctcctttgcc aaacaaagta aaagcaagta atacgccaag tcctggacca ggatttgctt
    19261 caagcaagaa tagaatagat tttccagcct ctgctacttg ttcagttccc aatggtgtca
    19321 aaatcccatg attgattgca ttattcaaaa ataagatttt tgctggttca ataaaaatac
    19381 ttgtcaatgg aatcaaatgg gcagataaaa tgatctctac accttttgcc attgcttgag
    19441 tcaatgcatc gacaactgga ccaatcgcag aaaaacctaa taacgctaat ccaaaaccga
    19501 taagacctgc tgaaaaatta ttgactagca tttcaaaacc ggatttgatc cgtttttgga
    19561 atagctgatc aaatttcttg atggcatatc caccaagagg tcccatgatc atcgcgccta
    19621 gcatcattgg aatatctgtt ccgacaatca cacccatcgt tgcaattgcc ccaacaactg
    19681 atccgcggtc accagcaatt acttttccgc cagtataacc gatcaataaa ggaatcagat
    19741 aagttagcat tgggcttacc atggtagcga aagcctcatt tggtaaataa ccatctggga
    19801 taaatagagc cgttaataca ccccaggcaa tcagtgctcc aatatttggc atgaccatgc
    19861 ttgataaagt actgcctaat ttttgtaccc tcgcttttag cgaaatgttg tttttgggaa
    19921 tcgtccgttc cattttgtct ctccctctct ccttttgata ctctaattgt atccgcttac
    19981 taaaaagaga aatagtcgta ctttggcacg aaactttgtg ccaaaggttt gagtatgtca
    20041 gcaaaataaa aagatttcgg tatcagaaaa cttcaatttt aaataaaact gttattttaa
    20101 tttttttata atatgcaaac aataactttt gttagcattt aatttagatg ttttccattc
    20161 tattatgtta ttaaatttta tattggaggc ctatctatga cattactgat caaaaacatg
    20221 aacatgaaac gtggtaaaaa acgtgtgatt cgacaattga atcttttagt agaatcagga
    20281 gaagtagctg ctttgattgc cccgaacgga tttggcaaaa caactttgct gaatggcatt
    20341 gcccaactgc tgccaacaac ttatgatcaa ttgtcccttc acaacatctc tcctactgct
    20401 tatacagatt atcgccggtc tttctttttt ctagaatcaa cgaatcagtt atccgactat
    20461 ctatcggctg ttgattattt gactttgatc aaatactatt ggcagagcaa agtagaatgc
    20521 caaactgttt tccaacgatt gaagatgact gagtacctta ctttgccgat tggaaaaatg
    20581 tctctaggaa tgaaacaaca tgtcttgctt gctgcttact tgatcagtga ttcatctgtc
    20641 atgttattcg atgaaccgtt gaacggatta gacccaggaa gtattgatat cttgaatagc
    20701 ctcatcagac aatggcagaa agaggggaag acgattcttc tttcttccca cgctatcgcg
    20761 aatatccaat cgatttgttc taaagcatgt tttctaaaaa atggagaaat cgtcacagaa
    20821 tcctcaaata tggaagagat tttagctatt tatcgtaaat tatatgaggt gtaactatgt
    20881 ctttacttac atttgaacgc aatctcttac taaaaaacaa agtaaacttt ttatttcctc
    20941 tattgttggt cgtgctcttt gcattcccac ttttttttga tcataaactt gcttatacag
    21001 aattcgatga actgaatcat aactatgaag agatgcaacg tttgattgaa acgttgaaag
    21061 aagatgagaa tgagaaagaa tttgtggaaa gtttagagaa aagcaacaaa ctgatcgaag
    21121 caatcttaca tgcaaaaaac acagggaatg tccagcaaat ggtcgaagca acttatcatt
    21181 atgaaaaaga tatactggat cgactgattt ctggtcaaag acaaggaatt ccaatcattg
    21241 aacaacaaaa acgtgtcgag ttgctccgtt atatgaaaga acatcagatc caaagatatt
    21301 ctatttttga cttgccagcg catctctctt tagcaaatta ctacgaaaat attttttcag
    21361 gaatgatttc tagttttttg attttatgta taaccgcctt gtttctttct tccatcatct
    21421 cttatgaaaa aagaaagcag gtaatctctc tagtcaatct attactggat agtatggtaa
    21481 agaaacatag catccgtttt accatttatt atggtgcagc tatgctaagt ctcgtgatgc
    21541 catttttgat cgtctctata ctagtaataa tcaaaaatgg tttgggagac ttccgttatc
    21601 ctgtaggaac gatcattggt caggagatac gaatcctgcc gatgtatgag tatcttttcc
    21661 agagctttct gttcctgttg ttgtgggtgc tctttttaag tacaatcagt tttcttctat
    21721 ctgctttatt tgaacattct ctagtcaatc ttttaggtac acttctttgt cttttcttag
    21781 cagagtatcg cttgttttct tcgattggat ggatagaatc aatcagtcat tacttgccaa
    21841 cttcttatgt tgattttcaa aatgtgatca taggaggaga tattttctcg ccactagctt
    21901 cagaacaggt tactttcatg aatggtatct tgacacttgg tatatggagt atcgttttat
    21961 tattcatagg tatgggcact atctatataa aaaagagcta ttaaaacaca aaaataatag
    22021 gggcggtgac aaaaaccttg tcaccgtccc ctatttcaaa taaacggttt tctagaataa
    22081 atagttttca aaagcttgca tccgcagtaa taagaacctc ccaacaaaaa tagctcctct
    22141 tatttttatc ttggttgaag ttattacttg atgcaaggca gcttttttta ctgtgaacat
    22201 tggttatata tgtccatgtt caaaaatcat ttggacttct tcaatggttt ccgcatcact
    22261 gagcgccatt acttggtcga tgtctgaaca gaaaaaggct aaatcttgga gcaccgctaa
    22321 ctgatgttct ttttttaaag ccactacaaa taagagtgtc gcgatttttg gttcctcttt
    22381 tgttccaaag tccacaccat caggcacttg gactaaatag attccttctt ctactacttc
    22441 ttcatctttg tcagtcccat gtggcaaggc tacgaaattc ccgatataga cactcgcttt
    22501 ttcttgtctt tcgatcattg attggatata tgccggtttg attccccctt gttgggtcat
    22561 ccactggccg atctcggtca acgcttcttt ccaattttga taggattgat cgagcaatat
    22621 tttttctgct ggtagctgta tcatcttttc accttcattt ctttatttct tcaatcagga
    22681 ttttcgctaa caactgataa acgatttctt tatttcctga gcggaagatc tcggtgttca
    22741 agtcattcat gatgattgct ccactgatct ttcctaaaag aatcccgtct ttttcttgga
    22801 ttggcgaagg cgccagcata actaacatac gggacaaatc gactggctgt ctatccatcc
    22861 cttcaatggt caacggatgt ttcaaattga aaatacaaaa gatcggttta gaaatcaatg
    22921 agctggatgt atggaataac cccatatttg tatgaggaat accgataggt gcttgttcat
    22981 atcgcttaag cagccggcga tgaaccacct cttcatccgt tactagtcct tcaggaaaat
    23041 aaggaaataa gctgcgaatc gtttcggaaa tcgtctcagg attatccagt tcttggataa
    23101 aaaaacgacc gagtaattga ttgatttgat ccatgaaatg cattgtttcc tcataagtat
    23161 cttctatcat ccactcgtta ttgctgttat acgtaatttt ttgccttttt ttcttctcaa
    23221 tatgcgaaaa agcttctttt agctgcttga cttcatcttc tagcagtagc ggggagacta
    23281 aaatatattt tccaccgtat cctggtaaaa tagatgtaga aataacaaca tcatattctt
    23341 cttgtaaatc gatttttttt aaatcagcaa tccgatagaa atgaatttgt tggatgaagg
    23401 gaaaatacct tttcaatcgt agttccaaca tgctagtaga agctaatcca cttggcgaaa
    23461 atcctgcaat actgatattg ctttctttcg gacttcgttc caatgaattc gcaaaatgaa
    23521 ggaccatata agcgatctct tcttcggata attttttttc ttgaaaaatc cgctctactg
    23581 ctttacggat tgcattcgca acttcctcgt attgaaccat gattttttct aaaatcggat
    23641 tggtcagtgt ctcttcttgt aaaacggctc ttgaaaacac accagataaa tgagtcaaca
    23701 gcattttgta caaagtttca tcttcataaa aatggacatt cgtctcttca cttacttctt
    23761 gaatcagttg ttttactaaa taagcaagct gtgtatcgaa cgtctcttca aagaaattct
    23821 gatcaaatga gttcgtgaag tcatttaata atcctgcaaa aaagacgatc tcatttactg
    23881 gataaagttg ttttgtctca gaagcgatcc gtgaaaacaa tcgtttagct gtctgtagta
    23941 attctttgtt tgccagacta ttatatgtct catttgtcag taaatgattt ttcgtcacac
    24001 gctccatcgt caatgtcagc atcaaaacca aatgttcaag ctttctatca cttagattgg
    24061 tctttttttg gatcaattct tccccgatta ctttgcgtgc aagagataga tgttgcggat
    24121 caacgaatcg aaaaaaataa ttcagtgatg catcgaaaga aatactatga aaaagttcat
    24181 attcattgat ttctaattct aatacattcg ctgtcagaag ccggcggtat ttttccggtc
    24241 cttcgatctc gtatccctgg ttccgaatga tttccaaagg cagttggcga atactttctt
    24301 ccaactgttt gatatctgca taaaaagtgg tattactgat gcgatatgat tccatgaaat
    24361 gagcaagact caacggctct gtcgtcagta acaactcggc taaaatcgcg tgttgtcgtt
    24421 tagcagtgga taagtcatta cttttcccat ctgtattttc ttttaattgc tctaatactt
    24481 cttctgatgc ctcaatgaca aatgagcctc tagctgcttt tttcaaagaa gcatttaccg
    24541 atttcaatga gacttcaaga ttatctaatt cgcggtaaat agtccgttta ctgaccttca
    24601 ataaattcat cattttttgg atagatacag gtgacgggct actgagaaat tctgctaata
    24661 attgtttttc tctgggcgat aaatacatct tatatttctc ccttcacgag tttttcgtat
    24721 tcattcgtag ctacccaatg ttcaactaga actagccgag ggatcttttc ttctagctgt
    24781 tcagttacag ctagttctct ttgtgcgata taaatcgttt cttcttggat tttgattttt
    24841 tctattgatt cttttgagat aacaaactgt tttttcgctt tttttgctaa ttgacttaaa
    24901 atcgtcatgc ccatagtttg tgagccgcgt ctgttgtttt tatacaaaaa tacaatatta
    24961 gcaggcgacg ttttttgttc tgtgatatct gctatctctt ttttttcttc ccgctttgga
    25021 aattggatcg cttcttttat tcgtgacagc aagatatctg tttccaaaaa gtgatccagt
    25081 acatagattt gactttgagg tactttttct cttgcaagac ccgctagttc atgttgcacg
    25141 ataaccagct gatttacttg gttattcaat tcatagatag agctgtatcc gacagaaaga
    25201 tcgatttttt ctgctgctaa tttctttttc agcagggcgg ctcccatagc acttgatccc
    25261 atacctgagt cgcaagcaaa aatgacggaa tgaatgatag atggttccaa gatttctccc
    25321 ctttctttga ccgttttgtt ctctttttct ttatttggta atttcaatag tatcgctgca
    25381 caaatgaagg tcacaaatgt actgattgct atccctatac ttaccccagc tagttgtgga
    25441 taaggcgcat tggcaaaaat cacgagtagt gacccaggcg atacaggtgc agctaatccc
    25501 gtatctaacc attgaaatat caacgtgccg cttgccccac cagccattac agctaaaaat
    25561 agatacggtc gcagcaagac aaatggaaaa tagatttcgt gaatcccgcc aatcgcatgg
    25621 atcatcaatg ctccactcgc atttactttt tcctgttttt ctgcaaaaca aaggatagaa
    25681 agtaagatcc ctaaaccagg tccaggattt gcctccatca aaaataagga agagtacccg
    25741 actgtttgtg agaattcgat tcctaaaggt gtcaatacac catgattgat tgcattattg
    25801 aaaaatagta cttttagagg ttctaagatc agactgacaa gtggtaacag attcattgtg
    25861 actagccaag acacaacgga ggcgagcaac aagtgaacac tatttaaaac tggttcaata
    25921 aaaacgattc caattataca aataacgcta ccagtcagtc ctactagtag gttgctgaca
    25981 agcatttcat atccagcctt gaatttcaca tggaatctag aaacaaattg ttcatatacg
    26041 atgccggcaa gcggcccgat aatcatcgct cctacgattt gtggagcatt ggaacgagcg
    26101 atcaacccta aagaagcaat tgcaccggcg actgcccctt tttgtccccc taccgatttc
    26161 ccgcctgaat aactgatcaa gatgggtaaa agcagctgga tcatccaatg gcatacctct
    26221 tctaatgtgg tgtgcaaatc gccagacaca ccttttgttg ctaatgacaa gattccccaa
    26281 gcgataaaca gacttagatt tggcatgatc atttcattta agtgatgtcc agcttttctc
    26341 agttgtttca tagacacgcg actgctgttg tttgctttcc atttgtattc catctttttc
    26401 accagacttc tatttattga tggacggatt ttatgtagaa aaagcagcaa acgaaaaacc
    26461 gtctgctgct ttcagctatc tgggaactat ttgcctggtt cagacgcaat gattgctagt
    26521 tcaccttgga ccgcccactc agaaacgcca tttggaataa tacagcttgt tcctttttct
    26581 aatggatatg tttttccgtc aacgacaagg tctcctgctc cttcgattac cgtcatcaat
    26641 gtataagggg cttgtttttt gaaagatgtg attcctttaa tatcccactc atatacattg
    26701 aaaaactcag tttctaaata agtgacgatc gatgaattcc cttttcggac ttctttgatt
    26761 tgtaattctg gtgttttcgc agggacagtt gttacatcga tcgattgttg gatatgcagc
    26821 tctcttgttt gtccttgcgc atcttttctg tcgtagtcat acacgcggta agtcgtgtca
    26881 gaactttgct gtgtttctaa aatcatgatt cctttaccaa tagcatggat cgttccgctt
    26941 ggtacataaa agaaatcacc ttttttaact ggtactttct tcaaaaggtc atcccagcgc
    27001 ccatctttga tcatctcagc tagttcttct cttgtctttg catgatgacc atagatgatt
    27061 tctgcaccag gttcagcatc gatgatatac cagcattcag tttttcctaa ttcaccttca
    27121 tgtttcatcc cataggcatc atctggatgt acttgtacag atagatcatc ttctgcgtct
    27181 aagatcttga tcaataatgg aaacactggt tctgaaggat ggccaaacaa ttccttgtgt
    27241 tctgaccata gttcatctaa tcttttccct ttgaattcgc cattttctac tacactgaca
    27301 ccatgaggat gcgcactgat cgcccaatct tctccgattt tatcgttagg gatatcaaaa
    27361 ccaaagactg aacgtaaacg acttcctccc caaatttttt cttgaaaaac tggttttaaa
    27421 aacataggtt cctgcataat tcaaactcct ctcatttttc atagctatta tagcattatt
    27481 tcggtaatac cgctttctct ttcaaaaagt tttcaacaat ttcatctcga catatgagat
    27541 actccgcacg atgatcgaca ggatgtacgc gatttgacaa aaatatcaac gcttcttgtg
    27601 tttttatatc aatcaagaaa aacgtacctg tataaccagt atgaaaaaga atcgcatgac
    27661 cgtcttctac atcctttttt aagtcccatc ctaaagaacg atagccattt tttttcggtg
    27721 tttgatcttg cagtaaagaa tcaatcgtct cttcttctaa aaaacgacca ttacggattt
    27781 ttcccttgtt caaatacatt tggacaaaag taatgctgtc tttcaaatca ataaagagac
    27841 ccgcgttacc tgcatgttca gccaaaactc ttgctttcgg atcatgagtt tgtccacgca
    27901 aaacctcacc cgaaggcagt tgttctgtgg gaacgatttt tttggaagga aaaggaagaa
    27961 ataagctgtt catcattcct aatggttcca atacttgttc ttgaaagatt tccactgcag
    28021 tttcttggta aatttcctct agcatcaacc caagaagaat cgtaccggca tctgtatatt
    28081 ttacttcttt tcctagttgc tcacctggtt ctagcttcaa ataagcggcg atcaattctt
    28141 cctgagatag cttatctctg tttggtatcc atgttttgat atcagaagta tgtgtcagta
    28201 aatgacgcaa agtgattgtc tgattttcaa attcaggaag atatttatga agcggttgat
    28261 cgagttctat ctttccatct tctaatagac gtaaaactaa tgaagtggtt gcaacgactt
    28321 tcgtcagtga ggctgcatca aacaacagat cttctgtcat cagctgcttt tcaggaacga
    28381 cttgggcaaa gccggctgta aatgtttcta cttgattttc ttttatgaag gcgactgcta
    28441 cacctggata aaatccttct tccatctttt tcaaaataag ctgtttcgtg tgtggataca
    28501 tctttttctc ctgcttttct atcagattct tacagtccaa gcttaaagct taatctatga
    28561 ggaatcaacc ttttttacac aaaaaaactg cgcgaaaaac cgcgcagttt cccccggtat
    28621 actattttct ctttctatct ctagaataag cgatagtatg accgttcata aaatgacgag
    28681 acatgcgaca atcgttaaga aagaaaaagt taagcgtcgc atcgaatgtc agttttattc
    28741 ttttcttttg gctcactgac gaaccaccat tcgattccta tagcatcata gattgtgagt
    28801 actgtgtgct ttttgtctaa gaagaaatca actgatagtt gctgcaagtg agcaatcagt
    28861 ccttccatgc tttcagtatc gatcatgaat ttcagaaaat caagtccaat cacttcatct
    28921 gtaggcaaat caattgcgcc tccgctagct tctgtcaaac caacatggaa atctccttca
    28981 ttcaatacat ggatgccctc ttcttcatta tgtaccttca aaccaagtac atgattaaga
    29041 aattggcgtt cttttgttaa gttagtaaca tttaaatgga ttttttcaaa ataagaacct
    29101 gttgatagtt tactgaattt ttcttcagat tttcttaata atgcttcttg atccattgtt
    29161 tctggaattt ctctttggtc gttcttattc tcttcatggt aaatttccaa ttcattctct
    29221 tctgggtcta cgagcaaaat ccccatacgt ccatgatctt ctaaagcttc tttgattgga
    29281 taattcattt tacggatacg acaaaggata tctgccattt cttctgtgct agggataatc
    29341 aatgaaaaac ggttcagctt tttgatcacc cccatgtgat cattcgctct aggactttct
    29401 tccagcagca acatttcact tccagcttct tctgttccta aaatggctaa ttcgttttct
    29461 tctcttttca aatcaaagcc aacaacgttc ttgtaaaaat caatcatttt atctcggtct
    29521 ttcacccgaa tggctacact ttttaaatag gtggcagccc ccaattgaaa atctttcata
    29581 tttcttgctc ccctctacgt caagctttat tttaacgatg gattcttctt ctttcaagca
    29641 ataactttga ttttgttaaa aaaaatgtga gaaaattcgc attttgcttg ttttttgtaa
    29701 attaatgagt aaaaactctt ataaaaagag gttgtgaaaa agctgttctg catcaagtaa
    29761 taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggttgggc ttattaccgc
    29821 agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaaaagtcgc ttagtttcga
    29881 gtagtaagaa aaattttgtg aaaatggctt tctacatttt tgcataaatt tggcttaata
    29941 ccgagaaact ggcttttgaa caccgtttac caagaggttg tgagcctgcc gtttaactcc
    30001 gagtcacaag gagcaatttc aaaaaacagc ttctcaggtt tgttgactat tgtgacttgt
    30061 gaccgaggag ttggcaggag aacaccgttt aaggaagagg ttgtaaaatc agcggtttga
    30121 cctgagtatt agagatacaa aacggaaaaa tagcttctca tattttgccg ttttttgagc
    30181 taataccgaa ggacagctga ttgaacaccg tttattcgga aaaagggact gtgacacgac
    30241 ttttgtcaca gcccctaaaa aaaataataa atttgtacta ttctatgaag cttcttcttc
    30301 agaaaactga ctgttataaa gatctgcata gaatccgtct ttttccatca atgtatcgtg
    30361 gtttcctgtt tctacgatcg aaccatgatt catcacaata atgttgtctg catcgcgtat
    30421 tgtagacaag cggtgagcaa caacgaaact tgtccggttt tccaataggc ggttcatcgc
    30481 tttttgaatc agtatttctg ttcgtgtatc tacactagat gttgcttcat caaggatcag
    30541 gacatctgga tttgctaaga atgcccgcgc aattgtgatt agctgacgct gcccttgcga
    30601 aatattgctt gcatcctcgt ttaatacagt gttatatcct tctggcaatt tacgaacaaa
    30661 atcgtctacg tgagcagctt tagcagcctc aatgatttgt tcgtctgttg catcgtcgtt
    30721 cccatatttg atattgtcat agatacttcc tgtaaacagc caagtatctt gcagtaccat
    30781 tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg gtatctacac catcgtaacg
    30841 aatactacct ccagaaacat cgtagaaccg ttcaagaagg ttgatcagtg tcgtcttccc
    30901 tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt ttgacatcca agttaaaatc
    30961 agtcattagc aagttatctg gtgtgtagcc gaattgaaca ttttcaaaac gaactttgta
    31021 tggtgtatct ttttcttctg gaaggccaga tttttcattg gacatctctt cttcatccaa
    31081 cacttcaaat acccgttcgg ctgaagcaac tgttgattgg atagtgttca tcaaacttgc
    31141 aagttgagtg attggttgag agaactgtgt tgtatattgc aagaacgctt gaacatcccc
    31201 taatgtcatg ttcccatttg caacttttac tccgcctagt acagcgacga acacatagcc
    31261 tagattttta acgaaattca tcaacggcat gatgattgcc gaaataaatt gagctttcca
    31321 accagcggca tacagattct gattttcttt ttcaaatact tcctggtctt tttcagtatg
    31381 attgaatgtt ttaacaatgg tatgaccacc atatgtttct tctacttggt tattcaataa
    31441 acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat ttcggcgcta cgatcatcac
    31501 gatgatcaag cttaaaggga tcatcaataa cgcgactaac gtcaattgcc agctgattga
    31561 aagcatcatc acgatgaccc caatcaatgt gactgcacta gttacaaatt gagtcaaatt
    31621 ttgctgcagc gtgcttgcga tgttatccat atcattgatt gctcgagaca tgatatcccc
    31681 atttgtatgt gtatcataat aggaaatcgg tacacgattc attttggctt ccaaatcatg
    31741 acgtaattca taaactgttc gttgggaaac acgtgtcata ataaattgct gtaaaaagtt
    31801 gaatacagca gaaatcaaat acatcacgat tactgtcgca atgatctgcg cgattttatc
    31861 aaagtctatc ggaaattttg ttacttgttg gccttgttgc atctgttgag cgccagccat
    31921 tacacctttg aatatttctg tggttgcttt ccccaagacc tttggtgttt ggatttggaa
    31981 gatcacggct gctattgcta gtacaaatac agcgatgatc gcaaccatac gtttagacat
    32041 atagccaaac aatcggcgta ccgttcccca gaaattttta ggcttttcac cttttacacc
    32101 gagatttctg ccaggtccac catgtcctgg accgcctgct ggtccatgag agggacgtgt
    32161 gtttgtttta ctcatgctaa gtcctcctct cttagttgtg aatgaacgat ttcttgataa
    32221 gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc caaccagttt tccttcatct
    32281 agcacaagga tcatatctgc atccattact gtacttactc gttgagcaac taaaacggtg
    32341 atactttctt tcatattcgt tttcaatgct tggcgaagat tggcatccgt tttgaaatcc
    32401 aacgctgaga aggaatcatc aaacacatag atatctgctg ttttgactaa tgcccgtgca
    32461 attgccagac gctgtctttg tccaccagag aagtttcctc ctccttgttc cacatggcta
    32521 tctaagccat cttccatctc tgaaacaaaa tctttagctt gtgcgatttc taatgctttc
    32581 caaatcattt catctgttgc atccggctta ccatactgca tattgtctcg gatcgttcca
    32641 gagaacagaa ctgctttttg aggaacaaag cccattaatt ctcgcaaatt atattgagtc
    32701 atgtcactaa tgtccgttcc atttatttgg atcgaaccag attcgatgtc atataatcga
    32761 gggatcaaat tgactaaggt cgttttacct gaaccagtcc cccctatgat tgcgacgatc
    32821 tcgcccgact tggcttggaa atcaatatct tccaatgcaa gtttttctgc tccatgatat
    32881 cgataattga catgattgaa ggccaatgtt gcgttttttc cagcaaaaga aactgtttta
    32941 ggattttcag ggtcatggat accgattttt tcatccaaca cttcattaat acggtcagct
    33001 gaagcttgtg cacgaggcac catgataaaa atagcggaaa gcatcataaa gctgatcaag
    33061 atctgcatcg cataagtcat aaatgcaatc agattaccga cttccaaagt catatctgct
    33121 atgtagtgtc caccaaacca agtgattgct acgtttgtac cgctcatgat caatgtcatc
    33181 attggcatca tcaatgcgac gatcgtatta actttgattg ccgtatttgt ataatcttta
    33241 ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat cgaatgctcg aatcactctg
    33301 acccctgtca atccttctcg aaataccagg tttaaacggt cagttttctt ttgcatactt
    33361 ttgaataatg gaacagcaaa gtacatgatt ccgccgatca aaataatcat tactgggatg
    33421 acaactaaga agatttttgt caactgatga tctttttgat aagcaagtat gctcgcgcca
    33481 atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca tttgtgtaac catttggatt
    33541 tgagtcacat cattggtcgt tcttgtgatc aaagaggccg ttccatattt atcaaacgca
    33601 tcttttgtca gactctctga ttttttatag acatccgtac gcaattgctt acccagtttc
    33661 tgtgattcac gagtagcaaa aaatgtattt gcgatagcag ctaagatact gatcaaagaa
    33721 aagccgatca tcacaaaacc tgtatgccaa atataatcga catctccttg cgctacccct
    33781 ttatcaataa tattggaggt aagtgtcggc aaatataaat ctgccagtac ttggatcacc
    33841 atgaaaactg cagctgcaat tgctgaagtc aaggagatcc gttttacgat ttttagcatc
    33901 tagttccctt ctttctattt atatactccg tttttcagcc aagaaagttt caaattgaat
    33961 tcactgattg cttgctctac tgagtaatct tcagatgttg aaaataagcg atacgaatgc
    34021 gcaattgttg taaatacagt attcatcact aattggatca gtaattccaa ttcatgatca
    34081 ttttttactt tcagcttcgt ccgatcgatc aattcaagca actcttttgc atcttgtttg
    34141 tgttctttat gtggatcgtg aggcgttttt tggcgactcg tatgaggagc gacttttctt
    34201 gaggcacggt aatccatgtt cataaacaag ttacgataaa aagaagcatt ctcgccttcc
    34261 aaaatctcat aaatcatttt agaaaaatag gattcgaatc ctttgaatag atcaccatcg
    34321 gattctttca ttagttgtaa catatcttta gagctgtcac gtctcattgt ctcaaagtaa
    34381 taaaaataaa gatcctcttt atcttcaaaa tactgataga aactgcctct agggatatcc
    34441 gctaatttga cgatattagc aatcgaagct tcttttaagg gtactctaga aaactcgatt
    34501 ctcgcagcct cgattagtct tttttgtttt tctttaggca aatggaaaaa tgtctgtgtc
    34561 ggcatatgga ttctcctttc tttcaagatg acacattgtc atatgacgct atgtcattat
    34621 aacacctcct acctgttttg caagacattt gcatagtttt tttcattatc atccgataca
    34681 ttaaaaaagt gataagagga atccttccat ttgaattaat gagagttgcc cattaaaata
    34741 gccacatact aaacaaagga tgagataaat ggcccaatca attagtgtcc aagacttcaa
    34801 taaattgcct ctcgatgctg acagcatcgt tttagataca agaaatacct tagattacac
    34861 aaaaaaccac ttaagccact cgctttctat ccccgctgct atcttgccaa gacaattaaa
    34921 aaatctagat ccaaagaaac tttattatat tctaagctat acaggcaggc gatcagaagc
    34981 attagccgat caacttactg ctaatggttt tcgtgctgtt tatatcattg gtgggatgga
    35041 agctcttcag tcgagtgttg cttgaatatt gcttgttaga ttcaaaagag gctgtgacaa
    35101 aaaatcgtgt cacagcctct tttataccct gtcttttttt agttatttat tcttcaacct
    35161 tatcaatatg acttgatagc cttttctttt tcgagtaaac tgattttgtt agtcatagtt
    35221 ataacaatca tagcaataaa aggagaaggt aagcatatgg catctgtttt agtagtcaaa
    35281 ggtcatcccc taactgcaga agaatcgcgc acagttaagg cattgacgtc ttttttaaca
    35341 agttataaag aaaatcatcc agatgatgaa gtgactgttt tggaacttta tcgcgatgat
    35401 attccggaaa tcgatgaaga attattatca ggttgggaag cattacgtgc tggtgcagaa
    35461 ttcacttcct tatcagaaag tcaacaacaa aaaattgctc gtttcaatga attgactgat
    35521 cagttccttg ctgctgataa aatcgttatc gcaaacgcct tatggaatct aaatatccca
    35581 acaaaattaa aagcttggtt cgataccgtc aatgtagctg gaaaaacgtt ccgctataca
    35641 gaaaacgggc cagaaggatt agtgacaggt aaaaaagcac tgcacatcca atctaatggc
    35701 ggtgtataca acggacaaga ctttgcctct caatatgtaa aaggtatttt aaactttgta
    35761 ggcatcgatc aagtagatca gctattcatc gaaggaatcg attatgaccc tgaccgtgca
    35821 gatgagttga tgcaaaatgc attagataaa gctgctgctt taggtaaatc attttaaaaa
    35881 ttgagataaa tgcatacaaa agagacagag gaaaaaatct tcaaagcagc atttgtgcct
    35941 tggatatttt cccatctgtc tctttttgtt attctttctg ctttttcaac ggattttctt
    36001 cataaagaga agagattgtt gtgtaccatt caaaaacatg agtaatcact actttagctg
    36061 ctgcataaac aggaatcccc aagatcacac cgatgattcc gaacaatttc ccagaagtca
    36121 gcagaactaa taaaatcgtt actggatgga ttgctaactg attccctagt actaatggag
    36181 agatcaagcg tccttctatt gtctgctcta ctgcaaaaac gatcagtact ttgatgatca
    36241 tgaatggacc tgctacaatg gctaaaaaga tagccggaat catcgctaaa aatgatccta
    36301 agtaagggat caggtttaaa aaacccgcaa taatccccaa cgtgacagca tactctaaac
    36361 caatgaccgc aaaaccaatc ataaacatga ttgccacagc aaaagccaca gttagttgtc
    36421 ctcttatata agaagaaact tgactattca tttcactcag cacattcaac gtcggttttt
    36481 gcatgcgtgt cggtaaaaat ttgacaaaat aaggtgctaa ttgtttaccg tctttcaata
    36541 gataaaataa gataaacggc atcgtcagca gagccactaa aatagtcgct accgcgccga
    36601 caaaacttcc aagactctga actgtactct tagatatatc ttggatcata tcgcctgccg
    36661 aattcatgaa cttatcacct gctgtttcca actgttcgcg aaattggttg aacagcgggt
    36721 cccgcaaaat ctcggttaat ttattatcaa tcgtatctac atattgaggg aagttactga
    36781 tgaagctcac tgtttgttcc tgaatcttag ggatgatcac tacgctcccc catgcgatta
    36841 gtgcaacaac caaaacaaac agcccaatga tactgtaaag ccttggtact tttcgctttt
    36901 caaagtaatc aactaccgga ttcatcaagt aatacaagat tcctgctaaa atgataggca
    36961 gacctacgat tgctaaaaat tgccagattg gtgcaaacaa atatgaaact tttgtaaata
    37021 aaaagacgat cagcaatatt aacaatacaa caagtaaggc tgtgactact tgattattta
    37081 aaaaccagcg ccaaaaccac gataccggtc tgttcttctt attttttcct tccataattc
    37141 ctcctgttag ttataaaaaa tttggcttcc caaaccaata gcaggcagtc tatgcggatg
    37201 aagatagatt ccatttgcaa tttgtccttc ttctggacaa gcatcaaaaa tcaatgatac
    37261 gtgagctact gtctttaaat tcccattggc catcggaccg acataatcga tcgtatatac
    37321 ctgatcatca aaagcaattg ttccgcctgc tttcaaagaa aaaggttcgt cttttgatac
    37381 ttcttgaatc accgaatatt ttcgtaaggc agctgttgct gttttatcga acaaaatcag
    37441 catgggttct tccttgttta ttgcttctgt cccaatttct gtaatctttg cctgaatcat
    37501 tgcactctct cctttaattg ctttcaaaaa cagtataaca cattctcatt ctttagccgt
    37561 gtggaataat tatgtgatag aataaaaacg aaaactattt cattacaatt aggaggaatc
    37621 agctcatatg atcgaaaaga taattttagg aacctatacg cgtcgcgaaa gtaaaggaat
    37681 ctatacgatc gaccttgata cagaaaaaga agaattagcc aacctttcat tagctgttga
    37741 agaaaatagc cctacctatg tagcaaaaag taaagctggc aatctttaca cagttactag
    37801 tgtggatgga ttaggtggtg ccggtgcgta cgataaggat tttcattttt tgaatgcggt
    37861 aacagagtct ggcgctcctt tgtgttatgt agcggttgat gaagcacgtc aattactata
    37921 cggggcgaac tatcataaag gtgaagtaaa tgtctaccat atattaggag atggcgaact
    37981 gaaagcagca gattcaatat tccatcaaga agcgacaggt cctcataaaa accaagatca
    38041 tgcgcatgtc cactatacgg atctgactcc tgaccaacga ttagtcgtat gtgatcttgg
    38101 tacagatcgt gtgtatactt atgatgtgtc cgaaaacggc aaattgaatc ttgtaactac
    38161 cttcactgca gagccaggaa caggccctcg ccatcttgtc tttcatccaa atggacaatt
    38221 cgcttattta tttggtgaat tagacagcac agtccgcgtg ctttcttata ataaagaaac
    38281 aggtgctttt gaagaattac agaaaatatc gaccttgcca gaagaattcg acggtgaaaa
    38341 tggaggggca gctatccgca tttcaaatga tggcaagttc ctttatgctt ctaaccgagg
    38401 acataactcg attgctgtat tcgcagtttt tgaagatggt tcccacattc aaaacaagca
    38461 aatcatctca acagaagggg attttccccg cgatttcgca ctagatccaa gcaataattt
    38521 tgtcgtgtgt gccaatcaga acaccgataa tctgacatta tatcgccgtc atcctgaaac
    38581 aggattactg gaattactga aaaaagatgt ctatgtacca gaatgtgtct gtgtttattt
    38641 tgatcgttag taagaaaagg agaatgaaat gaatcagcaa gacgtattag acagactacg
    38701 ggaagaacta aatattcctt tctttgatgg gaaaatcgaa gataaagagt atagcgaaga
    38761 ggaatatcag aaactcaaag ccgatttaca gaactacttt gaacaatatg tacagaatgt
    38821 agaaaactaa tcagctttcc atactatctg aaaagaacat caggacaaga cttttccgtc
    38881 tatcctgatg ttcttttcta caaataaaaa accgccgaga tggttagact cgacggaaaa
    38941 ggagtattta ctcgattttg agtaagtaca aggttgttgt tggttatagg tatgtcatta
    39001 tcataaagga ttttgatcac tctatcataa aggtaatatt acatttatat tagataactc
    39061 tagtttttat tgttatcaaa gatttctttt attttttcaa caacaaatta gtcatttttt
    39121 ggatggcagc tgtcaattgg tctgtcgctt ggtcttgttt tgcttcagat gtattgaata
    39181 gatcttcatc tacccctgat tcaagatcga tactttggat catttcttca cagcctgcta
    39241 catatgaaag ataagctttt tcaaaatttt tatgaatgcc cattgctttt gctggtggac
    39301 gcaaagtact gactttcata agcattgccc gatatttttt tgttccttct tcaaataaca
    39361 ggatgatctc ttcaagagtc gatttgtcca gttcacttgt ttttccttca tcaattgcgt
    39421 ttctgatcac ttcatatttc ggatgcattc cttctccgat tgcttctgtt tccttgacga
    39481 tttgatcgat ggactgcaca taatagccaa tattcggtaa catgttcgtt ttcctacttt
    39541 ctccattgtt gttattattt attctacttg tttttatcct caaatgacaa gtacttgaaa
    39601 aaatttaatg gtttctatac agctcgccat tcttattcgt gctcaaagaa accatctgct
    39661 aaactatatt gtttccctgc gacaatgtca tattggatca attgatattc gtcgtaaatc
    39721 tcttgttgcg agcgattgaa ttgcgcttct ttataccact gtccgttttg atagtacagt
    39781 tctcgttcaa acgccggaag ttcctgttct aatgataaaa gcagctgata aaagccattt
    39841 gttgtctgat tcgtctgttc cattagattc gctgcaaaat aaaacggact ggtgattgca
    39901 tcgtgagtcg tttgcttttc taattctcct ttactgtcga acataagaaa ttgtgtttca
    39961 tgcaatgttt gtttgtcatt ttttgcttgg atcgtatctg aatagatccc tggaagatga
    40021 tccccccaaa aaacaactaa agtccttcta gaaagattct tcaattcttc cgtgaactgc
    40081 ttcagtgccg tgctgctata ggaaatatct tgcagataat tctctaagtc aagtgtccca
    40141 cttccatctg aagtttttgc aggatagtct agattatcat actttccatc gtagggcata
    40201 tgtgtctgca tcgtgactaa atgaatgaac tgaggtgttt tttcttcctt gagaagtgtc
    40261 attacttctt tgtaagctga ctcatcagaa atatatggat tattctctat cgtatccgta
    40321 taggtcatcg tacgctcgct gatgaactgg tcaaatccta atgtctgata aacatcctct
    40381 cttttataca tcgaagtatt gtatggatgg atcgctgtcg tctggtatga ttgtgcgttt
    40441 aacgctgaaa cgatagaagg aagctgatcc atttttggta caagcatcgt gtaaggggtg
    40501 gtgagttgcg cattaaataa cgccatagaa aaaccagtca atgcttcaaa ttcgatattt
    40561 gctgttccac cgccatagtt ttgagaaagc atattgccac tatatgtttg atcagctact
    40621 tcataataat ctgccaatgg ctcaccagat acttctacgc cgtttaatcg actaggatct
    40681 gagaaacttt cactcatcac gaacacgatg ttcggcgact cttcctctac agttttattt
    40741 ttttcatcgg ctagcttctg gtatttttca gtgatttctt tgatcttttc tttcgaatat
    40801 ccttcaggtt catccattgg ttctaccttc agattgtaca aaaacccacc gataaaacca
    40861 gtgttatagt agttcatttt ctggctgtaa ggtatccata gtgccgtttt attatatgct
    40921 tttcgcaaca aattatcggg attgttgaaa ttactgatat accctagcag tccaattgcc
    40981 acaagcagtg tcgttaatcg gatcaattgg aatcgccttc cttttttcag gcttcgaaac
    41041 atgtaccagc aaaacaatcc tatcacggtt cctgcggcag ctaagatcac tgtaaacagc
    41101 atagtgccgg tcatatcttt cagcaatccg atttcagtga tcatcttcaa atcgtctgga
    41161 taaatcggtt cttgccgata actcattttc atataatttg caaaacctaa tacaccgatg
    41221 gctacgacat acgtaaggga acctaaaggg acagatcctg ctagtgcaat cagaaaaata
    41281 agaataatca gcaagacgag acaggctaag aagaattttt ccgtatgcca agaaaaagca
    41341 aatttcaacg caaggtctac ggatagatca ttttgacacc actgcaaaaa taaattacta
    41401 aaaacaacaa gaaaaaagat actgatgatt gctgctgttc cttttaaaat ctttttctgt
    41461 ttggtcgaaa gtctgcttat atatcttttc aaatgcttgc tccttcattt ctatcaataa
    41521 aagaatgttg cttctcttat tttagttgta cgaaattcga aagtaaagaa cggaaagaag
    41581 aattaatgaa atccaaatat tctctttctt tatggccttt ctggtttctt taataagcgg
    41641 acctttcgta ctccagaaat tttttctagc tgttgtttca cttgattgag ctgttcaaaa
    41701 tcagtactgt ctatgtcaat caatatgtag acatgctcat tcttatggtt acgcgatata
    41761 tctgcgatcg ctagctcgtt ctcattcagc agagaaaata tctctgctaa tatattggtt
    41821 ttcttttgat aaaatatagt gaatcgaaac ggcgcttgga aaaacattcg tgcggatgga
    41881 taattgaccg cttctctaac cgtaccgaac aaaagataat cttttaaggc ttcaactgcc
    41941 aaacgtccgc tgtctgccaa agcttcctga gtcgatcccc ctagatgagg caacataagt
    42001 atacggtcat tttctaagaa ctcttcttct ggaaaatcag tcaagtattt agccagatgt
    42061 tgtttttcca atgcttgcat aacagcatat ttatcaacaa tttcacttct tcctacattg
    42121 ataagtacag catctttttt cattttttct atattcgcct gatcgatcaa tccttttgta
    42181 tcttctgtca acggcagcat gactacgatg taatccgagg tagaaagaac tgtttctaaa
    42241 tctgcttgcc gaacataatc caactgggca tcgcggatag aataacccaa gacatccatc
    42301 cctaagctat agcaacttag cgctactttt gtgcctatcg ctccaagacc aagaagaccg
    42361 atggttttgc cttgcaattc tcgtcccaca taagcacttc ttttgttttc tgcctgttct
    42421 aaaatattag gacctgttag tgtttgtacc atacgacttg cttcgattat cggacgggaa
    42481 gataagagca aacagcacaa aacaagctct tttactgcat tagcatttac acctggtgta
    42541 ttcatgacga tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca
    42601 ccagctcttg aaattgctag caaatctggt gtaagccatt catctttcac tttcgtacta
    42661 cgaataacta ttccttcggc tggcaaatca tctattattt tgaaatattc cccttgtaac
    42721 ttttctatct cttgttctgg aatagcatca atcaaatgaa cgtgatacat aaaaccccct
    42781 ccttactaac aagtatctcg aaatttctgc aattagacaa ctgtttacca tttgacttcc
    42841 tagaagcaaa aagagcagtt agagcagagt aggctgagtg ataaaaataa ggctatgaca
    42901 agttactgcc ataagcctta ttttcgagag caaaatattg agggtgagat atcttgataa
    42961 atggtgttcc gttagctgac attgataata agactaaatt tcaaaaaagt tgaaaagcaa
    43021 atttcgtcaa tctcttctta tttcttaaaa ctaagtggct ttttcacaac ctctttttat
    43081 ctaataacca ctgtttccaa ctcaactaat tttgcccacg tttcaatttg atctgttgtt
    43141 agatttagcg agacaacggt atgatgaccg ccacctgcct gaatccatga atggacacct
    43201 tcatggaaat tcggtttagt tttccataac acacgtgcaa caggtaaatt aggtgcttca
    43261 gtcgttggtt caaaggcttc tacttcattg atcagcagtt tataatgcgt accgaaatct
    43321 gccatcgata cgactacccc ttctcctgct ttcccatcaa atacaagacg cgccggatct
    43381 tctctatctc ccattgataa aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc
    43441 ggatctactt ccatcatatg ggattccaag attgcttctt ggccactagc tagctcgtaa
    43501 gtataatctt ccatgaatcc tgtatctttc cctcgagcca tgattttcag caaccgatca
    43561 atcgctgcag ttttccagtc gccttcccca gcaaaaccgt atccttctgc catcaaacgt
    43621 tgtacagcta gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa
    43681 gcgttatagc ctccttcttc taagaaacgg cgaataccga tttcctgctg agcttgtact
    43741 tttacatgtt cctcccatgt cttttcttca taatcaccat agtcgaaggt atacaattcc
    43801 ttgtactctt caaataattg atcgacttct tcgtctgata cgcgatcgat tacttgtaca
    43861 agatctccga taccaaaata atcaacagtc caaccaaatt gaatctgtgc ttccactttg
    43921 tctccttctg tgacacccac gtttctcatg ttgtcaccaa aacgagccac ttttattcca
    43981 aaactttcat tataggctac tgcaacatcc atccaatcag caatctcttt ttggatctca
    44041 ggtcgtttcc agtagccaac gacgatttta ttttgtttat ttaatcgagc attgatgaac
    44101 ccatattcgc ggtcaccatg agcggattga tttaaattca tgaaatccat atcgatcgtt
    44161 ttccaaggaa tgctttcgtt atattgcgta gctaaatgaa gcaatggttt ttgtagaagc
    44221 tttgtcccgc ggatccacat ttttgctggt gagaaagtat gcatccaagt gatcacacct
    44281 gctacttctt ctcgataatt gacctctttc atgatttttg tgatcgtatc agcagtaact
    44341 gctaattctt gaaggcggat tggataaggc aattggccat tttcattcag ttcatcgacc
    44401 atttcttgtg catgtttctt tacttctcgc agtgcttctt ccccatataa atgctgtgat
    44461 cctacaacaa accaaaattc tttttctcca atattcaaca taattagcca ctccttttat
    44521 ttgttttgtc cgtaatacgc attttttccg tgttttcgta aataatgttt atctaaaatc
    44581 cgttgaggca gctcttctga aaatgaattg agctgtctgg taaataaatt catcttgcat
    44641 acttcatcta gtacgactgc gttcattacc gcactttgcg catctttccc ccaagtaaat
    44701 ggaccgtgtc cgtgtaaaag tacccctgga attgctaagg gatcaatttt gcgttctttg
    44761 aatgtttcta tgataacttt tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt
    44821 gtcaaaaatc gagcacaagg taccgatcca taaaaagtat ctgcatgcgt cgtccccatc
    44881 gcaggaacat ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg
    44941 attcctccga tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt atccgaagag
    45001 ggattgagtt taccttcaat gatatgattg tccaagtcca caacgaccat gtcgtctgct
    45061 gtcatctctt cataactgac tccgctaggt ttgataacga aatatcgtgt ttcaggatca
    45121 aaagcgctca catttcccca tgtatatttg acgagtccgt gtttaggcaa gtcaagattt
    45181 gcttggtata cttcttcttt cagttgttcc agcattttct atcactcctt tttcttgcaa
    45241 ttgatgaatc gctgtttttt caatgtcgat cccctcacgg taacgtctca taaatatctg
    45301 atagcctgct atttcttctg ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac
    45361 gtcttctgcc aagaagtttt ctaaagactt cccatgtcga tccatcatat aggctgctaa
    45421 taacgcgatt ccccacgctc caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc
    45481 catagcagat gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc
    45541 cactaaacga tcaatttgga cattctcaga tttcaatata tccattccga ttctcattgc
    45601 accaaaagca ctggctaaat ggatccgcat gaaattcgct aaatcaaaac ggctacctgg
    45661 tgtacgtaca aataatggcc ttccttcatt tactcctgta atgttttcac cggaaaaata
    45721 accataagaa agtaatcccc cgcaatccgt atcgccttct agcgctttgt tgaataatac
    45781 cgtgaataat ttatctctgc ttatctccat acccaaagaa ttggtaaatt cttcaaaaat
    45841 actgacccaa gcattgatat ccgaagagca gttattcgta tgaaccatgc ctactagttc
    45901 tcctgaaggt gtagtgacca tgtcgatttc cggatggaca ttttctagtt ctttttctaa
    45961 tacgatcata gcgaaggcgg aagtaccagc cgaaatgttc cctgtccctt tggctacact
    46021 gttcgtagcg accataccgg ttcctgcatc cccttctggc ggacaaaccg ggattcctgg
    46081 tctcaaattt cccgtagggt cgatcagata agcccctgat tcgcttaact tgccagcttc
    46141 ttctcctgct actagaactt taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt
    46201 ttcgattaac tgaccaaaaa tatttttcat ttcttgatgg taggttttcg ttgaaggatc
    46261 gataggaaac atgcctgatg catctcctac gcctagtact ttctcaccag taagctgcca
    46321 atggacataa cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt cttctttatt
    46381 caaaatagat tgatacaaat gagcgatact ccatctctgc ggaatattgt attgaaattt
    46441 ttccgttaat attcgttctg cttcttcagt gattgtattt cgccaagtac gaaatggtac
    46501 tagtaactgt tccttgctat caaatgctag ataaccgtgc atcattgcac tgaacccaat
    46561 cgacccgatt gtcgtaagat tctcgccata gatttcgaat acttctgctg ccatttttcg
    46621 gtaactgacg cgcagtcctt tccaaatatc ttctaatgaa taggtccaga taccattttc
    46681 tagtttgttt tcccactcat aactaccagc ggcaatagga gtaaaagatg aatcaatcaa
    46741 tacagctttg atcctagttg atccaaactc aatccctaaa gatgtacgac catctatgac
    46801 atctaacata ctttgcgcat ttacttcctt cataatgatt taactccctc cttctcgtca
    46861 aacctgatca aaccgtcttt ttagtagcgc ttacaatgat tattgtatac ttttgtacgt
    46921 acatgtcaag cagttattta caagaataca ataaaaaaca cttgttttgt acgtacataa
    46981 gttataaaat gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat tctagaagga
    47041 gagaatactt atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc
    47101 atttggcggc attctatttg gatatgatat tggtgttatg accggcgcct tacctttttt
    47161 acagcatgat tggggtttgg caggtaaagc ttctttgata ggatggatca cgtcttcggt
    47221 catgttaggt gcgatccttg gcggctcttt atctggtctt ctttctgata agcttggacg
    47281 acgaaaaatg attcttcttt ctgcacttat ttttatggct ggttctgtcc tttctgcatc
    47341 tgcccctcac aatggttctt attttctgat tgcagcaagg attcttctag gtttagctgt
    47401 tggcgctgca tctgccctag ttcctgctta tatgtctgaa atggctcctg ctagactaag
    47461 gggaagattg tcagggatca atcaagtgat gattgcttct ggcatgctcc tttcctatgt
    47521 tgcagattat ttgctaaaag ggttgcctga aacgatggct tggcgcgtga tgttagggct
    47581 cgctgctgtc cccgcgctga ttttattttt cggtgtttta gctctaccag agtcaccgcg
    47641 ttttcttatg caatctggcc gcttggaaga ggcaaaaaga gtattgaact atattcggac
    47701 accaaaagaa gctgaacagg aattcgaaca aatccagctg aatgtcaaac aagaaaagac
    47761 aaccggaact tcttggcata ctttgttttt agaaaaatat cgttccttag tcattgccgg
    47821 tattggtgta gcagtattcc agcaatttca aggagctaat gctatttttt actatattcc
    47881 gctgatcgta gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt ggccgatcat
    47941 ccaaggaatc attttagttg ctggttcttt gctctttttg gttattgcag acaagttcaa
    48001 tcggcgaact ttattgaaaa taggcggtag cgtgatggga ctttctttca tcctaccagc
    48061 tgttttagga actgtactgg atgctcatac aaattcgttg ttgattctct tgtttttatg
    48121 tatttatgtc gcattttatt cttgcacatg ggctccactg acatgggtaa tcgtaggcga
    48181 aatattccca ctagctgtcc gcgggcgagc atctggccta gcctcttctt ttaattggat
    48241 tggttctttc ctagttggct tgctttttcc tgtgatgact gcttctatgt ctcaagaaat
    48301 cgttttcggc atttttggga tcatctgctt tttaggggtt ttatttattc aagagatcgt
    48361 accagaaact cgtgggaaat ctttagagga aatcgaacaa agtgcatcta aaaaaactta
    48421 tcctaaacgt atttctatcc atcattctta aagaggttgt gacagaagtg gtcagcttca
    48481 agaaataaga aggaaccgct tagtttccag tcacaaggaa caatttcaaa aaacagcttc
    48541 tcatgtttga tgaatattgt gacttgtgac cagttggtag gagaacaccg tttagtggag
    48601 aggtggtgaa agaaacgttc agccttgagc gatgacaaaa gctctcacaa acattgcgac
    48661 gagtaaccgt aggagcaata agaggcggta aaaacccgtc tcttattttt cgttttcttt
    48721 cactgtttcc cgttcaacta gtacaggttc atagagaata tttggctgtg cctgtccttt
    48781 ttcaatcgtt gtgacgatcc accgtgcggc atcttgtccc aacttttctt ttggatgcgt
    48841 caatgttgtc aattttttat tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat
    48901 cgataggtca tctggaatat gatagccatt ttctgtcaac ttatctagca ttaaactagc
    48961 tatttgatcg ttatagcaaa tgatgccatc taaagcttgt tcttctttca accgttgtac
    49021 tgttttttca gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa
    49081 aaaacgcaca ttatactgtt cacacgcgct gataaaacct ttcatcctgt attttccttg
    49141 aagatcgtcg atttttgtga tgaacaacag acgctgatgt ccttttttta tcaaatattc
    49201 tgctgccata aaaccgactt tcacatcatc taaacaaata taagaggcat ttaactcttc
    49261 ataagcagca ttgatcatca ccatcggaat gccttgttct ctcaaagcaa cataaagtgc
    49321 taagtttggg ttatactgat tacttttcgt aggctcaacg atcaaaccat ctaccccata
    49381 agcaatcatt ttcttcaaac actctttctc caattcgtga tcgttattcg tactggctaa
    49441 aagcaatgaa tacccagatt ctcgtaattc attttcgatt cctcggataa tcgacggaaa
    49501 aatataatca gagagatatg tggtgatgac accgatcgtt tttgttctgt tcaatggtgt
    49561 tttttctttc ttatagctgt catctacata tgtgcccgac cctttttctt tacgcagata
    49621 gccctcattt accaacacag cgattgcttg ccgaatcgta tgtctgctga cgtcatatat
    49681 ttcttgcagc ttgaattctg agggaatggt tgtatttact tcgtattcct cacgaaggat
    49741 cttttgtttt atctcttctg caatatcttg atattttgcc atttgccttg ccccttttta
    49801 tttgtacgta catatatcat aacgagtctg caaggtttat tcaagcaaaa atcaaggttt
    49861 cttgtgctca taaggcatac ggaacaggtt caccaaattc tggaaatcct tgctcattcc
    49921 acgtaaagca ttgcgcgttc gcgtgccgat taggattgtc taacggatcc cctttttggt
    49981 tcttctctgg tcgagcatga tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa
    50041 acgagttgtg accaggacca aattgcttat tttttttaga agaaacgaat actggctccg
    50101 ataatttatg ccatgattct tttaataaaa gattcttgtg ctcatcagcc cacagcaggc
    50161 ccatggcata gttttcatct gttgcactac cagaatacgt aatgaatatt ttcccattgc
    50221 gagtaaggac tgctggacct tcatttactg aaaaaccccg tttttcccat tcatactcag
    50281 gtatagaaag gaggatctgc ttccccttta acgtccaagg attatccatt tcggatagat
    50341 aaagattaga attcccagta atttcaggat cttgttgggc ccacacataa tatagctttc
    50401 cttgagaaga gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc
    50461 ccttttctat ccaatctgta ttcattggat ctatatgctg attttcaagt acgaacatgc
    50521 ggtgatgatg agattcatca cgtatagttt tatgattgct tgcagcaaag taaatatacc
    50581 attttccatt gatataatgg acttcaggtg cccagatcaa ttcactcatc ggtccttctt
    50641 catgagcctg ccatacaatc aggcttgcag catgttctaa ttcattcagt gactttgccc
    50701 ttcttaattc aatcacttgg tagcctggaa ccgatcctgt aaaataataa taaccatctg
    50761 tatgtttata tacccaagga tctgcccgtt gccgaacgat aggattatta taaagtgtct
    50821 tcatcataac aaatttcctc acattttctt tattctaatt acattccaag agtatttttc
    50881 tatccctaca tttaattcgt catgtttttc tattgaaaaa tagtttattt caggttttat
    50941 tttttctggt tcttcacgtg tatttttgtc caacagatcg ctactggtta atgtgtagtg
    51001 ctctccttga tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt
    51061 gcgattgatc aagaaaatag tcagctgatt gccgttatct acgatcacac tgtccacata
    51121 aggaacattt tttatctttt cacaatcata aacagggcaa atgatttgtt gtttcaatac
    51181 ttgaccttta gcatatttcg ataagtgttg catcacataa taagttggat tcctccatgc
    51241 ttgtccttta gcatctgtca gtattaaagg tattgtgttt actaacaatg attgacaact
    51301 aatctttacg cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa ataataatgt
    51361 atcttccata gtaaagtaac accaatcgat gggactaccg acttcaaaat ccctatgtct
    51421 tttttccggt tgggcatgta cattccattc atcaaacgct agatacattg ttttctcact
    51481 tctcaaatat ccttttacat aatcacaagt agcaacgatt tcttcaattt gacgatcgaa
    51541 tcgataagct tttgcaaggt atgtcgctgt atcatcacgc tcatttttag gaggtttagt
    51601 caagtcttct tcatcatact tatcaatata gttatgcaaa gctaaataat ctacattctc
    51661 ataagcttct tctaatgcga tacgatccca ttcagggtac gatggtaaac gtggcgtaga
    51721 gcttccaact aagattgttt caattgaatc atccactaac ttcatgactt ttgaggcttc
    51781 atttgctaac ctaccatatt cataagcact cttagccccc acttgccatg gaccgtctag
    51841 ctcattccct aaacaccata atttaacgcc aaaaggcttt ttttgcccat tttttattct
    51901 taaatcactc cagaaggttc cttttggaaa attacaatat tctactaaat tttttgcttc
    51961 atcaattcct tttgttccta aattcaccgt gaatatcggt ttcgcttgta catgactgat
    52021 ccatttaaaa aattcgtgga gtccaaaaga atttggttct atacctcgcc atgctaaatc
    52081 aatttttttt ggtcgttgtt ctttcctacc tacagtatcc acccaatcat aaccagatgt
    52141 aaagtttcct ccaggatagc gaatgacacc taattctaat tcctttacat aagagaggac
    52201 atcttgtcta aacccgtcac catcagctgt ttcatggccc ggttcataaa tccctgtata
    52261 gacgacactt cccatatgct caacaaatga gccaaacagc cgagaactga tctctcctac
    52321 cgtaaaatcc tctctgattt ttactttcac ttcttcgtcc aacagctttc cctacctttc
    52381 atttcctata gtcaagataa tagatacaag tttccatacg ccaccagcaa agataaaaat
    52441 aaaaggaggg aaaagaaata tgagcagtac aaatcctaat aacaatacgc aacaaagaat
    52501 atttctcaaa aaatcaaaaa atactttcca tgcactttgt tcaaaaattt gtaaaaatgt
    52561 gatatctttg ttttcagaaa tcacatatat ccaggataaa agaaacatgg aataaataag
    52621 taaaactaca tatccactaa ttgaaagtac gattgtctgt tcttctaata attgattaac
    52681 aagaaaataa ataatgatcg ttaacagact aaggaacaag ttcttaccaa aatgttgctt
    52741 tattccttga aagaaaaaat aaatgatatt tcctgatccc tcacctttcc attgcgccat
    52801 tacataaaaa acagcacatg tagcaggcac gatagtaata acaggcaaac acacgattac
    52861 ccagagaata cctaataaga tgtaatcaga aaccacaatc aactgtccat aaagtttagt
    52921 ctcaactatt ttcatcttcc tcactctttc attccagaca tagctgttcc ttgaacaaaa
    52981 tatttctgtg caaaaatata aaccactagt aatggcagca ttgaaataac cgttccagcc
    53041 attacaggtc cataaaaaga aatgttctgc cctgtaaata aagctaaccc tgctggtaaa
    53101 gttctcattt ttgaactact tgtcaatatc aatggataaa gcaagtcatt ccaactattc
    53161 atcagcgtga aaatccctaa tgataaaaga gtaggtttgc tcaatggcaa catgattttc
    53221 aaaaaaatat taaactcact taatccatca atgcgtgctg cctcttccaa agaatctggt
    53281 aaagtaataa aaaaggaacg catcatgaag ataccaaatg cagttgtcat acgaggaatg
    53341 atcaaacctg cataagtatc aagcatgcca agcaaattga cttcaataaa tagaggaatc
    53401 atgaagactt ggaatgggat catcattgtc agcaaaacga aatagaacaa aactgatttt
    53461 cctttgaaac gcatacgagc aaatgcatac cccgcaagag aatcaaagaa tacagaagtg
    53521 atcactgtcc ctccagcaaa aatcactgta tttttgatgt aatctaataa agggatcgtt
    53581 gtccaaacct taatatagtt atctagggta tagctatctg caaatattct aggcggaaat
    53641 gagataatgt ctttttcgtg tttaaaagaa gaaataatca gccacaaaaa aggaaacgca
    53701 ataaccaaca atataactag catactaatc gttcgaatga tttttttacc actaactgct
    53761 tttcgactat tcacagtaac tgtaatcgag ctttctggtg ccatacaggt ttccctccta
    53821 tttcatttgt ttttctttcc gtgacatgta cacattagtg gagatagcta tagttgcaac
    53881 aatgataaac aaaaccgaag acagtgctga tgcatagccc aattcataag gtgctgtaaa
    53941 tcctcgactg taaatatatt gaacagctgt ttctgtctta aattgtgggc caccagcggt
    54001 tgcaacatat acttggtcaa acacttgcat cgaaccaatc agattcgtta aaatacaaaa
    54061 acctaaggag ggaacgatct gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc
    54121 accatccatt tgagcagatt catacaaaga gggagagatt ccctgaatag cagtcaagag
    54181 aatcgtcata gtaacaccaa aattcttcca aacagtcatg atggctaccg tcggcatagc
    54241 aagggttgga tcgcgaaaaa attgagggat cggcaagccg acttgtgaaa acaagtaagg
    54301 aatcgcccct acattcggat caagcaacat agaaaaaata atcgcaattg acgtcaatga
    54361 acaaataact ggaatgtaaa atgtcgaacg acaaaaacga ttgaatctgg tatttttaga
    54421 taatgctact gctgctaata aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa
    54481 atataaagaa tgaatcaacg aattaactgc tcgttcatca ctaaatatac gtgtgaaatt
    54541 ttcaaaactt acaaaatttc ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc
    54601 aaatgttcct aaaagcggta tgacagtaaa taataataaa actaaaaaag atggagttaa
    54661 aaacaaatac gcagcacgat caggacgatt ccaatatttg gtgaatccgt tcatatagtc
    54721 cacctcatct ttcattttga taaaatatac aatcttggtt taaacgaatc tgaataagat
    54781 atccgaacaa tcgctgtgat ttttgcaata acacctatct gcaaatacta ggtcgatcat
    54841 tcagatatct gtatcttgcc aacagtctat cctgtataaa gttctgtact atttttgcag
    54901 taattgattg atggcatcat ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa
    54961 taatttttca atcagtggat tgatgatatc gttattgatc gcagagattt gggtaaaacc
    55021 tggtaaatat ggctctgcat agtccatttg tttcgataat tcagatacaa taggatcatt
    55081 ttttacttct tcatcttttg ctactgaatc caagtaggca ggaaaaccat tttcgatact
    55141 ccatttttta ccaatttcag ttgtattcca atatttcaaa aactcataga cagcttcttt
    55201 tttattatta tctgtacttg ccggaatggc atatcctacg ccatctaaaa tagctgtttt
    55261 tttgtctgaa tcactccctt gtggtaaggt agtcacatta taatcaatct cgtttgcttt
    55321 caatccgtta tttaaccatg gcccattgat ttcaatggct agttgaccag cattcattaa
    55381 attgtccatt tcggcgccag aaatcgattc tggaccagtc ttgtctacat ggatcatttt
    55441 ttggatcgct tttaacgttt taaggttctc aggagacgca aattcagact tagtataatc
    55501 atcgttcact aatttaccgc catttgctaa catccagtta tataacggtg cagttccatc
    55561 cttattgaag acaaaacctg atacattttt actactatca gttaattttg gtgccatttc
    55621 tgtcaattct tcccaagtag taggtggttg ttcagtatct aatccagctt gtttaaacaa
    55681 atcattattc caatacatat acatgccttg tacctgcatg gggacaaaat actgtttacc
    55741 ttctacttca cctaattcaa tagccgtttc tttaaaatca gatttatcta ctccatcata
    55801 ttcccagaaa tcgtctaatg gttgtaccgc tccgttttct acatactgtg caaaatctgg
    55861 ataatttaat gcaataaagt caggtgcttt tttagaagaa atcgctgtag gaagtttttc
    55921 attcaaattg gcccaagtca tgatatccat ttcaattttg atgtttttat catttgtttt
    55981 attaaattca ttaacgattt cttgcaatat ttcaccatca gatgcagtaa acccattcca
    56041 aaaagtcaaa gtcacttctt tatcagaatc agattctgct gaacttccgc caccgcaagc
    56101 tcctaaaata cctgttgcag ctaccaacaa agagccaatt aaccattttt tacctttcat
    56161 tttttgttcc tccttcatct gataaacgac atctgaccac attccaagat agcggtttca
    56221 ataaacaatt gattttccca tcttcagctt ttacatttgt tgaatgactt ggttttactt
    56281 gttcactttt aggaagatta gttgcttttg tatcaaatcc actcatttca gaaaaatcaa
    56341 tcactccctc taatgataat tcagacaagt gaacagttag attcatttct tcttctccac
    56401 ggttaactgc aaatatgaca agttctttgt catttattac ggctatactt tctaaatacg
    56461 gcacttcttc aaaatcttcc gtcgcatagt ttgctgaacg aatttgtggg cttaacacct
    56521 ctccttgtcc atacacagca gcttgcataa atggataata gatcgtctgt ctccaagctt
    56581 ctccgtttgt ttctgtcctg attggtgcaa taacatttac caattgagca agacaagcaa
    56641 tctttactct atctgcatgt ttcaataaag tgattaatag acagcctaat agtaatgcat
    56701 cttcaaaatt atagttatct tctagtaaag gaggagcaat ctgccaaggc gctgcttgct
    56761 catcttgttc cgctgaatga taccaaatat tccattcatc aaaggacagg ttgatctgct
    56821 tcttactatg tttcttcgcc ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc
    56881 gatccatgtc taaggaacgt gcaagataat gagaaagatc atttttttgg ttgccataat
    56941 attgatgcag agacaaataa tctatttgat cgtacgcttg atcaagaact gttaactccc
    57001 aatcaccaaa tgtaggcatt tgccgattcg aacttccgca taaaaccacc tcaattgaat
    57061 catctacgag tttcatcgcc ttagccgttt cctctgctaa ccgtccatat tcttccgctg
    57121 ttttatgtcc aatctgccaa ggaccgtcca tttcattccc taaacaccat gtcttaattc
    57181 caaaagggct ctcttgacca ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa
    57241 aattacaata ttcgactaaa ttacgagctg catctactcc acgagtaccc agattgacag
    57301 ccatattgat ttcggccccc acatgctgac accagttata aaattcatga atcccaactt
    57361 cattcgtttc aattgtccgc caagctaaat ccaaccttct aggtctattt gctatgggcc
    57421 caatactgtc ttcccaacga aatcctgaca caaaatttcc accaggatat ctaatcatgg
    57481 gaatgttcaa ttcttttacc aagtgaatga cgtcttggcg aaatccactt ttattagctg
    57541 atggatgatc tggctgataa atcccttcat atactgctcg tcccatatgt tcaataaatg
    57601 aactgtacaa ccgcggatca actttgccta cacgcttgac actactaatc aacattgtgg
    57661 cttccataac tgacctcctt taatttatac atgaataata cacctcgttg aaagcgttgt
    57721 caataatata aatgttaaac ttacttattt tttatcgatt ttaattaacc aaaatattaa
    57781 taatgattaa aacaaataat ttaatataac ttttattaac aaatatgtta atatataaga
    57841 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
    57901 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
    57961 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
    58021 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
    58081 tcgagccttc gtgtcgataa aatcgatatc agttttccag aaaaaatatt caatgctttt
    58141 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
    58201 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
    58261 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
    58321 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
    58381 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
    58441 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
    58501 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
    58561 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
    58621 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
    58681 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
    58741 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
    58801 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
    58861 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
    58921 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
    58981 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
    59041 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
    59101 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
    59161 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
    59221 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
    59281 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
    59341 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
    59401 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
    59461 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
    59521 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
    59581 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
    59641 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
    59701 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
    59761 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
    59821 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
    59881 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
    59941 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
    60001 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
    60061 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
    60121 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
    60181 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
    60241 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
    60301 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
    60361 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
    60421 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
    60481 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
    60541 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
    60601 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
    60661 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
    60721 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
    60781 ccttccactt ggattaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
    60841 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
    60901 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
    60961 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
    61021 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
    61081 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
    61141 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
    61201 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
    61261 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
    61321 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
    61381 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
    61441 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
    61501 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
    61561 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
    61621 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
    61681 aaaatggcga gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag
    61741 caggttacca taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt
    61801 tctatcacta caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc
    61861 tgtttcactc ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc
    61921 gcatagtcga tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt
    61981 ttatcatatg tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa
    62041 ttaatagccg tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt
    62101 aaattactat tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg
    62161 atttagatcc aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa
    62221 ttgaaccttt aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttcg
    62281 tgagaaaaaa atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt
    62341 tgtcaggagg aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca
    62401 gtccgagaac cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt
    62461 cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt
    62521 atcgaacaat ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg
    62581 accaccagaa agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg
    62641 ataccctctt gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga
    62701 gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct
    62761 atggacaaga gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat
    62821 ggatgaatat ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat
    62881 ttgatctcac tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc
    62941 aagctcataa gaagtggttc tcttacagct ggcctgaata tcagccaaaa gcgcataaag
    63001 gactaggaat gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg
    63061 gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat
    63121 gatccaacaa aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta
    63181 gatttttatg ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt
    63241 taaatatatt catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt
    63301 aagaaacaat tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa
    63361 accaagaagc tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg
    63421 cagatagctt tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac
    63481 tccaagctct ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccca
    63541 aaaaacaagt gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt
    63601 tgagttataa cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg
    63661 ctgcttcaaa taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct
    63721 tgcctgtttt cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa
    63781 taaatcatct agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc
    63841 aaattcttca agataccttc gctctttttc tttatgatag cccgtcggta aaagtgtcac
    63901 tttgtaatca taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg
    63961 atgattggta taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct
    64021 ggcatttgca ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt
    64081 ttcctcatct acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc
    64141 tgtgagcttt gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga
    64201 ttttcttgta aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct
    64261 tcatgcgaaa aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt
    64321 tcattcattg gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt
    64381 aatacggatc ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt
    64441 atctctacat aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa
    64501 acgcgtctca tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat
    64561 taccaaagtc tcttatttta aattgaatac attataatga tactgtaaat atatctttat
    64621 tattagtgaa tggagtcaag tccacaatct tgtgtaaaag ttcaacctca tcaaaagcac
    64681 aatgttttgc gttgtgaacc taactgccat gcctttcttt ctgaggagag aggtcaggag
    64741 cagtagcgca gcgactcttg atgtctctcc tcagaaagaa gagactcgca caggttcatg
    64801 gtgctaaatg ttattcgaat taaattctaa atattttcgt ggagaagcca gccactcagt
    64861 gtctttttcc atcaaaagtg ttccaattat ccgagtgatg gaattgatat ttggaaaaat
    64921 ccgaatcact cgttctctcc ggcgaatctc ttcgttcaac cgttccaaca cattggttgt
    64981 acgaatcctt cggcggatat tttcaggaaa agacaaaatt tgaatagcat cttcaaagcc
    65041 gttttctaaa atgtcgcaag cagcacttaa ttttgaatca caaccatatt tttccaagaa
    65101 gtgctctttt ctttcacgtg tcaattcaag ttcagaggct ttaaagatac tcttcaactc
    65161 gtcttttaca tcagaagaaa ctttctttgg cagcttatca aaaatatttc tcaaaaagtg
    65221 ggcttggcat ctttgccagc tcgcacctaa aaagttcttg cgaacagcct taactaaccc
    65281 tttgtgtgca tcagaaatga ccagttttac accgaacaaa cctcgttgaa cgaacgattg
    65341 aaaaacggta tcccaattat cttccgattc gccgtcttga acatcaaaac caagaatcgt
    65401 acgtcgtcca tcagaatcaa tgccgatacc aatatataca ccttttgaga caatacgatg
    65461 attttccctt actttcatat acagggcatc acacatgaaa aatggatatt ccgagccttc
    65521 taggctccgg tttctccact caaaaacaaa tggatccaac tgtttcatca gatttgaaac
    65581 gaatgattta gaaacagtcg caccatccgt tagcagttca actgttttag ttactttgcg
    65641 agtggagaca ccagaaatga ccatttcaat catggcagcg atcaaagctt tttcgcttcg
    65701 ctgatatctt tcaaagatag taggactaaa cttaccgtct ctggttcgtg gaacatgaag
    65761 ttctaacgta ccaattcgag ttgtaaacga acgatcgtag taaccatttc tttgtgattg
    65821 ccgttcttct gaacgttcat aggcatttgc ctgaatatat tgatttcgtt gttcttccat
    65881 cagctgatta aacatgatag tcataagtgt ttttgcggta tcattcgcac cgctttcgtt
    65941 tataattgct tgaatttctt cgctttcaaa agtaaaatgt acttgagtca ttgatatcag
    66001 tcctattcga gtatgttttc tgcaaaaaac attgtactcc taactgagat caatggcttt
    66061 tttgatgctt cacttttaca caattatatg gacgtaatcg tgaatggata tttactttta
    66121 atagataaaa tattataact attaaaaata actattttca ttataattac aca
//
LOCUS       DAZAJZ010000005        65157 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000005 DAZAJZ010000000
VERSION     DAZAJZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.005"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1422)
                     /locus_tag="ACG86D_000339"
     CDS             complement(<1..1422)
                     /locus_tag="ACG86D_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFN3139519.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGN"
     gene            1674..2855
                     /locus_tag="ACG86D_000340"
     CDS             1674..2855
                     /locus_tag="ACG86D_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3139520.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2915..4543)
                     /gene="treC"
                     /locus_tag="ACG86D_000341"
     CDS             complement(2915..4543)
                     /gene="treC"
                     /locus_tag="ACG86D_000341"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN3139521.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4994..7705)
                     /locus_tag="ACG86D_000342"
     CDS             complement(4994..7705)
                     /locus_tag="ACG86D_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN3139522.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7715..8416)
                     /locus_tag="ACG86D_000343"
     CDS             complement(7715..8416)
                     /locus_tag="ACG86D_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139523.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8602..9201
                     /locus_tag="ACG86D_000344"
     CDS             8602..9201
                     /locus_tag="ACG86D_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN3139524.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9282..10295
                     /locus_tag="ACG86D_000345"
     CDS             9282..10295
                     /locus_tag="ACG86D_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN3139525.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10403..11320
                     /gene="rbsK"
                     /locus_tag="ACG86D_000346"
     CDS             10403..11320
                     /gene="rbsK"
                     /locus_tag="ACG86D_000346"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN3139526.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11348..11743
                     /gene="rbsD"
                     /locus_tag="ACG86D_000347"
     CDS             11348..11743
                     /gene="rbsD"
                     /locus_tag="ACG86D_000347"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN3139527.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11743..13116
                     /locus_tag="ACG86D_000348"
     CDS             11743..13116
                     /locus_tag="ACG86D_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3139528.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13473..15617
                     /locus_tag="ACG86D_000349"
     CDS             13473..15617
                     /locus_tag="ACG86D_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3139529.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15669..16709)
                     /locus_tag="ACG86D_000350"
     CDS             complement(15669..16709)
                     /locus_tag="ACG86D_000350"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3139530.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16986..18788)
                     /locus_tag="ACG86D_000351"
     CDS             complement(16986..18788)
                     /locus_tag="ACG86D_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139531.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18802..19554)
                     /locus_tag="ACG86D_000352"
     CDS             complement(18802..19554)
                     /locus_tag="ACG86D_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139532.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19563..19925)
                     /locus_tag="ACG86D_000353"
     CDS             complement(19563..19925)
                     /locus_tag="ACG86D_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3139533.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20101..20973)
                     /locus_tag="ACG86D_000354"
     CDS             complement(20101..20973)
                     /locus_tag="ACG86D_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN3139534.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21024..21512)
                     /locus_tag="ACG86D_000355"
     CDS             complement(21024..21512)
                     /locus_tag="ACG86D_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN3139535.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21650..22441)
                     /locus_tag="ACG86D_000356"
     CDS             complement(21650..22441)
                     /locus_tag="ACG86D_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN3139536.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22496..23104)
                     /locus_tag="ACG86D_000357"
     CDS             complement(22496..23104)
                     /locus_tag="ACG86D_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN3139537.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23097..23414)
                     /locus_tag="ACG86D_000358"
     CDS             complement(23097..23414)
                     /locus_tag="ACG86D_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3139538.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23570..24298)
                     /locus_tag="ACG86D_000359"
     CDS             complement(23570..24298)
                     /locus_tag="ACG86D_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN3139539.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24457..25338
                     /locus_tag="ACG86D_000360"
     CDS             24457..25338
                     /locus_tag="ACG86D_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3139540.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25575..26618)
                     /locus_tag="ACG86D_000361"
     CDS             complement(25575..26618)
                     /locus_tag="ACG86D_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3139541.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26621..27289)
                     /locus_tag="ACG86D_000362"
     CDS             complement(26621..27289)
                     /locus_tag="ACG86D_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3139542.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27431..29767
                     /locus_tag="ACG86D_000363"
     CDS             27431..29767
                     /locus_tag="ACG86D_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN3139543.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29882..30193)
                     /locus_tag="ACG86D_000364"
     CDS             complement(29882..30193)
                     /locus_tag="ACG86D_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139544.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30374..31849
                     /locus_tag="ACG86D_000365"
     CDS             30374..31849
                     /locus_tag="ACG86D_000365"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN3139545.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31896..32480
                     /locus_tag="ACG86D_000366"
     CDS             31896..32480
                     /locus_tag="ACG86D_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN3139546.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32482..32865
                     /locus_tag="ACG86D_000367"
     CDS             32482..32865
                     /locus_tag="ACG86D_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN3139547.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33182..33466)
                     /locus_tag="ACG86D_000368"
     CDS             complement(33182..33466)
                     /locus_tag="ACG86D_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN3139548.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33818..34528)
                     /locus_tag="ACG86D_000369"
     CDS             complement(33818..34528)
                     /locus_tag="ACG86D_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN3139549.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34525..35997)
                     /locus_tag="ACG86D_000370"
     CDS             complement(34525..35997)
                     /locus_tag="ACG86D_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN3139550.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36135..36608
                     /locus_tag="ACG86D_000371"
     CDS             36135..36608
                     /locus_tag="ACG86D_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3139551.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36761..37312)
                     /locus_tag="ACG86D_000372"
     CDS             complement(36761..37312)
                     /locus_tag="ACG86D_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3139552.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37417..37947
                     /locus_tag="ACG86D_000373"
     CDS             37417..37947
                     /locus_tag="ACG86D_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN3139553.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38430..38873)
                     /locus_tag="ACG86D_000374"
     CDS             complement(38430..38873)
                     /locus_tag="ACG86D_000374"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139554.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38889..40049)
                     /locus_tag="ACG86D_000375"
     CDS             complement(38889..40049)
                     /locus_tag="ACG86D_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3139555.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40556..42256
                     /locus_tag="ACG86D_000376"
     CDS             40556..42256
                     /locus_tag="ACG86D_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139556.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42358..43191)
                     /locus_tag="ACG86D_000377"
     CDS             complement(42358..43191)
                     /locus_tag="ACG86D_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN3139557.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43495..45660)
                     /locus_tag="ACG86D_000378"
     CDS             complement(43495..45660)
                     /locus_tag="ACG86D_000378"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN3139558.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45920..48214
                     /locus_tag="ACG86D_000379"
     CDS             45920..48214
                     /locus_tag="ACG86D_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN3139559.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48207..48872
                     /gene="pgmB"
                     /locus_tag="ACG86D_000380"
     CDS             48207..48872
                     /gene="pgmB"
                     /locus_tag="ACG86D_000380"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN3139560.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48865..49872
                     /locus_tag="ACG86D_000381"
     CDS             48865..49872
                     /locus_tag="ACG86D_000381"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3139561.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49894..50913
                     /locus_tag="ACG86D_000382"
     CDS             49894..50913
                     /locus_tag="ACG86D_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3139562.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51194..52561)
                     /gene="nhaC"
                     /locus_tag="ACG86D_000383"
     CDS             complement(51194..52561)
                     /gene="nhaC"
                     /locus_tag="ACG86D_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN3139563.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52670..54091)
                     /gene="tyrP"
                     /locus_tag="ACG86D_000384"
     CDS             complement(52670..54091)
                     /gene="tyrP"
                     /locus_tag="ACG86D_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN3139564.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54334..56211)
                     /gene="tdc"
                     /locus_tag="ACG86D_000385"
     CDS             complement(54334..56211)
                     /gene="tdc"
                     /locus_tag="ACG86D_000385"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN3139565.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56502..57758)
                     /gene="tyrS"
                     /locus_tag="ACG86D_000386"
     CDS             complement(56502..57758)
                     /gene="tyrS"
                     /locus_tag="ACG86D_000386"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN3139566.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57870..58075)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58338..59639
                     /locus_tag="ACG86D_000387"
     CDS             58338..59639
                     /locus_tag="ACG86D_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN3139567.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59708..60307)
                     /locus_tag="ACG86D_000388"
     CDS             complement(59708..60307)
                     /locus_tag="ACG86D_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3139568.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60561..61442
                     /locus_tag="ACG86D_000389"
     CDS             60561..61442
                     /locus_tag="ACG86D_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3139569.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61455..63065
                     /locus_tag="ACG86D_000390"
     CDS             61455..63065
                     /locus_tag="ACG86D_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139570.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63142..63801)
                     /locus_tag="ACG86D_000391"
     CDS             complement(63142..63801)
                     /locus_tag="ACG86D_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3139571.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64084..64749)
                     /locus_tag="ACG86D_000392"
     CDS             complement(64084..64749)
                     /locus_tag="ACG86D_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN3139572.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            65027..>65157
                     /locus_tag="ACG86D_000393"
     CDS             65027..>65157
                     /locus_tag="ACG86D_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3139573.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     "
BASE COUNT        19550 a        13264 c        11751 g        20592 t
ORIGIN      
        1 atttcctgca tataaatgaa aatgttcgga gtcagccggt gcaatcgtgt gatcactgaa
       61 ttgaatatat ctaaaagcgc cactttcctt atcttctgct tcgaatagat accttacgcc
      121 acggtttcct gcctcgtagg tcaagatttc tttccctaca taacgataat ttgaacgttt
      181 cgttgttcca ttcttaaacg taaatgccat ctctttctca ctgatcttga ttgaagcaat
      241 atctgtttga tagcccttcg tataatattc tttatattct tgtgctgtct tatcctgatt
      301 caatttttct ttatattcaa aaacaggatc aaaagtccca tcttctacat acgtatctac
      361 tgtttgccaa gttcctgccc aatctgaaag agagcgatct tgtacatcct cgtcttcaaa
      421 ataaccgttg tagacgctct tttctttttt gggggtcaaa gtatcttcta gcgggttttc
      481 tgtctctgtc gtttttttca acgcttcgag gtttgcttcc atgatagaaa gatagttttc
      541 tccattttct atttgttcat tcgtcaatcc ttccaaagga ttcaatactt ctaaagatac
      601 acctgcttcg tttgctaacg ttctggcaat actatctttg gcgttttctt caaaataaat
      661 atagttgatt ccatgttctt taacaaagtg tttcagttca gcaattctcg ccgcagacgg
      721 ttcttcacta gaagaaagac cagcaatcga tacttgattc aatccatatt ctaacgctag
      781 atagttgaat gcagcatgtt gcgtgacaaa agatctttgt tttgcttgct taaaggtttc
      841 ttgataaagc tgatggagtg cttgaagttt ctcggtatag gctgctgcat tttttgtcca
      901 tacttcctgt ttttctgggt aagcttcaat cagctgatca cgtatgtttg ctacctgctt
      961 gattgctaaa cttggagcta gccatacatg tgggtctagc tcatgtgtat ggccttcttc
     1021 cccgtgttca tggtcatgat catgtccttc ttcacttccc ggaagtaaaa gcataccttc
     1081 tgtcgctttt atcgtatgga cattgccttc ttgtaatgtt ttttggattg cggggaccca
     1141 tgtttccatg ttttcatcat tataaacaaa gatatctgta tcttggatct ttgccatgtc
     1201 tttagcagaa ggttcataat cgtgggaatc agtccctgct ggaatcaata actctacttc
     1261 tccttcatcc ccgacgattg cttttgtaaa atcatacatg ggataaaagg tcgtcatcac
     1321 gctcaatttt tctttactgt cctcggcttt cccacttgcc ccgcaagcag ttaacatacc
     1381 tgctaaaacc agcatggcca atatacctat caatcgtttc atttatgaca ctcctcttct
     1441 catcttcttt catttgaatg ttccgattcg cttttcgcaa accgtaatag ttacgtttta
     1501 gcaacaggat ttaatttatc atatggttaa aagaactgtc aagcgttttc ttattttaat
     1561 tgatgagaaa gtttatatga atgattgacg atatttttgc aatcaacctc tatcattttc
     1621 cttttatacc cgcttttgtc tataatgaag ttatagataa aggaggaaaa agagtgaaaa
     1681 aagaaacaga tacaacgaat cgtttgatcc gctttttagg aggaaaaacc tcttactatg
     1741 ttttaggaat agttatctta cttgcgctga tcatttattt tttccatcag atctcgttta
     1801 tattcaagcc tgtcggaatc attatcgcta ctatcttgcc cccattattg tttgcactga
     1861 tcttgtatta tcttttggaa ccagtcatca aacgtttgag caagcacctt tcgaggaact
     1921 tgtcagtcat tatcgtttat gtgattattt tatttttatt ggggcttgga agcatatggc
     1981 ttattccttt tcttgaagaa caggcaaaga cattgatcag tagtttgcca gatttgtttc
     2041 aagattttca agagagtctc cgacaatttt tggagaaaac accttttgcc gattcattta
     2101 atcaattttt cgcttctatt gacgacgtga cgtctgatat agctggattt attggaaatt
     2161 attgggaaag tggcgcacaa cggatcagca acattttttc aactgttaca gctattttta
     2221 ttactttatt tactggtcca attgtcgctt ttttcctttt gagagatcct caaaaatttt
     2281 atcagtcagt tttagcgatt gtcccaccag catttcgtac agattttaaa aatttgacaa
     2341 agattgctaa tcaacaattg ggttcatttc taaaggggca aatcattgct tcatttatcc
     2401 ttggagcagt ctactgggtt tgtttcttac tgatcggatt agaatttgct agtgttttag
     2461 ctatttctgc tgggttgtta tgtatcattc catatattgg gccgtttatc gccttctttc
     2521 caggtttgat cattgcattc caagactcta cttttatggc tgtcaaattt gtcattgtct
     2581 ggtttgccgt acaacttctc cacggtgact tggtgattcc tcgagtaatg ggtgatcgtt
     2641 tacagatcca tccgattacg atcttaatcg ttttacttgt catgggtgat ctaatgggaa
     2701 ttgtgggcgt tatttttggt attcctatct atacgttagt caaattactc gttactttta
     2761 ttttccgtaa attcaaacaa cgctacaata aattttatgg cgataaagga gagtatgaaa
     2821 actcttcatt ttctgaagaa gattatttta aataaaacac aaaacgtcga tcttacgaga
     2881 atagctcgta ggaacgacgt tttgtgtttt tgttttaatt tttcagcaaa gcaattgcct
     2941 ggtatggttt tagctccaga ctttgatgaa gaacagtatc ggaataatta tcgataagaa
     3001 tattggcttc tgtgaatcct tctgggatgg cgatccttgt atctttatca tagaaatttg
     3061 ttaatacaag cagctgctgt ccttcaaatt ggcgaagaaa gccataaact tccggatgat
     3121 ttgcttcata agcatgataa tctccttgtg caacgattgg atatgttttt ctcagccgaa
     3181 tcaatttctt gtaataggac aaaatagaat cttcagaaga tagttctttt tctacattaa
     3241 tctcttctag cttgcctcca acttttaacc aaggtttccc tgtagtaaat cctgcttgtt
     3301 ctgtgtctga ccattgcatg ggtgttcgag agttatcgcg agattttgct ttgatccgac
     3361 gaaacgcttc tttttctgtg aatccttgtt caaggagtaa cttataagca ttcaaacttt
     3421 ctacgtcaat atagtcggaa atcgatgaat agtctgggtc gatcatgccg atttcttctc
     3481 ccatataaat atagggcgtc ccacggttca aatggatcat ggttgctagc atctctgctc
     3541 cttgttttcg atactgatcg atagctacaa atcgattgag cgcacgtggc tgatcatgat
     3601 tattaaaaaa tagagcattc cagccgttac cctcactcat cttttctccc catgtatgaa
     3661 atagtcgctt taattctgca aaatcaaatg gaggagtaga ccatttattc ccattttcat
     3721 aatcgacttt caaatgatgg aaattaaaga ccattgataa ttcttctcta ctaggttctg
     3781 tatacaagat acaattttcg atcgtcgtag agctcatttc accgactgtg atgatagtgg
     3841 aatccgtccc aaacgttgct tggttcaatt cttttagata cgtatgggtg attggtttgt
     3901 ccgtatattc aggtttgcca tcattttctt tattattttt caattgttcg tctttaccga
     3961 taacattgat aacatcgaaa cggaatcctt ttaccccttt ttccatccaa aaacgaacaa
     4021 ctttgaatag ttcctctctt actgcttggt tccgccagtt caagtcagct tgtgtccgat
     4081 catataagtg caaataatat ttccccgtat ctccaaatgg cgaccaagca cttccaccaa
     4141 attttgatac ccaatccgtt ggctcttctc ttaaaatgaa gaagtcctga taatgctggt
     4201 cccctgctag tgcttttttg aaccattcat gttctgtcga aacgtgattc agcaccatat
     4261 cgagcatgat ttctatccgg tatttctttg cttcggctat tagctgttcg aattcctcca
     4321 tcgtaccaaa gataggatcg atggctgtgt aatcagagat atcataaccg ttgtcttttt
     4381 gagggcttgg ataaataggg tttagccaaa tcatgtcgat gcctagatca ttcagatacg
     4441 ggagtttttc tttgattcct tttaaatctc caattccatc accattactg tctttgtaag
     4501 atttgggata gatttggtaa atgactttgt ctttaaatgt catggatatt tacctccatt
     4561 caactgattt attacctacc acaacttata tatacaagtt atcatttatt attataactg
     4621 aaccgaaagc gtttgtaaaa tttatcttta tacattctaa gaggtctgtt gcccaataaa
     4681 aaacaagaga tcagacatgt atttgtctat cctcttgttt aaaatagcaa aatattcttg
     4741 tttcttctat aaatggtgtt caaaagcttt tatctacggc aatcagtctt ctcaaatgta
     4801 aaacatgagg agctaatttt atttgagtat tctttttgct cgatgcagga cggctttttt
     4861 acaacctctg attcatggtg ttcaatcagc tgtccttcgg tattagctca aaaaacggca
     4921 aaatatgaaa aactattttg ccgtttgttt ctctaatact caggtcaaac cgctgatttc
     4981 acaacctctg tttttattca ttcgatttca acgcatcaat catatcgata tgctttaatt
     5041 tgaaatgggt aacaatcatc acaataaccg taaatacgat agttaatcct gctgataaga
     5101 cataagccca ccatgtgatc actaatggaa agatgacatt ctccatagac gcttgttgca
     5161 agatgaagtc agtcaatata tatcctacac caaatcctcc gatgattcct aaaagagtaa
     5221 agatgatatt ttctcgaaca atgtacatcg tgacttcttt atcaaaaaat ccaaggactt
     5281 ttattgtaga taattcgcgt acacgctcgg aaatattgat attagttaga ttatacaaga
     5341 cgataaaagc cagcagtcct gataatacga caaagattaa gacaaccgag tctaagttcg
     5401 ccattgattc ttcttgcgtt tcgatttgtt tggacatgaa agacgtattt gtcacagcgt
     5461 ctgtggcaag cagtttctcg gaaagcattt cttcttcttt tgttgtcatc ttatctgtac
     5521 gcagcaaaaa tgcattatcc atcatcgatt gatcagtaac tgaagcataa tattcagggg
     5581 acatataaat aaagctacct agatagtttt tcacgatttc ttttacttcg actttccatt
     5641 ctttttgatc ttcatcataa agcgaaatat gatctcctgt agataagtca aatagttctg
     5701 ctactttttg cgtaatagcg attcctgttt taggcaagga gcgtgggttc tcttcaagat
     5761 cagttaaatg gataaaacga tccaaatcag ctgttttact tggtacgatc actgtcagac
     5821 tttgcgaagc atgtcctttt tttctcaatt ctacggtttg agtatgaata gctaggatct
     5881 catttacttt ttcttgcttc tgcagcatat ccgctgcttt ttgacgacta tcttccgtgg
     5941 atgaatccaa tgtaacgatt gcttgatagt caatgatcgg tccaaattgt ttggctgaaa
     6001 cagaacttag agaatctttc aatcccacgc ctgcaaccat cagcccggca catccagcaa
     6061 ttccgataat cgccataagc attcttgact tatatcggaa gagatttcgg taactgattt
     6121 tttgattaaa gctaaggcgt gaccacagcg gagtgatgta ttcaagaaaa atgcgtttcc
     6181 ctggcttcgg tgctttcggt tgaagcaatg cagcaggccg ttctcttaaa tctttgaata
     6241 aaacgatcat tgcagcgcca aatgcggcaa tgaagaatgc ggcagctgct tgtaaaatcg
     6301 gcggccagtc ataaaaaata agcgcactcc caatatcata ccgctcgtta gacagctcaa
     6361 aaatgatcct tggcaaaaga ttcgttccta atatcgttcc taaaacaata cctatccctg
     6421 aggctagact tgcatagatt gcatattttt ttgcgatttc aactttcgta taacctaacg
     6481 cctttaatgt cccgatttcc ctgcggtttt cttctaccat acgtgtcatt gtcgtaaagg
     6541 tgatcaatgc ggcaataaag aaaaagaaaa caggaaaaac attggcaatc gcagcgatac
     6601 gatcagacag atcaccaaat tcttgaaaac cgacattttc tgaacgttca ttgtataggt
     6661 atgatggttt agttaaattt tcaatttttt ctgcatttcg attcaattct tcctctgcag
     6721 aggccagttc acgttctttc tcaactaatt gcctttcttg ttcagcaagt tgagcttctt
     6781 gttgagataa tgtttgaaca gcgattgttc gttgcgcttc aggaagctgc tggatagttg
     6841 ctgactgttg cgctaactgt tttttagcat cttctaaccg tgttcttgct tgcgttgttt
     6901 gtgctttgcc atccgctatt ttttgctgtg ccggttctaa ttcttcgttg gccgattttt
     6961 tcagctcttc taagcgttct tcgggtcgat cttgtaaata cttttctgcc ttttcttgat
     7021 tttgctccat tttatctttg taagctttgc tgtacgtatt caaattcttt acgtttgtaa
     7081 aatcaagata taagacagaa taaatatctg ttttaaaaac ggattcaggt aaatagacaa
     7141 aataatcgac gctaccattt ccgacgttcg cgtatcctct ctccaaattg ttgatgaaca
     7201 atggagaacg gacaaagccc gtgatggtat actctttttc tttcaattca tctgaatcaa
     7261 tctgataaat atcaccgatt ttataccctt catcttccgc tttttcatcc aatacaagct
     7321 ggtttggttt ttccgggagt tttccatcag ttaattgtag cttgttctgc ttgttatttt
     7381 tgttataaga aaatatttga atcacttcgt ttttgccagt attggaataa tagatctgtt
     7441 ttcctgtttc tacttttgca tctgggatct tttctgcttg agcaatatct ttttgtgtca
     7501 agccaccagt agaaacgatt tgcaaatcag aaagtcctgc tgtaccaaca tagtgatcca
     7561 tcgttttttg catgacaggt ccagcagttt tgacacctac aaataaaagt gttcctagca
     7621 tgacaataag gatgattgca atgaatcgtc ctttagattg ccgaatttcg cgaaaacctg
     7681 ctttaagata tgttcttgtt ttcataaaat ccccctacca tacaatctca tcaatggatg
     7741 aaggatgatc atttaattca acagacctta ctttagcatc attaatgtga atgactctat
     7801 cagcgatggg cgctaaagca gaattatgtg taatgatcaa aacggtattg ccgtgttgtc
     7861 tgctagcgtt ttgcaataat ttcaatactt gcttgcccgt ttcaaaatcc agcgcgcctg
     7921 tcggttcatc acataaaaga agtttgggat ttttagctag tgcacgagca attgaaaccc
     7981 tctgctgttc gcctcctgat aattgggacg gaaagttatt caatcgatga gctaggccca
     8041 cttgcctcaa tacctctaca ggatcaagcg catcaggaga aacttccgtt gccaattcca
     8101 cattttcttt tgctgttaga ttaggaacga gattgtagaa ttggaaaacg aaccctacat
     8161 ctgttcgtcg gtaagcagta agctgtttat ctgaaaattg cgcaatatct gtgtcatcaa
     8221 taatgatctg accttcatca ggtgtatcca tcccacctaa aatattaaga atcgttgact
     8281 ttcctgctcc acttggacca agtataatca ctaattcgcc tttttcaatt gaaaaagaaa
     8341 tatcgtcatt tgctgtaatg gtcgtttctc ccattttgta tcgtttgtat tcatttttta
     8401 cttcgacata cttcactctg attcctccat tttcacgata tttctcgcta tatctatgca
     8461 tcgaatgcgt tttctcacac ttatttattt taccactgaa tttctctaga ctaaagaaag
     8521 gaatccttta atttagttga caaataaacg atttatgatt tacttacata aaaaacattg
     8581 ttatttgaaa gaaggaacat catgaatgat ttaaaggaaa atatgatttt tgatttatca
     8641 aaaactgctt ttgtcgcgat cgacatgcaa aaagagatcg taaataacgg tacactctct
     8701 ccacacactg cagcatctat tctgacaaac aatgatttgt tagtaagaac attgaaacgt
     8761 accgctgcct tgaaagtttt agtgaacgta gacatctcca ctttccctta tttgagtcaa
     8821 caaactgata tgggtggaat gactgcttct gtacctccag aattcactga tttacttttg
     8881 aaggattctt tgaaagatac agataatatg ttgactatca caaaatacaa tccaagtgct
     8941 ttttttggta cttctcttga tcttcaactt cgccggcgtg gaatcgaaac tatcatttta
     9001 agcggtgtag cgacaacaaa cggtgtatat gctactgcat tagatgcctt tcaacacggg
     9061 taccatatcg tgcttgctga agatgcttgc agtgatcgtg acaaagaatc ccatcaattg
     9121 ttcataaaaa agatatttcc gaaaactgca cgagtacgct caacgaaaca aataatcgaa
     9181 gctatccaac aaagcaaata agtactggca cacgtgattt tcatttactt ctttttgatt
     9241 tcatgggaaa ataaccacag gatttagata ggagtatgaa catggtgaaa aaaaagatca
     9301 caatcaaaga agtcgctaaa ctttccggcg tttcaatcac aactgtctcc ttgatattaa
     9361 atggaaatac ggagaaattc agtccgaaaa ctgttcagaa agttttagct gccaaagaag
     9421 aattaggcta tcagcctgac tattttgcta gacaaatgat taccaaggaa acgaaaacaa
     9481 ttggtgtact cgttcctgat atcacgaatc cattcttcag tacactgatg cgaggaattg
     9541 aggatatctt gtataagcag aatttcgtca cgatattatg taatgctgat tctgatcatc
     9601 aaaaagaaat cgaatatcta gccgagctca cacgtcgagg tgtggatgga ttcattatcg
     9661 cgacttctgc tgtctctaca gatgcaatca acgaaaattt gaaaaaacaa ggccggccct
     9721 tcattgtttt ggatcagaaa aaatctgaag gatttagtga tgccgtacga acagacgatt
     9781 ttcgcggtgg ttacctagcg ggcatgcact tgcttagtct aggacatcaa acgatcgccc
     9841 ttgtctatcc tgaaaatcca ccggaaaatg tccacgctcg gattgagggg tttaaatctg
     9901 ctcttgatgt ttaccaaatc cctcacgacc aactgatcct cttacctaca cagttctcaa
     9961 aacaaggtgg ctaccagatc acagcagaac ttcttgattc tgctgcaact ggtgtgtttg
    10021 ctttaaatga tgaattagct tttggattat atcgcggatt agaggaagct ggaaaaagca
    10081 tccctgaaga ctacagcatt attggctatg ataatataga tatgtgcgag tatatcaagc
    10141 cgaaactgac aacgatcgca caaccaatct tcgaattggg acaaacatct gccaagttat
    10201 tgttagatcg gatccaattc ccagaaaaag aatgggaaga aaaaagatta cctgtacgat
    10261 tagaaaaaag attttcaact gcacccttga aatagctttc aaggtgtgct atatttttat
    10321 cgaactatta aaacgtttta ctaaaacgtt ttaaaaaata attttcccat caaaataaat
    10381 gagatagttg gaggtttgta aaatgaacac ggttacagta attggaagta ttaatatgga
    10441 cactaccctt cgtttaacca acatgcccaa gccaggcgaa acgatgcatg cgcatgagat
    10501 ttttcacgct ggcggaggta aaggtgccaa ccaagcagtg gcagccaaac gttcaggcgc
    10561 acggacatct tttattggag gcgttggggc tgacagcgaa ggacaacagt tattagatct
    10621 actgacaaaa gaaaacatcg atacatctgg gatagctgaa atccaaggcg ccacaaccgg
    10681 acaagcaatg attatggtag acgcagcagg agaaaatagt atcttgatcc atgcaggggc
    10741 aaataatgct ttccatgaac aagaagtcct aaaaaacaag caactgatta caaacagtga
    10801 ctttattatt gctcagtttg aaagtagctt agatgcgacg atcctagctt tttctattgc
    10861 aaaagacgca ggaaaaacaa cgatcctgaa tcctgcacca gctcgtgaaa cgattcctac
    10921 tgaactactt gaaaagacag atatcattat accaaatgaa acagaaacag aaatcattac
    10981 aggaatcaga gtgacagatc ataacagttt ggtcgcagca gccgaaaaac tccatgaatt
    11041 aggtattgga acagtaatca ttacactagg cagtgccggc gctttctatc atacagaaaa
    11101 agaacacggt atcgtccctg cttttaaagt ggatgccgtt gacacaacag ccgcaggtga
    11161 tacatttatt ggcgcattaa gcagcacact tcagccagac cttagtaact tgaaagaagc
    11221 gatcctttat ggaaatctag cctcttctgt tgctgtgcag tcttatggtg cgcaaccttc
    11281 tatcccttac cgcgaagctt tagaacaatc agcagtctaa aattaacaat gagaggaaga
    11341 aacaaacatg aaaaaaggaa aagtaatcaa ttcagatatt tcacgagtaa tcgcacaaat
    11401 ggggcacttt gataaattaa gtatcgggga tgcaggcatg cctgtcccct ttggtactga
    11461 aaaaatagat ttggctgtct caaacggtct tccaagtttt ttatctgttt tagaaaacgt
    11521 tttagaagaa cttgaggtac aatcgattga attggcggaa gaaataaaga caatgaaccc
    11581 ggaaatattg gaaaaaatca aattattgtt accagataca ccgattagct ttatcccaca
    11641 tgaagaaatg aaaaaagagt tgaataactg tcatgcattt attcgtacag gcgaaatgac
    11701 accttacgcc aatattatat tagtgagtgg agtggtcttc taatggaaac gacaatgaat
    11761 cctgaacaga tggtactgtc cgagcagatt catatgctga ttgaacagtt gggaggaaaa
    11821 gacaatattg attctgtcct tccatgtgcg actcggcttc gtatcaaatt gaaaaaacag
    11881 ccagtagcca atgcagaaaa attaaaacag caaatccctt ccattaagta tttcatttta
    11941 ctcgatagat atgcagaaat aatcatggat cagggattgg ctcctgctta caaggaattg
    12001 acgcgtcaaa tcgatcattc agctccgaaa gaaaagctaa atccgatcga aaaactattt
    12061 ggtttgatat cggaaatctt tacgccgatc attccgctgt ttgccggttc tggtatatta
    12121 aaaggtctgg ttgttttagc gactacgatt ggctggctta gtgaaacaag cggaacttat
    12181 catattttgt ctgctgcgtc gaatgccgtc ttttattttc tgccaatctt tcttgctttt
    12241 tctgctgcca aaacgtttaa agtgaatgga tatattgcgg ctgtcattgc tgcagcactg
    12301 attgaaccga atctgacctc tttactttca gacagtggca aagcagctac tgattttctg
    12361 gggattcctg tagtactgat gcagtatacg tctactgtca tgccggcgat tttaggtatt
    12421 ttcttttatt cttttgttga aaaattcctc aaaaaatata ttcgcgaaaa tatgcaatta
    12481 gtatttgtac ctttactttc attagttatc acggttcctt tgacgttaat ggtattcggg
    12541 cctgcgggtg tttaccttgg cgaaggttta gctgccgcaa ttacttggat gatggatatt
    12601 aacggaccga tcagtggcgc aatcatcgct ggtggttgga atattttggt aatatttggt
    12661 atccaatggg ctgtaaaccc tgtcatgatc agtaatatct ctgcttatgg gttcgaccga
    12721 atcgttccgt tgacaggagc agccaatttt ggaatggctg gtgcagcttt aggtgtattt
    12781 ctgcgaagta aacgttcaaa aacccgttcg atctccggtt ctgcttttgc ttcgatttta
    12841 cttgcgggag tgaccgaacc aactgtttac ggaattgcga tcccattgaa aaaaccattt
    12901 gttgctgctt gcattggtgc agctgctggt ggagctgtaa tgggatttgc ccaagtgaaa
    12961 gcgatcgctt ttgtatttgg tagtctgacc acattgcctg cattcatttc cggtactttt
    13021 ttctggtact tagcaggact ggctgttagt ctagtcgtag caatgatcac tactctcgtt
    13081 agcgggtttg atgaagacct aatgtcttat gaatagttaa tgaaaaagac tgaagtatga
    13141 gatgtttatc acatatttca gtctttttct ttttaggatc attattttta taaatttttg
    13201 tgatactttc caaactttgg tataatttac atatgcataa ccatttttat ccccacttgt
    13261 ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg tcaaagaaaa acgaatgaat
    13321 gctcgttgct tatttacaaa ttgttttatt tagaatacac agacagattt atgaaaagaa
    13381 aaagcttttc gacacgatta tctgttccaa aaaatgtcta atgaatgctg aataagacag
    13441 aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa taaaaaaacg atacgtttgc
    13501 tagctgctac ttggctgatt gctccgtctg ttttgtcgac caataccgta tttgccgtag
    13561 ataatcaaat agctgatcaa acagtcacaa cagatacgac tacatccagc tcagctacta
    13621 gtgaaacaac ggattctttt acagaagacc caactggaac aactaactca acaacaggat
    13681 cgaccgagcc aactgaaatt gatccggctg atccatcaga aaatgaaaag ccagccgaga
    13741 catattccct tacaattgct ccttctttga ttggtaagat ccaactgact gcttcgacag
    13801 cggattcccc tgaacagcaa cctttggata ttacggaaag tggaacggta gaaggtttat
    13861 tagcggaaac gaaggtaact tacactatta cgccagctga aggtgaacaa attgcttcag
    13921 tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa gacctttaca gtaggtacga
    13981 acaatttatc tattactgca gaatttaaag ctggaccgga tacttcaaca gagaaaccaa
    14041 ctgatcctag tgaagaagtc cctagtacac cagatccgga aacgccaaac aaaccagatg
    14101 tgggtaataa caacaataat aatggttcaa ataattcaaa caataataac aataattcga
    14161 acagcgggaa ttcttcgaat caaacaaacc catctggatc gaataacaat ggaaatactc
    14221 agagagaacc aaaccatcca aacaatggtt catcgaatgg cggaacgaac aatgcttcat
    14281 ctcgtccaaa tacagcaaat caaagcggta tagagaatcc atcttctgct tcttctgatt
    14341 ttgttgtaaa atcaccaatt gatgctgtat tgcctacaaa tacaacgaat gtccaacaag
    14401 caatcgttcg agaagcattc aagcatcttg gaaaaccata tgtatgggga gcaaaaggtc
    14461 caaatacttt tgactgttct ggtttgactt attacgtata tatgaaagca accggtcatt
    14521 atatcggtgg ctggacaggc gaacagcaat atgcaggtac gcagattcca gtaagtcagg
    14581 cccaacctgg cgacctcgtc ttctggggac cttcttccgg tgttactcac catgtaggta
    14641 tctatattgg gaacggacaa tttatccatg cgcctcaacc aggagataaa gttcgagtaa
    14701 cttctatttc agatttcacg cctgattttg ctgtccgagt aaacttagca ggtttacctg
    14761 ctgcttcagg ttcgctgaca ggcggctcat ctattttaga tggattagat ggctcgttcc
    14821 atttcagtca aaaccaaaca actgatcagt tcttgaaaaa gattgcagat gatgcacagg
    14881 aaatcgggca aaaagaagga atctacgcat cagtcatgat ggctcaagct atccttgaaa
    14941 gtggatctgg aaacagctta ttatcaagcg aaccaaatca taaccttttt ggtatcaaag
    15001 gatcatataa aggaagcagc gtgaccttca atacacttga acaagatagt tccggacaaa
    15061 gctaccaaat ccgcgcacaa ttccgtaaat atccttctta caaggaaagt ttggaagact
    15121 atgccgactt gatcaaaaat ggactgactg gcaatcctga tttctacaaa ccaacttgga
    15181 agtcggaaac aaaagactac aaagaagcaa ccaaatattt ggaaggacgt tatgctacag
    15241 atcgccaata ttcacaaaaa ttgaacgcga tcatcgaagc gtacgacctg acaaaatatg
    15301 atgaaccgaa aaaagaagca gcaacaactg aagaagcaaa cgctgaaatc ctcaatatgt
    15361 ctaagcgatt cgatgtgcca atcaaatgga caacagatgg cgtgccaatc acaggattag
    15421 aattttcaac gataaaacgc gtcaacaacg cttcttccat cttccgtgtc gcttcaatct
    15481 gggatttgtg gaacaatttt acggcaagac agataccaga aacaactact cgtacagtta
    15541 aagctgcaac tgttcctgta ctaaccttgt taaggttaga caaatcattc tggcttgatc
    15601 tgcctttaaa gaaatagaat aaattaaaaa ggggatgtga cactgtcaca ctcccttttt
    15661 aatttattct atttctttat atttattttg tattccgtta caaactcgcg tacttggtta
    15721 ggttccagag tgacatctcc aaaactttca agtcgctccg ctcctggcag ttcttgagtc
    15781 tcaaatgtga taccgccatg atttactaat ttattcccgc gcatttccgg tccatcttct
    15841 ccaaaatttg ctgtaaaaat cacgattcct gaagcatctg tttttacttg gatcgatact
    15901 ttttcatctg ggcttgtcaa ttccgcagca tttagtaaac cagattgttt tttcaacaaa
    15961 aatggatgat cgatgccgcc cactatcatt gattggcgaa aatcactggc aaagacctca
    16021 cctaattttt taggacgttg gaagtcaaat gctgtgccac ttactgaaac tacttctcct
    16081 gtaggcgttg tatctttatt gagcgtagca aataatcggc tattcaacca aagtatatgt
    16141 tcgtcgattg actgtgctgg atcaccggtt aaattgaaat agacatgatt tgtaggattg
    16201 aatactgttt tgcggtcact gatcccttgg accgtcactc gccatgtatc atcgttcgtc
    16261 agcgtatacc gtacttctac atctaaagtt ccaggaaatc cgttttccat atctgggcta
    16321 gtctgataga aaatgaccga tgcttcgttt tcaccattga tgactgtata atgccactgc
    16381 ttggcttcaa agctaggtgc accaccatgt aaacaatgac cagtgtccgg gtctgttttt
    16441 acttcgaacg tttcatcatc taatttgaat gtgccattcg taatccgtcc agccatacgt
    16501 ccgattgaag caccgatata agggtctttt tctagatatt cttttgcttc atcaaaacct
    16561 aagatcaact ctcgtttttc attatcgatt attgtttgaa aatgaacaac ccgtgcccca
    16621 agatcactga cagacaaaga tgtcccattt tgattcgtga tcgtaatcaa gtgatagttt
    16681 tcgccaaaat tttgttcatt gatttccatg ttccttgcac cctcttttct ttcatttgca
    16741 ctgattccag tatacatgga aaccgttttc ttaacagctt attccgtgag taacgagaaa
    16801 aaatttacta tattctaata aaagaccgtg aaaaaaaggt tgtgacaaaa atattgtcac
    16861 aacctctttt tccgaacaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    16921 aaacgaaaaa tatgaggagc tatttttgct tggctgttct tattacttga tgcaggacag
    16981 ctttttcaca atctcttttt caatacgatt ccagtatata accatgtcgt cagtacatag
    17041 tagatgaaat acaatacgaa gaatatcgta aacggaaccc aaatatcttt gtatggattt
    17101 tgcatgaata gaccaaacat ttgtaatccg aataagacat ggactacacc taagattcct
    17161 ggtgctaaga ataacacgcc tacttctcta cggatcgatt gtttaagcaa cccgcgtcgt
    17221 gttcctatct tttcaagcat ttcataacga gcaatgtcac tcttgcttcc tgacaagatc
    17281 ttgaacatca aacagctagc aagcatggtc aagaaagcta aacctaggaa gaatcccata
    17341 aattcaaaac cagagaatag accattatac acttcataca tttcatattt ctggttgctg
    17401 tactctactt tttgcaaaga tggattgttc attttatttt gagcaacaag tgtttcgatt
    17461 ggtttcaggt ttgcttggaa gtcttttacc tgaactagct gtaaagtgga ttgtggcaag
    17521 ttcagttgat caaattctga ttgactgact accttgattt ctttttcttg ctgttccggc
    17581 aattcatagc ttcgaagttc ctcttgtaca gtcagttcat tcattttttt agcaggaact
    17641 ttttctttcg tgatcttcgg cggtgttcca tcttcatgtt ttatcatgat caatggttga
    17701 gccgcaaatt cacttgcatt atagtaaatc gttcccgcat cttctttttg agtataagaa
    17761 gcagaaagtg ttggtgaaag ttctttgatt ttttgctgat cttctgcccg aggattattc
    17821 atcactagat catatccaga attgttttct gtcaagtctg caatttcatt tttaaaacct
    17881 aatcctaccg tcaaagcgcc cagtgccaag gcaaacagca tagcgaccat tgagagcatt
    17941 tgtgtatatt cacgaatacg aaagtttaac tgtgacaatg tgaaattgtt caattttttc
    18001 aatgaaatct gttctgattt ctttagtaat gataaaaaga aaatgatcac agaatggaaa
    18061 atgaaataag ttcctaatac gatcgtaact aaggctaccc ctataccaaa gacttgatag
    18121 gtccccacgt gtttcatcat atagtaaccg cagcctagaa aagcaattcc cagtactact
    18181 tcaaggaaaa acaagaattt atgctgcttg attcttgttg gtgtctgatc cgcacgaatc
    18241 aatgctaaaa tcggtttttt gacaatcgaa aaagcattga caacagcagc taataaaaac
    18301 aaaacagcaa aaaacaaaac agtgatcaac aacccgtgaa tactaaaagg tgtaaaatgc
    18361 gaaatattga gatctaactg atccactaat aatcggtgga cgatacttgt caatcctaca
    18421 ccgacagctg aacccactaa agtagctgcg atccctacta agaatgtttc agaaaaaatc
    18481 atctgtgcga tttttctgcc ttttgctccc agcatcataa acattgcata gtctttttgc
    18541 cgcatcgtca tcaaaaagga atttgcatat aaaatataaa cgaatgtgat gatccctagt
    18601 aaaacagaac cgaatcggaa aatgataact gtagccgcaa cgatcgtatt acttttcaaa
    18661 aaagcttcgt tgcttgccat tgattcaaac atgtaaaaaa tagcagatgc cataactaat
    18721 cctgagaata acaccaagta gtctcttaaa cggctcttga tcccagtaag tgataatttc
    18781 caaagcatag tcaatctccc cctactgttc caagtttcca agaatagata gtatttctcg
    18841 atagaaggaa tcacggcttt gatctccacg tttcacttct tgatggatac cgccatcctt
    18901 gatgaataaa atacgttggc aataacttgc agaaaacgga tcatgcgtca caagaagaat
    18961 cgatacatga tcttttgtat tcaattcgtc catcgtatct aaaagatccc gggcactttt
    19021 ggaatccagc gcacctgtag gctcatcccc taatagaatc gttggttgtg tgatcaatgc
    19081 acgtgcggcg gcgacccgtt gtttttgtcc accggaaatc tcagaaggat agctttccaa
    19141 aatatgagag atggataaac gttctgcaat tttttggaca cgttgcttga tttcttttgg
    19201 tctgacgcct tgtaaagaaa gaggaacagc gatattttct tgtgctgtca agctttctaa
    19261 taaattgaaa tcttggaaaa taaagccaat ctcattcgca cgaaaatctg ctagttgatt
    19321 tccttttaat gtggtcacat ctttttgatt gattcggata tgcccatctg ttggtttatc
    19381 taatgtggat aaaatattca aaagtgtcga cttaccagaa ccagaagcgc ccatgatccc
    19441 aataaattcg cctttttcta catcgaagct gatgccgctt aatgcttgcg tctttttctc
    19501 attgtttttt ccgtatactt ttgttacatt ctgtacttcg actattttgt tcatttcttt
    19561 ccctaccctt taattagttt ttccagtgct gttttcggca cgtctttttt ttcgacttct
    19621 tcatacgaag tgacaccttt atttttgttc caggtgattt ttaaatatgc tcctttacga
    19681 agcggtcgag tcaattgtcc cttgaagtcc agttcttttt ccttaccctc tttcgtatag
    19741 ccaggcagga tatatctata gttgactgtt tcttgtccag agtctgattt tgaaactgtt
    19801 tttgtaccat ctgtcgtaat ttgtacatag tattcttctc cacccataac tacgtaatct
    19861 gcgattttca cccctgcaag taccacaacg atgaaaacaa gtagaccgat cagaagtttt
    19921 ttcacttttt ccccttctct cttactttat atactgatta tagaaaaaag cgaacaattc
    19981 tgccttcgaa tcaactatca ttcttctttc aattttgtca ttttctttaa aacaactaaa
    20041 aaaagagttc aagacaataa tccgtcctaa actccttctt cctatcttac aaaaagtatg
    20101 tcacttacgg tctttcatgt cgcctgtatt gataacaggt tgcgttcctt tgtctgtaat
    20161 gattgagaca agtaaattat tgatcaattg gactttccga tcatcggtga aattgatttc
    20221 ttgtccatct tcgatttgct ctaaggccat ttgagtgatt gtgacagctc cttcaacaat
    20281 ggtttgtcga gcagacaaaa tggcttttgc ttgttgacgt tggagcatgg cactagcgat
    20341 ttctgttgca taggctaaat ggttcaagcg tgtttcgatt acttcgacac ctgctactgc
    20401 taaacgctct tgcagctctt tttgcaactc gtcagacact gccgtcgtgt ttcctcgaag
    20461 cgtcaaatca ttctcattga atgtatcata cgggtattgg ctagcgatat gccgaatggc
    20521 tgtctcactt tggatttcta caaaatcttg ataatacgct acatcgaata atgcctttgc
    20581 tgtatcaacg actttaaaga ctactactgc tgagatttcg atggggtttc catccaaatc
    20641 attgactttt aaaacagaac tattgaaatt ccgaactttc aacgaaacag tcattttttg
    20701 tgccaaaggt atcgtaagga aaaagccatt ttctcgaatc gtacctaaat attgtccaaa
    20761 aaacaaaatt acttttgctt gattaggact gactacagtt gccgaactta acaacaataa
    20821 cgtgatcaac caaagaaaaa tacttaaaaa aagtgcccaa actttcgctt gccacatacc
    20881 taaatagaag aaaaacaatc caactaaagc taatacgacc aatccgacaa tccctgcata
    20941 accattgata taaaatgctc ttttttctct catgctactt cctgcgctcc tttatccttc
    21001 aataaatatc taatgttact tttttaattt ttttttcgaa tacgcgccat tacaccttct
    21061 agttctaact ctttgtcttc agtgatgtac caaccgcttg cctgtgtccc tccggctgcc
    21121 tgcggtaaat aatccaattt tgttcgaacc cagtgctttt cttctgtaaa atagtcttct
    21181 accaagacct ctagatccat ccctgctgaa agaggcaaat cttcccgaac agatttaaag
    21241 ataaatcgtt ccttcttccc atcatacttc aatattcctg tatcgataat cggtaaagaa
    21301 taataatcca gctctttctt caccgtcaat aaatgtttga aaacttcatg gtacacttgc
    21361 tctaattggc gaccttctga attgttcggg taattaacca tcactttttc tttgtctgga
    21421 tcatagaagg tttggcttag ttgagtgata taagtatgta atctttccat ttctgttttt
    21481 aacttttctc tttgaccttc ttctaccttc atatactctt tcaccctgtt ttctttttag
    21541 atattttctt tcttatttta ctatagttat gtgcgttaag cgatgaaaaa cagcaaagat
    21601 tggaaggatc tttgctgttt tttcttaaaa tatccagttg agtacaggat caatctgttt
    21661 cattgtcctc ttgatccgtc gtaatattga tcgatgtgtc tttagcagtg atctttagct
    21721 ttttctgtcc ttgttctaac aaagtatttg ttattggctg gtagtctttt tcaatataag
    21781 attcaccaga cgtttggata gtatagccac tgttttccat ctcatgcaaa gataaattgc
    21841 caccgtttag gatgactttt ccttctttgc tgatcaaact gtcttctaga tggaagttgc
    21901 tgttttcacc tgtgacgatc aagttatcca cattggcctc atctaatcga agtgagccta
    21961 attcatcttt cgcagttatt gttcctcctg taaattcatt gaacgatacc cagccatcat
    22021 gtgtcttagt attcactttt ttgattgata cttcgttgat atcaattgca ctattattcc
    22081 cttcgattgt caattcagat aattcatgat ctgcagggac aacaatagtg atttgtgcac
    22141 ttgtgtcttc taaactaatg attgcacctg ttgatttttt gactgcattc acagtcaaca
    22201 cgccattttt ctcctttatt tctggctttt ctgtcttatc ataagtcgtt tttatctcaa
    22261 aatccaatcc gcgtatgacc gaaactgttt gattttctgc atggatgacg attttgtttg
    22321 catccttgat tttttttgtc tcagtaacca tatcgatcaa ccgaggacca ttatcccaag
    22381 tcagactctt ttgagcgcct aatgtataag ttgcacctgc taggaggcca ccagtaagca
    22441 tcagaccaat accggtaatc aaaaaatgtt tcaatctcaa tttatgtgtc ctccttcaaa
    22501 cgcccgctga atgcagatct ctttttgtaa taaaacgtcg tcccatccat ttaacaaagt
    22561 tcatcatgac ataaaaaagc ccttttgttc ctttcagcaa cagtggcgaa ataaatactg
    22621 cgcttcctgt ggcgaacaga ccgacaccga taaagaataa tccactcaga atcgattgtc
    22681 caaacacact gattcctgag atgatcatga ataggccaac acctaagata ctgatacata
    22741 aaacatagat actaaaaatg agcgctagta aagcaaccaa tagtccccca ataaccaata
    22801 taaaccccag tgctagtgga atgaggacag gagaagcaaa taaaccgata atgattaacc
    22861 aaatcaggcg aatttgtctt tttggatttt tcactaactc gttttctgct tgttcttctt
    22921 ctgaaaagta actgattttt aattttaagg caaactgctt tggtgtacca tattcatcaa
    22981 tgatctgatc taatgttttt ccagaatcta aaaaatattc ctcataatac tggatcaaat
    23041 caaaacgatc gtcttcagga atttcttgaa gatgatccac aacttcattc aaatattcat
    23101 tcatttgtgc tctcctccaa taaccggtct aaagaatctc gaaaaacaat ccattcatca
    23161 ataatttctt ctaaatgacg acttcccatt ggtgtcaacg agtaatatcg acggtttctg
    23221 ccttcaaaag gttcatcata ggtctcaaga tatccttctt ttttcaatct tctcaaaacc
    23281 ggatatagtg tagattcact gacagagaga ctttcttgta cgatttttgt taaggcatac
    23341 ccatacatat cttccttgct taatgctgct agcacagtac catcaagaag aacagttgga
    23401 atctggatgt gcattacgtc accttctatt ctcaagtgta atattttatt tcaatctata
    23461 ttatacaaca tataatatag tttcaaaatc agtttgtcaa atacaaaaaa actgactaaa
    23521 agatttgatc ctttttagtc agcaaaaaat ggcttctctt tgatttttat tataacagtg
    23581 gcataatcca atttgtccac atccagctca gtggcatgat aacgatcatt gcaggtatca
    23641 tgtcagctac tgggaacatt ttcacgttga tcattctaaa accggtagcg accatcaaga
    23701 atccgccgca agctttaaaa tctaagatca tgtctggtgt agtcaatgga taaataaatt
    23761 ttgccaataa gaacaaaata taaaaaataa tgaattgggg aattgctatc acagaaacga
    23821 cataccctaa attacacgca aaaatagctg ctgtgaaaaa atcaaggaca gatttagaaa
    23881 tcaaaatcgt actgtctcct gtcatcccag aatctaaact tccatagatc cccgtaccac
    23941 ttgcacaaaa tagaacaatg accgtcacta atgtgctgat aaattcatcg tgggtcatac
    24001 caagcttttc actagggaag attttagaaa taggtacttg catcagcgca gcccccttat
    24061 ttatcccatt acctaaatga acaatcaaac caatcgctgt tcctatcact aaagcaaaaa
    24121 taacagccgg catatttttc atcggcgcaa tggaatagat tcccatcccc atcgaacaca
    24181 caccaaaaac catcgtcagc tgtgctttaa aatgttcact taatttattt cctactaatc
    24241 cgccaattag tccgccaaac aaaacggaac ctacattgat caaaactcca gttggcatca
    24301 aaaaactcct ctctttttct acgaagcgaa aaaaatcaga aaaatttctg taattattgc
    24361 tttcgctgca agaaagattg taacagacag agaagattac accaattcat tttttgtgtt
    24421 aaagtattct ttattggaat aagtaggagg aatatgatgg atatccataa attcgaagtt
    24481 tttcttgatt tagccgaaac aatgaatttc accaaaactg ctgatcgaca attcaccacc
    24541 caaggaaata tttctaaaca gattctttcg ttagaaacag aattagacgt caaattattc
    24601 gaacgcgccc atagaaaaat cgaattgacc gaagctggaa gcttattgct cccatatgta
    24661 aaaaatgtcg tagaacaata ccatgctatg caagaagtgc ttgaaaccta tacgaaagac
    24721 aacaatcttt cattaaatat cttaacgata ccgacaatga tcaattataa aggtttttcc
    24781 aaaatcaccg agtttttgaa acgccatcct gaatttacag tccagttgaa agaagtggaa
    24841 agtgtcgaat taacttacaa tgacgccaat gaacatagtg acacgatcca ctttgctaga
    24901 agtttccagg ccccaccaag ttcgattgag tggttgccta ctgaaacaga cgattttgta
    24961 gcagttctac ctaaaaatca tcctttagct agtaaaaaga aattggatct aagcgaactg
    25021 aaaaaggaga actttttgct ccttggcccc actacgaact tgtatcaacc tgtactagat
    25081 ctgtgtcgcg aagctggatt tgaaccaaaa atctcttata aaggagcaag aatcgacttg
    25141 atcatcaata tgattgcaaa tgacatgggg attgctattg ttatggaaaa aacagtcaag
    25201 aatctattaa aagaaaatac tgtgattctc cctatttctc caacaacaga gagttatctt
    25261 gcatttactc gtaaagtcgg tgagcattct ttggcttcag atacattttg ggagtattta
    25321 aatcaagaag aaaactaagc cctacatttt ctgtccatct atccacccaa aaaaagagag
    25381 cgacgcatga ctttcgctct ctttcttgca tcatgtcctt gtttcaatga cacctctaac
    25441 atttctcgta gttcaataag aaagcagcga tttttatgcc aaaagaaccg taagaacatt
    25501 tccctaatca caaaaaaacg actaaaaacc cctaaaaacc ttcaatagaa tttttagggg
    25561 ttttattctt tttcctattg tttacttaac cgtatcattg ctgtcgttcc tactccttcg
    25621 atactgctat aatcaatacg tccatcatgc agttctagaa tttttttaac cagtgaaagt
    25681 cctagcccat tcccagaaat ttgacgactt gtatctcctt gataaaattt ttcaaacatt
    25741 cgatctctgg tctcttcatt cattcctaca cctgaatcgg tcacttccag tacaatctct
    25801 gtggctgtct caaatagttt gatatgaatc tgtccattga tttgattgta ctttatggcg
    25861 ttatccatga tattcagcca cacttggtac aaaaactctt cattgcctgt gtaatttact
    25921 gcagcaagtt cgatatccaa ttctagctgt tctttctccc atttaggctg aaaaaaaacg
    25981 attacttctc tgatttgttc atctaatcga tactctttct tttccagttg gatacgctgg
    26041 ttttccagtt tgttcaattt taagatgttc tctgttaatt gtgaaagctg cgtgatactt
    26101 tcgataatcc gatataaaaa tatttggcgt tcttcgtcag agagatttgg ggcttgtaac
    26161 aattgaacat atccttggat tgttgctagt ggtgttttga attcatggga tacactggac
    26221 acaaagtcat tcctcagtgt ctcgatacta ttgagttctt ggaccataac gttgaaatct
    26281 ttatacagtt gctgtacttc tgctactttc tgctgttcat ccattcgaat cgaaaaatct
    26341 cccgttgcta ccagactcat atttgtccct agcgcactga ttggatgcag gatacgccgc
    26401 ccaacaaaaa tcgatattcc tgtacccacg accatactga accccgcaaa gacgaataac
    26461 gggtataacg ttggttttcc agccgattca gcaggcgaga tagaatgtcg agacaataag
    26521 aaaataaaac cgagaaaaca cagcagtgtc actaagatcg aaatgaagac aatactagca
    26581 aaatagatcc ataattgtga aaatagcttt tttttcatct ttaaataacc gccttatagc
    26641 ctagtccccg tactgtgatg atttgaaaat cttccgtctt ttcaaaacgg gtccttaaac
    26701 gtttgatatg tacatcgatc gttcgttctt ctgtattcgt atctaatccc caaatatcat
    26761 ccatcaactg ttggcgagtg aagatcttgt tgggataaga cagtaattta tacagaagca
    26821 gaaattcttt ttgaggcaga atcaacgaat gctgatcttg taacactgta taagcatcta
    26881 gctctaattt tgtttgtccg attatcaagc tactgctgta attgatcttt gaacgtcgaa
    26941 gcaatgcttc cacacgtagg atcatctcat tgacatcgat gggttttgtc atataatcat
    27001 ctgctcctaa tttgaacccg cgtttcttat cctcaaaatc ggcctttgcc gtaatcatta
    27061 gaacaggtgt gtcaaatctg gtgctacgta atgagtctag caagtcatag ccatccattt
    27121 tcggcatcat gatatctgta atgattaatt ctatacgtgt cgcttctagt tttttcagcg
    27181 catcctctcc attcaatgcg gtgatgactg aaaagcccgc tcgttccaac acagaacggt
    27241 aaagcaactg taattgcgga tcatcttcta ctactaaaat acgattcatt gatctccctc
    27301 tgttttttga aaaatttcta gattttttct tgtagttcat cttcagttaa catttatttc
    27361 gtatactacg atcattgttc tgatatactt attagtatac cgaatatttc aatacgggag
    27421 aagggtgatt atgcttcaat taaaagatat aaaaaaatat tataaagtcg gcgaaaccac
    27481 caccaaagca ttggatggtg tgtctgtcgc tttcagacaa aaagaattcg tagcgatttt
    27541 aggtcctagt ggttctggta aaacgactat gctgaatgtg attggtggac tagataatta
    27601 tgattccggt gatatggtaa tcaacggaaa gtctactaaa gactttaagg acagtgactg
    27661 ggatgcctac cgaaacaact ctatcggttt catttttcaa agttacaact tgattggtca
    27721 tctaggcatt attgaaaacg ttgaacttgg gatgacatta agtggtgttt ctaaagacga
    27781 aaaacgaaaa cgggcagaag atgcattgca ccgagtcggc ttgacaaatc atatgcataa
    27841 aaagccaaat cagttatctg gtggacaaat gcagcgagtg gccatcgctc gagcactagc
    27901 caatgaccca gatatccttt tgtgcgatga accaaccgga gcgttagata cagaaacaag
    27961 tatccagatc atggaattga tccaagagtt atcgaaagaa aaattagtga tcatggtcac
    28021 acataatcct gaactggccc atcaatacgc agatcgaatc attgaatttt ctgatggtaa
    28081 gatcttaaca gattcccatc ctcacatcga acgaccaaaa gatgatcaat ttaacttacg
    28141 cagaacaaag atgagttttt ggacagcgtt aaagctttcc ttcaacaata tccgaacaaa
    28201 aaaaggacgt actttcttga cctcctttgc ttccagtatt gggatcatcg gaatcgcgat
    28261 cgttctgtcc ctttcttctg gattccaaaa acaaatcgat aacacgcaag ctgaaacgat
    28321 ggcgaaattt ccgattacta tttctaaggt aacgactaac cagactcgtg acgatgccgg
    28381 attgggtgca agcaaagcgg attatccaga cagcaaaacg attactgcaa aagtcagcga
    28441 agaagataaa gcacagcaca cgaataagat tgatcaaaca tacgtcgatt acgtaacaga
    28501 catcgatcct aacttaagta ataatatcgg atttacccgt actacaggaa tcaatctttt
    28561 acgtgatgta aatggcaaag tacagcctgt cagcttctcg aatcaaaatc ctgatacgga
    28621 atctctttct ttatctagta cgatgtcagc aatgacaggt gtgggagtgt caagtttccc
    28681 tacacagctt gacactagca aagaaaactt tttaaaagac aattattctt tacttgccgg
    28741 aagttatcca gcctcagcaa cagatgtcgt gctgatcgta gacggcaata ataatactaa
    28801 tatcaacgca ttgaagaatc ttggatttga tgtgaaagaa gacgaaaaac ttgattttga
    28861 tgatatcgtt ggtacaacat tcaaattagt aaacaacaat acttactata caaaacttcc
    28921 aactgggaat tttatcccta atacagatta tgatgcgatg tatcagaatg cttctgatga
    28981 attgaagatc tcggggatcc tacgcgtgaa atcttcttct acaatgaatc tcttatctcc
    29041 aggaatcgct tatagcgatc aattaacgac tcaaatcgtc aacgaaaaca aagaatcaga
    29101 aatcgtcaaa gctcaaagag acagtggtgt aaatgtctta actactgaaa aagttgacga
    29161 aaatgccaaa caaacattgc ttagttattt aggtggagac agcttgcctt caagtatcat
    29221 gatttatccg aacaattttg aagacaaaga aaagatcttg gattatctag atgattacaa
    29281 taaaggcaaa tcagatgaag ataaaatcat ttatactgat ttagctggaa cgatgactga
    29341 actgactggc ggattgatgg atgctatcac ttatgtcctt attgcttttg cagggatctc
    29401 tttagtaaca agtatgatca tgatcagtat cattacttat acatctgtga ttgaacggac
    29461 aaaagaaatc ggtgtactaa aagctttagg tgcacgtaaa aaagacatca ctcgcgtatt
    29521 tgacgcagaa acgtgtattt taggtatttc ttctgggata ttaggtgtgt ttattgcatg
    29581 gctagctacg ttccctatca atagcatctt atacaacatg acggatctaa aaaatgttgc
    29641 tcaactaaat cctgtccatg cgattattct agtgatcgtc tctactatat tgacgatgct
    29701 tggcggtcat cttcctgcac gaatggcagc gaaaaaagac gccgctatcg ctttgcgtgc
    29761 agaataaggt tgtgacaacc acatttagat ttgagtcata agagcgctga aatagtagaa
    29821 aacagcaaga aacgaggaag taatatgttc cttgtttcgc gctgtttttt ttaattattt
    29881 tttataattc tggtatactt ttctttcctt tgtaccacaa ctcttctggc gtatacctgt
    29941 cttttccatt tatgaatact gttccatctg cttcaatcgt tacttcagtc ccaatccaca
    30001 tattacgaga aagtgcgaga agattttttc tttttcctct tccttttttt ccaaattgca
    30061 aggcatcggt tatctcttct gtccaatgag atttcacgta ttttacttct ccgttttcaa
    30121 atgtaacttt caaaatattc gctatatttt ccacttttac tgctttagga aatgactcct
    30181 ccattttttt cactttcctt cctccaacac taagtatgcg ctttctttta gtgataagta
    30241 tatcatagtt tattctatta tcaaaaaaac gcatccattc cattgtagaa tactttaccg
    30301 aacaagttga attgttttcc tctttttcac cttttactat agataggtaa agacaactaa
    30361 ggaggaatct ctcatgacag atcaaccgta tgatttaaga aaagtcatcg aagaactaaa
    30421 aacacttcca ggtcaatatc acgaaacaga tattgaaatc gatcctgaag cagaattatc
    30481 tggtgtttac cgttatatcg gtgctggcgg tacagtaaaa cgtccgactc aagaaggacc
    30541 agctatgact ttcaacaata tcaaaggttt ccccggcaca cgtgtgaaca tcggaacaat
    30601 ggctagtcgt aaacgtgtag gtgaaatttt gcaccatgat tatcgaacac ttggtcgttt
    30661 cttgaaagac gctgtagaaa atccaatcaa acctgtgaaa gtagacaaag cagacgctcc
    30721 agcacaagaa gtcgttcatc tagctaccga tgcagacttc gatattcgca acattttgcc
    30781 tgcacctaca aatactcctg aagatgctgg tccatacatc acaatgggtg tagtcttagg
    30841 atcagatcca gaaaaaacaa tgagcgatgt aacgatccat cgtatggttc tagaagataa
    30901 agatacgatc ggtatgtaca tcatgcctgg cggacgccac attggtcact tccaaaaaca
    30961 attcgaagcc ttgaataaac caatgcctat cactatcaat attggtttag atccagcaat
    31021 tgccattggc acgactttcg aaccacctac cactccactt ggctacaatg aactatggtg
    31081 tgcaggagct ttgcgtaacg aacctattca aatcgttgat ggtatagcag tcgatgaagt
    31141 gggtattgct cgtgctgaat tcatcattga agcagaaatc atgccaaatg aaacaatgca
    31201 agaagatatc aacacgaact caggaaaagc aatgccagaa tttcctggct atactgggga
    31261 tgctaatcca gctgtacaag tagtgaaagt caaagcagtt actcaccgaa aagacaatcc
    31321 aatcatgcaa acaacgattg gtcccagtga agaacacgta tctatggccg gtattcctac
    31381 agaagcaagt attcttgcct tggtcgataa agctattcca ggaaaagtag taaatgtcta
    31441 caatcctcct gcaggtggtg gtaaattgat gaccatcatg caaatccgta aagaaaacga
    31501 agcggatgaa ggtattcagc gtcaagcagc tattcttgct ttatcagcct tcaaagaatt
    31561 gaagacagtc attttagtcg atgaagatgt tgatattttc gacatgaacg atgtagtttg
    31621 gactttcaac actcgtttcc aaggagataa agatatcatc gtgatcccag gaatgaggaa
    31681 ccatccattg gacccatcag aattaccaga atataatcct ggctctattc gtttccgtgg
    31741 gataagtgca aaaactatct ttgacggtac agttcctttc gatatgaaag aacattttgt
    31801 acgtgcaaaa ttccaagaag tagaagattg ggaaaaatat ttgaaataat gaatgatctg
    31861 caactgtttt gacatcataa aaaggagtgc ggaaaatgga acaattggca caacggaaga
    31921 aaaaaatcgt gattggtgta agtggcgctt caggcaccat ttatgccatc aacctagtga
    31981 agaaactaaa agaatatccg atgatagaaa cacacggaat catcagtcca tgggcaaaac
    32041 agaacttaaa gctggaatcc gacttatcac taactgaatt cgaaagcctg tttgattacc
    32101 attattcaaa caaagattta ggtgctgctg tcgccagcgg atcatttttg accgatggaa
    32161 tgatcatcgt accggcaagt atgaaaacag tcgcaggtat ctctgttggt ttaggcgaca
    32221 acctgattgc acgtgctgct gatgtaacaa tgaaggagca acgaaagtta gtcattgttc
    32281 ctcgtgaaac gccattaaat gccattcact tggaaaactt gactaaatta gcacgactag
    32341 gtgtgcaaat cataccacca attcctgctt tttataatca gcctcaaacg atccaagatc
    32401 ttgttaatca ccatacaatg aaacttttgg atcaatttga aattgtaaat gagcattcaa
    32461 aacgttggca gggtgattaa tatggaaaaa gaaacgatag ttgaagtaac caaactaggt
    32521 ttcacgatcc aagctgctgt taaagtaatc ggtaaagact tattgatcac tttaacaggc
    32581 ggagatacac cacatatcgg aacagtcact acattttctc gtgatacaga gatgcaaact
    32641 gtacgtttcc ctagccacga tggtcggttc cataaagacg atgtcttagc cgaaaaaata
    32701 gctgtgatca tcaaaactgc cgttccggga agttgtacga tcacttccgg tatccatgtt
    32761 gaccatatct ccaatgcaca aatcaacgcc tcttttccga tgtcagaaga gttaggttac
    32821 aagattttag attggttgga aaaaatagat ttccatatca aataaccagt ttattataaa
    32881 gatggtgaaa aaccattatg aaagaagagg ttgtgaaatc agcgttatga cctgagtatg
    32941 agagaaaaaa atggaaaaat agcttctcat attttgccgt ttttgagcta ataccgaggg
    33001 acagctgatt gaacgccgtt tatcaagagg ttgtgataaa gccgcttcgt ttcgagtagt
    33061 aagaaaaatt ttgtaaacat gggtttccac atttttgcat aaatttgact tactaccgag
    33121 aaactggctt ttgaacaccg tttatcaaga ggttgtgaca cgtcttttgt cacaacctct
    33181 tttatttttg cttcactgta actgctacaa tatcaccgat ttcttttccg atgtttttac
    33241 gaatatcttt tcgtagtcca aggatgtagc agactgaacc atctggattt ttcacgccca
    33301 tattcacgat actgccttca tacggcacat tgtcaaaagt tgcttggact ttaactcttc
    33361 cagcaccgaa ttcttttctt atatcatacg gaaaaataat atatgccccg cccttgccga
    33421 cttcggaagc atagattttt gattcaaaag aataaatttc cctcatttta tttctcctat
    33481 tttttacatc tagtatagca tagcaaaaaa aagaaggaac tgtgacaaaa gtcttgtcgc
    33541 agttcctttt ttcgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    33601 gtcaacaaac atgagaagct gttttttgaa attgctcctt gtgactcaga gttaaacggc
    33661 aggctcacaa cctcttcctt aaacggtgtt caaaagcttg catctacggc actaagccca
    33721 atcagatgaa aaatatggag tgctattttt atctgattgt tcttactgct tgatgcagag
    33781 cagctttttc acaacctcct acatggtgtt caaaatatta ttcgcctttt gcttctttca
    33841 ctaattttgc gaatgcttgt tgttggaatt cagcagctaa ggctttcgta cgttgttctt
    33901 gctctgcttt gctcattttt ggattttcag tcattgcttt taccataccg tcttgaacat
    33961 aagcttttgc ttcactttcc atcgtacttt gctgtttctt cactaattca gctaacttgg
    34021 ctgcttggtc cgtacttaat tctacccatg tctctgggat tttgaatgta ttcactcggc
    34081 tgtcatattc gtgattgtta tcagcaatat tgaaccagaa tttatacatg tcgtttttgt
    34141 attcccaata agtagttttc gtcacttttt cagcatcgcc gtctgtcttt tctactttat
    34201 ttgtttcgtt atccgtacca gttgcttttg gtttttcttg tttttcattt gttcggtaaa
    34261 gatacacttt ttcttttcca ttccctaaag ctttatacaa aagcatatct gctccttggg
    34321 aatcagcact ggaaacaatt ttttctgttt ttgtttccgt tactttttcc attccaaagt
    34381 gattggacaa attagcgacg atcaaactta gtgaagcgac gaagattagt ccgaaaatca
    34441 aagataaaaa ggtttgccat gtttttttag cgaacacgtt cgttaaagca aaagcaagca
    34501 agctgacaat caaaactcca ataatcatgc attatccccc gctttcatta gaacaggttc
    34561 ttttttcttc aataaaaagg caataataaa gccaagaatc ccaaagatga tcgctacgat
    34621 aaacgcggca tggtaacctg acagtgtggc attgattgcc tgatctttat aagctaatgg
    34681 cgtattatcc aatacagatt tgcttgggat atttgctttt gtcacattcg tcaacacact
    34741 gatcaaaata gctgtaccaa tcgagcttgc gatttgacgg aatgtattat ttaccgctgt
    34801 cccatgactg atcaagttat ttggcaatgc attcatacca gaagtcgtta ccggcatcat
    34861 gaccatggag atcccaaaca tacggatcgc ataaaggatc acgatatata aaactggagt
    34921 cgtttttgtc aagaaagcaa atggtgcagt tgccgctgtc aaaatgaaca tccctgtgat
    34981 ggctaaacgt ttcgcgccgt atttatcgaa aatcgcaccc gtcacaggca tcatagcccc
    35041 catgatcaat gcacccggca acaacatcaa gccagaatgg aatgctgatt ctccacgaat
    35101 attttgaata tacaatggta cgaccatttc tgcccctacc atcgccatat ttgttacacc
    35161 agctaaaaca gctgcaatcg tgaatgtaga tgatttgaat acacgaagtt ccaagaatgg
    35221 atgttccaaa tgtaattgac gccatgagaa caaggcgatg aaaatcacac caacaactaa
    35281 gaaaccaatt actttcaagc ttccccagcc gtcatctcct acactagaaa atccgtaaag
    35341 taaactaccg aatccaatcg ttgataaaat agcggataaa atatcaatgc taggatttga
    35401 caatggaata acacttttca ttaggaagaa tgctaaaacc aagacaagaa caacgattgg
    35461 cagtaccata ccaaacaagt cacgccaatg gtagttatca atgatataac cagataatgt
    35521 agggcctaaa gcaggagcaa gtccaataac gatccctgac atccccattg ctgatcctct
    35581 tttttctggc gggaaaatag atagcatgat attttgcaat aatggcatcg atactcccac
    35641 accgcaagct tggatcaaac gaccgattag taatgtacta aaattaggtg ctgtaaagca
    35701 agtaatcgtt ccgatcaaaa agatcgtcat ggcagaaaga tataaatttc ttgaactgaa
    35761 tttattgatc agccatgctg ttattgggat catgatcccg ttgactagca agaaaccagt
    35821 cgttaaccat tgcacatcag acgctgaaat atcgaaagct ttcatcaatg taggaaaagc
    35881 tgtcgtcaat aaggtctgat tcaaaacggt acaaaatgta ccaattaata agacaacaac
    35941 taaaagtgtc cgattatatg gttttccgta aatatctacg ggttggttgt tactcatatt
    36001 tctccttctt tctgaatttt ttctgaaact actagaatac tttagtccta aattcgctat
    36061 ttgtcaacct acttgacaca taagttaata cggatataat taagggaagc aagaggacaa
    36121 aaggagtgac aaaaatggtt ggggcttccc ttaaacgttt gttggaaaat gatcaattgg
    36181 ttgttggcat cagtgaatta agtgatatcg taggtgtgtc tccacgtcaa ttaaggtatt
    36241 gggaacaaaa gggattcatt cgttcgatcg aagctgatgc gaattgtcct agaaaatatc
    36301 gtttacctac agtcataaaa gtagaactga tcaaaaaata tttagatgac ggatttactt
    36361 tgtcaaaagc tgcagaaaag gcagaaatcc gccaaaagaa aatgcaccat gtccgtaaag
    36421 ttttttcaaa agcaatcaaa gatatcgaat tgatcgacga acgctatacg attttacaaa
    36481 tcggtaactt caaagaagag aatgctaaga tgtacatcat tcatgacaac gagacagacg
    36541 aattaactta caagctcctt cctgtcgatg aaaaagctga ctacgaacag ttgttaaaag
    36601 aactctaaac gccactaatc tcctaagtac caagcttttt aataagtaga tgctacataa
    36661 atacttgaaa aaaggcctgt ttgaacataa gaatatccga acggtgatct gcgtttcttt
    36721 catatagaaa ggaattctcc atcactgttc ggatattttt ttattttttt cggttacttt
    36781 cttctaatac atctgttagc caatttgcat attctgtttc ccgattgatc gcatgcgcta
    36841 aaatcaggta gtgtccgtaa ttttcatgtt tttctttttc agtaggaaag atcgtttttt
    36901 ttcgctctaa tagatgaagt cgtttttctt catgaaggtc acgctgctgc tgaataattt
    36961 ctactaatgc cggatcattc acgtctttga cgaaatacaa tttcagaaca aattcgtctc
    37021 gattaaccgg tagttcattg gtgggtgtat gaatccactc tgccaattgt tccttccctt
    37081 cttctgttaa ttcataaact ttcttttcca atttttcacc tgtgatctct acttgatgct
    37141 gaatgattcc ttgctcttcc atttttgcaa gttcaggata aatctgacta tgttttgctt
    37201 gccaaaattc tcctatttca tttttgaacg attgattcaa ttcatatcct gtcatcttct
    37261 tcttacttaa taaccctaac aaaatatatc gaagtgtatt ttttcttgcc atcagttttc
    37321 cctccttgac aaatctttaa atccattcta tcataaatga gtaatcgaaa acatgtagtt
    37381 tattacatgt tttaatttat aaggagatgc ttaaacatga aagaatttaa agaattggaa
    37441 gattttattg gaactcactt tatctatacg tatgacaatg gttgggaata tgaatggtat
    37501 gccaaaaatg accatactgt tgattaccgt atccatggcg gaatggtcaa aggccgttgg
    37561 gtaaaagacc aagaagctga tatcgttaaa ttgacggacg gcgtattcaa aatcacttgg
    37621 acagagccaa cagggacaga cgtggcattg gactttatgc caaatgaaaa gaaacttcac
    37681 ggaatgatct ttttccctaa atgggttgaa gaacatccag aaatcactgt tactttccaa
    37741 aacgaacata tcgcagaaat ggaagcagct cgtgaaaaat atgaaactta tccaaaatta
    37801 cttgttcctg aatttgctac gatcacttat atgggtgatg caggtataga caatgaagat
    37861 gttattagcg aagaaccata cgatggaatg ccagatgata tccgtagcgg aaaatacttc
    37921 gatgaaaatt ataaacgagt aaaataaggt tgtgaaagag aggttgtgaa atcagcgttc
    37981 tgacctgagt attagagaaa gaaacggaaa aatagttcct catgttttgc cgttttttga
    38041 gctaataccg agggtcagct gattgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38101 atgctttgag taataagaac gctagttaga aaatagcttt tcatattttc taggctagtg
    38161 ggcttattac cgagaagcag ccgtttgaac accgtttatc aagaggttgt gaaatcagcg
    38221 ttttgacctg agtattagag aaacaaatgg aaaaatagtt cctcatattt tgccgttttt
    38281 tgagctaata ccgagggtca gctgattgaa caccgtttat caaagattgt aacaactgca
    38341 tattcacaca gttaaattag tgacttcaaa atgaaataat gctcccagca aaaaattagg
    38401 ctgggagcat tatttcgtta ttctgttcat caaaaatcag cgttttgatt ccatttcatc
    38461 acaagttcct cctcatttac tgcttcatct ttttgctgtg tttgaattgt aaaaccttgt
    38521 gaaagataga aatgaatagc tgattgattt ttttgataga ctgtcaaagt cagtgtttta
    38581 ttggtttctt ttattttttt cattagttgc gtaccgattc tttgttgccg gtattccctt
    38641 ttcacaaaga ttcctgcgat aaactgatca atcaccccag caaaaccaat aatttcttga
    38701 tctatttctg ctaagtaaat cactgcatct ggtaaagctt tcctaacaga ttctacttga
    38761 ttctcccaat aagttgatgg aatgaattca tgagaatcca agttagcatg cagccaaatc
    38821 gatagaacag attccagttc tttggcagac aattttgttg ttgtgcggat cattttttct
    38881 ctccttactt attcattgat tttaaaataa aaaccatttt tttgaagctc ttttacaagt
    38941 tttcgcccct caaaaaagta tatcttctct tgttttactt ttctaatacc aaaagaaaca
    39001 gcaactttag gatcatcagc tgaaacgatt cgataagttc caccaggcaa ttgatccagc
    39061 ttgactttcc catgatggtc gctttgtact gaaaacggca caccatttct agtaagtgtt
    39121 tttttcttat gtttcaaaat gaatgaacta ccatttaccg gtttccctga cgaatcacta
    39181 ataataaatg aaaaatcaag cactttttta cttaatctat actgatagat tagtcgtccg
    39241 ataaacacta acagtatgac tacagctatt atcgttcctg caagaataaa ccattgccgc
    39301 atggttcttt cctgatttcc ttttccttct tcttttttta ccgattcgtt ataaggaatc
    39361 cgatgtcctg tcactagcaa acgatgcgtg ttgatcatat acggcgtaca tgtaagcaag
    39421 gtaactaagt cttctccttc tgaaatcgtt agaacagacg tgtcatctgg cagtaccact
    39481 tgtatatttt taacttgata agccaatttc tcccccaaaa cagtcaaaat aaacgtgtct
    39541 ccgttttcta aacgatctaa ttcggtaaat aaccgtcgat taggtaaacc gctgtgagca
    39601 gagatcactg aatgcgtcga atttccacca agcgggaaag aggttccttg caagacgcct
    39661 gctccatttt caaggatcct accattgagc cgatcataaa gtgaaatatt taattggatc
    39721 ttaggtatat tgatacttcc gatcagccat tcctctaatt ggctggatgt tgtactttca
    39781 tttctgttct tttcattgaa aggatcagta ccaggattga atccttggtc agttaatcgt
    39841 tggtttttct cctccatttt ctttcgctgc acttcaattt cagctgcggt ccgtttttgt
    39901 acttgttcga tacgtttttg atcaataaaa ctatttaaag aatccacata aaatggatat
    39961 aaagcgatca gtaaccctac gacgatacat attaataaaa acaaacgaaa caaaagtcga
    40021 cgagactgtt tttgcttact ctgcttcaca tgtgtcctcc cttgcaagtt tttttgttct
    40081 ttcttttatt atatgggttc aggaaaaaaa tagaaaatga aagccgatga ggttgtgaaa
    40141 aagctgtcct gcatcaagta ataagtacag cccaaaaaaa tagctactca tattttttgt
    40201 tgggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    40261 aaaactgcgt tatgctttga gtaataagag cgctagtcag aaaatagctt ctcatatttt
    40321 ctaggctagt gagcttatta ccgagaagca gctgattgaa caccgtttac cgagaggtca
    40381 tgaaaaagct actttgtttc gagtattaag aaaaattctg tttcaaaaca aggagatgca
    40441 acaaaactta tgtcgcatcc tctctcctta gaactttcac ttatgttttt cttgtaaaat
    40501 tcataaatat tttcatgaat tttctaattt cttgcctacc tctcgcatag gatatatgat
    40561 gaatttatct aaagtttgca aaggagttcg acaattgaaa aaatatggcc catttttagc
    40621 aatttttttc attgctttgg catttctcat gcctcattta ttcacacata aactaatttt
    40681 gggtgctgat gccccattcc attacaatcg gttctatgat gccgcgcaac aaattaaata
    40741 tggggatttc cattattttc cttcccttta tggattccag caaagtggca ggatcgtgaa
    40801 tgccgtttac ggcccatttt tcgcttattt acaaggattg atcgtgttgc ttgcaggtag
    40861 ctggtttcat tatcagattc tttcaaattt cattctttat cttatttcag gattttctat
    40921 gctgatttta ctgagatatg tcaatgttcg aaattgggta agtgtaccag tagccgtttt
    40981 attcatgagt acgtattcta ttcaatactg gtctatcaac caaggattca ctagctgggg
    41041 gacttgtttt tatcctttat gtatgatccc tattgctgat ttcgtccttc ataaaaagtt
    41101 ccctgttatc aaagtcgctg tctcagtggg acttatgaca cagatccatt tactgtcttc
    41161 gatcatgtta gctttgatgt atatcccttt ttatctttat tactttttta accagactga
    41221 aaaaggaaaa gcactgcttc aactgattaa gtcggtcttg ctttattttg ttctgacagc
    41281 aaatatttgg gcaggattag ctattgttta cagtgggaat aagattcttc ctccctttgt
    41341 taacagagaa atgagtcaaa aggcgataga tctggatggc agttattggc tccattaccc
    41401 taaggccttt cctatattga tattatcagg ttttttgctt tttattttct ttcaacaaaa
    41461 gaacacctta ttccaaaaaa tcgtgttgta cacaagtctt gttttctttt ttctttctac
    41521 tagcttgatt ccatggacga cattgatcga aaaaaatgtg ccttttatta gtttgatcca
    41581 gtttccattt cgatttttcg tgccatttac gctattgctt ctactttacc ttgccctttc
    41641 actgaaccag tggtctgaac aaaagtggtt ccgtctttta agtatatttg gcattcttat
    41701 tttttcagca cagacgatcc agaacttata ccagcatctg gaaaagtggg aaaatgaaac
    41761 atttgtcagt cgacatactt atctattcga tacgccggaa gaagcaagga aaacattctt
    41821 cagcaaagat atgtctacca gcttgttcgt tttccaaaaa accacacctg actatctgcc
    41881 aatctataag gagacaaaag acaataaata cgaccggtat aacgaagaaa tactagaaaa
    41941 cgaaacaact tttataaaaa cacatccagg cggtcttttg acaatcaagt ggaaaaacac
    42001 tggggacaaa actgttcata tccctgttat cgtctacgat cggactgtgc tacagcaaaa
    42061 tggaaaaaca ttgactgatt atgaagtaac agatatcggg accccaatcg tgaagcagca
    42121 aaaaggaatc aacgagctca cgcttcatta tcaaacgcca atctatttct atttcattct
    42181 ttctcttact ttgattggct ggtttacctt gctatgttta tttatttatc atagatacaa
    42241 gcttttacgt gcttaggcaa aagggataaa tacatccgat caaaaaactc tccaaacggt
    42301 ataatcgttt ggagagtttt attttaatca gtttttttat gttatatgtt atatttttta
    42361 ttcgttcaca tctaattcga tttccacccc gtagtgatca ctgatgattg gactatcgat
    42421 cccatcaaaa atccgttgat attttttaac ctgcattcct tcagggacga aaataaaatc
    42481 tatacgcaat ttttctgtat tttgttccca accatctatt ttcttttcca cagtagctgc
    42541 ttttcctttt tcattcgcga caacatacga atcttgaaga agaaggcaac tttctcttac
    42601 agtataataa ccttgagagt ctactggatt attgaaatca cctaggaaaa aaagtggtac
    42661 gcgagtttcc aaaaagtagt tttccaagtt tttccactca aaataaaagc cacttttttt
    42721 atctgtccac caagaaaagt ggcagctcga gacaaaaatg tcctgatcat ctatctttgt
    42781 tttcccaatc aagatcttgc gtgtctgata atcttctttc ttttgaaatt gggagacggt
    42841 aaggacctga ctttccaatg tttttttcgt aagcagtgca tttccttctt catatttatc
    42901 ataaccgatg tggctcattt cccaactcca aaaatactct tttccgtgtt cctttaaaag
    42961 ctgaactaaa cggtaggcaa aattatcttt acggataggt acttgattct ttaccggaca
    43021 gaacttcatt agcttcgcag gttctacttc ttcagattcc agtaattggt tgatttcttg
    43081 taaagcaatc acttcataat cttctaacag gatcttatct gctaattctt gcaatttttt
    43141 ctctggttct ggctcaagcc agctatgcgt gttgagtgtc atgaatttca ttggttttct
    43201 ccttgtcttc atttcgaaac aaaagggttg tgacaaaatt tttgtcacaa cctctccagc
    43261 aaatagtgtt ctcctgctaa ctcctcagta ataagctcaa tggaccagga gctatttttt
    43321 gtttggctgt ttttatcact tggtgcaggg cagctttttc acgacctctc cactaaacag
    43381 tattcaaact gctgcttctc ggtaataagc ccaaccaaac gaaaaatagg aggagctatt
    43441 tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct ctccttacat
    43501 tttaatggag ccgatcactt taccagctgt cactgtacct aattgcttga tcactacttc
    43561 ttctacttta tcgtttgtaa atacgatcat gatcgttgga tctttttctt cgttttggat
    43621 cacatcaaga tccatctcag ctaatttcgt tcctgttcct acttcttggc cttctttgac
    43681 agcaatctca aaagctggct gattcatttg gactgtatcg attcccatat ggatcaaaac
    43741 ttcgatacca tctttcgttt ccagcccaat tgcatgtttg ctagggaaaa tacttgtcac
    43801 ttttccagcg atcggcgcaa caacttctct tgatgttgga ataacagcaa agccgtcacc
    43861 catcattttt tgtgagaata ccggatcttt tacttcagat aaaggaatca cttctccaga
    43921 agctgcggaa actagttctt gttctatacc taagtattgc ttactacctg cttgtccatt
    43981 tcctgtagaa accatttctg gctctggaat ctctgctcct gattgcagca gatcttcgat
    44041 atctgatttc aagacatctg cttttggtcc ataaactgct tggactccat tgtctttgac
    44101 gatcaatccc atcgcccctg ctcgtttcca ggcttcttca gtccctactt tttctcgatc
    44161 atttacactt acgcgtaacc gagtcataca agcatcgaca tctttgatat tttcttttcc
    44221 tcccagcaaa tgaatgatct ggaccacttg ctgatctgct gttcctgttc cgctgttaga
    44281 tacaacggtt tcttcactgt cattgtcgta atttccgtta cgtcccggtg tagcaaagtt
    44341 gaattttttg atcaagaaat ttgctaagaa atacgtaaca acaccaaaag cgatcacaca
    44401 caacacgaag ttgataagat ctccacctag acccgctttg atagctaacg gtgtccgagt
    44461 cagcaattcg atattcccaa atgaatggac ccgcaatggc aagatatctg ccatcgcaaa
    44521 tgcagcccct tggatcactg aataaatgac atataatggc acagcagcaa acataaacat
    44581 gaactccaat ggttctgtta cacctgttag aaagacagcc aacgctgctg aaaaatacat
    44641 tgatttatat cttgctttct tatcaggatc gacattacga tacatcgcaa aagccattcc
    44701 catcaagata cctgaagaac caatcatttg tcctactttg aaacgagccg gcgtccagtt
    44761 atgcaacaca aactcatatt gcgacatatc ccctgcacct ttaagattca ctaaatcagt
    44821 ggcccaagcc aaccataacg gatcttgccc aaatacttgt gtccccgcct gtgcgcctga
    44881 taaaatctca tacgatccgc caagctgtgt atagttgata ggaatcgtca gcatatgatg
    44941 caatccaaat ggcaatagta aacgttctaa tgttccaaat aaaaacggtg ctagaatcgg
    45001 tgcgctttct tgtgattctg caatccataa accaaaatta ttgattccag cctgtacgtt
    45061 tggccacacc acagcaagaa tcaatgccac gattgttgac cataagataa caacaaacgg
    45121 aacaaagcgc tttccattga aaaatgacaa agcatccggc aattttctat aattatagta
    45181 cttattataa gccattgccc cgacaaatcc agcgatgatc cctacgaata ctcccatatt
    45241 caaagctggt gcttccaata cacttgtaaa aaatccttta accatgatct tcgtaccgaa
    45301 cagagtgtgg gtaaatgcat cttcattcgc aagcatgtca cttgtgacac caaagatagc
    45361 cccagtgatc cgattgatta aaataaatga gatcccagct gcaaatgctc cgcctgctct
    45421 ttcttttgcc caacttcctc ctattgctaa agcaaataaa atatgaagat taccaataat
    45481 tgcccaacca atattttcaa ttactccgcc cgttgtgaca agcggcgtcc aatcaggatt
    45541 gatcaaggga attgatttcc ctatactgat catcaaccca gctgccggca taactgctac
    45601 cacaaccatt aaagcctttc caaatttttg ccaaaactca aaactaaata tctttttcat
    45661 tccgtttagc tcctttacgc aatcggtttc gttaaacgta ttatagcgtg cgttttcttt
    45721 tttgtaaatg ctttattttt tatttttctg tctataccat tctataaaac ctataatacg
    45781 tgtgttccct tctataattt tctcctaaaa atttattttt ttaagctttt ttctatgaaa
    45841 tattttcttt tgtctattga cttgtgcaat cggttgcgtt tattatgaac gtataaaaca
    45901 gctcattagg aggagaaaaa tgaaacagat caaacgacta tttgctattg atccatggaa
    45961 aatcagcacg acacaattag ataaaaaaaa tcttcgactg caagaatctt taacaagtat
    46021 tggaaacggt tatatgggga tgcgcgggaa ctttgaagaa acttactcag gggaccacca
    46081 tcaaggtact tatttggcgg gtgtctggta tcccgataag acacgtgtcg gctggtggaa
    46141 aaatggttat ccagaatact ttggaaaagt gatcaacgcc ttgaacttta tcgcgataga
    46201 tatttcgatc aacgatcagc tgatcgactt ggcttctttg gaaccagaag acttttactg
    46261 ggaattagat atgaaaaatg gtgtattatc tcgtacattc acaatcacta cttctactaa
    46321 taaagtacgc atttccttcg aacgttttct gagcattgtc aaaaaagaat cagcttatat
    46381 ctatttaact atagaaatgc ttgaaggtag cggaacagtc aaactcgtat caaaattgga
    46441 tggaaatgtt caaaacgaag acagcaacta tgatgaacat ttttggaatg agattgatcg
    46501 tggaactata gaagaaatcg gctatttgac gactaaaact attccaaaca actttgggat
    46561 tgaacaattt actgttactg cagcaatgcg ccatttcgtt aatggaaaat cagtcatccc
    46621 tgactatcac gaagatactt tagcattaca tggttgcttg tctgcagaat tatctcaagg
    46681 tgagacatgg tcagtggaaa aagaaattat cgttcttact agtagagata ttccagaagc
    46741 gcagcaagca gaaaaagctg tctcagaatt aacgaaccag acaaacaatt atatccaagc
    46801 aaaagaagag caaacagcgg cttgggcaga acgatggaag cttgccgatg tggtcatcga
    46861 aggagacgaa gaagcacaac aaggtattcg attcaatttg ttccaactat tctctactta
    46921 ctacggcgaa gatgaacgat tgaatatcgg ccccaaagga ttcacaggag aaaaatacgg
    46981 cggagccact tactgggata cagaagcgta tgctgtccct ctttatcttg ctttagccaa
    47041 acctgaagta actaagaatc tgctgaaata tcggcataat caactgccgc aagcaatcca
    47101 caacgctcag caacaaggat taaaaggcgc gctttatcct atggtcacct tcactggtgt
    47161 cgaatgccat aacgagtggg aaatcacatt cgaggagatt caccgaaatg gcgcgattgc
    47221 ctatgcaatc tacaattata cgaattacac cggcgatacg gaatatttga aaaatgaagg
    47281 actggaagtt ttagcagaaa tcgcacgctt ctgggcagac cgtgtccatt tttcaaaacg
    47341 agcaaataaa tacatgatcc acggtgtgac cggcccaaac gaatatgaga acaatatcaa
    47401 taacaattgg tatacaaata cgatcgcagc ttgggtcttg cgttacacgc gagaaaatta
    47461 tttaacttat caaaaagaag ctgctatcac tatcagcgaa gaagaactag ccaaatggaa
    47521 agatatcgca gagaacatgt acttcccagt tgacgaagaa ttaggaatct ttgtccagca
    47581 tgacacattc ttagataaag atctcatgcc tgtatccgat attcccacaa gcgatcttcc
    47641 tctgaatcag cattggtctt gggacaaaat ccttagatct tgcttcatca agcaggcaga
    47701 tgtactacag gggatttatt tcttcaatga tacttttaca aaagaagaaa agcaaaaaaa
    47761 ctttgatttc tatgaaccaa tgactgtcca tgagtcttcc ctttcgccaa gtatccattc
    47821 gattttagca gcagaattag gaatggaaag caaagctgta gaaatgtacc agcgaacagc
    47881 tcgcttggat ttggataatt acaataacga cacagaagac ggcttgcaca tcacttccat
    47941 gactggtagc tggttgtcga ttgtccaagg atttgctcaa atgaaaactg cccatgatca
    48001 gttgagattc gcaccatttc tgccaaaaac ttggaaagct tattctttcc atatcaatta
    48061 tcgcggacgt ttattgttca tctcagtcac agatgaacaa gttactttcg agttattaga
    48121 aggttctcct ctagaaattt acgtctatga acagtcttat tctttagcgg atcgtctaga
    48181 aataacgcta agaaaggagg aagcaaatgt ttaaaggggt attgtttgat ttagacggtg
    48241 tcattaccga tactgctgaa ttccattatc gtgcttggcg aaagcttggt caggaaatcg
    48301 gcattacgat cgaccacaca ttcaacgaac agctaaaagg cgtcagccga gaagactcac
    48361 tctctttgct tttagcttat ggcggaaaag aacactcttt ttcaaaggaa gaatttgcag
    48421 aattagcgaa aagaaaaaac gattattatt tagagatgat ccagacgatc gaaccaaaag
    48481 atgtcttccc cggggtcgtg cctttactgg attctttaaa agaagaaaaa atcaagattg
    48541 ctcttgcttc tgcaagtaaa aacgggccat tcctacttga aaaaatgggt cttacacctt
    48601 actttgatgc tattgccgac ccggcagaag caacaaacgg aaaacctgcg ccagatatct
    48661 tcctccttgc agcaaaagct gtcggactaa aagcagaaga atgtatcggg atcgaagacg
    48721 cgcaagccgg tatccaagcc atcctctcaa gtggcgctca accaattggc gtgggacgtg
    48781 ctgaagacct tggagaggag atcccgcttg tccctgatac gacttttctt acgatcgatt
    48841 acttgaaaga aaagtggcat gaccatggat aatccagaga tctccgtcgt accagttcca
    48901 gagactgaaa taaagaaaat cacattgaag aacgactact tagaagtcgt tcttctcaat
    48961 tacggtgccc gcctgcacca aatcttcgca ccagacaaag aaggaaaatc agaaaatgtt
    49021 ttattgtctt acgatcgttt cgaagatgtt ctaacagaca aaagcttttt tggagcaact
    49081 gtcggacctg tagccggaag gatccgggat gcttcttggg gtgagcatag attggagaaa
    49141 aactgcggat cacaccatat ccacggtggc acgaaaggct ggtctttcca attttgggaa
    49201 gtagaagttt tcaaaactcc atcatcaata ggtgtggtct tttacttaaa agatgaattt
    49261 tctacctatc caggaccaat cactgctacg atcacttacc gtttaacaaa aaacgaatta
    49321 gagatgacaa cctctgctag tagccttgtt gaaaccatct gcaatccgac caaccatgcc
    49381 tacttcaacc tttcaggaaa tggaacaaga gacatctatg aacatcagtt aacggtcttt
    49441 ttagatggtg tgcttgaatt agatcaagaa aaactgccta ctggaaattg gacaaaaaaa
    49501 gaaaaactgc caatcgactt tagaaaatcc ccaacactgc aagagatcct tgcatgttat
    49561 ccagatggac tagatgatgt atttcttttg catcatccta ggctttccaa aacttcctta
    49621 caattattcg agaagcattc tggtcgccaa atgactatag caacttctaa taaaagtatg
    49681 gtattatttt ctacgactgg attcgaagct gatttctctg tcaatggaaa acagatgcat
    49741 tcaaactacg gattagcaat cgaaccgcaa gaaatccctg atatcgttca tttccccaaa
    49801 tttggatcga tcaacctcca tcctggtcaa gaacggatca gtcagacgat ttatcggttc
    49861 tctgcacaat aacaagagaa aggtgttttt atcatggcta ttaccgtaaa agatgtggcg
    49921 aaaaaagccg gtgttgccac ctcgaccgtc tctcgtgtca tcaacgatca tcctagtatt
    49981 tccgagacaa caaagaagaa agtccgaaaa attatggatg aactaggtta tgttcctaac
    50041 atgactgccc gcaatctagg aaaacgcatt tccagtgcca tcggcgtaat cttaccccct
    50101 cttgattcaa aagagcggat cggtaaccca ttttatcttg agatcatgga agcaatcaac
    50161 gaagaagcgc ggaattatga tatgactaca gcaatcgcta ctgctaaaaa ttttgaattg
    50221 ctgttagaga acgtccagcg tatgcattta caaaaacaag tagatggttt tattctagtg
    50281 tattcagata gtcaagatac tgtcattgat tatttatacc aaaaccacat ccctttcact
    50341 ttgatcggac agccttatca tcatgagaac gaaatcgttt atgtagataa tgataaccag
    50401 cttctaggta aacaagcaac agaatttttg atccagaatg gacataagaa tatcctgttt
    50461 gtgacaaaca cgactcatga aagtttgtat ttcgaacgat atttcggtta tcaaaaagca
    50521 atgatgatgg ctggtcttcc tgcgttatcc agtgtagtac tagagcatcc ggaggattat
    50581 ctgtcctttg agaaaacgtt gaaagaaaac gaagccacag cattggtggt cattgacgat
    50641 atcttcgccc ttcgcacgat gcagttggcg caaatgtatg gctatgtcat tccagagaca
    50701 ctctccgtca ttagcttcaa caactcgatc ttctcaacat taacacaccc ttaccttaca
    50761 agtatcgata tcgatatctc cgaattagga cgtgtcgcta tgcaaaagct gcgcgaattg
    50821 atccatgaag aattgagcag tggcgtacgt ttggtcatcc ctcatcgctt gatcaaaaga
    50881 gagacagtcg ttcctttaga aaacccatac taaatagctg actcactaaa tacgctgacc
    50941 atttatccta tggtcagctt tttatgtaaa agaagatact acttctaaca cattgtttat
    51001 ctgtaaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    51061 ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac aaaaatttaa
    51121 aaagcaattt tcttgagttt cttcttattt cttggagtta aacgcttctg tcccagcctc
    51181 ttttttcctt ccttcaattt tttacacgtc gacgattcaa taaaaaaccg cctaatattg
    51241 taaagacagg tgcaagaatc gagaaaaagg cgaatggcaa gtaatgcaac gcgctaactt
    51301 tcatcgttcc cataatgaat gtgccgctga ctccccacgg tacgagagaa ttgacggtcg
    51361 cacctgaatc cgctagtgtt cgtgtcaaat aggtttttga taagccaacg tcatcaaaag
    51421 aacgcttgaa tgtttctcct ggaagaatga tggacagata ctgttctcca acgatcagat
    51481 tgattccgat acagctcaag gcagtaaacc cgactaaacg agaaggtcgg tccatctttt
    51541 cttttaattc ttgaacgatg gtatcaacga tcagatattt gatcagtaac ccacctaatg
    51601 ccaatgcaag taaaatcaaa ctagcagaac ctaacatact catgatcccg cctcgagaaa
    51661 gcagttcatc tagctttgga tcaggtgtat ttgcgacata accattcatc aagatgtctg
    51721 ctgcttttcc gaccgtcaaa cttttctcat ggataaaaga aagaacgaga gcagctgtcg
    51781 atcccgctag taatgtaggg atagccggaa tttttttgat tgccccccca aataaaataa
    51841 ctagtggaat caatgtccaa ggagagatcc aaaagctcgc tttcatgttt tggatcattt
    51901 cattgatact agctagatcg gctgaatgac tgttatgtcc taatatcgta taaaaaatca
    51961 acgaaaggac gaatgctggg aaatccgtat aacgcatacc agcgatatgt tcgaatagat
    52021 taactcctcc aatacccgct gccaagttgg tcgtatctga taaaggcgag cagttattcc
    52081 ctaaaaaggc accggaaacg actgcgcctg tagtcattgc atcattgaat ccaagtaaat
    52141 gaccgatccc caaaaaagca attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag
    52201 tcactccgac aattgcacaa acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa
    52261 gtttaaatcc ataaaccata atcgtaggga tggttccaga taaaatccat gaagcaacta
    52321 agacaccaat caaaagaaaa atcaggattg gaatgatccc tggagtgatt cctttgatga
    52381 tcccgtcatg gatctcatcc catgtgaact ttttaaatct gccataaaac aacaaacaca
    52441 tgaaagctgc aaggatcggg atatgtggcg agagctggaa tccgatgatc agtactccta
    52501 ggagtgccaa caacacgatc aatacagcga tactttcttt taaagaaata tttttattca
    52561 tcgtcgatct cctatttcaa atgaaaaggc cctgccatga aaaaactatt cacttggcag
    52621 gacctcctac taaatcagcg acaaaacgtc ggctgaaaga cttatttttt tatttttctt
    52681 ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg atgatcaatg
    52741 gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc aatgtcgctg
    52801 tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag ctgactaccc
    52861 aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct ggatgtttct
    52921 tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa ataacagttg
    52981 tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca taaagcaaag
    53041 caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg ccgtatttgt
    53101 tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact tggatgattg
    53161 tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata aataccatga
    53221 agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca atcaagatag
    53281 gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct acaagcatcg
    53341 cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct tttgtataat
    53401 tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc ccaacaaaaa
    53461 taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca gggaaagctt
    53521 ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg aaggctgcca
    53581 aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat acaccgatgt
    53641 tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc cagtagataa
    53701 ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta ccaaatgtgt
    53761 ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac atttgcaccc
    53821 ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt acccataact
    53881 gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt gatatcggac
    53941 cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta
    54001 acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata aaggtcccaa
    54061 gacccatttt acttacttca gatttactca ccgttgacac tctccttgat tttttggata
    54121 aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc gaatatttga
    54181 ggggattctt tgagtttatt gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg
    54241 atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag aaacagttag
    54301 tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg ccaataattg
    54361 gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt cagcatattc
    54421 ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca atacagttac
    54481 ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa attgtaatgg
    54541 gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact cattaccgta
    54601 aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt tattcattgc
    54661 aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga aatctggata
    54721 agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt cattcaagaa
    54781 gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat atcctgtgac
    54841 attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc ctgcttttga
    54901 accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt caaatacata
    54961 tgtcgcaaag taagagataa cgtctctcat gcggatatct ttgatgacga taccaccggc
    55021 agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg cttcttcgat
    55081 tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg taaagacatt
    55141 gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa
    55201 gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga agtaaatacc
    55261 gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga ttgctccttc
    55321 ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag ctgctagacc
    55381 acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg
    55441 aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg aatagtgttt
    55501 agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac gtgctgagtg
    55561 agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga tttcttctgt
    55621 tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg tcacttcttt
    55681 cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta agtttgctaa
    55741 agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt ttgcaaattc
    55801 cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg caacgttatt
    55861 accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg tttcagagtt
    55921 catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac gcatccgcgc
    55981 tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac tagcactgct
    56041 tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac gccagcctaa
    56101 atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct cagctttgtc
    56161 cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca tagtgaaacc
    56221 ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct tctattctgt
    56281 aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta tccgaattgt
    56341 tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa
    56401 agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat tggaatgttg
    56461 taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat tactttggcc
    56521 aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa ttcatcggat
    56581 ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat agctccgttt
    56641 gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac aagcagttcc
    56701 acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa tccttgcgcg
    56761 atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt tattttttgc
    56821 gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc caaacggcgt
    56881 tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc gatttcttct
    56941 tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc ttgattcaac
    57001 cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac ggcaccgcct
    57061 gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt taaaccgaac
    57121 gcttctgctt ctggtccttc taattttcgg attaggtcta atccagctgt gatatttccc
    57181 cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag
    57241 tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg agattcctac ttccaaacgg
    57301 ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc gtagtctctc
    57361 aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt
    57421 gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt atgttgcact
    57481 tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct
    57541 gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct tttcatcatc
    57601 ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac gccgcaataa
    57661 agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta
    57721 atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc cgtgtagttt
    57781 ttttatgaaa gcaggaacct gctagcatat gtaattaatt atttcttcag gataggatag
    57841 gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg taccacccaa
    57901 tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc gtggcaatca
    57961 ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg cagcgaccac
    58021 cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc ttgttattat
    58081 taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag gatatttttt
    58141 ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt tattttcaat
    58201 tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag aagtcacttt
    58261 actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa
    58321 agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc acttctgctt
    58381 taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag
    58441 aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca gatgataagc
    58501 aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac caagcgattg
    58561 cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt cttcaaacac
    58621 taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc tctcatgtct
    58681 ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag gcacaaggat
    58741 acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa
    58801 atgattttgt tttagttaat ttgaatgatt tgatccaaaa agacgaacaa ggaaatcttt
    58861 cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca caagatgatg
    58921 tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc gtatcaaata
    58981 acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt gactttgaca
    59041 tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat cgaggcgcaa
    59101 aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca tcaaaatctc
    59161 aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa gtggcggaac
    59221 agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg aatatgactc
    59281 aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta
    59341 tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc gactggcagc
    59401 cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc gatattttct
    59461 gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc tatcgaaatg
    59521 ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa aatcctattc
    59581 ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa tctgcttgat
    59641 tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt ttcggctttt
    59701 ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc cattctgctg
    59761 tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc attgcttgaa
    59821 aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct ttccgccctc
    59881 gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc atcgcgtcag
    59941 aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa aattgtttgt
    60001 tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt tcttcaaatg
    60061 gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata aatgcaaaaa
    60121 atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc gctttactta
    60181 cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg tctgtgttca
    60241 tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc ttttcaaaac
    60301 cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag ttcaaattta
    60361 ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt ccttgtttat
    60421 gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata aaaaaagttt
    60481 tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta tatattttta
    60541 gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg aaaaagagct
    60601 ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt gaagtcgttg
    60661 gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt ttagggatta
    60721 taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa gatagtttat
    60781 ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga aatttgactg
    60841 gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag
    60901 atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag
    60961 gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat ctctatttat
    61021 ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa gaagaagttg
    61081 aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc agtgaagtcg
    61141 agcgactagc ggataaagtc gtgatcatac gcgaaggaac actcgatgaa gaaggaacac
    61201 tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa gcagagaata
    61261 gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat
    61321 ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga
    61381 aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat tatgaaggat
    61441 gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt tctcaaaacc
    61501 ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct ttttctggtg
    61561 gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga ttatacgaaa
    61621 ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg aatgctgctg
    61681 agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg
    61741 ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga
    61801 ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg
    61861 aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg atttttttcc
    61921 aagcggattc gatcactgca gaaggtgctt ggctttttgg gctatctgta ggtagtatgg
    61981 ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt tcagccagtt
    62041 cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca ataacagatg
    62101 tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg acctatccat
    62161 ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta atcttgataa
    62221 tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc cccgaacgtg
    62281 aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta ttctatctaa
    62341 ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg gcatacggct
    62401 ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa cagatgttta
    62461 tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggta gtcttgattt
    62521 gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg
    62581 gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata
    62641 tgttactagc tattctattt gctatattgg caatcctgat tagttctggt gcactagggc
    62701 tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag
    62761 ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg
    62821 gatggcttcc tcgaatcggt aaacttgctt atatctactt agcttactcc tttgcactca
    62881 attactttgg cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct
    62941 gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta
    63001 tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag
    63061 gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac
    63121 atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac
    63181 ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa
    63241 agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa
    63301 gtgatccttc cctgtcgctt tagctaagtc aagcagatag agagcgactc gttcttctac
    63361 tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat
    63421 tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg
    63481 tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac
    63541 gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc
    63601 ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga tcactaattg
    63661 aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag
    63721 tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac
    63781 acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa
    63841 acataacttc ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga
    63901 accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt
    63961 gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa
    64021 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
    64081 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
    64141 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
    64201 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
    64261 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
    64321 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
    64381 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
    64441 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
    64501 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
    64561 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
    64621 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
    64681 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
    64741 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
    64801 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
    64861 aaactgcgat gagcatcacc gcagttttat ggctctataa tttatgggac tgatgaatca
    64921 gaattgattc ttcagcccca tttttatttt aggtattaaa aaacatatcg ttctctagta
    64981 tgattaaatc acgacaaaat aaacaactga gaggacgata tgctatatga atgattctat
    65041 caaaaaaatg ctgcgattaa tagagaaaga tttgatgatt acagaggtct cttacgagac
    65101 ctttcagaag aaaaagacgt tgatcgtcga tgctgttttc tcacctgctc cccatac
//
LOCUS       DAZAJZ010000006        57239 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000006 DAZAJZ010000000
VERSION     DAZAJZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.006"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..411
                     /locus_tag="ACG86D_000394"
     CDS             181..411
                     /locus_tag="ACG86D_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN3139574.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            508..1809
                     /gene="murA"
                     /locus_tag="ACG86D_000395"
     CDS             508..1809
                     /gene="murA"
                     /locus_tag="ACG86D_000395"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN3139575.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1822..1998
                     /locus_tag="ACG86D_000396"
     CDS             1822..1998
                     /locus_tag="ACG86D_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN3139576.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2133..2429
                     /gene="yidD"
                     /locus_tag="ACG86D_000397"
     CDS             2133..2429
                     /gene="yidD"
                     /locus_tag="ACG86D_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN3139577.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2614..3081
                     /locus_tag="ACG86D_000398"
     CDS             2614..3081
                     /locus_tag="ACG86D_000398"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139578.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3166..4050
                     /locus_tag="ACG86D_000399"
     CDS             3166..4050
                     /locus_tag="ACG86D_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3139579.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4164..4400
                     /locus_tag="ACG86D_000400"
     CDS             4164..4400
                     /locus_tag="ACG86D_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN3139580.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4488..4568
                     /locus_tag="ACG86D_000401"
     tRNA            4488..4568
                     /locus_tag="ACG86D_000401"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4522..4524,aa:Tyr,seq:gta)
     gene            4575..4646
                     /locus_tag="ACG86D_000402"
     tRNA            4575..4646
                     /locus_tag="ACG86D_000402"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4607..4609,aa:Gln,seq:ttg)
     gene            4771..4941
                     /locus_tag="ACG86D_000403"
     CDS             4771..4941
                     /locus_tag="ACG86D_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139581.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5161..5868
                     /locus_tag="ACG86D_000404"
     CDS             5161..5868
                     /locus_tag="ACG86D_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3139582.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5927..6571)
                     /locus_tag="ACG86D_000405"
     CDS             complement(5927..6571)
                     /locus_tag="ACG86D_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN3139583.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6697..6984
                     /locus_tag="ACG86D_000406"
     CDS             6697..6984
                     /locus_tag="ACG86D_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139584.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7092..7412
                     /locus_tag="ACG86D_000407"
     CDS             7092..7412
                     /locus_tag="ACG86D_000407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3139585.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7493..8806
                     /locus_tag="ACG86D_000408"
     CDS             7493..8806
                     /locus_tag="ACG86D_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN3139586.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8803..9522
                     /locus_tag="ACG86D_000409"
     CDS             8803..9522
                     /locus_tag="ACG86D_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN3139587.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9650..10201
                     /gene="raiA"
                     /locus_tag="ACG86D_000410"
     CDS             9650..10201
                     /gene="raiA"
                     /locus_tag="ACG86D_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN3139588.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10423..12957
                     /gene="secA"
                     /locus_tag="ACG86D_000411"
     CDS             10423..12957
                     /gene="secA"
                     /locus_tag="ACG86D_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN3139589.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13083..14184
                     /gene="prfB"
                     /locus_tag="ACG86D_000412"
     CDS             join(13083..13154,13156..14184)
                     /gene="prfB"
                     /locus_tag="ACG86D_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN3139590.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14289..14975
                     /gene="ftsE"
                     /locus_tag="ACG86D_000413"
     CDS             14289..14975
                     /gene="ftsE"
                     /locus_tag="ACG86D_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN3139591.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14968..15852
                     /gene="ftsX"
                     /locus_tag="ACG86D_000414"
     CDS             14968..15852
                     /gene="ftsX"
                     /locus_tag="ACG86D_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN3139592.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16004..17452
                     /gene="cls"
                     /locus_tag="ACG86D_000415"
     CDS             16004..17452
                     /gene="cls"
                     /locus_tag="ACG86D_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN3139593.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17789..18646
                     /locus_tag="ACG86D_000416"
     CDS             17789..18646
                     /locus_tag="ACG86D_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN3139594.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18761..19681
                     /gene="pstC"
                     /locus_tag="ACG86D_000417"
     CDS             18761..19681
                     /gene="pstC"
                     /locus_tag="ACG86D_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN3139595.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19681..20565
                     /gene="pstA"
                     /locus_tag="ACG86D_000418"
     CDS             19681..20565
                     /gene="pstA"
                     /locus_tag="ACG86D_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN3139596.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20580..21386
                     /gene="pstB"
                     /locus_tag="ACG86D_000419"
     CDS             20580..21386
                     /gene="pstB"
                     /locus_tag="ACG86D_000419"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN3139597.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21402..22160
                     /gene="pstB"
                     /locus_tag="ACG86D_000420"
     CDS             21402..22160
                     /gene="pstB"
                     /locus_tag="ACG86D_000420"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN3139598.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22173..22850
                     /gene="phoU"
                     /locus_tag="ACG86D_000421"
     CDS             22173..22850
                     /gene="phoU"
                     /locus_tag="ACG86D_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN3139599.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23427..24119
                     /locus_tag="ACG86D_000422"
     CDS             23427..24119
                     /locus_tag="ACG86D_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN3139600.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24116..24883
                     /locus_tag="ACG86D_000423"
     CDS             24116..24883
                     /locus_tag="ACG86D_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN3139601.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24983..26551
                     /gene="liaX"
                     /locus_tag="ACG86D_000424"
     CDS             24983..26551
                     /gene="liaX"
                     /locus_tag="ACG86D_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN3139602.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26848..27168
                     /locus_tag="ACG86D_000425"
     CDS             26848..27168
                     /locus_tag="ACG86D_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN3139603.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27168..27518
                     /locus_tag="ACG86D_000426"
     CDS             27168..27518
                     /locus_tag="ACG86D_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN3139604.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27685..28623
                     /gene="hprK"
                     /locus_tag="ACG86D_000427"
     CDS             27685..28623
                     /gene="hprK"
                     /locus_tag="ACG86D_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN3139605.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28638..29468
                     /gene="lgt"
                     /locus_tag="ACG86D_000428"
     CDS             28638..29468
                     /gene="lgt"
                     /locus_tag="ACG86D_000428"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN3139606.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29512..30537
                     /locus_tag="ACG86D_000429"
     CDS             29512..30537
                     /locus_tag="ACG86D_000429"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN3139607.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30552..31490
                     /gene="galU"
                     /locus_tag="ACG86D_000430"
     CDS             30552..31490
                     /gene="galU"
                     /locus_tag="ACG86D_000430"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN3139608.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31615..32055
                     /locus_tag="ACG86D_000431"
     CDS             31615..32055
                     /locus_tag="ACG86D_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN3139609.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32058..32660
                     /locus_tag="ACG86D_000432"
     CDS             32058..32660
                     /locus_tag="ACG86D_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN3139610.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32719..33825)
                     /locus_tag="ACG86D_000433"
     CDS             complement(32719..33825)
                     /locus_tag="ACG86D_000433"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN3139611.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34035..35036
                     /gene="ccpA"
                     /locus_tag="ACG86D_000434"
     CDS             34035..35036
                     /gene="ccpA"
                     /locus_tag="ACG86D_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN3139612.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35373..37718)
                     /locus_tag="ACG86D_000435"
     CDS             complement(35373..37718)
                     /locus_tag="ACG86D_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN3139613.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37997..38899
                     /locus_tag="ACG86D_000436"
     CDS             37997..38899
                     /locus_tag="ACG86D_000436"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN3139614.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39112..40887
                     /locus_tag="ACG86D_000437"
     CDS             39112..40887
                     /locus_tag="ACG86D_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN3139615.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40953..42539)
                     /locus_tag="ACG86D_000438"
     CDS             complement(40953..42539)
                     /locus_tag="ACG86D_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN3139616.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42790..43014
                     /locus_tag="ACG86D_000439"
     CDS             42790..43014
                     /locus_tag="ACG86D_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN3139617.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43155..44906
                     /locus_tag="ACG86D_000440"
     CDS             43155..44906
                     /locus_tag="ACG86D_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN3139618.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44906..46690
                     /locus_tag="ACG86D_000441"
     CDS             44906..46690
                     /locus_tag="ACG86D_000441"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139619.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47016..47867
                     /gene="licT"
                     /locus_tag="ACG86D_000442"
     CDS             47016..47867
                     /gene="licT"
                     /locus_tag="ACG86D_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN3139620.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47963..49843
                     /locus_tag="ACG86D_000443"
     CDS             47963..49843
                     /locus_tag="ACG86D_000443"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3139621.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49883..50785)
                     /locus_tag="ACG86D_000444"
     CDS             complement(49883..50785)
                     /locus_tag="ACG86D_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3139622.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51016..52419
                     /locus_tag="ACG86D_000445"
     CDS             51016..52419
                     /locus_tag="ACG86D_000445"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN3139623.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52419..53852
                     /locus_tag="ACG86D_000446"
     CDS             52419..53852
                     /locus_tag="ACG86D_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN3139624.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            53997..54923
                     /gene="trxB"
                     /locus_tag="ACG86D_000447"
     CDS             53997..54923
                     /gene="trxB"
                     /locus_tag="ACG86D_000447"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN3139625.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55137..56864
                     /locus_tag="ACG86D_000448"
     CDS             55137..56864
                     /locus_tag="ACG86D_000448"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN3139626.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56879..57229
                     /locus_tag="ACG86D_000449"
     CDS             56879..57229
                     /locus_tag="ACG86D_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN3139627.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        18566 a         9957 c        12350 g        16366 t
ORIGIN      
        1 taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt tagcatttat
       61 ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt ccttttatac
      121 attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa ggacgtcatt
      181 atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc gtttatctac
      241 ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc aatgcataat
      301 acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac tgcaagtaat
      361 tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta aattttaaaa
      421 aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt aagtgtatgc
      481 tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg tggcaatcaa
      541 ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat tttagctgct
      601 agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct ttctgatgtc
      661 ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga tgaacaaaag
      721 aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt
      781 agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa tggacatgca
      841 aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa
      901 ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga agcaattgcg
      961 gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc tactcaaaat
     1021 atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa
     1081 ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt ctatggagct
     1141 ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc
     1201 gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac acaaggcaat
     1261 gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa attgatcgaa
     1321 atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc aaaacatatc
     1381 ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat gcaagctcaa
     1441 atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa
     1501 aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga
     1561 aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac
     1621 ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat cacacgtgtg
     1681 cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact acagcaatta
     1741 ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac agcacaaacg
     1801 attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac tttgataaag
     1861 attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat gatcggatat
     1921 ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg gattcatatt
     1981 cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat gaagtgagcc
     2041 atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc gcgatccctt
     2101 tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt tatttagtta
     2161 gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat tacccaacct
     2221 gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg acggtaatgg
     2281 ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat
     2341 tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat aaaaaggttg
     2401 tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc ttcttatatt
     2461 ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa aaaaaaggtt
     2521 gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt gtcaaagtaa
     2581 caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa caagaaagat
     2641 ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca agtatatgtt
     2701 tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt actgatctat
     2761 gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac gtatcaagga
     2821 aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca actcgcttcc
     2881 agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt acatctctat
     2941 caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca tcattactct
     3001 gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc aattcactta
     3061 cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca aatgctaaat
     3121 agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg aatattagtt
     3181 tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga gattaaaggt
     3241 aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt
     3301 tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc tcctggagta
     3361 gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat tcatggattt
     3421 aatattttcc gagatttgga aactctgttt caacttccag taacgattga aaatgacgcc
     3481 aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca agatgttgct
     3541 tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca aatcaataaa
     3601 ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga taagacattc
     3661 agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa aggtctcgat
     3721 aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga tgcaatagca
     3781 aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag tattcaattt
     3841 tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga agggctcatt
     3901 gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca tgatttcgaa
     3961 ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc agcagcaaac
     4021 tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag cttgcataaa
     4081 agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt gctgcagtat
     4141 gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt gaagtgcgta
     4201 tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag ttatttatag
     4261 ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa attttctttg
     4321 gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac
     4381 actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat
     4441 ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga ggagtagcga
     4501 agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt cgaatccact
     4561 ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga ctccctcatg
     4621 cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt tagttcgaga
     4681 gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta agatagagtt
     4741 aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt agacgaagca
     4801 gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt gaatattccg
     4861 aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa
     4921 tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt ttcatcgatt
     4981 ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc tgacaatagg
     5041 agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct ttatcttttt
     5101 cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg ggtgtacgtt
     5161 atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa tgaaatcgaa
     5221 caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc agtgaaattc
     5281 aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga aggaatctta
     5341 gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga aaaaatgtct
     5401 ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc tagtcggaca
     5461 atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa
     5521 gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt
     5581 gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga gattactgaa
     5641 tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg
     5701 attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa acgaggagat
     5761 gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt tgagtatgtg
     5821 cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa aacaggtgat
     5881 ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat atatgatcag
     5941 ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt ccgttcagca
     6001 gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca cagatgacgc
     6061 taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca agataattct
     6121 gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc aaggtacctt
     6181 ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct cgtattaatc
     6241 cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct acattgatca
     6301 gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt ggttctccat
     6361 catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg cagttatggt
     6421 tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg tcctctgatt
     6481 ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca ctttgtccat
     6541 tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca ttttctttat
     6601 tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa ttgctgtagt
     6661 atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat gaaaaagtac
     6721 gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt gacctttacc
     6781 caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag ggaaagtaca
     6841 gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg gataaatgca
     6901 ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa taaattagtg
     6961 aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac aaatttcttt
     7021 ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag
     7081 gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt atgagcacaa
     7141 gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac tctgatattt
     7201 tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg gatgtgttgt
     7261 tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg gaaccaaaag
     7321 gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc gaaaaagtgt
     7381 tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat cgaacaagcg
     7441 atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata atatgaatca
     7501 tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg aggaggtaaa
     7561 tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga ttctttgtca
     7621 tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg gttatttttg
     7681 tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt tgtttgatca
     7741 gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta ctgaaaaaca
     7801 aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc ttatttgggc
     7861 tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa ctttaaaagc
     7921 tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg
     7981 ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata cagcaacggc
     8041 ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt tttatcggtc
     8101 ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg atgcaggatt
     8161 agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact taagtgccac
     8221 acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca aacttgcttt
     8281 acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata attggcgaca
     8341 aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc aattaattaa
     8401 agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt tgaaagccac
     8461 acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag acaatcagcg
     8521 ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa ccacgatgat
     8581 ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag cggatcatcc
     8641 ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta aagggcctta
     8701 tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc gaaaagcttg
     8761 tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg cagttgctgc
     8821 agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc gaaatacaga
     8881 ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac aagaggctgt
     8941 tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa agtaaattct
     9001 atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac ctatgatttt
     9061 cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa tcaatacaag
     9121 tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca gataaaaaaa
     9181 gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg aacagaaaga
     9241 ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca gatgctactc
     9301 aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag attggagatg
     9361 cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa aatattgatt
     9421 attgatgatg tctatacaac aggacagact cttttccacg cagcagattg tttgcagatc
     9481 gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg tggaaatttg
     9541 atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag aagagggaaa
     9601 gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta tgtttagata
     9661 taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa
     9721 agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc atgtgaactt
     9781 aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac cttatcttgt
     9841 attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg ttgttgacaa
     9901 attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg
     9961 catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg aaaatgattc
    10021 tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc
    10081 agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg cagaaacaaa
    10141 tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg aaacagactg
    10201 atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct gccgcaagtt
    10261 tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt cttactttaa
    10321 tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta aagtctacat
    10381 agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa tttgattcgt
    10441 tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc aaaaaaagtt
    10501 gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc aaaaactgct
    10561 gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc ggaagctttt
    10621 gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca cgtacaattg
    10681 atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg tgaaggtaaa
    10741 accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg ggtacacgta
    10801 gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga actttataat
    10861 ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga aaaacgcgag
    10921 gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga ctacttgaga
    10981 gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta tgccattgtc
    11041 gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa
    11101 gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg tttgaaagaa
    11161 gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga agcagggatc
    11221 gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa tactgcattg
    11281 acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga tatcgattat
    11341 gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat catggatgga
    11401 cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt agagatcgaa
    11461 gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat gtacaagaaa
    11521 cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga aatttataac
    11581 atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg tccagatttg
    11641 ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa agatcgctac
    11701 cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga gttattatca
    11761 gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa tcactttaaa
    11821 gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc taccaacatg
    11881 gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg tggtctagca
    11941 gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg aggacgtgca
    12001 ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga tgatttgatg
    12061 aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat cggtgaggaa
    12121 gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca aaaacgtgta
    12181 gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt gatgcgtgaa
    12241 caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa aagcttatcc
    12301 aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc gcatacacaa
    12361 ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag tactcttgtc
    12421 catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga gatcaaagag
    12481 tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact aaatggtcca
    12541 gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc taaatggaca
    12601 gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc ttatggacag
    12661 aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga tatggtaggc
    12721 gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca aaacgtacag
    12781 cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc caaagaaaat
    12841 agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag aaatgatcct
    12901 tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc aaactaattt
    12961 ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac cttctttctg
    13021 ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa gaaggaaaaa
    13081 acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag gtcacaagct
    13141 tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc agaagcaaaa
    13201 atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat cagtgaaaac
    13261 aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact ggaagaaatc
    13321 gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga agaagcagaa
    13381 acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat gttgttagat
    13441 ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg cggaacggag
    13501 tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga gcagcatggg
    13561 tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa gagtgtgacc
    13621 ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg tgttcatcgc
    13681 cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt ttgttcggta
    13741 gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga tgatttgaaa
    13801 atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac agaatctgct
    13861 gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca acgttcacaa
    13921 ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca attagaagtg
    13981 gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat cggctggggt
    14041 tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca tcggacaaac
    14101 gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt tattgacggc
    14161 tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa ttgtaagatt
    14221 ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa agatatggag
    14281 agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt acgaccgcta
    14341 tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt ggtccgtctg
    14401 gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa
    14461 ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt ccttatctac
    14521 gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa acagtatatg
    14581 aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc aaaaaacgag
    14641 taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt cctagcgaat
    14701 tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat acgcctaaag
    14761 tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg gaaatcatga
    14821 aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca cataacagta
    14881 cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc attattcgag
    14941 atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca tttattagaa
    15001 agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag tgcggtaacg
    15061 atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac taaattagct
    15121 caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac aaaagacgaa
    15181 gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa agttgaattt
    15241 tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc ctggaaattg
    15301 tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac tgatccttct
    15361 tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg ttctgattac
    15421 ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac atggggatta
    15481 gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac gattcggatc
    15541 acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc aaaaaatgga
    15601 tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg tgcgattctt
    15661 ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa tccatcatta
    15721 ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat caatctttta
    15781 atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat gagacgtttc
    15841 ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt cgttccagct
    15901 ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt actttaaaaa
    15961 acaacttggg tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa ttatcgttga
    16021 caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca tcgtttttcg
    16081 agaaagaaag gaaactgctc aaacatgggc atgggtgctc gttttgatgt tcattccagt
    16141 tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga aaatctttga
    16201 tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca aacaggcatt
    16261 gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac aattgatcta
    16321 tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca ctttatttac
    16381 agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaggcag aagaccatat
    16441 ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc gagatgcatt
    16501 gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat ggggttccac
    16561 acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaag tcgcattttt
    16621 ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc atcgtaaaat
    16681 cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa atgaatatct
    16741 aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt acggagaagc
    16801 ggtgtatacc ttgcaaaacc gttttttgat ggactggaat tctcagcata caaaagaagt
    16861 aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag ctgttcaaat
    16921 cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt tgaaaatgat
    16981 ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg atggatcgat
    17041 tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc aaatacctaa
    17101 caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag agctaatcga
    17161 gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga cgatgattat
    17221 cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat ttcgcttgga
    17281 ctttgaggtc aatacactgg tttatgatga acgaatggct tcacgtgtaa gaaaagcttt
    17341 ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa accgaggaat
    17401 aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt aaaacataaa
    17461 aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg gggccatttc
    17521 tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact ttggggtatc
    17581 tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag agacacattt
    17641 caaagccagt atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt
    17701 tattttgtta tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa
    17761 catactttag gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat
    17821 aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac
    17881 ggctgttggc tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac
    17941 agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca
    18001 agtacagtca ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg
    18061 gatcgatgcc tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt
    18121 gaacaaggat attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg
    18181 cgagatcaaa aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg
    18241 cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc
    18301 tgttcaggca caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc
    18361 cggtgcaatc agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt
    18421 taacggtgta gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata
    18481 tgagcatatg tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat
    18541 cttatctgat gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca
    18601 tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat
    18661 ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca
    18721 ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt
    18781 attattaaca aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt
    18841 atgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg
    18901 attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg
    18961 gaatccaagc caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac
    19021 aggttcgttt attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc
    19081 agcagtattc atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat
    19141 cgagctgctt gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt
    19201 gccttttgtc agaagtattt ttggcgggac tgggtttggt atcttagctg gtacatttgt
    19261 gttgttcgtt atgatcttgc cgactatcac aacgatgact gttgatgcat taaaggcagt
    19321 gccacggcac tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta
    19381 caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc
    19441 tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc
    19501 aacaggtctt actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa
    19561 tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt
    19621 gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata
    19681 atgaacgcaa aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc
    19741 atcgtattga ttttagcagc tttactactt tatatcttag ttcggggaat accgcacatt
    19801 tcatggtcct tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc
    19861 cagctattta actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct
    19921 ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt
    19981 cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc
    20041 ttggttttcg ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg
    20101 actgtattca acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat
    20161 tacacacaac gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa
    20221 gtggtggttc cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga
    20281 atttttggtg aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat
    20341 tttacgaact ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa
    20401 acattagctg tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct
    20461 gtttcggctg gcgcttctgc cgtgttgatt attgctgttt tactgttcaa cttcggcgct
    20521 cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa
    20581 tgaaaaaata taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag
    20641 cactatatac ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg
    20701 atctggaatt tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat
    20761 ctacgtatct tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg
    20821 ggaaaatcat gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc
    20881 gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca
    20941 atattacatt tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg
    21001 ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa
    21061 gtgccttggc tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga
    21121 tgaaacctga tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag
    21181 gaacggttga agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc
    21241 ataacatgca acaagcagct cgtatcagcg actatactgc ctttttctat ttaggaaaag
    21301 taatggaata tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag
    21361 attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gagattattt
    21421 catctaaaga tcttcatctg tattatggaa agaaagaagc actaaaaggg atcgatctaa
    21481 gcttcaatca aggggaaatc actgcgatga tcggtccttc tggttgcgga aaatccactt
    21541 atctgcgttc tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg
    21601 ttttatataa agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag
    21661 aaatcggcat ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca
    21721 tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag
    21781 aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt
    21841 tatctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc
    21901 cggaaatcat cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa
    21961 tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata
    22021 tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg
    22081 aatacaatga tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata
    22141 tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat
    22201 tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca
    22261 agtcagttcg tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg
    22321 atataaatat caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat
    22381 tgcaacagcc agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg
    22441 atttagaacg tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag
    22501 ggcagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa
    22561 aaatggtgga caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga
    22621 tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca
    22681 aaaatatgca ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca
    22741 gttatctaga acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg
    22801 ctacagggaa aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg
    22861 tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt
    22921 ctgcatcaag taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct
    22981 ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac
    23041 aaaacatgtc acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa
    23101 ttagcatcat cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag
    23161 ccgctttata tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa gaggatcatc
    23221 aattgattac aggaaaactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg
    23281 ttccttatca aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag
    23341 tccaaatgca tggagatgaa tggcagctag tagcaaaaaa aaatgatggc tggcttttct
    23401 tcttcacaag tatcttcatt ggcgccatgt tgcttattgg tggaaaacag ggtctatttg
    23461 ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta
    23521 aaacagaagg gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta
    23581 ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag
    23641 ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc
    23701 aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt
    23761 ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga
    23821 cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag
    23881 gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata
    23941 tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa
    24001 cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag
    24061 tattatcgat ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa
    24121 tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca
    24181 aggtatgtat attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt
    24241 cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc
    24301 tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag
    24361 tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt ttttagcaat
    24421 ccaaggtttt acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat
    24481 tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga
    24541 tgcgagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc
    24601 tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt
    24661 aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga
    24721 tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat
    24781 ggctattctt accacattgt ccgcactact tgttcttcct ttaacctgcg tgactacagc
    24841 atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc
    24901 ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat
    24961 tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa
    25021 aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga
    25081 cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca
    25141 ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga
    25201 tccaaaggaa agagaaagac aagatcaaga aaacttagaa aagatcttag atgaactagc
    25261 aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa
    25321 taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt
    25381 aggcgagttg acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga
    25441 attagaagct agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa
    25501 agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt
    25561 gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc
    25621 agaagctgga tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac
    25681 agtgaatgac aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac
    25741 gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc
    25801 agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa
    25861 gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca
    25921 gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga
    25981 ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg
    26041 caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa
    26101 catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat
    26161 cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac
    26221 atttggtgcg acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac
    26281 gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt
    26341 cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa
    26401 cagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt
    26461 gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa cgacaacagg
    26521 aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg
    26581 agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg
    26641 ccgatagtct atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc
    26701 gagtcacaag gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg
    26761 accgagaatt ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca
    26821 tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atctaataaa
    26881 aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact
    26941 gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc aattatttta
    27001 tacattttat tagctttgat tatcccattt gcgcgttctg cacgaacaaa ctatggacat
    27061 aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa
    27121 cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc
    27181 aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg
    27241 tccatgtgag cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata
    27301 tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc acattgggat
    27361 tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag
    27421 aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg
    27481 tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc
    27541 ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa
    27601 aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac
    27661 tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa
    27721 aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact
    27781 ggggacatct cacgcccagg actagagttg actggttatt ttaattatta ttcccataat
    27841 cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag
    27901 cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga
    27961 ggattaccag ctccagaaga aatgatccag gcagcaaata aaaatgggat agctgtactg
    28021 cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc
    28081 cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg
    28141 attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggc
    28201 catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc
    28261 ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg gatcattgat
    28321 gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt
    28381 tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc
    28441 gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac
    28501 gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac
    28561 gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac
    28621 tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga
    28681 ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgct atgtggctta
    28741 gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt
    28801 ggggattgcc tatctccatt attggcgctc gcttatatta tgttttattt gatcttcccc
    28861 agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg
    28921 gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt
    28981 tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt
    29041 ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa
    29101 gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac
    29161 caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac
    29221 gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata
    29281 gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga
    29341 tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt
    29401 ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa
    29461 ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat
    29521 agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa
    29581 aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc
    29641 caacacacga atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc
    29701 accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa
    29761 gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc
    29821 catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag
    29881 gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa
    29941 gaagtagcaa aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca
    30001 aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg
    30061 atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt
    30121 cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag
    30181 atcagccgtt taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga
    30241 ataggcgatc tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac
    30301 ttgttaggac aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa
    30361 ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa tgatggaaat cgaaatgccc
    30421 atcactcaaa caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa
    30481 gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct cattaacaat tttttgaagg
    30541 agcgaatgta tatgaaagtt aaaaaagcag ttattccagc agcaggatta ggaacaagat
    30601 ttttaccagc aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa
    30661 tccagttcat cgttgaagaa gcgttgaaat caggtatcga agacatcttg atcgttacag
    30721 gaaaagctaa acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc
    30781 taagtgaaaa aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact
    30841 tgcatttcat ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag
    30901 cctttattgg aaatgaacca tttgtcgtta tgctaggcga tgatataatg gaagacgaag
    30961 taccattgac acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg
    31021 ttatgcaagt tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat
    31081 tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac
    31141 caagtgattt agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt
    31201 ccgagcaaca accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga
    31261 ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca
    31321 aatttggttt tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac
    31381 ctttacgtga gtacatcctg tctttaggta aaacattaga agcagaagga agcaagatcg
    31441 atcataatcc aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc
    31501 taacgagctt cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa
    31561 aaaacacgaa aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca
    31621 ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc
    31681 gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa
    31741 gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa
    31801 ggcttactgg tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc
    31861 gatccattgt tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct
    31921 agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt
    31981 gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg
    32041 acaggaggaa aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag
    32101 gcacagccgc agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg
    32161 atgaattagc tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg
    32221 cgaaagaaaa atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc
    32281 aacaagcagg tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag
    32341 caggaagtga atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta
    32401 atcgtttcaa aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg
    32461 ctaaagatac ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag
    32521 atactgtcaa tgaaggaatg tcagaagcaa aaccagttgc agatgactca aaagaaatga
    32581 tgaaaatggc aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag
    32641 attcatctac aaatcactaa aatcacaaat ttatagaata aaaaggtggg agcggactag
    32701 ctctcacctt ttttactctt atttttctaa aacaagtaat tcttttggtg tagtcgtgaa
    32761 tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc ctactttttc
    32821 aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta ctaagtcatt
    32881 tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac ctaaacggtg
    32941 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
    33001 acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt caagagtgat
    33061 gttgtagatt ttttcttgga agtcggttgg tttttgataa gcaattgtac gagtagcatc
    33121 actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat tcggtgaaat
    33181 cttcgtattt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    33241 gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcaattt ctgcaatgat
    33301 cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg caacatcggc
    33361 ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca gttgtagacg
    33421 ttggatgatg gaagttagat cagtgtaaga atccgttccc gtcaagatat cagagatagc
    33481 atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag gctgaccata
    33541 gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta aatagccacg
    33601 gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag gagtgaaaag
    33661 gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag gatcactttc
    33721 gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt cgacttggtg
    33781 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    33841 ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc aacgttctaa
    33901 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attcttgaga
    33961 ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    34021 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    34081 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtt aaaccagcaa
    34141 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac gcagttgcgc
    34201 gtggcttggc aagtaagaaa acaacaacag ttggtgtaat catccctgat gtcagcaata
    34261 tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac aaatacaata
    34321 ttatcttagc aaattcagat ggcaacgacc aaaaagaagt caatgtattg aataacttac
    34381 tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac gaaatccgtg
    34441 gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat ccagatgaac
    34501 aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa agatgcagta aacaaattgg
    34561 caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat cccatcaatg
    34621 gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta ccatatagcg
    34681 aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta gtcgatcgga
    34741 tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc atcggtattt
    34801 tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc atcacaagta
    34861 acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca caaccattat
    34921 acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    34981 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca aaataataaa
    35041 aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc tgcgttacaa ggagcaattt
    35101 caaaaaacag catctcatgt ttgatgaata ttgtgactcg tgattcggag ttggcaggag
    35161 aacaccgttt atttgggaaa aggggttatg acaaaatttt gttataatcc tttttttgtg
    35221 tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg tatataaaac aatccgtgta
    35281 tagatgctgc attttgttca ccaccatcaa aaaaataagg gactgtgaca atactattgt
    35341 cacaatccct tattactgtg tattctatta aatcaatcat ctttctcatc tgattcttta
    35401 gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt atagttttca
    35461 tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg gtccattttt cgcccatagc
    35521 gattcaactg tttttcctgg tgtttggatg gattttttat tgacagtaac ttttccaggt
    35581 gtttgaccag taaatccaga tacttttttc ttcatcacat cagatgataa ttcgaatttc
    35641 tcatcagtgc caaaaatgtc aggttttgct tggtaagctt gattgatcag attggcaaga
    35701 taattagccg aacgtatcct tgctttgctg tccattccag tattgtcatc gtgccctgcc
    35761 cagctactga tggtaataga cggagtagag acaattagcc atgaatctcg gtattcattg
    35821 gtagaaccag tttttccgac ccaatctgct tttcctagat ttccgtttaa gcttgagatg
    35881 gcatctttga atggtgtagt gatttttgca ttgatcacac tacgcatcat gtcattcatg
    35941 atcgatgccg ttgcttccga atagatccgg acagagttgc tttcgtgttt ataaattaca
    36001 tttcctgcgt tatctgtgat ggaatcgatg atatagcctt cttcatagac gccgccgttt
    36061 gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga catctactgt acctaaagga
    36121 gcagattcaa cgccccaatt gtcatttgcc gggtagttca tctttgccaa gtagtgctcg
    36181 taagcatatt gtttcgatcc accggaattc aacacatctt gatataagtg ataagccggg
    36241 atattgttgg accattctag tgactcacgg acggtttgga aggtgttcga tccttcgtta
    36301 gtagcattga cgattttctc gccagcattt tctccttctt gccaagttgt tgcataatct
    36361 gatacacggg attctgatcc gataagcccc atgtcgattg caggtccata aacaagaact
    36421 ggtttgattg atgagccagc ttgacgttct gtatcgaagg catggttatt ttgattctga
    36481 ctgtaattcc gtccgccaac gaatccgtag atgcggccag ttctgttatc catcaataca
    36541 tttccggttt ctacttgact gccagcacca tcatctaaca tgtatccata attttcaacg
    36601 ccattttgca atgcagcata gacatcttta tcgattgtcg aatggattgt atatccttta
    36661 ttttggatcg tttgctgtgc cttttgataa taagcgtcac tgaccgatga atcagacata
    36721 tcagcgttgt ctttttcagc caattgttgt gcaatgattc ttgttgcttc attcatcaca
    36781 gtataataca agaattctct gtcagtctgt tctgcgattt gttgtggtag aaagtctttt
    36841 gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg taatttggcc ttcacgatac
    36901 atactgaata aaacaaaatc ttttctggca aggccggcag aaagatcttc ttttagcgcg
    36961 cctgtgttcg tgtatggact atacgtgatc gggctttggg gtaatccagc gatataagca
    37021 gcttgcggca gcgtcacatc ttttgcatct acaccaaaga ttccttttgc agcttcctgg
    37081 actccagcaa tgttttgtcc cttattatta cgaccaaatg gtgagacatt caaatacgta
    37141 gcaatgatct catcttttga aaagtatttt tctacttgtg cagaaagaat gatctcgttt
    37201 gctttacgtt tgaatgttgt ttcatctgta aggatctgct gtttgactaa ttgctgtgtc
    37261 aaagttgatc caccagaaga gccaatacca gtagcttcag atactaatgc tcgtaaaaca
    37321 gctttaggaa cgtaaccttg gtgtttatcg aagtattcat cctctgtgct gataattgca
    37381 gttttcaata aaggtgaaat cttatcagat gaaatagtcg tgcgcatcag atctgtctgg
    37441 atttttgata tctctgtatt atctgcataa acaatctttg acgtttctgt aatgtttcct
    37501 agttctgtct gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag ataggcaaaa
    37561 tagccagcgc ctattccaat acctagagct gcgaacatca aaagaagaac agtaataaaa
    37621 acagtcaatg attgaaaaac tctaaaaata atcttaggaa caaaccattt ttcttttttt
    37681 cttctattct gtttttttcg agtatttttg cgtggcaaaa gggtcacctc aactaatggt
    37741 tttatttcgc taattatagc aaataataga acaaaaaaac agacaaggct gtttttggca
    37801 ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga tctttattat aaaagagttc
    37861 ttaagctttt ctttagagaa aatttcagtt actatggaga acagggcaaa gaaaaatcaa
    37921 tcattttctt tgtatatcat cttaccaata tataggaatt gttagtaaca attgcctttc
    37981 aaggaggaat ccatccatgt tattaggctc acacgtcagc atgagcggaa agaaaatgct
    38041 gttaggagca gcagcagaag cagccagtta tggatcttcg acttttatga tctatacagg
    38101 cgcccctcaa aacactagaa gaaaagccat cgaagagatg aatattgatg ccggaaaagc
    38161 gtacatggca gaaaacggtc taacaaatat cgtcgttcat gccccttata tcattaattt
    38221 ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc caatttctga gagaagagat
    38281 tgttcgtgcg gaagctttag gcgctactca aattacactt cacccaggtg ctcatgtagg
    38341 ggcaggaaca gaagccggaa ttgctagaat tattgaaggc ttgaatgaag ttttgacaaa
    38401 agaacagacg gcacaaatcg ctttggagac aatggcaggt aaaggtacgg aaatcggtcg
    38461 aacatttgag gaattagcgc aaatcatcga tggagtaaaa ttaaatgaaa aattatccgt
    38521 tacttttgac acctgtcata cgaatgatgc cggttataat attaaagaag acttttcagg
    38581 tgtaatggaa gagtttgata agatcatcgg gttagatcgg cttaaagtac tccatatcaa
    38641 tgattctaaa aatccgcaag gttcgcataa agaccggcac gccaatattg gttttggaac
    38701 aatcggattc gacacgctga ataagatcgt ccatgatcca atgtttgaca atatttcgaa
    38761 aatcttggaa actccctatg tcggcaaaga taaaaagaaa gcttacgcac catatggaaa
    38821 agaaatcgcc atgttcaaag cacaaatatt cgatccagaa gtgtttcctg agttattagc
    38881 agaagaagtg atactctaag attttctatc tgacagcagc aaaaaaggac caggcacatt
    38941 gccttgtcct tttttgttta agaaacaaag gagtactttt tgcagtcttt ttcatcatgc
    39001 atcttctccc ccctcttttt aaatttagac aaacaaggta tactgttaga aaagtaaaaa
    39061 aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa gatgctgaac
    39121 actttaaaac ccttgatcaa ccaactaaaa aagaaacata catactttat tcttggcata
    39181 gccctattat ttacaattat tcgtttgtat ttatatagta aagcaatcta tggaacagac
    39241 atgtcggcaa cctatgatga ccagctgctg attcgctatg ctcaaaatat tttggacggt
    39301 aaatggctag gggagtatac gactacaaca ctttccaaag gaatctccta cagcttattt
    39361 ttagtacttg cacataaact gcatctttct tatgctgttt tgttcggcat gttcagtatt
    39421 tttgccagta tattggttgc actcgcgttt aaaccgatcg tgaagaacaa gtatcttctt
    39481 ttaagtatct atttttattt tttgttttct ccgattagtt ttacacatga atattccact
    39541 cgaacatatc gtaatgccct cgtgattccg gctgttgaga tcattatcgc ttgtttacta
    39601 gctatttatt accgcaaata cggttctagc agaaaattgc tgccatggtt tttagggctt
    39661 tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt ctatctggct tcttcctttt
    39721 actgtagtag ctttgggtat cagtttcgtc cagatcttgc tagggcaaga cttttctttc
    39781 aaagaagtaa agaagttaaa aaaacgactc tttcttctct cgaaacggga atggctgaaa
    39841 ctttcattgt ttttacttcc tatagttggc ttttccatcg tctatcagtg gatcgaatat
    39901 aaaaatgaga caacatacgg tattgcaacc atcaatgacc gttccagtgg agaatttgga
    39961 cgtttgatga aacaactgat ccgaatagat gatgaaacga acttgaataa agaaaacagt
    40021 gagatatggg tctcacatgc tgcactaaat aaagcattag ccgcatctcc tacattccat
    40081 actgtttcta cccgtattga cgaattatat acgactcacg cgtggactca aggtggaaaa
    40141 gtaaaagaat tacccggaga tatcatcttt tgggcattgc gagatgtggt aagcgaatct
    40201 ggttattacc aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt caaccaagaa
    40261 ttagaagaag cctatcacaa gggaaccatc aaaaagaaaa aagagttcta tctgatggga
    40321 tcaggagatg gaaagacgat gagtgatctg ccaatcgtat tcgatttcct tcaatcagga
    40381 tacgcttatg catggcgata taaagatttc catcaaggag gaaattatag tttcggaacg
    40441 caagaacaag tcaaacaggc tcaagaatta ttaaaccttc cgctaatgga gaattggatc
    40501 gatagtaatg aaccgagaac acctaatttc caattaacaa aatcagcaaa agtcgcgaat
    40561 gtcatcataa aaatttacca agttctggct attcccgtgc tgattgcttc aatagcaggg
    40621 atattactta tgatactagg aaccgtattt gcaaaagctg atcgtgccta ttatagttcc
    40681 tctttgattg tggtcaccgg attaatactg acacaattgg tttttctttt cggtgtctcg
    40741 tggttttgtt cttatgcgcc agaaaaaaag gattattttt taagtgtcta cactggcgca
    40801 ggcgtgccat taacgcaatt catcgtagta acaagcatgc tagttttcag taaattaaat
    40861 ctgaaaaagg ctttatcttc taaataaaaa gacccttggt agctagattt ttaacctaac
    40921 tatcaagggt ctttgtgtgg aaaaatcagt ttctattcga tttcttctac cgtttcctgc
    40981 aaacgctgtt catatttttt gtttccttta tataaatcac caatcgtcca tgccaacagg
    41041 agaaggacaa gaacaatgat tacataagcg atgtgatcag ccaatgctag ttgagcagaa
    41101 gtggcttgtt caccaaagaa actttcgatc tgtcctggta atcctcgtaa gttcaagtaa
    41161 gttagactaa tcacagagaa ccagccaaga agttttacga tgaatgaatt tttaaatcgg
    41221 ctacccatct ccgcttggct gttggtcatc atcagtaaag ggatcatcga aaacggcaac
    41281 gcaaaagcca agaacacctg ggattcattc atcaagttat tcaaagcggt atgttcttcg
    41341 atcgttcctt ttgtacttgt aaacaaaaca caaatcaata caggaatcac agaaagcaat
    41401 cgtgtgacca gtcttcttag ccaaatcggg atacgcatat ggatgaagcc ttccatgatg
    41461 acttgaccgg tcaacgtacc agtaatagta gagttctggc cagaagcaag aagtgctaca
    41521 gcaaataaag tggataagat accagatttt gctacagcaa tcaagatacc attgctcata
    41581 gctgatggat cagataaggc ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg
    41641 gttttgaaga cagcgacacc catgatcaat agcagtgcat tgactagaaa agccatcgac
    41701 aattggatat tagagtccca agtagaaaaa cgtacagctt gtgctacatc ttcttcgtct
    41761 tggtgatcaa tcttccgagt ttgagaaact gctgaatgaa gatataagtt atgcggcatg
    41821 actgttgcac caataatccc taaagctcca gtcagcggag tttcgccgcc gatgaccggt
    41881 gagtctgaga atgtatgact gttagggata aagccagcaa ataaactgtg ccaatctggg
    41941 ttagacaagc caacttgata gacaaaaacg aataaaatga cgaaaattaa gcagacaacc
    42001 agtgcttcga tcttacgaaa cccgactttt gtcagtagta agagaacgaa aacatctaat
    42061 acagtaataa agacggcaat caccaatgga atatcaaaaa gtaagtagag ggcgattgct
    42121 gcaccaatga cttctgcgat gtctgttgcc ataatggcta attcagtcaa gatccataaa
    42181 atgacaccca atgtcttact tgtgcgtgct cggattgctt gggccaaatc catttgggtc
    42241 acgattccta attttgctgc catatactgt aaaagcatag caatcagact agacatcaaa
    42301 ataactgaca tcaataaata ttggaaattt tgtccaccag tgatcgaagt ggaccagtta
    42361 cctggatcca tataaccaac tgctactaat gcacctggtc cagagtaagc aagaagtgtt
    42421 ttaaaaaaac ttttcccctt tggtacatca actgttccat ttacttcagc taaagataaa
    42481 ccatttgtat gttcaataag tcgatgctta tgaaattgtt tgttggattg attattcaag
    42541 aaaaaaccac ctttcctatt tatctacgat tacattctac acctcaaaaa gaaaaaatta
    42601 aagaaaaata ttcggtatgc ctaatttatt ttcgtggcgc gtcttgttag atgacgaaag
    42661 aaattttgtt ctcctctaaa aaaaccacta ttttttgcat gaaactaaag aatacgcctt
    42721 tcttttttca ataaacttta gtaaaataag tcattgagag caattcggaa atagaaagga
    42781 gagatttata tgttatcaac aggtattgtc gtacttattg ctattattgc tttgctaatc
    42841 ggcgcagtag gtggcttttt cctagcgcgt aaatatatga aagactattt agaaaaaaat
    42901 cctcccgtta atgaggacat gttgcgatca atgatgatgt caatgggaca aaaaccatct
    42961 gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa ataggttctg
    43021 acccaacgac tttttacggg ctgatttttt cagtccgttt tttattttgt aatttgttag
    43081 actattcaga ttattcagca aaaagacaag cacagacaat cctaggctct tgataaagac
    43141 aaaggaggag taaaatgtcg atttttagaa agttaggctg gttttttcgt caggaaaaga
    43201 aacattattt aattggggtt ttctcgctat tcgcagtggc cttggtacag ttagttcctc
    43261 caaaagtgat tgggatcatc attgatgaga ttgccgataa aaatttatca gtaaagatca
    43321 ttttcgggtg gatcttccta ttgattatcg ctgctttctt acaatatctt tttcgttata
    43381 tctggcggac gaatatttgg ggaagtgcag ctcggttgga gaaagaactt cggcagcagt
    43441 tattcaaaca ttttacaaaa atggacaatc tcttttacca aaaatatcgg actggggatt
    43501 tgatggctca tgcaacgaat gatttgaatg cgatccagaa tgtagcagga gctggtatct
    43561 tgacctttgc agattcagtg attacgggcg gaacaacgat cattgtgatg attttgttta
    43621 ttgattggcg cttaacactg attgcgctga ttcctctgcc tttgctggcc gtcacttcac
    43681 gagtgctggg gtcgaaactc catgatgctt ttcgtgattc gcaagaagct ttttctagta
    43741 tcaatgataa gacacaagaa agtatcactg ggatcaaagt cattaaaaca tttggacaag
    43801 aaaaagaaga tctagcggat tttgaagaaa agattgaaga tgcaattatt aaaaacaaac
    43861 gagtcaattt tctagactcc ttatttgatc cattcattac cttgattatc ggtctgtctt
    43921 atgttgcaac aattatttta ggtggaaagt atgtcttgga aggcacgatc tcgatcgggc
    43981 agatggtctc atttattagt tatatcggta tgcttgtttg gccgatgttt gcgattggtc
    44041 gattgttcaa cgtattggaa agaggaaatg cttcttatga tcgcgtcaat gaacttttgc
    44101 atgaaaaaac gcatatcgtg gaacaactgg gtgcagtgca gaccccggta aaaggcgagc
    44161 tgaatgtagc tatttcctct tttaattatc cgaaagaaga atcaccagca ttagaacaaa
    44221 tcaagtttgc aatccaagaa ggagaaacat taggtattgt tgggaaaact ggtgcgggaa
    44281 aaacaacgat catcaaatta ctgatgcgtg aatacgatga ttatcaaggg aaaatccaat
    44341 ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt attaggatca atcggctatg
    44401 tgcctcagga tcacttcttg ttttcgatga ccattcgaga taatatccgt ttttctaatc
    44461 caaaactttc agaagaagaa gtagagcatg cggcttcttt agcatttatc aacaatgaaa
    44521 tcaaggaatt accaaaaggt tatgatacct tagttggaga acgtggagtc tctttatctg
    44581 gcggacagaa acaaaggatt tcgattgctc gagcattgat caaaaatcct gaattattaa
    44641 tattagatga tgcactttct gcagtagatg ccaaaacaga agaagctatc ttgcataatt
    44701 taaaagaaat aagaaaagag aaaacaacta tcattgcagc tcaccgttta agtagcgtga
    44761 tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat cattgaacga ggaacccatc
    44821 atgagctgct tgcactaggc ggctggtatg aacaaatgtg ggagaaacag caattagaag
    44881 cgaagataga aggaggcgag gcctgatgga tgaaaattat caatcggaat ggacaaaatc
    44941 gattccactt aaagaacaga aaaagatcat caaacgactg atgaagttcg ccaaaccttt
    45001 tagaacaacg ttcatcgttg ctatcttatt tgcgtttgct ctgtctgtga tcaatgtcct
    45061 attgccaagg atcatccaaa cttttatgga tgatcatctg gctaaacagt cggctacgac
    45121 acaagttatt ttgttttttg ctggtatgta cttatttgga gtcctagtaa aaagtatcat
    45181 ttggtttttc caatggtatt tgtattccat ggcttcattg aagacctatc agtatattag
    45241 ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt tattttgatc agacaccggc
    45301 aggatcaacc gtttctcgag tgacgaatga cacagaaacg ctatttgaat tttggtacgt
    45361 ctttttgatg gtattgactg ggatctttgc agtcgtttct tcattcattg ccatgtttca
    45421 aatcaatgga gaaattgcct tgtggacttt ggtgttcttg ccgattttag gaattgtcat
    45481 ctggtattac cagaaattta gttctcgtat ttaccgaaat atgcgggaaa aattaagtca
    45541 gctgaataca aaattgaatg aatcgatttc cggcatgcgt attatccagc agtttcgcca
    45601 agaagctcgt ttagctaaag aatttgaaca agtgaacaat gagtatttag acacgcgcta
    45661 tgcgatgatc aaaacgaatt cgattctgtt aagtccgatc atcaattttc tatatgcatt
    45721 ggcgattgcg ttggcgttga ctttatttgg gatcgatgcg ttgcattctc ccgtagaagt
    45781 tggactgatc tatgcattta cgacttatgt ccagggattt tttaatccaa tgtctcaaat
    45841 gatggacttt ttgagcatct tcacagatgg gatggttgct ggtagtcgta tcttgaagat
    45901 tatggatacg gaagaatatg caccacaaca aaaccctgga gcagatcaaa tgattacttc
    45961 aggtaagatt gagtttcgca atgtaagctt ttcttatgat ggagagcaca atgtattaaa
    46021 caatatctct tttgttgcta atccgggaga aaccgttgca ttagtgggac acaccggaag
    46081 tgggaaaagc tcgatcatca atgtgttgat gcggttttac gaattttatg aaggtcagat
    46141 attgattgat gatcatgaca ttcgagaata tccaatggaa gagttacgta agaaaatggg
    46201 gctggtcttg caagatgctt tcttgtttta tggggatatc gcaggaaata ttcggatgat
    46261 gaatccagct atcacagacg aacagatacg ccatgcggct gagtttgttc aagcaaacaa
    46321 attcatcgat gaattaccaa aaggatatca ctcaaaagtg atcgaacatg gtgccagcta
    46381 ttctagtgga caacgacagc tgatcacttt tgcgcgaaca atcgtgactg atcctaaaat
    46441 tctagtattg gatgaagcaa cagcaaatat cgatactgaa acagaaggat tgatccaaga
    46501 aggattggcg aaaatgcgac aaggacggac aacgattgcg attgcccatc ggctgtcaac
    46561 gattcgagat gcagaattga ttttagtttt agacaaaggt catatagtag aacgcgggaa
    46621 tcatgaaaca cttctagcgc aaaatggttt gtatgcagat atgtacaaac tgcaaagtag
    46681 tggtatataa aagatgttgt gacagtagca ttttgaccta agcactagag tgaaaataaa
    46741 acatagcttc ccatattttt catctgtgag agttagtgtc gtagttacca gatttaaaaa
    46801 agacgacgtc agctagtcag aagtgcctaa tagcccctaa gaaagcgttg acaaaatcgt
    46861 ttgctttcga taatataaag aaaacgatac aacttggata gtgattgcct gatgcaagct
    46921 aaacctgatg agtctcccat ggtttcatgg acggcttttt gggtttttct ttttgttttg
    46981 gatacagccc tatatatcat ggaaaggagc aaaacatgct gattgagaaa gtactgaata
    47041 acaacgtcgt aataacaagg gatgaaagag atcgggaaat gattgtgatg ggaaaaggat
    47101 tggcattcaa aaagaaggtg ggtgacccga ttcttgactc atgcatcgac aaaaaatttc
    47161 agttgacaaa tgacggactt tcacaacagt tccaagaatt ggcacaagcg attcctcttg
    47221 agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctctg caagtccatt
    47281 taaatgattc tgtgtatatt tcattgacgg atcacttgta tacagctatc gaacgtcaaa
    47341 aaatggggat ctttgtgcca aatgtattgc tgtatgatat caaacaactg tttcctaaag
    47401 agtatgccat tgggaaaaaa acagtgaaga aaatctcgga taaataccac atcaagttat
    47461 cagaagacga agccggattt atcgcattgc atttagtcaa tgcacaaggc attcaaagcc
    47521 agcaagacaa tacctacctg tttaccacaa caatacagga tattttaaat atcgtccgct
    47581 attactataa gattacattt gatgaagatt cagtccactt ttttcgattc gtcacacacc
    47641 tgcgtttttt tctgacacgt gtcctagatg gagcgaaatc agaagatgga gaagtttctg
    47701 aggaattgtt ccaagttatt caaaaaaaat ataaggatac gtttggttgc gttgagaaaa
    47761 tagcagactt tttaaccggg gctttcgact accagatgtc cacggatgaa aaaatgtatt
    47821 taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaata aaaaaggagg
    47881 acaaattaac aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac
    47941 taattaattg gagggaatag aaatgggaaa ataccaggaa ttagcagaaa acatcgtaaa
    48001 gaatatcgga ggaaaagaaa atatccgtag cttgacacac tgtgtgacgc gacttcgttt
    48061 caaactaaag gaggaaaata aagcaaagga tgatgttttg aaaaagatgg aagggatagt
    48121 caccatcatg aaaagcggcg ggcaatatca agttgtaatc gggaatcatg tgccagatgt
    48181 ctatgcagaa gtactgacag tagccgggat ttccgatgaa gcagaagaag aggtttcttc
    48241 aagtagtaat ctgttcaatc gattaatcga catattaagt ggctgtttcc agcctttttt
    48301 aggtgctttg gctgcagcgg gaatggtcaa aggattaaat gctttgttct tgtttctcaa
    48361 attttatact gaaggatcgg gtacgtacat catgctgaat gggatagggg atgcaatttt
    48421 ttatttcatg ccagtgatct tagggtacac agcagcgaaa aaatttcatc tgaatccgat
    48481 ggtggggctc gtgattggtg cagcactttg ttatccgact gtacaaggag cagcactaca
    48541 aagtggtttc cctgatggca gtcctgcgcc ttatagcttg ctgggtcttc cggcttatga
    48601 aacatttttg ggtatcccat gggtgggcag cagttatact agtagcgtcg tacctgttat
    48661 cttcattatc gcatttgccg gacaagtcca aaaaattgcc aagaaatgga ttcctgcagt
    48721 cgtccagacc tttttggttt ctttttctgt gttattaatc gcacttcctg ctggcttttt
    48781 gattatcggt ccgattgtca gtatactgac agatctatta agcacgggat tccaagcatt
    48841 gatggacttt tctccagctg tgtatggtgc agttctaggg tttctttggc aagtactggt
    48901 gattttcgga cttcattgga gtattatccc tcttgcgatt atgcagctag cgcaaaatgg
    48961 gtggagtcag atgttagtag gcgtttatgg cgcgagtttt gcacagacag cagtggtatt
    49021 ggcgatgtat ttcaagttca aggatcaaaa aatgaaggcg ctttgcatgc cagctgtgat
    49081 ttcaggaatc ttcggcgtaa cagaaccggc tatttatgga atcaccttac cgaagaagaa
    49141 gccatttatt tactcgatga ttggtggtgc tgtttcagga atctatttga tggcatctgg
    49201 aacaatcagc tacataatgg gcggattagg gatttttggg gtggtaaact acatcacgga
    49261 aacagacgcc agtggtatgt ttcaagcgtt tatcggcatt gctattgctt cggtaatcgg
    49321 gttttcgctg acttttttct tttggaagga tgaagcaggt gaaacagatg ctatacagaa
    49381 aaaatcaggt aaagaaattg taaaaacacc gatacaagga caaatcgctc cactaaatgc
    49441 agcgaaggat gcagcttttg cacaaggaac actaggtcga ggaatcttaa tctaccctga
    49501 aaaaggagaa gtacgtgccc cttttgacgg aacagttatg accctgttcc cgacgaaaca
    49561 tgcaatcgga atggtttctg aaacagggct ggaattgctg attcatgtcg gattggatac
    49621 tgtgcagttg gaagggaaat attttgaaag cttagtgcag caaggagaaa gagtgagaca
    49681 aggcgatctg ttgctacgct tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga
    49741 aacacctgtg atcgtcacca attacacgga ttatttggat attttggaaa accaagggga
    49801 gacggtcggg ccagacgatg cacttatcac cgcactgacc tgaacggtta ggtgaagaat
    49861 ggactaggct taactattct ttttattcca cttctttatt ttcaaaattg aaaggctctt
    49921 tcatgatggc tgagctgata gggcgtttat cgaatttttg accgacagaa attagatgga
    49981 ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac
    50041 aaattgatgt attgatcgta aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa
    50101 gtcgcaaagc ggctgtactc attgcagata gcgttgaatc accgatactg gtcaaagcta
    50161 aaattggaac atcattttgt ttcaatattt ctgcggtacg aaggatcaca ttgttttcac
    50221 ccgtataaga aatcagaatc ccgcacatat cgctcgtaca attgtacgct tcatagaatg
    50281 attcaccaaa agtggtgaca attgtgacat ccattttgat gcgcttcatt ttcagcatga
    50341 aatcttgaga aatcaaagta ttcgagttac tggcgaagat cacgattttt tttgcctgga
    50401 gcagtaaata tttagctttt tgcagttggt catgatgaat gagagaaagt gtatcatgaa
    50461 tcgtcatctg ttctaaagaa gccattttat ttgcgattgt cataataccg tctgtggtgg
    50521 aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta
    50581 aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct acacggatga
    50641 gtgtagacgg gtggacataa catcgctcag ctatcgcttg gatggtcatt tcgccaagtg
    50701 ctggagaatc gcttaagaga aactggatca cttcattttc tgaaggagaa aaggagattt
    50761 ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttattttttt acgtaaatta
    50821 taactttttt gaaaatgttt tgtaaacgga tttatactat ataacttaaa ataggctttt
    50881 tttgacaaga tatttacaaa aatccactag aaaacagtaa cttttgtaaa tgttactgga
    50941 aagaaacgga tttctattgt ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag
    51001 taaaggagtg agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt
    51061 tacacccctg agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa
    51121 atctggctcg tagatgtaga agaaggaaaa gaaaagcttg agatcgttag tcagatgtct
    51181 cagcgtatgt gggatgcttc tccatatgaa gtcaagatct atgcaactct ggatcgggaa
    51241 gaagcattga aagatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca
    51301 cgaatcaaag acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt
    51361 gctggcggga tattcaaagc ttttcgtacc gttccagtcg ttttggcaat cgttgaggac
    51421 atgaagcgtt tatgtccaaa tgcttggctg atcaatttcg cgaacccaag tgggatggtg
    51481 acagaagcag ttatcagata tggcaaatgg gataaagtga ttggattatg caatgtgcca
    51541 gtgatggcga tgatgacaga accagcaatg cttggcaaga aaaaagaaga gctgatctac
    51601 aagtttgccg gtctaaatca tttccattgg cacaaagtag cagataataa aggaaatgac
    51661 cgcacggcag aattgattga tcgtttatac gatgaagata atggattacc gaaaaatatc
    51721 tttgatatcc cgtttttcaa agaacaatta aaacaaatga acatgatccc ttgtggctac
    51781 caccgctatt actatcgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg
    51841 attggtacaa gagcgcagca agtgaaagaa acagaagcag agctatttga attatacaaa
    51901 gatccagaat tagaccataa acctgaacaa ttgcaaaaac gaggtggcgc ttattattca
    51961 gacgctgcct gtgaaacgat tgcttcaatc tatggaaata aagaaacaca gatcgttgta
    52021 tctacaaaga atgatggagc tgttcctgac ctgtcggcag attgtgttgt agaagtcact
    52081 gcctatgttg gcgcacaagg agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa
    52141 agaggctggt tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agctgtgact
    52201 ggtgattatg ggacagcttt acaggcgttt acgatcaatc cacttatacg atcaggaaag
    52261 acagcaaaac ggatcatgga tgagttgttt attgcacacc agcagtattt accgcaattc
    52321 aaggaaacaa tcagccgatt aaaagaagaa ggaatcgaag ttcaagatga actggcaaga
    52381 gaattatcag gtaatacaca tataatggag gaagtataat ggggttcaga aaagattttt
    52441 tatggggcgg tgctacagca gccaatcaat gtgagggcgc ttatgatgaa ggtggacgcg
    52501 gactagcaaa cgtagatgtt gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa
    52561 agaaaaaaat gtttgaattt gatgaggatc atttttatcc agcaaaaatt gcaatcgata
    52621 tgtatcatcg ttataaagaa gatattgctt tgtttgcaga aatggggttc aagacttatc
    52681 gaatgtctat cgcctggagt cgtatttttc caatggggga tgagaaagag ccgaatgaag
    52741 ctggcttgaa attttatgag gatgtcttta aagaatgcca aaagcacgga attgagccac
    52801 ttgtgacgat tacgcatttt gattgtccaa tgcatttgat caaagaatat ggtgcgtggc
    52861 gttccagaga actcgttggt ttttatgaaa atctgtgtcg agtcatcttc aatcgctata
    52921 aaggacttgt caaatattgg ctgacattca atgagatcaa tatgatcctt catgcaccat
    52981 ttatgggcgc agggctgtat tttgaagaag gagaaaatga agaacaaatc aaatatcaag
    53041 cggcacatca tgagttggtt gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc
    53101 cggagaacaa agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc
    53161 ctgaagatgt ttgggcttct agaaaatcag atcgagaaaa ttatttcttc attgatgtcc
    53221 agtcacgagg agaatatccc gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga
    53281 tcgaaatgga accaggggat gccgcattgc tgaaagaaca tactgttgat tttatctctt
    53341 tttcttatta ttcttcacga gtttcgacta ccgacaacga actcttggaa cagactgcag
    53401 gaaatatctt tgcttctgtc aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa
    53461 ttgatccatt aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccat
    53521 tatttattgt agaaaacggc ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg
    53581 aagatgatta ccgaatcgat tacctggcag cgcatatcca ggcgatgaag gatgcagtag
    53641 aactagacgg agtcgatcta ttaggctata cgacttgggg atgtatcgat ttagtatcag
    53701 ctggtaccgg tgagatgaag aaaaggtacg gattcattta tgtcgatcgt gataaccaag
    53761 gaaatggcac attagcccgt tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg
    53821 attcaaatgg agaaaatctt tcgaacaatt gatcaatgat ttataaaagt ctattgtttt
    53881 tcagcggatt tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt
    53941 tttttcgcat tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt
    54001 atgatgtaat cgttattggc gcaggtcctg ctgggatgac tgctgctttg tatgcttcac
    54061 gttcaaactt atccgtattg atgatcgaac aaggtgcccc tggcggccaa atgaacaata
    54121 cggcagaaat cgaaaattat cctggattcg attcgatcat gggccccgat ctagcttata
    54181 agatgtatga aagtgtctca agatttggga cagaaaatgc ttatggaatc gttcaagata
    54241 tcaaggatca cggcagccat aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa
    54301 cagtcattat cgcaacaggc tgtatccacc gtaaattagg cgtacctggc gaggaagaat
    54361 ttgctggacg aggcgtttct tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac
    54421 gtctagtagt catcgggggt ggggactcag cagtagaaga agccatctat ttgacacaat
    54481 ttgcttctga agtagtgatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc
    54541 aagaccgtgc atttgcaaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa
    54601 tcgttggaaa cgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg
    54661 aatttgattt agcagcagac ggagtattca tctatgttgg actggatcca ttaactgcac
    54721 cttttagaga agcaggtttg acgaacgaag ctggctggat tccaacagac aacgaaatga
    54781 aaacgaatat gccaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa
    54841 tcactacagc tgttggtgac ggcggtattg ctggacaaca agtgtataaa tacttggagt
    54901 ctttagaaga aacaacagca taagtttaaa aagagtgaaa gaacggtttc aattgttctt
    54961 ttactctttt ttttcgcgtg aaatccactg aatccgattt atgtgaatac ttgcaatcat
    55021 gcttgataaa agaatccttt cttgatttaa gcgttttttg gtcctcatac tttcatatct
    55081 aataagtgaa tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt
    55141 cttgggaaca agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa
    55201 aacaattaaa tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt
    55261 tggaattcgg tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca
    55321 tctatacggt aagacaagca acagaaggac tagcacgttt catggataca caaggtgaag
    55381 aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg ccatcagtca ccagaatttg
    55441 caatggaagc agccaaaaca ctggcacatc acaatatccc gtcttatgta ttcgaaagtc
    55501 tgcgtccaac accagagctt tcttttgcag ttcgtcattt gaagacgttt gcgggaatca
    55561 tggtaaccgc atctcacaac ccagcggctt acaatggata taaagtctac ggagaagatg
    55621 gcggacaaat gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca
    55681 atccgctaaa agtcgaagta ttatctgacg aagaagctaa acatagcggg ttgatcacga
    55741 tcatcggaga agaagtggac gctgcttatc tagaagaaat caaagcagtg acgattgatc
    55801 gtgaattggt tgaaacaatg gggaaagatt tgaaacttgt gtacacgcca cttcatggaa
    55861 cagggaaaat gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag
    55921 ttcccgaaca agctgtggca gatccagatt tcactacggt caaatcgcca aatccagaag
    55981 aacattcagc ttttgaatat gctatacgac ttggagaaaa agagggagct gacttgctga
    56041 ttgcaacaga tcctgatgca gaccgtttag gcgcagcggt tcgtttgcca gatggttctt
    56101 atgaagtatt gactgggaac caaatcggag cattgtttgc tcaatatatt ttggaagcac
    56161 atcaatcaag aggcacttta ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg
    56221 aattgcctac agcaattgcg gaacactacg gtgctactat gttcaatgtg ttgacagggt
    56281 ttaaattcat tgctgaaaaa atccaacaat ttgaagaaga ccattcacat acttttatgt
    56341 ttggattcga agaaagttat ggttatttag ttaaaccttt tgttcgcgat aaagacgcga
    56401 tccaagcatt gcttttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt
    56461 tgtatgacgg cttgcaaacg atttttgaaa cctatggcta tttcgcagaa aaaacgattt
    56521 ctgtgacaat gagcggacaa gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc
    56581 gtaaagaagc accgacagaa tttgccggag caaaagttat tcaaacagaa gactttaaag
    56641 aattgacgag aacagaccgt gaaggaaacg tagaaaaact aacaacaccg ccatccgacg
    56701 tattgaagta tacattggaa gaaaatggct ggatcgctat ccgcccttca ggaactgagc
    56761 cgaaaatcaa gttttatatt ggtgtaaaag gaagctctac agaagacgca gccgaaaaag
    56821 tagcctctta tgaagcgatt atcaaaaaga tgacagaaga ataaaaaact tgagactgat
    56881 ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc
    56941 agacacgcaa aaatcaaaag tagagaaagt cagtcaatta ttcaaaatgt tgagtgaccc
    57001 cactcgtctt aaaattctgc tatatctgaa agacggggaa caaaatgtga cagcaatcac
    57061 ccaagcagtg gaaatggagc agtctgctgt atcgcatcaa ctacgtttat taagagaaaa
    57121 tcatgtcgtc aagtctcatc gcgagggaaa agcgattttg tatagtcttg atgattctca
    57181 tgtactggat attttgaacc agacactcaa acatgtggaa catcaataac gataggtgc
//
LOCUS       DAZAJZ010000007        50725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000007 DAZAJZ010000000
VERSION     DAZAJZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.007"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG86D_000450"
     CDS             <1..1412
                     /locus_tag="ACG86D_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN3139628.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACG86D_000451"
     CDS             1546..2889
                     /locus_tag="ACG86D_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3139629.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..4452
                     /locus_tag="ACG86D_000452"
     CDS             3001..4452
                     /locus_tag="ACG86D_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3139630.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4801..5433
                     /locus_tag="ACG86D_000453"
     CDS             4801..5433
                     /locus_tag="ACG86D_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN3139631.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6021..7460
                     /locus_tag="ACG86D_000454"
     CDS             6021..7460
                     /locus_tag="ACG86D_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3139632.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7755..7973
                     /locus_tag="ACG86D_000455"
                     /pseudo
     CDS             7755..7973
                     /locus_tag="ACG86D_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            8222..8437
                     /locus_tag="ACG86D_000456"
     CDS             8222..8437
                     /locus_tag="ACG86D_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139633.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8654..8910)
                     /locus_tag="ACG86D_000457"
                     /pseudo
     CDS             complement(8654..8910)
                     /locus_tag="ACG86D_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9097..10008)
                     /locus_tag="ACG86D_000458"
     CDS             complement(9097..10008)
                     /locus_tag="ACG86D_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3139634.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10027..11031)
                     /locus_tag="ACG86D_000459"
     CDS             complement(10027..11031)
                     /locus_tag="ACG86D_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN3139635.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11028..13151)
                     /locus_tag="ACG86D_000460"
     CDS             complement(11028..13151)
                     /locus_tag="ACG86D_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN3139636.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13156..15603)
                     /locus_tag="ACG86D_000461"
     CDS             complement(13156..15603)
                     /locus_tag="ACG86D_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3139637.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15590..15979)
                     /locus_tag="ACG86D_000462"
     CDS             complement(15590..15979)
                     /locus_tag="ACG86D_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3139638.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(16039..16542)
                     /locus_tag="ACG86D_000463"
     CDS             complement(16039..16542)
                     /locus_tag="ACG86D_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN3139639.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16555..16779)
                     /locus_tag="ACG86D_000464"
     CDS             complement(16555..16779)
                     /locus_tag="ACG86D_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139640.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16883..18793)
                     /gene="ltrA"
                     /locus_tag="ACG86D_000465"
     CDS             complement(16883..18793)
                     /gene="ltrA"
                     /locus_tag="ACG86D_000465"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN3139641.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19539..19676)
                     /locus_tag="ACG86D_000466"
     CDS             complement(19539..19676)
                     /locus_tag="ACG86D_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN3139642.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(19673..20857)
                     /gene="mobT"
                     /locus_tag="ACG86D_000467"
     CDS             complement(19673..20857)
                     /gene="mobT"
                     /locus_tag="ACG86D_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN3139643.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(21116..21406)
                     /locus_tag="ACG86D_000468"
     CDS             complement(21116..21406)
                     /locus_tag="ACG86D_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139644.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(21396..22538)
                     /gene="dcm"
                     /locus_tag="ACG86D_000469"
                     /pseudo
     CDS             complement(21396..22538)
                     /gene="dcm"
                     /locus_tag="ACG86D_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22614..22778)
                     /locus_tag="ACG86D_000470"
     CDS             complement(22614..22778)
                     /locus_tag="ACG86D_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139645.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(22771..23445)
                     /locus_tag="ACG86D_000471"
     CDS             complement(22771..23445)
                     /locus_tag="ACG86D_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3139646.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(23523..24863)
                     /locus_tag="ACG86D_000472"
     CDS             complement(23523..24863)
                     /locus_tag="ACG86D_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN3139647.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(24946..25281)
                     /locus_tag="ACG86D_000473"
     CDS             complement(24946..25281)
                     /locus_tag="ACG86D_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN3139648.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(25291..25665)
                     /locus_tag="ACG86D_000474"
     CDS             complement(25291..25665)
                     /locus_tag="ACG86D_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3139649.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(25678..25992)
                     /locus_tag="ACG86D_000475"
     CDS             complement(25678..25992)
                     /locus_tag="ACG86D_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3139650.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(26105..29332)
                     /gene="fss3"
                     /locus_tag="ACG86D_000476"
     CDS             complement(26105..29332)
                     /gene="fss3"
                     /locus_tag="ACG86D_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN3139651.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            30189..30443
                     /locus_tag="ACG86D_000477"
     CDS             30189..30443
                     /locus_tag="ACG86D_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139652.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            30607..31524
                     /locus_tag="ACG86D_000478"
     CDS             30607..31524
                     /locus_tag="ACG86D_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3139653.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            31517..32299
                     /locus_tag="ACG86D_000479"
     CDS             31517..32299
                     /locus_tag="ACG86D_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3139654.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            32300..32980
                     /locus_tag="ACG86D_000480"
     CDS             32300..32980
                     /locus_tag="ACG86D_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3139655.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            32973..33869
                     /locus_tag="ACG86D_000481"
     CDS             32973..33869
                     /locus_tag="ACG86D_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3139656.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            34081..36003
                     /locus_tag="ACG86D_000482"
     CDS             34081..36003
                     /locus_tag="ACG86D_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3139657.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            36004..36450
                     /locus_tag="ACG86D_000483"
     CDS             36004..36450
                     /locus_tag="ACG86D_000483"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN3139658.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            36466..36780
                     /locus_tag="ACG86D_000484"
     CDS             36466..36780
                     /locus_tag="ACG86D_000484"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFN3139659.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            36806..37855
                     /locus_tag="ACG86D_000485"
     CDS             36806..37855
                     /locus_tag="ACG86D_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN3139660.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            37867..40470
                     /locus_tag="ACG86D_000486"
     CDS             37867..40470
                     /locus_tag="ACG86D_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN3139661.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            40587..42236
                     /locus_tag="ACG86D_000487"
     CDS             40587..42236
                     /locus_tag="ACG86D_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN3139662.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            42552..43487
                     /locus_tag="ACG86D_000488"
     CDS             42552..43487
                     /locus_tag="ACG86D_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139663.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            43480..44250
                     /locus_tag="ACG86D_000489"
     CDS             43480..44250
                     /locus_tag="ACG86D_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139664.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            44665..45648
                     /locus_tag="ACG86D_000490"
     CDS             44665..45648
                     /locus_tag="ACG86D_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN3139665.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            45969..47006
                     /locus_tag="ACG86D_000491"
     CDS             45969..47006
                     /locus_tag="ACG86D_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3139666.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(47095..47418)
                     /locus_tag="ACG86D_000492"
     CDS             complement(47095..47418)
                     /locus_tag="ACG86D_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN3139667.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(47405..48112)
                     /locus_tag="ACG86D_000493"
     CDS             complement(47405..48112)
                     /locus_tag="ACG86D_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN3139668.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            complement(48411..49835)
                     /locus_tag="ACG86D_000494"
     CDS             complement(48411..49835)
                     /locus_tag="ACG86D_000494"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3139669.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            50010..50723
                     /locus_tag="ACG86D_000495"
     CDS             50010..50723
                     /locus_tag="ACG86D_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3139670.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT        15899 a         9466 c         9570 g        15790 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
     3301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
     3361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
     3421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
     3481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
     3541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
     3601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
     3661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
     3721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
     3781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
     3841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
     3901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
     3961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     4021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     4081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     4141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     4201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     4261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     4321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     4381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     4441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     4501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     4561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     4621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     4681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     4741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     4801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     4861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     4921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     4981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     5041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     5101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     5161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     5221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     5281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     5341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     5401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     5461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     5521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     5581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     5641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     5701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     5761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     5821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     5881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     5941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     6001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     6061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     6121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     6181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     6241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     6301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     6361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     6421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     6481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     6541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     6601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     6661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     6721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     6781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     6841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     6901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     6961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     7021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     7081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     7141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     7201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     7261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     7321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     7381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     7441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     7501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     7561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     7621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     7681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     7741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     7801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     7861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     7921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     7981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     8041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     8101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     8161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     8221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     8281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     8341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     8401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     8461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     8521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     8581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     8641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     8701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     8761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     8821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     8881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     8941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     9001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     9061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     9121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     9181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     9241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     9301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     9361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     9421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     9481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     9541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     9601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     9661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     9721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     9781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     9841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     9901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     9961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
    10021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
    10081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
    10141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
    10201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
    10261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
    10321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
    10381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
    10441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
    10501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
    10561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
    10621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
    10681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
    10741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
    10801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
    10861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
    10921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
    10981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
    11041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
    11101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
    11161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
    11221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
    11281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
    11341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
    11401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
    11461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
    11521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
    11581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
    11641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
    11701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
    11761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
    11821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
    11881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
    11941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
    12001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
    12061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
    12121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
    12181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
    12241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
    12301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
    12361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
    12421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
    12481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
    12541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
    12601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
    12661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
    12721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
    12781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
    12841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
    12901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
    12961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    13021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    13081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    13141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    13201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    13261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    13321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    13381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    13441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    13501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    13561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    13621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    13681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    13741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    13801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    13861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    13921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    13981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    14041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    14101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    14161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    14221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    14281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    14341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    14401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    14461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    14521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    14581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    14641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    14701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    14761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    14821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    14881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    14941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    15001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    15061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    15121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    15181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    15241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    15301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    15361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    15421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    15481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    15541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    15601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    15661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    15721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    15781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    15841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    15901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    15961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    16021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    16081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    16141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    16201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    16261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    16321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    16381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    16441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    16501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    16561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    16621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    16681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    16741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    16801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    16861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    16921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    16981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    17041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    17101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    17161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    17221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    17281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    17341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    17401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    17461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    17521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    17581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    17641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    17701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    17761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    17821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    17881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    17941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    18001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    18061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    18121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    18181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    18241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    18301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    18361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    18421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    18481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    18541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    18601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    18661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    18721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    18781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    18841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    18901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    18961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    19021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    19081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    19141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    19201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    19261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    19321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    19381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    19441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt ttcactaagg
    19501 aacgagtcgc acataaattt tgtccgaaat tcatcttgtc actttccttt cttttccatt
    19561 gctcgatccg ctgctgcact tgcattcatc aagcacatca cagtaacacc aagcatactg
    19621 ccaccaaata ccaaaaaaat atcttttaca attcccatga cgattcctct tttcatataa
    19681 tcaaattttc aattgccata ctttgttgct ccaaaatctt tttatgtcgg tctgataagc
    19741 gtgcattctg taccatatcc tggatatagg ttgtccgatt caatcgatcc aactccttag
    19801 ccattttcca cgtaggggcg acttgtcggc ctagccaatt caaggttcgt tcaatggtat
    19861 acggttttgg ctcggaagtt aattgaatct ctttccgatt ccgtccaata aaacgccccc
    19921 attgttcatt caagggccag ttggttcgcc ttttaccttc tactttatcc gcaaatcgca
    19981 agtaacgatt gataatggaa aacgtggtgc tctcaatgct acgacctttt aacaaatctt
    20041 gaatcgcatg aaaagcacgg tcatttttca gtcgaatctc aaaacgattt ttcgtctcag
    20101 tttggtctaa tggaatccct aacttgatat attgttcata gtctttttca tacaaacaga
    20161 aataaacctc actcttgaga ctgccgatat ataaggtatt gcccatgccg tccttttcat
    20221 cagcctttaa aagttccccg gaacgataac ttttaaaggt acgaaacaag gagatacatt
    20281 cttccttctg acattttttc gttagctcag gtatatccaa tagccccact cgatcattga
    20341 ttgccaagtc caagcgtttc atcacgccac cggcctttag acaatcctcg aaaaagtcgt
    20401 accagctacg cttttgagcg agtaaaaaag tttcaaattg tcggcaacct cgccccttta
    20461 attctaaaag cacaccttta tcttcttcat gagacaacat caccacaata tcacccatga
    20521 catactgttc ttgataggag taaaaggcat aatcttcatg gagcatatac tcaaatttca
    20581 aatggagaat ttcttgaata atttttcgtg catccgttgt gggaaagcga atccttacat
    20641 agtcaaacag taacgtcaaa ggcaaatcgg gattaaagct ttccagttgg tcaaaaattt
    20701 tcctcatggt ttttatagat gcggttacgc caccattttc tatctgattt aagtacggcc
    20761 gactaagtcc acaagccgta gccaatttat tttgtgaaac gccgtattcg ttgcgtcggt
    20821 cttttaatag ccgataatct aaattccgtt gtgccaagtt ctcacctcca aattcaaggt
    20881 gttaactttt tcaaatgagt taacacctat acaaattact tactatcttt gatacaacaa
    20941 gcattcttaa tggttgctta cttatttttt gtgtttttgt acccccctgt tagatacggg
    21001 ggtgagacat catggtcggc taacgccgtc catgaacagg cacagcacca cggaggattt
    21061 ctgcttcgcg aatcctccgc ggtcagcgcc taacagtcag ttgtgttggt ttaggttact
    21121 catctgtaat accatttact tccaactctg ctttcatttc taacaagtat tgacgaatat
    21181 ctggttcttt tgcttggtcc gcaatccgct caaattctgc aattacttga tttatttcaa
    21241 ttcgagcatc cgtctcctca ttcattaaat atggatcgcc agcgactttg acaccattct
    21301 tgcgaatatc aaattttaac cgtcgcaatt gttcttctaa ttctgggtct gactgaatct
    21361 ttccaatcac ccaataaatt tctccaatca actcatcata aaccatgtac atccaactct
    21421 tttaatcttc tggcaatatc aaaaatgacc ggaactgtta cagagtttcc ggcttgctta
    21481 tacaactgac tatctgaatt gacttgagaa tctcgatcaa aagcccaatc gggaaagccc
    21541 tgaagtcgcc aacactctct aggtgttagc ttccgaattt gatactgatc cgtgacgatc
    21601 gcttgttggt ctcctgttag taaggtatga acactctgtt ttcccagacg acctcggcga
    21661 cttgtggaat taggaaaagc cagattcaca ctgtcttctg gcttcacctc ttgatattct
    21721 gtttcatttt ttcgaatcat gatttttgta ccaccttgtt tcgttgacaa ggtgggggcg
    21781 gttccagtaa cttcataaac tcgattacct tgttcaaact ttcccggtaa tagtcctgct
    21841 acagagattc catcccttgc tggcagtgcg attttgattc cttcatcacc tgttaaggtt
    21901 ggcgctacgc catctgaacc atagacttgt ccacccattc ctcgattgga aggattgata
    21961 tttaccaaat ttctgatgtt ttgatcagat gttgaagacg gtccaatgat aggaaatact
    22021 tgttcggtac atgcgtctcg agaatgtccg ataaggaata tccgctcccg gctttgggga
    22081 atgtaggcct ttgagttaag cacctaccac tccacgtcat accccaatcc atccagcgtt
    22141 cggaggatcg tctcgaacgt agcccctcct tcgtgattga gcaatcctcg gacgttctca
    22201 aggaataaaa gcttaggtcg gagaagagtg gtgaaccgag cgatttcaaa gaataaagta
    22261 cctcgagtat cggaaaatct ttgccgcttt cccgcaattg aaaaagcttg gcacggaaat
    22321 ccgccgcaaa ggatgtccac tggtccgagg gattgaataa actcatctga tacacttgtg
    22381 atgtcatgca tttccacctc cttacttgta tcatgaattg ctttataact acgtctagca
    22441 aatttatcaa tttcacaaaa accgatacaa tgatggctcg cctgttccat acctaaacga
    22501 aagccaccaa taccggcgaa taaatctaaa aatctcatac attacctcat tcattgcaaa
    22561 gaaaaaagcc aagaacattt tgctcttggc tcatacatta ttaatttgtc ctactatttt
    22621 ttatttaatc caactaattt ccgttgtttt tcattggcct gatcggctac atatttaatc
    22681 accgttttga actcttttgc agtagcattt tccgataaat ccgcaatgct cttattgtat
    22741 ttctttacaa ttttcgttac ttgttctttc tcagtcatgt aattgaacct ccaaatcttt
    22801 cctactatac cctagtggta aaaataattc tagattatcc gtagcataat gaacttcgga
    22861 aatattattc gtataatctt ttattatgat atgaagcaat acatctggat gtttactctt
    22921 tacttttgca atgttctctc ttgatttgaa aaaagcgtta ataaatgcct ttttcggcac
    22981 atgacgtccc tctgcaattt cacgttcttg tgtaaatttc caagcgattt tagggtcttg
    23041 ataaacatag aaaatcgtca ctcgaaaatg ttttttcaac gcccgcgaga tatttttttc
    23101 agcacttaat attgcaaaag ttgcatccag aataaaagaa tagccatgat ctaagacata
    23161 tctaaatgcc tgctctacca accaagaaga tgctttttga aagagactag agttttttcc
    23221 attataatca ggaaactggg tgcgaaagta atccgcatca ataatcacat ggttatcata
    23281 attctcagct aatccaatgg caacctctga ttttccagcc ccaggactcc ctgccataaa
    23341 aactgcgtct cgtccaactt cgggggattt ccctttgata atataagaaa taaacttatc
    23401 cttatttgct tttgcatact ctaaataaga atcattactt tccatattaa ttatcccctt
    23461 tcttcctact atataaaagt attatacgtt acttgaagta taaagtcagt attgaagatc
    23521 actcaatcat tcatcttttc taatttacta attgaatcta agaaatcata cccttttggt
    23581 acaagtggcg tataaaactc actaatcaca ctgccacctg tatccacata acctcgacct
    23641 ttgatgtgct tcataaagaa gtttttttcg acctccccaa acatcatgga atatccgagt
    23701 tcactcatac gacccaaagc cacgcgaaag ttgaattgat cgcggattcc gtctcctaaa
    23761 tatttcgcat ccggcctttg gcaagccaaa atcagaaaga aaccagattg acgacccaac
    23821 atcacgattt gtttgagctt attcaaaatc accgcacttt ctttcgtcgt taacatctcc
    23881 atataagcca cgtattcatc aaagattaaa aagttgggtg gaagtccaag ataggcataa
    23941 ttctctcctg ttttgtagtt agacatttct ttcattgcct tgctacgagc catcatacgt
    24001 tcatagaaat cttccacaca agcagaaatt tcttcctttt gtgaataaac atgaggcatc
    24061 accgtaccta aatcagctaa atctgcattt ttgggatcga ggataaacaa ttctgcatct
    24121 gacttcaata gtgcttcgat aatggtgagg aggaaatagg tcttccctcc acccgtgcca
    24181 ccagcgatta acatatgggg taaagaatca taagcccata cttgattttt catcaatcgc
    24241 agagtcccat tttctgccac tacttcgtct atcccaattc gattggcgat catatcatac
    24301 agtaaagtat actccacata ggagtctttg agttcctttt ctactaactc acaatacagt
    24361 ccgctttcta acttcttttc caattttaaa agctgatctt ggtatttccc aagtgaaatt
    24421 tgaacgcgaa tagacagtaa tccatctttc attcgataac agattttggg gaagtaactg
    24481 atagtctctc tggttcggct actattcaaa tctttgaaaa agccttcact ttgagtttgt
    24541 ttcacttcat accaatggtt ctctagaatc atccgagcta gcttttgtcg atgccacaag
    24601 cttcgccaat gatcaagaaa aaagtgaata taaagcaata tcatagctcc tgaaaaagcg
    24661 agacttccaa gcagacatac taaatctttt atagaccaaa cgatttcaat agtagaaagt
    24721 gatagcttag aaatatcaaa ttgtctaaac tcttgaaaat aaaagacgct catccaaccg
    24781 atgaacagag cgagtagcca gccaagaaca aagtgataaa ccaagtgctg atctcctgcg
    24841 cgtatgcgat gtcctttgta cattttgatg gtttccacct ccaatcgaaa aagcccaata
    24901 ggatttcatt aggcaatttc aaaaagttat gctagtcatt tactgctacg aggaggggag
    24961 ttgtttcgtt tcaaccaatt gatttaaaaa gccttcaata gcatccggca tctccacacc
    25021 aatttcttcc aaaatggatt ctgcctgccg acataagttt tcatttactt cgacaactat
    25081 aaagaaagac tcttcttctc ctaaccagtc tagtacatca gagtcttctt cactgtcgct
    25141 attttctcca agtaaacctt gatgtataat cagaaacatt ttttcatcag aggttaatgc
    25201 tttaacgagt tgttcctcag tcagctcgcc ttttccatat tgatcgagac aatctaaaat
    25261 atgttcaaca ttaatttcca taatcttcac ctatttcttt ctttcatttt ggttcgtagg
    25321 atttccttga ttcggctgcc gcttcaagac taaatcatcc gccttaatga accaattaac
    25381 ggtagttcct tctcgaaaaa cataattggc aacggtatcc acgattggat tgaccaactc
    25441 tacctctgca tcataagcaa actcgcgtaa gggtacatta gctgggatac taacttgaat
    25501 cattcgccct tgtgcagagg attttaaatt ataggttcgt tcttttacct cattaccaat
    25561 tgttccatcc tcattttgct tccgtacttc tcggcgtaat gcggaaaaac gcaacttccc
    25621 aaatgttaaa cctgaatcta cgacaatacc ttctgttaat ctcatcttct tttcctccta
    25681 tgctttcact aaatcatcaa catacaacac gtaatcagta aacccacgat tctcaatctc
    25741 atagcctttg gcaaccaaat ggggattcac ggcttttagc ggttggtctt gatcaaaatc
    25801 cttttcaccg gcttccgcag ctactaccac ctcaatatca tcggcccgtt gcttactgga
    25861 atacaaatga taactgcgac caatcaccgt agtattccgt ccatatcctt ctgttataat
    25921 ttctccttca ccggcataac ttaactttcc aaaggtttca gccatatctg gcacgacaaa
    25981 tttcaattcc atcttctttt cctcttttca ttgttagttt tggatacaaa aatagcgcga
    26041 ctactttttt aggaggcggt cgcgcacgtt ggttcttagg atttacttgt atcatctcat
    26101 caccttagtt ccggcgattc cagaaataat agcccgccgt cgcaaaaatc aaagtaaaac
    26161 caatgaacag taaaacattg gaatttttct ccccggtttg agggaaggtc ttcactgtgg
    26221 gactagattc ttgagagcca cttggaacag ttggtgtttc cggttgtttc ggcgtactta
    26281 gtatggttgg aacttctttt ggtgttaagg tttgagattt ttctttcaaa tcttcattgt
    26341 gttttccatt gatattccca tctttatcgt aattgatttc cgcaaaagta aacttggttc
    26401 cttctggata ttttccggta tctacttttt tcgcaagcac agttttggtg aattctttat
    26461 catttacttc ataatcaatc ttgccagatt tccaaatttc tttgtttgta ccatccggta
    26521 ataaagcata cagaattgtt tcgaaagctt cttttgagcc atccagtaca tcatgagtaa
    26581 tcgacacatc atcaaacata tccaccacat caccatgagt aaaggtctga gaaccgtttt
    26641 ctaggtgggc ctttgtttgg atagaaacat ggtgttccac tgtacaactg acagtttggg
    26701 cttggttatt caagctaaca tccttcgcta ctggcttatc gcctgcttca aaagcttcct
    26761 tattttcata cacatagttg aataagacaa tagttttatt taaaacttgt tcggcggtta
    26821 atttatgctg aattttccaa gttcctgttt tctctttgtt cgccaacgtt gcgcttgttt
    26881 ctgcaatcac cactggtttg gctttttcat ctttttcttg ggcagctttt gtcgcatcca
    26941 catcaatggc ttgcgctacc acataccagt cttctttgat ttcatgtaga ttgtaactca
    27001 atttatcaac tagctcggta gaatccagag aagttaattc tttgtttccg tctttccaag
    27061 tcgcaagaga agttaaacta tcttcttcct caggcaaatc atagagcatt aaacggttca
    27121 aactaacaga aaatgctttg ttcgtcagct tctcgtaagg aacggtcacc gttttaattg
    27181 gttgtttttg gtcttgttcg gtaatggtaa agacatactc actcttcaca aagtcttctt
    27241 tgttttcctt aaaagtagaa cggatttcta atggcgtaat cttttgaaaa ccttctgggg
    27301 cttctacttc ttcaagtaaa taatccccat aaggaagatt ttcaaacttg ccataaccat
    27361 caaaccctaa ttgctcgtta taagctgtgg tcgcttcatc ttcggcacct gtgatttcat
    27421 tggtcccttc caatggcgac actttaaagg tcagatcatt gaagccagtt tcggtagtgc
    27481 ccgcagctga tccagcaaat ttaaagaaat caaagccaaa gcgaatcact tgttcttttg
    27541 ccgtaacatt tcgggtaatc acggcatttt tttcgctatc atccaccttt ttgatggata
    27601 caggatactt tgtttcatca aggctatatc cttcgggcgc ttttgtttct tgccaataat
    27661 actcgttgat agctaaatgt ttaacggcaa cttggttctt ttcgtctaaa gtcaaagtga
    27721 ccgtttcatc agaacctttc gttcccttcg ctaactctgg tttaaaagcc tcactccatt
    27781 taacagcttt tccatcctta gcagtaaaca aggtgtattc agcaccctca aagactgcct
    27841 taccttgtgc tttatcccca gtatctttgt cttctttcgt caaagtggtt tcgccagtga
    27901 cttcttgatt ttgccctttt acgttactgg taacaggggc tacggtttga ttggcatact
    27961 tcaattcgac ttttacaggt ttgaacgtat tcacgaaccc atgactagct ttagtttcgg
    28021 tcacataata ggttcccaat tccaacgcat tagcaatctc ttttggtgtt tccgcatgac
    28081 cgttttcatc cgtggtcatt tcttgaacga tttcaccggt tggactgtct ttgcgaatgg
    28141 caaacgtatt accagctaag gaataattgt cattccaaag atcactccct gtttcaactc
    28201 cgcttttatc cagaatgatt tgcccttttt cccgtgcgtt ttttgaagtc acgtaaatgg
    28261 tttcgcctgc tttaatggtg gtagtcatgg gggtggtatc aatcgtataa ggcgccggta
    28321 ctgatttttc ggtaatggtt acttttgtac catgaggaat ctcatccaat gtagcaatgc
    28381 cttctttgtc agtagtcacg tcttttgcag gtaaagtttt tccaaagtct aaatggaaaa
    28441 ccgttcctgg tacaacatca cccgattctt tatcgacttt tttgatcttt aaagaaccct
    28501 ccgtttccac atcaattttg attgtgtagg tattaggttt atcaatcgcc ccggccatta
    28561 aagtctgttg gccagccttt ttataggcga caggtgttcc ggtaccagca gatttcttaa
    28621 gcgtcagcac accactttct tttgaattgg catttggggt gattatcaac tgattaccat
    28681 tcacacgata atcaatgttt gcggcatttt ccacaacctc atcgaactct gacaaattca
    28741 gattattcgt atccgtcaca gtagtcgatt gccctaatac tgttttagcc gtactattat
    28801 ggaaggttgg tttcttctga taatcgtcaa tggcttgatt tatttttgcc tcaatggctg
    28861 cgatatttac ggcactacca tctgatcgtt ttattgagtg aagggtatac ccattaactt
    28921 cttgccagat catcttctgt gccacaatat gagtatctgc atctgtacct acttttttcc
    28981 ataaaacgga aactaatttg gctttatcgg acatatctgg taacgggttt ttctcatagc
    29041 caggagtgaa ctcattgtaa atcgggactc ctggttcaat acagaaaata tcttgtttaa
    29101 agccgttgta cactgcataa tgcggaacag tgacttctga cattggacga ccatcactaa
    29161 aagttccttc aacagcatat tgatgtgcta aatctagttc tacggttacc gtttgtggat
    29221 gggtaatttc atcagcaaaa gtaatcgtag gagcaattgc tccaacaatt gcttgaacag
    29281 ccaaactcaa aatagttaat acacataatt tctttttttg ttttttatac atgaaatgtt
    29341 ctcctttcaa attaagcaca aaaaaataag atgctcgctt gaacatcttt gtttataaag
    29401 tctttttaaa atttttattg actgaataga atggatttga gaatgaatga gcctaaaaat
    29461 ttaatctaaa atacgaaata tactgccttt aaattagaac acattttaga acacaaacat
    29521 ttcaaattta atcaaatata ataaaatata tttacgggaa aacctttata aatcaaggtt
    29581 tttcaaactt attcaatgat acgaagaaaa aaccaggtct taagaaagcc cgtaaagctt
    29641 cacagttctc aaaacgttaa ttgcgattat cgttcgagtt cacgagttac gagaatcgta
    29701 ctgggcgaag caatgctgag cgcagttacc ttaatcctaa cttttctggc actttccaca
    29761 atttggaaag tgcctttttt gtgtttttag ctatgttaat aggtaggatt cttggtctat
    29821 aatatcattc gatctataaa caaacgggaa aaagataaag tatcatcaaa caatattatt
    29881 tatccgcttc tgaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaatttaca
    29941 aaaattgctt ctcatgtttg ataaatattg tgacttgtga ccgaggagtt ggcaggagaa
    30001 caccgtttat tcggaagaag ggattgtgaa aagtcttttg tcataacctc ttatttggta
    30061 gctaattgta aatcccaaac ggcatgtatt atttttcata agtccgtttt taaggattaa
    30121 tgttttcttt tgctttttat atggtctggc ttcagcgaga agttccaacg catatacaag
    30181 gacgacttat gtcaacgata acaacgatta ccatggtggc cagtccactt ggattagcac
    30241 tgtttggtgc gctctttgat tggaagacaa cgcaaccacg tctgcgagac agtctaattt
    30301 ttctgggagg gagtcttgct atagttatcc ttataaccgg tctggtaaaa ttactcaagc
    30361 ttgatctgaa agaagcaaaa attttcagtg aagaggaata cagagaaacg actttggtcg
    30421 aaaagaaaga atcaggaaac tgatacaagc gtataacaaa ctgcctgata ataggaaaga
    30481 cacgtattgt ccgattgttt tttttgacat tcaattggaa aatttacact ttctttatac
    30541 atttctggct gctttcttta tacccgtcaa ctatacttca ctcatcaata agaaagaagg
    30601 ataagtatgg attttggaaa tgaaacggtc ttgaaagcaa ctggtataac caaaaaatac
    30661 ggtgctgcca aagcattaga taaagtatca atcgaaatca aacgtggaat gatctatgga
    30721 ttgattgggg aaaatggagc gggtaagtct acctttatga gaactattat gggtttgatc
    30781 tcgattgatg aaggttcgat cgaattattt ggaacgacgg atttgcaggc agcaagacga
    30841 cgtatgggtc aatcgattga aacccctgct ctttatccag aattaactgc aagagataac
    30901 ctacgtatcc aagcagcgaa cggcggtgtg tcggatagag aaatcgaaga cctgctgaaa
    30961 atgatgcgat tggaaaatac agggaaaaag aaagcaaaga atttctcttt aggtatgcgc
    31021 cagcgtttag ctattgcaaa tgcactgatc acgaatccag aatttttgat tttggatgaa
    31081 ccgacgaatg ggatggatcc agcagggatg gcagaaatgc gagaaatcat ccaacgtttg
    31141 gtaaaagaac ggggcatcac cgtcttgctt tctagccact tgctagatga attgtcacag
    31201 atcgccactc actatggtat tttgcatgaa ggacacttga tcaaagaatt atcgaaagaa
    31261 gaattggcac aagaatctcg tcaatttata aaaattgaca cttcggcgac ggaacaagca
    31321 gtaacggttc tcgactctct tggttatagg gattattttg tacaatctag ccgtgttatc
    31381 cagctttttg aaggaattga ccaagtagca gccatcaatc aagcattggt agaagcaaaa
    31441 gttcctgtgg atggtattca cctagttggg caaaaactgg aagattattt cttacaatta
    31501 actggaggca atccacatgt ttaatctatt aactgctgaa aaaataaaag tatggaagag
    31561 ccgtaaaatg tgggtctcac taggactgat ggtcgtactt ccgctttttt attcttggaa
    31621 cgaatggtat atgcatacaa aatacggcaa tgaattaaac caagcaacgg atacagtcat
    31681 caacggtgca acagggattt taatggtaga aaaaatggct gcatggattc ttctggcttt
    31741 cgcagcattt gcgtgctttt atattgggga agaatttcaa aacggtacga ttcgcaatac
    31801 gttatcttta ggacggaatc gaatgactta ttatgtgtcg aaacttctcg ttacgctttt
    31861 gatcacaaca cttggcgtgg ttttgatcac gggactagcc atgcttgctt acacacttgc
    31921 tttcggattt ggtgaagttg agggaatcaa aaactatgga aattatgttc taaaagtatt
    31981 tcctgtattg cttctgctga cattagggac attatctgtt ccagtggctt tgacattcat
    32041 cactagaagt accagtgtca gtttgcttct ttcttttctt tatatcatgg gaacggcctt
    32101 cattcccggt gttttcgcaa aaatcaaagg cttggaattt ctaacggagt ggtttacgga
    32161 aacgtggctc atgtatacgg actttgcgca acaagcaact tattcgcaag caccgaaaat
    32221 gattctggtc agtctagtaa caatcgtggt atcatcagct ctaggaatgt ttattttcca
    32281 aaaatctgac attaagtagg tggtcctgct ggaaactatt ttgattatcg aagacgatga
    32341 agctgttcat tccttactgg aggaggttct tgaacagaga tacaaacttc ttgatgctta
    32401 ttcaggtacg gaaggaaaat tattgttaag cacatatcct gtcgatttga tccttctaga
    32461 tctcatgctt ccagggcttt caggtgaagc attgttagca gaaatccgac agacatcgaa
    32521 tgttccgatc atcgttcttt cagcaaaaag cgatcaaaga gataaagttt ccttgttggc
    32581 agcaggagcg gatgactatg tgacgaaacc atttgatatc gaagaacttt tgttacgaat
    32641 cggcatccaa cttcgtcatc agacttctgt ccaagtaaaa gatctgtcgc aacgcatcta
    32701 ttacaaggaa atcttactgg acaaagagtc gagggatgta aatgtgaatg gtgtggctgt
    32761 ccatttgaca ggaagagaat tcgatctgct gaagcttttt ttagaacatc cgaaaaaagt
    32821 attttcgcga gcaaatcttt atgaaacagt ttggcaagaa ccatattttg atagtgagaa
    32881 aacagtgaat atgcatattt ccaatcttcg agggaagcta gcaaaggcac atgctgtgta
    32941 tattcatacg gtgtggggag tggggtttag atttgattaa atggctgttg cttctcgttg
    33001 ttatctttat gggaagtttt attttcttcc tgcaaaggga aatcaagaga ttaactaaga
    33061 aaatcgccac tctttctgaa cgacaagaat atggtggtcg tctttcagtc gaatttcgag
    33121 aaaagaatct aatggaccta gtagatgcac tgaatacatg gttggatgaa aatgaacaaa
    33181 aaggacaagc atttcaagaa atggaagaaa acatcagact atcgatcgtc ggcctttctc
    33241 atgatctcag aactcctttg actgctatta acggttatgt acaattactg gaacaggtag
    33301 aggatacgga gaaacgggag aagtatttaa cgatcatcaa acaatctgtt gcaagattac
    33361 tcagtatgac ggatcagttt tatgatcttg ctcgaataga gacgaagcaa aaagaaatgg
    33421 atctccatac gctttctttt tctaaagagg tggaagaagt atttttctct ttttatgaac
    33481 aattttctga gaaaaatatc gaggtagcgt tcgctgaaca ctcgataaat acaactgtat
    33541 tagcagatcc gctcatgttg ctgcgagtgc tgcaaaatat catacagaat ttattgcgtt
    33601 atgcagatgg acaagcaaag gtgacatttt caaaagagga tccattcatt cgattaacag
    33661 tggccaatcc tatcaaacca ggaacgcaaa cctctgttga aaaagtcttc cagcgttttt
    33721 ataccgaaaa taccagccgc acaaatacgg aatctagcgg aatcgggctt tacctgtcaa
    33781 aaaaattggt ggaaggaatg ggaggagaga tgaccgctag attagacggg ggcatcttct
    33841 ccatctccgt caagttgcgg agggtttaat tatacaagat tagtgagaac tagtagaaaa
    33901 atgtattatc ttatatatat ttaatagaga aaaaagcctg tgatataaat attgcaggct
    33961 tttttattga tttgaaataa tcaagaatat tagaaacttc tacattaatc aagaactttc
    34021 ttctgtatga aaatagagat aataattgta cacttaagct gtattcagga ggtgaagata
    34081 atgggcatcg aatttagcga acggcaaatg aaaatactat actttttgat tgaaaatgag
    34141 cacacaactt acaaagaatt atcagaacaa ttatatgtat ctacgaaaac aattagcaaa
    34201 gatattttaa ttttgaaaga aataatccaa aaaatgccag atgtacaaat gttggttcaa
    34261 cctaaaaaag gcgtttcgct tattggaaaa aaagaaacac ttcgaaattt attatccagt
    34321 cataaaatca atatcgcaga ctctccggag gaccgtctaa cttatgtgtt tgctaaatta
    34381 ttgaatacta atgattctct aaagattcaa aatcttgctg acgaattata cgtgagcagg
    34441 tcgacggtag aaagcacaat gaaagatgtt agaaaaagac taaacaagca tgggatgtcc
    34501 ttagtttcag atcgaaatgg cgtgcgcatc aaagctgatg aaaaaagtaa aagaaggttg
    34561 atgtcagaag ttgtgaacta ttattggggt ggctttacag catctaacag taaaacagat
    34621 ctcttagact tgaatatcaa tttcattggt gaagcgaata atttcttaaa taaggacata
    34681 ttgaaaaaag tagccgaatt gcttaatcga tacatgaata agacacaatt gaaatttaca
    34741 gattatgaat ttcaaacttt agctatccac cttgctattg cgatagaaag gattgaaaca
    34801 agcaatttct tatcacgtag taataattct gaagaagaaa tctcaaagaa tacacttatt
    34861 ttaatcaaaa tgattgaaaa agcattttca attattgtgc caatagatga acaagcttat
    34921 gttgacattc acattacttc gattgaaaaa aatgctatga atcacgactt agatcaaacg
    34981 aagctttcct atttagatat gggtgttgag ctacaagagt tagtaagaat taaattaaat
    35041 gaattttatc ctgatgaaga attgatcaat aatctagtat tacatctcaa tgcagcagtt
    35101 aaaagactca aattgggagt aaatatctat aacccttata cggacaaaat caaatattct
    35161 tttaaaaggt catttatgat aagtgtagat ttattagagg aaatcgaaga gagattctgt
    35221 atacatttta atgaagatga aatcgcttac gtaacactcc atattcaatc acttttggat
    35281 cgttacaaac ctgacaaaac gaaagtgata ttagtttgta gtagcggata tggaacatca
    35341 aaattattag agcaaagaat aaataacggt tttgcagata tggttgagat agaagatgta
    35401 ctaagcatca atgaactaca agatagaaaa gtcactgatg aacttgttat aagcacgatt
    35461 ccgattgaaa atacaaattt tcctgttatt gttgttagtc ctttgatgtt aggggcagat
    35521 gtcagaaaag taaaacagta tctacagtta aatgaacaaa cagaagatgc ttttctaaat
    35581 ttattagata aacgattttg cttttttacc caccaaataa aggataatca agtgaacgtt
    35641 ttaaatataa ttaccaaaaa attggtaaaa gaaaattacg cgaaagaagg gatactagag
    35701 ggagcaatca aaagagaaaa actatcttca acagcgatga atgattttgc tatgccgcat
    35761 atcgaagtag attttataaa aaagccagta atctgtgtct acgttaatcc agaaggaatt
    35821 gattgggatg ggacacttgt tcattctgtt ttcttttttg cacttaatga aaaagtaaaa
    35881 cctgaattga ataaaattta tgaaacattc aatgaaatcg cttcgaatca tgagttatta
    35941 gcaaatttaa aaacgagttc gtcgtttgaa aaattagttt atatattgaa agaaggctta
    36001 taaatgatca gtgaaaaaat caatataaat aatatagtga tagatttaaa tgcatctgat
    36061 aaaaaaagtg tagtcgaaaa attatcgaaa ttgttagaga ctaatgggtt tgtgtctgac
    36121 cacaaacaat ttcaaaaaga cgtatttgac agagaaaacc atatgaccac aggaattggg
    36181 aaaaatatcg ccattcctca tggcaaaagc ctagcagtaa ttgaatcaac agtggctgta
    36241 gccaagttaa agcggtctgt tgaatggggc gcattagatg atcaaccagt tgatctcgtc
    36301 tttttgttag caataaaaga cactgataaa ggtactaatc atttacgtat tttagcagaa
    36361 ttatctggaa gattgatgga tgacgagttt gttaaaagaa taaaagattc gagaaataaa
    36421 aaagaattgg taaaagctct atcattttaa aaggaggaat ttcatatgac aaaaatagta
    36481 gcagtaacag catgtatcgc agggattgcc catacttata tggcaaaagc taatttagaa
    36541 aagtttgcga agaagaaagg gttagatatt aaagtagaaa cacagggggc tatggggata
    36601 gaaaacaaac tttcaatttc ggatgtttcc gaggcagatg ttgtcatatt tgcagtagat
    36661 acaaatgttt cagaacgagg tcgctttgac ggtaaaccga ttttaacagt agggacctca
    36721 gaggtagtaa aagatggtga agagacgatt gatgaagcaa ttgcattaat taacaaataa
    36781 aaaatatatc gtgggggagt tactaatgga attcaagaaa caaggaagag aaattctaaa
    36841 tcatttccaa agtggggttt cctatatgat cccgcttgtc gtagcagcag ggttactaac
    36901 ttctattgca gtcatttttg gtggcacagg tgtttgggac cagacggata cattctgggg
    36961 agtcctgcga atgattggtc agacgggatt acagtttatc gtaccgatga tttcagccta
    37021 tatcgcatat tcaattgctg accgtcccgg tctagcacca gcttttatca ctgggatgat
    37081 gtgtcaaaat cttggtatgg gatttattgg aggaatggtt gctggattag cttgtggcta
    37141 tttagctaat gcattgaaaa aagtaaaagt accgatgcaa gttattagtc taaaatcaat
    37201 tatgctgatt ccttttgtta ctacattagt tgttggaatc atattatggt atgtactagc
    37261 tacgcctatt caagcaatga caacaggact gactaattgg ctagaaggca tgtcaggagc
    37321 aaatgctgct ttgatgtcgg ctattttagg aggaatgatg gcgtttgata tgggaggacc
    37381 tatcaataaa atcgcatatg cttttggggt tgcttctttt tcaagcggaa gctttggacc
    37441 tagtacagcc atgttattag gaattgctat tccgccattt ggaatgtttt tagcaacaat
    37501 tttaaacaaa aaactttatg atgaatcgga acgagataat gcaaagacag ctatgattat
    37561 gggccttgtg gggattactg aagggacgat cccgtttgcc gtgaaagatc ctttacgagt
    37621 aataccaagc atcgtggtgg gcactgcagt agcatgtggt ctaaatggat tttttggtgt
    37681 tactcaagaa acaatgcttt caacctttat ggcaattcct tttgttacga atataccttt
    37741 atatttagta tcaattgtta ttgggggagt ggttacagcg ttaatggtca atgcactaaa
    37801 atccatcaag tataaaaaag aacaacaagt aaattcagta gaagaaggaa agtaggagaa
    37861 tctgatatgg taaatgcaca tataattcaa cacacgcact gggacagaga atggtatttt
    37921 acaaatgaag atgccatcgt attgtctgat caggtcttta ctgaagttct agatgaattg
    37981 gaaaggaacc ctaaagtaaa cttttgttta gatggacaaa cttcaatcgt cgatgagtat
    38041 ttggagatca atccgcaaaa gcttaaggtt atccaacgac tgatgaaaac aggacaacta
    38101 ttcgtgggac cgtggtatgc acaaacagat gccttattag tggatgctga atcaatttta
    38161 agaaacttaa tgattggaat cagtgatacg atccttaaat atggagatcc aatgatggta
    38221 gggtatttgc ctgacacgtt tgggttcaat gcgaatttgc ctacactttt gcatcaagta
    38281 gaaatcgata atttcatgtg ttggcgagga ttaaactttg aaacgatggt tccatctcct
    38341 tattttatct ggaaaggatt gggagataaa tttgtttatg ccatgaattt tccatttggt
    38401 tatatgactg gaatgatgac cttagacgca attaaagata ttcctgaatt tgcaaaagaa
    38461 aagttggatg aagcagttga aatgcttcat atgcaaggaa aaaacaaaga tattttagta
    38521 ccttcgggtg tagatcaaaa gtcaatgatt ttaaattttg atcaagtcgt agagaagttg
    38581 aataaataca gtaaattcca caatattatt tcagattatc cttcttatgt agaagttatc
    38641 agaaacaaaa aagaattacc tgaatataaa ggtgaacttc gtcagccggt ttactctcgt
    38701 gttcatcgtt ctattggctc agtaagaaca aaaatgaaaa tagaaaattt taaattggaa
    38761 caaaaaattc taaaacgagt tgagccgctc atgctgattg cagaaagaaa tggtatccat
    38821 atcagtaaag gcctacttcg tcggttatgg aaaaagattt tgcaaaacca ggctcatgat
    38881 agtattggtg gctgtgtttc agataatgtt gcggaagata ttttccatcg aatcaaagaa
    38941 gctaatgaga tagctgacgg aatcgaaaac cttattgtaa aacggatggc tgatgcatta
    39001 aagctaaaat cgaatgaagt ccttgttttc aacacaacga ctgagaatta tagtggatcg
    39061 aaaatcgtgc atgtagtagc tgattcaaag aaaatacact tcaaaggtag tccctataat
    39121 atcattgaaa aagaagttta ttttccagaa cggaaaaatg cttatcggat gattgctaca
    39181 ggtttcgaat attttgatga accagcttat tatgaacttg acataaaaat aaatgtaaat
    39241 gtgccagcac taggatacac tgttgttgaa tttgagaaaa ataatgaaaa attagaaaca
    39301 gcaatcgaga tagacaaaac tgagataagt aacaataaat ataaactttc ttttaaagat
    39361 gggcaattga atttgatagt tggagataga caatatcttg atttcgttca tttgatcgat
    39421 tcagcaaatg atggcgatac gtatgattat tcaccattag aaggagatac agaactatct
    39481 ctaaaattag aaacagctaa agtatataaa gattctttac aagaaacatt agttgtttac
    39541 ggaaaagctc aacttcctaa agatttaaaa gatcgtttat ctgaaaaacc cgaaatggaa
    39601 gaaatatctt atgaaatatc gttcagtctg ggagaaagtc aaatcgttga aggcacactc
    39661 aaaattcata atactgttta ctcgcatcgt atgcgcttag caattaatgt tttagataaa
    39721 tctgaaaaat caattgcaca aattcaaaac ggatttattg aaaatacccc tgatgtcatt
    39781 cctccgaatt ggtcagaaac tatggtagag aaacctgtta atttagaaat attcgataag
    39841 agtgtctctg tggcagaaaa tgatcattat tttacgtttt atgcagatgg tttaaaagag
    39901 tatgaacgta tagaaaatca attaatcata accttatttt caacaacagg ggaacttgga
    39961 aaacctaatc ttgcttggcg acctgggcga gcttcaggag atacgacaaa tgaagggcat
    40021 gtcatgatgg aaacaccgct agctcaagag ataggtgagt ataaagtcac ttttggattt
    40081 aatgtagaag aaggaagatt taatgagttc aaagtggcaa aacaggcaga aaaacggtta
    40141 gaacaatcta tttcttatca aaagcagaaa ctgaatgtat ttattcacag gttagataac
    40201 aagatttggc cattacaaaa tgaacttgat gttcctcgta aattttcatt gattgaatta
    40261 cctgaagact taattgtatc atcgatttat ccttcttatt ttcatgaaca aggatttgtt
    40321 cttcgcttag ctaatccaac agaacaagaa aaaatagtac ctgaggctat cctttccctt
    40381 gggacagttg tcaatgcact ggagaataaa caagaattaa caacaattcc tccctatgat
    40441 tatttgtcaa ttttgataaa tgagagataa atatgtaata tacaagaaaa aaacaccttt
    40501 tattttgaaa ggtgtttttt taccctcttt tcaaatagcg caacagtgct atactaaagc
    40561 taacaaataa aggaacggag agggatatgg gtcaaagaga atttatcgtt ttaattatta
    40621 ttgcagctgt tattttatta gttgctttag acattttttc acgtttgaaa ttaaaggaaa
    40681 cagttcgtag caaatgggaa aaaattcctt atcaaccgag atttgataaa gaagaaagct
    40741 tgaaggaagc atggctcacg gagaagaagt tccgttcatg ggatagcgaa atcgatgatc
    40801 ttacttggta tgacctggat atgttcgaag tattcgaagg aatcaacagt acctattcta
    40861 gtgtaggttc agaagcgttg tatcaacgtt tacgcagctt tgattttggt gaagatcagc
    40921 agttagaaaa actgattgct ttttatcagg aaaatcctca actgagagaa aaaatccaat
    40981 atcaattcgc tcgtttagga aaaaaagatc ataattttgc aaaacaatat ctagcggatg
    41041 gaaaaagtaa aaagattgga cgcgtaggtt tatttatttt tcttggcttg cttccgctgg
    41101 tgggtttggg tttgcttctt ttaggacaag tactaggaat ctatttggca atcggtgggt
    41161 tagtattcaa tacggtctat tatctagtga agaaacaaac actagaaaca gagctgaata
    41221 gcatgagtta tttggtttca acgatttcag ccgccaatca gctagcaaaa ctgccgacac
    41281 cacttcaaga agagctgaaa cagcaaacta aaccattaag agacatccct aaattaggtt
    41341 tttcatttcg tatgaagagt gggagtgaag cagagttgat ttttgaatat gtcaacatca
    41401 tgttcatgct gccatttatc tcctatcatt tcgttctcac taaattagaa aagcattcaa
    41461 ctgaagccat caagctgtgg gaattgctgg gagaattaga agtagccgct gcaatattga
    41521 attaccgtac ttatatgccc atcacttgcc gaccagagtt tactgagggc ggtgtgacag
    41581 cgaaagatat ctatcatcca ttgttaaaag aagcggtagt gaatccggtc aactggcaga
    41641 agaatacatt agtcacgggt tccaatgcat caggaaaatc tacgtatgtc aaaagtatag
    41701 ccatcagctg tattctagca cagacgatcc aaacagcgat tgcagaacag tttaccctgc
    41761 aacctggaca tgtgttgact tccatggcaa tcgaagatga tttatttgaa ggcgacagtt
    41821 attttgtttc agaaatcaaa tcgatcaaac gtttgttgga ccaagtggct tccaaagaac
    41881 gctgctactg cttcgtagat gagattttaa aagggacaaa tacgatcgaa cggattgctt
    41941 cttccgcttc agtcgttcgg tggcttgcgg agtatccatc tttagcattt gtcgccacac
    42001 atgatatcga attgacagag attttaaaga attattgtga gaatgtccac tttgaagaac
    42061 aagtaacaga aggaaaagga atcagttttg atttcaaatt aaaagaagga ccttcaagga
    42121 cccgaaatgc gattgccttg ctgaaagttt tggattaccc agagacttta gtagaaaccg
    42181 caaaaacgga agccgcgtta tttgatgaaa agcggcagtg gcatgtattg ggataggcga
    42241 agtgatcgaa agtatttatt ggatcaaaaa tcttggcttt tgcttccgcc gtttatacgt
    42301 gtttagagcg tgagacaaaa gtgttcttta cttttgtctc acgctccttt tgttgcacat
    42361 atgaatagta atcctttatc ttcgatttgt tttttctgaa aaggttgtct gacttcgtcc
    42421 gattgatgtc tttcattagg agattttcct gagcccagca acctgatttt tcgctggaac
    42481 caagttgttt tttcatctgc tgtattccat gatacaataa agaatcatgt ctataaggag
    42541 ataggaaata aatggcaaat gcgcttgaaa taaataattt gaaaaaggtt tatggaagtg
    42601 gggtcgaagc attgcgagga atcgatcttt ccgtgaaaga aggagatttc tatgcgctat
    42661 taggaccaaa tggagctggg aagtctacaa cgatcggaat cattacttct ttagtcaata
    42721 aaacatctgg aaaagtgaat gtcttcggtt atgatctgga tacggatcta gtccgagcaa
    42781 agcaacagat tggattagtc ccgcaagagt tcaatttcaa tccctttgaa acggtccagc
    42841 aaatcgtcgt gaatcaagca ggctactacg gtgtctctcg caaagaagcg atgaaacgca
    42901 gcgaaaaata tttgaagcag tccaatcttt gggaaaagag aaatgtccgt gcacgtatgc
    42961 tttccggggg aatgaagcga agactgatga ttgctagggc attgatgcat gaaccacgtt
    43021 tgctgatctt ggatgaacca acagcaggag tcgatattga attgagacgc gagatgtggg
    43081 agtttctccg agaattgaac gaaaatggta caacgatcat tttgacgacg cattatttag
    43141 aagaagctga aatgctttgt cgcaacatcg ggatcatcca atcaggtgaa ctgatcgaga
    43201 atactagtat gaaagagctt ctatccaaac tgcaatttga aacatttatc tttgatttag
    43261 cgccatatga gaccaaacct gtgattgaag gatacgcttg tacatttgaa gatgaacaga
    43321 caatcgctgt cgaagttgaa cgaaatcaag gggtgaacgg aatcttcgat cagctgacaa
    43381 gacaaggaat caaagttctt tctatgagga ataagtcgaa tcgtttagaa gaactgtttt
    43441 tgaaaatcac agaagaaaat aatcatacgg aggagaaaaa tgtttaatct ttattttact
    43501 gcattaaaaa gtttagctat caaggaaacg aaccgctatt tgcgtatctg ggtccaaaca
    43561 ctggttccgc ctgtgatcac gacatccttg tactttgtta ttttcggaaa aatgatcggt
    43621 ggtcggatcg gtgacatggg tggtttttca tatatggaat tcatcgtgcc tggtttgatt
    43681 atgatgtctg ctatcactag ttcgtatgcc aacgtttctt cgtccttctt ctctcaaaaa
    43741 ttccagaaaa acatcgaaga aatcttggta gcgcctgttc ctacgcatgt gatcatttgg
    43801 ggctttgtga ttggcggagt gggtcgcagt attctcgtcg gctctctcgt aacgatcatt
    43861 tccttgttct ttgtaccact tcatgtgtat tcatggttca tggtcatcat tacattattg
    43921 atgacggcaa tcttgttttc gttagcagga ctgctcaacg ggatttttgc tcagtcttat
    43981 gacgatgtat cgatcgtgcc tacatttgtc ttgcagccgt taacttatct aggtggcgtt
    44041 ttctatgcca tctctatgtt gccgccattt tggcaagctg tttcgaaagt caatccgatc
    44101 gtttatatga tctccggttt ccgttacgga ttccttggaa caatcgatgt gccagtagca
    44161 acatcgatga tcgtacttgt gctattcatc gttgttttgt acgccgttac ttggtatttg
    44221 atcaataaag gaagaggatt gagaagttag aaaagaggtt gtgaaatcag cgttttgacc
    44281 tgagtattag agaaacaaac gggaaaatag ttcctcatat tttgtcgttt gttgagctaa
    44341 taccgagggt cagctgattg aacaccgttt agtggagagg ttgtgaaaaa gctgttctgc
    44401 atcaagtaat aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttggact
    44461 tattaccgca gatgcaagct tttgaacacc atttattccg gaaaaggaat tgtgataaga
    44521 tttttgtcac agtctttttt tgctcaatca gaaacatctt ttaagctgtt cactttgctt
    44581 ctttttttgt ttcctagtat aatgacaatg tttttgagaa agattgcgac ttcactgctt
    44641 gatagaatgg agagtaacaa ataaatgact actaattttt ggaaagagct gccaaagcct
    44701 ttttttgttt tagcaccaat ggaagatgta acagatgttg tatttcgcca tgtcgtaaag
    44761 catgcagcag caccggatgt cttctttact gagtttacaa actcagacag tttctgtcat
    44821 ccagatggaa aagacagtgt acgaggaaga ttgaccttta cagaagatga acagccgatg
    44881 gttgcccata tttggggaga caagccagaa ttttttagag aaatgagcat tgctatggca
    44941 gaaatgggct tccaaggaat cgatatcaac atgggctgcc cagtccctaa tgtagcagaa
    45001 cgtggaaaag gcagtggctt gatccttcgc ccagaagtag cggcacaatt gattgaagcg
    45061 gctaaagctg gcggtttgcc tgtcagcgtc aagacgcgta tcggctatac ggaaatgtct
    45121 gagatggaag aatggatcag tcacctactt catcaagata tagccaatct ttctgtccat
    45181 ttgcgtacgc gtaaagagat gagtaaagta gatgcacatt gggaagtcat ccctaaaatc
    45241 attgctttac gtgaccaaat cgctccgcaa acactgatca cgatcaacgg agatatccca
    45301 gatcgccaga caggagaaga attggccgaa aaatatggcg tagatggcat catgattggc
    45361 cgggggattt tcaagaatcc ttatgcattt gaaaaagaac cgagggaaca tacagaaaaa
    45421 gaactgatcg gactacttcg tctgcaacta gatctgcaag atcattattc agaaatcatc
    45481 ccgcgttcca tcacaggact acatcgtttc tttaaaatct atatcaaagg atttccagga
    45541 gcaaatgact tgcgagttca gttgatgaat acgaaatcta ctgatgaagt acgagagatc
    45601 ttagacacct ttgaaaaaga acatccaacg cttttcagtg aacaatgatt tcttgcttga
    45661 acaccgtgaa ttagaggtcg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    45721 caaaaatagc tcctcatatt ttgtcgtttt ttgagcgaat accgagggtc agttgattga
    45781 acaccgttta aaagaggttg tgacaaaagt cttgtcgcaa cctctttttt aaccaaaaaa
    45841 gtgcaaatag tttactttaa gtaagagaat aattatgatg aataaaagtg gaacaaatag
    45901 catactatat atattgaaat gaaaactgta tggatgctcc aacttgatga agctaaggag
    45961 gaattcgtat gtcagaaaag gaattaatga aggtagtcgg tttttatgaa gggttgtcga
    46021 tcgatgatcc aaaaagcttt attgatgaaa aagaacagat acctgtaccc agttcacggg
    46081 atcttctggt aaaagtcaac gcagtatctg ttaatcctgt cgatacgaaa cttcgccaag
    46141 ataacgggat aagaaatgct ttacgtatct tagggtttga tggagtcggg aaagttgttg
    46201 ctgtaggtga tgaagtgcaa aaattttctg tcggcgatcg cgttttttac gcaggtacaa
    46261 cgacaagagc aggaagcaat caagaatacc aactggttga cgaacgtatt gtcgctcttg
    46321 caccaaaaaa tctttcagat gaagaagccg cagccttgcc gttgacctca ttgaccgctt
    46381 atgaattgtt atttgaaaaa tttggtctga ctccagaaga aaatgccaac cgcgggaaaa
    46441 aaatcttagt gatcaacgga gctgggggcg tcggttcgat tttgaatcaa ctagcgcatt
    46501 gggctggcct agaagtctac gcgactgcaa gtccgaagaa ttttgaatgg ctgaaaaaaa
    46561 caggtgtaga ctatccaatc gattatcatc acgacttaag taaaagtttt cgtgaattag
    46621 gaatcgatca agtagagtat gtcgctgtat tattcgatat tacccgctac tttgatcaaa
    46681 tcaaacacct tgtccgacca tttggacatg tgggaacgat cgtagggatc aaagagccgt
    46741 tagatatagg tgtttggaaa aaccaatcta tcagcttcga ctgggagtat atgttcgcta
    46801 aaacagacta taactatgaa atcgaaacgc aaggagaagc tttagcgcac attgctgctt
    46861 tagcaaatga agggattatc cgcagtacat tgagcaaggt ctattcagat gggatgaatg
    46921 cgaagaactt gaaacaagca accaaagacg tagaaacagg acatatatta ggaaaagtcg
    46981 ttgttagtgg tccgttcaac ggataagcaa gaatacgccg atttttttct tatttgactt
    47041 tgataaaaac aagagcgcat gacagtaatt tgccataagc tcttgttttt ttatttataa
    47101 taagcgaatc aacgaaaggg aaagaattcc taccaagacg atcaccaata agctttttga
    47161 aagaatagca gctaaaagtg taggaactga agccaaagca ttttcgagat tgatctgcgg
    47221 cagatgtccg atattttggg taaatagact gctgaaccat aaagcagaca taatgacgat
    47281 cgggacaaaa cttaaatact caagcaacgg ttgcggcaaa tcgaattttt tcagtaagac
    47341 aaatggtagg atcctagaaa gccaagttgc taaagtacaa cctaaaatag tcagcaatgt
    47401 aaactcaaaa gaaggcatgt ttgatccaca cccccaatcc gcacccaatc agtgttacaa
    47461 ctaaaatcac taagttactt gggataaaaa tcagtccgac atataccaga atcaaagtga
    47521 gaccaatcag gatcaactgt aatcgtttag aggtgtttct atcagaaatg acttgaagat
    47581 aaagtaaccc gataaacata gcgacgatgg cgaaatccaa accaaacttt ttcggatcag
    47641 taataaaatt ccctaaaagt gcgccaacaa gagaagaacc cacccacgtc aaataagaaa
    47701 tccaattagc tgtattcatc caacgaaagt ttaacttttg tccagtatag ttcaatttat
    47761 tcattcccag tgcgaaactt tcatctgtca gcaatgtgcc aatcagcaga ttttgaagca
    47821 gccgattttt cttgaaataa ggagcaatcg tcatactcat cagaatcatt cgggaattga
    47881 ctagaaaggt cgagagaacg atggagagca gtggactatg gctagcaagc atgctgaccg
    47941 tgataaattg ggcagaacca gcatagatca gcagggacat caacgtgacg accagcggat
    48001 gaaacccggc agctttccca acgataccaa atgcaagtcc aataccgata taaccaaaaa
    48061 cggtaggtaa cgtgtctttg atcgcagttt ttatatctaa cttttcatcc aataacaaaa
    48121 ctcctttagc acgacataaa ttttcataat tgtcgtcaaa cttgtgaata ttctaaattt
    48181 ttaatgaaga cttgtcaact tcaattttaa acaaataaaa aagactggac gtagtttaaa
    48241 aaaatcttct caagttagat ttccagacct aacttctgag gatcactaca tttgtcacag
    48301 tctcttatac tgacaagtat aaatgaagta tgaagaaaaa gtatttgtta ttggtatttc
    48361 tcattattct tgttttctaa tgtgttcact tcaagtggaa ttttagaata ttattcaact
    48421 aacaagtcta catcgtatct aaatccattt gtatcactta attgcttgta aaattcgcca
    48481 ctttttttaa ttgttctttt ttgagtagta aggtcgagtg aaaccagccc atatcggttt
    48541 ttataggcgt tgatccatga ccagcaatca ataaatgtcc aaagatgata ccccttaatg
    48601 ttacatcctt catttattgc cttccataag taagcaagat gatctttgat atactcaatc
    48661 cgataatcat caattatttg ccctgtatca tccatgaaac gttcttcatc cgcaacgccc
    48721 attccgttct ccgagataaa tgcttcgatg tttccataat ggttcttgat atccatgcac
    48781 aaatcataaa ttcctttttc gtaaatttcc catccccggt attcgttcat tcgtcgtcca
    48841 ggcatgatat gtggttcgaa aaaccactct ggtaaaaatg gactagatgt atccaccata
    48901 gaagaacgag atttgacgcg acgtggctcg taatagttta gtccaagaat ttgagcggta
    48961 ttttcgtaga tacatacaag atcttctttc gtataatctt caggaagctg attatattct
    49021 tgtaataaca ggataagctt tttagaaaaa ttccctttaa ccataggatc taaaaagctc
    49081 cgcgtataaa ataaatcagc gatttctgct gctttcacat cagcaggatt ttgactacga
    49141 ggaaaagtag gactcaaatt tagaataacg cctattttac taggaagagc tagttcttta
    49201 aatacttgta ttgcttttgc gtgtgcaagt atagtgaaga atgctgcttg cgctccgcgt
    49261 ttaaagtcaa tgaggtttgg ataatgaaaa tcttcaagat aagtacctaa aataggccca
    49321 agtggttcat tgaaggtaaa ccaatgacaa actaagtcac caaattcttt aaaacaagtt
    49381 tcagcgtaat gaacataagc atcaactact tcacgtgatt cccatcctcc tttatcttga
    49441 agagtagcag gcatgtcaaa atgatataag ttggcaaata ctttgattcc gttattattc
    49501 atttcggaaa ataattttcg atagaaagaa atggctttgg gatttacttt tccggtaccg
    49561 ccatttggaa acattcgtgc ccaagaaata gaaacgcgaa aggagttatg cccagttttt
    49621 ttcattagtt ctatatcttc tttatattga tggatgtgat cggttgtaat agaggaatcg
    49681 attcgttgat aaaaccggta aggttgttct ttaaaccagc gatcccagat agtctctgct
    49741 tttcctgttt ctccttttcc ttctgcttgc tcagctgacc aagcggaacc ccaccaaaaa
    49801 tcatctggaa aagttagctt tttcatagtt gtcataattt ttttacctcc tgtttccact
    49861 taatgataac accgcttaca acttaaagta aatagttttg tttaaatata tatatctttt
    49921 ttattgtgtg tataattttg atgtggattt tatcggttta tttatgttaa agtatagatg
    49981 aagatatgtt tttgattgga gggtcaccat tgaaaaaaat tgcgaagtat atggaaattt
    50041 acttagatat aaagaaaaaa attgtaaatg gagagtataa aattggtgag aaacttccta
    50101 caggagatga attggcagtt atttatcata caagcaagtt aacagtaaag aaaggcgtag
    50161 acttacttgt ttccgaagga gttttgcgaa gtagaagtgg gtatgggact gaagtcgtca
    50221 gaacaccaat tgataactca cgagttcttg gtccaaacga aggtttgttt agcgtggttg
    50281 gtgaagaaca cgtagattca gaaatccata ctttctcaat tgaacttcca tcgaaaaaag
    50341 tagctgataa attaagtatc agtgtaaaag actatgtata taatattgta cgaagccgtt
    50401 ttattgattg tcaaccatac tcgattgagc aaacattcat gccattagca gtcataccag
    50461 gcttggaacc aaaacattta aagaaatctg tatatgctta tatcacaaat gaactgggtc
    50521 tggaaataaa gacttcgcac atacgtatca aaggagatac tgcgaatgaa tttgacgcaa
    50581 aaatattaaa aatagatcca ggtgaattta tgattgagat agacaaagtt gttgctctat
    50641 cgtctggtaa acctttcgag tattcggtat cgagacattt atatcaagac tttgtttttg
    50701 aagctgtttt tgttgaaaaa taagc
//
LOCUS       DAZAJZ010000008        47554 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000008 DAZAJZ010000000
VERSION     DAZAJZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.008"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..928)
                     /locus_tag="ACG86D_000496"
     CDS             complement(80..928)
                     /locus_tag="ACG86D_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3139671.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1152..3092)
                     /gene="thrS"
                     /locus_tag="ACG86D_000497"
     CDS             complement(1152..3092)
                     /gene="thrS"
                     /locus_tag="ACG86D_000497"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN3139672.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3429..3608)
                     /locus_tag="ACG86D_000498"
     CDS             complement(3429..3608)
                     /locus_tag="ACG86D_000498"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN3139673.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3655..5478)
                     /locus_tag="ACG86D_000499"
     CDS             complement(3655..5478)
                     /locus_tag="ACG86D_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3139674.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5756..8035
                     /gene="helD"
                     /locus_tag="ACG86D_000500"
     CDS             5756..8035
                     /gene="helD"
                     /locus_tag="ACG86D_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN3139675.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8128..8775)
                     /locus_tag="ACG86D_000501"
     CDS             complement(8128..8775)
                     /locus_tag="ACG86D_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139676.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8954..9673)
                     /locus_tag="ACG86D_000502"
     CDS             complement(8954..9673)
                     /locus_tag="ACG86D_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3139677.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9804..10955)
                     /locus_tag="ACG86D_000503"
     CDS             complement(9804..10955)
                     /locus_tag="ACG86D_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN3139678.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11172..11912)
                     /locus_tag="ACG86D_000504"
     CDS             complement(11172..11912)
                     /locus_tag="ACG86D_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3139679.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11916..12239)
                     /gene="rsfS"
                     /locus_tag="ACG86D_000505"
     CDS             complement(11916..12239)
                     /gene="rsfS"
                     /locus_tag="ACG86D_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN3139680.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12296..12889)
                     /gene="yqeK"
                     /locus_tag="ACG86D_000506"
     CDS             complement(12296..12889)
                     /gene="yqeK"
                     /locus_tag="ACG86D_000506"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN3139681.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12873..13523)
                     /locus_tag="ACG86D_000507"
     CDS             complement(12873..13523)
                     /locus_tag="ACG86D_000507"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN3139682.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13533..13844)
                     /gene="yhbY"
                     /locus_tag="ACG86D_000508"
     CDS             complement(13533..13844)
                     /gene="yhbY"
                     /locus_tag="ACG86D_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN3139683.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13860..14969)
                     /gene="yqeH"
                     /locus_tag="ACG86D_000509"
     CDS             complement(13860..14969)
                     /gene="yqeH"
                     /locus_tag="ACG86D_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN3139684.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14966..15493)
                     /locus_tag="ACG86D_000510"
     CDS             complement(14966..15493)
                     /locus_tag="ACG86D_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN3139685.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15673..16458)
                     /locus_tag="ACG86D_000511"
     CDS             complement(15673..16458)
                     /locus_tag="ACG86D_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN3139686.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16451..17320)
                     /gene="accD"
                     /locus_tag="ACG86D_000512"
     CDS             complement(16451..17320)
                     /gene="accD"
                     /locus_tag="ACG86D_000512"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN3139687.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17321..18700)
                     /locus_tag="ACG86D_000513"
     CDS             complement(17321..18700)
                     /locus_tag="ACG86D_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN3139688.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18708..19127)
                     /gene="fabZ"
                     /locus_tag="ACG86D_000514"
     CDS             complement(18708..19127)
                     /gene="fabZ"
                     /locus_tag="ACG86D_000514"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN3139689.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19152..19628)
                     /gene="accB"
                     /locus_tag="ACG86D_000515"
     CDS             complement(19152..19628)
                     /gene="accB"
                     /locus_tag="ACG86D_000515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN3139690.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19631..20866)
                     /gene="fabF"
                     /locus_tag="ACG86D_000516"
     CDS             complement(19631..20866)
                     /gene="fabF"
                     /locus_tag="ACG86D_000516"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN3139691.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20885..21622)
                     /gene="fabG"
                     /locus_tag="ACG86D_000517"
     CDS             complement(20885..21622)
                     /gene="fabG"
                     /locus_tag="ACG86D_000517"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN3139692.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21634..22551)
                     /gene="fabD"
                     /locus_tag="ACG86D_000518"
     CDS             complement(21634..22551)
                     /gene="fabD"
                     /locus_tag="ACG86D_000518"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN3139693.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22658..22882)
                     /locus_tag="ACG86D_000519"
     CDS             complement(22658..22882)
                     /locus_tag="ACG86D_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN3139694.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22935..23897)
                     /locus_tag="ACG86D_000520"
     CDS             complement(22935..23897)
                     /locus_tag="ACG86D_000520"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN3139695.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23907..24347)
                     /locus_tag="ACG86D_000521"
     CDS             complement(23907..24347)
                     /locus_tag="ACG86D_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3139696.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24653..25618
                     /locus_tag="ACG86D_000522"
     CDS             24653..25618
                     /locus_tag="ACG86D_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3139697.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25675..25905)
                     /locus_tag="ACG86D_000523"
     CDS             complement(25675..25905)
                     /locus_tag="ACG86D_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN3139698.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25991..26878)
                     /locus_tag="ACG86D_000524"
     CDS             complement(25991..26878)
                     /locus_tag="ACG86D_000524"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3139699.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26967..28190)
                     /locus_tag="ACG86D_000525"
     CDS             complement(26967..28190)
                     /locus_tag="ACG86D_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN3139700.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28207..29253)
                     /locus_tag="ACG86D_000526"
     CDS             complement(28207..29253)
                     /locus_tag="ACG86D_000526"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN3139701.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29417..31144)
                     /gene="ptsP"
                     /locus_tag="ACG86D_000527"
     CDS             complement(29417..31144)
                     /gene="ptsP"
                     /locus_tag="ACG86D_000527"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN3139702.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31144..31410)
                     /locus_tag="ACG86D_000528"
     CDS             complement(31144..31410)
                     /locus_tag="ACG86D_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN3139703.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31889..34123
                     /locus_tag="ACG86D_000529"
     CDS             31889..34123
                     /locus_tag="ACG86D_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3139704.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34271..34591
                     /locus_tag="ACG86D_000530"
     CDS             34271..34591
                     /locus_tag="ACG86D_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN3139705.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34796..35020)
                     /locus_tag="ACG86D_000531"
     CDS             complement(34796..35020)
                     /locus_tag="ACG86D_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFN3139706.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35218..36798)
                     /locus_tag="ACG86D_000532"
     CDS             complement(35218..36798)
                     /locus_tag="ACG86D_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN3139707.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37199..38575)
                     /locus_tag="ACG86D_000533"
     CDS             complement(37199..38575)
                     /locus_tag="ACG86D_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN3139708.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38703..39791)
                     /locus_tag="ACG86D_000534"
     CDS             complement(38703..39791)
                     /locus_tag="ACG86D_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3139709.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39963..40397
                     /locus_tag="ACG86D_000535"
     CDS             39963..40397
                     /locus_tag="ACG86D_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139710.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40475..41008)
                     /locus_tag="ACG86D_000536"
     CDS             complement(40475..41008)
                     /locus_tag="ACG86D_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN3139711.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41102..42280)
                     /gene="mutY"
                     /locus_tag="ACG86D_000537"
     CDS             complement(41102..42280)
                     /gene="mutY"
                     /locus_tag="ACG86D_000537"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN3139712.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42273..43070)
                     /gene="recX"
                     /locus_tag="ACG86D_000538"
     CDS             complement(42273..43070)
                     /gene="recX"
                     /locus_tag="ACG86D_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN3139713.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43177..44559
                     /gene="rlmD"
                     /locus_tag="ACG86D_000539"
     CDS             43177..44559
                     /gene="rlmD"
                     /locus_tag="ACG86D_000539"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN3139714.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44733..45311)
                     /locus_tag="ACG86D_000540"
     CDS             complement(44733..45311)
                     /locus_tag="ACG86D_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN3139715.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45633..46103
                     /locus_tag="ACG86D_000541"
     CDS             45633..46103
                     /locus_tag="ACG86D_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN3139716.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46211..47437)
                     /locus_tag="ACG86D_000542"
     CDS             complement(46211..47437)
                     /locus_tag="ACG86D_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3139717.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT        13558 a        10098 c         8220 g        15678 t
ORIGIN      
        1 aacactttga tggatcattt atttgtggaa aactacgttt tgttattcta aaaaacttat
       61 ataaaaaata gtaaggtttt tatttcgaat ccataaaata ttcttggatt gcttctcgtg
      121 tttttttcac tttttcgcta agcttttcgt ttttagccat atagaaataa aaaatgatat
      181 cgatcgtagc aaattgggcc aaaagtgaat tagttgccgc acttcgattc gtgatttctt
      241 ttggtctaga tgtttgtacg agtacatcgg ctatcttgct aagcgggtta tttccaaatt
      301 gcgtcaacgc aatgatttcg atattcatgc ttttcgccag ctcggctaat gcgactacat
      361 ctgcacttct acctgagttt gaaacaagcc aaagcacttt ctttttctca ttgctgacaa
      421 gttgcgggag caagacgtgg atatcttttt caaaaatgat tggcttaccg acacgagacc
      481 atttttgcag aatatcttct actactaaag aagaggcgcc aaccccatat aaaaaaacag
      541 tctctgccga ttccaaacac tctacgactt tttggatcgt ttcttcttct agaatatcta
      601 ctgtttcttt tatcgctact tgtgcattgt taccaagttt gtttttgata gagtaaaagg
      661 actcatttgc ttctatatca ctaaaacctt catcgattcc agtctgtagt tctgttgaaa
      721 tcgtgatttt gaagtccgtc agactttgaa aaccgatttt tttggaaaaa cgtacaactg
      781 tcggcgcact caacccggaa gcttctgcta attcattagc tgtcattgta gggaccttct
      841 caatattacc gataatgtag ttggccaact ttttttctgc tgaagaataa tcttccatca
      901 tactttcaat acgactaatt acgctcataa gttcctccac ccttttaatg atagtctatg
      961 taagtatacc atatcagacc cttattcacg gtcgtatact ggataaaaaa caaaaaaaca
     1021 atccgcccaa cagaatatct tatcccatca atcccacaga ttctattgag catctgtgtc
     1081 tttgaggaat acctgttatc tattgttcgg attgctttct atctactact tttttacagg
     1141 aaatttacct attagttttc tctgctgtaa tttttgactt ctgcttccat agcatcaata
     1201 aagatattca aatcttctac agctgtttct ttacttccat aacgacgtac gttcactgtt
     1261 gcatcttcca attccttgtc tccaacaact aattggtaag ggatcttttg tgtttgagag
     1321 gcacgtatct tatagcccat tttttcatta cgatcatcaa cttctacacg caaccccttc
     1381 atttgcaaac gttctttgat ttcataagca taatcgccat gtgcatcaac agaaacaggg
     1441 atgattgttg cttgtatagg tgccaaccat gttggaaaag cgcctttgta tacttctgtt
     1501 aaataagcga caaaacgttc cattgtagaa acaattccgc ggtggataac tactggacga
     1561 tgcgtatttt ccccgtcttc tccaacatat gtcaaatcaa agcgctctgg caataagaag
     1621 tctaattgta tagttgataa tgtttcttct gtaccaagag ctgttttgac ttgaacatct
     1681 agttttggtc cgtaaaaggc tgcctcgcct tctgcttcaa agtaatctag ttctaattca
     1741 tccatagcgg ctttcagcat tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat
     1801 ttatctgtat tgtttggatc gcgatagctc aagcggaaac gataatctgt aatattgaag
     1861 tcagcatata ctgccaccat caattcaagc gtacgtttaa attcatcttt gatttgatca
     1921 ggacgaacaa atgtatgacc atcattcaac gtcatttctc gtacacgttg taaacctgac
     1981 aaagcacctg atttttcata gcgatgcatc attcctaatt cagcgatacg aattggtaat
     2041 tcacgataag aatggatcgt gtttttgtag accatcatgt gatgagggca gttcattggg
     2101 cgaagaacaa gcatttcgcc atctcccata tccattggcg ggaacatgtc ttcttgataa
     2161 tgatcccagt gtccagatgt tttgtacaac tcaacatctc ccattattgg tgtatataca
     2221 tgttggtaac ctagactaac ttctttgtct acgatataac gttcgatcgt gcgtcggatt
     2281 gttgcccctt ttggaagcca gaatggcaaa ccagaaccta cttctgggga aaccatgaac
     2341 aagtcaagtt cttttcctag tttacgatgg tctctttctt ttgcttcttc acggagacgg
     2401 atatattctt tcaaagcttt cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt
     2461 tgattgtttg agtttccacg ccaataagca cctgccactg ataataattt aaatacttgt
     2521 atacggccag tggatggaac atgtggtcca cgacacaaat cgatgaattc gccttgttga
     2581 taagcagtaa tcacttcttc ttctggcaat tcactaatca gttctacttt gtacggatca
     2641 cttgcaaaaa tttctaatgc ttcctgtttt gaaagaacac ggcgttcgat cggcagattt
     2701 tctttgacga ttttcatcat ttctgcttcg attgccggca aatcttctgc agttacttgg
     2761 ttttgtccat tatctgtatc gtagtaaaac cctgaatcga tagctggtcc aacgccaaag
     2821 tggatatttg gatagaggcg acgcatggct tgggccatca aatgagctgc tgaatgacgg
     2881 actaagccaa gtgcatcttc atggtctggt gtgatgatct caatacttgc atcttcctcg
     2941 attggacgag tcaaatcaac gagttttccg ttcactttac cagctaaagc tttttttgct
     3001 aagctttttg aaatgctttc agcaattgcc aatgttgttg ttccagactc aaattctttg
     3061 actgcaccat ctggaaaagt aatttttatc ataatagttt cctccttgaa atgttttgat
     3121 tgatcagatc atcaagctga aaaacaaaaa aatcctagca tctcttgaaa agagatacta
     3181 ggacgaaaag ttcgtggttc cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt
     3241 aacggtccga ccgcccttgc ttcaacaagg gtttcgaaag tggtcatcct ttggttttct
     3301 cctggaaaag tttcagccta gcttttcctc tcttatcgaa gactctcaaa ttcagttggc
     3361 tttctcatca atcaattatg tgtttattta accactaata aatagaaaaa tcaagtggga
     3421 aactaaactt attttttcca ttccccattt acaccgtatg ttcctttttt catctcattg
     3481 atgatgacat ggatgtgttc ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata
     3541 tctttcatca tattttctaa ttgttccgga ctgcgacctt ctactaattc gacatgtacg
     3601 aatggcataa tgctctctcc tctttctgat cgttcttttt tcaactcgat tctatcatac
     3661 tatgtttgtc ttgggtactg aatcaggaaa aatggctgga tctttttttg ctctcaaggc
     3721 actcaaaata gagaccgtat ccacgacttc ttgcaaaaca gctccgaata atgcggggat
     3781 tagtcccgta cttgcaatta acatcaaaaa aatacaaata aagatgccaa tcagtacaga
     3841 ctgtttcgct attctcatcg tatctcttga aatcactact gcttttgcta cacgcaagag
     3901 atcatctttc aagatgaccg catctgcact ttcactagca gctgtagaac catgagcccc
     3961 cattgcaata ccgatatctg cagcagcaag tgctggtgcg tcattcacgc cgtctcctac
     4021 catcgcaaca ggtctttgat ctttagggat actttctaaa atggtcagtt tatcttgagg
     4081 caagctacgg gcatgtacag aatctaattg gactttttct gcaatcgttt gagcaatggc
     4141 agatccatcc ccggtgatca tcatcaaatt tttcacatca agttttctta gctccttgat
     4201 tgtctttgct gcttctgggc gaatctgatc cgaaaatagg atcgttcctg caaaagatcg
     4261 atcaattgtt acatagattg ctgtattgga cgtttctatt tcttcagctg aaacaaaaga
     4321 ggcacgtcca atctttactt catgattatt gactactgct tggattcctt gaccggtcac
     4381 ttctttcagt tgactaacag gatacagttg gctgtttttc acgttgttca ctaacgaacg
     4441 agctaagata tggctagatt cctgttctgc actagctgca tacagaagta gctcttcttt
     4501 ttttattcca ttgactggct gaatcgcctc tacttccaat atccctttgg taagcgttcc
     4561 tgttttatca aaagcaatcg tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt
     4621 tacaacgatc ccgtttttac ttgaacggct cattcctgca actaaagcaa tgggcgcagc
     4681 tagaatcagt ggacaaggtg atgccactac cagtacctca gcaaatcgta aagggtcttt
     4741 tgagaaccac caagaaatac caccgatcaa ataagcaatc aacgtgaaag ggactgcata
     4801 acgatcagct aaacggacaa atcgggcagg cttttctttt gattcttcga ctagtttaat
     4861 aatcatttga tactggctgt ctgcggcacg tttttcagcg atcatttgga tcgatccgtc
     4921 tccatttaaa gaccctgaca taatctgatc tccaggtttc ttggtttccg ggcgggactc
     4981 acctgtcaaa gaagattcat cgaccgaaga ttctcctttg gtcactcgac catctgcggg
     5041 tacaagttcg ccaggcttga ctagtaactg gtcatcaata tttatttcat cgattactat
     5101 atcctgcaat tttccttctt tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga
     5161 ctgcagttct ttacccgctc gtttattcgc gtaatcctct agactatctc cgcccgtaag
     5221 catgatcaat atcatcaaac tagcccagta ttctttgacg gctaatgttg caaggattgc
     5281 ggtaatcgct aatacatcta cgccatatct tcctgaacgt aacgtcttga tcatttccaa
     5341 aaacatcaag aaggcgagaa tccctccagt aaatgcgaca atcgcaaatg caagggccgg
     5401 ctggtgaaaa acaaattcac ttaataaagc tatcccacca ataagcaaag taagtaaaaa
     5461 ttttttgaaa tgtgtcattg ttttttctcc tcttttatgt gacttagaga ggttagtaat
     5521 aaatttccag ctgcctcttt catcttaagc atagtccttg actagcactt tttaaaacaa
     5581 taggccttaa ttgaaaacaa ctatcactat cagcgtttcc aaaattcatc agttctacaa
     5641 acaaaaatat tcagcaagga ttactttttc gattcatcag ttaaaaagta ccttttttcg
     5701 aaaaactatg ctatactttt aattcgttat ttacggcgga aggagcaaac atgcgatgaa
     5761 tgagaaacaa ctagaacaaa cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca
     5821 aaatatgctg gatacaaaac aacaagaatt gactcaggaa atgaatcatt cgacaaaaga
     5881 cacagctaat cacaagattc gagctggttc caatgaagct ttttatgaat ctgctgttga
     5941 atatcgccaa catgagcagg aattgctttt gaaataccat actcttgaag cacagcaaaa
     6001 acgcctgcaa acgttgtctg tcatgaaaga tagtccttat tttgcccgta tcgactttaa
     6061 agaagaccgt gaaacagaaa cattatatct aggtatcgct tctttaagag atctggcaga
     6121 agaaacaatc gttattgact ggcgcgcacc aattgccaat ctttattatg aaggtgagat
     6181 tggtcccgca ttttatgaaa ctgaaacaga tagaatagat gtcgatctgc agttgaaacg
     6241 acaatttaaa atccaagaag gacgtatttt atctatggta gatacgtcgg aagtaatcaa
     6301 tgatgacttt ttgctagaga tattggatga atcatcaagc aaccatatga aaaacatcgt
     6361 ctctacgata caaaaagcac aaaatgcaat cattcgggac acaacaagta aagtaatgct
     6421 gattgaagga atcgccggaa gtggaaagac atccgctttg cttcaacgta ttgcttttct
     6481 gttgtatcat aatcgcaagt ggctcgaggc tgaaaacgtt cttctatttt cacccaacca
     6541 tttgttttct gattacatct cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac
     6601 acagacattc aaaaattatt tggtgcagct gctgccagag ttttctttac tagatgaaca
     6661 gcaacaagaa agtggttttt tgctagggga aaaagatccg atacaagtga tgaaaagcgg
     6721 attgacctta gtcgatcaga ttgatcgcta tatccaaggc atcacaaact acggtccctt
     6781 gttccgtgat atgaaaataa atggccgtac gatcctatca aaggaatcga ttcgccaatg
     6841 gtataaagaa acaaatgaac aattaccttt acatcataga ctttctctat tacaaacgaa
     6901 gctcctgaaa aaattaggcg gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga
     6961 tctggcggaa gaacagctcc aagaactata cgcaacagat ccaaatcttg aatatacaga
     7021 acaaaaggag cgttcattac gtaaaaaact ggccaaccaa atcgtacgta aaagattccg
     7081 tcgaatccat cgagggatca tgaattatca atttgtcaat ataacgaaac aatatctcca
     7141 ctttctacaa actgtgccac tttctcttct aacacgacat aaaatcagcg agaccaggtg
     7201 gcaggaacat ttgacaactg ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga
     7261 tgctgtacta ttctttttac tgatgcgccg aacatatcca gtgacggtta cacacaaagc
     7321 tcgctatatc tttattgatg aaatgcagga tttcgcacca tcacaagtgg cattgctgca
     7381 agccttgttc ccaaaagcaa atttgacttt ctgtggagat ctaaatcaaa atgtctttgg
     7441 aaatgaaacg atcgttggat cattggaaga ccttttcgtg gaccgtgaag tcactcgttt
     7501 ccaattaacg acaagctatc gctccacaaa agaaatcact gattttgcta atcattttat
     7561 tgtgaaagaa catcaagtag aaacaacagc aagaaaagga gaccttcctt tgctcatcca
     7621 aggaacaaca gatgatatca aattgaattg gttagcagat accattcgaa cgactcaggg
     7681 aaaagcccgg tattggcgta cagcaatcat agctaaaaca caagaagaat gcgaagctct
     7741 ttatgctcaa ctgccagacg agttgaaaca acaagtccag ctgattttaa atgagagtga
     7801 ctttatgaag cgttccatta ttttacttcc agccttttta gcaaaaggtc tggagtttga
     7861 ccgagttttc ttgtgggatg tagatgcaga acatttttct tctcagcaag acaaattgat
     7921 cctttacacc atgtgtacac gtgcgatgca tgaattgacc ctttttacct ccaataatga
     7981 aagtcctttg ttaaacaata tggatatttc atcttatgag aaaaaagtac tgtaagtaaa
     8041 aagacaactg ctatcattaa tgttagcagt tgtcttttta tttacaataa taattgcaga
     8101 aaaagtaatt caatcattta aggtcattta ttctatatat aaatccaaat ctgtttcagg
     8161 atattttaat ttgacaagcg aaataatcat tttcagttct tccatgtttt ctggatattc
     8221 tgggtcaaat accttgctat gacggccaat cccgccattg tcgatcaatc ggtaaattcg
     8281 attccattca atgaattggt caattgattg gaaattgctg attggatagc cgccacaatc
     8341 caaaatctct gatacacgaa atgctaggta tgcactatcg atcactttat tttttttcgt
     8401 gtctatcttc tcatttcttt cgataagaat tttttcagga cgatgtttca agctccactc
     8461 taatagtaga aagaaagcct ccatccgata ctcttcttca aataaccgaa aggctgaatc
     8521 agaacgacga aaaagcagcg gaatcagata attttgtcta taaatcagca ttggtgcgcg
     8581 gtccaccata cactctaaat aacggttcag ataccctgta ccgacatagt ttcctaatcg
     8641 atagccttca acgccgaatc ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt
     8701 cacagctaac tcttctactt tgcggtaacc gcttaagata cgttcaaaag gttctaaagt
     8761 gatttttgct tccatattct tccccgtcca tcttccacat acttcatatc atttcacatg
     8821 atacttatgc tctcatcata ccacaggttg aattagaaaa gaaaataaat cacacaaaaa
     8881 agcaagacaa tctgttagtg agattgtctt gcttttcgaa aaagactgaa tacttttcgt
     8941 taaaactggt tgcttagtct tctaatgcca cattatggaa cacatgctgt acatcttcta
     9001 gatcttccag tgcatcaatc attttttcaa atgtttcttg atcttctgga gatagtgtta
     9061 cctcgttttg agggatcatt tggatctcag cgattgaaaa atcagtgatt cctttttctt
     9121 ttaatgcttc ttgtatacta tggaaatccg ctacttcacc gtaaacgatg atttgtcctt
     9181 cttcttccat tacatcgcga acatcgatgt ctttttccat taggtattca aggatctcgt
     9241 cagcgtcttc tcctgcaaaa gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc
     9301 cggaaacacc catgttcccg ccatttttcc caaatgctgc ccgtacgtct gctgctgttc
     9361 gattcacatt gttcgtcaat gtatcaacaa tcaccattga gccgcttggt ccaaatcctt
     9421 catagcgcaa ttcagagtat gtttcatctc ctgcaccttt tgctttttcg attgctcggt
     9481 caataatatg ttttggtaca ttataagttt tagcgcgatc aatgacgaaa cgtagttttt
     9541 ggtttgcatg cggatcagga tcacctgatt ttgctgccgc gtagatttca atcccaaatt
     9601 ttgcataaat ccggctatta ttagcatctt tagcagtttt ctttgctacg atatttgccc
     9661 atttacgtcc catattttca cttcgctttc tgatttgata gtttcataac gcaaaaacgt
     9721 cctgttccat tatacttgtg aatcacgatt ctgtgaagct ttttttcttt cttccttcat
     9781 tcttctcatt tttagtcaat tatctatact cggattggga tttgcccgaa attctgttcc
     9841 ggaatattgc ctcccaattg atagatttga tcacttctaa ctaacagttc cgcttcttgt
     9901 gcctcttttt ttccgattcg ggaataaata gaaatctctt ttttttcttt taagaatctt
     9961 cttccagcat cggtaaatcc aagtagtcga agataatttt tattttgttg gacaggaatc
    10021 tcttgctttt tgatattcag caagacataa caagataatc gttggatccg tgtccatgta
    10081 tatctttttg tttttacctt tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct
    10141 gttttcatgc gcgattctat tccttcggtc atctgataga tatctgtcag ttccagcaaa
    10201 gtagaagata acaaccgata acgcaaatac ggccaataag attcccaagt ctgcagcgaa
    10261 caagttgaaa gatcttcttc tgtctgaggc ggcacaagtc cagctattct ttttccttct
    10321 tctaacgcat ggcgaatggc tgttgcactg gctatcctct gatctgtgat gattttttca
    10381 tgatagcctg cacttttacg tcggatcgga tgtaattcca tagggtggac atacttggca
    10441 ttttcctttg cataacttaa tcccaagata tgatttggcg aagaaaaatc aagcgtaata
    10501 tcgggataca gactgcgaaa aacctcagtc attttttgcg gataactata cgtttgatca
    10561 gacagttctt gaaatgcctc ttctatccta tcttgattct cttggacaaa gcgtccgaaa
    10621 ctgctgtaat caaacgaagt ctcttcttct gttccaaaac acaaactgtc acagtccagt
    10681 gtttgcaaga tttttactgc acctttagca aaatagtcag ctgactgaac cgcccattcg
    10741 acgggaagct ccactactaa atccacacca ttttgtaaag cttcattcgc gcgatgccat
    10801 ttatcgatga tagccggttc gcctctttgc aaaaaattcc cgctcattac agcaatgacg
    10861 acctctgctc ctgttatttt tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa
    10921 ggattgtact ctacgataat cccgcagctt ttcatgcgca tcctccttca tagttatcga
    10981 taaaaaagaa atagagacaa gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata
    11041 taaactattc tcgcaaacac aatacttctg cgacgaaaag ctgcactgca agagcaatgc
    11101 ctatatcgtt cggatatata atttatgttg aagatttatt cttttcttgt taactcgagc
    11161 aggcaaaacc gctagtcttt gtatgctaca aagaaccagc gctttgattt ctctgatgct
    11221 gcttcgtctg taaaatcaga aaatgcttcg actttcgaaa agccgctgtt ttccagcatt
    11281 cttaaataac tttccaatgg ataggtacgc tcttgatgta gttcatcttc acgaataaat
    11341 cgaccagacc cttcttgttc ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc
    11401 tcgcttcctg gataactatc ccacaaaaaa gcaaaattat ccccttgata atgatagcta
    11461 tattctggaa aaacctcatc tacttgatag gtggagtgta cgtcaaataa aaatcggcca
    11521 ttttcttcta acgcttgata gacttcatca aacacttgct gtacctcttg tctatttttc
    11581 atatagcaca gcgaatcaga aaaacaggta attgcctgat attgtccgat ttctgataaa
    11641 tcgagcatat tgccttccac aaattggatc gatgcgtctt cttcatatgc tcgctggctg
    11701 gcaatcatca acatctcttc tgaaaggtct aaccctgtca ctttatatcc atcttttgca
    11761 aaggcaaccg ctaatgctcc agtaccgcat gccagttcca aaatattttt tgtgtcttct
    11821 tgtaaatgac gtttcgaaaa agctaaccat tgctgataaa gtgtttcgtc catcacttca
    11881 tcgtaaatga aagcaaatgt ttcataagcc atatgttaat ccaaccattc actaagatcg
    11941 actagcggag catctgacca tagtttttcc agattataga aatttcgttc tggcgcatgg
    12001 aaaacatgta cgatcacatc tccaagatcg atcaataccc attttccgcc ttcttttcct
    12061 tcaatacgtt ttacttcata tttgttttct tcttctttat caatgatttc ttctgtaatt
    12121 gcgttgattt gacgttcgct atttgctgaa cagatcatga aataatctgc caacaaggaa
    12181 acatttcgaa cgtctaaagc gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc
    12241 gcaattttta attgttcttt actatctatg atgatttcct cctaatatga gcagcttatt
    12301 tttttgctac ccagtaatta tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg
    12361 caagcaaatg ggccaatgta tgttttgttt caaatgctac agcttcgtct agatcagccc
    12421 atgcaatggc tcgtgcatct tgtaccccag gaaaatctcg acctggttca atatagtctg
    12481 ctacataaat gattttgtca aggagactca tcttagcagc cccagtcgta tgcacccgga
    12541 ttgcatgaag aatctctgca tcggtgattc ctaattcccg ctctacaaac gaagcgccaa
    12601 caagaccatg ccagattgca ttattataat tcaataactc cggatcaaaa ccgtctctta
    12661 cgattaccat tttgaactcc tcatctggtc gttcttttgc ataatcatgt gtcaatgcag
    12721 caatactagc tttttcagga gaagcaccgt attttttggc aagagccacg gctgtttctt
    12781 ctactccaag cacatgcttg aagcgttgtt cgctcattct catctgtatt ttttgcatca
    12841 ggttttctct agaataagca gtatattcat tactatagtc catattcata caacccttca
    12901 ttttgaatat aatcaattac tttatctgga acaaggtagc ggatagaaca gccttctttg
    12961 atcttttgac gtattttcgt ggaactgatg tcgatctcag gtacatctac ccaaattaca
    13021 ggatatggag tatccgttgt gtatcctggt cgacgtatac ccacaaagtt gaccatagaa
    13081 gtcagctcat cgattttata ccattttggc aaatattcta ccatatcccc accgataata
    13141 aagtaataat ctgtatccgg attgttttgc gtcaattcct tcatagtatc ataggtgtaa
    13201 cttttcccac cacgagctaa ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc
    13261 gctaattcca acatgttgag tcgatgtttt gcatcaatcg tctgtttctc atcaacatga
    13321 ggtggctgat aagaaggcat caaaaatacg cggtcaagac ctaaattccg gccagcttgt
    13381 tcagccatca ctaaatgtgc aaggtgaacg ggattgaatg ttccgcccaa gatccctact
    13441 tgctttttct tctctaagaa ttgaggcatt tcttcaggaa ataattgtga acgaacaaat
    13501 gtcttagctt gcgtattcat catggtcact ccttatattg cttttacttc ttttgatagc
    13561 ctttgatact tttcttttgt ggaaggtttg tataatacaa taacacgtcc aatgatttgg
    13621 acgatttcgc aatctatttc tttttctaat acttgagctg cttcttctgc aatctcatct
    13681 gtattttgaa gtaacgatac tttgatcagt tctctttttt ctaaagcttc ttcaatttga
    13741 acgatcattg ctgaattgat cccacctttt ccgatttgaa aaatcggctg aagatgatgc
    13801 gctttgctac gtaaaaaacg cttttgtttt cctcttaaac tcataatttt ccttcttcct
    13861 taaatcaatg ctttgcgttt caatacatct acaccctttg gcgcccagcc cgcaattact
    13921 actggcgctg ttacagtgat ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc
    13981 acagaaaact caaaacgaac aagctccgga aatgcctcga cttcatcagg acgaggaggc
    14041 tgtaataatc cacctacatg tttttgataa aattcgtctg ctttttccag tttcgttcga
    14101 tgaatattta catcatttga cacataagca ataaatgaag cacgattgcc gctgataaaa
    14161 tcaaatcttg caagtccgcc caagaaaagt gtttgccctt cattcagctg ataaacttta
    14221 ggtttgattt cttttaacgg tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc
    14281 atttgatggc gatggataat tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga
    14341 atctcgattt tatccaaggt cgttcctgga aactgcgaag ttgtgattac atcttgtact
    14401 cccgctgtat ttttgatgat ttgattgata agcgtcgatt ttccgacatt tgttactccg
    14461 acgatataga catcccgtcc atcgcggtat tcttcgattt tttctaatag ttccgtcatc
    14521 tcatgagatt tcttagcact agttaataat acatctactg gacgaattcc tgcttcgtaa
    14581 gcacgttcct tcatccattg tgttaattta gtttttttca atgatttagg taatatatcc
    14641 actttgttcc caactaaaag tactggattg tcaccgacaa aacggtgtag tcctgggatt
    14701 agtgaaccat tgaagtcaaa aatatccacc acattgacga tcaatgcatc tttttgccct
    14761 aattcattca gtaagcgcaa aaagtcgtca tccgtcaatg agacatcttg ggtctcatta
    14821 taatggcgta atctgaaaca acgctgacaa tagacttccc cggaagctaa acctttttcc
    14881 aaagccgttt gcggcgtgta tcctaattca ccgggtttgt ttgtttgtat aacggcacca
    14941 cagccgatac attgtaattc ttctgtcatt cgattccacc tcgccagccc atatcagggt
    15001 gttttgctaa tagataggcc ataattcttt tttcccaaaa acgattaaat tgtgtcttcc
    15061 aactatctgt tgcgataatg ggttttacaa ggatgctacg tatccctgca cgattggctc
    15121 cacgtatatc agtcataatc tgatcgccta ccattactat ctcatcatct gctaaatcca
    15181 attgtttctt tgctatgttg atccctcttg ctaaaggttt cattgctcgt gcaacataca
    15241 aaagatcaaa tttttcaatc gctcgcgcga cacgttttga attattgttt gaaacaacaa
    15301 caacagggat tcctgcattt ttcatttcta aaatccaagt aagcaactct tctgttccat
    15361 ctggattatt ccatgcaatc aacgtattgt ctaaatcggt caatactgct ttgatgccta
    15421 gattctttaa ttgggcaggt gtaatctgat agatggcttc taccatccat gttggtttgt
    15481 aatttgaaaa catgctgtct cctcatctgc aaaaaatgaa ggagcacata aagtactcct
    15541 caaccccgta ttatactgca tatcagcaaa aaaaactacc tctatactcg ttcgtctttc
    15601 agaaaaagca tttctttaca aaatgaccaa aaataaagat tgatcacaga aaaatttaga
    15661 aacaaaaaac aactaatatt ggcggaacct cttgtatcgt ttttccagta atttatgctg
    15721 atctaatcga acaagttccg tcaatctttc aatcaacgct tttttcataa tgtgtgcgac
    15781 tttatatcgt ggcaactgct cgccgttgaa cgtttcagga ataactcgat caacgattcc
    15841 taactcgcgt agttctggcg cagtaatttt catcaactca gctgcttctg cggcgcgact
    15901 accgtctttc cacaaaatcg acgcaaatcc ttctggagaa agcacagcat aaatcgtttc
    15961 ttccagcatc cagacttcat ccgctaaacc tagagcaagg gcaccgccac ttccaccttc
    16021 accaatgatg atggcgatga tcggtacctt taaatcagac atttcgagca gatttctggc
    16081 aatagcctct ccttcgccgc gctcttctgc tcctacaccg caataagcac ctgctgtatt
    16141 gatcaaggta atcaccggtc tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt
    16201 tcgatagcct tcaggatggg gagaaccaaa atttctgcgg atattttcct gcagatcccg
    16261 tcctttttga ataccgatca ctgttaccgg tttgccatct aatcgagcga ttccaccaac
    16321 aatcgcttcg tcatctgcaa ataaacgatc accatggcat tcctcgaaag aatcgaagat
    16381 ctcttcaaaa aaatccaagc ttgtccatcg ttctttagct cttgctaact gaacgacatc
    16441 atgtgctgtc ttatccacga tctatccatc ctttctgtgt atgcaattca agaagcgtat
    16501 ggattttctg tttcaattcc atgcggggaa caatctgatc gacaaaacca tgagataata
    16561 agaattcagc cttttggaaa tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc
    16621 gtcttccagc aaaaccgatc aagctttggg gttctgctag aataatatcg ccttccattg
    16681 caaaactggc agttactcct ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac
    16741 cttcattgct atgccgtttg acagcagcag agatttttgc catctgcatt agagaaaaga
    16801 ttccttcttg catccgtgcg ccacctgaag ctgtgaacaa gacgactgga agactttcct
    16861 ttgttgctcg ttcgaataat ctggtgatct tttctccgac aatcgttccc atacttccca
    16921 tgataaaatt aggatccatc actccaataa tgaagggaat cccctgaatg gttgctttac
    16981 cagtgaggac tgcttcatga agtcctgttt tatcttgcat ttttttaatt ttttccgtat
    17041 attcgggaaa tgctagcgga tctttggtta caagatccgt atcccattct tcaaaacttt
    17101 tttcatcaat aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat
    17161 gagggcatac tttttcttca ccgatgtctt tcgtataaag gatatgtttg caatttggac
    17221 actttgccca catgttatca ggaacagatg gcgagttagc aggtttgctc tctctattag
    17281 gattgatccg aatatatttc tttttcttaa acagagccat ctatgcgcct cctatctccg
    17341 tttctggtgt ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg
    17401 ctatgactgc aggatgacta atcaaatcca tctgaaattc tgcgttcgtg atgattcctt
    17461 ctgtgataaa ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc
    17521 catggacgat tatttttgca atcattgaat cataatatgg cgggatcgta tatcccgggt
    17581 aaactgcact gtctactcgc agtcccatcc cacctgctgg aagcagtaag ttttggatct
    17641 tccctggaga aggagcaaaa tggaaagcag gattttctgc attaatccgg cattcgattg
    17701 cgtgtccttg gaatactact tcttcttgac acacattcaa aggctcaccg gctgctatct
    17761 ccacctgttt ttttactaaa tcgattcctg tgaccatttc tgttactgga tgttccactt
    17821 ggattcgagt attcatttcc atgaaataaa aatcgccttc ttcatccatc agaaactcga
    17881 tcgttccggc attttcataa ttgactgctt tagctgctcg aacagcagcg tctcctagtg
    17941 cttctctttt tgtttgtgag atcactacag aaggagattc ttccagtact ttttgattgt
    18001 tgcgttgtaa agaacaatct cgctcgccta aatgaatgac atgaccaaat cgatcaccta
    18061 agatctgaac ttctatatgg cgagcaggat aaatgatttt ttccaaatac atatcatcat
    18121 ttccaaatgc cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg agctcttctt
    18181 ttgattgaac tttccgaatg cctttcccgc caccacctgc agctgctttc agcatcactg
    18241 gataaccaat tctgtctgct atttccaatg cttcttctac tgtagataat acaccagtac
    18301 ttcctggaat gactgggaca tttgcttctt gcatcaattg acgcgcattg atcttatttc
    18361 ccattgcatc gatcgttgca cttttaggac caataaatgt gatattgcac tcttcacaca
    18421 tggaggcaaa tcggctattt tcagataaaa aaccaaatcc tggatgaatt gcttcggctt
    18481 ttgtcacgat tgcagcgctc agtacttcct gaacattcag atatgaatca acggcttttg
    18541 ccgggccaat gcagattgct tcatctgcta actgtgtatg aagtgcttcg gcatctgctt
    18601 ctgaatatat cgctacggtt tggatcccta gttcgcggca tgcacgaatg atacgtacag
    18661 cgatttcacc gcggttagct atcaatactt tagaaaacat atttcaccta tccaatcata
    18721 aaggttaatt ccgcttcggc tactttttta ccgtctactt tagcgattcc tttgcctatt
    18781 ccggctgaag ctttcacttt caatatctca acttcaaggt ataaggtatc tcccggtgta
    18841 actttcttac ggaattttgc tttgtccaat ccgccgaaat aagccgtctt tcccttgaat
    18901 tgttcaagag aaagtaatgc aactgctcct gcttgagcca ttgcttcaac aatcaatacc
    18961 cctggcataa caggttcttc tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca
    19021 tttttcttag caacgatccg ttctccttca accatttctt ccactcgatc aatcaaaagc
    19081 attggataac gatgtggaat gatttctttt atttcttgta tgttcattgt tttccctctt
    19141 tcctgctttg cttatttttg ctgaatccta aagagtggct gtccatattc gacaactgac
    19201 tcgttttcta caaggatttc tgtgatcact ccgtcggcct cactagttat ctcattcatc
    19261 agtttcatcg cttcaataat gcagataact tctcctttgg caacttgatc acctactttt
    19321 ttgaaagctg gtttgtcggg cgctggttgc agataaacga ttcccacgat tggcgaaaca
    19381 acctcgattc catcaaatac tgccgattct ttttctttca ttatttctgg agcagttgtt
    19441 gcttgttcag gtatgacatt gactggctcc gaaactggca acgtttcgtt tgtttgaacc
    19501 atattcatgc ctcgacttgt tttattttta ttcatataca attcaaattg accttcacgc
    19561 aaatcgaatt ccgttagcga ggattgatca aattgactga ctaattctct gatttcattg
    19621 atatccaaaa ttattcctcc cagcgtttca tgcacagaac cgcattatgt ccgccaaacc
    19681 ctaatgaatt actcattgca taagtaaaac ttgcaggttt gctttcgttt ttgacaactg
    19741 tcaagtcaat ctcaggatct tgtttttcta cattgatcgt aggtgggata aattgatgtt
    19801 ctaatgccag caaagttgcc aaggcttcga tcccgccggc tgctcccaat aaatgtccag
    19861 tcatcgattt cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat gcgatttgga
    19921 ttgcttttgc ttctgcctgg tcattggctg gtgtacttgt tccgtgagca ttgatatatc
    19981 cgacttgttc cggctgtaca ccagcttctt ccatcgcaag cttcattgct ttagatgcac
    20041 cagaaccatc tggagtagga gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa
    20101 cgatttctcc atagattttg gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta
    20161 gaacgcctgc tccttcaccc atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac
    20221 cacgattagg atcagttgat tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca
    20281 tctcacaaat cgttgcttct gttcctccag caagaatgac atcagaatag ccatgtttga
    20341 tgttacggaa tgcttcccca attgaattcg ttccgctagc acaagctgtt acgatagctg
    20401 tacaaatacc ttttgcacct acacgcaaag caatatttcc agctgccatg ttggcaatcg
    20461 ccattggtac gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt
    20521 ggttttcgat tgttggaaga ccaccgatcc cagatccgat gatgacgcca aaccggtctt
    20581 gattcatttt ttctttatct aaaccgctca tctccattgc ttctaaagca gcgtagattc
    20641 catagatcga aaacatatcc attcgtttgc tatctttttt aataaaatat ttatcgtaag
    20701 ggaaatcttt gacttcaccc gctaaggtaa tccctgtttc tgaagcatca aatttagtga
    20761 tcggtgcgat gccattcgtc ccctctttta agctattcaa aaagctgtct gcgtcatttc
    20821 caataggtga agtgactcca tagccagtaa ttaccactcg attcatttgt ttgctctcct
    20881 tttcttatcc gtgcatgacc aatccgccat cgacattgat gacttgtccg gtgatatatg
    20941 gattttgtgc taagaaaact gctgtttgtg caatatcttc tacttgtccg aaacgctgca
    21001 tcggaatttg cttttccgct tgttccttga ctttgtctgc taaaacttcc gtcatatcag
    21061 ttgtgatgaa tcctggcgca atggcattac atgtaatgcc tctagttgca gcttctcgtg
    21121 ccacagattt tgttaatcct actacgccag ctttactagc tgcataattt gcttgaccaa
    21181 tatttccaat caacccagat acactagaga gattaatgat ggccccttct cgctgcttca
    21241 tcattttttt caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg atatccagac
    21301 attttttgaa atcttctgca tccattcgca tcacaagttt gtcatttgtg attccggcat
    21361 tgtttactaa cacatggatc gatccaagct tttcttctgc ttctttgatc atttgtcccg
    21421 ctttctcata atcagagatg tcaccggaaa ctcctacgca ttttacaccg aatgcttcga
    21481 tttcttctat cttttctttt ggaatctctc cgcgaccatt caaaatgata tttgctccag
    21541 cttttgcaaa agctaaggcc atcgctttgc cgattcctct tgtactacct gtgataaata
    21601 cattttttcc ttttacatcc ataacgttcc tccttatagt attgctattg tttcatcaaa
    21661 tgtctgctta ttctcaacac gcagaactgg tattgtttta tcgatttttt tcataaatcc
    21721 gcttaatact tttccggggc cgacttcgat gacttgttcg atgccgattg tttttaactt
    21781 atggatactc tcatagaaac gaacaggttg catgacttgc tgttcaagta gtcccgcgat
    21841 tgtttctttc ttcattattt ccgttgttgt gttgcttatg ataggaaaag aaggttcaga
    21901 gaattgaatc tgacttaggt ccttcgccag tttttttgct gccggttcta aaatagctgt
    21961 atgaaaaggc ccgctgacat ttagagggat cattcttttg aacccttttt cttttaatag
    22021 taaaacagct tcgtctactg ctttttcttc gccaccaatc acgatctgct gaggtgtatt
    22081 ataatttgca ggagatacga tgccgtactt gctagcttca tgacagcttt cctcgatcgt
    22141 ttcaatcggc gcattcatca ctgcaaccat tttcccgctt ccagctgggg cagcttctgt
    22201 catataagcg cctctttttg ctactaacgc aactgcttca gtgaaggata gcgcattact
    22261 agctactaat gccgaatatt ctcctaaact cagtcctaaa gctgcatctg ggatgattcc
    22321 atgctccttc aataagcgat aatacgcgat gctgactgtt agaatagctg gctgtgtata
    22381 ttgcgtctga tccaaacgtt cattttctgt aaagcaaagt tcagccatat cgtagccgag
    22441 aatctcactt gcttcatcaa atgtctgctt tacaacggct tcttcatata aatcttttcc
    22501 cataccttga tattgtgcac cttggccact aaataagaat gctgttttca tgtttttctc
    22561 caatatatcc agaatatatg tttgattctt cctattcaaa aaggccgaga cattcctatt
    22621 tcaggaaatg tcccagcccc caacctaatt ttaatgatta tgcagtttgt ttttctacgt
    22681 aagtcactaa atcttgaacc gttttgattc cgtcttctgt ttcgattttc acgtcaaatt
    22741 catcttcaat ttcattgatg atttggaata aatccaagct gtcagcttct aagtcttctt
    22801 ggatgtcagt agtcaatttg atttcttcag tttcttttcc taattcttct gcgatgattt
    22861 cttgaatttt ttcgaatacc ataataatat attcctccga tattgtttgc tttttatttt
    22921 tataatttaa cagtctacaa ctccatgaga atacttcccc atgttaaacc tccgccatag
    22981 cctgtaaata ttactttctg ctgttttcct aaggtgatct ttccactaga aactgcttca
    23041 tccaataaga tagggatgct tgcagcagat gtattcccat atttatccat attggtcagg
    23101 aacttttctt gcggcatctt tacttttcgg gcaattttct caataattct gaggtttgcc
    23161 tgatgcaaaa gcagataatc aactggtttg tctttcatga cttcttggat attgtttgca
    23221 acgtctcgta cggcaaaatc aaaaatatct ctgccagtca tttgcaaata atacgaacct
    23281 tctgaatctt ctgaatagaa cggattttta ttttgctggt agccagcggt caatgagcta
    23341 cttctggtac cgtcagctgc taatttttct ttcagcaatt ttttctgtgg attcgcttct
    23401 aagagaatcc cacctgctcc atcgccaaac aacacagcag tcgtacgatc agaccaatcg
    23461 attagcttag atagtgtttc accaccaatc accagtccct tagttgagcc gcagcggatc
    23521 attttttcag ccatgcttaa cgcataaaca aatcctgagc acgcagcact gatatcaaaa
    23581 gcgatagcat gagttgctcc caatttccct tgtacttgtg ctgctacaga tggtgtcgtg
    23641 taatcgggcg acattgttgc tactaaaata aaatccaaat ctttagggtt tagatttttc
    23701 tgttttatca atttttccgc cactaaaatg cataaatcag aagtctcctg atttgttaca
    23761 catcttcgtt cacgaatacc cgtccttgac gaaatccatt catcagaggt atccatcatc
    23821 ttggaaagat cattattagt aataatcttt tcaggaagga cgctggctgt tgcagtaatc
    23881 attccaaatt gttccattaa atatcctcac ttatagtctt gcaagaagtc gtgcaagttt
    23941 ttcagtgcat ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa gatgttcttg
    24001 accatttcac gatgaaaatg ctgatggacc cgaaatagta actttccttt tttcgttagt
    24061 cccaacttta caacgcggcg gtcatcttca ctgcggatac gttcgacata gccttttttt
    24121 actaacttgt taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc tttggctact
    24181 tctgaagttg tttttttctt gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt
    24241 gacagatctt tgaattgaga ttttttcagc tcagactctt cgattgtcaa aatatcattg
    24301 aagacactaa caagataatc atttactgtt tttaaatctg gttccatgtt ccaccgtcca
    24361 ctaattagtt tgattatcaa atcttttgat gttcaaacta tatcttaact tttttcaaaa
    24421 tgcaacctta aaagatcatt tttttactat atgagctttt tttaagattt tactcattta
    24481 aaacaactga taaatcagca tttataattt cttttattag aaaaatcatc tatctcacat
    24541 attttgatta tcaaagtatt ttctgtgaac agaactatag aattccctaa aacttgtcca
    24601 ttatttcaaa atttgttata gtctttttat tcaaagcgta aaaggagatc ctatgttaaa
    24661 agaaaaaatg aaaactctcc ccatgctgcc gggtgtctat cttatgaaag accgagatgg
    24721 taaggtcatc tatgttggta aagcaaaacg gctcaaaaat agagtaagca gctattttca
    24781 tcagaataaa cagcattcaa aaaaagtatt acgtatgatt catcatataa cagattttga
    24841 tttcgtcgta gtagacacgg aattagatgc tttattgctc gagtgtcaat tgatccaaca
    24901 ttaccgtcct ttttataacc gacaaatgaa ttatttcagt aattataatt atgtacatat
    24961 taccaataag ggatttgtat taacagatac tcctacggct cgaacatacg ggccttttcg
    25021 tctctataaa aaaatgccgt ctattttgcg tataatggaa gaaacttatc aaatgccttg
    25081 gttgtctgaa attagtttgt tagctttgcg agtccagctt cctgacctac aggaaatgac
    25141 ttttgaacag aaaaaaaagg aactccaagg gctgtttcaa ggccgaaata aaaagctact
    25201 gacttacttg aaaaaaagac aacaacattt catctatcag ttgaatttcg aaaaagccgg
    25261 tatgcttcaa aaagatatcg aattagttac ttattttatt agacggatcc aggaacaaaa
    25321 gcagttttta agaacaccat cgctcacctt cagcatgcca ttagcagcag atgagagtca
    25381 gaaaaagcac tacctcgttt gttatggtca acttgcagaa acaatgatta cctctggtga
    25441 cgtttctcct gacttctatt atgaaaagaa agaagctcat ttatctttga aacgtcaatt
    25501 gtcaaaagaa gaaatcgatc ctgtacaaat cttgatcagt taccgcaaaa aattagaaaa
    25561 agaacagttt gaaattgaac tgttaaacaa aaaagaagct gaaaagcaac taaattagtt
    25621 gcttctcggc ttcttttagg catcttcaac ttcaaatgtc aattatccta aaatttattg
    25681 gattaagtca tagatttcca tcgctacgat atcgatatta tcaaattcgt attgctgatg
    25741 agtatctgtt tcagttaatt caaatgtatc ggtcgcttta tcataagtta ctgtacactt
    25801 ctctacgcct tctttttcaa aacggcgaac ttgcagttca ttatctgctg actcgaccat
    25861 agcctccagt cgttttatga ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt
    25921 tcttatttct gttatattgt atcaaaagca tagcctactt gcattgattt tctctaaaaa
    25981 aactcgtttt ttatgatttt ttttctggta tttttccttc aggccaccaa agtttgatca
    26041 aagcttgggt tccgtcattc tcaaaacctt tatctgccaa ctcttcatat agtttcaatg
    26101 cttgggtagt tgctggcaat acaagatcca ttttttctgc ttcttctaat gcaatcttta
    26161 aatctttgat aaaatgtttg acaaaaaaac caggtgtgta atcttctttt aaaatacgag
    26221 gagaataatt gcttaaagac cagttagctg cacttccacc tgaaagcgtc tcaatcactt
    26281 tctttaaatc aagtccgttt ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg
    26341 tacctgcaat catgatctga ttagccattt ttgtatgctg acctttccca gcagaaccat
    26401 gcaatgtaaa tgtcgtacca aattctttga ataaaggcag tacttgatca taaacggctt
    26461 cttctccacc gaccatgatc gtcagtgtac cattctttgc tcctaagtca ccaccggata
    26521 ccggtgcatc taacgcatct gcgccttttt cttttgctgt ttttgcaatt ttttctgcca
    26581 ggcttggtgt acttgtagtc aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa
    26641 tccccgactg tccatagtag atttcttcta catctttggg gtaacctacc attgtaaaga
    26701 gaatattact ttgttctgct accgcttttg gtgtatctgc ccagatagct ccttctgtta
    26761 ctaaaggatc agcttttgct tttgtccgat tatagacagc tacttcgtgt cctgcttcta
    26821 gtagatggcg tgcgacagca cttcccatga cgcctgttcc gataaaacca agtttcattt
    26881 tttcgacctc ctaaaaatca aaaatccttg cttctatgct agtatacgct agaaacaagg
    26941 attttttcat aaaaaatgtt tgaattttat cttttaaatg aatttaattt tattttgatt
    27001 cgttcaatcg agtcttcttc ttcttttctt cgcatctctt cttcatgata aacaaccatg
    27061 tcttgataaa attcgatcat agcttcgccg aaatgtgtag atgaaatatc ataaagttta
    27121 gcttctaaaa cttttggatc tttaggaact tcactggaaa tataatcaat aagtgctggt
    27181 gcaaagtctt cgtcggtttc aaaagttttc cctaatgaag gatgatcgaa caatcggttc
    27241 aaataagcat ttccttccac gacacaaggt gttccggctg ccatcgcttc tgtataagta
    27301 agcccttgtg tttctgaagt agaagcactg acaaaataat ctgccgcttt ataataaagt
    27361 gctacatcat tgttgtccac ttctcctgtg aactgcacaa attcttctaa ctgcagttct
    27421 gagatcaatt cctctaactc ttctacataa ggtccttttc ctacgataac taatctggcg
    27481 ttaggataag cggagatgac ctgaggaaac ccattaatca aggcttggat atttttctca
    27541 taagaaatcc tgcttaaaga aagaatcatc aaatggtttt cctgaagacc tagtgactcc
    27601 cttaatttgc tgatatcctg ctgagtgata tcagaacgtt tgaatttttc aatgtcgata
    27661 ccagtaggaa tcacgcgaag cggtgctatt acaccatagc tgcgtaatgt atcaataaca
    27721 cgttcacttg gacaaactac acctgtcgta tgatttgcaa ataatctaga caagtatttt
    27781 acatgggatt gcctgatcac ttttccttta ccgatataat gaaggtagtc ttcatacatc
    27841 gtatggtaag tatggatcag aggtattttc aacttcttag ctaccatctt cccaagtatc
    27901 cctgctccga attctgtatg ggtatgaata agatctagtt ctagttcttt ggcaaccaaa
    27961 taagcatacc acatgcctct aacaacaact cttcgatcct tgaaagaaat aaatggtaca
    28021 cttggcattc gaatcacatc tttttccaac ttatccgcat taggatcggt cgttgtaaaa
    28081 atatagactt catgcccatg tctttctaat tcttgtttta atgttttgat cgatgtagca
    28141 actccgctga cttgcggaaa gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc
    28201 tccaaactag tccttgacct gatttacttt ttttaatttg ctagagactt tttcattcag
    28261 tacactttcg tatacatttt taagttcttc accgatttta ttgatacttc gctcttttgc
    28321 tgtttgataa ccggcttgtg tcgtactcgg tagttcttga ttggtaatct gttggatcag
    28381 tcggataaat tcttcgtttg tcttccccat atagcagttc ttgctatctt ctaaccagcc
    28441 attatacact ggaatatctc gaacaagtac gttttgtttg cttgctaaag cttctaaaac
    28501 aacgatccct tctgtctctt cataagaagg gaaaaagaat agatctgcac ctgaatacgc
    28561 accttctatg atctctccac gaatgtaacc aggaaacagg acattttcag gatgatcttc
    28621 ttttacaatt ttccggatgt tccgaggaat cgagtacatg ggaacatgac caaaccaaat
    28681 aaatgtgtat tcgggcatct tcttagcaat ttctacgaaa tcaatgattc ctttacgttc
    28741 gaaaaacaaa ccgacacaaa tgattacttt ttgatccgga gacagtttga aatattctct
    28801 gaacttttgt tctttcattg gatcagggtg atatttctct aagtcgatcc cattggaaat
    28861 agcttgaatc ggcacacgga taccatagct ttccaacaac tgttttgaat aaggtgttgg
    28921 cgtgatcaga tagtccgctt tttggtacaa tcctaccaga tattttttta cgataggaga
    28981 aagctggttc gatctgataa aggaatttcg gaaatcttcc tctgtagaat gcgcatgata
    29041 gacaactttt tttccttctc ttctggcttt attgaccata ttatgactat tgattccgta
    29101 tgtattgatg tgcaggatgt cataatcttc tttttcatct agtgtataag agataccgac
    29161 ttccgtcaga gcacgttttt gatgatcaag cgcacgcccg attccagatt ttgcaagcat
    29221 cttttcactt tcaaaatata ataatacctt caccttacgt cccccttcac ttatatagta
    29281 taacatagcc tacaaactgt cactcaaact taagccctat ttttgtctat tcttactgac
    29341 aaaaacgttt tcttaaaaaa gaacaaaaaa actggcgtag ctcttttaca agccacacca
    29401 gcttttgata cgttgtttat tttacatatt catgaacaag ttcaacgact tcttccatca
    29461 tatcgcattc tttcaatgca cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt
    29521 tcattaaact acgcgtttta agaatagatg tcgcactcat tgagaactca tctagaccca
    29581 tacctactag caatggaaca gctgtttgat ctccagccat ttctccacac atacctgccc
    29641 attttccttc agcgtgtgct gcatcaatta catttttgat caaacgtaaa attgatgggt
    29701 tatatggttg gtacaagtaa gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt
    29761 gaatcaaatc gttcgtacct acagagaaga aatctacttc ttttgcaaac ttgtctgcta
    29821 agactgctgc tgctggaatc tcgatcatga tgccgacttg gatcgtatca gatacttcct
    29881 tgccttcact tatcaatttt tgtttttctt cttcaaagat cgcttttgct gcgcggaatt
    29941 ctttgagtgt agctaccata gggaacatga tacgtaaatt accatgaaca gatgcacgaa
    30001 gcaatgcacg catttgtgta cggaacattt cgtcgccttg ttcagataaa ctgatacgca
    30061 atgcacgata tcctaagaat ggattcattt catgaggtaa ttgcagatat ggtagttctt
    30121 tatctccacc gatgtccatt gtacgaacaa ctacaggttt tccttccata ccttctaata
    30181 cagctttgta agcttcatat tggtcatctt ctgttgggaa gtctggagaa tccatataca
    30241 ggaattcagt acggtacaga ccaactgctt ctccaccatt attatggaca ccaactagat
    30301 cttttggtgt tccgatgttt gcagctaatt caaaatgctt gccgtcagct gtgacagttt
    30361 ctgcattttt taatttttcc cattctgctt tttgtgctgc gtaatctgca cctgcttttt
    30421 caaattcagc tttttgttca tctgtaggat cgatgattac atcgccttcg atcccgttga
    30481 cagccaaaat atcgccttct ttgactttag cagtaatttc ttttgtccca acgattgctg
    30541 gaatttctaa agaacgtgcc atgatagcag aatgtgaagt acgtccacca atgtctgtta
    30601 caaacgcttt tacaaaattg cgatctaatt gtgctgtatc acttggagtc aagtcatgtg
    30661 caacaacgac cacttcttcg ttgatcattg atggatttgg taaagtaacg cctaataagt
    30721 gcgctaaaat acgtttagca acgtcacgaa tatctgcagc acgctcttgc atataagcat
    30781 tatcttccat tgcttcgaac ataccgatat acatatccgt tacttctttt agcgctgatt
    30841 cagcatttac gctgttgtct ttaatatttt gcttgatttg acctaccatt tctggatctg
    30901 aaagaaccat caaatgggca tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg
    30961 ctttttcacg aatttgctgc aattcttcag tagatttagc taatgcagca tccaaacgag
    31021 cttcttcagc cgcagtatct tctactgttg ccttactgaa tgttaaatcc ggttgaacta
    31081 gcaggtaagc ttttgcaacg gctacaccgt cactagcggc aatccctttt agcatttcaa
    31141 ccattattca gataagcctt ctttcttcat tgtttcaacg attgctgcca ttgcatcagc
    31201 ttcgtcagca ccttctgcag taatagtaac atcagaacct tgacctacac ctaaagacat
    31261 aacgcccatg attgatttca agtttactga tttaccttta tactctaagt ttacatcaga
    31321 gttaaatttg cttgctgttt gtactaatag tgtagctgga cgagcgtgga tcccagtttc
    31381 tgctactacg tgaaattctt tcttttccat attaatcggt ctcctttaag tgagtgttgt
    31441 ttttatgtga gggttttctt ttttagtaat tcagagaatc ctaacaaatg ccaccctttc
    31501 atctgtaaag attaacattt tttttcgtta gatacaacag ttttcattca atttcagata
    31561 aaagtattca aattattccg gattcttttt catccttttc ttcacatttt ataacaaacg
    31621 tgtttatttc ctttttcttg tcgctttctc ttctcctgac gtttatcatt ctttcctttg
    31681 accctctttg ttttcatatg aaacgaatta cttgcttccc tttttatttt tagtataata
    31741 agagtcaaag gtcaggaatg gtcaatagag atttcacctt ttctacctca taaatctctt
    31801 aaaaaactag gggttattct tttacctcat gaaaaacgag atgtaaaata tggatgtacc
    31861 catttttgtg aaaggacgtg aatgattcat gctttgtcaa aactgcggca agaatgaggc
    31921 aacgattcac ttgtatgcca ctgtcaatgg tcagcggaca caactcgatt actgtcagag
    31981 ctgttatcaa aaaatcaaaa atcaacaaaa tggaggtctg actaatatgg cccaaaatga
    32041 tccatttgga tttggcagtt tggacgatct tttccgttcc atgtctcgcc aaatgcaaca
    32101 gcaaggaaat tatgagcaaa caccaccaac acaatttggc ggaaacaaca acttcaatgg
    32161 tggtcagcca cctcaaggca atacagaagg tttattggga gaatacggaa tcaatatcac
    32221 agaacaagca cgacaaggtg atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg
    32281 agtcattgaa attttaaatc gccgaaccaa gaacaatcct gtcttgattg gagaaccagg
    32341 tgttggtaaa acagcagttg ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc
    32401 gcaaaaatta atggacaaag aagtcattcg tttagatgtc gtatctttag tccaaggtac
    32461 tggtatacga ggacaatttg aagaacgtat gcaaaaattg attgaagaga tccgtcaagc
    32521 agaaaatgtg atcctattca tagatgaagt tcacgaaatc gtcggtgctg gttctgctgg
    32581 tgatggaaat atggatgctg gtaatatttt aaaaccagct ttagctcgag gagaattgca
    32641 aatggttggt gctactacct taaatgagta ccgcatcatc gaaaaagacg ctgctttaga
    32701 acgtcgtatg caacctgtac gggtagatga accaactgtc gaagaaacga tcagtatttt
    32761 gaaaggtttg caaaaacgtt atgaagacta tcatcatgta aaatatactg atgaggccat
    32821 cgaagcagcc gcaactttat ccaatcgtta cgtccaagat cgcttcttgc cggataaagc
    32881 catcgatttg ctagatgaaa caggttctaa gaaaaacttg acgatccaaa tcgttgatcc
    32941 taaaacaatc gagaaaaaac tgcaagaagc agaagaacaa aaagttttag cttctcgaga
    33001 agaagatttc gaaaaagcag cttattatcg cgatcagatc aataaacttc aaaaaatgaa
    33061 agaacgtcag ctcactgaag aggaaacacc tgtgattacc gaaaaagata tggaaaaaat
    33121 cgttgaacaa cggacaggta ttccagtggg tgaattaaaa gaaaaagaac agacacaact
    33181 gaaaaactta gcagatgact tgaaagctca tgtcatcgga caagataacg ctgtagatcg
    33241 agtagcgaaa gcgattcgcc gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg
    33301 ctccttctta tttgttggac caactggtgt aggtaaaaca gagcttgcga aacaacttgc
    33361 ctacgaacta ttcggttcac aagattcaat gattcgtttc gatatgtctg aatatatgga
    33421 aaaacatagt gtctctaaat tgattggttc tcctccaggt tatgtcggat acgaagaagc
    33481 tggtcaatta acagaaaaag tacggagaaa tccttacagt cttgtcttgt tagatgaagt
    33541 agaaaaagct catccagatg ttttgcatat gttcttgcaa atcttagatg atggtcgatt
    33601 gacagatgca caaggaagaa cggtcagctt taaagatacg atcatcatta tgacaagtaa
    33661 tgccggaact ggtaaagtag aagcaaacgt tggttttggt gcggcacgcg aaggtgttac
    33721 tcgttctgta ttgaaccaat taaacaacta ctttacacca gaatttctga accgttttga
    33781 cgggatcatc gaattcagtg cattatcaaa agaaaacttg atgacaatcg tcacactaat
    33841 gctagacgat gtcaaccaaa tgctggcagc acagcaactt cacatcgaag ttcctacgaa
    33901 tgtcaaagaa aaattagttg acttaggcta tgatccatct atgggtgctc gtcctcttcg
    33961 acgcacgatc caagaacaaa tcgaagatgg tatcgctgaa ttttatcttg atcacccttc
    34021 tatccatgag ttgaaagcaa aactggataa agatggaaaa atcatcgtta cttctaaacc
    34081 agaacgtttg gctaaagaat cagccgaaga aacggctgaa taaatccagt tttatagcgt
    34141 gagacggtat ttctttgaga aatgccgtct cattcttttt tcagaaaagt caaaatactg
    34201 ttcattttat aaaaaagaaa ttataataag agaaacaagc aattaaaaat acataaaaga
    34261 ggtgagcgcc atgaaattgg taaatgtaac aaacagccat tcccgtcttg tattaaacca
    34321 gcttgaaaac actgatgccc atctagtcaa ggtctacaca gccggaaata ccactattgt
    34381 ctacactgaa gcccctgaac ataatgaaat tctgctgatc aatgacaaac gtaaaatcca
    34441 gccgaaagaa atcgaagatg ctaaaaatta tttcttagat aaaatcaaag acgccgttta
    34501 ccacgaggat gaaatcaaag taatcgaatt ggcaggcctt gttgaaatct cgattccaaa
    34561 acatgaatta ggcgctacat tagccgtgta aacaaatttt gaacataata attaaagagc
    34621 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcgaaag
    34681 cagaagcaac tccttcggaa ataagctgaa attcacgaaa attgcttttc aaatttttgt
    34741 gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt tctttctagc
    34801 tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt ctacgcgtgt
    34861 ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt gcaatttgct
    34921 cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc tcttcttttt
    34981 tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact ctcctttctt
    35041 taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc tatgatttta
    35101 atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa tatgagaggc
    35161 tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa gacttcttta
    35221 catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt ctgcaaattg
    35281 gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat tgcgacttga
    35341 actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt gtcccattgg
    35401 agacatgatt acttcggcgt tatactcatt taacatacga tattggaata cttcgaattg
    35461 tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat aaagctgaat
    35521 cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt tcatgacatt
    35581 ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt cataagcaac
    35641 ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat catatacacc
    35701 gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca taaattgtgt
    35761 gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca ttcctcgttc
    35821 gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg gattcatatt
    35881 tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta ccatctgatc
    35941 atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct ctaaaaaagt
    36001 ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat cgccacgagc
    36061 aattttttct tgattaaatg catctccagc ctctttgatt agttctactt cttctaagac
    36121 ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc catcttcatc
    36181 caattctact aatcgttcat cctgataatt ttccggacga taaaactcta cacgattatg
    36241 gtagatatca taaagacctt ctagcccttt acccataccg attggccaat tcatcggata
    36301 agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt cacgaccatc
    36361 acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta caacttggaa
    36421 taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga ccgcactatc
    36481 cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg gtgtatccaa
    36541 gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa cagaaatccc
    36601 acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt ttttgccttt
    36661 tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg taatcgttgt
    36721 tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca cttgttgctt
    36781 taattttgga ttattcataa cctaactcca tttcttccat tcaattattt atattttctc
    36841 taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct gtgacaaaag
    36901 ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta tcagcaataa
    36961 gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta ttacttaatg
    37021 caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat agcatggatt
    37081 cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca gctgactaaa
    37141 agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa gtttccaatt
    37201 atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct tcttcaggtt
    37261 ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt cgagaacctt
    37321 ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt tcgccttcat
    37381 ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca tcactcatat
    37441 gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct tggatgatat
    37501 attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca tcatcgattt
    37561 ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg ccgccatatt
    37621 catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac agatcatcaa
    37681 taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata tctaaattat
    37741 caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt ccgactactt
    37801 tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa acttcattca
    37861 cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt gggaccatga
    37921 cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct aattcttcat
    37981 tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc atttttgaat
    38041 cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa gcagaaagca
    38101 gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa gtgaactttg
    38161 cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca aaaacaatcg
    38221 atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc gctgtgccgc
    38281 cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca ccagacaaaa
    38341 tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga ttctgcaata
    38401 tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg attcgattct
    38461 tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc aatgtcaaaa
    38521 cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca ttcataatcg
    38581 cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg gcactatagc
    38641 cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg tactaaaact
    38701 acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc tttgacgatt
    38761 ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc taagaagata
    38821 ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt taatggatgg
    38881 attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa ctgttggacg
    38941 attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac tgttactaga
    39001 aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt caatcccgca
    39061 atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa aaatgtgaag
    39121 gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa tgttttattc
    39181 agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa ctgttggatg
    39241 tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg tgctgtaatg
    39301 attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt ggatactcca
    39361 ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa atatttattg
    39421 atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct ttctacctgt
    39481 ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag acttgggatg
    39541 acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat tgcgacagcc
    39601 catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa taaatagtaa
    39661 agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc gatcggttcg
    39721 aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa taattcaagc
    39781 gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc tcctttcttt
    39841 cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat ttttgaaaac
    39901 ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa ggtggctaga
    39961 ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat gctgtacgga
    40021 tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa atagacaaag
    40081 atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg caaggaactg
    40141 tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg attctttctg
    40201 aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac tttgctaaga
    40261 atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact ttttacgaaa
    40321 aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct catatttata
    40381 tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag cttttgagaa
    40441 aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat tgcttgtaac
    40501 ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta ttgatccagt
    40561 caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg taattcatca
    40621 ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg tcaggaaata
    40681 ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc aaaaaaggtg
    40741 aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt acattgaacc
    40801 agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg cgtccatcag
    40861 attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt gtttttaata
    40921 ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt tggatcgtca
    40981 caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca ttttgtgctt
    41041 tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa atgagctttg
    41101 cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt ttcatttgga
    41161 ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa gttttgttct
    41221 cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga acatgccatt
    41281 tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa acaaagagtt
    41341 cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca aacagatcga
    41401 tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt gtcacttcca
    41461 tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct tcaaaataat
    41521 acgctccaga atgattttga agagctgcac tgatataata aacgtctttt ggctttgctt
    41581 tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa caaaaggctt
    41641 ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa cctagatcca
    41701 tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc atcgcttcat
    41761 caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac aatctgctga
    41821 ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg atacttgcaa
    41881 tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca ggcgtttgcg
    41941 gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg ttccgggctc
    42001 ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga gcatttgcta
    42061 gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc actgtgtcta
    42121 ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca cgattatagc
    42181 gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct tgaaattctt
    42241 ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc cttttccgta
    42301 atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg ctttactttt
    42361 agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc tccttgtttg
    42421 actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc tatcgcttgt
    42481 tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt tctcaataat
    42541 tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg atgtgcattt
    42601 tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc aggtttcaaa
    42661 cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa gcgcatattc
    42721 gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc ttttaagcgg
    42781 gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta tgttcggatt
    42841 tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa accgaaatca
    42901 gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg tccttttaat
    42961 aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc agataaatct
    43021 acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat aaacatcctc
    43081 ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta ttatatgtta
    43141 gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat tgtcaaaact
    43201 ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg aatcggttat
    43261 ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt actagtgaaa
    43321 atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa aaaaacaagc
    43381 aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg tcaattacaa
    43441 cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca agcattggaa
    43501 aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg gatggatgag
    43561 ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca gactcaaaaa
    43621 attgaagctg gtttatacga agcaaactct catcagttag tccctataac agactgcttg
    43681 gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt gaataaattt
    43741 gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat catggttaga
    43801 atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa aaaattcccg
    43861 caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt ttcaatcatg
    43921 caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat ccacttatgg
    43981 ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc tcctcgtgcc
    44041 ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat caaagcatta
    44101 gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac gatcggctta
    44161 agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca agcaatcgct
    44221 gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga agtcgggaca
    44281 gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg tatcatcgta
    44341 gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa ataccctcca
    44401 aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct ggttaattta
    44461 agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca gactgcacgt
    44521 tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt tgtgaaaaag
    44581 tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat gttttatgaa
    44641 tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa aaagggttgt
    44701 gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc tatcacttga
    44761 taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt atatcctttt
    44821 cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt tgctttgtcg
    44881 tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg attccaagtt
    44941 ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa attccaagta
    45001 atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt ctgttcatct
    45061 aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa tgtatttttg
    45121 gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt taagtttcct
    45181 gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc atgcgcaaaa
    45241 cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa taacgtacta
    45301 atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc tttattgtat
    45361 taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga aaaaagtaag
    45421 atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga gaatcaaatc
    45481 atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt taataacaac
    45541 aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg agccttcatg
    45601 gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat attcgttttt
    45661 gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt agcggaaatc
    45721 ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc tggatatttg
    45781 ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg ttacttaatc
    45841 acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag ttcattttta
    45901 attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca gcaatcgata
    45961 ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg catcatcgcc
    46021 tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact ctattgaatt
    46081 attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt aacacataga
    46141 aaaagtgcca cctaagttag attttttgtc taatttaggt ggcactcttt attaaccaaa
    46201 ctattttttt ctagtaccca cttgttgttc catactcatt attctggtca taaccataac
    46261 cattttgttc ctgtccagcg taaggatcat aactttgagt atcttgactt ggtgtatagt
    46321 actgacttgt atcttctgtt tgctgataat aggtattcgg atcttggtac tgatcatatg
    46381 tgttttctga ataggaagaa taatcggtgt acccttcact attcgatcca tcaccgtttg
    46441 tcgaatcgcc atcttccgaa tcatcaaaga acatcattga agcattctca tttttcaaat
    46501 taggtaatgt atcgcttgtt tttatattta attgtttctt cagttcattt tggatcgaaa
    46561 gaagatcatt ttttcctaaa atttgatagt agatatcatt gatcatttgt ccttcgcctt
    46621 gcaactggtc ttgtttgatt gtttcaaacg ccggggtgta gttcgtcgct acatcaagca
    46681 tgtcatccca atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa atttttctat
    46741 aattgcttac gccatctaaa cttaaaactt tattgacgat tttcgtcact acttcccgct
    46801 gacgacgttg tcgaccgata tcgccttctg gatcttcgtg tcgcattcga gaataggcta
    46861 aacctttttc tccattcaat gtttgttcac ctactggtac atggacacca tccaaagtaa
    46921 agtcaatttt attattcact ttgatgccac cgaccgcatt gatcaaatct tccatgccat
    46981 ccaaattgat cgttacataa tgatcaatag gaatatcgag cagttgctca attgaatcca
    47041 tggccatttc gacaccacca aatgcgtagg catgatttaa tttatctacc gttccataac
    47101 ctacgatatt cgtataaata tctcggtcca aactgatgat cgttgatttt ttttgtttcg
    47161 gattaactgt tactaccatc atcgtatcag aacgtccttg ttccgttctt cctaatccac
    47221 cggtatctaa acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg cttactctct
    47281 cagaacgttt ggaggatatt cgattagagt ttttagaaat tttgtttact gtttggctcg
    47341 cttctcccat aaatctgatt ccgtatgctg ataatccggc aatcagcaaa acgaataccc
    47401 ctaacaaggt taatagaact ttttgccatc gcttcataat acacctctgt ttcacatttt
    47461 ctaaaatatt gtctcatata actgttcaat cagcaaattc actttttaat cataagaaaa
    47521 ctttaaaaaa catttttgtg taacgattat tatt
//
LOCUS       DAZAJZ010000009        44881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000009 DAZAJZ010000000
VERSION     DAZAJZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.009"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG86D_000543"
     CDS             212..604
                     /locus_tag="ACG86D_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3139718.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG86D_000544"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG86D_000544"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN3139719.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG86D_000545"
     CDS             2698..2943
                     /locus_tag="ACG86D_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139720.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG86D_000546"
     CDS             3165..4775
                     /locus_tag="ACG86D_000546"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN3139721.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG86D_000547"
     CDS             complement(4752..4964)
                     /locus_tag="ACG86D_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139722.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG86D_000548"
     CDS             5067..5936
                     /locus_tag="ACG86D_000548"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN3139723.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG86D_000549"
     CDS             6101..7372
                     /locus_tag="ACG86D_000549"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN3139724.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG86D_000550"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG86D_000550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN3139725.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG86D_000551"
     CDS             8763..9026
                     /locus_tag="ACG86D_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN3139726.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG86D_000552"
     CDS             9471..9920
                     /locus_tag="ACG86D_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3139727.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG86D_000553"
     CDS             9977..11395
                     /locus_tag="ACG86D_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN3139728.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG86D_000554"
     CDS             11395..12255
                     /locus_tag="ACG86D_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN3139729.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG86D_000555"
     CDS             12429..13202
                     /locus_tag="ACG86D_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3139730.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG86D_000556"
     CDS             13349..13714
                     /locus_tag="ACG86D_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN3139731.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG86D_000557"
     CDS             complement(13862..15070)
                     /locus_tag="ACG86D_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3139732.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG86D_000558"
     CDS             complement(15089..15367)
                     /locus_tag="ACG86D_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139733.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG86D_000559"
     CDS             15560..16216
                     /locus_tag="ACG86D_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3139734.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG86D_000560"
     CDS             complement(16658..17455)
                     /locus_tag="ACG86D_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN3139735.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG86D_000561"
     CDS             complement(17452..18636)
                     /locus_tag="ACG86D_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN3139736.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG86D_000562"
     CDS             complement(18661..19908)
                     /locus_tag="ACG86D_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN3139737.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG86D_000563"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG86D_000563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN3139738.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG86D_000564"
     CDS             21888..22202
                     /locus_tag="ACG86D_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN3139739.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG86D_000565"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG86D_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN3139740.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG86D_000566"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG86D_000566"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN3139741.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG86D_000567"
     CDS             23546..23875
                     /locus_tag="ACG86D_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN3139742.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG86D_000568"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG86D_000568"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN3139743.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG86D_000569"
     CDS             24863..25687
                     /locus_tag="ACG86D_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN3139744.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG86D_000570"
     CDS             25680..26027
                     /locus_tag="ACG86D_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN3139745.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG86D_000571"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG86D_000571"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN3139746.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG86D_000572"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG86D_000572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN3139747.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG86D_000573"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG86D_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN3139748.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG86D_000574"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG86D_000574"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN3139749.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG86D_000575"
     CDS             31684..32451
                     /locus_tag="ACG86D_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139750.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG86D_000576"
     CDS             32441..34483
                     /locus_tag="ACG86D_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139751.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG86D_000577"
     CDS             34764..34913
                     /locus_tag="ACG86D_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN3139752.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG86D_000578"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG86D_000578"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN3139753.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG86D_000579"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG86D_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN3139754.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG86D_000580"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG86D_000580"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN3139755.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG86D_000581"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG86D_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN3139756.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG86D_000582"
     CDS             39330..40412
                     /locus_tag="ACG86D_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN3139757.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG86D_000583"
     CDS             40412..41215
                     /locus_tag="ACG86D_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN3139758.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG86D_000584"
     CDS             41285..42025
                     /locus_tag="ACG86D_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3139759.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG86D_000585"
     CDS             42036..43040
                     /locus_tag="ACG86D_000585"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN3139760.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44881)
                     /locus_tag="ACG86D_000586"
     CDS             complement(43085..>44881)
                     /locus_tag="ACG86D_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139761.1"
                     /translation="RIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFY
                     TKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKI
                     LYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLI
                     SFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVS
                     EDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGV
                     LLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIH
                     CLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDL
                     LEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLC
                     SFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRET
                     HAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKD
                     VADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14426 a         7832 c         9344 g        13279 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 g
//
LOCUS       DAZAJZ010000010        43984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000010 DAZAJZ010000000
VERSION     DAZAJZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.010"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..822
                     /locus_tag="ACG86D_000587"
     CDS             70..822
                     /locus_tag="ACG86D_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3139762.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            819..1733
                     /gene="pfkB"
                     /locus_tag="ACG86D_000588"
     CDS             819..1733
                     /gene="pfkB"
                     /locus_tag="ACG86D_000588"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN3139763.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1751..3682
                     /locus_tag="ACG86D_000589"
     CDS             1751..3682
                     /locus_tag="ACG86D_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN3139764.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3793..5352)
                     /locus_tag="ACG86D_000590"
     CDS             complement(3793..5352)
                     /locus_tag="ACG86D_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN3139765.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5507..7741
                     /gene="pcrA"
                     /locus_tag="ACG86D_000591"
     CDS             5507..7741
                     /gene="pcrA"
                     /locus_tag="ACG86D_000591"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN3139766.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7758..9794
                     /gene="ligA"
                     /locus_tag="ACG86D_000592"
     CDS             7758..9794
                     /gene="ligA"
                     /locus_tag="ACG86D_000592"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN3139767.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9900..10205
                     /gene="gatC"
                     /locus_tag="ACG86D_000593"
     CDS             9900..10205
                     /gene="gatC"
                     /locus_tag="ACG86D_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN3139768.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10205..11677
                     /gene="gatA"
                     /locus_tag="ACG86D_000594"
     CDS             10205..11677
                     /gene="gatA"
                     /locus_tag="ACG86D_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN3139769.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11674..13104
                     /gene="gatB"
                     /locus_tag="ACG86D_000595"
     CDS             11674..13104
                     /gene="gatB"
                     /locus_tag="ACG86D_000595"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN3139770.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13114..14169
                     /locus_tag="ACG86D_000596"
     CDS             13114..14169
                     /locus_tag="ACG86D_000596"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN3139771.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14280..15656
                     /gene="rlmD"
                     /locus_tag="ACG86D_000597"
     CDS             14280..15656
                     /gene="rlmD"
                     /locus_tag="ACG86D_000597"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN3139772.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15865..16701
                     /locus_tag="ACG86D_000598"
     CDS             15865..16701
                     /locus_tag="ACG86D_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3139773.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            16850..17071
                     /locus_tag="ACG86D_000599"
     CDS             16850..17071
                     /locus_tag="ACG86D_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139774.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            17072..19789
                     /locus_tag="ACG86D_000600"
     CDS             17072..19789
                     /locus_tag="ACG86D_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFN3139775.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            19782..21737
                     /locus_tag="ACG86D_000601"
     CDS             19782..21737
                     /locus_tag="ACG86D_000601"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3139776.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            21727..22863
                     /locus_tag="ACG86D_000602"
     CDS             21727..22863
                     /locus_tag="ACG86D_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3139777.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(22914..23117)
                     /locus_tag="ACG86D_000603"
     CDS             complement(22914..23117)
                     /locus_tag="ACG86D_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139778.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(23133..24965)
                     /locus_tag="ACG86D_000604"
     CDS             complement(23133..24965)
                     /locus_tag="ACG86D_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3139779.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(24968..25414)
                     /locus_tag="ACG86D_000605"
     CDS             complement(24968..25414)
                     /locus_tag="ACG86D_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFN3139780.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(25407..26357)
                     /locus_tag="ACG86D_000606"
     CDS             complement(25407..26357)
                     /locus_tag="ACG86D_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFN3139781.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            26752..27369
                     /locus_tag="ACG86D_000607"
     CDS             26752..27369
                     /locus_tag="ACG86D_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139782.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            27466..27672
                     /locus_tag="ACG86D_000608"
     CDS             27466..27672
                     /locus_tag="ACG86D_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139783.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            27677..27991
                     /locus_tag="ACG86D_000609"
     CDS             27677..27991
                     /locus_tag="ACG86D_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFN3139784.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            27992..29134
                     /locus_tag="ACG86D_000610"
     CDS             27992..29134
                     /locus_tag="ACG86D_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFN3139785.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            29136..29678
                     /locus_tag="ACG86D_000611"
     CDS             29136..29678
                     /locus_tag="ACG86D_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFN3139786.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            29739..31676
                     /locus_tag="ACG86D_000612"
     CDS             29739..31676
                     /locus_tag="ACG86D_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFN3139787.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            31887..32657
                     /locus_tag="ACG86D_000613"
     CDS             31887..32657
                     /locus_tag="ACG86D_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN3139788.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            32650..32994
                     /locus_tag="ACG86D_000614"
     CDS             32650..32994
                     /locus_tag="ACG86D_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFN3139789.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            32987..35230
                     /locus_tag="ACG86D_000615"
     CDS             32987..35230
                     /locus_tag="ACG86D_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFN3139790.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            35368..35577
                     /locus_tag="ACG86D_000616"
     CDS             35368..35577
                     /locus_tag="ACG86D_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139791.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            35574..35858
                     /locus_tag="ACG86D_000617"
     CDS             35574..35858
                     /locus_tag="ACG86D_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFN3139792.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            35858..37210
                     /locus_tag="ACG86D_000618"
     CDS             35858..37210
                     /locus_tag="ACG86D_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFN3139793.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            37207..37635
                     /locus_tag="ACG86D_000619"
     CDS             37207..37635
                     /locus_tag="ACG86D_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFN3139794.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            37803..38168
                     /locus_tag="ACG86D_000620"
     CDS             37803..38168
                     /locus_tag="ACG86D_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3139795.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            38302..38874
                     /locus_tag="ACG86D_000621"
     CDS             38302..38874
                     /locus_tag="ACG86D_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN3139796.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            38889..39671
                     /locus_tag="ACG86D_000622"
     CDS             38889..39671
                     /locus_tag="ACG86D_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFN3139797.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            39671..40906
                     /locus_tag="ACG86D_000623"
     CDS             39671..40906
                     /locus_tag="ACG86D_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFN3139798.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            40980..41357
                     /locus_tag="ACG86D_000624"
     CDS             40980..41357
                     /locus_tag="ACG86D_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFN3139799.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            41359..41553
                     /locus_tag="ACG86D_000625"
     CDS             41359..41553
                     /locus_tag="ACG86D_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139800.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            41537..41713
                     /locus_tag="ACG86D_000626"
     CDS             41537..41713
                     /locus_tag="ACG86D_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFN3139801.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            41710..41853
                     /locus_tag="ACG86D_000627"
     CDS             41710..41853
                     /locus_tag="ACG86D_000627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139802.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            41936..43501
                     /locus_tag="ACG86D_000628"
     CDS             41936..43501
                     /locus_tag="ACG86D_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3139803.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            43557..>43984
                     /locus_tag="ACG86D_000629"
     CDS             43557..>43984
                     /locus_tag="ACG86D_000629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139804.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLF"
BASE COUNT        14146 a         7962 c         9892 g        11984 t
ORIGIN      
        1 gtttttgact gatttatatt gcgagtagaa aaaaaatatg ctattatttg tattgaggtg
       61 atcgtgaaaa tgcttacaga ggaacgtaga caaaaaatcc tagacttgct tgaacagcag
      121 aaaatcgtga aatcacaaga tcttgtaaac ctttttaatg cttctgaatc aacgataaga
      181 cgtgatttac aagagctgga gaatagtggg tttctagaaa gagttcatgg cggagctaag
      241 aaagagcaat tattgaattt tgagcagaat atgagtgaaa aatcactcaa aaacgttcac
      301 gaaaaacagc aaatcgccaa atcggcagct gaaaccatta atgacgaaga tgtcatttat
      361 ttagacgctg gctctactac gttagaaatg attccttttc tgcaaggaaa acagatcaca
      421 gtagtgacaa attccgttca acatgcggca aatttagttg atttggagct tgctaccatt
      481 attttaggtg gaacgataaa gctggcaact aatgctgttt taggatctaa tgcgatagaa
      541 caactaaaaa attttcattt taataaagca tttatgggaa tgaacggtgc ccatcttgaa
      601 caaggattca cgactccgga cccagaagag gcagctgtta aacgtttagc catgaaaaat
      661 tccgaagaga catttgtatt gattgatcat accaaattca accagttgtc cttcacctct
      721 gttgcacctt tggaagcagc cacgatcata accgagcgtt gtccattaga attcgttaat
      781 gaattccgtg aaaagacaac aattaaggag gcaatacaat gatttacaca gtaacactta
      841 atccatcaat tgattatatc gtacgcgtag atgggttaaa actaggcgat ttgaaccgaa
      901 tgaaggaaga cttcaagtta cctggaggaa aagggatcaa tgtttcccgt attttgaaac
      961 ggatcgattc tcaatctaca gcactagggt ttttaggagg gttcactggc gaatttattt
     1021 cgaagtggct tgaaaaagaa caaatccagt ctttgttcac aaccgtaaaa gaagatacaa
     1081 gaatcaatat caaactaaaa tcagatactg aaactgaaat taacggtttg ggcccatcta
     1141 tttcagatga agaaatcgat gagttgaagc aagcattagg aacagtagga caaggagata
     1201 tcgtcgtatt gtcaggaagc gcgccggcta gcctgcgaaa aggattttat caagaactga
     1261 ttgatatcat tcgagaaaaa ggtgcagatt tcgtgattga tacaacaggt gatgacctaa
     1321 aaaatgcact tgttaagaaa ccattgttga tcaaaccaaa caatcacgaa ttagctgagc
     1381 tttacaatac aaccttccaa tctgtagaag aaatcattcc gtatggtaaa aaattgctag
     1441 ctgaaggggc acagaatgtg ctgatctcaa tggctggaga cggtgctttg ctcttcacgg
     1501 aacaaggaac ttataaaacg aatgtattag ttcgcccatt gaaaaattca gtaggtgcag
     1561 gggattcaat gattgccggc tttattgggg cttacagccg tacgaaagat ccaatcgaag
     1621 cctttaaatg gggagttgct tgcggaagcg caacagcttt ttctgatgac ttggcagctc
     1681 gcgaattgat tgatgagttg ctgccagaag ttgaaatcac aaaaattgat tagtttcaag
     1741 gaggataatc atgaatatca aggatttaat ggtcaaagaa gcgatgatca tggatttgca
     1801 agcgactgat aaaaaaggcg caatcgatga aatggtccaa aaaatgtttg atgccggccg
     1861 gatttccgat atcgaaacat ataaagaagg aattctagcc cgcgaagcac aaacttctac
     1921 aggtcttggt gatggaatcg ccatgcctca cgcgaaaaac agtgcagtta aagaagcaac
     1981 agttttattc gcaaaaagcc aaaaaggagt agattatgaa gccttagacg gacagccaac
     2041 ttatctgttt ttcatgatcg cagctccaga aggcgcaaac gacacacatt tgcaagcatt
     2101 agcagcacta tctcgattgc tgattgatgc agatttcgta ggaaaattaa aagaagcaaa
     2161 tacaccagaa gaagtccaag acttgttcca aacagctgaa gagcaaaaag aagcagaaga
     2221 aaaagcagaa caagaaacac aagctgcaga agcttctgct catccagaaa gaaaattcgt
     2281 tgttgccgtt acagcttgtc caacagggat cgctcacact tacatggcag aagatgctct
     2341 gaagaaaaaa gcgaaagaaa tgggtgtgga gatcaaagtc gaaacgaacg gttcagaagg
     2401 aatcaaaaac cgattgactg ctgaagacat cgcccgtgca gaaggtgtaa tcgttgcagc
     2461 agacaaaaaa gtagagatga atcgctttga cggaaaagaa ctgatcaatc gtcctgtcag
     2521 tgatggtatc cgtaaaccag aagagctgat caatctagcc cttagtggta ctgcaccagt
     2581 attccatggt gatggaaaag aaagcagttc agaagaagga aatgcggatg gtactatcgg
     2641 acaaagaatt tacaaagatt tgatgaacgg cgtatcacac atgttacctt tcgttattgg
     2701 tggaggaatc gctattgcgc tttctttcat gatcgaccaa tttattggcg tgccacatga
     2761 tcaattatca aatctaggga attacaatca agctgctagc tggttcaacc aaatcggaca
     2821 agctgcattt ggtttcatgc tgccggtatt agcaggattt attgcttcaa gtatcggtga
     2881 ccgccctggt ttgatcgttg gatttgctgc tggtgcatta gctaatacag gcggtgcagg
     2941 attcttaggt gcactgattg gtggattctt agctggttat gtcatcgtat ttttacgtaa
     3001 attattcaag aacttaccaa aatcattgga tggaattaaa acgatcctct tttatccagt
     3061 gtttggctta ctgattactg gtttcttgat gttgctggtc aatgtcccta tgaaagcaat
     3121 caatgatgca ttgaacggtt tcttgacaag tatgagtggt tcgaatgctg ctttacttgg
     3181 tgcattatta gctggaatga tggcggcaga tttaggcggt ccaatcaaca aagccgctta
     3241 tgtgtttggt acagcaactt tagctacgac tgttgcaact ggcggaagtg tcgtgatggc
     3301 ttctgttatg gctggaggaa tggtacctcc attggcaatt ttcgttgcta cacgtttgtt
     3361 taagaataaa ttcagcaaaa cagatcaaga tgctggtttg acgaacatcg tgatgggact
     3421 atcgtttgtc acggaaggat cgattccatt tgcagcagct gacccgattc gtgccattcc
     3481 aagttttatt attggttctg ctttaactgg cggattagtc ggagctttcg ggatcaaatt
     3541 actggctcct catggcggga ttttcgttgt attcttactg agtcacccgg tcatgtattt
     3601 ggtgtttatt gctatcggag cagtcgtatc tggtgtgatt tatggtttat tgagaaaagc
     3661 tcctgcagca gcagaagcat agaaataaaa aagaagaggc tggtttttcc cagtctcttc
     3721 tttttttata gaatcatata gaatcaagta gtgtagatga atagttgttg taaaatagtt
     3781 tttccatgtt cgttaaaatg attgcttcaa atttgtgata gaagatttag cctcgatttc
     3841 ctgcttgatt cttggcaagt ttgcttcatc agtcaagata accaaaatat ctcctacatt
     3901 catgatcgta tcaccgtgag tcaagatttc ctgatttcct ctgcgtatag agattagcag
     3961 catattttta ggccaattga agtctcgtac cattaatccg tctaaagaac tctctgccgt
     4021 tactggaaaa tcaaaagttg tccttgcacc ttttagtgag gcggtctgag tatttccaag
     4081 tagacgttcc aataatgatt cgtaaattgg attaccgccg agaaggtcat tgacgatata
     4141 agcaacaaga gcagccaatc ctaaaggcat cagatgatta agagagccaa ccatttctgt
     4201 cactaaaata attgctgtca gcggtgcttt tccaatcgct gtgaaataac cagccatggc
     4261 aataacgaca aaatccttga tgtagattgg atccatcccg aaatcaacca taatgtttcc
     4321 ataaaagcta ccgattaaag cccccaatgt caatatcgga aggaaaatcc cccctggaag
     4381 accaccaccg taagaaatca tggaaaaaac gaatcgaaga acaaacaaac cgataatcat
     4441 aatgattggc aaggaagcat gtccaaaagc taaaacgatt tgattgcctc cgcctagagt
     4501 actaggccat aaataaccaa ttggcaaaat cagcaagaaa ggaacgaacc cgtgaaaata
     4561 agctggtaaa ggcaatcggt tgtaaaattt tggcaaagcc agcaagacct tctgataaat
     4621 aaaacctaag atgcctagaa gtactcctaa gatcactaag gaaccataat atttcaacgg
     4681 caaagaagga aggtcaccta gatataaaac aggtctcaag ccgaaaaagt aaagagagac
     4741 gaagttagca gtgattgctg aagcaaatga tgtcagccaa acaagtggag aaaaggagtg
     4801 atggatttct tctaaaacaa agagtaatcc tgcgatagga gcattgaatg ctgctgcaag
     4861 tccgctactt gcaccgctag aaattaaaat cttttcttct gaagatggac tccgaaacaa
     4921 ttgtccagct ccttgtccaa tggatgcgcc aagctgtata gaaggtcctt ctctacctag
     4981 aaaaaggccc gcaccaactg aaagtacacc gccaatgaac tttttccata aaacagagaa
     5041 ccagttcatt gataattccc cgcgaagctg tccctctact tgtggaatcc cgcttccctt
     5101 gatatccggt tcatttttga ccagataacc cacgaaaaat gctgcaagga tgcagcaaat
     5161 agctaggcca attaagtaaa tcggctgttc ccgactcatt tcatagatac ttcgaactaa
     5221 atcgcttagc ttttcgatag ataatcgaaa caggctaacg acgattcctg tcagcagtcc
     5281 aacaaatatt ccttttagaa taaaatatat ctgagtctta tctaatttct tgatttttcc
     5341 ttttttttcc attcaaacgc ctcgctttct tttaatttag cgcgaaatca acgaattgac
     5401 aaggaaaaat ccataaaaag aaacaaatga ttttgaacta gctagtttaa tgaaagccaa
     5461 gaaaatgcta taattaacaa gattgataaa agaaaggatg ttgttaatgt catccaaagc
     5521 tgaattatta aacgggatga acccccgtca gaaagaagct gttcttcaca ctgacggacc
     5581 attactgcta atggcaggtg caggaagtgg aaagacgaga gtgctgacac accgcattgc
     5641 ctatttgatt gaggaaaaag aagtcaatcc ttggaacatc ctagcgatta cttttaccaa
     5701 taaggctgca aaagaaatga aagaacgtgt caatgcgatt ctcgcatctg gaggcgaaga
     5761 tgtctgggta tcgacttttc actcgatgtg tgtgcgtatt ttaagaagag atgttgattt
     5821 tatcgggtat aaccggaact ttacaatcat cgattcttct gaacagctga ccttgatgaa
     5881 acggatacta aaagaactga acattgatcc gaaaaaatat gatcctcgaa gtatattagg
     5941 aacaatctct caagcaaaaa acagcctgca gacccctcag gattttgcaa aaatgcaagg
     6001 cagttattac gaagagattg cggcaaaatg ttatgctgct taccaaaaag agcttcaata
     6061 taatcaatgt atggactttg acgacctgat catgaatacg attcgattat ttgaagaaca
     6121 tcctgattca ctcacatatt atcaaaacaa attccattat attcatgtag atgagtacca
     6181 agatacaaat cacgcacagt atacgctagt caatttattg gcaggtagat ttaggaacct
     6241 gtgcgtcgtg ggagatgcag atcaaagtat ttacggctgg cgcggtgcag atatgcagaa
     6301 catcttggat tttgaaaaag attatccaga tgctgcagta attttgctag aacaaaacta
     6361 tcgctccacg aaaaacatcc ttagcgcagc aaaccaagtc attgaaaaca atagcaatcg
     6421 aaaaccaaaa aacttgtgga cagaaaacaa agaaggaaac aagatcactt actaccgtgc
     6481 agataatgaa cgagacgaaa cccgttttat cgtagatcgg atgcaagaag agattcgaag
     6541 caatcatcgg aattacggcg actttgccat cttgtaccgg acgaacgcac aatctcgtgt
     6601 aatggaagaa actctgttga aagcaaatat tccttataaa atggttggag ggcacaagtt
     6661 ctacgatcgt aaagaaatca aagatatttt agcgtatttg aatgtgctgg caaatcctca
     6721 agattcaatc agttttgagc gtatcgttaa ttcgcccaaa cgaggcatcg gaccaggatc
     6781 tatcgaaaaa ctacgcagtt ttgcttctct tcatgaatgg ccactgcttg aagcagcgca
     6841 aaatgtggat ctggcaaata tcggtggaaa agcaggacag caattaggtg catttggaga
     6901 gatgatccaa gaagtcactc agatgatccc atacttgaca gtgacagaat tgacgaaaga
     6961 ggttttggat cgtagcggat atctagaaga cttaaaaatc caaaatacat tagaagcaca
     7021 agcacgcatc gaaaacttgg aggaattttt aacggtcaca caagaattcg acaaacaatt
     7081 cgaacagcaa aatgaagaag acgctgatgc accagaagaa aaactaacgg tcttcttaaa
     7141 tgatctggca cttgtttccg atattgacaa tttagaagaa gatgcttccc aagtgacatt
     7201 gatgactttg catgcagcca aaggattaga attcccagtt gtattcctga ttggtctaga
     7261 agagggggtt tttcctttat ctcgagcatt gatggaagaa agcgaactgg aagaagaacg
     7321 ccgtctcgca tatgtgggga tcacgcgagc agaagaagcg ttgtatttaa caaatgcttt
     7381 ctccagaaca ttatatggac ggacgcaata taaccgacct tctcgttttg tagaagaaat
     7441 cgatcaggag ttactggaaa tcgaaggcat gcgtcctacg cctaaaaaaa cacctgtatt
     7501 cgctaaaaaa acagcatata gctataaaca gccagagaca gcggttgttc cctctaaaag
     7561 tgctactgga ggggaaaata acagctggaa acctggagat aaagtcaaac acaaaaaatg
     7621 gggtctagga acagtcgtcc gagtaagcgg gacgtcgaag gatctagaac tagatgtggc
     7681 tttcccaagt caaggagtaa aacgtctttt ggctgccttt gcgccaattg aaaaagctta
     7741 gtagtggagg gtaaatgatg gaacatgaaa tgacgattga cgaagctgct aaacgagcag
     7801 aagaacttcg tacaagactg aaccaatggt ctcgcgaata ctacgtagaa gataaaccaa
     7861 cagtggaaga ctatgtttat gataaagaat acgcagaatt agtagcaatt gaggaacaat
     7921 atcctgattt gatcacgtct gattctccca ctcaacgagt aggcggaaaa gtattagaag
     7981 gttttgaaaa agtcactcat gatattccgc tttacagttt gaacgacgtt ttcagcaaag
     8041 aagagctgat agcttttgat caacgtgtac aaaaagcagt gggacgtgta gttgactatt
     8101 gctgtgaact aaaaatagat ggactttccg tttctttgcg ttatgaagat ggaaatttcg
     8161 tgagaggtgc gactcgcggg gatggaactg taggagaaaa catcactgaa aacctaaaaa
     8221 ctgtccggtc ggtcccaatc aaattaaaag aaccaatgaa catcgaagta agaggcgaat
     8281 gtttcatgcc aaaacggtca tttgtccaat tgaaccaaga cagagaagca gagggaaaag
     8341 atatctttgc gaatcctcgg aacgccgcag ctggttcttt gcgtcagtta gattctaaaa
     8401 tcacagcgaa aagaaatctg gacacttttt tatacacagt agctgatttc ggtcctatgc
     8461 aagcaaaaac ccagtatgat gcgctagaag agctagaaaa aattggattc cacacgaatc
     8521 gggaaaaacg tctatgtcat tcgattgatg aagtgtggtc atacattgaa gaatatcatg
     8581 acaaacgtgt ggatttgccc tatgaaatcg atggaatcgt catcaaagtg aatgaattct
     8641 cgctacaaga tcaacttggt tttactgtca aagcaccgag atgggcagca gcttacaagt
     8701 ttccgccaga agaagttgaa acgctaatcg aaaacatcga atggacagtc ggtcgaacag
     8761 gggtagtaac gccaacagct atcatgacac cagtccgagt agcaggaacg acagtaagcc
     8821 gtgccagcct gcataacggt gattatatta agttgaagga tatccgcttg aaagatacag
     8881 ttctgattta taaagcggga gacatcatcc cagaagtttc gcaagtcgtg ttagataaac
     8941 gacctaaaga cagcgaagaa taccaattgc ctacacattg tccagtttgt ggaagtgaac
     9001 ttgtccattt ggatgaagaa gtagccttac ggtgcatcaa tcctaaatgc cctgctcaaa
     9061 tgaaagaagg tttgaaccac tttgtttcaa gaaatgcgat gaatatcgat gggttggggc
     9121 cgcgcgtact agaacagatg tatgataaaa aactagtagc agatgttgct gatctttaca
     9181 aattgacaga agaagagtta ttgacgttgg acaaaataaa agaaaaatca gcaaataata
     9241 ttttgacagc aatcgacaac agcaaagata attcggtcga gcggttgatt ttcgggttag
     9301 gaatccgaca tgtcggagcg aaagcagcaa aaattttagc agaacatttc ggtgatctag
     9361 aaacattgag taaaagtgac tatgagtcga ttattgcgct tgatacgatc ggtgatatca
     9421 ttgccgacag tgtagtcact tatttcagta acgaagaagt ccatgaattg atgaatgaat
     9481 taaaacaagc aggcgtcaat ttcgaataca aagggcttcg aaatgcccaa ttgcaagaag
     9541 tcgagtctcc ttttaaagaa aaaacagtag tccttactgg taaactaaca cgatttactc
     9601 gtgaagaagc aaaagagacg atcgaaaatc ttggcgggaa agtgactggt agcgtatcga
     9661 aaaaaacgga tatcgtggta gcaggagaag atgctggcag caagctgacc aaagcacaag
     9721 aattaggaat cgaagtttgg acagaagatc aaatggcaga tgcattggct aaaagtcgct
     9781 cagtagaaga atgaaaaatg agttttttca gacaaatttc aataaacaat ttcagacacg
     9841 gcactcttgt gttaaaatag agagcaaagt ttttaactcg tttagaaaga aggggtagta
     9901 tggcgattag tgaagaacaa gtgaaacacg ttgccaaatt agcaaaactt gcgttcgcag
     9961 atgaagaact tgcaagtttt accgaccaat taggtaaaat catcgatatg gttgaaatgc
    10021 tggaagaagt agatacagaa ggtgttccat ttacgtcgaa tgtggttgaa acagtaaacg
    10081 tgatgcgaca agatcgggcg caagctggat ggaaccgcga tgaattattg aaaaatgtgc
    10141 cggaaacaga agacggcttt atcaaagtac ccgcaatcat cgataatggg gaggctggcg
    10201 cataatgaca gaattacaca atcgttctct agaagaactg catcatatgc ttgtatcgaa
    10261 agaaatcact gtacaagatt tgacaaaagc aacatttgaa cgaatcaaag aaacagagcc
    10321 tgaggtcgaa gcattcatta cattgaacga agaaaaagca ttggaacagg caaaagcttt
    10381 agatgaaaaa gggattgatg aaagcaacgt ccttgctggt attccaattg gtatcaagga
    10441 taatatcgta acaaaagata tcttgacaac cgctgcatct aagatccttt acaattttga
    10501 accaatttat gatgcaacgg tcatggataa aatctatcaa gcagatatga tccctgttgg
    10561 taagctaaac atggatgaat ttgctatggg tggaagtaca gaaacttctt atttcaagaa
    10621 aacaaaaaat gcttgggacc caacaaaagt acctggtggc tcttctggtg gttctgcagc
    10681 agcagttgca gctggacaaa ttccagtttc tttaggtagt gatactgggg gaagtatccg
    10741 ccaaccagca gcttttaacg gcatcgtagg gatgaaacct acttatggcc gtgtctcacg
    10801 ttttggtttg attgcctttg cttcatctct tgaccaaatc ggaccaatga cccgtacggt
    10861 gaaagataat gcattagcat tgacagctat cagcggttat gacgaaaaag acggtacttc
    10921 ttcaggcgtt tcagttcctg attttgcaga gggactgaca ggcgacatca aaggaatgaa
    10981 gattgcttta cctaaagaat acttaggcga aggtgtggcc cctggcgtaa aagaagctgt
    11041 attgaaagca gcagaaacat tccgttcatt aggtgctgtt gtggaagaag tcagcttgcc
    11101 tcattcaaaa tacggtgtgg ctgtttatta tatcattgcc tcatcagaag caagttccaa
    11161 cttgcaacga ttcgatggta tccgctacgg ttatcgctca gaaaatgtga aatcattaga
    11221 tgatgtatat gtcaactcac gttcagaagg attcggtgaa gaagtaaaac gccgtatcat
    11281 gttaggtaca ttctccttaa gtgctggtta ttatgacgct tacttcaaaa aagcgggaca
    11341 agtccgtaca ttgatcaaac gagattttga aaaagtcttt gcagattatg acttgatcat
    11401 cgggccatct tcaccaacag ttgcattcgg aatcggtgag aacattaacg atccaatcac
    11461 aatgtacatg agtgacatct taacgatccc tgtcaacttg gctggacttc caggtatgtc
    11521 tgtaccggca ggtttatcag aaggattacc agttggattg cagatcatcg gtaattattt
    11581 tgatgaaaaa acgatgtatc aagcggcata tgcgtttgaa caagcaacag aattccataa
    11641 acaacaacca gctattttag gagggaaaga cgaatgaatt ttgaaacagt cattggacta
    11701 gaagtccacg tcgagttaaa aaccaactca aaaattttct ctccagcgcc agctcatttt
    11761 ggtgcagaac caaacagcaa tacaaacgtg atcgactggg gttatccagg cgtgctgcca
    11821 gtaatgaata aagctgcgct ggaattcggg atgaaagcag ctttggcatt aaactgtgaa
    11881 atctctaaag atactcattt tgaccggaaa aattactttt acccagataa tccaaaagca
    11941 tatcaaattt cccaatttga tcaaccaatc ggtcatgacg gatggatcga aatcgaagta
    12001 gaaggtaaaa agaaaaaaat ccggatcgaa cgtgtccatt tagaagaaga tgctggaaaa
    12061 aacatgcatg gtattggcgg ttattcttac gttgatttga accgacaagg cacgcctttg
    12121 atcgaaatcg tttctgaagc tgatatgcgt tcaccagaag aagcatacgc ttatctagaa
    12181 gcattgcgat caattatcca atttacagaa gtcagcgatg tgaaaatgga agaaggctct
    12241 atgcgctgtg atgccaatat ttctcttcgc ccttatggac aagaagagtt cggtacgaaa
    12301 gcagaattga aaaacttgaa ctctttatct ttcgtgaaaa aaggtttagc ttttgaagaa
    12361 aaacgtcaag caaaagttct tctttcagga ggagaaatcc aacaagaaac acgccgtttt
    12421 gacgaaacaa cgaataaaac agttttgatg cgtgtcaaag aagggtctag tgattatcgt
    12481 tacttcccag agcctgatat cccgaaattc gtgatcgatg acgaatggat tcaaaaagta
    12541 aaagctagtt tgcctgagat gccagcttct cgtcgcgaac gctatatccg tgagtttgga
    12601 cttccagagt atgacgcaat ggtattgaca cttacaaaag aaatgtctga tttttttgaa
    12661 gctgttttgg aaaatggtgc agatgccaag caagcatcta actggttgat gggtgaagtt
    12721 tctgcttact tgaacagtga gaaactggaa ttagctgaga caaaattgac acctgagaac
    12781 ttagcaggaa tgattcgttt gatcgccgat ggaacgatca gctcaaaaat cgccaaaaaa
    12841 gtcttccgtg aattgatcac aaatggcgga gaagcccaag aagtagttga aaaaaatggt
    12901 tgggtgcaat tatctgatcc gaacaaacta ctgccaatca ttaatgaaat tttggataac
    12961 aaccaacaat ctgtcgatga tttcaaaaat ggaaaagatc gtgcggttgg cttcctagtc
    13021 ggtcaaatca tgaaagcaac taaaggacaa gcaaatcctg gtgtcgtgaa taagctactt
    13081 aaagatgaat tagcgaaacg atagaggctg aaaatgaaaa aagcacgcgt aatttataac
    13141 cccacctcgg gaaaagaatt attgaaaaaa aatttggcgg atatcctaca ggcattagaa
    13201 gaagcaggat ttgaagccag cgcctatgca actacaccag aaccagattc agccaagaat
    13261 gaagcatgta gagtcgcaga actaggtttt gatctagtag tggcagctgg tggcgatggc
    13321 acaatcaacg aagtggttaa tggaatcgct ccgcttgaac accgtcctaa aatggccatc
    13381 atcccagctg gtacaacgaa cgactatgct cgagcattaa aaattcctag agataatatt
    13441 aaagcagcag cagaagtgat aaaaaaacac caaaccgtaa aaatggacat cggcctttct
    13501 gataacagtt acttcatcaa tatcgctgca ggtggctatt tgacagaatt gacttatgaa
    13561 gttccatcag aactaaagag tatttttggt tatcttgctt atcttgcgaa aggagcagag
    13621 atgctccctc gagtaaagcc aatcaaaatg cgcttgaagt atgatgaagg ggaatatatc
    13681 ggaaatgctt cgatgttttt cctagggctg acgaactctg ttggaggatt cgaacagatc
    13741 gcgccagatg ccaaattgga tgatgggaag ttttctttaa tcattgtgaa gacagcaaat
    13801 atctttgaga tcttgcatat tgcagcactc atgctgaatg gcggtcgaca tgtcgatgat
    13861 ccaagaataa tctataccaa aacaagcagt ttgttcgttg aaacagaaga tgatatcaat
    13921 cgaccagtaa tgatcaactt agatggtgaa tacggcgggg aagcaccgat gcatttccaa
    13981 aatcttcatc aacacattga gttctttgcc aataccgatc agattcctga tgaagcaatc
    14041 acaggtacag atgaagaaga attagaggtt gtcagcaaag aattcgtaaa agaagtcgaa
    14101 cgtttaacgg atgaagatat cgacggtgac ggaaaaatag cagacgaaac acctaatcaa
    14161 aagaattaat caaaagccgg tgctgaacgt agtaaggtca gtgccggttt tagttgtgta
    14221 attagctgat ttatagtaga gttcagtagg attgaaaaat aggcttaaag gagcaaaata
    14281 tgacagaaca gcaaatagta aagaaaaacg aacgatatac agtcgaaatc gtcgacttga
    14341 gttatgaagg aatgggggta gcaaaaatcg atggttatcc attatttatc gaaaatgcac
    14401 tccctggaga agaagtagaa atactagtag taaaagcagg caataagttt ggatatggaa
    14461 aagtcgaaac atttattaca caatcacctg atcgtcagcc tgtagaaaac aatgcattac
    14521 ttcgcatagg tatcgcacct ttagcccatt tgaagtatga acaacagctt gtcttcaaac
    14581 aaaaacaagt agagcatgta atggcgaaga ttgctaagat gccagaagtt cctgtacttc
    14641 caacggtcgg aatggcagaa ccattcggct accgtaataa agcacagatc cctgttcgcc
    14701 gtatcgatgg acagttagcc accggttttt acaaaaagaa tagccatgat ctagtcccta
    14761 tcgaagattt ttatatccaa gatcctgtca tcgatcaagc aatcaaagta gtcagagata
    14821 tcttgcaacg gttccaagta cgaggatata atgaagaaaa aaatgaagga cagcttcgtc
    14881 acatcattat ccgtcgtggt cattattccc atgaaatgat ggtcgtttta gttactcgta
    14941 aagaaaaatt ctttaaatcc aaggaaattg ctgaagctat tcatgaagaa ttgccagaag
    15001 tggtttcggt catccaaaat atcaatgaag aaaaaacaaa tgtgatttta ggagacaaag
    15061 aaaaagtttt atatggcaga gactatatcg aagatcagct attaggaaaa acctatcgaa
    15121 tctcagcaaa gtcattttat caagtaaata ctcaacaagc agagtatctg taccaaacag
    15181 ccatcgagtt tgccgagtta gataaaaaag acattgtgat cgacgcttat tcaggaatag
    15241 gtacgattgg cttgtcttta gcagacagag tggataaggt gtatgggatg gaagttattc
    15301 cagaagcaat tgaagatgca cagttcaatg ctttaaataa taaaatcgaa aatgttcatt
    15361 atgaagtcgg aaaagcagaa aaagttatga aaaaatggca agaaaaaggg atcaaaccat
    15421 ctgtgatcgt agttgaccct ccacgaaaag gattagatca tcattttatt gaatcagcaa
    15481 tcgaaatgtc accagaacga atcgtctata tctcatgtaa tccggcaact tttgcaagag
    15541 atgctaagtt atttgcagaa gcaggttatg ccgtgaagaa agttcagcct gtagatcttt
    15601 tcccgcaaac ccatcacatc gagtgtgtag gactgcttac acgtgtggaa cgttgatatg
    15661 acaaggatta gagcgattcg ggatggtttc cggattgctc ttttttcgtg ttgaaatgac
    15721 gatttttggg tgagcgtaga gttgatggaa atgctatgga gctttgagtt gataggagtt
    15781 acaccgtaga cttgataggt tagatgattt tgataacggt tacgtgatat aatatattat
    15841 cgagttttca agggagacaa acttatgaag caaattttta tattttcaga tacacagaaa
    15901 gataatattt cttattttga acgcgagcca gcaatcttgg tttcaatgag tcgtagtgat
    15961 ttgccgtatt tgaaggaaca ggatttttca aattatccag ctgtttatgt tttgattggt
    16021 ggtaataagc gttacgtggg tcaggcagca gggcaatcaa tttcacttcg tttatctcaa
    16081 cactttctca aggaatataa agcttgggtt gaatcagttt tattctttac aagagcagat
    16141 gggaaaatgt caaaagcaga tacagattat cttgaaaaac gacttatcca agatttccaa
    16201 gaaaaatctg actatgaaat gatgaattca actgctggta atacttcgta tattgataaa
    16261 cttcaaaaag caaaatcgga tcagctatat gacacagtgt ttgaaattat agatgaaatt
    16321 gcaaatatcg atttgtttgg tacttccgaa gacagtgaaa cccaaattta tgaatttagt
    16381 tcatctggac aatatgaaat tgagtatgat gggaaaaaga tatcttcgaa atctgctaga
    16441 ggtttattgg ttgattttgt ttctcagcta tttgcggatg gaaactacaa caatcaactg
    16501 caagaattaa ttgtcgatga gacgccgtcg ttcgctataa ttttaggacg taaaccatcc
    16561 atgtataacg gaagaccaaa ttcagccagg gtagctgatg gagtatgggt ttatgcaaat
    16621 ttcaacaaaa agaatgtcag aatcaaaatt gataaactag ctcgtcaatt ggggattccg
    16681 gttaaaataa agtggaatta atatttgtta tctcgccttt agtaaatcta tgctgaaggc
    16741 ttttatttta gaaaaatagt ttgcgttcgc gaaaatattt catgtttcgt ataataatga
    16801 tagctcatag atgatataat tgaagatacc agtagaatag aggtgtagta tgtcaatttc
    16861 ttataataaa ttgtggaagt tattgattga tttgaatatg aacaaaactc aacttcgtga
    16921 aatatctggc gtcagtccca atgtaatagc aaaactcggt aaaaatgagt tggttgcaat
    16981 ggagtcatta ttgaaaattg ctgctgcctt aaatgttgat gttagtgaaa taattagttc
    17041 agatgttata gatgcggagg atgttaaata aatggcaaaa aataaagttt taaacatcac
    17101 tgaaattgaa gatggtgtaa aaaaaatact ttcaaatagg ggcgatgatt tcatcactca
    17161 atttttgtcc ttgtacgata tccccaaaac gtcgattaca cgtgcaaaat caaaattcga
    17221 taatggtgaa ccatttgtga ttaagaacaa acttcattat gaagaaattg aaggcaacgt
    17281 tgtcattgcg attgatgcga ttgctcaatc aatcaaagag caaaaatcta aaccacgata
    17341 tatcttggta aacgattatt tagatattgc tgcaattgat acgaaaactc atgaaacttt
    17401 gaatattcca attgaagagc taccggctaa tgctgacttt ttcttagcgt ggaatggcat
    17461 tgagaaggct gattatcaag ctgaaaatcc agctgaccga aaagctgctg aacgttttgc
    17521 aaaattgtat gatgtggtgg ctaaagataa tcctgatgcc aacgagcatg catttaactt
    17581 gttcttgatt cgagtgctgt tcttgttgtt tgcagaagat acaggaatta tgaataaggg
    17641 ttcctttaca aatgttctaa aaacaagaac ggctgaagat ggttcgaatt ttaacgaagt
    17701 aatcaaagaa ctctttgcaa tcttggatat taatgagtta gctcgcaatg gcaaggatga
    17761 gtggctactt gaatttccat atgttaatgg aaaactgttt ggtgagccac atgtggattt
    17821 gaccttttca aaagtttcaa gagagctgct tattgaagca ggggaattat tgaattggaa
    17881 tgaaatcaat cctgatatcc ttggcgcaat gattcaatca gttgcttcag cagaagaccg
    17941 tcacgttgcc ggtatgcatt atacaagcgt gccgaatatc atgaaagtca tcaagccgtt
    18001 attcttagat gatttgacag agtcatttga aactttaaag cttcgcagtg aagaaaacga
    18061 gctgaaggac attactgaaa aaacaagaaa cgataataag agaacaatca tcaatgatct
    18121 aagtgctttg ttgacgcgaa tttctaatat taagtttttg gatcccgcca gtggttctgg
    18181 taattttctg atcattgctt ataaggaaat ccgtagactt gaaattaaga ttttgcaatt
    18241 gttgggcgac ttacaaaata atgattccat gccactatca gtgattcatc ttggtaattt
    18301 taatgggatt gaattggatg attttgcgca tgaagtagca agactttcat tatggattgc
    18361 tgaacatcaa atgaataaag agcttgaaga agcaatacca ggtaccattg ctgagttgtt
    18421 accgttaaaa gatgctggga atatagtgtg tgccaattct cttcgaattg attggaatga
    18481 ggtgttgcga ctcaaagaaa atcatgaaat atatataatg ggaaatccac cgtatctggg
    18541 caaaagtctt caaaatgctg aacaacgtga agatatggaa aaacttgttg gcgatattcg
    18601 aaaatataaa ttgcttgatt atatttttgg gtggttttgg aaagctaaac aatatattgc
    18661 aacatctcat ggctcgttcg catttgttac tactaattca atttgtcaag gtgaacaagt
    18721 tagtatcctt tggccgtacc ttttaaaaga tgaggtaaga atttcatttg catatcagag
    18781 ctttaagtgg aacaacaatg caaagaataa tgccggtgtg actgttgtca ttattggtat
    18841 gacaaataat gtgaatattc atcctaagtt atatttagag aatgggtcag taatgagtgt
    18901 ttcaaatatt aatgcatatt tagctaatgg tgaaaatgta tttgtcaacg aatcaagaaa
    18961 tagtatttca aatttaccct ctttgaaatt tggcagtatg gcaaatgatg gtggtaatct
    19021 agttttttca gtggcagaat actggaattt gattcaaaaa tatccagata ttaaacattt
    19081 atttaagaaa tatttaggat caacaggatt aataaatgga gacttaagat atactttatg
    19141 gttgaccgag gaagatgcaa ctagatacaa acaaaattct cttattaaag aaacaataaa
    19201 aaatgtaaaa agttataggg aaaaaagcaa gagaaaagaa actcgagcac tagctgagaa
    19261 gccatggtca tttggtgaag ttcgttatga tgaaaccagt gagaatagta ttctaattcc
    19321 tcgtgtgtca tcagaaaaaa gagattatgt tcctatgggt atagttggga gcgatactat
    19381 aatttcagat tcggctatgg caatctataa cgctccaatg tggctgcttg gtctgttaga
    19441 atcccgaatg catatggtgt ggctgcgctc tgtcggtggg aaattagaaa ctcggtaccg
    19501 ttattcagcc gggttagtct ataatacttt tccgattcag gatttatccg cccagcgtaa
    19561 aaatgaaatg actcgcgtga tgacagaaat tcttgattta cgagaatacg aaggcggcac
    19621 gttagccgat ttgtataata aagacagtat gccagaatca ttacgtaaga aacaccagga
    19681 actggatggg attgttgata gagcttatca gcaacgcccg tttgaatctg acgaagatcg
    19741 acttggaaca ttactaaaac tctatcagga gatgacaatc aatgactagt aatttatttg
    19801 aaattaattt tgataaaaat caatcaaaaa caacaaatga cctcggtatg cgtgaaatgc
    19861 aagaacgtgt ttatgaaaag cgtgcgtcac agtatttgtt ggtaaaatca ccaccagctt
    19921 caggaaaatc acgtgcattg atgtttgttg gcttggataa actccataat caaggaatta
    19981 aaaaagtggt tattgcagtt cctgaacgct caattggtaa atcatttaaa gatactgaat
    20041 tatcgaagtt tggtttttat tgggattggg aagtaaagcc aagtgacaac ttaactattg
    20101 ggggcggtga aaaatcaaag gttcaacact ttgttaaatt catgaactcg actgacccaa
    20161 gtgataatat cctgattgcc acacatgcta cgttgcgatt tgcttttgaa gagttggacg
    20221 attcattatt tgacaatacg ctattagcga ttgatgagtt ccatcatgtg agccgtgatg
    20281 ataaatctgt gttaggtaat gccttacgta gcatcatgtc aaactctaat gcgcatatct
    20341 tagcaatgac tggttcatat ttccgtggcg actctgttca gattctggaa ccagccgacg
    20401 aagataaatt tgataaggtc acttacactt attatgagca actggaagga tataaatatc
    20461 tgaaatcctt tgcaatgggg tatagtttct atcgtggaaa atatacagat gctttggata
    20521 aagtaattga tgtcaacaaa aaatcaatta tccatattcc taatgttaat tctggtgagt
    20581 ctactaaaga taaatacgcg gaagttgatg aaatacttga tttattgacg gacggtggta
    20641 atatcacaca gaacgaggat ggtatttatg aaatcgaacg tccagatggt aaaaaactat
    20701 tagtggccga ccttgtgaat gaagaaggac gtgaaaaagt ctcagcatat ctagcaagta
    20761 ttacggatga cattaaagat cttgataagc ttgatatcat cattgcactt ggaatggcaa
    20821 aagaaggctt cgattggcca tttgcggaat atgcactgac aatcggttat cgtaactcat
    20881 taactgaagt gattcaaatc attggtcgcg taactcgtga cagccccaat aagactcatg
    20941 cacagtttac aaatcttatt gcgcagccag atgcacagga tgacgaagta ttattcgcgg
    21001 ttaacagtat tatgaaggca atttctgcat ctctacttat ggaacaagtg ttggcgccag
    21061 tttacaagtt taaaccaaaa gatggtaagc cagacaaacc aggtgatctt tcaatcagag
    21121 gactgaaacc tgctgatacg acggaacgta caaagaaaat catcgaaaat gatatggaag
    21181 atttgaaatc tagcatttta cagagccaag atattcaaaa tgcgattgtt tcaggtgctg
    21241 atgcgaagat gattaataag acgttgattc cacgtgtcat cattgatcgt tatcctgatt
    21301 tgacgaacga agagttagaa accgttcgtc aacatacagt tgccgatata aatattgcgg
    21361 ctggacggac aacaacttca gaagatggta gccgcgaaat tattaagatg gccgatcgtt
    21421 tcattaacat tgatgaattg aatattgatt taatcgatca agtgaatccg ttccaacgtg
    21481 cgtatgaagt gattagtcgt gatattgatg caccgactct tcggtttatt caggattatt
    21541 tgtcatcaaa taagtatgat tttactgacg aacaattggt tgcggcctac caagctgcta
    21601 agaagttccg tgcagaaaat ggtcgaaatc ctgatcgaaa tagtaaagat gatggtgaaa
    21661 aatacttggc ctttgctttg ctacgacttg ctgagataaa acgtgaaaga gaggcagcga
    21721 aagaagatgg cgaatagttt agatagcatc tttgaagatg acgcttttga tgatttaacc
    21781 aagcaagttg aaaaaaagaa agttgtaaaa caagacccag aagttgctaa atttcaggag
    21841 attatagact ttgtgaatga aaacggacga gagccagaaa aaactcctga atggtctagt
    21901 gaacgcgcat tgtgggctcg tcttagcgga tttcgaggaa aacccgaaag gtctgagaag
    21961 gttaagaagt atgacactct tggtttattg gagaaagaat tcgaaccgga tggagagttt
    22021 ctcgctgatt cagttgaaat cgatgacgag ttgaatttag atgatatctt aggctcagat
    22081 gatatgattg atgatttttc agacttgttg gatgtttccc gctatcgtag aactgtgaac
    22141 gctgcagata aaattggccg cagaaaacat gcaagtcatt ttgaaaagta taagagcttg
    22201 ttcgatcaag tgcatgcaga tatcgcatct ggcagacgaa aaattatccc gtttgagcag
    22261 tatgatattc atgaaaatag attctacatc caaaacggcg taatgatgta tatcgtttcc
    22321 atttcgaagg atacctttat cgctgataat gggaaaacaa atgccagaat gcatgttgtt
    22381 tatgagaatg ggacagagaa taagaatctg ttgttacaat ctttagcttc gtctctatac
    22441 tcgactgaac gtcatggaag gatggtgaca gagccggtag atgaggaaac actggctgaa
    22501 agctttggag aagagtttac gacaggttat atctacgtgc tgaagtcatt aagcacgaat
    22561 ccggatatta gcaagtggaa taacctgtat aagattggtt tcactcgaaa tgatgttgaa
    22621 agtagaattg ccaatgcgga aaacgacgtg acctacttaa atgcaccagt gaaggtcatg
    22681 ctttctgctg aagtacaaaa tgtcaacgct cagctacttg agcgcactct ccatcatgta
    22741 ttccaggaca aacaagttgt ctttcaggat ggaaacttca aaaaagctac tgaatggtat
    22801 atggttccat tgaatgatat tgaatcgaaa atcaatgaag taatcgctgg attacaaaaa
    22861 taaatatgaa aatgcactgt actcatgaat cgaatacagt gcatttttga tatttaatcg
    22921 cgaacgattt caacaatatc cgataaatca caatcaagtg cttcacagat gcgaagtaag
    22981 atatctgttg ttacattttc gcctttattt agttttgaga gtgttgctga actgatatca
    23041 gtcatttttc tgagttgtgc tttattcagt cctctgtcga ttaggagttt ccaaagcgga
    23101 ttgtagctaa acttcatgta atcattcctg ttctagttat aaatatcgta tagatatcca
    23161 tagcgtccat tgtttttatc ttcagtaaag acaatctgat ttacttgttt atcatcatag
    23221 tttagtgctg gcaagttcgt tgtattctca acgatgataa gttgtccttc gttttgatga
    23281 tccataaagt atttgaaaag tccttctttc atattgtcat cgccatcttg ttttttgcgt
    23341 tcatctaatc caagaagagg ggaatcgata atgaacacag aaggtgtgta cttagcgtga
    23401 ttatacaaga gatgtcgata tgccattacc actactgaat tgataaacgc tgtatatcca
    23461 tgtccgtttt cggtccattt tagatttcca ttgattttga tgtcgaaaat atccttggcg
    23521 aaaaatgcgt tacgaaattc attaggttta taatgcatat tacgtaacat ggagtccagt
    23581 aactcagaca tttctttgta gaattctgca tcaaatttct ctttaggttt ataaaggata
    23641 cgttcgtctt ctggctcatt ctcgattttt tcaagatcac ttttaagctt attttgcatt
    23701 ttttccatcg agattagctg ttgtttaata tccctgtaag tttcgatttt attgattcga
    23761 tcctttaacg tctcagcttt cggttttagt tcatcgttga ttgctgcatc aagcgatttc
    23821 tttcttgttt taagctcatc aagctgttcc attaactctt gcagttcaac ttcggtatca
    23881 tgagattctt ccgtcaaatc tgataattga gagagcaatg aatgtaattc tgacctagca
    23941 gtatcgatgt aggttgtttg gacaggttta cttagttcgc cgttacagaa tggacagcta
    24001 tgtgaagtat catgttcatt atgatcattt aataatcgct caccatctac gattagtgtt
    24061 agtcgttcaa tatccgattg atattgataa tgtaactgtt tgtacctatt caaaagaagt
    24121 tgagtttcat gaagatactg ttccttttga taaatctctt ttatcaagat tttgctgctg
    24181 gctaacgcat cattgatttg cttttcaatt gtttcaagtt cagcgaacat tccatcgaca
    24241 atttgttgaa aatcatcaga gggagcctca ggtaattctg atttagccac ttcgtagttt
    24301 tcatataatt cagaaaggct tgaattgatg tagttaatca ccgagttcct tctaattttt
    24361 ttatgttctt ttgtttcgtt ttcttccgag ttcgcaaaat ctgaaccgta tagcagaagt
    24421 aacaacgatg ataaaaaagc tgtgcgttgg gttgtttccc caggaaaaag aacagaggcg
    24481 ctggtattga ctctatcttg tggaataagt gtgctaggcg ctaatgtccg ccaagttaat
    24541 ttttgacgtt tgaaatcctc attcttgata atctgataat catcatcgat gcctaaaagt
    24601 tgtaaccaaa aatggctaat tggatttttc ccagatcgtg cattatattt ttcataatct
    24661 acatattcac ctgaactcgt tattttggat accaaaatgg agttgctatc aaattctcta
    24721 gttaatgaat atttgccatt agtcgattga ataatcagtt caactttact gtatccggtt
    24781 gcatcggttg aaaagggagc agcgtctttt ttcgcgccat agagaaaatc gattgccgta
    24841 acaatgctag tttttccggt atttgaaggg ccagaaatta tgtttagtcc attggtgaac
    24901 ttgacagttg catcttcacg atctttgcct tttatggtaa cacgttcaat ataataattt
    24961 tgcattatca ttgctctcct ttaaattcct ttgcgcgttg gtttatttgt cgcagaagct
    25021 caacgtcact tttatcttga atgtatttaa cagattcgtc agctatttgt aagtattttt
    25081 ctgcatatac cgatacgaat tgtgatatca actttaggcc atcttctgat aaagcatact
    25141 taaaaccttc tttgctttgc gatggttgga taacatgttg cagtacaaga tttttgactg
    25201 catctttaac taattcacgt cgcgcaataa attcactgaa tctatagctg ttatcaccat
    25261 ttaagttgta atcggaaatt tcaaagtcgc gaccatacgt ggtgatgaag tctgtgatta
    25321 aaattctatc agcgctgaca ggctctttaa attcagagag cagtaacatt actcgaaccg
    25381 aaatttcaaa tggagtatta aacaaactat tcatcttcag tcacccattt gatctttccg
    25441 tcattaacca gcatgtggca aatgcctttt ttagcagaat tttgcagcaa tcccggtata
    25501 aacgataaga ttgatccgtt taatgttata acagacgact gctgtagaac ggctttgagt
    25561 ctctcaacgc catcatcgta gtcttcttca tagacttcct cgatgccgtc gtacatatct
    25621 tcttttaaag cttcaaagtg ctcagttcct tcctcttgtg taaagttatc acggacccca
    25681 cgacgtatgc tttcagcttt atagtaattt cttcgctgtc tcttgaaatc ctttagccgc
    25741 gaatttggca ggtcgtttgt atgctcgtat acttcgtatc catcggcatc tgcatatgct
    25801 gaaaaaagag ctatgacata cgtcatctcc tcagctctaa tatcaagggg aactggcaaa
    25861 tcgtcaaaga attttttcac ttcatcatta taagagaaat caccattagt gttgattctt
    25921 aaacttgcga gctctaattc gctaagcttc ggcttggcct tttttgtttt tacttttgtt
    25981 tgagaagcaa ttgaggtgat ttcatcaagt aaaagatggg ccactatttc agaaacgtct
    26041 gtatctttgg ggattaccac acctttatca atcaattgtt gcttaaggac ggttggagca
    26101 tcatctataa gacgttcata aataaaagtc gtgcatttgt cgatgttgaa ttttccagca
    26161 acccgaactg catactcttg tgttaatggt cgagtaccat tgtaaaatct tcgtaggtta
    26221 tcatcagaaa aaccgaaaat ggtggcctcg tcatcatttg aaacactatc cagtaaagca
    26281 attaaaaaat cctgtggctt cacgtactcg cttcccataa acggataaag cgtttgagta
    26341 atttcactta tttccaaaat acaacctcct ttgttacgtc cgccgttgac cgccgtttgc
    26401 cgccgatgac tgacgaaaac aagggaggcc tttttctata attaaaacat agtagtttaa
    26461 ctatttaatt ttatcattta tggttcataa acactacatt atgtttcaag aaagtgaacg
    26521 aatttatctt aaaagcgaat agtcctacta ataaaatata acaatgcctg agttgcaata
    26581 gggcaaggtt acaaatactg aaaagaactg gttatccaga aaatttttca gatattgtat
    26641 gccttacttt attgcgctct tttttcgtga tgagggacgg tgtacgtatt ggcacggtcc
    26701 ctgtttgttt tccttgctca aaagctcttt caggcaagaa agagggatct aatgagtaga
    26761 tttatgaaaa cacaccaaaa cgaccgcagc acttacaagt acgtgtttga gcgtactgat
    26821 gccaatggac gtatccaaaa agaagtggtt gagattcgcc caggcgaaga cggcgtcact
    26881 gagcttaaca ttcaggagct gcatcgcgct gacgaccgtg cgatttacaa cttttataaa
    26941 aacgcacgtc ctgaacgcac agaagaagaa aaagaaaaga tcaaggcttg gaaagagaag
    27001 ttccgtgaag actttaataa tgagcacggc tattatccaa tggacgaata cgtggatggt
    27061 gaagccaacg attacttcaa acgtaactac aatttgtcgc tcaatgaatt tgatgatgaa
    27121 tgcgatgagg gggacaaccc catccaatca ttggtttacg atgcttacca agataatgaa
    27181 gaagatcctc gtatcgatcg tttgcgtgaa gtcgtgacgc aattattacc gtaccaccaa
    27241 gaaatctatc acatgctcgt cactgaaaac atgtctcaga ctgaagtggc ggacaagctt
    27301 gacaagaaaa aacagtcaat caatcgcacc tttggaatca tcaaagacaa gatcaaagaa
    27361 ttattttaga aaaaagttac gggggagttt actttccccg ttttccttcg cctgtattgt
    27421 gtaagggagt agatcctaaa caaataaaga tgaaggagga ccatcatgaa cctaaaacac
    27481 aaaatccgag taaacgtgac tgatgacacg ggtaacaaag aaactgtcct acaaggtggt
    27541 gtgcgtcatt tgcctcgctg gtttgcacgg tggctgttcg gtaagggcaa catggacaaa
    27601 attctcgtgc tcacaccagg ggacacaatc gaatcagtcg aaatccgtga agttaacaag
    27661 aagggagcgt aatgacgtga gtaaaacaaa actatttctt caggtcgttg aggacgtcca
    27721 agcgttagct gatagcttac aagagttggc tgtggcgatg aaaaccaatg aacctgaacc
    27781 acaagttgaa acaccagaac cgacaatgga aacaaaatca gaagtcaaac aaccgacgct
    27841 tgaagaagtc cgtggtttgt tggcacgtaa atctcaagaa ggtaagtcaa ctgaagtgaa
    27901 agcactgatt gagaaatatg gtgccagtcg cttgagtgat gtgccgacag aaaactacgc
    27961 ggctttggtt gcagatgcgg agggattgta gatgccaaca caacacgcag tcctatccgc
    28021 atcttcaagt aatcggtgga ttcactgtcc gccaagtgtt cgcttgtcag agcactttga
    28081 aaacaagtcg agcccgtatg cccaacaagg cactgatgct cataccttat gtgagtacaa
    28141 actgaataag ttcattggta atgacgtcac agacccaaca tctacgcttg aattctacga
    28201 cgaggagatg gagcagtgtg ctgaaagtta tgcgaccttc gtcatggaag aagtcgctaa
    28261 ggcgaaacag acaacagttg actctattgt aatagtagaa cagcggctcg acttctcaag
    28321 gtacgtccca gacggctttg ggaccggcga ctgtctcgtc attgcggatg gaaccctatc
    28381 tgttattgac atgaaatacg gtctaggaat tttggtagat gcgtatcaga acccacagat
    28441 gatgtgttat gccttgggag cgttggagct tttcgatgtt atttatgaca ttcaagaagt
    28501 caagatgacc attttccagc caaggcgtga gaatgtgagt acgtacacac tgctgaaaga
    28561 agaattactt cagtgggcaa aagatgtgtt gtcgcctgcc gcagatttag ctttcaaagg
    28621 tgagggtgag taccaatgcg ggaaatggtg tggattctgt ccggcgaaga atagctgtcg
    28681 tgcacgagca gaccataact tgaaactggc gcaatatgag ttcaaaccac cagagcttct
    28741 tagtgatgat gaaatcgaag aaatcatcgg taaagtggat gacctggttt cttggagtaa
    28801 cgatatcaag gactgtgctt tgaaacttgc actcggtggc aaacaatgga ctcatcacaa
    28861 attagtcgag ggtcgctcta ctcgtaagta ttctaatgac aatgatgtcg ctgcggcggt
    28921 catcaaggct ggatacgacc catatgataa aaagttactt ggtgtcacag caatgacgaa
    28981 ggcactcggt aaagagaaat tcaatgaaat cttgggcgaa tacatcatca aaccaaaagg
    29041 caagctgacg ctagtcgatg gttcagataa acgacaagcg gtcacagtaa acaacttaaa
    29101 cgaagaattt aaaccattaa cggaggaaca ataatatgtc taaaacaagc aaagtaatca
    29161 ctggtacaaa cacacgtctc tcatacttcc acggttggga accagcatcc atcaacgggg
    29221 gcgcagagaa atattcaaca gcagtcttga ttccaaaatc agataagaga acgattgata
    29281 ccattgaaaa agcaatcgat gcagcaatcg aagagggact tgcgaaattc ggtgggaaga
    29341 aaccaaacaa agcagaaatc aagctgccat tacgtgatgg ggatatcgaa cgcgacgatg
    29401 aagcatacca aggccattac ttcattaatg cgaactcaac cactgcacca caaatcgtgg
    29461 atcagcacgt tcaaccaatc ttggatcgtt cacaagtgta tagtggttgc tacgctcgtg
    29521 tgtcgattaa tttctatgcc tttaacgcaa atggaaatcg cggggtagcg gcagggcttg
    29581 ggaatatcca gtttgtacgt gacggcgaac cactcggctc acaagtatct gcaagcgatg
    29641 agtttgaaac cttggcggat gatgatttct tggcttaatc attaaaacaa aaacggccat
    29701 ggcagaagtg ctgtcgcctg tttgattgga ggaagaaaat gaaacatttg tcattggatt
    29761 tggaaacgta tagtagtgtc gatttatcga aatgtggtgt ctacaaatat tcagagtccc
    29821 ccgattttga aatactgcta tttgcataca gtgtagatgg tggtcccgtc gaagtggttg
    29881 atctgaagat ggacgaaact atcccggctg aaatattaac ttcgctatca gatgaaacag
    29941 tgattaaaca cgccttcaat gcgagttttg aacgtgtgtg tttgtcacgc tttctggggt
    30001 tggatggctt tttgaatcca cgagggtggc aatgcacaat ggtgtggggt gcgacattag
    30061 gtttgccgtt gtcacttgta ggagttggtg ctgttcttgg tttggataaa caaaagctca
    30121 cagaaggaaa agacctcatc cgatactttt gcgtgccatg caatccgacc aaagttaatg
    30181 gtggacgcac gaggaactta ccacaccatg cgccaactaa atggtcagca tttaagacat
    30241 acaatagtcg tgatgtggaa gttgaaatag ctatccaagg aaaactttcc aattttccag
    30301 tgcttgattc tgtttggaaa gaatactggc tggatcagga aatcaacgac cgaggaattg
    30361 agattgataa ggactttgtg gaagctgccg ttcagttgga tgaattatca aaagctggat
    30421 tgaatcaaca attgcaagaa ttaactggac tagaaaatcc aaacagcgtg cttcaaatgc
    30481 ggggatggtt actagaccac ggacttgaaa ccgagtcact cggtaaaaag gcagtggccg
    30541 aactactcga acaacctatt gatgaaaatt taaagcaagt cctggtctta cgtcagcaac
    30601 tcgccaagtc atccgtgaaa aagtatcaag ccatgcaaaa ctttgcgtgt tctgataatc
    30661 gttctcgtgg tttattccaa ttctatggag ccaaccgtac cggacgcttt gcaggacgtg
    30721 gcatccagct tcaaaacttg cctcagaacc atatgtcaga tttatctgaa gcgcgtagtt
    30781 tagtaaaaca aagcaatttt gaaatgctta acattctcta cggcaatgtc ccgaacgtac
    30841 tctcagaact cctccggaca gcgtttgtac cacgtgaagg gatgaaattt atcgtatctg
    30901 acttctcagc gattgaagca cgtgtgattg cttggctgtc gggtgagaag tggcgtcaag
    30961 aagtattcaa aaatggtggc gatatttact gtgcgtctgc cagtcagatg ttcggtgtgc
    31021 cagttgaaaa gcatggcgtg aatgggcatc tacgtcaaaa agggaaaatt gctgagcttg
    31081 ctctagggta cggaggatcg gtgggtgcat tgaaagcaat ggatgctctt gaaatgggac
    31141 taactgagga tgaattacag ccgcttgtta attcttggcg agactcaaac attaacataa
    31201 cggagctttg gtgggctgtt gataaggcag taaagacttg tgtgaaagaa cgacggagca
    31261 cagcgacgca tggcatcaag tttgaataca agtcgggttt tctgttcatc caactcttgt
    31321 caggacgtaa attagtttat gtaaaaccaa agattggtga gaacaaattc ggcggtgaag
    31381 ctgttaccta tgaaggtgtc ggtgctacca agaaatggga acgtcttgaa agctatggtc
    31441 ccaagtttgt cgaaaacatc gtgcaggcaa tagctagaga cattctctgt ttcgcaatgg
    31501 agaatcttcg taagtatcgc attgtcgggc acgtccacga cgaagtgatt atcgaagtgc
    31561 caatggaatc gaaggttgaa gacatctgtc agttgatgag tcaaacgcct aagtgggctg
    31621 aaggcttgct actgaacgct gatggatacg agtgtgaatt ctatcaaaag gattagtcaa
    31681 tgtggagtca gaaatggctc catatttttt tcaaattttt ttatcaaaaa taaggttcga
    31741 attgggttct ttcttcgctt atagacgaag gcaaaaaaat caaaaataaa ttcgagtcag
    31801 agtttacttt cgaccatttt cttcgcctgt aagttagaag gggcaaaatg cctctcctga
    31861 aaattctatt acaggaggtt cacaagatga acgaattgaa agtttttgaa aatgcggagt
    31921 ttggctctgt acgcacggtg atgattggcg atgtccctca ctttgttggt aaagatgtag
    31981 catcaattct tggctacaca aatccacaaa aagcattacg agatcatgtt gatgaggaag
    32041 ataagacggt gaacgaatcg ttcagcgtta atgggacaat ggctatcctt atcaacgaaa
    32101 gtggattgta ctcactcatc atttctagca agatgccgaa cgcgaagaaa ttcaagcgtt
    32161 gggtgacaaa tgaggtcttg ccgtctatcc gccaacacgg tgtgtacgcg acagaagatt
    32221 tcatcacaaa gtcaatcgaa gatccagcat gggccatttc agtgttgaag cagctacaag
    32281 agaaaaaaga aatggtggcg atgcaacaac aaatgatttt ggagatgaaa ccaaaagtta
    32341 gctactacga tttgattctt caaaacaatt ctgtgatgtc gatttctaaa atctcgaagg
    32401 actacggtat gtcaccacag gcaatgaaca agttgcttca tgaactggga attcagtaca
    32461 agcaaggcaa gatgtggttg ctttatcaga agtatgccaa tcaaggctac acacaatcaa
    32521 aaactcatgc gatagatgca gagcgcagcg tgatgcatac ctactggacg cagaaaggtc
    32581 gtttgttctt gtacgacttg ctgaaatcga agaagaacat cttaccagtg attgagcgag
    32641 gtgataataa tgcctagacg taaagattac agaacatcag tctatgtctg ctcgccgttc
    32701 tcaggaaatg ttgtcgtcaa ccataaaaac actaagcgtt actgccgtat tgctcttgac
    32761 cgtggctaca tgccagttgc ggcacatctg atgtatccac aattcatgga cgacgcaacg
    32821 gagcgtgatg tggcaatgca catgtcactt gttctcatgg gcaaatgtga ggaagtctgg
    32881 gtggtgggtg acaccatctc ccaaggcatg gcagttgaaa ttgaacaagc ggagtattgg
    32941 aagaaacata tcaggtactt cgatgatgag atgaaggagg tctcaaatgc ttaagatgac
    33001 gttattcaca tccaatcagg tgggtaattc caagaatgga ttgttcccaa ccaagcaagt
    33061 ggtgacagat aaagaaacac ttgttgccac ggtcaagctg gatcatgtga tggctgagta
    33121 caaaggcaac tatcgcaaag gtgacaactt catccagtct gatgtgattc cacttgattg
    33181 cgacaatgac cactcagaca atccagatga ttgggtagcc cctttcgaag tggcgatgca
    33241 gtttcctgat gtgacgtttg cggtcagcta ttcacgtaac aacatgaaag acaaaggtgg
    33301 caaatcagca cgtccacgtt ttcacgtata tttcccgatt cctgccacaa ctgataaaga
    33361 tgaatatgtg gaactcaaga agcagattca aagtgagttc ccatactttg acaacaatgc
    33421 gatggatgcg gcacgtttgc ttttcggaac accaaactct gaagttgaaa tctatgaagg
    33481 tagtaagttg atcactgatt ttctcgaaga tgatctgttt gctgattggg ataatgcgca
    33541 gtcagagatt agcgaaggca gccgtaattc aagcatgagt cacatcgcag gaaagctcat
    33601 caaacgctac ggtgcaacgg aagatacgta tcacaagtat ctcgaacaat cccaaaaatg
    33661 taatccgcca ctgccggagg aggagcttaa agctatctgg aatagcgcgg tcaactttgg
    33721 gaagaaagtg aaacaacaag aaggctacat tcctccagag cagtacaact ctgatttatc
    33781 acttgagcca accgactact cggacttagg tcaagcaacg gtcttagctc gcgagtatga
    33841 atgtaagctt cattactcac cgtcaactgg ttaccttgtc tacaacggca gctattggga
    33901 agagtcgaaa ccaaaagcgc aaggtgtgat ccacgcattg acagaacgtc aactggaaga
    33961 atctgaaacc gaaattgaga agcgtaccaa ggaaatggtt agtaatggcg cgttcgatgt
    34021 acttgctagt gttggtccga aaaaggcagt caccatgttc aacacagcgc aacgtcatag
    34081 ttttgatttg taccaacacg cacaagagta taagaagttt gctgtcaaac gccgcgacag
    34141 caagtatctc tcgtcagcat taaccgaggc aaagcccatg cttgaaatcg agcaacggtt
    34201 actagatgtg aacgagttct tgctgaacac accaacagca acctttgatt tgcgaacagg
    34261 caaatcacaa gaccacaact cagaagatta catcacgaag caaacggaat gtgccccgag
    34321 tgacgccaat cagcagattt ggttggacgc tctgaacacc attttcgtag gtgaccaaga
    34381 gttgattgat tatgtccaga tgattgtagg acttgcagct attggtaaag tttacgttga
    34441 agcgcttatc atctcgtatg gtgaagggcg taacgggaag tccacgttct ggaatgtcat
    34501 cagccgtgtg ctcggtaatt actctggcag catctcagct gatattctta caagtcaaat
    34561 ccgacgcaat gtgaaacctg aacttgcgga agcgaagggg aaacgcctac tcatcgcagc
    34621 cgagcttgaa gaggggatgc gactcaacac atctaacatt aagcagctct gctcgacgga
    34681 tgaaattgcg gctgagaaga aatacaaaga cccattcaag tatgtcccaa cgcatacttt
    34741 ggtgctctat accaaccacc tcccaaaagt tggagcgatt gataaaggaa cctggcgtcg
    34801 tttaattgtg attccattcc ttgctacgat tgacggcaat aaggacatca agaattatgc
    34861 ggactacctt tttgaaaatg ccggcggagc cattttgcaa tggatccttg acggtgcgaa
    34921 aaaagtcatc gccgcagact ttcatttaac ggttccacaa gttgtcgcta atgcaatttc
    34981 tgaatataag acggcgaatg attggcttgg ccatttctta gacgagtgct gcgagactgc
    35041 tgttgacttt gaagaaaaat ctggtgagtt gtatgcagag tatcgtgcgt tttgtacgcg
    35101 aactggtgag tacattcgta gttcagccga tttttacacg gctctggagg cgggcggatt
    35161 taagcgccgg cgaactaata aaggaaacaa gattgttggt ttgcaactca aatccgagtt
    35221 cttggaatag ttgattttaa aggaaagtgt aacctgtgta ggtcttatat agaacttttc
    35281 ttaagggata aaaaattacc acctatataa agttatgtat tgcgtattca cgggttacac
    35341 catacttgca gattggagaa tatctaaatg aaatttaaac agtggttaca aattggaatc
    35401 ggtaaacgtg atttacgatt tgatgagttt tacgagtttg ccatgtcgga tgtcacgtat
    35461 ccttggaaga aatcattcga taagcaagtc gcacatctga tgattcactg cgcagaaaag
    35521 gaatacttgg aaatcttgcg tgatgcgtat ttcgagtatt tgacggtgtg gctatgagag
    35581 aaaagcaggt tgagcaagca ctagtgaaag ccgtcaaaag ggtaggtggt atttgcccga
    35641 agtttacatc accggggctt gctggtgtgc ctgaccgatt ggtacttatg ccaaatggga
    35701 aacttggatt tgtcgaagtg aaggcgccag ggaagaagcc acggtcttta cagctttttc
    35761 gtatgaaaca actgacagat ttagggtttc agtgtttcgt gcttgatgag attgatcaga
    35821 tacctgagtt actagaaaga ataggaggtg atgtctgatg aagttcgtac cacataacta
    35881 ccagaaatat gcgattgatt ttatcaaaga tcataaaact gccgcccttc tattggacat
    35941 gggcttgggt aaaacggtga caacgctgac agctattaaa gacttgatgc atgatgactt
    36001 tacaatacaa cgtgtcttaa ttattgcacc gttgcgcgtg acgcaatcaa catggccaac
    36061 tgaaattcaa aagtgggatc atttgaaaga tttatcgtat tccgtggttc ttggaacacc
    36121 aagtcagcga atggcagcat tacaaaataa tgccaacatc tatctgatca atcgtgagaa
    36181 cttggactgg ctgattaaca aatcgggttt tacgtttgat tttgatatgg tggtgattga
    36241 cgaactgtcg tccttcaaaa acttaaaagc taaacggttc actagcttta tgaaagtcag
    36301 acacaaggtt gaccgaattg ttggattgac cggaacacct tcaagtaatg gtttgatgga
    36361 tttgtttgcg gagttcaagg ttctggatat gggcgagaga ttgggctatt atatttcacg
    36421 ttatcgagat aaatactttc tacctgacaa gcaaaatggc atgcagattt attcttggaa
    36481 accaagggaa gatgcagaac aagaaatcta cgacaagatt tcagatatca cgatttcaat
    36541 gaagtcagtc gatttcttgg atatgccaga gcttgtgatg aatgaagtgc cagttgatat
    36601 gggactcaaa gaaaagcaaa agtacgatga aattaaggca gacttggtgc tacaattgaa
    36661 agaagccacc attgatgcag ccaatacagc ggtgctttcg aataaactgc tacaaatggc
    36721 gaatggagca atctatgatg agtttaatgt gagtcatcac atccatgacc agaagttaga
    36781 tgctttggaa gatttgattg aaggtacaaa tggcaaaccg attctgattg cctattggtt
    36841 ccagcatgat ctggagcgta tcaaggaacg attcaacgtt cgacagatca aaacgtctca
    36901 ggacattatt gattggaacg aaggtagcat ccccatcgct gtcattcatc cggctagtgc
    36961 aggacatgga ttgaatttac aagctggtgg ttcaacactt gtatggtttg gtcttacgtg
    37021 gagtttggaa ctttaccaac aaaccaatgc aaggctgtgg cgacaagggc aaagtgatac
    37081 tgtggtcatt catcacatta ttgcgaaaga tacgattgat gaagatgtga tgttggcttt
    37141 gaaacgaaaa gataaaacac aatcatgttt gattgatgca gtgaaagcta gattggaggg
    37201 aagcaagtga caccaaaaga atacttgaaa caagcgtacc gtttgaataa acgaattgac
    37261 gaccggatta aagagaaggc ggagctctat caactggcgg tgacagtttc ttccacaaca
    37321 ttcgaagaaa agatttcaag tggcacacgg aatactgagg caccatttgt tcgtaacctt
    37381 gagaagtgtt accaattgga agctgaaatt gatgcagaga ttgataagtt tgtagacctc
    37441 aaacgacaaa tgcgtggcgt gattgatgaa ctcacaaacg tggacgaaaa gattgtgtta
    37501 atcaatcgat acctgttgga agagagctgg gacaagattg ctgaagatct tcacgctgac
    37561 gtgcggacag tctttcgttg gcatagcaaa gctcttgaga acttgaagct accagatgaa
    37621 cccatagtca tttgaaatgt gtcagtgaat gtcagtagcc tttagtgata tcattaagat
    37681 gtcaaaaaag acaaacgata ctcaagcaat accagcaata gaagtatcga acaaatactc
    37741 gaagcctttg tgggaaaaat ccttcaaggg cttttctttt gcccgaaagg atgtgacccc
    37801 acttgccaat gaaaccagct aacccctgta agttcccagg ttgtccgaac cgtacgcatg
    37861 atacttattg tgatgaacat aaaacacttg cgaggaaacg ttacgagaaa tacgagcgtg
    37921 atccggcaat caacaaacgt tacggcagag cgtggaagaa aatacgggca aggtacgtgg
    37981 cagcacatcc cctatgtgag atgtgtctga aggaaggacg ctttacttcg gttgagattg
    38041 tgcatcacat caaggaatta agtgatggtg gtacgcatga tgaaaataat ttgatgagtg
    38101 tctgcaaatc atgtcactcg aaaattcaca tgactcgacg aaacacaaaa gataaaacaa
    38161 tcatgtgaaa aataacaagg gggggagggg cggtaattat ccctaaaccc ttctaaactg
    38221 tacagcgcgc taggccctta cgcgaaaaaa gttcagttca aacgggggat agccccaaac
    38281 aagaaaaaga ggtgataaat tttggcaaaa gatggaacac aacgcggtgg cagacgacct
    38341 cgttcagggg aacgctcaca gccactcgtt gataaagttg ctgctggaaa gaaagcatcg
    38401 cggatggaat ttgacttacc ggatgttgaa ttattaattg gtgaagatat tggtgagggt
    38461 gtagagcttg agggcaatga ttatcctgaa ccaagtgctt acctttctgc aaaacagaag
    38521 gatggcactg tgctaggcgc tgatgaaatt tataactcaa cgttccaatg gttgaaagac
    38581 cgcggatgcg aaacgttgat agccccacga atggttgagt cctacgctca gaactttgcg
    38641 cgttttgttc aatgtgaaaa tgctctgagt caatatggtt tgctcggaaa acacccgact
    38701 accaaggcag tcatggcctc cccatttgtg gcgatgtcgc agaacttcca aaagcaagca
    38761 aatgtgacgt ggtatgagat tcaacagatt gtaaaagaga atgcatcagt ggaatacaca
    38821 ggagatccga atacggatgc gatggaacga ttgttacgtt ctaagaatgg ttgaaaggaa
    38881 cagaatttat gatagagaag gttaatccgc aacatccaga taaagttgca gatcgtattg
    38941 ccggagccat tgtggacttg gcatacgcag aagaaaataa tccaaagata gcagttgagg
    39001 ttttaattgg tcatggcatc tgtcatgtga ttattgaaac ttcaactgct ttaaatttga
    39061 atgaaatcag ccaagcgatt aatcgtatcg caggcaaaat taaaaaagat gtggtgattg
    39121 tgccccaaga taaagagttg gctgacaatc aagcgactga aattcgttgt ggcgataatg
    39181 gaatctttcg tgggatgcca ttgactgatg aacagttaca gctctcaaaa attgcgcgtg
    39241 acttatatca ggaccatcca tttgatggaa agtacattct tgatggtgaa cgcttgataa
    39301 tttgtcagag ccacgcaaag acaaatgagt taaaacaaaa atatccaaac gctacagtca
    39361 accctctagg tgattggact ggcggcccgg atgtggatac aggtgcgact aatcgcaagc
    39421 ttggttccga tatggcagac ggagtaacag gtggcggtat ccatgggaaa gacttatcaa
    39481 aagctgatgt gtcagtgaac atctacgcat tcttgaaagc tcaagaaact ggtatgcccg
    39541 ttgagttaag ctgtgcgatt ggtgacactg agattgatgg cgtgccttat tctgaaatcg
    39601 tggaagttgc gcgtgattat gtgaatgagc ttggcgggtt tgagaaacta gcagaatggg
    39661 ggttgttcta atgaacacaa cagaacgttt tgagaaagtt gatattgata agctggtccc
    39721 ttatgccaga aatgcccgta cccacagtaa ggatcaagtg aaacagattc aagcttctat
    39781 gcgtgagttt gggttcataa atccggtgat tgttgataag gattataact tgattgcagg
    39841 tcacggacgt attcttgcgg ctaaagaaga aggtatcaag caggtaccgt gcgtgtttgt
    39901 ggaacaccta accgaggcac aaaagcgtgc gtacatactt gctgataacc gactagcaat
    39961 gaacgccggt tgggatgatg aaatgttggc ggttgagtta tcagacctgc aaggtgaaga
    40021 gtttgatttg gatttgcttg gttttgaaac tgatgaactt gataaattac tcaacggtaa
    40081 tgatgatgcc caagatgatg actttgatgt agatgaggaa cttgaaaagc cagccttcag
    40141 taaaacaggg gacttgtggt tgcttggtcg tcaccgttta gtttgtggtg actcaacaaa
    40201 acctgaaact tatgagaaac tcatggacgg caaactagca aatcttgtgg tgacagatcc
    40261 accttatggg gtctcatatc aaggtaaagc gggaacgatt aaaaatgata atctgaagga
    40321 cgaggcattt tacaacttct tgtatgatgc cttttcttgt atggaaagtg tgatggccaa
    40381 cgacgccagt atttatgttt tccatgcgga tacggaaggc ttgaacttca gaaaagcatt
    40441 cgtggatgca gggttctatt tatcaggtac gtgcatctgg aagaaacaga gtctagtgct
    40501 tggtcgttcg ccttaccaat ggcagcatga gccggtgctc tatggctgga agaagaaagg
    40561 caaacatcaa tggtacaccg gacgtaagga atcgaccatc tgggaatttg ataaaccaaa
    40621 gaagaatggt gaccacccaa cgatgaaacc aattccgctg ttggcatatc cgattaccaa
    40681 ttcgtcactc agcaactgca tcgtgttgga tagttttggt ggcagcggat caactttgat
    40741 tgcttgcgaa cagaccgacc ggatttgcca tacgatcgaa ctggatgaaa agttctgtga
    40801 tgtgattgtt aagcgttata tcgagcaggc tggaagcgat gcagacgttt cagtgattcg
    40861 agatggaaag actatcgcat ttaatgacct agaaaatcct gaataaataa cgtatcactt
    40921 gctataaaat ccctttagag tgatatatag actaccaaaa gaatacggaa gacaaagtta
    40981 tgtttccaac tcatgaacaa atcgagggta tgaaaaaacg ttaccctgaa ggtagtcgtg
    41041 tagaactcat caagatgaac gacccacatg ccccagcgaa gggcacacaa ggcacagtaa
    41101 ccggtgttga cgacattggt tcaatcctta tccaatggga caacggttca acactgaacg
    41161 cggtatacgg cgaagatgtg gtgcacatca tcaaaccaga cttcgaattg gtctttcaaa
    41221 acggtagaag agaaggattt gaaaccttca atgatgcttg gcaatacgtg acggacatga
    41281 tattgaacca cgatttggtc tgggttgacc ttgagtcaaa aacagaagat accatctaca
    41341 tcagaaaggg gctttaagat gaaagtgagt aaacgtaaga tttacaacat cgcaaagaat
    41401 tacatcgttc agttgcctga acgtggcgac ctgaaggccc acaacagcga ccgtgaagac
    41461 ttcttggaca ttgcggtttg gtgtttggaa gatgccttaa ttgcggccta tgaggcaggc
    41521 agaaaggacg gtcaaaatga acgcaaagat taagcaacaa atcattgaga ttgcagaaag
    41581 tggtctgaca aatatgtttg atgcgattgc agtgcagcga attgcacatg accggcactt
    41641 ttacgagttg gtgattttca ttgaagaaca caaagatgag tacagccgct ttatcttgga
    41701 gggtgataaa tgatggatta tcaaatttac atgccagata atgaagatgg cattgaagaa
    41761 ggatactacg attggcaaga actggtgcaa ttgctgcggg ataacaagaa caaccctgaa
    41821 gccattcagt tcattgccga tatgatggaa taaagagatt aaggcagctg acaacagctg
    41881 ctttttgcgt agaagggagt gagttccaat cgataattac aaaccaactg aatttatgga
    41941 caaagactcc cattatgata agtcacgtgc agattatgcc gtggccttta ttgagttgtt
    42001 gaaacacacg aaaggtcagt ggttcgggaa accgttcaaa ctgatgcctt ggcaagaaaa
    42061 gattatccgt gacttattcg gaacgattaa ggcaaatggc tacagacaat tcaatacggc
    42121 ttatgttgaa atacctaaga agaatggtaa gagtgagtta gcggctgccg tggctttgct
    42181 tttgacttgc ggtgatgggg aagaacgcgc tgaggtatat ggctgtgctg ctgatcgagc
    42241 gcaggcttca atcgtatttg acgtagctgt tgatatgatt cgccagaacc cagcgttaag
    42301 caaacgagtg aaaatcctga cttcacaaaa acgaattatt tatcagccaa cgaatagttt
    42361 ctatcaagta ttatcagctg aagcatactc taaacacgga ttaaacagtt ctgcggttgt
    42421 atttgatgag cttcacgcac aacctaacag aaagctgttt gatgtcatga cgaaaggggc
    42481 aggggatgct agaacgcaac cgctattctt tttgattaca acggcaggga atgataccaa
    42541 ttcaatttgt tatgagcagc atcaaaaagc tctcgacatt attgaaggtc gaaagcatga
    42601 taaaacattc tatccgttaa tttacggtat caagcaagat gatgattgga ctgatccaga
    42661 agtttggaag aaagccaatc cgtcacttgg gataacgatt gcagaagaaa aggtggtggc
    42721 tgcttgtgat tcggctaaac aaaatccagc tgaagaaaac tcattcagac agcttcgcct
    42781 gaaccaatgg gtgaaacaag cagtgcgttg gatgccaatg gataaatggg acgcttgttc
    42841 attcaatgca gatgaagaaa gtttgagagg tcgagtttgt tacggaggtc tcgacctttc
    42901 cagcacgacc gaccttacat ccttcgtttt ggtgtttcca cctgaagatg aggatgacaa
    42961 gtttgtgata ctgccatatt actgggtgcc agaagatacc ttggacttac gagttaaacg
    43021 cgaccatgtg aattatgatt tgtgggccaa gcaggggtac atacaaacca ctgagggaaa
    43081 cgtagtccac tatggtttca ttgaaaaatt tattgaggaa ctgggtgaga aatacaatat
    43141 ccgggagatt gcctttgacc gttggggagc agttcaaatg gttcaaaact tagaaggtat
    43201 gggatttact gtagttcctt tcggtcaagg tttcaaggat atgagcccgg ctactaaaga
    43261 gttgatgaag ttgactcttg aacagaaatt agcccatggc ggtcatccgg ttcttcgttg
    43321 gaatatggac aacattttcg tccgaactga cccggcaggt aatatcaaag ctgataaaga
    43381 aaaatccact gagaagattg atggtgccat cgcgacaatt atggctctgg atcgtgcaat
    43441 tcgttgtgga aatgataatg gtgccagtgt ttatgatgat aggggattgt tgtttctgtg
    43501 aaatatgagc ttttattaca atccattgat ataattgtct ttggaggtgt atgtgtatga
    43561 atgaatattt gattcaagta gcaccagata aaatatcttc agacccggac aacacattaa
    43621 tttctagact gttttataga aaatggtggg gaaaaattct aactgaagat ttgacaaagt
    43681 atgctgaagg aattgggaat tataggggag aaacgattat ttctttcaac acaattgctg
    43741 gtgcaacgat acggttattg aaatgctata atcaaaagaa cttttcaatg ccagatagtc
    43801 attatgggcg tttgaaagtc attttatatt ccgatgatat caccgaagag atgaaagtga
    43861 aatttgtaaa attctatgat ttaagtcata ctcttgcaaa ttttatgcca ctatatgtat
    43921 caaaaccttc tctcaatggt gttaaaggta aagggccgta tcatgacttt cctgatttat
    43981 ttta
//
LOCUS       DAZAJZ010000011        42268 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000011 DAZAJZ010000000
VERSION     DAZAJZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.011"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..629)
                     /locus_tag="ACG86D_000630"
     CDS             complement(<1..629)
                     /locus_tag="ACG86D_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139805.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKD"
     gene            831..1130
                     /locus_tag="ACG86D_000631"
     CDS             831..1130
                     /locus_tag="ACG86D_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139806.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1395..2270
                     /locus_tag="ACG86D_000632"
     CDS             1395..2270
                     /locus_tag="ACG86D_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139807.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            2267..2485
                     /locus_tag="ACG86D_000633"
     CDS             2267..2485
                     /locus_tag="ACG86D_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139808.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2472..5099
                     /locus_tag="ACG86D_000634"
     CDS             2472..5099
                     /locus_tag="ACG86D_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFN3139809.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            5114..5332
                     /locus_tag="ACG86D_000635"
     CDS             5114..5332
                     /locus_tag="ACG86D_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139810.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6851..7165
                     /locus_tag="ACG86D_000636"
     CDS             6851..7165
                     /locus_tag="ACG86D_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139811.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(7218..7991)
                     /locus_tag="ACG86D_000637"
     CDS             complement(7218..7991)
                     /locus_tag="ACG86D_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139812.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            8096..8341
                     /locus_tag="ACG86D_000638"
     CDS             8096..8341
                     /locus_tag="ACG86D_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3139813.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            8497..9348
                     /locus_tag="ACG86D_000639"
     CDS             8497..9348
                     /locus_tag="ACG86D_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFN3139814.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            9381..10058
                     /locus_tag="ACG86D_000640"
     CDS             9381..10058
                     /locus_tag="ACG86D_000640"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFN3139815.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            10546..11511
                     /locus_tag="ACG86D_000641"
     CDS             10546..11511
                     /locus_tag="ACG86D_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFN3139816.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            11533..11745
                     /locus_tag="ACG86D_000642"
     CDS             11533..11745
                     /locus_tag="ACG86D_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN3139817.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11820..12296
                     /locus_tag="ACG86D_000643"
     CDS             11820..12296
                     /locus_tag="ACG86D_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFN3139818.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            12293..14101
                     /locus_tag="ACG86D_000644"
     CDS             12293..14101
                     /locus_tag="ACG86D_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFN3139819.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            14178..14393
                     /locus_tag="ACG86D_000645"
     CDS             14178..14393
                     /locus_tag="ACG86D_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFN3139820.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            14485..15348
                     /locus_tag="ACG86D_000646"
     CDS             14485..15348
                     /locus_tag="ACG86D_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFN3139821.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            15385..15921
                     /locus_tag="ACG86D_000647"
     CDS             15385..15921
                     /locus_tag="ACG86D_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFN3139822.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            15935..16357
                     /locus_tag="ACG86D_000648"
     CDS             15935..16357
                     /locus_tag="ACG86D_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFN3139823.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            16287..18686
                     /locus_tag="ACG86D_000649"
     CDS             16287..18686
                     /locus_tag="ACG86D_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFN3139824.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            18718..20709
                     /locus_tag="ACG86D_000650"
     CDS             18718..20709
                     /locus_tag="ACG86D_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFN3139825.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            20732..20983
                     /locus_tag="ACG86D_000651"
     CDS             20732..20983
                     /locus_tag="ACG86D_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFN3139826.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20973..22202
                     /locus_tag="ACG86D_000652"
     CDS             20973..22202
                     /locus_tag="ACG86D_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFN3139827.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            22199..24280
                     /locus_tag="ACG86D_000653"
     CDS             22199..24280
                     /locus_tag="ACG86D_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN3139828.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            24429..28349
                     /locus_tag="ACG86D_000654"
     CDS             24429..28349
                     /locus_tag="ACG86D_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFN3139829.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            28350..29294
                     /locus_tag="ACG86D_000655"
     CDS             28350..29294
                     /locus_tag="ACG86D_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139830.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            29298..29501
                     /locus_tag="ACG86D_000656"
     CDS             29298..29501
                     /locus_tag="ACG86D_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFN3139831.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(29801..30037)
                     /locus_tag="ACG86D_000657"
     CDS             complement(29801..30037)
                     /locus_tag="ACG86D_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139832.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(30220..30591)
                     /locus_tag="ACG86D_000658"
     CDS             complement(30220..30591)
                     /locus_tag="ACG86D_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN3139833.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(30588..31916)
                     /locus_tag="ACG86D_000659"
     CDS             complement(30588..31916)
                     /locus_tag="ACG86D_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN3139834.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(31877..32206)
                     /gene="mobC"
                     /locus_tag="ACG86D_000660"
     CDS             complement(31877..32206)
                     /gene="mobC"
                     /locus_tag="ACG86D_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFN3139835.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(32464..32834)
                     /locus_tag="ACG86D_000661"
                     /pseudo
     CDS             complement(32464..32834)
                     /locus_tag="ACG86D_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            32979..33164
                     /locus_tag="ACG86D_000662"
     CDS             32979..33164
                     /locus_tag="ACG86D_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN3139836.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            33322..33489
                     /locus_tag="ACG86D_000663"
     CDS             33322..33489
                     /locus_tag="ACG86D_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139837.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            34107..34766
                     /gene="vanR-B"
                     /locus_tag="ACG86D_000664"
     CDS             34107..34766
                     /gene="vanR-B"
                     /locus_tag="ACG86D_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN3139838.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            34769..36130
                     /gene="vanS-B"
                     /locus_tag="ACG86D_000665"
     CDS             34769..36130
                     /gene="vanS-B"
                     /locus_tag="ACG86D_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN3139839.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(36221..37522)
                     /locus_tag="ACG86D_000666"
     CDS             complement(36221..37522)
                     /locus_tag="ACG86D_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3139840.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            37801..38607
                     /locus_tag="ACG86D_000667"
     CDS             37801..38607
                     /locus_tag="ACG86D_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN3139841.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            38625..39332
                     /locus_tag="ACG86D_000668"
     CDS             38625..39332
                     /locus_tag="ACG86D_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN3139842.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            39329..40300
                     /locus_tag="ACG86D_000669"
     CDS             39329..40300
                     /locus_tag="ACG86D_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN3139843.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            40293..41321
                     /gene="vanB"
                     /locus_tag="ACG86D_000670"
     CDS             40293..41321
                     /gene="vanB"
                     /locus_tag="ACG86D_000670"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN3139844.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            41327..41935
                     /locus_tag="ACG86D_000671"
     CDS             41327..41935
                     /locus_tag="ACG86D_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN3139845.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(42001..42174)
                     /locus_tag="ACG86D_000672"
     CDS             complement(42001..42174)
                     /locus_tag="ACG86D_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139846.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        11980 a         9496 c        10521 g        10271 t
ORIGIN      
        1 atatctttat aggctaagtc catttcgtct aatagctgtt ttaaagaatc aatcattctt
       61 tctttttcac cgtatttgaa atcaccattt cttatcttgt cttgaagttt actgttggct
      121 cttttgaaaa atagctcttt agagatagag tgtctttggt tagcgtactc ttcaatcaga
      181 ggattgatat tattagcaag tatctcattt agataaattt caaaatcttt tgagtaatat
      241 tttgtaaata tattttttat tataggattc aataactcat agtctttatc tagcgaaaac
      301 gtatcactat atctttcttg aatgtcttta aagactttgt gaggtatttc tgggaagtcc
      361 ttaatatata gctcataccc tatatcgata aggtatgaag ttatatattc ttctaaagtt
      421 ccccatttaa tccagtcaat tgttgaatta gtgatagttg ataataattt cagcttttgt
      481 ggatttggta aattggtccc acgttcccag ttggatatgg tcgcacgtgg tgaattatct
      541 attttccttc caagttcttc catagtaaaa ccacatgaaa ggcgtaattg tttgattcgt
      601 tttcctgctt ctgaaggatt tggcttcatc ttttttctcc tttttatttt taaagatagt
      661 atatcatatt tataaaataa aagtatttaa aagtatttac tttttatctg gagtatgata
      721 ctttattctt ataaatacaa ataaatacat ttttttacgc aatttgtatt tatttgtact
      781 tataaaggta gaggagttat agaataatta attaaaaagg agaggttttt gtggaagcaa
      841 taaaattaat gatttcagaa gaacaggagg aaatgtttct aaagcagttt tatcaaattg
      901 ctacaaaagc tattgaaaag gctcaagaaa atgcggggat gacaagggaa tatctcaatc
      961 aaaaagaaat ggcggaatac ttaaatgtga gtgaaaactt tttaaaaaat gctgtgagaa
     1021 atgagggatt gcccgtagtt gaattagggg caagaaaatt ttattcgaaa aaggcagtta
     1081 atgagtttat gtttgctcga atgaaaagag gggaaaatat tggaagatga tgaagtagaa
     1141 attataaaaa aatgctttaa aaaaagaaaa aaagtgttcc caaaagtttg cagactgggg
     1201 gaacactcaa taataaagtg ctgagaaaac tttatttata gctattatat caaaaaagaa
     1261 ggtgaaaagg tgttttcttc tggaaatgtt gtagttgaca aacttagaga aataaatttt
     1321 gacggaaatg taatccacac acatggtacg atcaattaag aaaaaaaacg aaaaaaggtg
     1381 ttgagaagcc ctatttgaat gcaatagtta tcttagcaga aattacctat tggtatagac
     1441 ctaaaaaaat ttttaacgat gatggtcagc tacttggtta tcaaaaaaag tttcttgaag
     1501 atattttgca aaaatcatat aagcaattaa gcaaaaaaac aggattatca gaacgagcta
     1561 taaaagatgc aattgtgtac ttagagaaaa aggggattat caaaagggtt tttagaaata
     1621 tttattatga gaaaagagca gttacattat caaatgttat gtacattgtt ttaaatgtac
     1681 ataaattgat agaaattact tttcctactt tggattgcga aaaaaaggag tatatgccga
     1741 aaattcgtca gaggggtatc gaaaaaaaga gatatgtctc tttaaaaaaa ggtagaggta
     1801 tgtacaaatt tgtatatacg tatacaaaga ctacttcaca gaacatatca aagactactc
     1861 tcgttatgga agaagaggat attaatataa agaggattta cagtaagatt agtgttcttt
     1921 atgacgcaaa taacgaagat ggtcgaaaat tatttcaagt gtatcactat ttgagaaacg
     1981 ctaatgtaga tacagaagat atcagaaaaa tcattgaata tttatctgaa aatactcagt
     2041 atcttattgc tgagtgtatt ataaaacaac atgaagcatg cttttttcaa agaaaaactg
     2101 aagaagggtt atacgattat gtaacatatt ttttgaatgg acttcagaaa agatataaaa
     2161 gtcaattttt agaaaatgta gagcctatag atcagttcaa aaaagctttc gaaatcaaag
     2221 agttacctga aattactatg cataattggt tgaaaggaga agaatcatga ttaaaaatcg
     2281 cttaatagat caaatgtggt gtcttgatca aaatacagat actctcgaat taatcgagag
     2341 tatctgcttc aacacagtag ttctcgattt aagagctgaa aatgacaatt gtgtaactca
     2401 tatcataatt aatcaaaaaa tggctcagca gctctctgag agcctaagaa aatgggctga
     2461 aggagaaaac tatgaaagca attaaagaaa aaatcgttag tagaaaaata aactggttag
     2521 cactcagtgt acttgtttta atgattagcg ttgtttttac aagtaatatt gttgaagcta
     2581 gcattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt
     2641 atagacctta cctaaaaaaa tacaatggag atttccttag tcagttcggg atttggcttg
     2701 gatgggctgt ggtaaaaggc atgtttactg tgactgattc gattcaaaat atgattccag
     2761 atgtgttgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag
     2821 tcataaatac aattgtcgtt ggattaatga tcctttcatt gatgtttgtt ggctacaaga
     2881 tgattactgg aaaaggaaca atagatttaa gatctgttgg aatgaatata gtgatgtctg
     2941 tagcgttaat tcttttaatg ccaacaatga tttctagcgg tattcaattt gccaaaatat
     3001 tttacaatga tgctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa
     3061 aacaaggagt aacagatctg gcatatatta ataaaacaga tcaatactca agcattgata
     3121 aaacggagga tcgaaataag ttaacacgaa aaaattttca acagacagat ttaacacaag
     3181 tgttgacgga taaagtgatt gataaattgg aaaaagaaaa tccagctgcg gataatcttc
     3241 gctatgaatt agttgaaaat tctaataatg aatttgttgc cacaaagttt tctgataact
     3301 tcttatcaac attttctgat agcttgaaat caggttatta tcgatatcaa gcaaatcttt
     3361 gggggatttc gattgggttg acagcgttgg caatcgccta tgtgttcagc gcgtttgtga
     3421 tcattacagc aatattagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcga
     3481 cagatattga aacaggacaa cgttcaaaag ttgttttatc cgatatttta caatgttact
     3541 taacagttgg atttcaaggg tttggactat ctatgtttgc tatgtttatc aattttttaa
     3601 atgctggaca aggtatctcc acaaatattt ttataaaaac aattgcttat atctgtgctg
     3661 tgtttgtgct gataaaaggc agtggaacag tcatgagata ctttggtgtt gatattggac
     3721 tcaaagaagg ctatgggcaa cttgcttctg cttttggtat gggcgcaatg ctgtttagaa
     3781 aaggatcaac tggatttaat cgtgctaaag gaagcggaaa tggaaatgga tcagacagtg
     3841 gagaaggaga agatcgaaaa ccagaaaaga atttcggcga aactctatca aaaaaagctg
     3901 gcaaaactgg aagagctttg ggatatgccc atgaacgtgg attatcaggt ttagcttcag
     3961 atggagctac aatggctagt gaaagagcaa taaaaccatt taagagtatg cgtgatatgg
     4021 ctaatgatac caagaataag ttcaaagaag gtttagatga tggtacagtg tccgctatta
     4081 ataaaaatag caaacctatg ttagctaaaa ataaagaaga tgaaactgga aaatatgctg
     4141 attccatgcc aacacgtctt tcggatagaa aagatggtgc taaggttgaa aatgccgata
     4201 gaattatgtc tagttctgaa cgtatgcgtg aagcaatgaa aaataatgct gaatcgaata
     4261 ataatcctgt aagtgcaatt cagcaaaaag ttcaacaaga tattgaagaa cgtaaaaatg
     4321 ctatgcacgg tcaagctaag tctgctgaag aattaattaa tcagaagcga caagaagcta
     4381 aatacacgcc tgaagcaatg aatcgtgagg aaatgttaag gaaacgtgta gaagggcgta
     4441 caggagctaa tacggacgaa aaagaagcgt tgaccaaaga aagaatccaa gaagctaaaa
     4501 actcaaatac cggattagaa aaattggtca aagaaaatct acaaaattct acaagtagtc
     4561 aaggtggccg atcagtagat gtccgagaaa atattcaagg ttcattgaat ggtcatgtcg
     4621 gacggaatgt tgatgtaaga gagaatttac aaagctcttc aaatggtcaa ggtactaaga
     4681 caattgatgt tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt aaatcaatgg
     4741 atgtaagaga gaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa
     4801 gagagaatgt acaaagttct acaagtagtc aaggattcaa aacagtagat gttcgggaga
     4861 acgttcaaaa agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat atcgttgaag
     4921 acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac agtgattatt gatagtgaaa
     4981 tccgtgaaaa tgataaggga tcaaaaccaa gacgaagatt tacctatgaa gacaacgagt
     5041 tattcagaga tacacttaat gatcctaatc cgcttttcga taatctatta aaaaaataaa
     5101 agggggaatt gtcatgaaat taattctaaa gattagtacg catgatttct tagaacttga
     5161 cgaattatgt gaatcagtaa agaacgcaac aaatgaaaaa atcacggtca ctaattatat
     5221 tgaactgatg attcaagaag aattaaagat gaaacatgag ataaaaaaca acttgaaata
     5281 ttgttatcta gaaaaaagtg aagagataat ccatgaatta aatggctttt aaaaattgta
     5341 tttgacatga atttacgaca aggaattagt ggggattaag cgattattct cttccacttt
     5401 agagaaataa tgggttaaaa agccagaata cgtagagcaa tattgacaac ctataaagga
     5461 gctattgttg aaaattgaag taacaaaaag ataaagggag gtttacatct atcagattaa
     5521 aagtgatagt acacccatga aacctgcaaa agagttgaac ctaatgtata tcggtttaac
     5581 tctttttatt tgcaaattaa aaaaatatca ccgaagtgac gaatgaaact atgaccaaat
     5641 taagcaagaa aatatatctt gcaaataaag tagtataaaa agatattgat aaagccaatc
     5701 aaaactttga atttcaaacg ttagataatc aaacagatac aaaaaatgaa atcagttcta
     5761 cgcgtacgat tattaaaaat aataaatttt cactagaaga aaatggaaat aaaatcaaat
     5821 aattaggaag aaaaaacaaa gcaattagaa aagcctgcaa gcaaatttga gtaatcgaga
     5881 tccaattttt ttaaggaagt ttaacaaaaa tatgtaattc tattatcact attaaagaga
     5941 aagttggagc ggaatataaa gaaaagagaa aagtaattaa acgcaaaaaa atgtagaact
     6001 taaaaaatat aaagaggaac aaccgttaaa attaggaatg atgttgtgta aacattataa
     6061 ttgctatgat ataaatgaag tgttaaagaa agcaaattta aaccaatttt gtgaacagaa
     6121 aagccttagt cactatgacc aaaactacgg acgaaactat gaccaaaagt aggtactaaa
     6181 ttatgaccga aaataggtcc aaaatggatt tttttatgat atcaaaaatt atgaaatgat
     6241 tttgaaagaa aaccagttgg cacagccaac ttagccacct tcctgtagga aggtgctatc
     6301 agttttccct tatgctcttt tctttctttt ttgtttcata tgtcaaatgc cattttgaaa
     6361 tgtaccatct taagaaattt atccttaaaa acaattgcgt tattggataa aaagatgcta
     6421 cattatctta aaaaaggaat caaatatgat gaatttagtg gctagcatat cgtgtgatta
     6481 agatacttta tagtgtaagt tgaaggagaa aaaatattta aaatatactt cccatttcag
     6541 tgggaagtaa aattaatctt gttgtttcta ctagtaatgt atctttttgg aaactatgta
     6601 acgatgtaaa caagattttt ttcagaagaa aaaacgataa aagttgtgat aaacgattta
     6661 aaaaatagga gaaaatatat caagcgcatg gtatgaaacg cacatttaaa aagattcgac
     6721 taactaaaaa aataagttta caaagcattg ttgtgtatta caagtgatct atacactgaa
     6781 aaatgataga gaaaatctga tacgtccgca acaatggaca attgaaaatg aaagtgagga
     6841 aattggaaga atggctgatg aaaataaagt tgttccgctt gaaacactag tgataaataa
     6901 ggaagagttt gagcaacttt ttcttgattt ttttgttcaa tatgacagga tttatgattt
     6961 tagttcgtca ctagatttat ttgaattaga ggcagaaata atagaaaaca gtcgagagga
     7021 gaatggtaaa atcatcattt cagtagaaaa attcaatgaa ttattctcag aaacatttga
     7081 aatagataca gattattatc aaccacaaga agttttccaa tcatttagaa atcagattat
     7141 tcgctcaggt aagaccgtaa actgaaaaac aataaaagaa acgttgaaag aataattatt
     7201 ccttcaacgt ttctttttta gaatgtttta atgaccaaag cagtttcatt gtcaggctta
     7261 ggtaattcat gaaatcccgt atttatagtg ctcaattgat caactaacca tgttttactt
     7321 tttgaattgt agataaatct aaaattaata tcgttattat cgtgttgaag gggcgtctcc
     7381 aaataatctg ctgtatattc atagtcaacg taacctgcta atacaaaaga gagtatccct
     7441 ttatttaatg ttagatttaa tggataatca tacgacttta tttttttcaa cttcatattg
     7501 tacatatctt cagccataaa agttgagttc gtgatttcat cttgggtctc attaaaaaag
     7561 ttatccgaat aatcgcttat ttttgaaaag tctttattgg caccagcctc aaaaaattgt
     7621 tcaacttttt cagtataaag ttgtgctaac gctccttttt caatattaac tttaatattt
     7681 tcatgtctat caatttgtgt gctgttcagt aagggaagat cagcttttag ttttatatcg
     7741 tcttgatttc tttcaatttc aattggcaaa atcttttcag tagattcagt ttctgataaa
     7801 ggaacatcta gttttttatc atgataataa gcttcaaatc catctggaac cggaaaaaaa
     7861 tgtattgggt aattcttttc tgaggttgtt gtactagttt ccgtacttgt agaacttgtg
     7921 tcagaagtgg tacacccgct tatagttccg atgagtaaag cgcaagttag aatgattgca
     7981 aatttcttca aaaaaattcc tccttaaaat tgaatatgga ctaatactag tcccattgta
     8041 gcataccgaa tctttaagat taataagaaa aaggactaat tttagtccaa aaggtgtgct
     8101 attcttgaaa gaggaaaaaa ttattgataa agaaaatgta atcggtagca atattagacg
     8161 agtccgatta gaaaaaggaa ttggtcaaac cgagctgatt agagacctac agcttcgtaa
     8221 aattgctata actagggaaa cgttggttaa gattgaaggt ggccgccaac acattaagtt
     8281 ggatcaattg aaagccatca aagatattct acaggtgtct tatgaagatt tgcttcaatg
     8341 actacttgta gtcatttcgt ttgaaattat tatgttacac ccttatgtgt cacgaagaaa
     8401 tactggtata ataatgaatg ggatatgtaa cgatagggta tactgttatg ttgcctaata
     8461 aattgtggat caaaatacaa gaatggaggg atatccttgt ataaggcata tgaattggat
     8521 tttacaaatg ctgacttaaa tgcattctcc tcaagctcgc aatcatatac acatgtagta
     8581 aatcaaatag atcacaacca aaaatctatt aacaagagaa tagaaaatct atttgaaagt
     8641 gaaaatgatt tagttgatta ttattctaag gacagcaaaa ttctagatgc agacgaaatt
     8701 attaaagatt ggtttccaac tataaaagca gatattttta tatctcactc tcatgctgat
     8761 gaaaaattag ctatacgttt tgcatcatgg ctatttgaaa attttggtct tactgcattt
     8821 attgattctt cggtttgggg atacagttct gatttattaa aaaaaataga ccaaaaatat
     8881 tgctataagg aacaaacaaa aacatatgat tatgataaaa gaaatgtaac aactagtcat
     8941 gttcatatga tgctttctac agctttaaat aatatgattg atagtacaga atgtctgttt
     9001 tttcttaata cacctaattc aattagtttg agcaatgaaa tcactaatga acagaaattt
     9061 acgtattcgc catggctata tagcgaactg acgacagctt caatagtgga aaaaaagaat
     9121 ccacgattgg aaagtaatcc tcaaatgagc accgaggatg ttagaagcat tataaagcac
     9181 tattcttcgc aagaaaatct gcaaataaaa tattctttaa atttaagtga attaacttat
     9241 ttacgaaaag ctgatttgaa aaaatgggct ttagatgctg tggaatgcac taaaggaagt
     9301 aatacattag atattttata taaaaacacc attttcacta atctctaatc aatttactga
     9361 atcgaaagga accgggtaga atggataaga aagttttaat aaagaggttc tctgaagcta
     9421 ttgaaaaagg aaatgcagca atatttgcag gtgcaggatt atcgatgtca caaggatatg
     9481 taagttggcc ggaactactt aatgatcctg caacagaaat cgggttggat tctaaaaaag
     9541 aaacagactt agttactctg gctcaatatt ataaaaatga aaatggtgga tcaagaggaa
     9601 ttttgaatca gatacttatg gataattttg gtgaagaatt agaaatatct gaaaatcatc
     9661 gaatactagc aagtttacct attgaaactt attggacaac taattatgat catctaatag
     9721 aaaaatcaat ccgagaagct tataaaaacc ctcaggttaa gaaaaattat actcagttag
     9781 caacaactaa tccaaatgtg gatacaattg tttataaaat gcatggtgat atagatgatg
     9841 tcagttcgac agtaataaca agagatgatt atgaaaaata tgatgattcg tacgcgttat
     9901 ttaaagaaac tttaaaagga gatttgttaa caaaaacttt tctcttttta ggatttagtt
     9961 ttacagaccc taatttagaa agaattttat cagatataag atgggttttg agagaaaacc
    10021 aaagacctca ttattgtata atgagaaaaa tattatagaa aaaattttag gttatcgctg
    10081 aaagccccgg aaatacgggc atttcgcagg atatgagaac tcaatttact accaatttac
    10141 tactttcgga ttgagccgca tatatgctga accatactgc taatacccgc caccccaaat
    10201 acggcacatc ataaattgaa tacgacaccg taaacgcggc gttcctcttt cccgtcatgg
    10261 gagaacagga cgccgctttt ttatgcccaa atagagaaag gaggcgacgc catgctattt
    10321 acttccggcc ctgaccgggc gtttgaacgg ctgatgcagg aaaagcccgg tttcgatcac
    10381 tacgacggcg gcggggcgga cgcgccggag gattgcggca cctgccgatt ttaccgcccg
    10441 gattggaaat atcaattctg ccagtttgca gagtgtccct atcagccggg caagctgacc
    10501 gactatgcag cggtcaaatt cacagtgaaa ggagcgaaca acgctatggc agttttccgt
    10561 gtggaaaaga acaaaggcta tacggtgatg tcaaatcacc acctgcggaa taagaacctg
    10621 accttaaagg ccaagggcct gctgtcgcag atgttgtcct tgccggagga ttgggactat
    10681 accctaaaag gactgtccta tatcaaccgg gaaagcatcg acgcgatcag gacagcggta
    10741 tgggagcttg aaaaagctgg atatatccgg cgtgagcagg gccgtgacgc aaaaggcaaa
    10801 atggccgata tggtctatac catctacgaa cagccggtat tggaaaaccc ggtattggaa
    10861 aatccaacat cggataaccc ggtgttggaa aatccgacat cggataatcc ggcgtcggaa
    10921 aatccaacgc aattaaataa agaagtacaa agaactgact tatcaaaaaa agaaaaaata
    10981 attacagatg gacaaagtac ccattccatt cctatcccat ccccgacccc tgcccctatg
    11041 gagggcgagg cagcggctac gccgccggaa cggaaaagga caggaaagga tgaggctgta
    11101 cggatttatc gggaaatcat tttggagaac atcgagtatg cctatctcat tcaggacagg
    11161 agcattgacc gtgaacagct tgacgaaatc gtagacctga tgctggaaac cgtctgcacc
    11221 gcccgaaaga caatccgtgt cgccggggac gactaccctg ccgagctggt gaaatccaag
    11281 tacatgaagc tgaccggcga acatatccgc tttgtccttg actgtctgcg ggagaacaca
    11341 acgaaagtcc gcaatatcaa acaatacctg cgggctatgc tgttcaacgc ccccagcacc
    11401 atttccaact actacacggc ccttgtcgcg cacgacatgg cacagcccga ttggggaaag
    11461 cctaaatccg gcctgccgga ctattcctgc ggcgagggcg aaagcctgta acgagaaaag
    11521 gaggacgacc ctatgaagca gggcgcattg atttttgacg agcgaacaga ccgttacgac
    11581 atccgctttg atctggccga ctattacggc ggcctgcatt gcggggagtg tttcgacgtg
    11641 atgatcggcg gcaaatggag gcccacccgc attgaaatgg ccgaaaactg gtatcttgtc
    11701 ggcatccggg ccgacgacct ttccggcctg cgggtgcgga tttgacctat atctacctgc
    11761 ggcgtctggt ggttccgcct tagacgccgc ttttgacttt ctgacaagga gggatgcgat
    11821 tgcaggaaga agttactcaa aagaccattg ccctgtccat gaaaacgggc aagctcacgg
    11881 ctcaagtgct gcaagcggcg ctgaaaaagt acctgcaaca ccgggccaag ggcaaaaaca
    11941 cgctgcacca cggccagcag agcctaaagc agcttaaaaa gcatggggct gatctctcca
    12001 acattgagat caccgaggcc aacattggag cgttcaagcc atgcgccaag aaatacggcg
    12061 tggattttac cttacgaaaa gataagacca cccagccgcc ccactacgtt gtgattttca
    12121 aggccaagga tgcagataac cttgatctgg cgtttcggga gtttaccgcc aagacgcttt
    12181 ccaaggagca gcggccctct atccgcaagg ttttgtccgc tgtgaagcaa aaggcggcgg
    12241 cccagaccaa gcagcgggcc aaggagaaaa tcaaggaaag ggggctggaa ctgtgaagcc
    12301 tgacgtaaag aaattgctga tcctgaacct cccgtatctg atttttgtgt acctgtttgc
    12361 gaaatgcggg gaggcgtacc ggctggccgc tggtgctgac ctgtcgggga agctcctgca
    12421 ttttgcggat ggttttgcgg cggcttttgc caaccctctg ccaagcctgc acctgtttga
    12481 cctgtgtatc ggtatcgccg gtgccgtgat cgtgcggctg gtggtgtact gcaagagcaa
    12541 gaacgtgaag aaataccgca aaggcatgga gtatggcagc gcccgctggg ggacggccaa
    12601 agacatagcg ccttacatcg accccaagtt tgaaaataat atcctgctca cccaaacgga
    12661 acggcttacc atgaccggca ggcccaaaga ccccaagaca gcgcggaata aaaacgtgct
    12721 ggtgatcggt ggcagcggtt cagggaaaac gagatttttc gtgaaaccca acctgatgca
    12781 atgctttccg accacggact atccgacttc gtttgtggtg acagatccga aaggcacttt
    12841 ggttctggaa accggcagga tgttccagca ggcgggctac cgcattaaaa tcctgaatac
    12901 cattaacttt tccaagtcca tgaagtacaa cccctttgtc tacatccatt cggaaaagga
    12961 cgtgctaaag ctggtaaata ccctgatttc caacaccaag ggcgaggggg aaaagtcagc
    13021 ggaggatttt tgggtgaagt cggaacggtt gttttattcg gccctgatcg gctatatctg
    13081 gtacgaggcc ccagcggagg aaatgaactt ctcaacgctg ctggaaatga taaacgccag
    13141 tgaagcccgc gaggacgacc cggagtttca aagccccgtc gatctcatgt ttgagcgttt
    13201 ggagcagaaa gaccccgacc attttgcggt gcgccagtac aagaaattcc tgctgtccgc
    13261 aggcaagacc cgttcttcca tcctgatttc atgcggtgcc agacttgcgc cctttgacat
    13321 ccgggaggta cgcgagctga tggaggacga cgaaatggag ctggacacca tcggggacga
    13381 aaagacggtg ctgtatctca ttatgagcga tacggacacg acctttaatt tcattctggc
    13441 tatgctccaa agccagctaa tcaacctgct ttgtgaccgg gctgatgatg tgtacggcgg
    13501 gcggctgcct gtccatgtac gcctgatttt ggatgaattt gccaacattg gccaaatacc
    13561 caatttcgat aaactcattg ccaccattcg aagccgtgaa atatcggcct ctattatttt
    13621 gcagagccag tcgcagctaa aagcgattta caaggacgcc gccgagatca tttcggataa
    13681 ctgcgatagt gtgcttttcc ttagtgggcg cggcaaaaat gccaaggaga tttccgatgc
    13741 gctggggaaa gaaacgattg acagtttcaa caccagcgag aaccggggaa gccaaacgtc
    13801 ccacggattg aactatcaaa aattaggaaa ggagttgatg tcccaagacg aaattgccgt
    13861 gatggacggc ggcaagtgta tcttgcagct acggggtgtg cgcccgtttt tatcggagaa
    13921 attcgatatt accaagcatc cccggtacaa ataccttgcc gacgccgaca agaagaatac
    13981 ctttgacgtg gacagatttt tgactatcac gcgccggaaa cggcagcaag tggtaacgca
    14041 ggatgaagta ttcgacctgt atgaaatcga cctgtcggac gaggacgccg ccgctgaata
    14101 aacagcggct tttttcattc caacaacaaa atcaaaaaat cattatgagc cggaaaccgg
    14161 cagaaagagg taaatttatg gcatttttta attccgcagt tactactttg cagactatcg
    14221 tgatcggact gggcggcgct ctgtgcgtat ggggcggcat caatctgttg gagggatacg
    14281 ggcaggacaa tccgggggca aaatcgcagg gtgtcaagca gcttgtcgct ggcggcggcg
    14341 tggccctgat cggtgtcact ctggttcccc tgctgtccgg gctgctgggc tgatcctggc
    14401 ctttgtccct gtccaaaccc ttaactaatc gcctccgcgt cctcccggcc agaccgggag
    14461 ggcgcgggaa aggaggtgct acccgtgatt agtgaaatca ttgaagaatg gataaaaggc
    14521 atcctcatgg acggtatcac gggcaacctg tccgggctgt tcgatacggt aaatgccaaa
    14581 gtcggagaaa tcgccgccga cgtgggacga accccgcagg catggaacag cggtattttc
    14641 aatatgctgc acagcctgtc ggaaaccgtg atcgtcccca tagccggggc catactcgcc
    14701 cttgtcatgt gctatgagct tatccagatg ataacggaaa agaacaatat gcacgacttt
    14761 gacagctcca tgttcttccg ctggatattc aaatccgcgt ttgcaatcct gatcgtaacg
    14821 aacacatgga acatcgtcat gggcgtattc gacgccaccc aaagcgtcgt caatcaaagc
    14881 gccggtgtga ttatcgggca gacatccatt gattttaaca ccctgatacc agaccttgaa
    14941 agccaattgg aggctatgag cgtgggcgct cttttggggc tgtggttcca aacccttgtt
    15001 gtgggcctca ccatgaatat cctttccatc tgcattttcc ttgtgaccta tggccgcatg
    15061 gtggaaatct atgttgtcac gtcgctgggg cctatccccc ttgccacaat gggcaacgcg
    15121 gaatggcgcg gcatgggcca agggtatctg aaatccctgc tggcgctggg cttccaagcc
    15181 tttttaatca tggttgtggt gggtatctac gcggtgctga tacagaatat cggttccgct
    15241 tccgacatat ccggcgcaat ctggggctgt atgggctata cggtgctgct gtgcttctgc
    15301 ctgttcaaga ccggcagtat atcaaaggcc gtattcgggg cgcattgatc ccaaaatcca
    15361 agcatacaca ggaggaactt gtttatttca acgtacaaca tcatttacgc tgacccgccg
    15421 tggcggtatg actgcaaaaa cgtacagggc gcagcggaaa atcactattc caccatgagc
    15481 attgacgagc tgtgcgccct gccggtggaa cggctggcgt ccaaagactg cctgctgttt
    15541 ttgtgggcca cattccccat gctgccggaa gctctgcggt taatcaaagc gtggggcttt
    15601 tcattcaaga ccgtggcttt tgtatggctg aaacagaacc gtaaaagcct tacatggttt
    15661 tacgggctgg gccgctggac aaggggaaac gcggaaatct gcctgctggc aacacacggc
    15721 aagccgaagc gtcgttctgc ctctgtccat cagtttatta tcagcccgat tgaacaacac
    15781 agcaaaaagc cggatgtgac ccgtgaaaaa atcgtagagc tggcgggcga tttgccccgc
    15841 gtggagctgt ttgccagaca gaaaaccccc ggctgggacg tgtgggggaa cgaggtggaa
    15901 tgtgacctta tcctgacgtg aaaggaggtg ccaaatggct tatgtaaccg tccccaagga
    15961 cttaacccat gtgaaaagca aggttgtgtt tgggcttacc aaacggcaac tgatctgttt
    16021 ttccggggcg ctggtgatcg gcgtcccctt atattttctc acccgtgact atcttaccaa
    16081 cagcgcggcg gccctgctca tggtatttgc catgctgccg ggctttttgc ttgccatgta
    16141 cgagcgtcac ggccagcccc ttgaagtggt gattatgcag atcatccagt gctgcttcat
    16201 ccagccaaag gagcgtccct atcagaccga caacacctac accgcccttg tgcggcaata
    16261 ccagatggaa caggaggtaa aggccattgt ccaaaaagga aatcccaaga gaaaccgaaa
    16321 aaacaaagct gacccgcgcc cagcgaaaag aaattgacgc ggtaatccgt aaatataagg
    16381 gcgacggcag gccccacacg gcgcagcaga gcatccccta cgaggtcatg tacccggacg
    16441 gcgtgtgccg ggtatcgccc ggcgtctttt ccaagtgcat tgagtttgcg gacatcagct
    16501 atcagcttgc ccagccggac acgcagacgg ctatctttga aaaactgtgc gacctgtata
    16561 actatgtgga cgcctccatt catatccagt tttctttcct caatcggaaa gttgacccgg
    16621 tgcagtacgc caagagcttt gagatcgcgc cgcaggggga cgacttcgac gacatccggg
    16681 ccgagtacac cggcattctg caaaagcagc ttgcaaacgg caacaacggc atggtaaaga
    16741 ccaaatacct gacattcacc atcgaggccg aaagcgtgaa agcggcgcga gccagattga
    16801 agcgcatcgg ctttgacctg ttaggctatt tcaagagcat gggggccgta gcccatgtga
    16861 tggacggctg ggaacggctg aacctgctgc atggcgtgta ccacccggac ggcgagatat
    16921 tcaattttga ctggaagtgg ctggccccgt ccggcctctc caccaaggat tttattgccc
    16981 cgtcctccct gtgcttcggc aatgcaaaga cattcggcat gggcggcaaa tacggcgcgg
    17041 taagtttcct gcaaatcctg tccccggagc tgtcggacga tatgctggcc gacttcctca
    17101 atacggaaag cggtgtttta gtcaatctcc atgtgcaggc cattgaacag accaaggcga
    17161 tcaagaccat caagcgcaaa attaccgacc ttgacgctat gaagattgcg gagcagaaaa
    17221 aggccgtccg cagcggttac gatatggaca ttcttcccag cgaccttgcc acctacggcg
    17281 aggacgccaa gaagctgctg acaaagttgc agacccgcaa tgaacggctg ttccagttga
    17341 cgttccttgt cctcaacgtg gccgacacca agcaaaagct gaataacgac gtgttccaag
    17401 ccgcaggcgt ggcccaaaag cataactgtc cccttgtccg gctggactac cagcaggaac
    17461 agggccttgc aagcagcctg ccgctgggcg tcaatcagat caagatacag cgcagcctta
    17521 cgacttccag cgtggccgtg ttcgtcccct ttgtgacgca ggagcttttt cagggcggcg
    17581 cggccatgta ttacggcatc aacgccaagt cccgcaacat gattatgctg gacaggaagc
    17641 aggcccgttg ccccaatgcc ttgaagctcg gcacccccgg cagcggcaaa tccatgagct
    17701 gtaaatccga gatcgtcagt gtgttcttaa cgacgcctga cgatattttt atttcagacc
    17761 cggaggccga atattacccc cttgtcaaac ggctccacgg acaggtaatc cggctgtcgc
    17821 ctaccagcaa ggactttgtg aacccgctgg acatcaacct gaattattcg gaggacgata
    17881 acccgctggc cctgaaatcc gactttgtgc tgtcgttctg tgagcttgtc atgggcggca
    17941 agaacgggct ggaagccatt gaaaagaccg tgatagaccg ggctgtgcgc gtgatctacc
    18001 ggccctatct ggcagacccc cgcccggaga atatgccgat cctgtccgac ctccacaaag
    18061 cgttgttaga tcagcatgtc ccggaggctg accgggtggc gcaggccctt gacctgtatg
    18121 tgtccggctc cctgaacgta tttaaccacc gtaccaacgt ggacatcggc aaccggcttg
    18181 tgtcctttga cattaaggag ctgggcaagc agctcaaaaa gctggggatg ctcattgtcc
    18241 aagaccagat atgggggcgc gtcacggcca accgaagcca aggaaaggcc acatggtatt
    18301 ttgccgacga gttccatttg cttttgaaag aggaacagac ggcggcctat tctgcggaga
    18361 tttggaagcg gtttaggaaa tggggcggca ttccaacagg tgccacccag aacgtcaaag
    18421 acctgctttc ctccccggaa attgaaaata tccttgaaaa cagcgacttc attacgctgc
    18481 tcaatcaggc gtccggggat agaaaaatcc ttgcggaacg cttgaatttg tccaccgaac
    18541 agcagaagta catcgacaat tccgaacccg gcgagggcct tttgatcttt gaaaacgtgg
    18601 tgctgccctt taccaacccc atcccgcaca acacccagct ttacaagatt atgacgaccc
    18661 gcttaaacga ggtggcggga gtatgacccg gcgacgccac aaaggaggtg aaccaccatg
    18721 aaagagctga aagccaaggc gaaagtgacg caggccatga cccgcgacgg gctgacgctg
    18781 gaaaatcagg ccaccggcga ggtggagaac atttccagcc gggaggccga acaggatttg
    18841 tccgcgccgg ggccgggcgg caccgccgat aagctgctgg aacgggccga cgccatccat
    18901 gacggccata aggccaagaa agccgcgaag caggccgggg ctgtggccgc cgagggggac
    18961 gctgccctgc accggccatc cgcccggctc caactgtcgg aggaagaacg ggccgacccg
    19021 gatttacagc cctatatccg aaaatcggac aaacgggccg ataagctgga taaggcccgc
    19081 gccgccatcc ccaagaagcg cgtcccggtc aaggaaaccg tctttgacga ggcaagcggc
    19141 aaagcaaaga ccgtcctgca ctttgacaaa caggacaagg ggcctccctc cctgaaaccc
    19201 aatccggcag gccgtcctgt ttccgaggtt tttctattcg gccacgggaa aatccatgaa
    19261 gtcgagcatg aaaacgtggg tgtggagggc ggccataagg gggaggaact tgccgagcgg
    19321 caggccggaa aagccatccg cagcaccatc cggcaccaca ggctgaaacc ctactgggac
    19381 gcggaaaagg ccgagcgcag gctattgtct gccaacgcgg agtatttcta ccagaaatct
    19441 ttgcgggata acccggagat cgcacaggcg gccagcaacc ccatttcccg gttctggcag
    19501 aaacagcaga tcaagcgcca gtacgccaag gccgcccgga cagcgggcca gaccgcaggc
    19561 gcggcggcac agggcgcggc caagacagcc ggggcctcca cgaaagcggc caaggccgca
    19621 gccaaagaaa gtgaacgggc ggcctccttt gtggcccgcc actggaaagg ggcgctggcc
    19681 gtccttgtgt tggggctttt gatgctgctt atcatcggcg gcttacagtc ctgcaccgcc
    19741 atgttcggca gcgccggaag cggcatcacg gcgtcctctt acttttccga ggatagcgat
    19801 atgttggccg ccgaggacgc ctacgcggag ctggaagccg agctgcaaga ctatctggac
    19861 aactacgagg ccacccatga ctatgacgaa taccattttg accttgacga gatcagccat
    19921 gacccctatg tgctgatttc cattctgtcg gcgctccatg acgggatatt taccatcggg
    19981 gaggtacagg gtgatttgtc catgctcttt gacaagcaat atatcctgac agaaaacgtc
    20041 gtggtagaaa cccgttaccg caccgaaacc gatacatgga cggacgcgga gggcaacacc
    20101 catacagaca cctaccaagt gccgtatgac tattacatct gctacgtcac gctggaaaac
    20161 ttcgacctgt cccatgtgcc agtctatatc atggacgagg aaacgctaag tttctattcc
    20221 ctgtatatgt ccacgctagg aaaccggcct gatctgtttt ccggcagcca gtaccccaac
    20281 gcctccaccc tgacgccgcc gacctactac gacgtacccc cggaggcttt ggaggatgcc
    20341 acctttgccg ccatgctcaa agaggcggaa aaatatatcg gttatcccta tgtgtggggc
    20401 ggcagctctc ccagcaccag ctttgattgc agcggcttca tttcgtgggt aatcaatcat
    20461 agtggatgga acgtgggacg gctgggggcg cagggcctat ataacatctg cacccctgtt
    20521 tccagttcgc aggccaagcc cggcgacctt gtatttttca agggaaccta cgacaccccc
    20581 ggcgtgtccc acgtcggcct gtatgtgggc aacaatgtga tgctgcattg cgggaacccg
    20641 atttcctaca cgaaccttac ttcaaactac tggcaatccc atttttacag ttacgggcgt
    20701 ttaccttaac gccagaaagg agctttcaat tatggcaacc aaacttgacc gcatcgaaaa
    20761 ggacatccag aaaaccaagt ctaagatcgc ggagtttcag aaacagcttc gggagctgga
    20821 aacgcagaaa accgaacagg agaatttgca gatcatccag cttgtgcgcg gcatgaacat
    20881 gaagccggag gaatttgccg cgttcttacg cagcggcgca atgcaggccg cccctgccgc
    20941 aaccccttac catgaacagg aggacaatgc ccatgaagaa taagaccaga cggatttccc
    21001 gtattctccc cgcacttctg gccgtgatgc tctgcgtcac ggcctttccc gttaccgccc
    21061 ttgcaggcgg caatgacccg gcaccggccc cgctgccgga ggctacaacc gaacccgcta
    21121 cgggcggttt ggagcctgaa acagacgaaa ccggcaccgc cacggacggg acagaaaccg
    21181 aggccgaaaa agataccggc cctctgaccg gcaaggacat caccgacctg ttttccgcgc
    21241 tgttcggttc caaggtgagc attgccgcca cggacgacgg catccagatc accaccggca
    21301 ccgacaaaaa agaacctgaa cagaccggca ccgtcaccac caacggcggc aggctgaatg
    21361 tccgcaccgg cgcggggctt gacaagaccg cgttcaccca gcttcccaat gggacgacgg
    21421 ttgaggtagt cggcacagac ggcgactgga ttaaaattct cttgccggag cgcatcggct
    21481 atgtccattc cgactatatg accgtcagcg aaaaggaagt agcggctacc ggcgagggcg
    21541 gtttttccct ctccattgac ccggaggaaa ttgcttctct gctggaactg ttcaatggcg
    21601 gcggggccag cgccgccctg tccccggacg ggaacctgtc actgattgac gacatcggca
    21661 gcgccgcgaa aaatggcaag cagtttatca ccgtggaaac caaaaacggc aatgtgtttt
    21721 atctgattat tgaccgggac gacgagggaa aagaaactgt ccatttctta aatcaggtgg
    21781 atgaagccga tctgctgacg ctgatggagg acggggaaac cgtcaccccc gctgctgtct
    21841 gctcctgtac cacgaaatgc aaggccgggg ctgtgaacac gaattgcccg gtatgtaaaa
    21901 ccaacctgac cgagtgcagc ggcccggagc cgcaggaacc ccagccggag gaacccgaag
    21961 ccccggagga agaacccaag ggcggcgcgg gcggcctgat cgtgttcctg ctggtggcgc
    22021 tggcgggcgg cggtgcggcc ctttactatt tcaagttcaa aaagcccaag gctgaaacca
    22081 ccggcagcga cgatttgggc gagtatgatt ttggggagga cgaggatttg gaggacgagg
    22141 aacccgaaac cgagtttgac ccggaagctg attttgtgag tgaacaggag gacgaggaat
    22201 gacatataaa cttgtaatcg cggaaaaacc cagcgtggcc cgcagcattg cgggcgtgat
    22261 cggggccacg gagaaacacg acggctattt ggaggggaac ggctatctgg tgagctggtg
    22321 catcggacat ctggtgtcct ttgccgacgc cggacgctat gacgagcgtt tcaaaaaatg
    22381 gcgctatgag gatttgccca ttctcccgga aacatggcag tacatcgtcc ccgacgacaa
    22441 gaaacagcag tttgacgttc tctgttcgct gatgcagcgc cccgacgtga ccggccttgt
    22501 gtgcgccacc gacgccgggc gcgaggggga attgattttc cgtttcgtct accagatggc
    22561 gggatgccaa aagccctttg agcgcctttg gatttcctct atggaggaca gtgctattaa
    22621 ggacggcttt gcccacctga aaccgggcgg cgactatgat ccgctgtatc aatcggccct
    22681 gtgccgggcc aaggccgact ggctgatcgg cattaacgcc acccggcttt tttccgtcct
    22741 gtaccacaag aacctgactg tgggccgcgt ccagacgccg accttaaaaa tgctggtgga
    22801 cagggacgcc aagattaccg atttcaagaa agagaaatat cacatcgtcc atatcacggc
    22861 gggcggcgcg gacgcggtaa gcagccgctt ttccgacgct gccgaggcaa acaccgtcaa
    22921 ggcggcttgt gcaaaagcgc aggccgtttg cgtttccgtg acgcgggaga aaaagacgga
    22981 acagccgccc aagctctatg accttaccac cttgcagcgg gaggctaacc ggcttttcgg
    23041 ctataccgcc aagcagaccc ttgactatgc ccaggcactg tatctaaaga agctgctgac
    23101 ctatccgaga acggatagtc aatatcttac cgacgatatg ctgcccgccg tggaaagcct
    23161 tgtgtccggc ctgtgggagc tggtgccgtt tgccaagggc ctgaaccttt ccccgcagtt
    23221 tgaccgcatc ctgaacagca aaaaggtgtc cgaccaccac gccattatcc ccaccgctga
    23281 atttgtgaag cagggctttg gcactctggc cgagagtgaa aaaaagctgc tgtccctgat
    23341 ctgctgcaag ctcttatgtg ccgtggccgg gccgcatatc tatgaggccg tcaccgccac
    23401 atttacctgt gccgggcagg agtttaccgc caagggcaag accgtccttt cccccggctg
    23461 gaaagagatt gaacgccggt tccgttcttc cttaaagacc gacgccgacg aggacgccga
    23521 ggcggtgcgg gagctgccgg agctttgcga gggccagacc tttgcagacg tggccgcctc
    23581 tgtcaccgag catttcacca cgccgcccaa gccctacacc gaagatacgc ttttgtccgc
    23641 tatggaacgg gccggggccg aggatatgcc ggaggatgcc gagcgcaagg gattaggcac
    23701 accggccacc cgcgccgcca ttctggaaaa gctggtgcag atgggctttg tccagagaaa
    23761 aggcaagcag ctcattccca ccaaggacgg catcaatctg gctgtggtgc tgccggaggc
    23821 gcttacttct ccccagctta ctgccgaatg ggaaaaccgg ctgaccgaga tcgccaaggg
    23881 gcaggccgat cctgacgaat ttatggacgg catcgagacg caggcgcggg agctggtgca
    23941 gacctattcc tgtatcagtg aggaaaagca gaaattgttt caggccgagc gtgttcctat
    24001 cgggacgtgt ccccgctgtg gtgaggctgt ctatgagggc aagaaaaact actactgcgg
    24061 aaaccgggcc tgccagttcg tcatgtggaa gaatgaccga ttttttgagg aacgcaagaa
    24121 agcgttcacc ccgaagatcg ccgctgcgct gctcaaaacc ggcaaagtca aggtaaaggg
    24181 cctctattcc gtaaagaccg gcaaaaccta cgacgggacg gtgcttttgg ctgataccgg
    24241 gggcaagtac gtcaactacc gcattgaaca caggagctaa aggacagtaa cacagcaagc
    24301 atagggagtg tggctacact cccggtaaaa tcccccggcc gcgtccctgc cgggccgggg
    24361 atttttgctt gttggggaag tccccaaccc cctatttttt gaaacagaac agagaaagga
    24421 gtgaccgcgt gaacgaagta ttttctatcc aagtccataa ccggcagttt ttggaacagg
    24481 gcaaacaagg cgtctggctg gatttgccca ccacaaccga aacgctgcaa gccgccctgc
    24541 gggatgttgg cattactgcc gacaatccgc aggatttttt tatcaacggg tattcctgcc
    24601 cggaggacag acaccttgcc attccctatg acatggtgct ggccgccgac gtggacgagc
    24661 tgaatttcct tgccgcccgg ctgggccagc ttgacgccgc cgagatcgca gagctgaacg
    24721 ccgccctgca aaatccccgt ggcggctttg agaatatcgg ccagatcatc gactacaccg
    24781 aaaatgtgga ttactatgtg catcttcccg acgttagggg ggatggacag ttgggcgact
    24841 actacctgaa ccgttccggc atggtggata tgccggagga atggaaagcc gggattgata
    24901 cggcgcagtt tggacgccac attgcccagc aggaacaagg cgtttttacc cagtacggct
    24961 atctggtgag aagcggcgac gaatggcagc gcgtccatga gggccagccg gtgccggagg
    25021 aataccgggt gatgcactac ccccagccgg aagctgaccg ggaggaaccg caggcccagc
    25081 agccggaggc atccgaaagc cccgacaaag cgccgccccc ggttatcccg atcatgctgg
    25141 acggcatgaa cagcaaagaa cgcatgaaag tgattactga ccgtctggaa accggcgtac
    25201 aggagctttt tgaaagtagc cgctatatgg cttacttatc cgctatgtcc aagatgcacg
    25261 attacagtct gaacaatacg ctgctgatcg ccatgcaggg cggttcgatt gtggcgggct
    25321 ataacaggtg gcaggatgaa tttgaccgcc atgtgaagcg gggcgagaaa ggcattaaga
    25381 tactggcccc ggccccgttc aaggttaagc agcagatgga gaaaaccgac ccggccaccc
    25441 agcagcccgt gatcggcgcg gacggcaagc ccgtgaccga agaacaggaa gtgacggttc
    25501 cggcctttaa ggtggtgaca gtctttgaca tcagccagac cgagggcaag gattttccca
    25561 tgccctatat cagtgaactt accggcgacg tgcagcagta tcaggatttc tttgccgcgc
    25621 tggaacggac atcaccctat cccataagca ttgagccgat ttcagacggg gccaagggcc
    25681 gctgcttcta cgcaaaacag cgcattgcca tcaacgaggg catgagcgaa ttgcagaccc
    25741 taaagaccgc catccatgag attgcccatg ccaagctcca cgccattgac cgggaggccc
    25801 ccgccgagga acaggcggcc cggccagacc gcagcacccg cgaggtacag gccgaaagcg
    25861 tggcctatac cgtctgccag tattacgggc tggacacgtc ggactattct tttggctatg
    25921 tggccgattg gagcagcggc agggagttat ccgagctgaa agcctccctt gaaaccatcc
    25981 gggccacggc cagcgaattg ataaccgaga tcgacggcca ttttgcccag cttcagcagg
    26041 agcgcgaggc ccggcaggaa caggaggccg tcacggttga cgttaccccg gaggcagcgg
    26101 ccccggcgca ggccgaggaa gaacaggccc cggcccttga tccggcagcc gagccgcttg
    26161 tgactgtgct atggagcgaa agcccccagc taaaggacgg ccagcaaatg ccgttgcatg
    26221 aggccgacgc gctgtttgcc cgtctggatg cagcatatcc ggcagaggca ggctatgaca
    26281 aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc ggacagctac gaggggcggc
    26341 aggattttgg agatagggac ggttccctga tcgaacatat ccagagctac caccaatttt
    26401 acgcgcagga cgaaaactgg aaaaagcatg taatcgctac aaccggcata gagggctggg
    26461 agcaggacaa ggcccatcgg gatatgctgt taggcgagtt tgtcccctat atgaagctgc
    26521 attgcagcct tgcccaaatg gaacagacgg cgcagggccg cttacagtcc ggggaaaccc
    26581 tgacgccgga ggaaacggcc tatttggggg cgcttctctc ttatgtgcag gagtgccgcc
    26641 ccatgctcaa tcagggccag tatcagttgc cggagccgcc gaaactggct gacttcgacc
    26701 agagcttgca ggactacaaa gcacaggtac aggccgagat cgcgcaggag gccgccgcag
    26761 ccggtatgac cgtggaggaa tacgcggcgg ctggctatga agcaccccaa caggacacct
    26821 tttccatcta ccagcttcga ggcggggagg aaacccgcga ctatcggttt gagccatacg
    26881 agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt gtttatacgg
    26941 caccgctgga taaaagcacg tctttggagg atatttaccg cacattcaac atcgaccacc
    27001 cggcagattt taggggacac agcctttccg tttccgacgt ggtggtgctg caccagaatg
    27061 gacaggacac cgcccactat gtagacagct tcggctatat ggaagtgccg gagtttttgc
    27121 gggaaaatcc cctgcgtacc gccgaactgt ctacggagca gaacgaaaac atgattgacg
    27181 gcattatcaa caatacgccg cccgaaccgc agccggagga acccgaacag ctatcttttg
    27241 aggatgtggc agttgcttcc ggccagacac aggaggaacc cccggaggct gcgcccccgc
    27301 aaacggctgt ccgctattat cccatcaatg agggagccgc ccgccgcgcc aaggaagcca
    27361 tgagcttttc tgactaccgg cccggcagcg ccacggcgga ataccggcac tatgtcgatc
    27421 aggccgtgga aattgcggag cgccagaaaa agcgtgtgga gccagagtat cacgaaaaga
    27481 tagacagcct ccttgacacc tacgcccgca agctggccga gaacatgaat aagggctatg
    27541 agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc ccggtacggg
    27601 cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga ggaatttcag tacattcagg
    27661 gcttgcttga caaaatccgc agcaccggca tgggtggtat cagcgccgac gatccgcagg
    27721 ccgtttccaa gctggaaaag aaactggaaa agctggaagc gtcgcaggag ttgatgaagg
    27781 ccgtcaatgc ctactaccgc aagcatggga cgctggacgg atgcccccat ctgatcgaaa
    27841 gaggcattga aaacctgaaa gcggatatgg cgtcgggctg gcactatgag aaaaaaccgt
    27901 tccagtcctg gcagctctcc aacaataacg ccgagatacg ccggttaaag ggccgcattg
    27961 aggaattgac ccgccaaaag gaggcggcct atgtgggatg ggagtttgac ggcggcactg
    28021 tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag ccggatgctg
    28081 ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc ggggcatggc
    28141 agcggcagtt aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc atccagcccc
    28201 tttccggcga aaagcccagc gccttacagc ttgcggcccg cagggagaaa gaaaagccct
    28261 ctatccgggc gcagctcaac gcggccaaga ccgaacaggc caagcagcca ccggcgcggg
    28321 aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg gacgttctgg
    28381 ccgcgctgga acagatcatg cggcagaaca ccgcgtttta tcaaagtgac tttggcattg
    28441 acaaagacat tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag accctgctct
    28501 ggatgtcccg gccctccggg acgtactgtt tccgggaacg tgatgttttc ctgaaagaca
    28561 cccgacagta caacacatgg aaattttacg gggagcagac ccgtgacaag atactggcct
    28621 acgcggtgga gctgaccggc acacagggcg gcactatccg gggcaatctc tatgagctgg
    28681 actatccgca gcatttccgg cacgtcattg aaaccgccca gccggtgagt gtgaaccggc
    28741 tcttttatga gcatggcacc cgtgacatcc cggagagcca gcgttttgac ggaagccctg
    28801 accgggtatt gggaaacttt ttgtactatg aggcccagcc ccatgacccg gctgtgctgc
    28861 aggaggcgct acggacggag cagcacagcc gggggcgggc cgtccccggc gatttcaaag
    28921 tccacgtcac cgctttgcat gacagccgga ttgaaaccga ggcccggcgt attgtggaca
    28981 gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac gcatttcatg gtggagatct
    29041 ccccgcactt tgcggcgatt gcttccacca aggataatga ccggctcttt gccatgctgc
    29101 cctataaatc cctgtgtttt accgggatga aaggcaggca tggcgtgtat gccgtgatta
    29161 accgggacga gcgccgggat gtgagtattc gcaggccccg gccctctatc cgcaagcagc
    29221 tatccgacag caaacaggcc gccagcccaa aaaaggcggc ggccagaacc aaaaagaatg
    29281 aattggaggt ataaggaatg atgcacttta ccgtggagga agaaaacctg atctgtatgt
    29341 accacaatgc agaccggcgc agaacgataa acaggctcac cgccgtcatg ccggttatgg
    29401 atggggatat gcgggcgcag gcccagcaga ccctttccaa gctggaacgc atgacggatg
    29461 ccgactatga cggccagaag ttccacttca cagacgaata acgaacagca gacgccgggc
    29521 gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt cctacgcatt
    29581 tagaacccgg tattgtcggg gataggatga agtattcgct tgcccctgca aacatgcggg
    29641 agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag ctataattag
    29701 acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg tgcaggtgat
    29761 agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt tccagcggga
    29821 agctcggcaa tgcttccagc agtttcaggg aaatgcgctg ccgtatatct tcgtcaatga
    29881 ctttcttctt tttgccgttt gcctgtctga tctccaccgt ggaaagctcg ttgatgtagt
    29941 cagcataatg ggccaacact ttttccgtgg cccacctctc accggcaacg gcggctttga
    30001 tcgtttcata gtccagcagc ttttcttgtc cgtccatcct gatacccccg tcagactgat
    30061 tttttctatg ataccatatt cggaagcatg gaaccagaga aaacggcgct tttctgctgg
    30121 taaattctac gcatttagaa cagcacttcg gcggggatag gataaaacac ccacataccc
    30181 cgctgaccat gccggaaaag ccttgaaata taagggcttt tacagctcta agtgcgtagg
    30241 tttccagtat cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc gtttggagca
    30301 ggccgcgcag tatttggccc ggttagactt cggcgtgaac gtggccccgc actcacagca
    30361 gcgtttcact tcgtcccggt ctttggtgat ctcggcatac agggccgcat ccagcgggag
    30421 gacagcggcc ttaaaccagc ggcacagaag cgaataggaa atgctctgta cgcaaataca
    30481 ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa tagttgcagc actcatgcgt
    30541 cagcttccgg gccttgcgat attgagggta attcattcgt ggaatactca tagctcttgc
    30601 gtccattcct ttcccggcgt catacgcaaa atgccgtcca cattctgctt gatgatgccg
    30661 tattcctgca tctgcttttt cgcttcgccg tacttctcat acagccgttc cttttcctcg
    30721 gccaatcgct tgtactcggc tttcagggcg tacagatcgg ggagcttttt cacaccctgt
    30781 ccctttaggg cctttgccgc agcttcatac aaaatcaacg tgctttcatg ctcacgccgg
    30841 aattttgcct tatccgctgc attgcggtat tccatagcca cgggccagag ctgcttgtag
    30901 gtagaaatat tctttatcag caccgccata tccgcaagcc ggtgttccat agctttgagg
    30961 gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca ctttgctttc caaatccggg
    31021 taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg cttcaaattg
    31081 tggagcttcg cccacctctc atagcctgcc gactgctggg ccttaatgtt gttttccaga
    31141 tcaatccgca aagaaatccc cttgcgctct ttgggcgctc tggtgataac gcggcctttg
    31201 atacgctctt tgatggcttc ctcggtgtag gcttccccca atgtcttgca gcgggtaaag
    31261 cgttcctgtc cgggagcgcg gaaagaaata aactttcccg gcttgatctc atagccctgc
    31321 gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc ggcgtcaatg
    31381 gctgctttga gcttcgcttt ccagctcgtc cctttccgct gggcgtccca ctcggcatag
    31441 ctcttgccct tactctggcc gggcttcacc acggacaatc cgttttcctt gcacagcctg
    31501 tcgctggtgc gccggatata ggcatagctc tgtttgttgg aaatgtactt gcgctgattt
    31561 accatatcca cggcacagaa aataatgtga ttgtggacgt ggcccttatc aatatgggta
    31621 gtcagcacat agggatattt cccttgcagc acttcctcgg caagctgctt cccgatctca
    31681 tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg gtggcccaga
    31741 ttattgccct tttggtatgc ctgcgccagc agcatttcaa attcaatgtc cgctgtttca
    31801 taagagcagg caaaggaggc caccagcagc ttgccgtcag ttttgtccgg gttctcgatg
    31861 tagtcaaggg cttttttcag cgttgacttg ataggatgta tctttgtaac tgccatatct
    31921 gcgccagcgc cccctttata tcctcgatgt cctgtgcgta caccggcccc ataccgttca
    31981 gcctctttgc aatctgattg acgttgacgc cgatcttctg tatctcggcg gtctgcgccc
    32041 ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat gagcatcttc cgggcgtagg
    32101 ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc aatcatagcc cgttcctccg
    32161 gcgtcacagg gacgcgcagc acgatttttc gctttctgtt tgccatgctt caccgtcctt
    32221 tccattcagg ggtttgggga tttccccaac aagcaaaacg gacgcggccc ggcagggacg
    32281 gggcaggccg tttttccggg agtggctaca ctccctatgc ttgctacgtt actgtttctc
    32341 cactaacaag acgtttccaa agccccaaac tacccgcaaa atagccctct ggaaagaaaa
    32401 aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa cagaggatgc
    32461 cagttacaca tcctccgttt cattcttctt cggcttttca attagtatgg cgctgtcaat
    32521 cgcgccctcg atgatcggca ggtattcttc ggggcagagt ttcagcttgt ggctgaccct
    32581 ttgccgcagt tcgctttcct cccgcatgat ctccgggtta aagtagcgtt ccacaggaag
    32641 cccgcaggct ttaataagct gtatgacaac cggcagactg ggaatggccc gtcgttttcg
    32701 atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt gcgcgaaacg
    32761 cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc gggacaatct
    32821 tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc tgcccgtgga
    32881 atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct tttattcttc
    32941 gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg acgttgaaag
    33001 aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt gctgcgggac
    33061 gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact gcggaaaagc
    33121 cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat gaaaccggcg
    33181 cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg aaaaactata
    33241 agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg aaacagaaag
    33301 aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga aatggaaaat
    33361 gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag aattgaaaag
    33421 gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc tggtctaatg
    33481 cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt ttgaggaaaa
    33541 tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg acacggatga
    33601 tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt attaggactg
    33661 aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg ccctgcctgt
    33721 gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt tgctgaggct
    33781 tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt ggatcagaat
    33841 atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt ttgacaaccg
    33901 ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct tgatttaatt
    33961 cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc ccaatgacgg
    34021 atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag actgttatgt
    34081 tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc gaggatgatg
    34141 atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag gtggatgcct
    34201 gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa ctggttattc
    34261 ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt cgtgcgaaaa
    34321 atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa atccgggcgt
    34381 ttgatgcaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt ttactaaagc
    34441 gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt gtcggcaggc
    34501 tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg cccctgacac
    34561 gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc ttaacgcatg
    34621 aaatcatttt gtcacgtata tggggatatg actttgaggg tgatggcagc acagtccaca
    34681 ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc attcgcggtg
    34741 taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca ttaaggtttt
    34801 ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac tgtttgcaca
    34861 gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat cctatcagcc
    34921 attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg cagggctgtt
    34981 tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa gcgtactcta
    35041 tgccacacca aatgccaata catcaaatag ttttagaccc aactttcttt atgtggtaca
    35101 tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat tgctttatca
    35161 agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca gccttttatg
    35221 cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg acagtgcgaa
    35281 taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg agcttggcgc
    35341 actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg caaggctgga
    35401 ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct ttgcggcagc
    35461 ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg gaatgcttga
    35521 aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca aaatgatgga
    35581 taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg atgggagaat
    35641 cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc tgcccgattt
    35701 tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag ccgggcaaat
    35761 tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat tgaatgcagt
    35821 tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg ctgaaaaatg
    35881 ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc caaggctgtt
    35941 caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa gcagaaaaag
    36001 tgggcgaagc ggtttaggac ttgccatcgt acaaaaaacg ctggatgcca tgagcctcca
    36061 gtatgcgctg gaaaacacct cggatggcgt tttgttctgg ctggatttac cgctcacatc
    36121 aacattgtaa atatttaaaa cttaaatgat tttgggctct ttgtcaaata ggactgatag
    36181 agtgagataa gaacatctaa ggtagaattc cttttctact ttagatgttt tttgattcat
    36241 aggtaatcag attatttagt aagaaaattc tggtcttcat tttccgaaag cttcgaaagc
    36301 cataagcgac tcgtttcaat gctttgatgt gggtatgcag attttctatt ttggcatttg
    36361 aataaggcaa ttcgagggct aaacgaatgc cggattcata gtttataaaa gcttttttta
    36421 tagaatgcag ttcttttgga acggaattgg gcatcgttct tacaagttta aagaaagtgt
    36481 ccgcctcttt gtcgtgaaac gcttgaagaa tttcttgata gtaaaaatag gcgattcgta
    36541 attcgtcaga gatcgaaagc agacgatcca ccacttcact ttcggtgaga tacgcccaac
    36601 caaaattgcg ccatttggtc catttgtcat aaacaagctt gtcctgtcgt ttgatcagaa
    36661 gccgagacaa cgcctttaat tgtcgataat atttggcttg tgttggatcg tttcgatcaa
    36721 agcttttcat gattcgtacg cgaatatcct caaatttacg aatgagatgt ttgacaatgt
    36781 gaaaacgatc cacaacgagt tttgcctttg gaaaacattc ctttatcaga gaggcataag
    36841 aaacattcat gtccgtcacg atgaccttta ccttcaatcg tgcgcgtcgt tcaaattgca
    36901 taaaataaga aacgagagcc tttttcttcc gtgaaggaag aatatccagt aattttccag
    36961 tatcgccatc catacaagaa aaggagtagg gaccgacttg atttttcagt gaacgaaatt
    37021 catccattaa taaacaacgc ggtaaccaat ttctttttac ttttgtcatg gggatcagag
    37081 aagcgagtgt ccgagaaacg gtcgaccaag aaacgccgca atgcttagca attgtcgtca
    37141 ttgcttgttt ttcagttagc tcttctaaaa ttttatagcg cacttgttta gcgataaagc
    37201 agtttctatc gacaatgtag gtattcgccg taaaataggt gtggcagcca cgacagtggt
    37261 agcgttgttt cgctaaagca agataagtca gttggttcgc acatttatta agttgaatga
    37321 gcgatttttt ctttccgttc ttaacgatca tgccttcatg attcatctgt ttacacaaag
    37381 gacagcgacg aatttcttta cttaacgtcg cagaaacaac aagactcttg atattcttaa
    37441 tgctcttcga ttccacattt gtgatgaaaa tgtttttatc tttgattcca agtagtagtt
    37501 tgatatgatg aagatcaggc atacagaacg ctcctttgat tgtgttgtgg tgattcaatt
    37561 ctaacggagc ttctgtatgt tttgtttttt catgctcaaa agggaccgat agaatgattt
    37621 tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc ctgccggtct
    37681 ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga tgcgcttgcc
    37741 ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc attcgatcaa
    37801 atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat gaaacaatcc
    37861 gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg cacgctgttt
    37921 ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat ccctgcaact
    37981 catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct tgccaccgcc
    38041 aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca gaaccccatc
    38101 cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat agatattcgg
    38161 atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt ttacccgatt
    38221 gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga aaaaattgcc
    38281 gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga aacttgggtg
    38341 gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa tgcggacgga
    38401 attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta tcgttttggt
    38461 tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa cgagccgtgg
    38521 cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg ggtttgcctt
    38581 gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac agaaaaagat
    38641 tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa atgtgctttt
    38701 atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa aaatcgcttt
    38761 cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga tttgacatga
    38821 tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta aacggcttat
    38881 tgataagacc gggggaaacc ttttctttct ggttgttcct gcatacgcca ttgacaatta
    38941 tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac gagattaaag
    39001 gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac gataccgact
    39061 gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag gtgttcgccg
    39121 acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat gtccgtgaaa
    39181 gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta ggtaccgggg
    39241 aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat cccctcccgg
    39301 aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta ctgtttttgg
    39361 atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc atattatccc
    39421 tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg gcaatcaatg
    39481 cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc tgagaaaggt
    39541 cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata cgactgccgc
    39601 cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg ttgcggatta
    39661 tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc acgccgtggc
    39721 gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata tgactgtggg
    39781 agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg gatttggatg
    39841 ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc agcttgatga
    39901 gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg atacccgcca
    39961 tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa tcaacaccgg
    40021 gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg gaaagctggg
    40081 tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg attgctcgca
    40141 gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg tgatcatcac
    40201 accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa aaacaatcag
    40261 gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc gcaatcatct
    40321 tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa attgctgcga
    40381 acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac ggtgtatgga
    40441 agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc atactctccc
    40501 cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa acacggcgta
    40561 ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg atacaggggc
    40621 tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc gcagcttgca
    40681 tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt cccgaatttc
    40741 aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct gtctttgtga
    40801 agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg gaagaactta
    40861 acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag caagcgattt
    40921 cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt gtcggcgaag
    40981 tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac gagccggaaa
    41041 aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag gaacgaaatc
    41101 gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg cttgcccgtg
    41161 ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat accctgcccg
    41221 gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc acgcttcctg
    41281 cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg aaaatggttt
    41341 tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg ccacatggga
    41401 taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg gcacaaaggc
    41461 cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct atggcttgct
    41521 tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt gggcagcgca
    41581 accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag ccgagttgat
    41641 tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa ttgatcttac
    41701 gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg attttatgga
    41761 cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa atcgaagatg
    41821 cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat ggtggcacta
    41881 taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt cataatgaaa
    41941 gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat ggacaatctt
    42001 ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt attcaatctg
    42061 cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc ccattttgta
    42121 ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca gcaaaaggtg
    42181 tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc taatccactt
    42241 ccaccactca aaaaaagcct tgcccctc
//
LOCUS       DAZAJZ010000012        41704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000012 DAZAJZ010000000
VERSION     DAZAJZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.012"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACG86D_000673"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACG86D_000673"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN3139847.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACG86D_000674"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACG86D_000674"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN3139848.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACG86D_000675"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACG86D_000675"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN3139849.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACG86D_000676"
     CDS             2179..2499
                     /locus_tag="ACG86D_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN3139850.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACG86D_000677"
     CDS             complement(2611..3468)
                     /locus_tag="ACG86D_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3139851.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACG86D_000678"
     CDS             complement(3576..4880)
                     /locus_tag="ACG86D_000678"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3139852.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACG86D_000679"
     CDS             complement(4877..5386)
                     /locus_tag="ACG86D_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139853.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACG86D_000680"
     CDS             complement(5561..7696)
                     /locus_tag="ACG86D_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN3139854.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACG86D_000681"
     CDS             complement(7779..9434)
                     /locus_tag="ACG86D_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3139855.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACG86D_000682"
     CDS             complement(9436..9750)
                     /locus_tag="ACG86D_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3139856.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACG86D_000683"
     CDS             9920..10180
                     /locus_tag="ACG86D_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN3139857.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACG86D_000684"
     CDS             10194..10508
                     /locus_tag="ACG86D_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN3139858.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACG86D_000685"
     CDS             complement(11238..11792)
                     /locus_tag="ACG86D_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN3139859.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACG86D_000686"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACG86D_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACG86D_000687"
     CDS             complement(14153..14869)
                     /locus_tag="ACG86D_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN3139860.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACG86D_000688"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACG86D_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN3139861.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACG86D_000689"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACG86D_000689"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN3139862.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACG86D_000690"
     CDS             complement(18339..19799)
                     /locus_tag="ACG86D_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139863.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACG86D_000691"
     CDS             complement(19789..21084)
                     /locus_tag="ACG86D_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3139864.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACG86D_000692"
     CDS             complement(21416..22093)
                     /locus_tag="ACG86D_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN3139865.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACG86D_000693"
     CDS             complement(22123..23214)
                     /locus_tag="ACG86D_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN3139866.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACG86D_000694"
     CDS             complement(23315..23749)
                     /locus_tag="ACG86D_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN3139867.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACG86D_000695"
     CDS             complement(24333..25790)
                     /locus_tag="ACG86D_000695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3139868.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACG86D_000696"
     CDS             complement(25787..27682)
                     /locus_tag="ACG86D_000696"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3139869.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACG86D_000697"
     CDS             complement(27740..29515)
                     /locus_tag="ACG86D_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN3139870.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACG86D_000698"
     CDS             complement(29565..31061)
                     /locus_tag="ACG86D_000698"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3139871.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACG86D_000699"
     CDS             complement(31255..32877)
                     /locus_tag="ACG86D_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN3139872.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACG86D_000700"
     CDS             complement(33205..34518)
                     /locus_tag="ACG86D_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN3139873.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACG86D_000701"
     CDS             complement(34763..34987)
                     /locus_tag="ACG86D_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139874.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACG86D_000702"
     CDS             complement(35007..35429)
                     /locus_tag="ACG86D_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3139875.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACG86D_000703"
     CDS             complement(35571..36281)
                     /locus_tag="ACG86D_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN3139876.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACG86D_000704"
     CDS             complement(36299..36796)
                     /locus_tag="ACG86D_000704"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139877.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACG86D_000705"
     CDS             complement(36833..37285)
                     /locus_tag="ACG86D_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3139878.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACG86D_000706"
     CDS             complement(37420..38025)
                     /locus_tag="ACG86D_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN3139879.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACG86D_000707"
     CDS             complement(38475..39095)
                     /locus_tag="ACG86D_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3139880.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACG86D_000708"
     CDS             complement(39097..39966)
                     /locus_tag="ACG86D_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN3139881.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACG86D_000709"
     CDS             complement(39989..40396)
                     /locus_tag="ACG86D_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN3139882.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACG86D_000710"
     CDS             complement(40415..41704)
                     /locus_tag="ACG86D_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3139883.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7021 g        13941 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccat
//
LOCUS       DAZAJZ010000013        40671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000013 DAZAJZ010000000
VERSION     DAZAJZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.013"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1185
                     /locus_tag="ACG86D_000711"
                     /pseudo
     CDS             44..1185
                     /locus_tag="ACG86D_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1311..2390)
                     /locus_tag="ACG86D_000712"
     CDS             complement(1311..2390)
                     /locus_tag="ACG86D_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3139884.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2493..2933)
                     /locus_tag="ACG86D_000713"
     CDS             complement(2493..2933)
                     /locus_tag="ACG86D_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3139885.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2990..3400)
                     /locus_tag="ACG86D_000714"
     CDS             complement(2990..3400)
                     /locus_tag="ACG86D_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN3139886.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3580..4014)
                     /locus_tag="ACG86D_000715"
     CDS             complement(3580..4014)
                     /locus_tag="ACG86D_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3139887.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4114..4962)
                     /locus_tag="ACG86D_000716"
     CDS             complement(4114..4962)
                     /locus_tag="ACG86D_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3139888.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5351..7117
                     /locus_tag="ACG86D_000717"
     CDS             5351..7117
                     /locus_tag="ACG86D_000717"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN3139889.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7219..8478
                     /locus_tag="ACG86D_000718"
     CDS             7219..8478
                     /locus_tag="ACG86D_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3139890.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8625..9926
                     /locus_tag="ACG86D_000719"
     CDS             8625..9926
                     /locus_tag="ACG86D_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3139891.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9929..10780
                     /locus_tag="ACG86D_000720"
     CDS             9929..10780
                     /locus_tag="ACG86D_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN3139892.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10793..11608
                     /locus_tag="ACG86D_000721"
     CDS             10793..11608
                     /locus_tag="ACG86D_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN3139893.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11759..12925
                     /locus_tag="ACG86D_000722"
     CDS             11759..12925
                     /locus_tag="ACG86D_000722"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3139894.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13155..13802
                     /locus_tag="ACG86D_000723"
     CDS             13155..13802
                     /locus_tag="ACG86D_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN3139895.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13886..14830
                     /locus_tag="ACG86D_000724"
     CDS             13886..14830
                     /locus_tag="ACG86D_000724"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN3139896.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14965..16416
                     /locus_tag="ACG86D_000725"
     CDS             14965..16416
                     /locus_tag="ACG86D_000725"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN3139897.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16437..17273
                     /locus_tag="ACG86D_000726"
     CDS             16437..17273
                     /locus_tag="ACG86D_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3139898.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17273..17932
                     /locus_tag="ACG86D_000727"
     CDS             17273..17932
                     /locus_tag="ACG86D_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3139899.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18058..18747
                     /locus_tag="ACG86D_000728"
     CDS             18058..18747
                     /locus_tag="ACG86D_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139900.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18753..19709
                     /locus_tag="ACG86D_000729"
     CDS             18753..19709
                     /locus_tag="ACG86D_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN3139901.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19753..20265
                     /locus_tag="ACG86D_000730"
     CDS             19753..20265
                     /locus_tag="ACG86D_000730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3139902.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20521..21069)
                     /locus_tag="ACG86D_000731"
     CDS             complement(20521..21069)
                     /locus_tag="ACG86D_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN3139903.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21158..22339)
                     /locus_tag="ACG86D_000732"
     CDS             complement(21158..22339)
                     /locus_tag="ACG86D_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN3139904.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22575..24083)
                     /locus_tag="ACG86D_000733"
     CDS             complement(22575..24083)
                     /locus_tag="ACG86D_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN3139905.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24242..24433
                     /locus_tag="ACG86D_000734"
     CDS             24242..24433
                     /locus_tag="ACG86D_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139906.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24636..25553
                     /locus_tag="ACG86D_000735"
     CDS             24636..25553
                     /locus_tag="ACG86D_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN3139907.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25546..26619
                     /locus_tag="ACG86D_000736"
     CDS             25546..26619
                     /locus_tag="ACG86D_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139908.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26767..27285
                     /locus_tag="ACG86D_000737"
     CDS             26767..27285
                     /locus_tag="ACG86D_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139909.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27266..27658
                     /locus_tag="ACG86D_000738"
     CDS             27266..27658
                     /locus_tag="ACG86D_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN3139910.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27734..28669
                     /locus_tag="ACG86D_000739"
     CDS             27734..28669
                     /locus_tag="ACG86D_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3139911.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28802..29581
                     /locus_tag="ACG86D_000740"
     CDS             28802..29581
                     /locus_tag="ACG86D_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN3139912.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29574..29891
                     /locus_tag="ACG86D_000741"
     CDS             29574..29891
                     /locus_tag="ACG86D_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN3139913.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30135..31745
                     /locus_tag="ACG86D_000742"
     CDS             30135..31745
                     /locus_tag="ACG86D_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3139914.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31902..32750
                     /locus_tag="ACG86D_000743"
     CDS             31902..32750
                     /locus_tag="ACG86D_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3139915.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            32981..33460
                     /locus_tag="ACG86D_000744"
     CDS             32981..33460
                     /locus_tag="ACG86D_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN3139916.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33493..35721
                     /locus_tag="ACG86D_000745"
     CDS             33493..35721
                     /locus_tag="ACG86D_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3139917.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35850..37493
                     /locus_tag="ACG86D_000746"
     CDS             35850..37493
                     /locus_tag="ACG86D_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN3139918.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(37566..38111)
                     /locus_tag="ACG86D_000747"
     CDS             complement(37566..38111)
                     /locus_tag="ACG86D_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN3139919.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38134..38619)
                     /locus_tag="ACG86D_000748"
     CDS             complement(38134..38619)
                     /locus_tag="ACG86D_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3139920.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(38609..39421)
                     /locus_tag="ACG86D_000749"
     CDS             complement(38609..39421)
                     /locus_tag="ACG86D_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN3139921.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(39560..>40671)
                     /locus_tag="ACG86D_000750"
     CDS             complement(39560..>40671)
                     /locus_tag="ACG86D_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN3139922.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13497 a         7378 c         7961 g        11835 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
      481 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
      541 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
      601 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
      661 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
      721 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      781 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
      841 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
      901 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
      961 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1021 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1081 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1141 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1201 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     1261 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     1321 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     1381 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     1441 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     1501 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     1561 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     1621 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     1681 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
     1741 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     1801 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     1861 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     1921 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     1981 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2041 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     2101 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     2161 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     2221 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     2281 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     2341 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     2401 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
     2461 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
     2521 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
     2581 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
     2641 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
     2701 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
     2761 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
     2821 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
     2881 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
     2941 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
     3001 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
     3061 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
     3121 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
     3181 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
     3241 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
     3301 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
     3361 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaagttacca cctttaatta
     3421 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
     3481 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
     3541 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
     3601 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
     3661 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     3721 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     3781 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     3841 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
     3901 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     3961 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
     4021 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
     4081 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     4141 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     4201 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     4261 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     4321 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     4381 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     4441 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
     4501 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
     4561 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
     4621 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
     4681 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
     4741 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
     4801 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
     4861 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
     4921 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
     4981 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
     5041 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
     5101 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
     5161 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
     5221 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
     5281 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
     5341 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
     5401 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
     5461 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
     5521 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
     5581 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
     5641 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
     5701 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
     5761 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
     5821 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
     5881 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
     5941 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
     6001 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     6061 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     6121 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     6181 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     6241 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     6301 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     6361 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     6421 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     6481 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
     6541 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     6601 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     6661 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     6721 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     6781 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     6841 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     6901 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
     6961 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     7021 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     7081 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     7141 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     7201 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     7261 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     7321 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     7381 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     7441 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     7501 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     7561 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     7621 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     7681 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     7741 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     7801 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     7861 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     7921 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     7981 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     8041 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     8101 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     8161 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     8221 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     8281 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     8341 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     8401 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     8461 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     8521 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     8581 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     8641 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     8701 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     8761 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     8821 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     8881 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     8941 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
     9001 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     9061 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     9121 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
     9181 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
     9241 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
     9301 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
     9361 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
     9421 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
     9481 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
     9541 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
     9601 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
     9661 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
     9721 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
     9781 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
     9841 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
     9901 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
     9961 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
    10021 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
    10081 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
    10141 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
    10201 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
    10261 aagtctgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
    10321 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
    10381 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
    10441 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
    10501 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
    10561 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
    10621 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
    10681 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
    10741 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
    10801 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
    10861 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
    10921 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
    10981 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
    11041 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    11101 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    11161 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    11221 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    11281 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    11341 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    11401 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    11461 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    11521 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    11581 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    11641 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    11701 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    11761 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    11821 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
    11881 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
    11941 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    12001 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    12061 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    12121 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    12181 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    12241 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    12301 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    12361 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    12421 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    12481 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    12541 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    12601 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    12661 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    12721 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    12781 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    12841 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    12901 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    12961 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
    13021 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
    13081 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
    13141 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
    13201 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
    13261 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
    13321 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
    13381 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
    13441 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
    13501 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
    13561 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
    13621 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
    13681 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
    13741 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
    13801 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
    13861 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
    13921 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
    13981 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
    14041 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
    14101 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
    14161 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
    14221 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
    14281 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
    14341 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
    14401 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
    14461 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
    14521 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
    14581 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
    14641 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
    14701 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
    14761 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
    14821 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
    14881 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
    14941 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
    15001 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
    15061 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
    15121 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
    15181 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
    15241 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
    15301 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
    15361 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
    15421 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
    15481 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
    15541 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
    15601 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
    15661 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
    15721 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
    15781 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
    15841 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
    15901 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
    15961 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
    16021 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
    16081 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
    16141 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
    16201 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
    16261 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
    16321 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
    16381 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
    16441 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
    16501 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
    16561 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
    16621 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
    16681 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
    16741 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
    16801 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
    16861 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
    16921 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
    16981 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
    17041 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
    17101 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
    17161 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
    17221 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
    17281 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
    17341 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
    17401 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
    17461 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
    17521 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
    17581 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
    17641 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
    17701 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
    17761 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
    17821 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
    17881 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
    17941 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
    18001 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
    18061 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
    18121 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
    18181 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
    18241 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
    18301 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
    18361 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
    18421 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
    18481 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
    18541 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
    18601 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
    18661 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
    18721 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
    18781 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
    18841 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
    18901 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
    18961 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
    19021 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
    19081 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
    19141 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
    19201 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
    19261 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
    19321 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
    19381 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
    19441 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
    19501 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
    19561 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
    19621 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
    19681 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
    19741 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
    19801 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
    19861 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
    19921 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
    19981 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
    20041 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
    20101 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
    20161 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
    20221 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
    20281 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
    20341 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
    20401 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
    20461 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
    20521 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
    20581 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
    20641 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
    20701 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
    20761 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
    20821 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
    20881 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
    20941 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
    21001 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
    21061 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
    21121 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
    21181 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
    21241 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
    21301 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
    21361 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
    21421 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
    21481 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
    21541 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
    21601 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
    21661 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
    21721 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
    21781 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
    21841 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
    21901 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
    21961 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
    22021 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
    22081 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
    22141 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
    22201 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
    22261 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
    22321 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
    22381 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
    22441 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
    22501 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
    22561 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
    22621 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
    22681 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
    22741 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
    22801 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
    22861 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
    22921 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
    22981 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
    23041 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    23101 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    23161 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    23221 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    23281 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    23341 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    23401 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    23461 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    23521 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    23581 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    23641 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    23701 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    23761 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    23821 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    23881 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    23941 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    24001 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    24061 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    24121 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    24181 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    24241 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    24301 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    24361 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    24421 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    24481 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    24541 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    24601 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    24661 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    24721 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    24781 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    24841 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    24901 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    24961 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    25021 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    25081 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    25141 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    25201 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    25261 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    25321 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    25381 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    25441 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    25501 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    25561 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    25621 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    25681 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    25741 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    25801 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    25861 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    25921 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    25981 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    26041 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    26101 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    26161 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    26221 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    26281 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    26341 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    26401 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    26461 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    26521 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    26581 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    26641 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    26701 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    26761 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    26821 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    26881 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    26941 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    27001 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    27061 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    27121 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    27181 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    27241 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    27301 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    27361 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    27421 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    27481 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    27541 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    27601 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    27661 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    27721 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    27781 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    27841 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    27901 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    27961 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    28021 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
    28081 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    28141 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    28201 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    28261 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    28321 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    28381 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
    28441 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
    28501 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    28561 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    28621 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    28681 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    28741 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    28801 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    28861 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    28921 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    28981 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    29041 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    29101 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    29161 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    29221 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    29281 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    29341 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    29401 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    29461 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    29521 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    29581 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    29641 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    29701 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    29761 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    29821 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    29881 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    29941 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    30001 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    30061 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    30121 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    30181 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    30241 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    30301 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    30361 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    30421 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    30481 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    30541 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    30601 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    30661 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    30721 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    30781 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    30841 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    30901 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    30961 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    31021 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    31081 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    31141 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    31201 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    31261 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    31321 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    31381 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    31441 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    31501 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    31561 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    31621 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    31681 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    31741 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    31801 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    31861 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    31921 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    31981 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    32041 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    32101 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    32161 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    32221 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    32281 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    32341 atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    32401 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    32461 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    32521 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    32581 cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga
    32641 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    32701 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    32761 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    32821 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa
    32881 tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt
    32941 tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt
    33001 aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt
    33061 aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt
    33121 cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga
    33181 attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt
    33241 ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat
    33301 tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga
    33361 agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt
    33421 ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag
    33481 gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa
    33541 aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc
    33601 gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg
    33661 gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa
    33721 cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc
    33781 ttattacctg tagtagattc taataaggaa caagaccaag aattactaga agctatttac
    33841 caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct
    33901 ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat
    33961 gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta
    34021 ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca
    34081 gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat
    34141 tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct
    34201 ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca
    34261 gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag
    34321 attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg
    34381 cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt
    34441 tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa
    34501 gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg
    34561 ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa
    34621 cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct
    34681 gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt
    34741 cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa
    34801 caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa
    34861 atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa
    34921 gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta
    34981 cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga gttactggat
    35041 aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc
    35101 aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct
    35161 tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc
    35221 gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca
    35281 ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct
    35341 tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca
    35401 aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa
    35461 tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat
    35521 tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa
    35581 ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac
    35641 ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa
    35701 ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg
    35761 agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc
    35821 aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag
    35881 cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag
    35941 acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct
    36001 ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc
    36061 aagatataga agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac
    36121 atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc
    36181 aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg
    36241 tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt
    36301 gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag
    36361 acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt
    36421 tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat
    36481 attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag
    36541 aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag
    36601 caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata
    36661 tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg
    36721 tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga
    36781 acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc
    36841 gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag
    36901 gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga
    36961 tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta
    37021 agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac
    37081 ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca
    37141 ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc
    37201 tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt
    37261 cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag
    37321 atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa
    37381 aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag
    37441 ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc
    37501 tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac
    37561 tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc
    37621 acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata
    37681 tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact
    37741 tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt
    37801 gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact
    37861 aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac
    37921 aactctttta acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata
    37981 tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag
    38041 tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct
    38101 gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg
    38161 ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt
    38221 tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata
    38281 gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga
    38341 tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc
    38401 ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa
    38461 tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac
    38521 aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc
    38581 aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa
    38641 taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat
    38701 gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat
    38761 tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt
    38821 ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc
    38881 cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt
    38941 tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag
    39001 cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg
    39061 aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa
    39121 tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg
    39181 aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact
    39241 gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg
    39301 cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat
    39361 ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca
    39421 taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat
    39481 tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga
    39541 tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc
    39601 gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt
    39661 tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag
    39721 gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg
    39781 ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt
    39841 cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt
    39901 aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt
    39961 aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta
    40021 ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca
    40081 tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc
    40141 cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca
    40201 aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac
    40261 ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat
    40321 gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg
    40381 cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga
    40441 atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga
    40501 aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt
    40561 tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa
    40621 atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagga a
//
LOCUS       DAZAJZ010000014        37996 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000014 DAZAJZ010000000
VERSION     DAZAJZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.014"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG86D_000751"
     CDS             complement(159..1469)
                     /locus_tag="ACG86D_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN3139923.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACG86D_000752"
     CDS             complement(1481..2899)
                     /locus_tag="ACG86D_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3139924.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="ACG86D_000753"
     CDS             complement(3139..4707)
                     /locus_tag="ACG86D_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN3139925.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="ACG86D_000754"
     CDS             complement(4711..5121)
                     /locus_tag="ACG86D_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN3139926.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="ACG86D_000755"
     CDS             complement(5118..6041)
                     /locus_tag="ACG86D_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN3139927.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="ACG86D_000756"
     CDS             complement(6038..6916)
                     /locus_tag="ACG86D_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN3139928.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="ACG86D_000757"
     CDS             complement(6903..8240)
                     /locus_tag="ACG86D_000757"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN3139929.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="ACG86D_000758"
     CDS             complement(8243..9346)
                     /locus_tag="ACG86D_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3139930.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="ACG86D_000759"
     CDS             complement(9420..10928)
                     /locus_tag="ACG86D_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3139931.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="ACG86D_000760"
     CDS             complement(10894..11586)
                     /locus_tag="ACG86D_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN3139932.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="ACG86D_000761"
     CDS             complement(11608..12582)
                     /locus_tag="ACG86D_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139933.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="ACG86D_000762"
     CDS             12882..13754
                     /locus_tag="ACG86D_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3139934.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="ACG86D_000763"
     CDS             complement(14212..16410)
                     /locus_tag="ACG86D_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3139935.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="ACG86D_000764"
     CDS             16642..17634
                     /locus_tag="ACG86D_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3139936.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="ACG86D_000765"
     CDS             17694..19115
                     /locus_tag="ACG86D_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN3139937.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="ACG86D_000766"
     CDS             19108..20607
                     /locus_tag="ACG86D_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN3139938.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="ACG86D_000767"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="ACG86D_000767"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN3139939.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="ACG86D_000768"
     CDS             22199..22882
                     /locus_tag="ACG86D_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN3139940.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="ACG86D_000769"
     CDS             22884..23720
                     /locus_tag="ACG86D_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3139941.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="ACG86D_000770"
     CDS             23731..25485
                     /locus_tag="ACG86D_000770"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139942.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="ACG86D_000771"
     CDS             complement(25761..26102)
                     /locus_tag="ACG86D_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3139943.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="ACG86D_000772"
     CDS             complement(26170..28341)
                     /locus_tag="ACG86D_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN3139944.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="ACG86D_000773"
     CDS             28552..29409
                     /locus_tag="ACG86D_000773"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3139945.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="ACG86D_000774"
     CDS             complement(29480..30337)
                     /locus_tag="ACG86D_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3139946.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="ACG86D_000775"
     CDS             complement(30322..33024)
                     /locus_tag="ACG86D_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN3139947.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="ACG86D_000776"
     CDS             complement(33037..34326)
                     /locus_tag="ACG86D_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN3139948.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="ACG86D_000777"
     CDS             34476..35522
                     /locus_tag="ACG86D_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3139949.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="ACG86D_000778"
     CDS             35524..37677
                     /locus_tag="ACG86D_000778"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN3139950.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11227 a         7816 c         6765 g        12188 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatttgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagata tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcttcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcagggcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agcatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 aaccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgac tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca gagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccattta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca tttttg
//
LOCUS       DAZAJZ010000015        37225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000015 DAZAJZ010000000
VERSION     DAZAJZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.015"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACG86D_000779"
     CDS             19..540
                     /locus_tag="ACG86D_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3139951.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACG86D_000780"
     CDS             637..858
                     /locus_tag="ACG86D_000780"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3139952.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACG86D_000781"
     CDS             complement(1015..1179)
                     /locus_tag="ACG86D_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFN3139953.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACG86D_000782"
     CDS             1282..1803
                     /locus_tag="ACG86D_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN3139954.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACG86D_000783"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACG86D_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACG86D_000784"
     CDS             complement(2270..2428)
                     /locus_tag="ACG86D_000784"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFN3139955.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACG86D_000785"
     CDS             2521..3282
                     /locus_tag="ACG86D_000785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3139956.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACG86D_000786"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACG86D_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACG86D_000787"
     CDS             4837..6057
                     /locus_tag="ACG86D_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3139957.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACG86D_000788"
     CDS             6092..6769
                     /locus_tag="ACG86D_000788"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN3139958.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACG86D_000789"
     CDS             6766..7962
                     /locus_tag="ACG86D_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3139959.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACG86D_000790"
     CDS             7977..8300
                     /locus_tag="ACG86D_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3139960.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACG86D_000791"
     CDS             8304..8639
                     /locus_tag="ACG86D_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3139961.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACG86D_000792"
     CDS             8636..9031
                     /locus_tag="ACG86D_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN3139962.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACG86D_000793"
     CDS             9028..9399
                     /locus_tag="ACG86D_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139963.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACG86D_000794"
     CDS             9415..9975
                     /locus_tag="ACG86D_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN3139964.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACG86D_000795"
     CDS             10102..10413
                     /locus_tag="ACG86D_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139965.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACG86D_000796"
     CDS             10437..10628
                     /locus_tag="ACG86D_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139966.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACG86D_000797"
     CDS             10628..14473
                     /locus_tag="ACG86D_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFN3139967.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACG86D_000798"
     CDS             14474..15346
                     /locus_tag="ACG86D_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFN3139968.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACG86D_000799"
     CDS             15343..16392
                     /locus_tag="ACG86D_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139969.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACG86D_000800"
     CDS             16396..17220
                     /locus_tag="ACG86D_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139970.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACG86D_000801"
     CDS             17238..19370
                     /locus_tag="ACG86D_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN3139971.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACG86D_000802"
     CDS             19387..19536
                     /locus_tag="ACG86D_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139972.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACG86D_000803"
     CDS             19533..19979
                     /locus_tag="ACG86D_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139973.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACG86D_000804"
     CDS             19981..20118
                     /locus_tag="ACG86D_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN3139974.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACG86D_000805"
     CDS             20155..20448
                     /locus_tag="ACG86D_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3139975.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACG86D_000806"
     CDS             20471..20668
                     /locus_tag="ACG86D_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN3139976.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACG86D_000807"
     CDS             20679..21698
                     /locus_tag="ACG86D_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3139977.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="ACG86D_000808"
     CDS             21955..22602
                     /locus_tag="ACG86D_000808"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3139978.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="ACG86D_000809"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="ACG86D_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            23312..24004
                     /locus_tag="ACG86D_000810"
     CDS             23312..24004
                     /locus_tag="ACG86D_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3139979.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(24286..24358)
                     /locus_tag="ACG86D_000811"
     tRNA            complement(24286..24358)
                     /locus_tag="ACG86D_000811"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24323..24325),aa:Lys,seq:ctt)
     gene            24739..25308
                     /locus_tag="ACG86D_000812"
     CDS             24739..25308
                     /locus_tag="ACG86D_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN3139980.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            25295..26155
                     /locus_tag="ACG86D_000813"
     CDS             25295..26155
                     /locus_tag="ACG86D_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3139981.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            26345..27049
                     /gene="yycF"
                     /locus_tag="ACG86D_000814"
     CDS             26345..27049
                     /gene="yycF"
                     /locus_tag="ACG86D_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN3139982.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            27054..28889
                     /gene="walK"
                     /locus_tag="ACG86D_000815"
     CDS             27054..28889
                     /gene="walK"
                     /locus_tag="ACG86D_000815"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN3139983.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            28886..30202
                     /gene="yycH"
                     /locus_tag="ACG86D_000816"
     CDS             28886..30202
                     /gene="yycH"
                     /locus_tag="ACG86D_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN3139984.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            30203..31072
                     /locus_tag="ACG86D_000817"
     CDS             30203..31072
                     /locus_tag="ACG86D_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN3139985.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            31127..31936
                     /locus_tag="ACG86D_000818"
     CDS             31127..31936
                     /locus_tag="ACG86D_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3139986.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(32039..32668)
                     /locus_tag="ACG86D_000819"
     CDS             complement(32039..32668)
                     /locus_tag="ACG86D_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3139987.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            32838..34130
                     /locus_tag="ACG86D_000820"
     CDS             32838..34130
                     /locus_tag="ACG86D_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3139988.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(34206..34868)
                     /locus_tag="ACG86D_000821"
     CDS             complement(34206..34868)
                     /locus_tag="ACG86D_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN3139989.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            35093..35377
                     /gene="groES"
                     /locus_tag="ACG86D_000822"
     CDS             35093..35377
                     /gene="groES"
                     /locus_tag="ACG86D_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN3139990.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            35428..37053
                     /gene="groL"
                     /locus_tag="ACG86D_000823"
     CDS             35428..37053
                     /gene="groL"
                     /locus_tag="ACG86D_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN3139991.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        12853 a         6293 c         8079 g        10000 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa aatattttat
    23161 actctcaaat gtttgtatgc agttacatct gactttattc gaattaagga aaagatttat
    23221 aaaaataaaa taaaaaaata tatatttaaa ttgacttatt gtgaaatcat taactataat
    23281 tagttcagtt tatgttaagg agaatgatga tatgaataag aaaattgtag taagtggatt
    23341 aattataggt ggattagtgt tagcagggat cgcagggaaa aactttgccg atgacatttt
    23401 gttatggggt ggagaagata atattgaaca aatcaatcat aatttggagt cattagataa
    23461 agcactagaa aataaagagc aaaatattag tagtttaaat tcacgattat cttcaaataa
    23521 tcaagaactt gagcaagcaa aggctaacgt tgaagaatat aaacaaaaag tgttgacttt
    23581 ggaaaatgat aaaaatcaat taactataga gaaaacaaat ttagagaatc aattatcagg
    23641 aaaaaatgct gaattgcaag gtaaacaaaa tgaaatcaat gctaaaattg atgaaataaa
    23701 tcaaaaaata gctgaaataa atcaattaaa cactgattgg aataatcgtt tacaaacaga
    23761 acaaaataaa tttaatgatg ctcaaaatac tgttaatagt ttaaatcaac aaattaatca
    23821 attgtcaaat gaaaaaaata ctattaaaaa ccaattgcaa agtactgaac aaaaactaga
    23881 aaaagcgcaa tcagaaaaca gtaatttgaa taattatatt gaaaaactgg aaaaagccaa
    23941 aagcgatgta gaagacacag caaacagatc acaagaaatc gtgactgaac atacaggaaa
    24001 atagatgcat tttttagttt aaatttcttt aacaaaatag tattttgttg ctaaataatt
    24061 tttattttaa tattctacaa tgatatctcc cctcaaaatg gaggggaagt acatactgtg
    24121 ataatctaca ggtttattga ctgattagga tgttattaaa aaataaaata ctaaaatgca
    24181 tacccgaatt tcatacccga aaaataaaaa atgccaccac gtacactctt ttcgaaacta
    24241 cctaagtagc caagaatgta ctggtgacgt gggtttggca cccaatgggc cgtgaggggc
    24301 tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt
    24361 acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag
    24421 aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct
    24481 cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt
    24541 ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa
    24601 aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca
    24661 cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg
    24721 aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa
    24781 ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac
    24841 caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca
    24901 ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt
    24961 aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa
    25021 gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt
    25081 cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa
    25141 ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt
    25201 gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt
    25261 ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt
    25321 agtagattca ggtacggatg tgccagaaga attgataaaa aaatacgaga tgtatatgat
    25381 cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca ttacctctga
    25441 agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc
    25501 gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac
    25561 aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga
    25621 agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc
    25681 gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct
    25741 gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca
    25801 aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc
    25861 aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa
    25921 aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt
    25981 caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact
    26041 taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt
    26101 agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa
    26161 aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa
    26221 aataataaag tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg
    26281 aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct
    26341 ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt
    26401 caatttagcc aaagaaggct atgatgttta tactgcatat gatggggaag aagccttgga
    26461 aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga
    26521 cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac
    26581 agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt
    26641 gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg
    26701 tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt
    26761 gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca
    26821 tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga
    26881 acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt
    26941 aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt
    27001 gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa
    27061 gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt
    27121 tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga
    27181 tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc
    27241 cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat
    27301 ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga
    27361 tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg
    27421 ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg
    27481 tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt
    27541 acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct
    27601 ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt
    27661 cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact
    27721 acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca
    27781 atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt
    27841 tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag
    27901 tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg
    27961 gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag
    28021 aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga
    28081 tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag
    28141 ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct
    28201 ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat
    28261 tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag
    28321 atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata
    28381 caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc
    28441 ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat
    28501 ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata
    28561 atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat
    28621 tagaaacgca taataacgtg gttttaagta tcaccgatca aggattgggg attcctaaaa
    28681 aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac
    28741 aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg
    28801 ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg
    28861 aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact
    28921 cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg
    28981 aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg
    29041 aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca
    29101 agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt
    29161 tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct
    29221 gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc
    29281 atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac
    29341 tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct
    29401 cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa
    29461 agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac
    29521 gaaacattcg atcaaactga aaaagtatag ttatatcctg tcacagcaac cctatacact
    29581 ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga
    29641 attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga
    29701 ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc
    29761 ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa
    29821 tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg
    29881 acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac
    29941 cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac
    30001 gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt
    30061 gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc
    30121 tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt
    30181 acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc
    30241 ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa
    30301 caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat
    30361 cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca
    30421 gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc
    30481 aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga
    30541 aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa
    30601 agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga
    30661 agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac
    30721 agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat
    30781 gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga
    30841 ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg
    30901 gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt
    30961 ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagtg tcaacgcttt
    31021 cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga
    31081 gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa
    31141 accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga
    31201 aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt
    31261 gatggctcaa attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca
    31321 tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc
    31381 caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa
    31441 acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg
    31501 cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt
    31561 cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc
    31621 cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc
    31681 gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc
    31741 acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag
    31801 caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa
    31861 agattttggc gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga
    31921 gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa
    31981 tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt
    32041 atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta
    32101 atgcaatcgc gtaatctgcc atgctgatcg tactgtcgcc agcagcgttt ctttgtaaat
    32161 aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg
    32221 gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca
    32281 tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg
    32341 tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg
    32401 ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt
    32461 tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact
    32521 caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga
    32581 ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc
    32641 cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt
    32701 aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct
    32761 ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa
    32821 gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat
    32881 ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa
    32941 taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc
    33001 agtggataga caaaacagga ttttctatgt attttacaca agtataccag gctacatttg
    33061 atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca
    33121 tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac
    33181 taaatactca gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa
    33241 ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc
    33301 aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg
    33361 ttatagtcac ttttacgctt atcggttcag cggtttttca acagttgttc agaatgactg
    33421 ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt
    33481 ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat
    33541 ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta gcgaatgcag
    33601 aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc
    33661 aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt
    33721 ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac
    33781 cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga
    33841 tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt
    33901 tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga
    33961 tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt
    34021 caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa
    34081 aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa
    34141 tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt
    34201 catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt
    34261 cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag
    34321 aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc
    34381 aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata
    34441 ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa
    34501 gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg
    34561 ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt
    34621 ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc
    34681 gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac
    34741 atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg
    34801 atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt
    34861 aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat
    34921 tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg
    34981 atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc
    35041 gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa
    35101 accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg
    35161 aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg
    35221 tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca
    35281 agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat
    35341 cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc
    35401 aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca
    35461 cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga
    35521 cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat
    35581 ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa
    35641 cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca
    35701 actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat
    35761 cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat
    35821 aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg
    35881 ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt
    35941 gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg
    36001 cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct
    36061 gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt
    36121 ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat
    36181 gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta
    36241 gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct
    36301 attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa agatgtaaca
    36361 atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta
    36421 gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt
    36481 gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca
    36541 ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa
    36601 ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca
    36661 ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga
    36721 gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc
    36781 gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta
    36841 ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta
    36901 gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta
    36961 ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca
    37021 atggatccat caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact
    37081 gatcattagt gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat
    37141 aagaagaaat tcacggaaat tgcttttcaa atttttgtga atttcagctt atttccgaag
    37201 gagttgcttc tgcttccgct gttta
//
LOCUS       DAZAJZ010000016        35349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000016 DAZAJZ010000000
VERSION     DAZAJZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.016"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..1807
                     /locus_tag="ACG86D_000824"
     CDS             146..1807
                     /locus_tag="ACG86D_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3139992.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1877..2239)
                     /locus_tag="ACG86D_000825"
     CDS             complement(1877..2239)
                     /locus_tag="ACG86D_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN3139993.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2307..3248
                     /gene="opp3b"
                     /locus_tag="ACG86D_000826"
     CDS             2307..3248
                     /gene="opp3b"
                     /locus_tag="ACG86D_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN3139994.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3249..4292
                     /locus_tag="ACG86D_000827"
     CDS             3249..4292
                     /locus_tag="ACG86D_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3139995.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4298..5368
                     /locus_tag="ACG86D_000828"
     CDS             4298..5368
                     /locus_tag="ACG86D_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139996.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5358..6311
                     /locus_tag="ACG86D_000829"
     CDS             5358..6311
                     /locus_tag="ACG86D_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3139997.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6323..7333
                     /locus_tag="ACG86D_000830"
     CDS             6323..7333
                     /locus_tag="ACG86D_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN3139998.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7618..8439)
                     /locus_tag="ACG86D_000831"
     CDS             complement(7618..8439)
                     /locus_tag="ACG86D_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN3139999.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8536..8814)
                     /locus_tag="ACG86D_000832"
     CDS             complement(8536..8814)
                     /locus_tag="ACG86D_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140000.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9082..11091
                     /gene="metG"
                     /locus_tag="ACG86D_000833"
     CDS             9082..11091
                     /gene="metG"
                     /locus_tag="ACG86D_000833"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN3140001.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11570..12157)
                     /locus_tag="ACG86D_000834"
     CDS             complement(11570..12157)
                     /locus_tag="ACG86D_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140002.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12332..13654
                     /locus_tag="ACG86D_000835"
     CDS             12332..13654
                     /locus_tag="ACG86D_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3140003.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13809..14585
                     /locus_tag="ACG86D_000836"
     CDS             13809..14585
                     /locus_tag="ACG86D_000836"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN3140004.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14578..15147
                     /gene="rnmV"
                     /locus_tag="ACG86D_000837"
     CDS             14578..15147
                     /gene="rnmV"
                     /locus_tag="ACG86D_000837"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN3140005.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15140..16024
                     /gene="rsmA"
                     /locus_tag="ACG86D_000838"
     CDS             15140..16024
                     /gene="rsmA"
                     /locus_tag="ACG86D_000838"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN3140006.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16475..17104
                     /locus_tag="ACG86D_000839"
     CDS             16475..17104
                     /locus_tag="ACG86D_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140007.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17228..18337)
                     /locus_tag="ACG86D_000840"
     CDS             complement(17228..18337)
                     /locus_tag="ACG86D_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140008.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18546..18968
                     /gene="mgsA"
                     /locus_tag="ACG86D_000841"
     CDS             18546..18968
                     /gene="mgsA"
                     /locus_tag="ACG86D_000841"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN3140009.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19155..19253
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19291..19827
                     /locus_tag="ACG86D_000842"
     CDS             19291..19827
                     /locus_tag="ACG86D_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN3140010.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19867..21630)
                     /locus_tag="ACG86D_000843"
     CDS             complement(19867..21630)
                     /locus_tag="ACG86D_000843"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140011.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21633..23348)
                     /locus_tag="ACG86D_000844"
     CDS             complement(21633..23348)
                     /locus_tag="ACG86D_000844"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140012.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24127..24351)
                     /locus_tag="ACG86D_000845"
     CDS             complement(24127..24351)
                     /locus_tag="ACG86D_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140013.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24542..27319
                     /locus_tag="ACG86D_000846"
     CDS             24542..27319
                     /locus_tag="ACG86D_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3140014.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27400..27819
                     /locus_tag="ACG86D_000847"
     CDS             27400..27819
                     /locus_tag="ACG86D_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3140015.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27831..28301
                     /locus_tag="ACG86D_000848"
     CDS             27831..28301
                     /locus_tag="ACG86D_000848"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3140016.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28318..29097
                     /locus_tag="ACG86D_000849"
     CDS             28318..29097
                     /locus_tag="ACG86D_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3140017.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29084..29905
                     /locus_tag="ACG86D_000850"
     CDS             29084..29905
                     /locus_tag="ACG86D_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3140018.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29922..30971
                     /locus_tag="ACG86D_000851"
     CDS             29922..30971
                     /locus_tag="ACG86D_000851"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3140019.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30984..31988
                     /locus_tag="ACG86D_000852"
     CDS             30984..31988
                     /locus_tag="ACG86D_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3140020.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32621..33640
                     /locus_tag="ACG86D_000853"
     CDS             32621..33640
                     /locus_tag="ACG86D_000853"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN3140021.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33683..34564
                     /gene="galU"
                     /locus_tag="ACG86D_000854"
     CDS             33683..34564
                     /gene="galU"
                     /locus_tag="ACG86D_000854"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN3140022.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            34864..35241
                     /locus_tag="ACG86D_000855"
     CDS             34864..35241
                     /locus_tag="ACG86D_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140023.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT        11589 a         6220 c         7258 g        10282 t
ORIGIN      
        1 aattccttga aacctcacga aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg
       61 attttttaat aattattcat taaactctta aacaacttca gaaaatatta attattctga
      121 aaattccaat aaaggggtac gtaaaatgaa aaagaaattt acatttggtt tggttgcggt
      181 atgcggcctt gtacttgctg gatgttatgg cggcagttca gccacaagca gtagttcagc
      241 aaaaagcggt tcagcagatg gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc
      301 tacagctgac ttatcagttg caacagatac aatcagtttt acagcattga ataacgttta
      361 tgaaggaatt taccgtcttg atgaagacag caaaccacag ccggcaggag ctagcgagtt
      421 ggcagaagtc agtgaagacg gattaactta caaaatcaaa ttacgagaag acgcaaaatg
      481 gtcaaatggt gatcctgtta cagcagcgga ttacgtttat ggctggcaac gaacagtaga
      541 cccagctaca gcttctgaat atgcttacct gtatgcgcca gttgaaaatg cagaagacat
      601 tacagctgga aagaaagata aatcagaact agggatcaaa gcagttggtg actatgaatt
      661 agaaatcaaa ttgacgaaac aaacaccata tttccaatat ctgcttgctt tcccatcatt
      721 tttcccacaa aaacaatccg tagtggaaga aaatggggat gcctatgcat ctgcaagtga
      781 taaagctgtt tataatggac cattcacatt agaagatttt gatggaccag gaacagatac
      841 agaatggact tataagaaaa atgatcatta ctgggacaaa gatacagtaa aactttctga
      901 aatcaaagtc agtgtagtaa aagaatcttc tactgcattg aacttattca aagatggcca
      961 agcagatgac gtgatcttgt ctggtgagtt agcacagcaa aacgcaaacg atccagctta
     1021 tacttctgtt aaagaagcgc ggacaagcta tatcgaattc aatcaacgtg aaaagaattc
     1081 accattcaac aatgtgaacc taagaaaagc tatttcttat tctatcgatc gtgaagcttt
     1141 agtaaaacaa gtactaggtg acggatcagt tgtctctaca ggattgatcc catcagatat
     1201 ggtaaaaagt ccaacaacga acgaagactt tgcaaaagaa gctggaaaat tagtttctta
     1261 tgacaaagat aaagcaaaag aatattggga aaaagcgaaa aaagaattgg gcgttgattc
     1321 tttagaattt gatttgatgg cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt
     1381 tcaaaactca atccaagaaa acttggatgg cgtaacagtc aaaccaacac ctgttccatt
     1441 ctctgtacgt cttgaccgct caacttcggg tgattttgac gcagtactag gcggatgggc
     1501 tgctgactat gcagacccaa gcagctttac tgatttgttt gtcacaggta attcatacaa
     1561 ccgtggtcgc tggagcaatg cagaatatga cgaagcagtt aaagcagctt catcaacaga
     1621 cgctggcgat gaacaagccc gttgggatga tttccaaaaa gcaatcaaga tcatctctga
     1681 tgatatgggt gtcgttccgg tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat
     1741 caaaggtatc gttcaccatg cggcaggcgc ttcatgggat tacaaatgga catacgttga
     1801 agaataatcg ttaataccgt gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc
     1861 tcgttctcat attcacttat cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa
     1921 ttagtattcc ggcaatgatc agccaaaagc tgtacatacc gtagctggca gacgagagcg
     1981 tggaaaattc tggttttttc tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa
     2041 aatgatcgaa gaaacccgaa gaaaagaccc ataaaaaacc gccgataatc agaaacggca
     2101 atgaccataa gaatagatca ttagataatt gaatgagtgt aaaggaatgg ttgatgagat
     2161 tttttattaa aataaccaga atcagtagaa aagctactgc gtaggggata gatttttttt
     2221 catttgattg ctcctttatt tttggttcat tatctaataa tgtagcgaaa catctgtcag
     2281 aagtcaaaag ctagggggaa acattagtga atagttatat caaatatgta ttaaaacgtg
     2341 tctttttcat gatcatcacg ctttggttaa ttgcaacaat cacctttttc ttgatgcaat
     2401 tgctgccggg cacgccatat acgaatcagg aacgtttgag tcctgaaaca atcgagatgc
     2461 taaataaaca agtcggtttg gataaaccag ttatcgtaca atatgggatt tatctttcta
     2521 atttgcttca aggagatttt ggaatttcct tccaatttaa aaaccagcca gtcgcacatt
     2581 tgttagctgg aagaatcgga ccatccttgc agttagggtt acaggcaatt atctttggaa
     2641 cagtcctagg gacgatttta ggaacgatct ctgccatgaa gcaaaataca tgggcagata
     2701 catcttctac actcgtagcg atattaggtc gttccattcc aaactttgta tttgctgttt
     2761 tattacaata tatctttgca atcaaattac aagttttgcc aatcgcaaaa tgggatggct
     2821 ttgtgtacac aatcttacca acaattgcgt tggccatgtc gccgcttgct gactctgctc
     2881 ggtttatccg tacggaaatg gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag
     2941 caaaaggatt gagccgttgg gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc
     3001 cattgatgac tttattagga ccgttagctg tagcattgat gaccggatca ctggtggtag
     3061 aaaatatctt tgctattcct ggtatcgggg aacaattcgt taagtcaatc acaacaaatg
     3121 attatcctac gatcatggct gtaacgatcc tttactcatt tatgctgatt tttgttattt
     3181 tgatcgtgga tctattgtac ggtctagttg atccaagaat tcgtgtttca gaagggagcc
     3241 gaggataaat ggaaatgata ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg
     3301 aatttcttcc attacaaaaa aataccgaag aagaacgtga acagattact gcaccttctt
     3361 tgaattttat ccaagattca tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa
     3421 tggttttact attggtcatc attttgatct ctgtgatcac aatctttgtt tctcctcatg
     3481 atccaactgc ccaaaacgtg gcttatatca atttgcctcc tcgtattccg ggaatcaata
     3541 tcgatggatt gaatggaaaa gcaatggttg gtggtgaact tgttgataaa tatgctcaag
     3601 caaacgtccc ttcaaacgta aatttctttt taggaacaga cggtttggga cgtgatgtat
     3661 taagccgctt atttatggga atgagaatct cgttgctgat cgcatttatt gctgcattac
     3721 tagatgtaac gattggtgta gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg
     3781 ataacgtgat gcaacgtttt ttggaaatct tatcaggaat tccaaacctc gttgtcatga
     3841 ttttgatgtt ggtcgttttt gaaccaggaa ttttttcaat cgtggctgct atggccatca
     3901 caaactggat accaatggca cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag
     3961 aatatgtttt agctggattg acattaggtg aatcaagaat gaaaattgca ttcaaacata
     4021 ttttgccgaa tatctctagt gtcattatta ttcaaatgat gtttagtatt ccatctgcta
     4081 ttttcttcga agccttttta agctttatcg gtttaggtct tacgcctccg tcagcttcat
     4141 taggaacgat gttgagcgaa gggtacaaga cattcctgta tctgccttat ctattatgga
     4201 ttccggcagt aactttatcg gttatcatga ttggctttaa cttacttgct gatggattgc
     4261 gtgatgcctt tgatcctaaa atgaaagagt gaagacgatg acgaaaattt tagaagtgaa
     4321 agacttagaa atctcttttg atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa
     4381 ttttcatttg aataaaggag aaacattggc gatcgttggg gaatctggaa gtggaaaatc
     4441 agtgacgacc agaagcatca tgcgcttgct gtcaagcaac gccaatatag atgctggtca
     4501 aatcctcttt aaaggtaacg atatcgttgg aaaatcagaa agagagatgc aaaaaatccg
     4561 tggtaaagaa atagcgatga ttttccaaga tccaatgacc tctttagatc cgacgatgcc
     4621 cattgggaag caagtcgctg agtcccttag aaagcataat aaagtttcaa aaaaagaggg
     4681 acaaaaagct gcattagaat tgcttaaatt agttggaata cccgaagctg agaaacgtat
     4741 caacagttat ccgcatcagt tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc
     4801 actggtatgt tatccggaaa tcctgattgc tgatgaacct acaacagcgc ttgatgtgac
     4861 tattcaagca cagattttgg agctgctgaa agaaattcag acaaaaatcg atacatcaat
     4921 tatctttatc actcatgacc ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat
     4981 gtatggagga agaatcgttg aagttggaac atcggaagaa attttctaca acccgcaaca
     5041 tccctatact tggggactgc ttggttcaat gccgacatta gatagtgcga acgatcgatt
     5101 gtatgctatt cctggttctc caccggattt attgaacccg ccaaaaggag acgcattcta
     5161 tccaagaaat gaattcgcga tgaaaatcga tgcagaacaa gaaccaccat tttttgaact
     5221 atcaaaaaca cacaaggcag caacttggct acttgctccg caggcaccca aagtgacacc
     5281 gccagaagaa atccaacgtc gctgggctat ctttaaagag aaacaaaaac atacttacgg
     5341 tcaaggagga attgctgatg ccaaataaac tacttgaagt taaaggattg aaacagtatt
     5401 tcaatgtagg aaaaaagaat gaagtacatg ctgtaaatga catcagcttt cacatttatg
     5461 aaggagaaac attcggttta gttggagaat ctggaagcgg aaaatccact actggccgta
     5521 cgatcattcg cttgaatgaa cctactgatg gtgagatatt attcgacggt caagatataa
     5581 cgaagatcaa agataaaaaa ggtttgacga aattccggca tgatgttcag atgatctttc
     5641 aagatccata cgcttcgttg aatcctcgta tgaaagtcag agatatcatt gccgaaggaa
     5701 ttgatgttaa tggactagct aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat
     5761 tgagaactgt tggattgaat ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg
     5821 ggcaacgcca gcgtattggt attgcacgag cattggcagt aaaaccgcgt tttatcattt
     5881 gtgatgaacc aatttctgca ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc
     5941 aagatctcca aaaagaacat caattgacct atctatttat tgcacatgat ttgtcgatgg
     6001 ttaaacatat tagtgaccga atcggtgtaa tgcataacgg cttgttatta gagatgggaa
     6061 caagtgatga aatctataat cacggtgttc atccttatac agaaagtttg ttatctgcta
     6121 ttcctctacc agatccagac catgagagaa aacgcagaag aatcaaatat caacctgaac
     6181 cagatgacgg acaagtacgt caacttcgtg aaatcgcacc agaacatttt gtctatgcaa
     6241 ctgagcagga agtcccatat tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg
     6301 ttgctaacta ggagggacat agatgctaaa agcttttaaa ttccgtatgt atccgacaga
     6361 agaacaaaaa cagcaattga ttcgtacttt tggatgtgct cgatttacct ataaccattt
     6421 gctgaaaaag cggcagaaaa gttggcaaca aacaggagta gccaattttt cgctcacacc
     6481 cgctacatta aaaaaagaat acccattctt aaaagaagta gacagtcttg ctttagcaaa
     6541 tgcacagctt aatttggatc gggcttttcg caattatttc aagggacgtg ccagcttccc
     6601 aaagcttaaa acgaaaaaaa gtatgtggca atcttatacg accaacaacc aaacccgtac
     6661 gatctatctg gaaaatggct acttaaagtt accaaaacaa aaagaactaa tcaagataaa
     6721 tcagcatcgg ccagttgaag gatcgattcg ctccgctacg atttctgctc gatataatga
     6781 agaattttat gtcgcactac tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa
     6841 atggatcggg atcgcctatc atccgaagac attgatccaa acatctcgac ctctagaagt
     6901 aactttacca aaatttcacc agacagaaga aaagctgcaa catgcacagc ggaaattgaa
     6961 tgtgaaagtc cgctccgctc accatagaaa gatcaggtta gatcaggcaa gcaattatca
     7021 gaaacaaaag agaaaagtaa tggatcttta tctgaaacaa aagaaccagc gagaagatta
     7081 tcttgaacaa ttatctggga aattggtcaa acaatatgac tacttatttg tagaatcttt
     7141 tccaaaagaa gaggcacatg ctgatttttc tattcacgac tggcataaat taataacaaa
     7201 attgcgttat aagtcccaat ggtataataa aaaatttctt ttgatcaata ccgacggagc
     7261 agaagaaagc aattccgtaa gaaagagcca agtagtagaa aaaatgggca gacattcggt
     7321 tatcaaggga taaaaaatga atgaaactga taggagcgtt agggttagcc ttgttaataa
     7381 aaagtcttaa tgacttttgt ttctaggaat ttttcttttg aagaaaagct cactaattat
     7441 gacaaaccaa taaaacaata gcagaaaaag ttcgtacgag ctttttctgc tattgttaat
     7501 tttttattca gaaagtttac gaatacatca aaattctgaa acaaaaaacg taactcacaa
     7561 agagttgttt ttgcgttttt tcttgaatga ttaggtctga atcttttgct cacaacctta
     7621 ttcatggtgc cccggcttga gctgatagat ttttgaagga cggccgattc ctacaggacg
     7681 ttcaccgatc tcatcgaaat attggagcat agctttctta aaattggaat gatcgatcgt
     7741 tttataatcg atccctaaga atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc
     7801 tttacctaaa acttgtagaa cttgcggctc gtgttccatt ttattgacta cacggttgaa
     7861 tgctttcaag atgatttgac tatggtcaaa agccaaagta tctttgccag aagactctcc
     7921 agtgactaaa tcaagcgaga tagccacttc tccgctagat aaatggatct tgttactatg
     7981 acgttctagt gtgaaccagc gtacttcctt ggcatcatct ccggcgatta gcggttcttc
     8041 tccaataaag gctaagtagc tgactgtgac gacccagcct cgcggatctc gattaggcgt
     8101 actgaatgta tgcagttgct caatattttc ttttgaaata accacaccag tttcttcctt
     8161 tgtttctctt aagacgcttt ctccagttga ctcatccttt tggacaaagc caccaggcaa
     8221 tgcccatgaa tttcgataag gatgcccttt tctttggatc aacaaaactt tcagctgatc
     8281 ttcttctttg ttatagcaca tcagtacgat atcgacagtc aaagagggct tgtcatattc
     8341 tggacgttcc tgtttttgat accagttaag aaattcttct tcacttgctt tatgttcata
     8401 atataatttt tcttcttctt tagaagcaaa acggttcatc tgaccacctc cattgacagt
     8461 aaaagtactt tttaccttat tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa
     8521 aatctaggtt atcatttaaa tatgaaaaat aaatggaagt atgagattga ataaaaagaa
     8581 aagcagataa aacggcaata ctttataccg ctcttcacga atggcaaaga agccacaaat
     8641 aacgatatat acgtcaatga taaaacgcaa aatccaagct gctgtcataa aactgttcaa
     8701 atgcccatgg aggttaaaat aaccatacat acgaatataa aaatagctga atattaaatg
     8761 tgctaatgaa agaaaagcaa tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt
     8821 tccagtccct tactaagata gatagtcttt attataccac gtgtgagggg agaaataaaa
     8881 agccgttttt tagtagatat gaaacaaaac aaaaacccgc atttattcaa cctattcatc
     8941 cttttaaagg atagattgaa cgtagataga agtcgtttat ccagtctttt tcattttgaa
     9001 aaaaagctaa cagcatgctt tttttatggt atactagcaa ggaaacgaat tcgcatcatg
     9061 cgataaagga ggaagagccc cgtggcagaa aaggaaactt tttatatcac tacgccaatc
     9121 tattatccaa gcggcaaact tcatatcggc aactcgtata ctacgattgc atgtgacgca
     9181 attgctcgtt ataaacggtt gatgggcttt gatgtgtttt atctcactgg agtggatgaa
     9241 catggtcaaa aaatcgaaac aaaagcagaa gaactaggtg taaaaccgca agaatatgta
     9301 gataaaatgg cggctgatgt caagaaatta tggaaaacgc ttgatatcag ttatgataaa
     9361 ttcattcgca cgacagatga ttaccataaa gcagctgttc aaaaaatatt tgatcgttta
     9421 ttagaacagg gagatattta cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa
     9481 gaattcttta cagaaacaca acttgcagaa gtgtacaaag atgacgaagg taacgtgatc
     9541 ggtggtaaag cgccaagtgg acacgaagta gaattggtaa aagaagaatc atacttcttc
     9601 cgcatgagca aatacgcaga ccgtctattg gaatactatg aagaacatcc cgaattcatc
     9661 cagccagaat ctcgtaaaaa cgaaatgatc aacaacttta tcaaaccagg attagaggat
     9721 cttgcagttt cacgaacaac ttttacttgg ggagtaccag tcaagagtga tccgaaacat
     9781 gtggtgtatg tatggatcga tgcattatcc aattatatta ctgcactagg atatggctcc
     9841 gaagatgaaa gtctattcga aaaatactgg cctgcagatg ttcagatggt cggtaaagaa
     9901 atcgttcgct ttcatacgat ctattggcca atcatgctga tggcccttga tttgcctcta
     9961 cctaaaaaag tatttgggca tggctggcta ttgatgaaag acggaaaaat gtctaaatca
    10021 aaaggaaatg tcgtttatcc tgaaatgctt gtggaacgtt atggtttaga tgcactccgt
    10081 tattatttgc ttcgtgctgt tccgtttggg tcagatggtg tctttacgcc agaagatttt
    10141 gtttcccgtg tcaattatga tctggcaaat gatctaggga acttattgaa tcgaacgatt
    10201 gctatgatca acaaatattg tgatggactc gttcctgact atgcctcact tgtcactcca
    10261 tttgatagtg aactgtctac gacggctgct aacgttgtcg gacgttacca tgatgcaatg
    10321 gaaaaaatgg aattcaatac agcattggct gaaatctggg tgctgatttc tcgtgcaaac
    10381 aaatacattg atgaaacaga accatgggta ttagcaaaag acgaagaaaa gaaagctgaa
    10441 ttgaatagtg tcatgatcca tttagctgaa tctttgcgta tcgcagcgat attacttcag
    10501 ccaatcatga cagaaacgcc agagaagatc ttcaatcaac taggattgga ctctgaaaca
    10561 atgaatcttg aaggattaca ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa
    10621 ggtacaccaa tcttcccacg tttagatatg gaagaagaag ttgcttttat tcaagaaaaa
    10681 atgtctgaag gtactcaaac aaacgaagat acagtcaaat gggatccaga agaaacagaa
    10741 cttgtttcaa cgaaagaaaa acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa
    10801 gtagcagaag tcatcaactg tcaaaaagta gaaggggcag ataagttgct gcaattccga
    10861 ctagatgctg gagacagcca agaccgtcaa atcttgtcag gaatcgctga attttatcct
    10921 gatccaagcg aattgatcgg gaaaaaagtc gtcattgtag caaatctgaa accacgaaag
    10981 atgcgtggac aaatcagtca aggaatgatc ctttctgcag aagcaccaga tggctcttta
    11041 caagtaatcg aagcaccaaa atccatgcca aatggctcag aaatcgcata gaaattcaaa
    11101 gggggtatga cactagttgt gtcatacccc ctttttccga aatttgagaa gcaattttcg
    11161 taaattcctt cttattactt gaagccaatc atataataca gaaacagaga actatccaaa
    11221 ataaatccta gaaaaagaag tacgagcttc gctgattttt gttaatttag aataatacta
    11281 ataggaatcg taaaaacaga aaaaaagcat aatgataaag ggtcgggcag attttaattg
    11341 attcatctgg tactcttttt tattgtcctt acattcataa agtttgaatg caatgacttt
    11401 attacatcta tatttatatc taacagtagc gttgatgtgt taattataag ttctatcact
    11461 ttatgttcaa ttagtttgtg ctcttatatg tgtgattgaa aatatagata aaaaaacaat
    11521 catcggcttt gacttcgatg attgttttct ttattttgta aaaatcctat cacttgattt
    11581 tatggtaacc ttttattttt gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa
    11641 atgggatcga aaagacatac cagaaaatcg tacgtccgat accataatgt tgatagagct
    11701 ttgtcaacat cacgtgtaac aagatcaacc ctaaaagcaa ggagccagct ataaaccaat
    11761 gattatccca atgcatgact tcactaagtg ccatcactcc aaaaggatag aaataaaaat
    11821 agatcccgac taaggtcatc cgccagttct ttgtaaatgt tccgatattc aataaaaaga
    11881 aaatcgctga aagcaaaata ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca
    11941 tcccccacaa gatcatcaca ggacctttga tcaatgcaac gattgcgatg aacaaagtca
    12001 gcaatattag tttttgtgat aacgttgcaa cagtcattaa tccaaataaa attaatgtga
    12061 accaatgcca atgggactgc tgttgttctt tacgggaaaa atcgacccgg ttggataaat
    12121 aatctgctaa tttcggataa gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa
    12181 aggtaggttt agtatacgaa aaataaaaaa gaaatacatc cgtcttaaga gggagttttt
    12241 cactgaaagt ggagaaaaaa gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta
    12301 taccaaattt caaagaagaa agaggatggg aatgaaaggc aaactaaaac gagaaatcaa
    12361 tctgtttggc gcattagcta ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa
    12421 aactgctgct gttactgcta gtactcagtc tacaagtttg acattgctag cttggctttt
    12481 gggtggtttc ttaacgatct gtgctggatt gacagtggct gaattagcta cagcaatccc
    12541 ggaaactggt ggagctgtaa aatatattga agctgcgtat ggaaaattgc ccagcttttt
    12601 attaggatgg gcgcaaagct tgatttactt tcctgctaat attgctgcat tatcgattat
    12661 ctttgcgaca caattgacga acttattaca attatctacc gattatcttt tgttgattgc
    12721 agtgatcacc gctgtttctg tgacagggtt gaatttgtta gggacaaaag tcggtgcttc
    12781 tgtacaatca gtaacgttga ttgttaaatt aataccaatt gctgtgattg tcatatgggg
    12841 attattgacc ccaggacaag gaactgtcca gttatttcca atcgaagctg gaaaagatgt
    12901 tacttttgtc gaaggattga gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg
    12961 gttaggtgtc ggtgcaatgg caggggaaat gaaacggccg gaaaaagatt tgcctaaagc
    13021 aattattctt ggtttgagtt ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt
    13081 gaaaactttg ccgattgatc atcttgccgg taatttgaat gcagcttctg aagcatcaga
    13141 cgtcattttt ggaggaatcg gtggaaagct ggtcactatt gggatcttga tctcggtata
    13201 cggtgcattg aacggttata cactaactgg gatccgcgtg ccttatgcga tggctttaga
    13261 agacgagcta cctttcagta aacagctgac aaagctttca aagaagttta cggttcctta
    13321 tgttcctgcg gttttccagt tagcagtagc ttgtattatg atgagccttg gttctttcga
    13381 ctttttaaca gacatgttga tttttgtgat gtggctattc agtttactga tttttatcgg
    13441 tgtctttatt ttaagaaaaa aagcgccgga attgccacgt ccatataaag taccacttta
    13501 tccaatcgtt ccgattattg cgattctcgg tgctattttc attttaggta tgacgatgat
    13561 gacgcaaacg aaattagcat tgatcggtat aggtgttacc ttgataggta tcccagtcta
    13621 ttatcaaaaa aagaaaaaac gctcagaaga gtaaaatatt tcaacaacag acagaaaaag
    13681 tacgaactag gtcctctaga tcttcgtgct ttttctattt caataaaaca agttcttcta
    13741 ttttgatttt tgctacttct gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag
    13801 agataaacat gatatttgat tctcataccc atttaaatgc tgaacagttc aatgaagata
    13861 ttccagaaac gattcagcga gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct
    13921 tcgatacgcc aacgattgaa aagtctttgc aattaagcca tgactatcca aatatataca
    13981 gtattatcgg ctggcatccg acagaggctg gcagttatac gaaagaaatc gaaaataaat
    14041 tacaagaaca attgacttta ccaaaggttg tagcattagg ggaaattggc ttggattatc
    14101 attggatgga agacccgaaa gaagtccaag ataaagtatt tcgcagacag attgctatcg
    14161 caaaagaaat gaacctacca atcagtattc atactagaga agcacttgaa gacacgtatc
    14221 aaatcctaaa agaagaagat gttcgagata tcggtggaat catgcatagt ttcagtggtg
    14281 attatgaatg ggcaaaacga tttcttgact tagggatgca tatttcattt agcggtgtcg
    14341 tcactttcaa aaaagcatta gacgtccaag aggctgcagc aaatgtacca atcgaacgat
    14401 tacttgtgga aacagatgcc ccttatctgg caccagttcc ttttagagga aagcgaaatg
    14461 aaccaggcta tactcgttat acagtagaaa agattgctga acttcggaat cttccgacag
    14521 aagaagtggc tgcccaaaca tggacaaatg cgcaccgatt atttggtcta agcgaacatg
    14581 gataagatga agatccaaga gattatagtc gttgaaggaa aagacgatac aagaaggctg
    14641 cgggaagtag ttgatgcaga tacgatagaa acaatagggt ctgcaatcaa tgaagagatc
    14701 ctgatgcaaa ttgaacatgc ccaagagact cgtggagtga tcgtttttac cgatcctgat
    14761 ttttcaggag aaaaaataag aaaaatcatt atggaagctg ttcctcaggc aaaacatgct
    14821 tttttgccgc gcacacaagc taaaccaaag aaaaagggaa gccttggtgt cgagcatgca
    14881 agtgatgctg ctattttaga agcattgaaa aaggtcgtga cgccagacat ttcccaaacg
    14941 gctgtttctg aaatcactag acaagacctt ttagactatg gtttaatagc tggggcacaa
    15001 gcaaaagaaa aacgagaaaa actaggtgac gagttgcgca tcggttacac gaacggcaaa
    15061 caattggaaa aacgtttgaa gatgtttcgt attacgccga aagaactcgc ccaagcaatg
    15121 aaaatagtgg aggaacaaca tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa
    15181 agagatttta ttgaaacatg gtttttcatt taaaaaaagt ttaggacaaa attttctgac
    15241 tgaaccaaat attttaagga aaatcgtaga aactgcgcaa atagatgaac atacgaacgt
    15301 gatcgaagtt ggaccaggaa tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca
    15361 agtcgtagca tttgagatcg atgatcgttt gatccctgta ttggctgata cgatgcaacc
    15421 ctatgacaat gtgactattg tacatcaaga tattttaaag accgatttgt caacagctgt
    15481 tagagaaaca tttcatgagg agcttccgtt aaaagtcgta gcgaatttac cttactatat
    15541 tacgacaccg atcatgatgc attttcttga atcagatttg gttgtggatg aactagtcgt
    15601 catgatgcaa aaagaagtag ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg
    15661 ttcattatcg attgctgttc aatattatat ggaagccagt ttagcattca tcgtaccaaa
    15721 aacagtattt gtccctcagc caaatgtaga ttcagcgatt ttgaaactga caagaagaga
    15781 tataccggca gtcgaagtga cagacgaaaa agcatttttc cgattgacaa aagcggcatt
    15841 tcagcaaaga agaaaaacgc tttggaataa cttgcagcat tcgtatggca aagatgacca
    15901 gacaaaagca tggttagcaa aaagcttgga aacagctgga atcgatccaa aacgtcgtgg
    15961 ggagactttg tcattacaag aattcgccgc attaagcaat gcgatgtcag aaaatatgca
    16021 gtaaaaaaag aggttgtgac agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa
    16081 cagccaaaca aaaatagttc ctcatgtttt tcgtttgttt gggcttatta ccgcagatgc
    16141 aagcttttga acaccgttta agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca
    16201 aggaacaatt tcaaaaaaca gcttctcatg tttttgagtt agttggtctt attacttgat
    16261 gcaagctttt gaccactatt atgccgaaca aggagatgtg acacaaattt tgtcacatct
    16321 cctatttttg tttcttgatc aaaaaattct tatacttgat actcacactc attttgtatg
    16381 gatttagaaa agaatatgtg tcttgacagt tattagtaag tgagttatac taagtcgaca
    16441 tttatgtgtc aagacatatt aggagagaga aaaaatgaga aaaaattcag ttcgaaaact
    16501 aacgatttct gctttattga ttgggatggg ggtcattatc ccaatggtca tgcctaaaat
    16561 catgattcca ccagcttcct ttactttggc gtctcatgtg ccattattta ttgcgatgtt
    16621 ttttacacca ggagttgcag tggctgttgc gctaggtacg acatttggtt tctttttaac
    16681 aacgccggtt atcatcgcat tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc
    16741 ttggtacttg caaaagcatc cgagtatcgt tttaaaaaat ggacagttta cttttgctaa
    16801 ttggcgtttc caagggttca attttgtgat cggtgtgatt cattctttag cagaaatgct
    16861 agtagtcagc atcttttact ttattggtaa tatgcctgaa acatattatt ctcaaggata
    16921 tttttataca gtattcatct tgatggggat tggtgggctt atccacagtt tagttgatta
    16981 taatattgct tattttatag caggaacact cagcaaatat tttgatatcc ccgtatttac
    17041 ggcagcaaaa aaagaaaatc ggaaagagtc gattcaaggg tacgcgaaga aaacgacata
    17101 ttagacatga tctctcaaaa aaaaaactct catctagtaa tggataataa atcctctaag
    17161 atgagagttt tttgtcctaa aaagagctgc accaacatct tctggcacag ctctaacagg
    17221 gggcaattta tggaagtttg atgacttgtt ctgtttcttt atcaaagaag tggcctttgc
    17281 tgatgttgaa ggcaaggtcg actatttcgc ctggtttatg gaaatctctt gcatctactt
    17341 ttgagatgaa ctcagtagaa ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt
    17401 cggaaacgac aacttctgat ttcactacgg cttcaggtga agcttcaagg gctacttgtt
    17461 cactgtggat gtcttctgga cgaataccga agataacttc ttttccttca taaccttttt
    17521 caactaatac tttgtttttt ccttcaggaa tacgaagctt aagcccatgt ccatctgtga
    17581 taacaccatt tttcaatgtc acattaaaga agttcatggc aggagatcca ataaagccgg
    17641 caacgaacat attgacgggt gtatcataaa cttctttcgg tgtaccgatt tgttggataa
    17701 atccgtcttt catgataacg attcggtcag ccatagtcat tgcttctgtc tgatcgtgag
    17761 taacatagat ggtcgttgtt tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg
    17821 ctacacgtaa tttagcatct aaatttgata atggctcatc cattaagaag acttttgcat
    17881 cccgaacgat tgcccgtccc aatgccacac gttggcgttg tccaccagat aaagcagctg
    17941 gttttcgttt caagtattct gtcagaccta agatctctgc tgcattgtcc acacgttttt
    18001 tgatgtctgc tttatcatat ttacgtaatt tcaagccaaa agccatatta tcaaaaacag
    18061 tcatatgagg atacaatgcg tagttttgga aaaccatggc aatatcacgg tctttaggtg
    18121 ccacgtcatt catgactttt ccgccgatat ttagttctcc ttcagagata tcttccagtc
    18181 ccgcgatcat tcgcaaagta gttgactttc cacaaccgga aggaccaaca aaaacgataa
    18241 attcacggtc tttgattgat agattaaaat cagttacaga ataattttct gcattgtcat
    18301 attttttata tacgtttttc agagccattt ctaccataat acgttccaca ctttcttttt
    18361 tagttttatt aactatttaa agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg
    18421 aacaagaatt ttttcttttt tttgtcaatg tgctagtttt aagctttaat aaatatccat
    18481 aagaaataaa aactgataca ctggagtaaa taagtatgtt aacaatatca aaaggagtgt
    18541 ctcttatgaa aatcgctttg atcgctcatg accgaaaaaa acctatgatg gtgaaattag
    18601 caacagcgta taaaacaata ttggaaaaac atgaactttt tgctaccggg acgactgggc
    18661 agaggatcac ggaagcaacc ggcttgccga tccatcggtt taaatccgga ccgttaggcg
    18721 gggatcagca aattggagcc atgatttcgg aggatcagtt agatttggtg atttttcttc
    18781 gagacccgct ggctgcacag ccccatgaac cagatgtaac agcacttatc cgtttatctg
    18841 atgtctatga gattccgtta gctacgaata tcggaacagc tgaggtgcta ctaagaggac
    18901 tggaagccgg cttcctagat tttcgaagaa tcgtccatga aatagacaag catccacttt
    18961 cattttgatg gataagcgta tggaaaaata acgtttaatg ttcgcctata tgtagctata
    19021 aaagctaaac aaactgataa tatgcgatat aatacgaacg tattggataa tataaacaat
    19081 aatattagtt tttcgtattt gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa
    19141 gatcccaaaa ataatacaga gtagaaaata aagcgttaag tgctaagagg atgggatgtt
    19201 gcctcttgga cgaaggacgt aatgttcgcg gttttatttt cgcattcgct gcatgatacg
    19261 atgtagcagc tcttaaagga gatgctaaaa atgaagaaac accggttaga cgcacgtatg
    19321 attgctatta tgggcttatt gattgcattg atggtcactt tgtcccgact ggtagcaatc
    19381 gagaccccat ttattaaaat cagtgtcaca tttattccac aagtgatcat gggcattctt
    19441 tttggtcctt tttggtcagg gataggcgca gttttagctg atttagtagg aatggccctc
    19501 ttctcgaaat ctgcattctt tattggattt acattgaatg catttattga aggagcaatc
    19561 tacggattct ttttctatcg aaaagagatc acttggaaaa atgccattct ggctacgctt
    19621 agtgtcacgc tgatcatcaa cttatttttg acaccattat ggcttgcgtt gatgtaccat
    19681 gtgccattgt tcagctgggt cgtttgggcg ccccgcctgt taaaaacagt aatatggtta
    19741 ccgattcaat caattgccat ttattacgta ggacgtagta ttccttacaa aaaaatttta
    19801 agaagtttag cgatccacgc taaataaaaa acaagcagtc ttcccatccc agaagactgc
    19861 ttgtttttat tgtgctgtga attgtgcttg atagagccgt cggtaataac catctttttg
    19921 aagcagctca tcatgggtgc ccatttcgac aatagctccc tgatccatca ctaatatctg
    19981 gtccgcattt ttgattgtgg acaggcgatg ggcaatcaca aaactggttt ttccttccat
    20041 cattgctaag aaggcttgtt ggattttttt ctctgtcaat gtatcgactg agctggtagc
    20101 ttcatctaag atcaacatag ggggattact gatcatcgtt cgagcaatcg tcaacagttg
    20161 gcgttgtcca tctgatatct ttaacccttg accgccaatc ttagtatcta atttttccgg
    20221 caaacgttct acaaattcat acatataaga ggcttttagg gcatcattga tttctgcgtc
    20281 acttgcttcc ggattcccaa aacgaagatt ttctcgcaat gtactgtcaa atagccaagt
    20341 atcttgtaac accattccaa aacttttacg taatgtatca cgttggatag agcggatgtc
    20401 atggtcatcg actgtaattg ttccttttgt tgtgtcataa aaacgcatca gcaaattaac
    20461 aagtgtggat tttccggccc ctgtttttcc tactatagca atggtttgac ctggataagc
    20521 agtaaaattg aaatcttcga tcaaacgctg cttggggtca taagaaaaag aaacatgatc
    20581 aaaagagact tttcctttga tatttttggg ttccaaggca taggcatctg gcaaatcagg
    20641 cttttcttct ggctgatcga tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg
    20701 cagttgtgtc gtgattccag aaagttcgat aaatggttta gaaaactgac ttgagtagat
    20761 cgtaaagctg gaaatcacac caacagtgat tgacgaacca ctcttcaatg ccaataggcc
    20821 gccaactaaa ccaatcgata aataagcaag gtgatctaca aaacgagaag acgggtttgt
    20881 caaggaagaa gaaaattgtg cccgttgtcc tttttcatat agatcagcat ttagcagttc
    20941 aaattgctgt tgactagctt cttcttgctg aaaagctttc acgattttct ggttaccgat
    21001 tcgttcggtc acaaagcccg agatatcccc aataatcctt tgctgtctat cgaaatttgt
    21061 ttgtgaagca cgagcaacga tccaactcac aaggaaaata attggtgtag aacaaagaac
    21121 gaccaatgtc agaagtggac ttagacgcat cataaaaata aacgaaagaa tgatgattgc
    21181 cactccagaa aataattgat tgaaggccgc agaaattgca atggaaatat tgtccatatc
    21241 atttgtaaat cgactgacga tatttccgtg ggagttttga tcataataac gaagtggcaa
    21301 tcgattcaga tgagcaaatg catcttttct taattttgca acggataagt aagctaaccg
    21361 attgcttaac acttggatga gccattgact gactacgttg attaaaacga taccaccgaa
    21421 tagaataagt aaatggaaca gtcgattgaa gcggacctgt ccttctccaa tcatttgatc
    21481 gatactttga ccaatttgta acgtcatcac aactgttgca atcccattga tgattcctag
    21541 tatcacagct agccaaatgt cttttgcata ggcagtaagg taaggaatga atcggcggag
    21601 cgtctgcaag gataattttt ttactttcat aactattctt cctcctgtga tgcgacgatt
    21661 tcctgatatt ctgctgattg agcaagtaat tcttcatggg taccctgagc agcaatctta
    21721 ccttgatcca tcactaagat atgatgtgct tcttgaatag agcggacacg ttgggagata
    21781 ataatcactg tactgccaag ataagtctgt agcgccttac gcaaatccaa gtctgtttga
    21841 taatccaacg cacttaaaga gtcatctaaa atcagtaatg ctggcttggc aatcaaagct
    21901 cttgcaatcg tcagtcgctg tttttgtccg ccagagaaat ttttcccatt ttccatgact
    21961 tttgtatcta aaccatttgg aagctgacgg acaaattctt ctgcctgagc gattttcaga
    22021 gcttcccaac attctttttc tgtggcgtct ttcttacccc attgaaggtt ttcacgaata
    22081 gtcccggtaa atagtacaga agtttgggga acaagagcaa tttgctgacg gagatgatcc
    22141 agctgccaag aacgaacatc cattccgtcc attataactg taccggaact gacatcatag
    22201 aaacgcggaa tcaattgtgt caaagtcgtt ttcccgcttc cggttggacc aatgattcca
    22261 aggactgtat ttcgcttcag ctgaaatgta atattttgga gagaaagacc agaatcagga
    22321 gtataacgga agtcaacatg gtcaaaagcg ataattgctt cagaagaaaa atcgggagta
    22381 gtactgacct cagcttcaag aatcgaattt tctgtgtcta gcacctcttt cacccgattt
    22441 cctgaagctt cggcacgtgt gaagatcacg acgaggtttg aaacaacgat cagtgccaaa
    22501 agcatttgat tcatataatt gatcaaagca agaacttgtc cttgttgcaa gctaccaata
    22561 tttactttga tcccgccaac tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt
    22621 gtagcaggtg ataatagggc agagatattg gaaacacgta aataattgtt cgctagatca
    22681 tttgtgcttt tatcgaactt ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca
    22741 cgaacaccgc taagattctg actaacgtgt tcattgagct gatctagttt ttcttgcact
    22801 ttttggtaga gaggaacggt ttttttgatg ataaaaaata aaatgattga gaaaataggc
    22861 aataatgcta agaaaaacag acctacttcc cagtcgatca caaaagccat gaccactgac
    22921 ccgatactta aaaacggtgc tctgatcaat aaacgaatga ccattgctaa agctagctga
    22981 agctgattga tgtcgtttgt cattcgggtg atcaaggtat ctgttccaaa atgattcaac
    23041 tcgttgtgag agaaagtatt gatttttttg attagctgat tacgtaactc tgtaccaaat
    23101 ccttgtgaag ctactgaagc atagtattgg caaatgatag cacaaatcag accaatcaca
    23161 gacatagcga ccatccataa gcccatttca atgatttttc ctcggtcatt catttttagt
    23221 ccattatcga tcaaggaagc catcattaaa ggcaggacaa gttcgaagac agcttcaaga
    23281 aatttgaaga acgggcccaa aatgatttgc ttacgatagt cttttgcata acgtaataac
    23341 tgaatcatat gattcctcct attatttatt tgtccacaat tgaaaaaaca agtactacca
    23401 cattgtagct taggaaattt agaatgaaaa gagaacaagc cttttcataa atgaaagttt
    23461 tattatgaag aagctttaga aattgaatag actatttttt ttaaaaaaaa gaaaatgaga
    23521 aaacatgcca gtctcttcat tcataggaac gtctatttta cttaattttt ctataaatga
    23581 attctgcata aaaaatatat caagtaaatg tttatagatg aaactctatt tacttctttt
    23641 ttaatttgtt cttgcaatct tttttgctac gtaatttttg taaaaaagtg ggttgagaga
    23701 aatctaattg attttcaaag aaggagtatg aaaatgagaa aaatatttat tcgtttattg
    23761 tgggtactat cttagaggaa taaataatag tggagaaaac tatggtttgg cgcagattct
    23821 atcaggtttg atggaacgaa gacaacctcc cgacatcaag tcaaaagcca cttgagattt
    23881 tgatctgaaa ataaaaaaag aaaacaaggt aggaacatca atgtttctac cttgttttcg
    23941 taatcaatgg gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca
    24001 gtacctcttt actctctgaa agcatcataa ctcgtttttc ctcattatgc caataaattg
    24061 ttgttatacc acatataagg atttgtaata gctaaaaaca gacaggaaag ttgaaatctg
    24121 tgaatcttat tttgtatgga aggatttata gaacgcaaaa atcaagataa agatgaataa
    24181 aaatcctgtt tcggctatat attgcgtatc tacgccaata aaacgagttt gttctgtcat
    24241 agccatataa agcaaaggca aaaatacaac tacagcgaga taagatccga tgatttctcg
    24301 aaaagttttc caggaagtat gtttcctgtt tttatattta tccgatttca ttgaatagtc
    24361 atagcctcct tattctttca tatgttgaaa ttccagtata aaaactggaa ggaactgtac
    24421 gttttttaga ataaaaatta taagggataa gtaggctgct gtctataata agatatattt
    24481 ttcaattctt tattaaagat tttattgaca aaatgaatcc gttttctatg atggaggagg
    24541 ggtgaataag ttgttgaaga aggaaatttt atgttactta gagaaccaga cagccttttt
    24601 cgatttagaa catatgaacg aggtctttac agcacaaagg ttagctgatc attttggggt
    24661 aaaaagaaac acagtcagtc attatttgaa ccagttaaat gatgagcaac tactagtcaa
    24721 aatcaattct cgaccagtga tttatttcca caaagaagct tttgaaaaac aattttttaa
    24781 gttacgcacg aatttttatt tttctatcga tgatctcaaa gaggaacagc cttttttcgc
    24841 gcaaccaaaa gacttattct cttttatgat cggatacgac gccagtttga aagaaagtat
    24901 cgaacaaatc aaaacagctc tttactatcc agatggcgga ttaccacttt tgattacagg
    24961 cgaaagtggc acaggtaaaa gttatttagt taatttggtc tatcagtatt gtctgcttca
    25021 tgatctgcta gaagattctg caccattcat cacttttaat tgtgcacaat atgcagataa
    25081 tcctgaactt ttgactagca atttgtttgg acatgttaag ggagcgtaca ctggtgcaga
    25141 agaaaataaa aaaggggctt ttgaagcagc agatggtggg atcttgtttt tagacgaggt
    25201 acatcgctta acaccagaag gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat
    25261 ttatcgtatg ggagaaccta atttgtcacg ccggatcaaa acacgtctct tttttgctac
    25321 gactgaagag attactagtc atttcttaac aactttcatt cgaagaatac ctatccgaat
    25381 cgaactacct gcacttaatc agcgtagtcg taatgaacgg ctagaactga tttactcttt
    25441 ttttctcgaa gaacaaagaa aaatggggag ccctttgtca gtcagtggac cagctttgtc
    25501 acttttaaca agtcagaaat tatccggaaa cataggagag ctgaaaaata tcgtcaaagt
    25561 ttcagcagca aaagcatatg ctgaacagcg tgaacagcct gaaatccatg tcacgatcca
    25621 tcatttgcaa aaagaactac ttgcgcagcc tattactaaa aatacagcac agcaaggaat
    25681 ctatattacg caagaccaga ccttagagca attaatggag aagcaacagc ccgaacagca
    25741 gcgaatcgtt aaaagttttg agcaaatcct aattgatttc aagaaaaata actgtctgct
    25801 atattcttgc gaaggaaaat taaagcaaga agtgattcaa ttatttgatt ttttgctgtt
    25861 tgagaccagt agacaagaaa aacacgagct gctggtttat tggacacaat ctatccgaga
    25921 gacctttcga caaatggaaa ctgcctatca aatcaaattc gatggaaaca gtgtctatgc
    25981 cttgagttac tatctctatc agcgaagcac tatcaaatgg ttaccggaag aacatgatat
    26041 tatccaacta atcaaagaat tagaacaaca agtttctcag tcttatccat ctagttatca
    26101 ctatgtccaa cgaattctgg aattaagtaa gatcaaatta gatattgaag acacaagtat
    26161 ggatcggatt ttactgacga tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact
    26221 gccaaaagcg atgattgctg ctcacggtta tgcaactgct agcagtatgg caaatgtagt
    26281 aaatcgttta ttacaagagg atctatttga atcatttgat atgccactag atgtcacacc
    26341 acagcagatt gcttcagaaa tccttgatta ttgcgaaaac agcgatgttt caaatggatt
    26401 gatcatatta gtagacatgg gatctctgaa agaaatccat cagtttttta aaaaacagtt
    26461 atccgtaccg cttttgattt tgaataatgt gacgacacct ttagcaatca cagtagggga
    26521 atgcctgcaa aaaaatgtat cattagaaga gatagcagaa gagactgttc gacagatcca
    26581 gccagaatgg cgactgctct atccagagga aaacaaaccc aaagcactca ttactacttg
    26641 cttcactgga atcggtacgg cggtccactt aagtgaactg ctagaaaaaa gcttacctac
    26701 ggcttgtcag ttgaaaatca tcccatatga atatcagcaa ttaaaagata agaaaaacag
    26761 tgacccatta ttttccatat acgaagtcgt aggaatgatt ggcaccacag atcctgatat
    26821 caccaatatc gactatcttt cattagaaga cctcatctcg ggggaaaaga tgtctgtact
    26881 agctgaatgg ttggacacta ctatgaatgc ttcagagaag gaaatattca atcagcgcat
    26941 cattcgtaat ttttcattgg aaaaagtact ggattccgtg actattctag atacagagaa
    27001 ggtgatggga gaaatcgatc attttatgag agaattagag gtgcagttga atcgaagcat
    27061 cagcaatccc aaaaaattag cgttgtatgt ccatgtgagc tgtctcattg aacgattgat
    27121 ccgacaaatg ccaatcgaaa cctatcaagg attagagaaa tggaagcagt gtcaaaaaga
    27181 agggttatct gctattaaat ctgcgtttag tgtcattgaa gagcattata gtgtcatgat
    27241 ccccaattct gagattgcct atatctatga tattttgagt gaagatacag agtttctctc
    27301 gatagaagaa gaattttgaa cataagcaag ttgttgccac taattttggc atgcaacttg
    27361 cttttttagt atgtgtaagt gaaaagagga gatgaaaaag tggatagaga aattatctta
    27421 gcttcccatg gaaaatttgc ttcagggata ttagattcat tagagctgat ctatgggaaa
    27481 aatcattcta ttactgtatt cgattgctat actgatgaga attttgattt agcggaaaca
    27541 gtaaggcagc tttttatgag gtacaaagat aaagaaatca ttgttctgac ggatcttttt
    27601 ggcggaagtg tcaacaacga attcttacaa tatatcaatc gggatcatgt ctatctcgtt
    27661 gccggattga atttgccact attgattgag ctagtatctc ggttggacgc tgaagtagag
    27721 acaactgttt tgatccagca ggtgctaact gattcaaaaa aaatgatcca gttttgtaac
    27781 gagcggctca atcagaaaat agaagaagac gaattttagg aggaagaaaa atgattgtat
    27841 tgacaagagt cgaccatcgg ttattacacg gacaagtagc tttttcatgg acacaaacaa
    27901 taggcgcaga ctgtattttg attgctaatg atgatgtacc tacgaatgag atacgaaaga
    27961 caacgatcaa attagccaag ccacaaggag tcaaactagt gatcaaatca attgatgact
    28021 cgattgccgc gttgaaaagc ggggtgacag ataaatataa gttattcatc gtagtggaat
    28081 cgatcgagga tgcatataag ctggcagaag cgtatccaga aatcaaaacc atcaacttag
    28141 gagggatcaa ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat attttaccag
    28201 aagaggagga acgcttgaaa cgattaattg acaaacaaat agaagtagaa atccgtcaag
    28261 taccagcaga taaaaaagtg gatgtaagag aagttttgta agaaaggaag aacctcaatg
    28321 attgtacaag cagtcttatt aggaattgtt gcatttgtcg cacaatcaga atatgcacta
    28381 gggacatcgt tgctttctcg tccgattgtg acggggctgt ttacaggaat cgtgttaggt
    28441 gatgtaaaaa ctgggatcat tatgggcgca actttagagt tagcatttat cggatcgttc
    28501 tcagtaggtg cttcgattcc acctgatgtt gtgaccggtg ggattttagg gacggctttt
    28561 gctattacag ctggagccgg tacagaaacc gcgcttttac taggtttgcc gattgctacg
    28621 ttgacattga tattgaaaaa tatttactta gggcttttga ttccaattat gaaccataaa
    28681 gcagacaatt atgcagaaga aggaaattat aaaggagtcg agcatatgca cttgcttgcc
    28741 ggtatcggtc tttccttgat gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc
    28801 aatacgatcg gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta
    28861 gctacagggt tgattccagc tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa
    28921 caagttgcac catatttttt cttaggtttt gctatagctt cttacttaga gattcctgtg
    28981 accggagtag ctatttttgg tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc
    29041 cgtcaactac cacttcaaac aactgcaggg gaggtagaag aagatgaaga attctgagat
    29101 caaaaacagc aaaataacga aaaaagagct aaatcacgta ttttggcgtt cttttcaaat
    29161 ggagttttct tggaattatg aacgtcagat gaatatggct tatgtttatg caatgattcc
    29221 gattttgaaa aagttatatc agtcgaaaga agaaatggca gcagctttga aacggcatct
    29281 agagtttttt aacacgaccc cacatatcgt tacactgatt ttaggaatca cggctgcaat
    29341 ggaggaagaa aataaggatg atcctgaatt tgatgtgact gcgatcgata gtatcaaaac
    29401 atctttgatg ggacctcttg cagggttagg agattctttc ttttggggaa ctttgcgatt
    29461 gattgccaca ggtgtgggta cctctttagc catgcaagga aatattttag gccctatctt
    29521 gttcattttg attttcaata ttccgcacat tctgtttcgc tatctagcaa caggttgggg
    29581 atatcgctta ggaacgggtt ttttgaaaaa gatccaggca aacggtatga tggaaagttt
    29641 gacattaggc gcgtctatta ttggactgat ggtcgttgga gcaatgactg ccacgatgat
    29701 caccatcaat attcctttaa aaatcggttc aggagaaaat gctgtgactg ttcaaagtat
    29761 ttttgatgat atcgttccaa atatcttatg tcttggagca tttggcatag tgttttatct
    29821 attgaagaaa gaagtgaaac cattaacgat cttgatcgga ctagctttct ttggaatttt
    29881 tggttcgtgg atagggattt tttaaaaaag gagcagagaa aatgtttacg atgcaagatt
    29941 atatctatga agaaaaggaa gtactaagta cgatcttgaa aaagaatgat ttttcgacaa
    30001 gagaacatat gaaaaaaaca gtgaatttac tgatcttagc aacgggttca tcttataacg
    30061 cttgtttagc agcgaagcca gcattggaat cttatggtga tcttactgtc gatattcaag
    30121 aaccttttta ttttttcaat tatggaaaac tttctccttc aatcgatact gtgattgctg
    30181 tgtcacaaag cgggaaaagt gcttctactg ttgaagcagt aaaaatgata caaaaacaag
    30241 ggttacctgt tgttgctatc acaaatgacg tgcaaagtcc acttgcttta gaagctgcac
    30301 aaattattga tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa ggctattctg
    30361 cgactgtttt acagttacta ctcttaggga taggaattgg aataagtaaa aataaaatca
    30421 gcaaaaaaat agaacaagac tacatgcaac agttgcgcaa aataatcaac catttaccag
    30481 cgattattca aaaaacggaa gaattttttg atggatatca aagtttattt cgtttagcac
    30541 agcgtttcgt caccattgga tatggtccaa actggggaac agctaaagaa gcagagacga
    30601 aattgactga aacaatccgc gttccgtcac aaggatttga gttagaagct tatatgcacg
    30661 gcccatacct ggaggcagat gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa
    30721 ataaggaacg ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga gagactctca
    30781 ccattaccac aaaaaaagca agaaacgaga aaacacttgg attagcaata gaatgtgatg
    30841 aatatctgtc tgtattagcg cttgtcgttc cgtttcaact attcgcttat aaaaccgcag
    30901 tagctaaagg tattgacttg aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa
    30961 gtaaattata agaggtggaa atcatgctga aattcaatga agaagaacaa atcaaagcaa
    31021 taaaaggcgc actagcttta cgtccgcaag tagaagaaat catcgataaa ctttatgcgg
    31081 aaaaatttga cgctgtctat tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag
    31141 ctgtcactta tatgaatggg aaaagcaact tgcctgtctt tgtacagcat gctgcagaat
    31201 attacactac tggaaacaaa agattaacaa aagattctat cgttattttg tcctctgtta
    31261 ccggtacaac tcaagaagta gtgaaagcag tagaacaaat caagaaagta ggagcgacac
    31321 tgattggatt tatcgataaa gcgaacagta agttgtctca gctatgcgat tttgtggtca
    31381 cttatccagc tcctggaaca gaacaaatca aattttttat ggcagcagat cgattgatgt
    31441 atttgaatgg agaattcgaa gcttattcag aatactatga acagctcgaa cgttatttac
    31501 caacagggtt agtggaagca gagaaaaaag cagatgcatt tggcttatca tttgcagaaa
    31561 aacatcgacg tgatagtatg cattatttta ttggagcagg gaatcaatgg ggagctgttt
    31621 attcatatgc catgtgttac tgggaagaac aaagctggct tccatcgaaa tctatccatg
    31681 ctgccgagtt tcttcacggg acgttagaga tcgtagaaga aacaacccca gtcacattat
    31741 ttttaggcga agatgaacag agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa
    31801 tttgcagcaa ttatacattg atagacacaa aagactatcc agtagaaggt atcagtgaaa
    31861 aatatcgcgg acgtgttttg tctttcttat tgatgcatgt agtgacacaa cgcatcgatg
    31921 ctcatgtgga gcagttgaac tgccatccat tagagatacg ccgctactat cgtcagtttg
    31981 attattagga tagactggat aaaaagaata taaaaaataa agagagcagc gataactgct
    32041 ccctttattt ttttaaaata ttggcatcaa ttacgttata aaacacattt tttgcattat
    32101 ctacagttca acttataatc tgtttaatgg gacatgtggg tgccaaccat tttttgttat
    32161 gcaatagtac tatttagttg ttgcatggat ccgtagtaaa agtccaagta caacgagttg
    32221 cgccagttta catagcgttt tgtatccaga atgttgacta aaacaaaagt tcctatgtca
    32281 accgtcaaca gagacaatct gtccgttgca ggacctgaca gatttagaaa tgttcaatct
    32341 gtctcttatg tattttaaat caatactcac taggagaagt ttcctaacct cctaatagta
    32401 atgctggtag taacattgtc aaaactgctt cctcttagga acaagcaaag cataacgagt
    32461 gtaaaaataa aatgtagact attgtaacat atcattgatt atttcaagta aaaacgataa
    32521 aagaagtaaa caaacactta aaaacaatgg aaattagtcc caactttaag aaatgtatac
    32581 tataatggaa attatcaatt atttatttgg aggaatatcc atgaaacaaa aaatcgcagt
    32641 tcttggtcct ggatcttggg gcactgcttt agcacaaaca ttggcagaaa acgggcatga
    32701 tgtccgtatt tggggaaatg tacctgaaca gattgatgaa atcaataagt atcatacaaa
    32761 tcagcatttt ttacctgatt tgacgatccc agaatcgatt atcggatata aagaattggc
    32821 agaagcagtg gataacgcag atgctatttt atttgttgtt ccaacaaaag cgattcgttc
    32881 agtcgcacag gaacttattg gtaaaatgaa tactaagcca atcattatac atgcaagtaa
    32941 aggactagaa caagatacgc ataaacgaat ctcagaagtt ttggctgaag aaattccaga
    33001 agaaaaaaga caggcaattg ttgtattatc tggtcctagc catgctgagg aagtggcagt
    33061 acatgatatc acaacaatta cagctgcaag tattgatcaa aaagcagcag cctatgtaca
    33121 aaaactgttc atgaatgatt atttcaggat ttatacaaat aatgatgtga ttggcgtaga
    33181 aactggtgca gcactgaaaa atatcattgc aattggtgcc ggcgctatcc atggattagg
    33241 attcggagat gatgcaaaag cagcaatcat gactcgagga ttggcagaaa tcagccgtct
    33301 tggtgtcgcg atgggtgcca atcctctgac atttatcggt ttgagtggtg tgggggactt
    33361 gatcgttact tgtacaagtg ttcattctcg caattggcgt gccggtaact tgttaggaca
    33421 gggacataag ctggaagaag ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac
    33481 gacaaaagca gcagtagagc tagctcaaca gctaggtgtt gagatgccaa ttactcaaac
    33541 aatctataat gttttgtata atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt
    33601 acgtgacgga aaaatggaga atgaattttc tcttcgttaa atagatgatc aatagttaat
    33661 cgtcgtttag gaggaacagc aagtgaaggt caaaaaagca gtcattcctg ccgctgggct
    33721 aggaacacgt tttttgccgg caacaaaagc catggcaaaa gaaatgcttc caatcgtgga
    33781 taaaccaacg atccagttta ttgtggaaga agcattggat tcgggaatcg aagacatctt
    33841 gatcgtaacg ggaaaagaaa aaagacccat tgaagatcac tttgatgcaa atatcgaatt
    33901 ggaaaacaat ttgcgggaaa aggaaaaaac agaattactc gctttagttg aagaaacgac
    33961 tcaagtcaat cttcacttca tccgtcaatc tcatccaaga ggactggggg atgccgtgct
    34021 tcaagcaaaa gcatttatag gaaatgaacc gtttgtcgtc atgctggggg atgatttgat
    34081 gaaagatgat atcccattga ctcaacaatt gatgatggat tatgaagaaa cacaagcttc
    34141 cgctgttgcc gtcatgcgtg taccagaaaa tgaaacatct aaatacggaa ttattgatcc
    34201 taaaacagat agcgaaaagg gtcgttgcag agtaaaaggg tttgtagaaa aacctgaaat
    34261 gggaaaagca ccaagtaatt tagccattat cggacgttat ctactgactc ctaaaatatt
    34321 tgagatatta gaaactcaag aaccaggagc aggaaacgag atacagctaa cggatgcact
    34381 tcagactctt aaccagacag aggcggtcta tgcgagagag tttaaaggaa aaagatacga
    34441 cgtaggcgat aagttaggat atatgaagac aaatattgaa tatggacttc aacattcgga
    34501 aattgggact tcattaagtg catatattat ccagttgagt aaaaagctaa gaacagaaag
    34561 ttaactggaa ataagtggcc tgctgagaat gaaaggctat ttgttattaa agcggcagct
    34621 tgtgcgtcat ttgcgcaact gtcgttttag ttttttgact ctagatcagt tgagtcgtgg
    34681 aaaacgaaag atagaaaaag actttcgtga atagattcag aaaatattat gctaatggtt
    34741 taattttttt ttaagttgta taaaagaaaa tgattacata agagaaatga aagccaagcc
    34801 agaagattca tagtagaata aaaaggcgaa ttttttaatt ataaaaaaag gtgtgtgttc
    34861 actatgggaa aattagtcgc tgttgcacag tcgctgcttg atatactcaa gtggctgatg
    34921 gaaggaagca attggatatt tgtgttgcca ctattgctta ttagtctatt tatcatgttt
    34981 cgcatggtca gaaaaaaaga aacgtttctg atttttatct ggtatgcagc atccgttggg
    35041 gttgctacct tgatggttga attggcgtta ggtagttggg aagcttgcgt atttgcttta
    35101 ttttttgctg tactgtatct aatcatttat atcccatata tgaaaagaaa gtctcggata
    35161 ggcagaaaag gtagacatac aactactttt tctagtacag attctctcga acaaagtaga
    35221 agaacgagaa gaagacggta atatttagta ggtatacgca aaaaaagata aaataatgga
    35281 taaactattg agatgagaga tttatttaat tggtggaaaa aagctttttt cacttatata
    35341 aaagaatct
//
LOCUS       DAZAJZ010000017        34960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000017 DAZAJZ010000000
VERSION     DAZAJZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.017"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG86D_000856"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG86D_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN3140024.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG86D_000857"
     CDS             complement(2020..2922)
                     /locus_tag="ACG86D_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3140025.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG86D_000858"
     CDS             complement(2919..3611)
                     /locus_tag="ACG86D_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN3140026.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG86D_000859"
     CDS             complement(3654..4295)
                     /locus_tag="ACG86D_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN3140027.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG86D_000860"
     CDS             complement(4333..4647)
                     /locus_tag="ACG86D_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140028.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG86D_000861"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG86D_000861"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFN3140029.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG86D_000862"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG86D_000862"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN3140030.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG86D_000863"
     CDS             complement(5984..6916)
                     /locus_tag="ACG86D_000863"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN3140031.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG86D_000864"
     CDS             complement(6913..7689)
                     /locus_tag="ACG86D_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN3140032.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG86D_000865"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG86D_000865"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN3140033.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG86D_000866"
     CDS             complement(10933..12009)
                     /locus_tag="ACG86D_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN3140034.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG86D_000867"
     CDS             complement(12014..13294)
                     /locus_tag="ACG86D_000867"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN3140035.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG86D_000868"
     CDS             complement(13313..14239)
                     /locus_tag="ACG86D_000868"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN3140036.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG86D_000869"
     CDS             complement(14252..15535)
                     /locus_tag="ACG86D_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN3140037.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG86D_000870"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG86D_000870"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN3140038.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG86D_000871"
     CDS             complement(16323..17234)
                     /locus_tag="ACG86D_000871"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3140039.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG86D_000872"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG86D_000872"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN3140040.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG86D_000873"
     CDS             complement(17700..18398)
                     /locus_tag="ACG86D_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN3140041.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG86D_000874"
     CDS             complement(18385..18771)
                     /locus_tag="ACG86D_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140042.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG86D_000875"
     CDS             complement(18918..20588)
                     /locus_tag="ACG86D_000875"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN3140043.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG86D_000876"
     CDS             20771..21004
                     /locus_tag="ACG86D_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3140044.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG86D_000877"
     CDS             complement(21103..21534)
                     /locus_tag="ACG86D_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN3140045.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACG86D_000878"
     CDS             21613..22026
                     /locus_tag="ACG86D_000878"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN3140046.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACG86D_000879"
     CDS             complement(22064..23212)
                     /locus_tag="ACG86D_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3140047.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACG86D_000880"
     CDS             complement(23334..23930)
                     /locus_tag="ACG86D_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3140048.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG86D_000881"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG86D_000881"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN3140049.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACG86D_000882"
     CDS             27160..27591
                     /locus_tag="ACG86D_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3140050.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACG86D_000883"
     CDS             complement(27636..28985)
                     /locus_tag="ACG86D_000883"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3140051.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACG86D_000884"
     CDS             complement(29055..30218)
                     /locus_tag="ACG86D_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3140052.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACG86D_000885"
     CDS             30347..30670
                     /locus_tag="ACG86D_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140053.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACG86D_000886"
     CDS             complement(30853..32202)
                     /locus_tag="ACG86D_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3140054.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACG86D_000887"
     CDS             complement(32271..33224)
                     /locus_tag="ACG86D_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN3140055.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACG86D_000888"
     CDS             complement(33326..34516)
                     /locus_tag="ACG86D_000888"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN3140056.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34960)
                     /locus_tag="ACG86D_000889"
     CDS             complement(34716..>34960)
                     /locus_tag="ACG86D_000889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140057.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9951 a         7641 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DAZAJZ010000018        34749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000018 DAZAJZ010000000
VERSION     DAZAJZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.018"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..631)
                     /locus_tag="ACG86D_000890"
     CDS             complement(107..631)
                     /locus_tag="ACG86D_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN3140058.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG86D_000891"
     CDS             complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG86D_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN3140059.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG86D_000892"
     CDS             complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG86D_000892"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN3140060.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2353..3033)
                     /locus_tag="ACG86D_000893"
     CDS             complement(2353..3033)
                     /locus_tag="ACG86D_000893"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN3140061.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3159..4028
                     /locus_tag="ACG86D_000894"
     CDS             3159..4028
                     /locus_tag="ACG86D_000894"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3140062.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4054..4569
                     /locus_tag="ACG86D_000895"
     CDS             4054..4569
                     /locus_tag="ACG86D_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN3140063.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4616..5179)
                     /locus_tag="ACG86D_000896"
     CDS             complement(4616..5179)
                     /locus_tag="ACG86D_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3140064.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5234..5413)
                     /locus_tag="ACG86D_000897"
     CDS             complement(5234..5413)
                     /locus_tag="ACG86D_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140065.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5490..5999)
                     /locus_tag="ACG86D_000898"
     CDS             complement(5490..5999)
                     /locus_tag="ACG86D_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140066.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6095..6475)
                     /locus_tag="ACG86D_000899"
     CDS             complement(6095..6475)
                     /locus_tag="ACG86D_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3140067.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG86D_000900"
     CDS             6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG86D_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN3140068.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7738..9015)
                     /locus_tag="ACG86D_000901"
     CDS             complement(7738..9015)
                     /locus_tag="ACG86D_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140069.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9094..9738)
                     /gene="pcp"
                     /locus_tag="ACG86D_000902"
     CDS             complement(9094..9738)
                     /gene="pcp"
                     /locus_tag="ACG86D_000902"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN3140070.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9937..10881)
                     /locus_tag="ACG86D_000903"
     CDS             complement(9937..10881)
                     /locus_tag="ACG86D_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN3140071.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10883..11569)
                     /locus_tag="ACG86D_000904"
     CDS             complement(10883..11569)
                     /locus_tag="ACG86D_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN3140072.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12043..12363
                     /locus_tag="ACG86D_000905"
     CDS             12043..12363
                     /locus_tag="ACG86D_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN3140073.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12517..13422
                     /locus_tag="ACG86D_000906"
     CDS             12517..13422
                     /locus_tag="ACG86D_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140074.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13419..14192
                     /locus_tag="ACG86D_000907"
     CDS             13419..14192
                     /locus_tag="ACG86D_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3140075.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14373..15797)
                     /locus_tag="ACG86D_000908"
     CDS             complement(14373..15797)
                     /locus_tag="ACG86D_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN3140076.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16008..16454
                     /gene="nrdI"
                     /locus_tag="ACG86D_000909"
     CDS             16008..16454
                     /gene="nrdI"
                     /locus_tag="ACG86D_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN3140077.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16519..18501)
                     /locus_tag="ACG86D_000910"
     CDS             complement(16519..18501)
                     /locus_tag="ACG86D_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN3140078.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18515..19411)
                     /gene="murQ"
                     /locus_tag="ACG86D_000911"
     CDS             complement(18515..19411)
                     /gene="murQ"
                     /locus_tag="ACG86D_000911"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN3140079.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19414..20475)
                     /locus_tag="ACG86D_000912"
     CDS             complement(19414..20475)
                     /locus_tag="ACG86D_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN3140080.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20686..21213)
                     /locus_tag="ACG86D_000913"
     CDS             complement(20686..21213)
                     /locus_tag="ACG86D_000913"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3140081.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21280..22782)
                     /gene="eat(A)"
                     /locus_tag="ACG86D_000914"
     CDS             complement(21280..22782)
                     /gene="eat(A)"
                     /locus_tag="ACG86D_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN3140082.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23040..24161)
                     /locus_tag="ACG86D_000915"
     CDS             complement(23040..24161)
                     /locus_tag="ACG86D_000915"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3140083.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24287..25228
                     /locus_tag="ACG86D_000916"
     CDS             24287..25228
                     /locus_tag="ACG86D_000916"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3140084.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25378..26274)
                     /locus_tag="ACG86D_000917"
     CDS             complement(25378..26274)
                     /locus_tag="ACG86D_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN3140085.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26543..27883
                     /locus_tag="ACG86D_000918"
     CDS             26543..27883
                     /locus_tag="ACG86D_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3140086.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28101..28544
                     /locus_tag="ACG86D_000919"
     CDS             28101..28544
                     /locus_tag="ACG86D_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN3140087.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29048..30466)
                     /gene="nhaC"
                     /locus_tag="ACG86D_000920"
     CDS             complement(29048..30466)
                     /gene="nhaC"
                     /locus_tag="ACG86D_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN3140088.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30869..32092)
                     /locus_tag="ACG86D_000921"
     CDS             complement(30869..32092)
                     /locus_tag="ACG86D_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3140089.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32123..32962)
                     /locus_tag="ACG86D_000922"
     CDS             complement(32123..32962)
                     /locus_tag="ACG86D_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3140090.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32979..33659)
                     /locus_tag="ACG86D_000923"
     CDS             complement(32979..33659)
                     /locus_tag="ACG86D_000923"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN3140091.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33661..34734)
                     /locus_tag="ACG86D_000924"
     CDS             complement(33661..34734)
                     /locus_tag="ACG86D_000924"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN3140092.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
BASE COUNT        10016 a         7252 c         6197 g        11284 t
ORIGIN      
        1 tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc
       61 tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat
      121 aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga
      181 ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt
      241 gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt
      301 ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt
      361 ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg
      421 tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact
      481 cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg
      541 tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt
      601 cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc
      661 tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa
      721 tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat
      781 agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa
      841 tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga
      901 ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct
      961 tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga
     1021 ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat
     1081 cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg
     1141 cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg
     1201 gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt
     1261 tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt
     1321 gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat
     1381 ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg
     1441 gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga
     1501 tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt
     1561 ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat
     1621 cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg
     1681 gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca
     1741 tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg
     1801 ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca
     1861 tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt
     1921 tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt
     1981 aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat
     2041 ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc
     2101 gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac
     2161 ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt
     2221 ttatatgtat tatcatacac tattccagaa aaaaataaca gtttatttta tctgtatcag
     2281 tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa
     2341 agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc
     2401 aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga
     2461 taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat
     2521 tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa
     2581 tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg
     2641 cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac
     2701 tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt
     2761 tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg
     2821 attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt
     2881 caattccaat gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc
     2941 tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac
     3001 ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata
     3061 actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt
     3121 aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag
     3181 atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga
     3241 tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag
     3301 aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg
     3361 cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag
     3421 caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg
     3481 atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta
     3541 cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt
     3601 tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag
     3661 ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc
     3721 agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga
     3781 accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt
     3841 ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag
     3901 caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata
     3961 caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag
     4021 acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag
     4081 cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg
     4141 atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat
     4201 ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa
     4261 gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat
     4321 attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat
     4381 catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat
     4441 gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta
     4501 gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac
     4561 ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga
     4621 tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga
     4681 agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga
     4741 tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga
     4801 tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa
     4861 acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag
     4921 gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg
     4981 caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat
     5041 cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg
     5101 gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt
     5161 ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga
     5221 aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag
     5281 taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat
     5341 tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt
     5401 tcattttttc catgttatct caacttcctt tcatctttac tctcttagct tactcttata
     5461 ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt
     5521 tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac
     5581 tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt
     5641 tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca
     5701 tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa
     5761 aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga
     5821 atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat
     5881 gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc
     5941 gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt
     6001 cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga
     6061 atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc
     6121 cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt
     6181 ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga
     6241 aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa
     6301 ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt
     6361 ctttgcggta attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg
     6421 atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag
     6481 cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg
     6541 tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa
     6601 agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat
     6661 attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta
     6721 gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt
     6781 tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta
     6841 aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg
     6901 actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt
     6961 ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt
     7021 ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt
     7081 gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat
     7141 cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat cgcaatcatc
     7201 agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct
     7261 cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc
     7321 ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt
     7381 tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt
     7441 tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt
     7501 gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac
     7561 tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     7621 ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     7681 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttttta
     7741 tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt
     7801 ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca
     7861 tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt
     7921 ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg
     7981 caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt
     8041 aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg
     8101 ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt
     8161 ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc
     8221 atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc
     8281 tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt
     8341 ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata
     8401 atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt
     8461 tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt
     8521 ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa
     8581 ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg
     8641 caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt
     8701 aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg
     8761 ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa
     8821 ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc
     8881 ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt
     8941 agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga
     9001 ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat
     9061 aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga
     9121 aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc
     9181 ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg
     9241 cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg
     9301 atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc
     9361 atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg
     9421 accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc
     9481 ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc
     9541 gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct
     9601 tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg
     9661 catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc
     9721 tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt
     9781 ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa
     9841 cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg
     9901 ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc
     9961 aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt
    10021 gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag
    10081 cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc
    10141 atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc
    10201 attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt
    10261 cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc
    10321 gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac
    10381 tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa
    10441 gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa
    10501 attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat
    10561 tttcattcct agtttgtctg acattttttc catatattct tttgatggaa gccggctttc
    10621 tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt
    10681 cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa
    10741 ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat
    10801 caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc
    10861 aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt
    10921 tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa
    10981 cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt
    11041 ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat
    11101 tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc
    11161 cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa
    11221 ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta
    11281 caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca
    11341 gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc
    11401 gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag
    11461 tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa
    11521 acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc
    11581 cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt
    11641 ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc
    11701 ttacttattg taaggtaaag ctttttttaa tttattgccc gataatatat agaaataagg
    11761 tctagttact gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa
    11821 agcattcatc ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa
    11881 aagtatgaga ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc
    11941 tgccaccggg taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc
    12001 ctctttttgt gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta
    12061 atcatagcag gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt
    12121 tctaaaatca attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg
    12181 tcaaaggcaa tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc
    12241 gcagttggtt cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact
    12301 tggttctttg tggggatgct actcatcagt atcattggca tcaagattac tagcggccac
    12361 tagacacttg gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt
    12421 ttttatgaaa aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt
    12481 atgcttgtac tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa
    12541 ttatccaaga actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct
    12601 ggcaaaatcg ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct
    12661 attttaggac tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct
    12721 ccctctagtc ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat
    12781 ccattcttaa caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct
    12841 atcgacacta tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc
    12901 tactcgttgg gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct
    12961 atcgttattt tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga
    13021 aaaatcatta ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc
    13081 agcgagcttg aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa
    13141 caatgtacaa tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca
    13201 aatgatctag ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat
    13261 aaaatcaagc ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct
    13321 gcacaaaata tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta
    13381 ttggaaattc tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag
    13441 aattatttaa attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt
    13501 tgctcatagg aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa
    13561 tgactgctca gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa
    13621 gcacgatcat ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt
    13681 attctcgagg agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt
    13741 tatacctttt agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt
    13801 ccttcacgga gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt
    13861 tgggtagtta tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta
    13921 tcaatagttc cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca
    13981 tatctggtat ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca
    14041 gtatgttgaa tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc
    14101 tttctactaa tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag
    14161 gatatctcgt atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag
    14221 agacgttcag ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga
    14281 gctgtgacaa catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc
    14341 tcacaacctc tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat
    14401 aaaaccattg caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg
    14461 atatgttgta caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc
    14521 aagaaaagaa caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact
    14581 tgaaaggcac ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa
    14641 agtttcattt ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt
    14701 gtgtaagcaa agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta
    14761 cataacgtcg tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat
    14821 gctttaggca aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc
    14881 catgagagcc aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc
    14941 catttaccaa ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc
    15001 agctgattca tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc
    15061 aagccgaata tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa
    15121 cctacgacag aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc
    15181 aatacttgac tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa
    15241 aagttatcaa tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat
    15301 aatggcacta atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc
    15361 cgattcacta gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga
    15421 ttttgcccac cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca
    15481 aaagctacgt tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg
    15541 aaacggccaa aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt
    15601 ttattgatat tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa
    15661 ctgaaaatag ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca
    15721 cctccaatcg ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc
    15781 tgttgctctg tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata
    15841 acaaaccttt tttattttta cttaattaat aaaaacttta atagaaatat aattctaccg
    15901 gcttttttct cattatacaa gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa
    15961 acttaaaaaa gatgctatac taaaaggaaa gaattggagg atgatccatg aacattttat
    16021 atatatcgat ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa
    16081 aaatgcatgc agaagatcct gtcaatcctg aagttacttt aaaagaaatc catgaaaata
    16141 gtgattttac gaaagaaaca gaaccatttt ttactttcgt tcctacttat ttagacggcg
    16201 gaaatggttt agataacggt gatacagaaa tcttgaccga aacgatgcgt gaatatctgg
    16261 aacatgaaga caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca
    16321 ataatcagta ttgtctaaca gccaaacaat atgcacaaac atttggcttt cctttcttgg
    16381 cagattatga attacgggga acaccatctg atgtgacacg aatttatcag attttagcag
    16441 aaaacaataa ataataaaaa agaagaagct gaaaaataga tttcagtttc ttctttcgtt
    16501 tctattgttt ctatcatatt aagctttgac atccatcaca ggtgcttttg cttttgcttt
    16561 acctgttttt tccaagacaa agttttttac acgatccata ttggttacca agacgatcat
    16621 ggatgtttct ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta caagcgtatc
    16681 aggtgtcact tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac
    16741 tgtgttgatt cccatatgaa gcaagatttc taaaccattt ggcgttgtga tcccaatagc
    16801 gtgtttcgtt gggaagacag agctgactac cccagtaact ggagaataga tatgtccgtt
    16861 ttctggttct acaaagtagc cttcacccat catcttttgt gcaaaaactg gatcacttgc
    16921 tttatctaaa gaaacaactg ttccgtcagc aactgatgag aggatagtca cactttcagc
    16981 tgaagtttga ggtactactt tttcaggcac ttcatttact gtggctgttt cagtcgctaa
    17041 ttcttctttt tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc
    17101 agcataagct gccaataatc caaggatgta gcctaaccat ttaccgtcag agatcaatgg
    17161 gatcaaagcg gctcctgaag gtccaatagc gatagccccg acatttccga tcattccgat
    17221 cactgccccg ccgatacctc caccgataca agcagtaata aatgggcgtc ctagaggtaa
    17281 tgttactccg taaatcaatg gttcgccgat tcctaggatc ccgactggta aagctccttt
    17341 gatcatttct gttaattctt tatctttttt gcagcgaatc cataatgcca atgctgcacc
    17401 cacttgtcct gcaccagcca tcgcaagaat tggaagcaat agcgtacgac cagtttcatc
    17461 aatcatttgc acatggattg gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa
    17521 gaataatgtg cctagaacga agccggcaaa tgctccacca acgccaagta cccagttgat
    17581 cccaccgacc atgccatcag aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat
    17641 cagaccgatg gcaatcaatg aaagagttgg tgtaacgatg atatctaccg catcaggtac
    17701 tactttatgc agttttttct caacgattga caatagccaa acagcgaaga tgacacctag
    17761 gatcccacct tgtccagctg caagagcttc accagtaaat aagtttttca gtggtgcttc
    17821 cggattcatc cctgtcagca tcacaacggc cccgataact ccaccaagag ttggtgttgc
    17881 accaaaaact tgtgcagcat tgatacctgt atagataact aggtagctaa acatcccgtt
    17941 tttcaagatg ttcatcacat cgatatattg ttgccatgtt gctgcatcaa gtgttccggc
    18001 tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg atcccagtac cgacaaatgc
    18061 aggaatcatt ggcacaaaga tattagaaat gtcttttaat accgatttga ttttagatgg
    18121 tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt
    18181 tccagatgct gatgaatttg ctgtagctgt acttacagaa gaaagttttt ctcctaattt
    18241 cacgcctgcc aaatcaacca tttgctgtgc tactttgttc actttcccag gtccgatgat
    18301 gacttgaagc tgttcatctt ctactacacc aagcactcct ggaatcgctt ttaattcaga
    18361 gacatttaca agatcatcat tgatgatctt catacgcaca cgagtcatgc agtggacaat
    18421 gctttctaca ttgcccatgc cgcctgcccc tttataaatt tgttttgcca gttcttccaa
    18481 agtcagttcg ttttttgcca tggtcttttc cccctcataa ggtccctttt acaaatccgt
    18541 ttgcttcagc taattttgct tgagcttcct ctttatcact atttgtcaaa atcatgacga
    18601 tagccagctt aacattttga tctgcttctt caaagtactt ttcagctgtt tcgtaatcag
    18661 cttctgtcgc ttgcatgatg attcgttttg agcgttcgat tagtttttca tttgttggtt
    18721 tgacatcgac catcaagtta ttgaatactt tacctatacc aatcatactg atagtagaaa
    18781 tcatattcaa gataagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa
    18841 attctggacc tgcatcgact tcgattggga aatccgcgtg tttactgatt tcggcttgtt
    18901 tattgcaaga aatcgttcct gtgacagcac cgatcgattt tgcgtaatct aatccaccga
    18961 tcacatatgg tgtacgaccg ctagctgcga taccaataac tatatcattt tttgtcaaat
    19021 tcagcttgat tagatcttct tgccctagct cataagaatc ttcggcccct tcgacagcta
    19081 cagtcattgc ttcttctcca ccagcaatta gacctatgac catttccggt tctacaccga
    19141 atgtcggtac gcattcagca gcatctaaca cacctagtct tccacttgtt ccggctccca
    19201 tataaatcag acgtccacct tttttaaagg actcaatcgt gttagcaatc actggttcga
    19261 tacttgtcag ttctttttca accgcagcgg ccactttctg atcttctttg ttcattagtt
    19321 gaacggcttg agaaatactc atctcatcta atccaaaagt ttcttggttt ctgcgttccg
    19381 ttgttaggtt ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg
    19441 atagttcata ccagctttga tttggttgat caatggtagc tcttctttga taactttagc
    19501 tactacattc actttttcat ccgctggcaa atcatatttg gttaattgga tctcacccat
    19561 atagcgcaaa tacttttcgt tatctaaagt aatagaccct ttcatacgcg tagctgtatt
    19621 tcttgctggg ataggcggaa tctctcgaaa acgtgcatct acgcttcgta tgacatctct
    19681 tgcatcgtct tgccgattcg tatgcttccc taatatatat tcgtaaaatt cttcatctac
    19741 aggttccgta tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac
    19801 gttttctgac agacctgcat ctccgatgta aacaatatcc gtgttacttg ctaatagatc
    19861 tagagcgcct gcaagaggat ggatccctcg atgttcttcc aaagttggca atccttgata
    19921 tagtgggcct ctcaactctg tatctcctgg tacaaaacct tggacaagga gaccttgaat
    19981 tttgagccat tgattttttt tctgatacca ttctttgtcc agccctgttt cgggacgtgg
    20041 ataataatta tgccaagctt caatctgtga gaaatcagct tctgcttctt tcaattcatc
    20101 gatatcttta ggcgtgatcg tgctagcatt caaactgatt ttcaattgat gtgaccagtc
    20161 ggcaatctgc tgattggaaa tatgataatc cattcttaaa cctgttacac caattgcttt
    20221 caaaggcgtt agatcttcta atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc
    20281 gaccatcaat tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgcttttg
    20341 atatgccgaa acatcatctt ctggtatatg catggaagtg aaaataccga caaagccatt
    20401 ggatgccatt tggcggatat actgtttctt ctcttctgac aaagaatcgt tcataaatat
    20461 tgaaaagcca aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag
    20521 tatatatcta aagaaacaaa atttcaatac ttttattaaa ataattaaat ttcatttcta
    20581 aaggttgagt ttatattatc aaaagaattg tcataaccta aaaaaggcac tgctaagcaa
    20641 aagcagtgcc ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac
    20701 caattctgta ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata
    20761 agcattgatc actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc
    20821 ttcgatgcat tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc
    20881 atttgggaat cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg
    20941 cagtccatca taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa
    21001 gctgagacga tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat
    21061 gattttcgtg attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat
    21121 ctgtcctttt tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca
    21181 agttgtttct ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt
    21241 ccccttaacc ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat
    21301 aatctcagta gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg
    21361 tttcacgttc aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa
    21421 tggttcatcc catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt
    21481 tttccgttgg cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc
    21541 aagctttcgg aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc
    21601 cgccaacgtt cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt
    21661 tggctggtat ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat
    21721 ggatgacttt cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt
    21781 aaaatgaatt ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa
    21841 aagtcgctta tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc
    21901 ttttaatagt ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga
    21961 tcgtttcatc gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc
    22021 agtatcgatg aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc
    22081 ggctttttca gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg
    22141 agccatttca aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg
    22201 ataaagttcc agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa
    22261 tccccggtca tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac
    22321 ttgttttcta ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt
    22381 gtcatccaca aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct
    22441 gaattctctc cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc
    22501 ccatatctca aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt
    22561 tgtctgttgc gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag
    22621 tttgttttgt agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc
    22681 tagtttccat tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct
    22741 gtcgtagccg aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa
    22801 aaaagaccag tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa
    22861 aaagacccat acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt
    22921 ctccacaagg tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg
    22981 atccctcact ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac
    23041 tacttcggga ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct
    23101 aatgtttgcc tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag
    23161 acaattggga gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg
    23221 attattggtt ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg
    23281 atcgtttgct gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt
    23341 ttttccaatc cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac
    23401 aaaaaaaggg ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc
    23461 acctcccaga aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct
    23521 agcgtcgtat ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct
    23581 gtatatggat tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt
    23641 cttcgatcaa aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct
    23701 aaagaagcac ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct
    23761 aaagtgacaa taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag
    23821 gcataactgg aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc
    23881 aatgcttgtg cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag
    23941 ctgatttttc gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg
    24001 agcgcgtaat agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt
    24061 tttcttcctt tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa
    24121 gcacttcctt taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt
    24181 cttaagtaag acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt
    24241 ttctatatac taaaagggaa ctcattgaat aaaggagaga aaaaacatga aaatcgcaat
    24301 agctggggcc ggagcaatgg gcagccgaat cggattgatg ctacatcaaa gcggaaatga
    24361 ggtcctcttg atcgatcgtt ggcctgctca cattgaagcc attcgtacaa atgggttgat
    24421 tgctgacttt aacggtaaag aagtggtcgc aaaactgcca atctattcac cagaagaaat
    24481 catcgaaagc aacgaacacg tggacttgat cgtagcatta acaaaagcca atcaattaga
    24541 tgatatgttt tgttcgatcc aatccattat tactgacaat acttatgttt tgtgtttact
    24601 aaatggatta ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa atatcttatt
    24661 tgggatcacg atgtggacag ctggtctagc tggtcccgga aaagtcacat tgttaggtga
    24721 tggagaaatc gaactagaga acttggaacc agaaggcgaa gcatttacaa aaaaagttgt
    24781 agaagtcttt caagaagcaa atttaaatcc gatctatagc cataacgttc gttattccat
    24841 ttggcgaaaa gcttgtgtaa atggaacatt gaatggttta tgcacgattt tggattgtaa
    24901 tattgctgaa ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa tcgtatctga
    24961 atttgcttct atcgcagcaa aagaaggaat catactagat caagaagaag tgtaccaaca
    25021 tattgcttct acttatgatc ctgacaacat cgggttgcat tacccatcta tgtaccaaga
    25081 cctgatcaaa aaccatcgtt tgactgaaat cgactacatc aatggtgcca tttggcgaaa
    25141 aggacaaaaa tatgatattg ctacaccata ttgtgccttt ttgacccaat tagtccatgc
    25201 aaaagaagga atccttggcg cagaataaaa ggctctctga tgagaatcag ctgaatcagc
    25261 agctggttct ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat
    25321 gccataagaa ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt aggtttttta
    25381 tttgttttca acagaggaga ggtcatattc gtattgctca taaatagtgg aatcgttagt
    25441 gataaatcgt tttgcccaat tttctgcatc ataaaattcc aatagctctg ataggattgg
    25501 cttaaatggt gtatgcagaa attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa
    25561 aatattttca ggacgataaa aatgataatg agcaatcgct tgattatccg tcactagttt
    25621 cttttgaaaa tacatggatt caaatgtccg caaagtcact ccttgctgac cctcccgcaa
    25681 cagttccaga atcccgcgcg attcccctgt ttttttcagg tactcttcat aagtaagata
    25741 tccaggttgt tcattccctc gatgtggaag aatgaaatag ttggtcgtaa catctctttt
    25801 tataaattcc tgcttatagc tttctgctcg ttcttttctc ccattattcg ttgcaccaaa
    25861 aaatagatcc gtctttggtt cgctagaaga aaaattcata tgtttcgaat aaaatgaact
    25921 attatgtttc agcccaagtt cttttgcttc caaaaaatca aaatgataaa tctcatcgat
    25981 tgccggttca tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga agtgattcca
    26041 aaaaaataac acaatttttg cattggtaaa tgggcgcacg cgtcgagcca tctccatatc
    26101 aaatccataa tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc tagctgattt
    26161 aatgaattcc gactgtagta aatcttttcc ggaccaaaca tctaaagagc ggatggaagg
    26221 caaaaaataa agtgatttat tttttgcaac tagcagtacg tcatttttgt tcatcttttc
    26281 cgcctcactt tctctgtcac tttttcctat gatagcctgt ttaaatagta aaaagccagt
    26341 aaaacgtttc ctatatttgt atgaaaattt aacatttgta tgaaaatatg atgaaataaa
    26401 tcattcttct attaaaattc gcctaaaagt tttctgaata gttgtaattg tctgaaaatt
    26461 tgttatcatt agtcattgtt aaatattgct atttattctt aatataaatt ttgattgtga
    26521 atttaaaaga aagaggtcat ttatggaaga taaaaaattg aaaaaacaac ttagctccag
    26581 acatatcacc atgttagctt taggtggagc aatcggtgca ggtctattta aaggaagtgg
    26641 cgaagcgatt gggatcgccg gcccttctgt attgattgct tttttgattg gtgggctgat
    26701 tttatatatc gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg acacgcatca
    26761 cggattatct ggtttgatac gcccttatct aggtgctcat tctgctgatt ttactgattg
    26821 ggtctactgg tctatgtgga ttatcaatat catcgctgaa gctgttgcag ctgcttcctt
    26881 tttgcagttg tggttcccgc acattccagc ctgggtattt gtttttatgt tggctgtttt
    26941 aactacgatc atcaatcttt attcggtacg gttattcgca gaaacagaat attggctggc
    27001 ttttgctaaa atcagtgtca ttatcttact gatcattttt gctacttatc ttgtcggtca
    27061 gcaaatgttg ggcacaggcg ttttccctac ccttcagcaa ttgactgacc atggtgggtt
    27121 tactcctcat ggtatgaagg gaatcatcag ttctttgctt gtagttatct attcttacgg
    27181 cggttctgaa ctgattgcta tcacggtaag cgaagcagat gatcctaaac gtgcgattcc
    27241 taaagcaatc aaaggtgtca tgggaagaat catttccttt tacatcattc ctttgttttt
    27301 gcttttgatt ctttttcctt ggaatacttt agcaggcacg aatgtcagtc cttttgtgat
    27361 ggtatttgaa aaaatgaata tcccatttgc agcggatatc gtcaattttg tgattgtcct
    27421 tgctttgttc tcatctatca attcgggtgt gtatgcatct tcaagaattt tgttcttccg
    27481 tttgaaggat cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc atcaagtacc
    27541 gcaacgtgct gttttatttt gtaccagtgt tctttatctt ggtgtagctc tttcttattt
    27601 tgtcggtgac aaattatttg gctatctggc aggttccctt tcgtatacag tattattgat
    27661 ctggatcatt atcagtgcgg catcctttgt actggcacta agaaaaggaa cattatggga
    27721 taaaggaatc agcttttttg cattagctgt cttaggattg attttccttg gtatcctgtt
    27781 cacgaactcc attggggtaa ctgtattgac tggcctgctt tacctgttca tttatttcag
    27841 ttatcaaaaa aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat agaaaatttt
    27901 attttattta tgtggaatag atggagctat tgaagaaagg agttgtgaca aaagtcttgt
    27961 tgcaactcct ttttccgaat aaacagtctc atgacctctt ggtaaacggt gttcaaaaac
    28021 cctctgtgca aaataattga ttgttctcca tgctcatgac atgatagaag aaaggaatga
    28081 aaaaaaggag tttgttttaa atgaaaaagt attggaagtt tttgttgatg atcggtgttt
    28141 caacggtagt tatgtttggt atgatgtact tgaatgtcta tgcgctcgat catatcttct
    28201 tcagtcgtac acgagtattc atggcgttga tgatgggtgc agtcatggca gtcatcatgc
    28261 tgttgttcat gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc ttagccgcta
    28321 gtcttcttgt ttttgccggt tccttgttta tggtacgaag ccaaactact gtcaacgaca
    28381 cagcttggat gaaggccatg attcctcatc attctatcgc tatcctggca agtaaacgag
    28441 ctgatttaaa agatcctgaa gtaaaaaaat tagcggaaga aatcattcag gcacaagaag
    28501 aagagatcga caaaatgaaa aaaatgatca aagaaatgga atgaccatta tccgtccaaa
    28561 taaaaaaaga ggatgttcca gaagttatca gcttcaagaa ataagaagaa gctacttctg
    28621 gaacgccgtg aataaggggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca
    28681 aacaaaaata gcttctccta tttttcgttt ggttaggctt attaccgcag atgcaagctt
    28741 ttgaacaccg tttattaaga ggttgtaaaa tcagcgttct gacctgagta ttagagagac
    28801 aaatggaaaa atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg
    28861 attgaacacc gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa
    28921 attttgtgaa atggctttcc acatttttgt ataaatttgg cttaataccg agaaactggc
    28981 ttttgaatac cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac aatccctttt
    29041 tatgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt
    29101 tttttgagat tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac
    29161 tgaaaaatac gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac
    29221 ttccggcaat tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc
    29281 tacctaatac agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag
    29341 gcagaataac agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca
    29401 atgttgtaaa aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg
    29461 ttgagatcaa gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg
    29521 acacagtagg catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt
    29581 ttccagtatt tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt
    29641 gtgggttttg aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa
    29701 ttgccggcac ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga
    29761 ttgaaatagt aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg
    29821 cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga
    29881 cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg
    29941 ctagatttgt tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa
    30001 tagctccaac aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa
    30061 tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca
    30121 caacaaatac agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta
    30181 gagttggaat gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa
    30241 taaagattgg gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg
    30301 tcgggtatct ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga
    30361 tattaggtga gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta
    30421 atacaagtgt tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt
    30481 ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat
    30541 ccgcgattcc tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc
    30601 gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt
    30661 atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt
    30721 ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc
    30781 ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta
    30841 taactttcac acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg
    30901 ctactatttc ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat
    30961 caagagctgt attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac
    31021 ttttagcagc agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta
    31081 ccgtaggctg gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac
    31141 ttgttctttg ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga
    31201 agaataagtc atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc
    31261 caaactcaaa agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt
    31321 catatacttt atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt
    31381 aatctggaat gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat
    31441 cttttggcaa ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca
    31501 aagcatttac tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga
    31561 tgtcgactgg atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga
    31621 cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg
    31681 atccgttctc gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt
    31741 catcaatgaa ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat
    31801 gcccgcatgc atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg
    31861 gaccgggttt attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt
    31921 tttctaccca aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact
    31981 catagttact tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta
    32041 aacgatattg gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt
    32101 cctcctatgc tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg
    32161 atccttttga tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta
    32221 ttttttgata cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag
    32281 gacgtgcatg gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag
    32341 catctacagc tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg
    32401 tttgggaagc atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa
    32461 cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca
    32521 gaagagctct gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga
    32581 tatcagctac atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc
    32641 cgatcgatac taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt
    32701 ggaaagaatt caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg
    32761 taaatttcac gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc
    32821 atacatctgt atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg
    32881 tatctgcttt tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta
    32941 caaaacgaat aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc
    33001 agttgattac tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca
    33061 atcgttacat ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg
    33121 cctgcaccta ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc
    33181 gagacccgaa tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt
    33241 ggactggttc ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg
    33301 ttctcgattt gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct
    33361 ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag
    33421 ataatgaaag ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga
    33481 cggttagcta gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca
    33541 atcatcccgc caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg
    33601 acaaattcat ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt
    33661 ttatcttcct ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt
    33721 tatttttaca ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc
    33781 agctaaagta acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata
    33841 taaaatattc gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt
    33901 ctgatttcca atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc
    33961 agaatgggca atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc
    34021 tttggtcaat tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat
    34081 tcttccatct tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg
    34141 agtgatcagg acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat
    34201 ctgaagggtc gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg
    34261 ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt
    34321 aggataagca gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg
    34381 acgacgttcc tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat
    34441 agttcggctt tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt
    34501 tcgcaattct ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc
    34561 tgttggtttc tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta
    34621 accgacgatt ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg
    34681 tatctctttt tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat
    34741 tcctcctta
//
LOCUS       DAZAJZ010000019        34429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000019 DAZAJZ010000000
VERSION     DAZAJZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.019"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(260..997)
                     /locus_tag="ACG86D_000925"
     CDS             complement(260..997)
                     /locus_tag="ACG86D_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3140093.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(999..2501)
                     /locus_tag="ACG86D_000926"
     CDS             complement(999..2501)
                     /locus_tag="ACG86D_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN3140094.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2760..4754
                     /gene="uvrB"
                     /locus_tag="ACG86D_000927"
     CDS             2760..4754
                     /gene="uvrB"
                     /locus_tag="ACG86D_000927"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN3140095.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4764..7580
                     /gene="uvrA"
                     /locus_tag="ACG86D_000928"
     CDS             4764..7580
                     /gene="uvrA"
                     /locus_tag="ACG86D_000928"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN3140096.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7843..9108
                     /locus_tag="ACG86D_000929"
     CDS             7843..9108
                     /locus_tag="ACG86D_000929"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN3140097.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9112..9843
                     /locus_tag="ACG86D_000930"
     CDS             9112..9843
                     /locus_tag="ACG86D_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN3140098.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            9978..10862
                     /gene="rapZ"
                     /locus_tag="ACG86D_000931"
     CDS             9978..10862
                     /gene="rapZ"
                     /locus_tag="ACG86D_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN3140099.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            10859..11857
                     /gene="yvcK"
                     /locus_tag="ACG86D_000932"
     CDS             10859..11857
                     /gene="yvcK"
                     /locus_tag="ACG86D_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN3140100.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            11891..12826
                     /gene="whiA"
                     /locus_tag="ACG86D_000933"
     CDS             11891..12826
                     /gene="whiA"
                     /locus_tag="ACG86D_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN3140101.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            13394..14050
                     /locus_tag="ACG86D_000934"
     CDS             13394..14050
                     /locus_tag="ACG86D_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140102.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            14229..14794
                     /locus_tag="ACG86D_000935"
                     /pseudo
     CDS             14229..14794
                     /locus_tag="ACG86D_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15493..16014)
                     /locus_tag="ACG86D_000936"
     CDS             complement(15493..16014)
                     /locus_tag="ACG86D_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN3140103.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            16212..19892
                     /gene="nifJ"
                     /locus_tag="ACG86D_000937"
     CDS             16212..19892
                     /gene="nifJ"
                     /locus_tag="ACG86D_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN3140104.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            20081..20968
                     /gene="cdaA"
                     /locus_tag="ACG86D_000938"
     CDS             20081..20968
                     /gene="cdaA"
                     /locus_tag="ACG86D_000938"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN3140105.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            20965..22134
                     /locus_tag="ACG86D_000939"
     CDS             20965..22134
                     /locus_tag="ACG86D_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN3140106.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            22158..23513
                     /gene="glmM"
                     /locus_tag="ACG86D_000940"
     CDS             22158..23513
                     /gene="glmM"
                     /locus_tag="ACG86D_000940"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN3140107.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23585..25390)
                     /gene="glmS"
                     /locus_tag="ACG86D_000941"
     CDS             complement(23585..25390)
                     /gene="glmS"
                     /locus_tag="ACG86D_000941"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN3140108.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            25800..26090
                     /locus_tag="ACG86D_000942"
     CDS             25800..26090
                     /locus_tag="ACG86D_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140109.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26103..27128
                     /gene="sppA"
                     /locus_tag="ACG86D_000943"
     CDS             26103..27128
                     /gene="sppA"
                     /locus_tag="ACG86D_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN3140110.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            27131..27790
                     /locus_tag="ACG86D_000944"
     CDS             27131..27790
                     /locus_tag="ACG86D_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN3140111.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            27947..28879
                     /locus_tag="ACG86D_000945"
     CDS             27947..28879
                     /locus_tag="ACG86D_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140112.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            29033..30322
                     /gene="tig"
                     /locus_tag="ACG86D_000946"
     CDS             29033..30322
                     /gene="tig"
                     /locus_tag="ACG86D_000946"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN3140113.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            30569..31819
                     /gene="clpX"
                     /locus_tag="ACG86D_000947"
     CDS             30569..31819
                     /gene="clpX"
                     /locus_tag="ACG86D_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN3140114.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            31902..32489
                     /gene="yihA"
                     /locus_tag="ACG86D_000948"
     CDS             31902..32489
                     /gene="yihA"
                     /locus_tag="ACG86D_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN3140115.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(32563..32718)
                     /locus_tag="ACG86D_000949"
     CDS             complement(32563..32718)
                     /locus_tag="ACG86D_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN3140116.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            32854..34182
                     /locus_tag="ACG86D_000950"
     CDS             32854..34182
                     /locus_tag="ACG86D_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140117.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            34193..34402
                     /locus_tag="ACG86D_000951"
     CDS             34193..34402
                     /locus_tag="ACG86D_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140118.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        11249 a         5949 c         7441 g         9790 t
ORIGIN      
        1 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat aagataatca
       61 tcatagtttc tgaaggtggt atcgactatg tacttgatat cgtcaatgaa ccgatggtac
      121 tttcttatgc gatggaacag gtagagcgat tgcttgtttg tgcaggagaa caagcgatgc
      181 ggctggtaca gttaagtgaa aaaacaagaa actaaaaatg cccggtcgtc atgaagcgac
      241 cgggcatttt tggtttttat taaatattta atactttatt caagaaatct tttgtccgtt
      301 cattttgcgg attttcaaag atttgttgag gcgttccgtc ttctaaaaag tttccgtcgt
      361 caatgaacat gacacgattg gccacttctt tagcgaagcc catttcgtgc gtaacgataa
      421 ccatcgtcat accttgttct gctaactttt tcatgacgtt caagacatca ccgaccattt
      481 cggggtcgag tgcagatgta ggctcatcaa aaagcatgat gtcagggttc atcgctagag
      541 cacgagcaat agccacacgc tgcttttgtc caccggaaag catttcagga tacatatctt
      601 ttttatcttc tagaccaact gtctgcagca atttgattgc ttttttctct gcttcttctc
      661 tagattcttt cttcaggtcc atcggtgcta aaatgatgtt ttccaaaata gaaagatgag
      721 ggaataggtt aaagtgctgg aataccattc cgatatgttg tctcaccaag ttgatatctg
      781 tgcttttatc catcaagtgg gcgccgtcaa taataatttc accgtcagaa ggttcttcta
      841 aacgattcaa gcaacgcaaa aatgtacttt ttcctgaacc tgaagggcca atgatacaga
      901 cgacatctcc ttcgttgatg gacacattga tgtcgtttaa tactgtgtta tcgccgtatt
      961 ttttgactaa atgctcaact aagatttttt ctgccatctt acttcacctt cttttctaaa
     1021 acttttgcta attttgtcaa tgctgtgatc aaaatcaagt acatgatcgc aatgatcaag
     1081 taaacatacg tactttgtgt tgtacgtgca acaatgatct taccagtttg cagcaattcg
     1141 actacaccga ttgcggaaat gatcgttgtg tcttttaatg agataacgaa ctgattgacg
     1201 aatgatggga tcatgatgcg tatcgcttgc ggcaaaatga ttttttgcat tgtccgatta
     1261 taagaaagac ctaaacttct ggaagcttcc atttgtccga caggaacggc gttgatcccg
     1321 cctcgaacaa tctctgcgat ataagcactt gcgttaagag tcaaggtgat aacaccagca
     1381 aggaagtcag gaatcgtaaa tccaacgata ccaggcaacc caaagaaaat aaagaacgca
     1441 agaaccatca ttggaattcc tcggatgata tcgacataaa tactagcaat tgtgcgaaga
     1501 gcgcgtattg gtgctacact gaataatcca aaaatcacac cgattactat agccaaagca
     1561 aaggagatca aagttaggag aattgttctc caaagtcctt tcaacaatgc ttgatagttg
     1621 ttttggataa gtccaagaaa tgttgattca tcagcagtgg aagactcact ggaagtttct
     1681 gaaccatccg ctacatattc tttaataatc ttatcgtatt cgcctgttct ttttaattct
     1741 ttcaatcctt cattgaacat attaagaagc tcaggatttt gtcctttttt aacggcaaat
     1801 gcatattttc cgccagattc ttttttgatc ggtgtttcca attcttgtcc ttgtttgatc
     1861 gcataaccga ttaccggata atcatccatc attgcatcga ttgcgccaac ttttaacccg
     1921 tcgtacaatt gatcagcagc atcaaaatat ttgatagaat agccatattc agattcatgg
     1981 tcgcttaaaa attcagcact ttcggtacca acttttgctc cgactgtttt tcctttcaaa
     2041 tcttcataag acttgatcga agtatctcct tttttgacag ctaattggat cccgctatca
     2101 aagtaagaat cagaaaaatc atagcttttc tttcgttcat ctgttacagt cattccggca
     2161 atcattccgt ctgcttgtcc tgattccaaa gcttgcaaag cagcagagaa accacggtag
     2221 tccattcgga tgttaaatcc ttgcagtttg gcgatgtttt ccatcaattt tacatcgatg
     2281 ccttcgtact cgccctttgc gttttgaaat tcaaatggag caaaagcaga gtcactggca
     2341 attacgtata cgtctttttt gggctctatt ttttgcattg tctcgcttgt gcttgactct
     2401 gccgttgtag tgttgtcagc ttgtgcaaca tgtccaaacg caagtgtcac aagtcctgtc
     2461 attaagaaaa caaatgagtg aaataaatgc tttcttttca taatcaacct cctaaatttc
     2521 caatatttta ttattatagg cgaaaagtgc tgaatcagac aagactgtaa gctcgaaaaa
     2581 atgaaaaaaa tgcgagtttc taacatttta tgttataaaa cgtaacatgc ataataagat
     2641 aaaaaagtgg cgaaagtatg ttcggtgtgg tataattttt tagtaaggaa tgcctttgct
     2701 ttccttttct ttttattggt cgtaaaatga aaaggaatga atgaaaagga ggacgaagaa
     2761 tgattgagag agatacctcc cgacactttg agcttgtctc gaaataccaa ccggctggtg
     2821 atcagcctga agcgattaac caattagtgg atggcgtagt cggcgggaaa aaagcacaaa
     2881 tcttattagg tgcgactgga acagggaaaa catatacgat ttccaatctt attgagaaag
     2941 tgaataaacc aacactaatt attgcccata acaaaacatt ggctggtcag ctgtacgggg
     3001 aattcaagga atttttccct aacaatgctg ttgaatattt tgtttcttat tatgattatt
     3061 atcagcctga agcttatgtg ccttcaagcg atacgtatat cgaaaaagat tcaagtgtaa
     3121 atgatgagat cgacaaatta cgacattcgg ctactagttc attattagaa agaaatgatg
     3181 tgatcgtcat tgcatccgtt tcatgtattt ttggattagg ttcgcctttt gagtaccaaa
     3241 aacaagtagt ttcgattcga cagggagcag aactagatcg caatcagttg attcgtgact
     3301 tagtcagtat ccaattcgaa agaaatgata tagattttca gcgcggtcgt tttcgagtgc
     3361 gcggtgatgt cgtggagatt tttccggctt caagagatga acgtgcctta cgtgtggagt
     3421 tttttggaga tgaggtcgaa cgtatccgag aagtcaatgc actgacaggt gaagttttag
     3481 gagaaacgga acacgtggca atcttcccgg cgactcactt tgtgacgaat gatgaacata
     3541 tggagcatgc cgtagcgaat atcaaagcgg aattagaaca gcgtttaact gtcttgcgaa
     3601 atgaaaacaa acttctggaa gctcaacgtt tagaacagcg aaccaattac gatattgaaa
     3661 tgatgttaga aatgggctac acgtcgggaa ttgaaaatta ttctcggcat atggacggac
     3721 gtaaagaagg tgaaccgcct tatacgctat tggatttctt tcctgaagat tttttgatcg
     3781 tggcggacga atcgcacgta accatgcctc aagtcagagg aatgtataat ggtgaccgag
     3841 cgagaaaaca gatgctagta gattacggtt tccgtttgcc atctgcttta gacaatcggc
     3901 cacttcggtt agaagagttt gaaaaacatg tcaatcagat catttatgtc tctgcgacgc
     3961 caggaccata tgaacatgaa caaacagata ccgttatcca acaaatcatc cgtccgacag
     4021 ggctgcttga cccagtgatc gaagttcgtc caatcatggg acaaatcgat gatctagtag
     4081 gcgaaataaa tgaacgagtc gaaaaagacc agcgggtgtt tgtaacgaca ttgacgaaaa
     4141 aaatggcaga agacctcaca gattatttca aagaattagg gatcaaagtc aaatatttgc
     4201 atagtgatat caaaacgctg gaacgaacag aaatcattcg agatcttcga ttaggtgaat
     4261 ttgatgtgtt agttggaatc aatttgcttc gtgaaggatt agacgtacct gaagtttccc
     4321 ttgttgctat cttggatgcg gataaagaag gctttttacg tagtgaacgt tccttagtcc
     4381 aaacgattgg acgagctgct cggaatgaag aaggtaaagt aatcatgtac gcagacaaag
     4441 taacagattc tatgcgtcta gcgatggatg aaacatcaag acgccgtacg attcagcaga
     4501 agtacaatga agaacatggg atcgttccaa aaacgatcat taaagaaatc cgagatttga
     4561 tttctattac gaaagaatcg gaagatgata caaaagaagc ggtacaagtc tcgtatgaag
     4621 aaatgacgaa agaagaaaaa gatacgttat tgatgaaatt agaaaaagaa atgaaaaatg
     4681 cggcgaaagc attggatttt gaaacagccg ctaatgtgcg agatatgatt ttagaattaa
     4741 aagcttcaaa gtaggaggac aacatggcta acgataaaat tacgattcac ggtgcacgtg
     4801 cccataactt gaaaaatata gatgtaacga ttccacgaga taaattcgtg gtcgttacag
     4861 gtttgtccgg ttctggaaaa agttccctag catttgatac gctgtacgct gaagggcagc
     4921 ggagatacgt agagagcctt tctgcttatg ctcgtcagtt tttaggacaa atggataagc
     4981 cggacgtaga tagcatcgac ggattgagtc cggcgatttc tattgatcag aaaacaacaa
     5041 gtaaaaatcc tcggtcgaca gtaggtactg tgacggaaat caatgactat ttacgcttgt
     5101 tgtttgcacg tgtaggacat ccgatttgtc ccaacgatca tatcgagatt accagtcaat
     5161 cagtagatca aatggcagat caagtaatgg ctttgcctga gcgcacaaag atccaagtgt
     5221 tggcaccgat cgtggtaaag aaaaaaggac aacataaaaa aatattcgaa cgaatccaaa
     5281 aagagggata cgtccgcgtt cgagtcgatg gtgagatcta tgatttaagt gaagtgcctg
     5341 agctggagaa aaacaaaaaa catgatatcg cgatcgtcat cgaccggatc attgtgaaag
     5401 aagggattcg ttctcgtcta tttgattctt tagaggcggc tctgcgattg gcagacggct
     5461 atgctctggt tgacgtgatc ggtgaagaag aaatgttgtt tagcgaacac tatgcctgtc
     5521 cttattgcgg atttactgtt ggcgaactag aaccaagact gttctcattc aatgcgcctt
     5581 ttggagcttg tccagactgt gatggtttag gtgtcaaatt agaagtcgat caagatttag
     5641 tgattccaga tagaacgaag acacttagtc aaggtgctat cgttccatgg aatccaatca
     5701 gctctcagta ttatcctcaa atgttagcgc aggcttgtga aagtttcggt atcgatttag
     5761 atacaccatt cgaagaatta ccggaagagc atcaggatat catcttgaat gggacagatg
     5821 atcttttcca tttccattat gaaaatgatt ttggtggcgt tcgcgatgta gaaacgactt
     5881 tcgaaggggt attgaagaat atcgagcgtc gttatcatga tacgaacagt gatttcacgc
     5941 gtgagcaaat gcgtctttat atgacagaat taacttgtca gacatgtaaa ggttatcgat
     6001 tgaatcccca agctctatct gttcaaatca atggaacgaa tatcgggcaa acaaatgagt
     6061 tgtctattca aaaagcagta gatttcttct caaacttaac gttatctgaa caagagaaag
     6121 taattgccaa accgatttta aaagaagtaa atgacagact ttcattcttg gaaaatgtgg
     6181 gactagacta tctaactttg agtcgcgctt ccggaacatt atctggggga gaagcgcagc
     6241 gaatcagatt ggctacacaa attggatcga atctttctgg agttttgtat attttagatg
     6301 aaccatcgat cggattgcat caacgcgaca acgatcgact gattggctca ttaaagaaaa
     6361 tgcgtgatct tggcaacacc ctcgttgtag tcgaacatga tgaagatacg atgtgggcag
     6421 cagattatct gattgacgtc ggacctgggg ccggagaaca aggcggacaa atcgtggctg
     6481 caggcactcc tgaagaagta gctaatgatg aaaattcttt gacagggaaa tatctttcag
     6541 ggaaaaaatc aatccctgta ccaaaagaac gccgtaaagg taatgggaaa gcaatcaaga
     6601 tcactggtgc ttcagaaaac aatctgaaaa atattagtgt tgaatttcca ttaggagaat
     6661 ttgtagcagt cacaggtgtg tctggttctg gaaagagtac attggtcaat agtatcttaa
     6721 agaaatcgtt agcgcaaaaa ttaaataaga attctgctaa gccaggtaaa ttcaagacaa
     6781 tttccggcta cgaaagtatc gaaaagatca tcgatatcga tcaaagccca atcggccgga
     6841 cgccgagaag taatccagcg acttatacaa gtgtatttga tgatatccgt gggttatttg
     6901 ctcaaacgaa cgaggcaaaa atgcggggtt ataagaaagg gcgttttagt ttcaacgtaa
     6961 aaggcggtcg ttgtgaagct tgtcgcgggg atggaattat taagatcgaa atgcactttt
     7021 tgcctgatgt ctatgttcct tgtgaagtat gtcatggcaa acgatataac tctgaaacat
     7081 tagaagtgca ttacaaagga aaaagcattg ctgatatttt ggaaatgaca gtagaagatg
     7141 ctgtagaatt cttcaagcac attccaaaga ttcatcgcaa actgcaaacg attgttgatg
     7201 ttggcttagg ttatgtgacg atggggcaac cagcaacgac attgtccggt ggtgaggcac
     7261 aacggatgaa acttgccagt gaattgcaca aaatctctaa tggaaagaat ttctatatac
     7321 tagatgaacc aacgacagga cttcatagcg atgacatcgc ccgcttgttg catgtattac
     7381 aaagattagt agatgctggt aacacagttt tagtgattga acacaatcta gatgtaatca
     7441 aaacagcaga ttatatcatt gatttaggac cagaaggtgg agaaggtgga ggaacgatcc
     7501 ttgcgactgg aacaccagaa gaaatcatta acgtaaaaga aagttataca ggtcactatt
     7561 tgaaaaaaat aatggtataa agaggataga tagctgagat agttaaaaaa acataaaaaa
     7621 atgaattttt gcttgtttta ttaatatcag tgttctagaa tcattacatg gtagttcttt
     7681 ctacactgat tggtatagac aaaaacttcc tataagttca atccgttcaa aaaagaattg
     7741 agcaatcgga ggagtttcct ttgattgctt ttttctttat ggtatgatat tccttgtgta
     7801 gttggaaaag aaaaacaaga gattatgagt aagtgagaga taatgatgaa cagtattgaa
     7861 aatgaagaat ttatccctat attattagga agcgacatga acgtttatgg tatggcacgt
     7921 tcttttaatg aagcatacgg caaaatctgc caagcatatg cttcggatca gttagcccct
     7981 acccgctaca gtaaaattgt caatgtagaa gttattccag gatttgataa agaccctgtg
     8041 tttatcgaga cgatgcttcg tttggcaaaa gaaagatatt ctgataagag taaaaaatat
     8101 cttttgattg cttgtggtga tggatatgca gaattgatct cccaacataa acaagagctg
     8161 tcagaatatt ttatctgtcc atatattgat tattctttat ttgaaagact gatcaataaa
     8221 gtaagctttt atgaagtttg tgaagaatat gatcttccgt atcctaaaac attgatcgtc
     8281 agagaagaaa tgttagtaaa tggacatttg gaacaggaat tgccttttga attcccagtt
     8341 gctttgaaac ctgcaaacag tgtggagtat ctttctgtcc aatttgaagg aagaaaaaaa
     8401 gcttttattt tagagactag agaagagttc gatttaattt taggccggat atacgaagcc
     8461 ggttataaaa gtgaaatgat tgtacaagat tttatcccgg gtgatgatag taatatgcgt
     8521 gtgctaaatg cgtatgtaga tgaagatcat caagttcgga tgatgtgtct tggtcatcca
     8581 ctgttagaag atcctacacc agcatcgatt ggtaattacg ttgtgatcat gccagattat
     8641 aacgaaaaaa tctatcaaac cattaaggca tttttggaaa aaatcgaata tacaggcttt
     8701 gccaatttcg atatgaagta tgatcctaga gatggagaat ataaattatt tgaaatcaat
     8761 ttgagacaag gtcgtagcag tttctttgtg acattgaacg gcttgaatct ggctcgtttc
     8821 gtcacagaag atcgagtgtt taacaaaccc tttgttgaaa cgacctatgg aacgaatcaa
     8881 tcagataaag caagattatg gatgggtgtg cccaaaaaaa tctttttaga atatgctcgg
     8941 gaaaatgaag acaaaaaact agctgagcaa atgatcaaag aaaatcgtta cggtactact
     9001 gtgttttatg aaaaagaccg gtcaatcaag cgctggttgt tgatgaaata tatgttccat
     9061 aactatattc caagattcaa aaagtatttt catgtgaaag aaggctagtt catggagaat
     9121 tttttcagta ttttaggcgg aatgggcacg atggcgacag aaagttttgt tcgcttgatc
     9181 aaccaccgaa cgaaagctac aaaagatcaa gaatatttga attatgtctt attcaatcat
     9241 gcaacagttc ctgatcgtac agcttatatt ttagatcgat cagaagaaaa tccaatgccg
     9301 tttctgctgg atgatattga gaaacaaaat ttattgcagc cgaacttcat tgtgttgact
     9361 tgtaacaccg cacactactt ttttgaagaa ttacaagcgg caacggatat tcctatcctt
     9421 catatgccaa gagaagcagc aaatgaactt gtccgtcagc atacgacagg gagagtagcg
     9481 attctaggaa cagaaggaag tatgaaagct ggaatctatg aacgcgaagt caaaaatctt
     9541 ggattcgaaa cagtgattcc tgatactgtt cttcaagaaa aaatcaatta tctgatctat
     9601 catgagatca aggaatcgga ttatttgaat caagagctat attacgaaat cttagaagaa
     9661 gcggtagagc gcctaaactg tgaaaaagtt atcttaggtt gcacagaatt gtcattgatg
     9721 catgaatttg ctgaagataa ccattatcca gtgatcgatg cccaatctat tttagcagat
     9781 cggacgattg aacgagcgct agctgaaaga agcaaggcat tggatacagc atcggaaaag
     9841 taaaaaaatt ggatttgggg caaatatcct aaaagatatt tggtccaaat ctttttgtaa
     9901 aagaaaaaga aaactggtaa tgatacgccc ggaatgatat aattttacta tgcacaatgt
     9961 gagaggagtt cgtaattatg acagataatt tgcaattagt catcataaca ggtatgagcg
    10021 gtgcagggaa aacagtagct atccaaagtt ttgaagatat gggctatttt tgtatcgata
    10081 atatgccgcc tcgattgatt ccaaaattct gggaattaat caaagaatca ggcaaagtca
    10141 caaaaatggc tttaggagtg gatttacgtt ctcgttcctt ctttgaagaa atccaaaaca
    10201 tgctgatcga aatcgaaaat acgaacttca tcgacacttc tatcttattt ttagatgctt
    10261 cagacgaaga actagtggct cgctacaaag aaacaagacg agcacatcca atggcgatgg
    10321 acggtttgat cactgaaggg atacgtaaag aacgggcgat actagacgac ttgaaagccc
    10381 aagcttccgt gatcattgat acgacgacat tgacaccacg acaattacgt gaaaaaatca
    10441 atgagtcatt caaatcaaag ggagaccaag gatttcggat cgaatttgta tcatttggtt
    10501 ttaaatacgg gttgccgatc gattcggata ttgtgatgga tgtgcgtttt ttgcccaacc
    10561 ctcattatat cccggaacta agaccattga ccggaaaaga tcagccagtc tatgattatg
    10621 tgatgtcctt tgatgaaacg caatcattct accataaatt tttggatctt ctggaaactg
    10681 tattgcctgg atacattaaa gaaggaaaaa gcagcttgac agttgcaatc ggctgtacag
    10741 gcggtcaaca ccgttctgtg gcattgactg aacgtttggc aaatgcttta gggaaaaact
    10801 ataaagtcaa tgtgacacat cgggacaagg ataaaaggaa agagacggtg aaccggtcgt
    10861 gaaaatgaaa acctaccgga taagaaaacc aaagatcgta gtggtcggcg gtggcactgg
    10921 tctgccagtt atcctgaaaa gtttgcgaaa tcaaggagca gatatcacag cagtagtgac
    10981 tgttgcagat gatgggggaa gcagcggtgc aatcagagaa tcgatcgcta tggctcctcc
    11041 tggtgacttg agaaatgtct tagtggcgtt atcggatatg ccacaattct atgaagatat
    11101 tttccaatac cgtttcaaaa aggaagatca gtttttagcc aatcatacga tcggtaatct
    11161 gatcattgcc gcagtttccg agatgcgggg aagcacttat gaggcgattc aattattagc
    11221 aaaaatgatg catgtagatg gacatgttta tccttcttca gaaactcctt tgaccttgca
    11281 tgctgtcttt aaagacggaa cgactgcagt aggagaatct aaaatcgcga tcgaccgtaa
    11341 aaccattgac cgtgtgtatg tctgcaacac aaatgataaa agtgaagcca aagcggctcg
    11401 aaaagtcgtt tcagcaatta tggacgccga catggtcgtt ttgggacccg gaagtttatt
    11461 caccagtatc cttccaaatt tagtgatacc agaaatcggc gaagcaatgc tcaagacaac
    11521 tgctgaaacc gtctatatct gtaatatcat gacccaaaaa ggggaaacgg aacatttcac
    11581 agatgccgat catatacgtg ttttacacaa gcatttgaat cagcctttta tcgatacagt
    11641 attggtcaat acggaaaaag tgcctgaaga ttatatggac ccagagatct atgatgagta
    11701 tctagtacaa gtcgcacacg acttcaacgg attgagaaat gaaggatgtc gagtgatttc
    11761 caccgacttt ttggaattga aagatggtgg tgtcttccac gatggagaca aagtagtaga
    11821 agaattgttc cgtttagttt tcggttcaaa atattaagca atttattgtt ggagaaagga
    11881 ggagaatagg atgtcatttg ctgcagatgt gaagaaagaa ctaactggtt tggaagttca
    11941 tcgagaacat gcgaaagcgg aattagctgc tttgctgcga atgaacggct ctttaggtat
    12001 tgtgaaccag cagtttattt tgaacgtcca aacagaaaat gctgcaattg ccagacgtat
    12061 ctattctctt ttgaaagatc attatcaggt acgaagtgag ctactcgttc gtaaaaaaat
    12121 gaaattaaaa aagaacaatg tctatatcgt ccggttgaag caaggcacaa aagatgtgtt
    12181 gatggatctt gatatcatgg atggtatgat gttcaatgga cacgtttcgg acgagattat
    12241 gggaaattcg caaaaaatgc gttcttactt acgtggtgct tttatggcct caggttcagt
    12301 caataatccg gaaacgagcc gttatcattt ggaaatattt tctatttatg aacagcataa
    12361 ccaagacatt tgcgatatgt taaattatta cggtctacat gctcgaacgc ttgaacggcg
    12421 caatggctat atttcttatt taaaaggagc agaaaaaatt gctgatttct taacattgat
    12481 tggtgcaacc aactcaatgc tccgatttga agatgttcga atcgtacggg atatgagaaa
    12541 ttcggtcaat cgtctagtca attgtgagac agcaaatctg aacaagacga ttgatgcggc
    12601 ttctaaacaa atcgaaaaca tcgaattgat cgaagcaaag gtcggtctac atgcccttcc
    12661 tgaaaaactt caggaaatcg cagaacttcg gcttcaacat ccagaggtca gcttgaaaga
    12721 attaggggaa atgattccct caggtgcgat atcaaaatcg ggaattaatc accgaattcg
    12781 caaaataaat gaatttgcgg aaaaactgcg tgaagaagca gtttaatcca aagaaaaaac
    12841 tgatacatga agcaggagat gcgaatgtat cagttttttt taaaagttat acttgctttt
    12901 cttccgtcaa actataattc catctttact ggagtaacat aatttggaaa ggcttctatt
    12961 gtttttaata caatcgtata aaaagcgggg agacaactga atgaagattt tagttttgta
    13021 cataaatttt tgcttaatat agaagtcctg atatagataa gatcaagtat ttatctataa
    13081 ctggcctgat taattaaaaa actggaagga actgctcgat catccttgag aactgctctt
    13141 cttacttctg gtcaagattc tcgtggttgg tggataagga tggttgataa gttcattgga
    13201 ttagctagtc gaaaacaacc aggtgtaatt aatgtagaag ttttaacaag gggatggagt
    13261 gatttattca gtgataatca taagttcata aatagtaatt gagatgtatg gttggaatac
    13321 gttatacctg aacttgaact accaagattt ggtttagaat ttgagaatcg atgtttataa
    13381 agggagggca aaaatgcgtt tttttacaac ttttactatc attatgattg cagtactttt
    13441 tatttttcta gatatagcaa agcgaaatac agcattttta ttatatcggg ttttacttcg
    13501 agcaggtctc ataacattta tatcgatcgt tggatttttt ctattcacag ttattgtatt
    13561 tatttggaga actccagcac caccattgcc agagattaca tatggagaat ttccttttag
    13621 acttgagtat gaattgaatg aagaattaca tgtgatagaa gacacgttaa tagttgaatt
    13681 tgatggattt ggaatgaacg aaggcatagg gagatatcgt agatggacaa gtcgtttagc
    13741 cagcggagaa gatttagtgt tgctgttaga agttagtgat aataagcaaa tattttattt
    13801 tccaggacct gctaattatt acatgggaga taggttgaat ggttataatc atacttttcc
    13861 tagtgcttct ttcattgaaa gggaaagagg cattactaga agggatattt tacatgacaa
    13921 agaattatta gaacaatttg gtccgcttga tcaaaataca ataaatgaag aagaattgtt
    13981 aaatcaatat aatataaggc tagtgaattg ggaaatatct gaaccgattg ttaacaattt
    14041 tggtgattag aaaacaatta cgataaatag aaacaagcca aatcatcagc aagtaaaagc
    14101 tgatgatttg gcctttctta tacgagtagt aggaattcat agcgaatatt attacgatca
    14161 gtagatattt tgatattaaa ggagtattac gaactagtat gtgagaatgt agaggtgatt
    14221 tgcgaataat gagaaattta tcaaatagaa acaaaatatt aatcattatt gttgttatag
    14281 ctgtgtttca tctgggaacg aatgcggttt taagtagaat aatattgggt cctaagccac
    14341 caagacctgc gattacccga ggggagtttg attttagatt agaatatgaa gtagatgggg
    14401 aaaggattgt gatagaagat acaattgttg cattatttga tggattttct gcagatgcag
    14461 gtagcatggc atggtatcgc acgtggcgtt tgcatttggc gagtgacaag agggcacaac
    14521 aattattgtt acatgagtta gatgatggtc gaaaaatata ttatcatttt ggaggagctg
    14581 gattttatat ggggaagtta gtagcagtgt aactgtaaca aggaaagatt tgccaggtct
    14641 tggcattcga aactccatat tttatatcga gcgtgggaga gatacatcta ttgggaacag
    14701 gaaagggtta actcttgaag agttatataa tgactttgga atccgtcttg ttagttggga
    14761 acacaatcca ccaatagaaa atcgctttga ataatcgaaa tctattaatc catttcatca
    14821 gccagtgaat gttgtaatgg aacaatttgg tttagtgttc aaaaccattg aaatactagg
    14881 ctgtgatcag caaaaatgta tctgaataaa aaagagagca actattaaat gagaggagtt
    14941 aggagttgtt agctttatac gaatcaaagt ggcaaataca gatgcgtatg catttgtgtt
    15001 tgccatcttg cgtttggtag acgagatagg tataagctaa agaaacaact tgtttaaaat
    15061 aaacgaccta gctcaacaaa cgaccatcta atcaactaac agcatacata aaagcaacta
    15121 tttttgaagt agaattgaaa tcgggataac ttaaaaagtg attgggatac tagtgtgaat
    15181 caccgtacat aaatattact cgttaaggga atttgagaaa ttttgtatga aaatttaaga
    15241 attagttagt atgcgcaagt caaaaatatg aataaattat ttggtggaga taatttgctc
    15301 agcttgtcat aagaaatgag ataatctggc agatgcaagc ttttggacac catttattcg
    15361 gaataaggag gctgggacag aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc
    15421 ttccgccgtt tataggtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcctacg
    15481 ctccttattt ttttattttg tttggatcaa ttcttttaat tcctctattg aaatagattc
    15541 atcaaagatt tctttaccca agatgacagt cggaacaaat cgaatatgtg cttgctcagc
    15601 ttcgttgatg acagcttggc tcagctgttc gtctttttgt tctgtcagct ttagtttatc
    15661 tactgcaaaa tccgctactt cttggagaga aagatgtttc cactcgtctt gagtatcgaa
    15721 tatttgtttg atctctttga tcgctttttg accatcgctg attgtgagat accggtgcat
    15781 cacgtttcct cgctgcaagc tttctttttc tttatcgaac aatttgatca ctcgctgcaa
    15841 ttgcccgctt tggactgctt cttctaatag ctcataagat tcttcaaacc attgtcggca
    15901 atacggacaa gataggttga tgaattcgac cattttttta ggagcattcg atgaaccata
    15961 aaggattcct ttttgtgtat ttgttttcgt tgcatcaatc actgatatat ccatgttaaa
    16021 acacctcttt cgcttatagt ttatacatgt ctgacaaaaa aaggcaacga tttagactgt
    16081 ttgtctaaaa attagatgac caattcaaat agacatatgt gaaatatttt tataaataaa
    16141 agaaaaaatg gaatgtttta cgttataatg gaacagataa taagagagaa tatgaacaag
    16201 gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa tacggcagcc gcatatattt
    16261 cttatgcatt taccgagatt gctgcaatat atccaataac acccagttcg accatggctg
    16321 aattagtaga tcaatggtca gcagcaggaa aagaaaatgt attcggtcaa ccagtaaaag
    16381 tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt ccatggatca ttgaaaactg
    16441 gtgcactgac aactacatat accgcttctc aagggctgct tttgatgata ccgaatatgt
    16501 acaagattgc tggggaacta ctgccaagtg ttttccatgt tgctagtcgg gcagtgacga
    16561 caaatgcgtt gaatattttc ggagatcacg gagacgtgat ggctgcaaga caaacaggat
    16621 ttgctatgtt agctgaaagc agtgtacaag aagtgatgga tttagcacct gttgcccatc
    16681 ttgcttctat tgaaggagaa gtaccttttt tgaatttctt tgatggattc cgtacgagtc
    16741 acgagattca aaaaatcgaa gttttggaat atgaagaatt agctcctctg atcaatcagg
    16801 agaaattagc ttcatttcgt aaacgaggga tgaatcccaa tcatccgact gttagtggta
    16861 cgaaccagaa tccagacatc cattttcaac aacgggaaac gatcaacccc tattatgata
    16921 aattacctga aatcgttcaa aaatatatga aagagatcaa ccgattacga ggaacttctt
    16981 atgacttggt gacttattac ggagcagacg atgcggaaga agtcattgtt tctatgggat
    17041 cagttgcaca gacgattgaa caaacgattg attacttgaa tgcaaacgga cggaaaacag
    17101 gttttttgaa tatccatctt tatcgtcctt ttccagtgga aacattcgtg caaaagttgc
    17161 ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa agaaccaggg gcaggtggag
    17221 aacctctctt attagacgtt caaagtgcgt tatatgattc tgaacttcgc ccggctgtta
    17281 ttggtggtcg ctacggattg gggtctaaag atgtgactcc cgaccaaatc tcagcagttt
    17341 ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg gtttaccata gggatcgtgg
    17401 atgatgtgac ttatcaatct ttggaaccaa aggaaagtct ggatttgaca gaaccgcaaa
    17461 cattccaggc caagttttgg ggatttggat ctgacggaac agttggagca aataaatcag
    17521 ctatcaagat tattggagat cacaccgata aatatgcgca aggctatttt tattatgatt
    17581 cgaaaaaatc tggcggtctg actgtctctc atcttcgttt tggagatacg ccgatacgtt
    17641 cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca cacaccagct tacctgcata
    17701 gctatgactt ggtcaaagga ttgaagccag gtggcatttt tttattgaat actctttgga
    17761 gcgacgaaca attagaaacg catctgccct taaaattaaa acgttacttg gctgaaaata
    17821 acattagatt ctatacgatc aatgccatga gattggccca agaagtcggt ttgggacgcc
    17881 ggatcaatac ggcaatggaa acggcttttt ttaaattagc tgacatcatt ccttttgatg
    17941 aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta tggacataaa tcaatggcta
    18001 tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga actgcttcat caagtcgcga
    18061 tcccagacca ttggagaacg ttggagatgc ctgaaaaaat caataaaagg aacactggtt
    18121 atatacaaga aatacttgaa ccagtgaatg cacaggaagg aaatcaactc tctgttggta
    18181 cacttgtagc aaatggaatg acaactggcg aaatgccttt aggaacggct tctatggaaa
    18241 agcggggcgt cgctttagaa gtacctgagt ggattagtga tcgctgtacg atgtgtaatg
    18301 aatgtgcctt tgtttgtcct catgctgcca tccgtccatt tttagcagat gaagaggaaa
    18361 tggaagaagc gccagaagga tttatcgtga gagaaatgcg aggcgcagac ggcgtaaaat
    18421 accgtatcca agtttcagtg gaagactgta caggttgtgg tctgtgtgta gaggcttgtc
    18481 ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga acagaaagaa caagctgtga
    18541 attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc agtcaaagga aagaatactg
    18601 tcttgtctac acaattcaat caaccgcttc ttgaattctc gggcgcttgc tcaggctgtg
    18661 gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg tgatcgaatg atgatcgcga
    18721 atgcgacagg ctgttcttct atttggggcg gtgcttctcc tgcttctcct tatacgacca
    18781 atgaatgtgg tcaagggcct gcatggtcaa attctttatt ggaagataat gctgaatttg
    18841 gttatggtat gcttctagcg gcacagactc gtcgcgaggc tttggcaagt tcaatcaaag
    18901 aagtattgcc aaaagtatca gaagaactac gagaattgat ggaagactgg ttggtacaca
    18961 tgtttgagag cgaaggaacg cagcaaagag cagcaaaact gcaagctgcg atcgaaagcg
    19021 aacgatcaat tgtccctgaa ctggaggata tatacaagag aaaggatcta ttcgtaaaaa
    19081 acagccaatg gatgattggt ggagacggat gggcttacga cattggtttt ggcgggatcg
    19141 atcatgtact tgccagcgga gcagacgtga atattcttgt tttggataac gaagtctatt
    19201 ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc ggcaatcgct aaattttcgg
    19261 ctagtggaaa gtatgcttca aagaaagatc taggaatgat ggcgatgaca tatggaaatg
    19321 tttatgtggc gcaaattgct tcaggtgcca atcaaatgca gacgataaag gcttttgaag
    19381 aagcagaaaa gtatcctgga ccttctttaa tcattgccta tacgccttgt atcacacatg
    19441 gtttgcttgg tggaatgaaa gaatcgctta aagaagcaaa agaagctgtt tcttctggct
    19501 attggtcatt atatcgatat aaccctgcaa tggtcgaaaa aaacaagaac ccaatgactt
    19561 tggactacaa aaaacctgat ttctctgcta tgcctgattt catgaggaaa caagttcgct
    19621 tttcctcgct agagaacgca aatccaaatc ttgcaaaagc tttatttgaa aagactatcc
    19681 aagacgctaa aacacgtttt tacaactatg ctgcactgac gggagaatta gaaaaaatca
    19741 agaaacgact tgaaccagag gaatctatcg atgtacccgt taagactaga aaagaaaaaa
    19801 cgacggacct tgaagcagaa gaaagaagag ctgccagagc agaacgacgt aaaaaaatgg
    19861 aacaagctac aaaagaaaaa aaggaaaaat aacagaaaaa atataatgaa taattctctg
    19921 aagtttaaaa atatttattg cagagaaaaa taaaacgcat acttgcaatt ttcctgaaaa
    19981 ttatgcgttt ttctcaagaa aaatacgagc agatcagcct ctaaaattaa aacatatgat
    20041 atactagaga ggaaatctca aaagagaaga gaggcttttt atgtcatttc aaatcgggga
    20101 actttttaat ttggactatt ggcagcaaat gttttccggt gacttgttct ctctgaattt
    20161 tttggtgaac atacttgata ttctggttgt ctggtatctt gtatacaaac tgatccaact
    20221 gctgcgaggc acaaaagcga ttcagcttct gaaaggtgta gctattttta ttgttatccg
    20281 aatcgtagcg gaaatcattg gtctgcacac cttgtcttgg ctgatgaacc aagtcattac
    20341 gtacggggtg atagctgcta tcgtgatttt tcagccggaa atccggagag gactggaaca
    20401 tcttggacgc agttcgcttt tcaaacaatc aaaaagcgaa cagcaggaag atgagaagat
    20461 gatcttgtct tttgacaaag ccatccaata tatgtccaag cgaaaaatcg gtgcgctaat
    20521 tactatcgaa cggcatactg gattagatga gtatattgag acaggaattg cattagatgc
    20581 agatatcact ggagagctgc tgatcaatat ctttattcct aatacaccgc tgcatgacgg
    20641 tgcagttatt gtaaaagaag gaaagatcgc agtagcaagc gcctatcttc ctctatcaga
    20701 aagtatgttg attccaaaag aattcgggac tcgacaccgg gcagctgtcg gtataagcga
    20761 agtcagcgac gcgattacca tcgttgtctc tgaagaaact ggggatgtca gcattacatt
    20821 agataatgaa ctgatggctg gcttatccca gcaagaatat ttggcaattt tgcgtagaga
    20881 actgattgct gaagagaaaa caaaaaacaa gaaaaacgtg attcaatctt tcctagacgg
    20941 tatgactaag ggaggggatc gcaaatgatc tcaaaagaga aacggacaag cattctttac
    21001 agttttattg cattgctgtt cagtattgcg ctgtacttca atgcaaatgg ccagtccgtt
    21061 ccaaatacgc tttctggaaa tgaagcgtat aatcaaacag ttactggggt gccaatcaag
    21121 atttcttatg attcaaagcg ctactatatc catggatatg aaaatgcagt gactgtcaaa
    21181 ctttccagtg caaaccgagt tcagttgaat gcagaggcaa acgaagatac acggatgttc
    21241 cgcgtcacag cagatatcac taagctgggc gaaggaacac atgaggttcc gctaaaaatt
    21301 caaaatttga gcagtgctgt cacagcaaag atcgaaccat cgactattac agtaacggtt
    21361 gagaaaaagg taataaaaga tttcgatgtg gaaacgttga ttccttcaac gttcacacca
    21421 tctggatatg aacttgatga tacttccgtt agtcctaaaa aagtctctat cactacaggt
    21481 gacaaaacat tggcagaaat caaacgagta gtagctgatg tggacgcttc gatggtcaca
    21541 gatgatggga ttaatagtga ggtcccaata caggcgctga atgcagcagg ggaagtatta
    21601 agtatcgtct ctgatccagt gcaagtaaca gtgagagctg attcgatcaa gccaagcaag
    21661 tcggtacgac tttatgggat acagcaagga acaccagctg ccggggtaga aagctatgat
    21721 ttcagttttt caaaactgga agcagaagta agcggctcta gcgatttgct tgcatcgatc
    21781 ggtgacagta ttgctgtacc gatcaatgtc tcaggtatca atcatcgaac aactcgtaaa
    21841 attgaaatac cagtagaaga ggggttaagt atcaagcccg aatcagttag tgtggagatt
    21901 acacccgtgt tgcaaacgtc aagcagtagc tctgcttcga cgagcagtcg gacacaagaa
    21961 aatacaagta cgagtagaag tatttcatct gctgattcag gaacggaacc atcgacaaca
    22021 accatttctt ccagttcgga atctgggaca agcagctcga ccacaagtac agaagaaaca
    22081 tcagaaacaa gcgaatcaac acaaatgagc agaacaaatt cctcagaaaa ttaagttaaa
    22141 ggagtaattt tacaactatg ggtaaatatt ttggaacaga cggcgtacgc ggtgaagcca
    22201 acaaggaact tacgccagaa cttgcattta agctgggacg gtgcggaggt tatgtcctaa
    22261 gccagcatga agaagaagga aaacgcccac gcgtgttagt agggcgggat acaagaatct
    22321 caggtcaaat gttagaagaa gcgctgattt caggactttt atcggtgggt atcgaagtgt
    22381 tccagctagg tgtgatctct acaccaggtg ttgcttatct gacacgtttg caaaaagcaa
    22441 gcgcaggggt catgatttct gcttctcata atccagcaca agataatgga atcaaatttt
    22501 tcggtgcaga tggttttaaa ctagtagatg accaagagct agaaattgaa gcattattag
    22561 atgctgaagt ggatgaattg cctcgtccat cagctgaagg attgggaact gtagaagaat
    22621 ttccagaagg cttattgaaa tattctcaat tccttgtaca aacgatcaac ggtgaccttt
    22681 ccggtttgac catttgtgtc gatgcagcaa acggagcaac ttcaacaagt gttaatcgtt
    22741 tgtttgccga tcttgaaaca gatttttata ccatgggcac aaatccagac gggttaaata
    22801 tcaatgatgg cgtaggctct acccatccag aacgtttagc tgagatggtc gttgaaaaag
    22861 gagcagatgc tggactagct ttcgatggcg atggcgatcg tattattgcg gtggatgaac
    22921 ttggaaacat tgtcgacggt gataaaatca tgtttatctg tgctagatac ttggcagaaa
    22981 aaaatcgatt gaaaaaagat acgatcgtta cgactgtcat gagtaactta ggattccaca
    23041 aagcggtgga aggaatcggt ttgaaagatg tcgttacaca agttggtgat cgttacgttg
    23101 tagaagaaat gcgcaaaaac gattataact ttggcggcga gcagtctggg cacatgattt
    23161 tcttagattt taacacaact ggagatggga tgctttcagg aattcagtta ctgaatatca
    23221 tgaaacagac aggtaagaaa ttatcagagc ttgcagcaga ggtaacaatc tatccgcaaa
    23281 aattagtgaa tatccgagta agtgataaaa acggtgcgat ggatgttccg gcaatcaaaa
    23341 aagtgattga agaggcagaa gctgaaatgg gatcagaagg aagaatccta gttcgtcctt
    23401 ccggtacaga accattactt cgagtgatgg cagaagcgcc tactgatgag aaagttaatt
    23461 actatgtcga taaaatcgcg gcagtagtca aagaagaaat cggactggat taagagatat
    23521 tttcataaaa aataaaagaa caaggaccag tgtcagatgt ctgacactgg tccttgttct
    23581 tttattattc tactgtcact gatttcgcta aattacgtgg tttgtcaacg tcgaatcccc
    23641 gtaatagcgt tgcgtaatat gcaagtaatt gagtcggtac gactgtggtt agagtagtta
    23701 gataagggtg gacagttggt agcaccagct ggtcactttc cttttctaat ccctctgaga
    23761 caataactag tgttttagcg ccacggcttt cgacttcttt caggtttcct cgagtatgcg
    23821 caccagtcac ttcttcactg atgattccaa tcactggtgt tccttcttcg atcaaagcaa
    23881 tcgttccatg tttcaattcg ccagcggcaa aaccttctgc ctggatataa ctgatttctt
    23941 tcaatttcaa agcagcttca agagaaacgt tgtagtcagc gccacgtcca atgtaaaagg
    24001 catttcttgt atcgctgaga tatacttctg ccaactcttc taaataatct ttttgggcga
    24061 tgaccgattc catcgcatta gcaactaaac tcaattcatg agtgatatcg aatacaagag
    24121 aagtttcaaa ttctttttta tcaccgatag ctttggctaa taaagttagt acagctattt
    24181 gtgcagtata agctttcgtt gatgctactg cgatttcggg tccagcatgc aatagcagtg
    24241 taaaggatgc ttcccgcgaa agagtagaac cagcaacatt tgtaatcgta agtgaagggt
    24301 agccgagacg gttgattttt accagaactt ggcggctatc tgctgtttcg ccactttgac
    24361 tcaagaaaat aaagaatggt ttttttgaaa gaagaggtgt gttatagcca aattcactgg
    24421 ctaaatgaac ctcaacaggg atctgtgtca atttttccag agtctgttta cctgctaacc
    24481 ccgcatggta actagttccg caagcaacga tatagattcg atcgctatca gacaatgtat
    24541 cgaccagctt ttgatcaaga gagacacgat tgtcttctgt tacgtatttt tgagcgatac
    24601 gacgcatgat aataggctgt tcgtcgattt ctttcagcat ataaaaagga taggtacctt
    24661 tctcgatatc tgaagaatct acttgtgctt cgaatgaaga acgttctacg atgtcacctg
    24721 caaaattttg aataataact ttttctgatg tgacggtaac catctcttca tcttcaatct
    24781 caacgaattc cttcgtatga gcaagcatag ccattgcatc gctggcaatc acattgaatc
    24841 catcgccgag tccgattaat agtggacttt tatttttagc gacgtaaata acatcaggtg
    24901 ctgtccgatc aatcaaagcg aaagcgtaag aacctttgat gacccgcaaa gcttttaaaa
    24961 acgcctcttg cgttgtagta tccttcgcga aagtttcaat tagatgagcg ataatctcag
    25021 tatcggtctc accaataaag tggtcatttg ctaaataagc aattttaagt tcttcaaagt
    25081 tttcgattac gccattgtga acgagcacga agcgtccact ttgggaagta tgtggatggg
    25141 cgttttcttc agaaggttgc ccgtgagttg cccagcgtgt atgaccaata cctatatttc
    25201 ctgtagtatc aatatttatt ttgtttttca aattttgaat tctgccttgt gctttgacca
    25261 gataatcagt tgtgccatct gagacaaaga taccagctga atcataacca cggtattcta
    25321 atttttctag accgtcaatc aatcctggtg ttacattttt taatccaatc attccaacaa
    25381 ttccgcacat aaaaaacatc ctactttcta taatgaattc gtatgaatgc ttccagctgc
    25441 aggaaaatcc ttttgtttta tctttgcaga tattttttgt ggattttctg tagagtgcag
    25501 tccctagggc catccgccga atctttcgat aaaccctctc ctcgtcatct gtttacacag
    25561 acctggcgct atcctaaaag gtataactat cctcctcttt tgttttttat ctggaaacat
    25621 ttaaaatcat aaccttttgc gaatgttctt gtcaacaaaa aaagaataaa aaataaattg
    25681 gtatatatca aatgctttgt caaatgctct ttttatgtca aaaaataatg ggtttttatc
    25741 tatcatcttt ttcaggagtt cggtacaata gaaagtaatc ggatagagga ggagtaaaaa
    25801 tggattcaaa aattgagatc atgacactgg gaatgttaaa aaaacaattg tccaaattcg
    25861 aagcatcagc tggtgtatca gatgatacga aaatattttt agatactggc tgggatagca
    25921 tccaagaaat cgcacctgat gccttggaag ttgctgaagc tcgtgagttt acggtagaag
    25981 atgaattgac aaaagagtcg ttttccgggt atgcgcggga agaaaaagca gagcggttcg
    26041 acacttctga gcagtcagaa actgtgatcg tcataaaaaa cttgtattag aaaggagaaa
    26101 gaatgaataa gagacgttgg gcagcagttc tgattgctgc aggattattt gtggtttcgt
    26161 tagtttcagc tagtttgact aagcctcagg aaaacgatca aattgaggga ttgaacagtt
    26221 ggttatatgg ggatgaagaa ctagcttcaa atgtgttgga agagggagac agtacgcaaa
    26281 aaatcgtaaa attatcagta gatggaacga ttgctgcggg agggtcttca ggtttatttg
    26341 cttctgaagg ttacaatcat aaaaatttta tggaacaact gaaggcgatt gaagaagatc
    26401 caacagtcaa aggtatcttt ttagaagtaa attctcctgg tggcggtgtc tacgaaagtg
    26461 cggaaatcgc taagaaattg gatacgattc gtaaagaaca tgatatccca atgtatgtca
    26521 gcatgaaaaa catggcagct agcggtggct actatatttc tgctcaagca gataaaatct
    26581 ttgcaacaga agaaacagtg accggctcaa ttggtgtgat catgtcagga ttgaattatt
    26641 caggacttct agaaaaactg ggtgtggaag atacaacagt taaaagcgga gcgttgaaag
    26701 atatgggttc aggaacgcga ccagagacaa aagaagaaga agcggtgctt caagcatata
    26761 tcgacagcgc atatagtcgt tttgtagcaa tcgtcagcga gggacggaat aaatctgaag
    26821 aagaagtcaa aaaaattgct gatggaagga tttatgatgg tgtccaagca aaagaagtag
    26881 gtttagtcga tgaattggga tttcctgaag atgctttaaa agctatgcga aaggaacaac
    26941 agcttgaaga tgcggaacta gtagaatatt cttccacgcc atctgggttt gctagtacat
    27001 ggttaggaag taaactagca gaattccagg gattgaagcc ttctgagact agtcagatct
    27061 tatccgttct tgagaatcta gggacagcgg aatcaccaaa agcaatgtat tattacggag
    27121 gtgagtaagc atgtcagagc tttcaaatga aaataaggaa caagaaaaag ataagcaaca
    27181 gcagcctgct gtaggacctg actccaactt ttcccaaaag gtactgagct cttttgaaag
    27241 agagcctttg acgcccgaag aaatcaaaaa gaaacaacgt aaatggaaaa agtatacgga
    27301 cacaagaaat gaaccgccaa aagcaaatga ttttcctaag tatttctatg caggtttttt
    27361 tatccgaatg tttgctttct tggtcgatct tttgtgtatc ggagctatta cgcgtttaac
    27421 acttggtatt gccaaaaatc taggttggct taatttttca gattcttatc tgagtatcta
    27481 cggattcaca gccttactta tttatctagc gtattttatt ttattgacga aattcaatca
    27541 tggtcaaacg attgggaaaa tgatctttgg tattcgagtc atcagctttg atgaagaaga
    27601 acttagctgg tcaaccgttt tagttagaga aggtgcttgc cggttcattc taaaatttcc
    27661 tttgctgatg ttagggtatc ttccagcaat ctttaatcag aaaaagcaac atctaggcga
    27721 ttttttcact aatacaagtg ttgttacgat caatctgatc aaagcattca accaggaagt
    27781 caacagctga tgagcaataa aaagcagtaa cagttgaaag caccttctaa aagtcagatc
    27841 taaaatctag cttttagagg gtgcttcagt tatttttaat tgtcatgctt tttttgtttt
    27901 gctactattg aacatgagct gaacatgagc aagaggagga cactgcgtga agccgattga
    27961 ttttccagaa aaatatgaga atttaatgcg agtagcacaa caagcactag caaaccagca
    28021 atatcagcag gcaaaagaat tatttcaacg agcttacgag ttgaaagaaa gctttgaggc
    28081 taatagcctg cttgtttttt gtctttacga gttggacgag aaaaaagaag cattgaagca
    28141 agcactcttg catgaaaaac agtatcttca aaatgaggag tttgctgaat tttattttga
    28201 cttgttaatc agtacacaag attttttata tgctagaaaa ttgatagcaa gtacagattt
    28261 ttatgaagct ttcgagcagc gggtcattga aaaaatccaa tttgcagaag aattatccgg
    28321 acaaatggag aggcagaaga tcaaggcact ccacaataaa tcggaggaac ttccttcgtt
    28381 ggaacctgca cgtcagcttg cgtctattga tgagatcgaa cagcttcctt atcatgagtt
    28441 tatccaaaca gcgagtaaac ttattgtatc gccagaggtc catattttag caagggcaaa
    28501 attactggag acacttaggc aattgaacga atgccatcct gttttctatt tgacgattga
    28561 agaaaagtta gtcaaagtca taccaaaaga tctgccaaag ccgcagcagc aggcaagtta
    28621 ccagcaatta tgtgtatttt cagatcgtta cggtaatgaa gatgcgctgc ttagttccgt
    28681 gttaaatgaa gaattcacgt tgcaatctgc aattgtttat cctgtctatg atacatacat
    28741 cgaagacccg aagcgctggt ttcaactgac tgtagaggcg tgtacaggaa aaacgatgta
    28801 tgaaggtaca gaagatgaaa aacaagattt tttaaaaaaa agagaaaaaa ttttgcagca
    28861 aatgatcttc ttccattaga ttttatcagt gaaaaagata aaaatgttat gaaaaacaag
    28921 gattctatcc attcaatatg tttacaattt gaaagggtta gtgtactatt taaaggtatg
    28981 catgaaacgt ttttgatgta taaaatgaat ttctaaactc ggagggaatt aaatgactgc
    29041 aacatttgaa aaaaaaggca cgaatgatgg tgttttaaaa tttacgattg cgcaagacga
    29101 aatccaaaaa ggattaacaa ctgcgtttaa taaagtgaaa aaaagcttga acgtacctgg
    29161 attccgtaaa gggaaagttt ctcgtcaagt attcaaccgt atgtacggtg aagaagcttt
    29221 atatgaagat gcattgaatg ctgtattacc agaagcttat gaagctgctg tcaaagaagc
    29281 tggcatcgat cctgtttctc aaccaaaaat cgatgttgaa agcatggaaa aaggtcaaga
    29341 ctgggtaatc actgctgaag taacagtgaa accagaagta aaattaggac aatataaaga
    29401 tctagttgtt gaaaaacaag accgtgaagt aacagatgct gacgtggatg cacgtatcaa
    29461 acgtgaacaa gaagcacaag ctgaattagt tatcaaagaa gatgctgcag ctgaaaatgg
    29521 cgatacagta gtaatcgact tcgaaggctt tgtagatggc gaagcgtttg atggcggaaa
    29581 aggcgaaaat tactcacttg aattaggttc aggctcattc attccaggtt ttgaagatca
    29641 attagttggt cataaatcag gtgaaagtct agatgtaaat gtaactttcc cagaagatta
    29701 tcaagcagaa gatcttgctg gaaaagaagc agtcttcaaa gtgacaatcc atgaagtaaa
    29761 agcaaaagaa gtgcctgaat tagatgacga attcgcaaaa gatgttgatg acgaagtatc
    29821 aacattagac gaattgaaag aaaaatatcg caaagaatta gtagaaacaa aagaaaaagc
    29881 agcggacgat gcaaaagatg aagcagcgat ccgtatggct gttgaaaacg cagaaatcgt
    29941 tgaattgcca cacgtgatgg ttcatgatga agtacatcgt tcaatggacg aattcttgaa
    30001 caacatgcaa cgtcaaggta tctctcctga aatgtactat caattaactg gttctacaga
    30061 agaagacttg cataaacaat tcgaaggtga agcagaaatg cgcacaagaa caaatcttgt
    30121 gatcgaagca attgcagctg ctgaaaatct tcaagcaaca gaagaagaaa tcaatgcaga
    30181 aatccaagaa ttatcagaaa catacaacat gccaatcgaa caaatcaaac gcgtgttgac
    30241 agaagatatg ctggaacatg atgtgacaat gaaaaaagca atcgaattga tcacaggtac
    30301 agcgacagaa gcgaaagaat agaaatcaag taataaatca tttctgaaga agctacttct
    30361 agaaaattgt ttattcgaaa taagggactg tgacacgatt ttgtcacagt tccttatttt
    30421 ttgttaagag taaaaaatgc aaaggtatag gtcagttgat cctttgttaa acactttttc
    30481 tttttactaa actatgttat gatagtaaaa gactgtaaag tctttgatag tttcttgcat
    30541 actttttgca agttaagggg tgaagaccat gtatgacaat ccaagtagca atgagaccgt
    30601 acgctgttct ttttgcggga aaacccaaga agaagtgaaa aagatcgtag ctggtccagg
    30661 ggtctacatc tgtaatgaat gtatcgacct atgtaaagaa atcattgatg aagaatttta
    30721 tgaagaagct gtacgcgaat tcactgaagt ccctaaacca ctagaaattc ttgaagtatt
    30781 gaataactac gtgattggac aagatcgcgc aaaacgctcg ttagctgttg ccgtatacaa
    30841 tcactataaa cgtgtcaatc agcaagcaca agaaaacaca gatgatgttg aactgcaaaa
    30901 aagcaatatc tgcttgatcg gacctacagg gtctggtaaa acatttttag cacaaacatt
    30961 agcaaaaaca ttgaatgtac cttttgcaat cgcagatgcg acaagtttga cagaagctgg
    31021 ttatgtgggg gaagatgtag aaaacatttt gcttaaattg cttcaatctg ctgattacaa
    31081 tgtggaacgt gcagaaaaag ggatcatcta tattgatgag atcgataaaa ttgcgcgtaa
    31141 aagcgaaaat gtatctatta ctcgtgacgt ttccggcgaa ggtgtccaac aagccttgct
    31201 gaaaattcta gaaggaactg ttgcaagcgt gccacctcag ggaggacgta aacatccaca
    31261 tcaagaattt attcagattg atacgacgaa catattgttc atcgtaggtg gtgcatttga
    31321 tggcatcgaa acgattgtga agaatcgtat gggcgaaaag acgattggat ttggtacaca
    31381 aaaccaaaaa ctttctgaag acgaaagtgt catgcagcat atcattcctg aagatttact
    31441 aaaattcgga ttgatccctg agtttatcgg acgattacct gttatggcag ctttagagaa
    31501 attgacaaca gatgatctgg tacggatctt gactgagccg aaaaatgcgc tagtcaaaca
    31561 atatcaaaaa cttctttctc tagataatac cgaacttgaa ttcgaacctg aagcgttgag
    31621 agccattgcg aaaaaagcaa tcgaacgcaa taccggtgct cgaggacttc gttccatcat
    31681 tgaagaaatc atgatggatg tgatgtttga tatcccttca aatgaaaaca tcgaaaaagt
    31741 gattatcaca aaagaagcag ctgaattgtc tggtgagcca acagtaatct ataaagattc
    31801 agagaaaaaa gcgggttgat gaaaagaggc tgccgtaaaa gtcattttgg cttttgcgac
    31861 agccgcttgc tttattcatc accttgttaa ggagagagac catgaaagtc catcaagctg
    31921 aaattatcat aagtgcagtg gcaccaaaac aatatccaga tacacaattg ccagaaatag
    31981 ctctggcagg acgatcaaat gtagggaaat cttcttttat caacacattg attgatcgaa
    32041 agaatttagc ccgtacttcc ggtaagccag gaaagaccca gacattgaat ttttatttga
    32101 tcgagaacgc attacatttt gtcgatgttc cgggatatgg gtatgcaaaa gtatccaaaa
    32161 ctgagcgagc aaaatgggga aagatgatcg agacatattt aacgactaga gaccagctac
    32221 gtgcggtcgt ttctttagta gatttgcgtc ataagccttc tcaagaagat atccagatgt
    32281 atcaattttt gaagtattat gatatccctg taattgttgt tgccactaag gcagataaga
    32341 tccctcgtgg taaatggaac aagcatgaga gcgaaatcaa aaaagcgttg gattttgatc
    32401 gcaatgacac gtttattctc ttttcttccg ttacaaaaca gggaaaagaa gaagcgtggg
    32461 cagcaatcga acaagcaatt gaatcataaa aagaataaaa aagacttacc agtcaaactc
    32521 tttcataaaa gtttggctgg taagtctttt tttgagctta acttactttt gtttcttgtc
    32581 ttttttcttt tcattttgtg gctgtggttt tagaaatcga tcgatctctt cttttggtgc
    32641 aactgttttt tccggatcga cagcaaatga ttcgaaaagt ttatctaaat tatctgtacg
    32701 ttcaaatttc aaccccattg taaacacctc tttgtttctt tttacttagt ctatcatgta
    32761 atcgtccaaa ttggtttcta agacataaag ttttagtttt tctttatcgt tggttataat
    32821 taaattataa aaagatttta aagaggtgag aaaatgattg gcgaagataa attattgatg
    32881 actatccagc tcacagactt aaatgaagaa gaaaaaaaac aggaaataag cgattgggct
    32941 attttgacac gtctgttgat ccagccaaca tttatccgct ctttcacaca acaagcggat
    33001 ccgtatgatc ttcgaaagct tcaagaaaaa ataatgctag catcggtaag agacgaaagt
    33061 tggttagttg taggaaatga cgagaacgaa tgtagttttc gcttggaaga taatcaatta
    33121 ttgataaaaa atgtattgtc tatctctgtt ttcaaagaaa agcaattttt gattcgagac
    33181 ttcattcaga aaaaaatgat tgctcatggg gtgttcgcct atatgcgagc ttatagcgag
    33241 tttatctatc acaatacaaa acagatctct caacggctat tgtttgagaa aaaggatgaa
    33301 atcgaacatt tacctaaaat gaaacaacaa aacggggaag tagtagtcga ttgcaaccaa
    33361 tttccaggat acgatctttt ttaccaaggt ttatgtttca cttcgtgttg ggaaatgtat
    33421 tatagtcgct attatcatca aatcatccct aaaccgattt ttctcgacat ccaacaagta
    33481 gaaaaagtga aggaattaga aaacgaagtc atatgtatcc agctttatcg agaccccttt
    33541 aactggcaaa agccaaacaa tcaaatgttt caatcctatt ttagggacca gcttgggttt
    33601 gatcatcttg cttgggacaa tggtgtcggt ttattgaaac cgccattcgt tgaatacatt
    33661 tacactgacc atacgatcca atctgtccag tatcagaatg cacaaatgca acctgttcct
    33721 aaaaagaatg cttcattttt tgtaacaaaa agttatgata ttcaacaagg agattataaa
    33781 gaaaggcgtg ttcgcggaac actaaatgct caggcgtatt ttccttgggt tgacgagaat
    33841 cgctcgcgca tgatgtgcta taaagttatc gatccgactg ttgctttgga taatggaatc
    33901 gaagcttatt gttattatat tagggaatat ctagaagtag aggtaacaga tgaaaaatat
    33961 caagagtatc ttttatcctt aagaatctat gttccttcag aatccctgtc agaattaccg
    34021 ttaaaggaaa tcaaacatag actctctgat gttaccttca aacgaataaa gaaaaagcgg
    34081 cgaagtatcc aattcgatgt caagaaaggt gagcagcatc tgcgtgtgca atttttagat
    34141 taccgagaat tagaacaatt gattacttta caaaaaatat gaggtgaaga aaatggataa
    34201 acaactctat tatcgaataa tggttccggc atcattatta tccgtctgtc tcttttttgt
    34261 tcctaatttt cctattcgat cagtcatcgg ttttttggtc atgtttggcg ggatgggact
    34321 ttattattat cttcttcata aggaaagaaa acctgcttct gtttatatga aaaaaacaag
    34381 aagcgaaaat aaaaaacgat aaaacggctt gatgatagac aaaaaaata
//
LOCUS       DAZAJZ010000020        34420 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000020 DAZAJZ010000000
VERSION     DAZAJZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.020"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            348..1037
                     /locus_tag="ACG86D_000952"
     CDS             348..1037
                     /locus_tag="ACG86D_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3140119.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1024..2232
                     /locus_tag="ACG86D_000953"
     CDS             1024..2232
                     /locus_tag="ACG86D_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN3140120.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2526..3797
                     /gene="serS"
                     /locus_tag="ACG86D_000954"
     CDS             2526..3797
                     /gene="serS"
                     /locus_tag="ACG86D_000954"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN3140121.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4346..5830)
                     /gene="guaB"
                     /locus_tag="ACG86D_000955"
     CDS             complement(4346..5830)
                     /gene="guaB"
                     /locus_tag="ACG86D_000955"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN3140122.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6335..7030)
                     /locus_tag="ACG86D_000956"
     CDS             complement(6335..7030)
                     /locus_tag="ACG86D_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN3140123.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7120..8220)
                     /gene="ychF"
                     /locus_tag="ACG86D_000957"
     CDS             complement(7120..8220)
                     /gene="ychF"
                     /locus_tag="ACG86D_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN3140124.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8229..8420)
                     /locus_tag="ACG86D_000958"
     CDS             complement(8229..8420)
                     /locus_tag="ACG86D_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN3140125.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8441..9331)
                     /locus_tag="ACG86D_000959"
     CDS             complement(8441..9331)
                     /locus_tag="ACG86D_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN3140126.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9318..10085)
                     /locus_tag="ACG86D_000960"
     CDS             complement(9318..10085)
                     /locus_tag="ACG86D_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN3140127.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10097..10810)
                     /gene="rsmG"
                     /locus_tag="ACG86D_000961"
     CDS             complement(10097..10810)
                     /gene="rsmG"
                     /locus_tag="ACG86D_000961"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN3140128.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11042..12943)
                     /gene="mnmG"
                     /locus_tag="ACG86D_000962"
     CDS             complement(11042..12943)
                     /gene="mnmG"
                     /locus_tag="ACG86D_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN3140129.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12960..14357)
                     /gene="mnmE"
                     /locus_tag="ACG86D_000963"
     CDS             complement(12960..14357)
                     /gene="mnmE"
                     /locus_tag="ACG86D_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN3140130.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14656..15039
                     /locus_tag="ACG86D_000964"
     CDS             14656..15039
                     /locus_tag="ACG86D_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140131.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15207..15965)
                     /gene="jag"
                     /locus_tag="ACG86D_000965"
     CDS             complement(15207..15965)
                     /gene="jag"
                     /locus_tag="ACG86D_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN3140132.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15983..16801)
                     /locus_tag="ACG86D_000966"
     CDS             complement(15983..16801)
                     /locus_tag="ACG86D_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN3140133.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16798..17154)
                     /gene="rnpA"
                     /locus_tag="ACG86D_000967"
     CDS             complement(16798..17154)
                     /gene="rnpA"
                     /locus_tag="ACG86D_000967"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN3140134.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17272..17406)
                     /gene="rpmH"
                     /locus_tag="ACG86D_000968"
     CDS             complement(17272..17406)
                     /gene="rpmH"
                     /locus_tag="ACG86D_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN3140135.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17929..19263
                     /gene="dnaA"
                     /locus_tag="ACG86D_000969"
     CDS             17929..19263
                     /gene="dnaA"
                     /locus_tag="ACG86D_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN3140136.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19461..20591
                     /gene="dnaN"
                     /locus_tag="ACG86D_000970"
     CDS             19461..20591
                     /gene="dnaN"
                     /locus_tag="ACG86D_000970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN3140137.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20820..21062
                     /gene="yaaA"
                     /locus_tag="ACG86D_000971"
     CDS             20820..21062
                     /gene="yaaA"
                     /locus_tag="ACG86D_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN3140138.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21049..22173
                     /gene="recF"
                     /locus_tag="ACG86D_000972"
     CDS             21049..22173
                     /gene="recF"
                     /locus_tag="ACG86D_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN3140139.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22170..24116
                     /gene="gyrB"
                     /locus_tag="ACG86D_000973"
     CDS             22170..24116
                     /gene="gyrB"
                     /locus_tag="ACG86D_000973"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN3140140.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24136..26607
                     /gene="gyrA"
                     /locus_tag="ACG86D_000974"
     CDS             24136..26607
                     /gene="gyrA"
                     /locus_tag="ACG86D_000974"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN3140141.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26843..27142
                     /gene="rpsF"
                     /locus_tag="ACG86D_000975"
     CDS             26843..27142
                     /gene="rpsF"
                     /locus_tag="ACG86D_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN3140142.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27192..27704
                     /gene="ssb"
                     /locus_tag="ACG86D_000976"
     CDS             27192..27704
                     /gene="ssb"
                     /locus_tag="ACG86D_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN3140143.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27730..27966
                     /gene="rpsR"
                     /locus_tag="ACG86D_000977"
     CDS             27730..27966
                     /gene="rpsR"
                     /locus_tag="ACG86D_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN3140144.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28109..30082
                     /locus_tag="ACG86D_000978"
     CDS             28109..30082
                     /locus_tag="ACG86D_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN3140145.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30088..30540
                     /gene="rplI"
                     /locus_tag="ACG86D_000979"
     CDS             30088..30540
                     /gene="rplI"
                     /locus_tag="ACG86D_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN3140146.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30814..32181
                     /gene="dnaB"
                     /locus_tag="ACG86D_000980"
     CDS             30814..32181
                     /gene="dnaB"
                     /locus_tag="ACG86D_000980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN3140147.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32961..34253
                     /locus_tag="ACG86D_000981"
     CDS             32961..34253
                     /locus_tag="ACG86D_000981"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN3140148.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10506 a         6693 c         6817 g        10404 t
ORIGIN      
        1 caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct tttgaacaaa
       61 taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt atcaacattt
      121 ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta taactccgag
      181 agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat tcacaccaac
      241 tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct atttctgctt
      301 ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg aaaattttga
      361 ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac aatgaggggt
      421 atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga acaatccctg
      481 atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg caagtagtga
      541 aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa actactgata
      601 tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag ccatttaatc
      661 cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg cagattgccc
      721 cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac tctcatgaag
      781 ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt atcctctact
      841 tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag cgtgtttgga
      901 aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat ttaagagata
      961 agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga gtgggctata
     1021 aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa tttgacttca
     1081 aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt gcttctatta
     1141 aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc tttgacgaat
     1201 gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt gttctggaac
     1261 ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt gtattggcgc
     1321 ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact tcactacatc
     1381 gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt aagccgagtg
     1441 gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga cgaacgtaaa
     1501 atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg taccccactc
     1561 acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc agaagaagat
     1621 cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc tttagtggac
     1681 gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt ttctgccttt
     1741 gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc ttctaaaaaa
     1801 aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg cgataccgaa
     1861 aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg aaaaggagca
     1921 acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac agtcaaaaat
     1981 aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt ttatcgtgta
     2041 gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat cgctcaaagc
     2101 attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg gacttctttc
     2161 atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca ggaagttatt
     2221 gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg aaaaacacag
     2281 aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc agcaaaggat
     2341 aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc ggttcaacag
     2401 ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc gaatttcaga
     2461 ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa aaaggaggaa
     2521 atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa gaaaaattga
     2581 tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat gaaaatcgtc
     2641 gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt tctgctgaga
     2701 tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa atgaaagaag
     2761 tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa aaattgaaac
     2821 aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga acagatgaag
     2881 aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt gaaccaaaac
     2941 cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc gcaaaagtag
     3001 ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt gctgtctaca
     3061 acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc actccttata
     3121 tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa gaagatgttt
     3181 tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca ttaactaact
     3241 attataatga tgaaatttta gatggtgcag atttacctat ttatttcact gcactaagcc
     3301 ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg attcgtcttc
     3361 accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct tacgaagaat
     3421 tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg ccttatcgtg
     3481 tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat gacttagaag
     3541 tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac tgtgaagatt
     3601 tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc cactatactc
     3661 atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt ttggaaaact
     3721 accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat atggggaatc
     3781 tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta ttaagaaaaa
     3841 ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg agaaactggc
     3901 ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag tattagagaa
     3961 aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc gagggtcagc
     4021 tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct agttacaagg
     4081 acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat tcggaggtgg
     4141 caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact ccaagaaata
     4201 agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta tttccgaagg
     4261 agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag tgttctttac
     4321 ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt gcttcttttg
     4381 tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt tggataaatt
     4441 gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt cctgaacgca
     4501 aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca acccgacctt
     4561 cgattccttc tggtactaat ttatttgctt cattgacact accttggaag taacggtctt
     4621 ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg aatcgacgtc
     4681 cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata cttcctagca
     4741 tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat ttgatccctc
     4801 cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca taaatagctg
     4861 tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat cctggtccga
     4921 taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct tcagccgtcg
     4981 caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct tgaatttttc
     5041 ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct gcccctgctt
     5101 ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct gctgctacaa
     5161 gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc tcgatgtctt
     5221 tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat ttttcgattt
     5281 tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg gcagtcacta
     5341 agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca aaacgcatgt
     5401 cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg attggcacac
     5461 cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc tgtggcgtta
     5521 agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt acttcatccg
     5581 cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt cttgccatag
     5641 caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt ggaatattca
     5701 acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac acgtgacttt
     5761 ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac tttgtttccc
     5821 agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat aattgatttt
     5881 ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa taccaatata
     5941 aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg catcaagtaa
     6001 taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt ttattaccgc
     6061 agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc ttagtttcga
     6121 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata
     6181 ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca agacttttgt
     6241 cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt actaccccta
     6301 gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg gataaacttc
     6361 caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt gccccgataa
     6421 tgataactac tactggatct aatactggat tgataactgg tggcaatagt gttgtcgcag
     6481 aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc ttacccattc
     6541 ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat ttgtacatca
     6601 tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag ccataagctg
     6661 gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat agaccaagca
     6721 acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt acttctgctg
     6781 gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga cgggctgttc
     6841 ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct tttacttttt
     6901 cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg tactgttgat
     6961 ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct cgtaattgtt
     7021 ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt cgtcacaaaa
     7081 gacattctac catattctac agctagtttg atagcccttt tacacattaa agcggaataa
     7141 cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac gtccagcttc
     7201 ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag tttctgcacg
     7261 gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg ctttgattcc
     7321 ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag tagctaagcc
     7381 taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga ttcctaaagc
     7441 ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt ctgcacgagc
     7501 actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt cttgtacata
     7561 atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat aaagaattgg
     7621 ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag taaattcgat
     7681 cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta cagctagttc
     7741 tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat agcgtttgtt
     7801 gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat cttctagtgg
     7861 atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa caacgtgaca
     7921 aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt ctcctttact
     7981 tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa cagttttttt
     8041 cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta ccatccctac
     8101 gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt ttgtgatcgc
     8161 attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag tcaatgccat
     8221 aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt cttttcaaaa
     8281 tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt gatatctgcg
     8341 cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt catttcaacg
     8401 atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca tcaaattgaa
     8461 tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca atctcgatct
     8521 tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc atcaaacgat
     8581 cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt tttttctttt
     8641 cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg acagttaaat
     8701 tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt aaacctaaca
     8761 gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg acaagatccg
     8821 gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct aaacgactag
     8881 ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct tctgcttctt
     8941 ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc atcatcgctt
     9001 cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt ttggacgctc
     9061 gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg cgtacgatga
     9121 tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa gatacttctt
     9181 caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt agctggacaa
     9241 cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac agtgcatcga
     9301 ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc caatgcttgg
     9361 tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc atgactaggt
     9421 gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc gcggaagtat
     9481 ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata cattgtcagt
     9541 aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg aaccgtattc
     9601 agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat caaaatcgaa
     9661 tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca atcgatcaaa
     9721 atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga ttctcttgcc
     9781 atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg cacaatactc
     9841 aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt gactagaaca
     9901 tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt agcatttcct
     9961 tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca agcacctaga
    10021 ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga tattatttgt
    10081 gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc tttcttgggt
    10141 atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga tcagagacat
    10201 tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca atcgcttgtt
    10261 ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa tagccttctt
    10321 gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc gcacgagcag
    10381 tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct gcccggtcat
    10441 gatataagga agcatcgatt cctaataatc gggttaactc agataagaag gttattcgtt
    10501 tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt aaaggtatgc
    10561 taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta aagtctactt
    10621 caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt tcatccgtaa
    10681 tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa atagcgaatt
    10741 gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg cgaaattctt
    10801 ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga ttctctccct
    10861 actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa aaatttgaaa
    10921 agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat acacagaaga
    10981 gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt attattacgt
    11041 attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc attaggatac
    11101 ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt tctggttgga
    11161 ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg tagtcgatgt
    11221 tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc atggcttttt
    11281 tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt cgtgtcaatt
    11341 cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga cgtcttaaaa
    11401 agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct aaaaagtctt
    11461 ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct tgttcaactg
    11521 cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct acacggtgac
    11581 ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg tattcagcac
    11641 gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca tcgatcatga
    11701 caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga actttcaaag
    11761 ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg cttgtaccat
    11821 ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc ggacggagct
    11881 ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt tctacttttt
    11941 ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa gaagtagata
    12001 aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat aattgatgac
    12061 gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag cgagcgccta
    12121 ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga ataatttggt
    12181 gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta taggcactat
    12241 ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct tctgtaacag
    12301 aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac cgttcgatag
    12361 caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag ttattcggac
    12421 ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt ccggctgtaa
    12481 taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca tgacaaacac
    12541 catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt ggttcttttt
    12601 cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct cgcaatgctc
    12661 ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt ttatcgatat
    12721 tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct ttagctggtc
    12781 cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt gtcagtaata
    12841 atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg tgtcctgcgc
    12901 caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg ttcctccttt
    12961 tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg tacactgtcg
    13021 ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac gagatccaca
    13081 ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc tttttctaac
    13141 aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt ttctccgctg
    13201 aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt agctacggaa
    13261 attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc cagctgtgct
    13321 ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc ttccaataat
    13381 tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat caagtctgct
    13441 tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc ttctgtttcc
    13501 cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac atactcttcg
    13561 attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc tcttaacaaa
    13621 tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt acttaaccct
    13681 tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct ttgttggatc
    13741 atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc atattctgga
    13801 taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct caatgagcgg
    13861 atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc tttgtctgtt
    13921 tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg accattcaag
    13981 aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct taacaatagc
    14041 tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac tacatcttcc
    14101 cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac aagctgattg
    14161 ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc tgataatcgt
    14221 ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc acttaaacga
    14281 acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt atcaaattct
    14341 aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa aaagtgccca
    14401 ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg ttcttgatta
    14461 aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa acaattagat
    14521 gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga tgtctctttg
    14581 cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc tttcttaaaa
    14641 aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag gagaaaatat
    14701 gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat caacgatgtc
    14761 gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt ttttgttgtg
    14821 gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct tttcttaaca
    14881 acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt aatcaactgt
    14941 cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact ttttgcttat
    15001 ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac aaaaaaagcg
    15061 gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg actacttcat
    15121 cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa actttgtcaa
    15181 cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc tactactaaa
    15241 taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt gcttaatgca
    15301 gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg ctgccctgtc
    15361 cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg gcgcttttca
    15421 cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg atggatgaag
    15481 acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat cagtatgcct
    15541 tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc cattttgaca
    15601 agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa ttccgttaaa
    15661 gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat cgtttcgttg
    15721 actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt ctgttctact
    15781 gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt ctttcgaccc
    15841 attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg gtctttggcc
    15901 aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc ttcataaatc
    15961 ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt gctttcttaa
    16021 gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt cgaattttga
    16081 aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact acccagtata
    16141 atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc atcacgaaat
    16201 tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa tacgtacttg
    16261 caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg ttccctaagt
    16321 tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat tgccataacg
    16381 ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga ttgacgccat
    16441 tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc ggatctcttg
    16501 aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt ttacgcatgc
    16561 tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt gtaaataaaa
    16621 tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca aagtaataaa
    16681 caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt ccgcaaccgg
    16741 ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg tatttcttca
    16801 cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga ctaaattgga
    16861 gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa taaaatccaa
    16921 ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc gtttgacttc
    16981 attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc gaaaatgagg
    17041 ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag aatctcccgt
    17101 atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc tcattagttt
    17161 tctttccttt tctatctctc acttacgctc ataatcatac cataattacg attgtccttt
    17221 catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg cctaagctga
    17281 aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat ttttagtact
    17341 catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt ggtatgttct
    17401 tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga catactttga
    17461 tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat tgttaaaact
    17521 tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat gcacagggat
    17581 tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa aaaagtaatc
    17641 cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc tgtatttttt
    17701 atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg tgcattagtg
    17761 gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt cgattgtgtg
    17821 gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt gaaaaaaatg
    17881 ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat ggtatccctc
    17941 gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc tgctagctat
    18001 aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct cgttgttgag
    18061 gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa aatcgttgaa
    18121 gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg cgaagaagca
    18181 gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag tccgctaaaa
    18241 aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa aggaaaccag
    18301 atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta taacccgctc
    18361 ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat tggtcatcaa
    18421 atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac ttttgctaat
    18481 gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga atatcgaaat
    18541 gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc tacgcaagaa
    18601 gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt actaaccagt
    18661 gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg ctttgcatgg
    18721 gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat tttacgtaaa
    18781 aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat cgcaggacaa
    18841 attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc ttttgctgcg
    18901 atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac gttaaaatca
    18961 ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc aaagtattat
    19021 cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt tgttcctaga
    19081 caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa aatcggagct
    19141 gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat ccagcagctg
    19201 atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt tttactaaat
    19261 taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa acgttcaaaa
    19321 gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt ttgagttttc
    19381 cacagaatca acatgcccta ttacttttat tattcttttt aactaataat aaataaacaa
    19441 acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt atgcaggaat
    19501 tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg acaggtgtaa
    19561 aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat atatcaattg
    19621 aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct actggttcca
    19681 ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa gaaacattta
    19741 ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg aattttatcg
    19801 taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc cataaccaga
    19861 tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt gctgtctcac
    19921 aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat aattctttat
    19981 tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta gaacaagcgg
    20041 ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct cgctcattaa
    20101 caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg ttcaaaacag
    20161 aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc aatcgtttga
    20221 ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta gcggcgatcg
    20281 aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta tctattcgtc
    20341 cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa gaaagcttga
    20401 gttatacagc aagcagtggc gatccattag atatttcttt caatccggat tatatgaaag
    20461 cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct gctattcgtc
    20521 catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt actcctgtac
    20581 gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga aatagaaaaa
    20641 ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt atttttgttt
    20701 aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata aatttgtttt
    20761 attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag ggatgggcat
    20821 tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg ttgaaagaag
    20881 tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac actgtgttag
    20941 tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg atggttgaag
    21001 ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat gaggctgaat
    21061 aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt ttccaagaac
    21121 ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga aagtatctac
    21181 gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat tggctggact
    21241 gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct tccattagaa
    21301 atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta
    21361 agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct ttctttagta
    21421 aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca gatcaatcca
    21481 atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa ccagtacttg
    21541 aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt gtccgaacaa
    21601 ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa gcgattggag
    21661 ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct ggaaatcgag
    21721 tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca agaacaattt
    21781 ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac gacagttggt
    21841 cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac ttatggatcg
    21901 caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga tttgattaaa
    21961 gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga gctggatgat
    22021 aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca
    22081 acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt ttatgttcaa
    22141 caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta gtagaacgcg
    22201 ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc gttcgtaaac
    22261 gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt gtttgggaaa
    22321 ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc catgtgatta
    22381 ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct gtagatatcc
    22441 aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat gctggaggaa
    22501 aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga tcttctgttg
    22561 taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat gtgtactatc
    22621 aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa acggatcgcc
    22681 atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca actgagtttg
    22741 atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc gggatgcgta
    22801 tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat tatgacggcg
    22861 ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt ccagaacctg
    22921 tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa tatacagacg
    22981 gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa ggtggaacac
    23041 atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa
    23101 agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa ggattgacag
    23161 ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa acaaaattgg
    23221 gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc atgaaattct
    23281 tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta gcttccagag
    23341 ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct ttagagatta
    23401 gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt
    23461 tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat cgagagtttc
    23521 aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc atggacaaga
    23581 tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg tttggagaag
    23641 attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat gccgatgtcg
    23701 acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg cgtcctattg
    23761 ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa caagggaaga
    23821 atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt
    23881 cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga gaaatggatg
    23941 atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc cgcgtcagtg
    24001 tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat cgagtagaac
    24061 cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat atttaatcat
    24121 gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt caatctaacc
    24181 agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc acgtgcactt
    24241 cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg catgaacgaa
    24301 ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg agatgtcatg
    24361 ggtaaatacc atcctcatgg agacattgcg atctatgagt caatggtacg gatggcacag
    24421 ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc tgttgacggt
    24481 gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc tacagaaatg
    24541 ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga tacagaaaaa
    24601 gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac aacggggatc
    24661 gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt agcggctatc
    24721 gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt attacctgga
    24781 ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag agcgtatgaa
    24841 acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat gccaaatggt
    24901 aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa attgatcgaa
    24961 cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt gcgggacgaa
    25021 tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag cgcatccgtt
    25081 atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt caatatgttg
    25141 gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga aaactatatc
    25201 gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa agcagaagca
    25261 agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga aatcattgcg
    25321 attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga acggttcgat
    25381 ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt gaccggtttg
    25441 gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga agatttaact
    25501 gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt agcagacttg
    25561 aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt cttaagtctg
    25621 gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa caatggttac
    25681 atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa
    25741 ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc tacccatgat
    25801 acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata cgaaattcca
    25861 gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat cgattcgagt
    25921 gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca ctatctcttc
    25981 tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc taacatccga
    26041 agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg
    26101 acagatggtc aatctaatat cattattgga acacataacg gctactctgt cactttcaaa
    26161 gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat acgcctgcgt
    26221 gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt attagtgatc
    26281 acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa aggccgcgga
    26341 ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc tggattgacc
    26401 actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat tatccgtttc
    26461 aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt gatgaagatg
    26521 gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc agaacaaaca
    26581 gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc tagcaagaat
    26641 aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa tttctcttaa
    26701 aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta taattagagc
    26761 ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta agagccgaaa
    26821 gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt acattattcg
    26881 tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa
    26941 agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga
    27001 aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc catcatcagc
    27061 aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca ttcgtcacat
    27121 gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg
    27181 agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa gatccagatt
    27241 tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg aatcgtaatt
    27301 ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat
    27361 cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt actggacgta
    27421 tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca gaagtagtag
    27481 ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg
    27541 gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac tctaatcaaa
    27601 catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac ccattcggct
    27661 caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat ttgataggag
    27721 ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt agactacatt
    27781 gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa aagatttatt
    27841 tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa aaaccaacgt
    27901 aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt cgttagcgaa
    27961 gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc aattcctttt
    28021 tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt atggtaaaat
    28081 caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt tatccccgaa
    28141 acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa ttttaccatt
    28201 tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc gctattggtt
    28261 gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg cttcgactgt
    28321 agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga ttgcttatca
    28381 gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca aagatcaaag
    28441 tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa aaaagaaaaa
    28501 ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa agggtgttgt
    28561 ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga acagtcagcc
    28621 ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat tagatgacaa
    28681 agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga tgaatgaata
    28741 ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac agatgatcga
    28801 tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag cagaagctga
    28861 aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa gaaccctcga
    28921 tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg gaggcgacca
    28981 agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg ggaaatctgc
    29041 taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat
    29101 ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata tggatgcttt
    29161 aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag cttacatcgt
    29221 tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa tccataagca
    29281 tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga aaaaagaaga
    29341 ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc agggcctata
    29401 tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag agtttccaag
    29461 taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc tagtttctga
    29521 gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg ccacattatt
    29581 gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg cacgaacttt
    29641 tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac aatatttatt
    29701 gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa gcgactatgt
    29761 gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata gtgttatagc
    29821 agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt tcgtcattac
    29881 aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga tcaacgtcca
    29941 aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta ctcaactgga
    30001 aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag aaaatatgaa
    30061 tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga tgtaaaagga
    30121 aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc
    30181 aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc aggacaaaag
    30241 aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc
    30301 ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga cagccgtttg
    30361 tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata caaaatcaaa
    30421 ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta cacaaacgta
    30481 cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt acaagaataa
    30541 caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa gacaaaccga
    30601 aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt
    30661 gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt ataataaaag
    30721 agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca ttttcttgta
    30781 aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca ggatcgaatc
    30841 ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct tgacgcagaa
    30901 acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg tggccatcaa
    30961 attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga cgtggttact
    31021 gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt aagttatctc
    31081 tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc gaaaatcgtt
    31141 gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt tacgaaagga
    31201 ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag tatcttggag
    31261 gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact gaactccacg
    31321 attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct accaactggc
    31381 tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat cattcttgct
    31441 gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca
    31501 aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc tcttgtaaat
    31561 cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg tcagctttcg
    31621 gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc aaatattttt
    31681 atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg
    31741 caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat tgaaggtaac
    31801 ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa aaaactggcg
    31861 aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt agaacagcga
    31921 caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga acaagatgcg
    31981 gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg agatgacgat
    32041 gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc
    32101 ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt ttcgtcgctt
    32161 tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc gagagctctc
    32221 gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta aacagatctc
    32281 caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat
    32341 cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat tttgacgttt
    32401 gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt aaacggtgtt
    32461 ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag cagttttttg
    32521 aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc ttaaacggtg
    32581 ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga gaagctattt
    32641 ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg
    32701 tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa
    32761 ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac ctctttttta
    32821 ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat aaaaataaaa
    32881 atcattcgat tttatttgaa atatgtatct attattgata caatggttca ggattaaata
    32941 atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa tggggcgatg
    33001 aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt gcacggtacc
    33061 aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat aaactgcatt
    33121 tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac ggcgttgtag
    33181 tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt gtagaaacaa
    33241 gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc aaattggacc
    33301 aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa ggaatcggac
    33361 ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta ttagataaag
    33421 aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa
    33481 tgtttgattc agaaccactt gattttgacg atatttttga agaatattat gaatacggac
    33541 aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct ttggatgcag
    33601 gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac cagggaactt
    33661 atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga agcggtgtag
    33721 ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg
    33781 atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc gaagtaggac
    33841 gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta
    33901 tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg atcgatgtat
    33961 taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc gaactaatct
    34021 accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat gaagaattgc
    34081 caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca gaaaacgcac
    34141 gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg
    34201 gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc taaggataaa
    34261 aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat ctatacgata
    34321 ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata ggctgtctct
    34381 tgaacactgt ttattcagaa taagggactg tgacaaaaac
//
LOCUS       DAZAJZ010000021        33499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000021 DAZAJZ010000000
VERSION     DAZAJZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.021"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACG86D_000982"
     CDS             59..415
                     /locus_tag="ACG86D_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN3140149.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACG86D_000983"
     CDS             405..671
                     /locus_tag="ACG86D_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3140150.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACG86D_000984"
     CDS             830..1831
                     /locus_tag="ACG86D_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN3140151.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACG86D_000985"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACG86D_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACG86D_000986"
     CDS             3055..3300
                     /locus_tag="ACG86D_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140152.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACG86D_000987"
     CDS             3398..3619
                     /locus_tag="ACG86D_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140153.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACG86D_000988"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACG86D_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACG86D_000989"
     CDS             4761..5300
                     /locus_tag="ACG86D_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140154.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6320..7489
                     /locus_tag="ACG86D_000990"
     CDS             6320..7489
                     /locus_tag="ACG86D_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3140155.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            7962..8516
                     /locus_tag="ACG86D_000991"
     CDS             7962..8516
                     /locus_tag="ACG86D_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3140156.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(8689..9126)
                     /locus_tag="ACG86D_000992"
     CDS             complement(8689..9126)
                     /locus_tag="ACG86D_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3140157.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            9174..10121
                     /locus_tag="ACG86D_000993"
                     /pseudo
     CDS             9174..10121
                     /locus_tag="ACG86D_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            10392..10827
                     /locus_tag="ACG86D_000994"
                     /pseudo
     CDS             10392..10827
                     /locus_tag="ACG86D_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            10843..11676
                     /locus_tag="ACG86D_000995"
     CDS             10843..11676
                     /locus_tag="ACG86D_000995"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3140158.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            11921..12946
                     /locus_tag="ACG86D_000996"
     CDS             11921..12946
                     /locus_tag="ACG86D_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3140159.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            13084..13209
                     /locus_tag="ACG86D_000997"
     CDS             13084..13209
                     /locus_tag="ACG86D_000997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140160.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            13234..14349
                     /locus_tag="ACG86D_000998"
                     /pseudo
     CDS             13234..14349
                     /locus_tag="ACG86D_000998"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            14432..15328
                     /locus_tag="ACG86D_000999"
                     /pseudo
     CDS             14432..15328
                     /locus_tag="ACG86D_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            15344..16168
                     /locus_tag="ACG86D_001000"
     CDS             15344..16168
                     /locus_tag="ACG86D_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140161.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            16200..17510
                     /locus_tag="ACG86D_001001"
     CDS             16200..17510
                     /locus_tag="ACG86D_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN3140162.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            17658..18917
                     /locus_tag="ACG86D_001002"
     CDS             17658..18917
                     /locus_tag="ACG86D_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3140163.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            18927..19799
                     /locus_tag="ACG86D_001003"
     CDS             18927..19799
                     /locus_tag="ACG86D_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140164.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            19811..20641
                     /locus_tag="ACG86D_001004"
     CDS             19811..20641
                     /locus_tag="ACG86D_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140165.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            20681..22864
                     /locus_tag="ACG86D_001005"
     CDS             20681..22864
                     /locus_tag="ACG86D_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3140166.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            22925..24145
                     /locus_tag="ACG86D_001006"
     CDS             22925..24145
                     /locus_tag="ACG86D_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN3140167.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            24142..25602
                     /gene="gtfA"
                     /locus_tag="ACG86D_001007"
     CDS             24142..25602
                     /gene="gtfA"
                     /locus_tag="ACG86D_001007"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN3140168.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            25679..26785
                     /locus_tag="ACG86D_001008"
                     /pseudo
     CDS             25679..26785
                     /locus_tag="ACG86D_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            27107..27316
                     /locus_tag="ACG86D_001009"
     CDS             27107..27316
                     /locus_tag="ACG86D_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3140169.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            27309..27458
                     /locus_tag="ACG86D_001010"
     CDS             27309..27458
                     /locus_tag="ACG86D_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN3140170.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            27612..28277
                     /locus_tag="ACG86D_001011"
     CDS             27612..28277
                     /locus_tag="ACG86D_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3140171.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(28561..29646)
                     /gene="xerS"
                     /locus_tag="ACG86D_001012"
     CDS             complement(28561..29646)
                     /gene="xerS"
                     /locus_tag="ACG86D_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN3140172.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            29980..30213
                     /locus_tag="ACG86D_001013"
     CDS             29980..30213
                     /locus_tag="ACG86D_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN3140173.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            30210..30617
                     /locus_tag="ACG86D_001014"
     CDS             30210..30617
                     /locus_tag="ACG86D_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN3140174.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(31754..32272)
                     /locus_tag="ACG86D_001015"
     CDS             complement(31754..32272)
                     /locus_tag="ACG86D_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN3140175.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            32639..32887
                     /locus_tag="ACG86D_001016"
     CDS             32639..32887
                     /locus_tag="ACG86D_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140176.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            33390..>33499
                     /locus_tag="ACG86D_001017"
     CDS             33390..>33499
                     /locus_tag="ACG86D_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3140177.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT        11630 a         4996 c         6145 g        10728 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
     6001 tatattttag gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa
     6061 tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt
     6121 aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag
     6181 tgatataacg aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga
     6241 tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat
     6301 tttataggag gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa
     6361 taaaggtgat ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt
     6421 gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc
     6481 aggtaattcc cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa
     6541 tttggtaatt cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa
     6601 aagaaccaat gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat
     6661 gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat
     6721 ttcaaatatg actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga
     6781 atccagatac tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt
     6841 ttcaaaagaa gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct
     6901 tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct
     6961 aaaagaccgt ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa
     7021 cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc
     7081 tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa
     7141 attagtttat caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga
     7201 taaatggaaa aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac
     7261 cttttataat ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg
     7321 ttttaacaaa caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc
     7381 tctggagaga tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt
     7441 tcataaaggt tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca
     7501 gaatgggctt ttccatttac acaaaattat tgacgctccc gacaaaatta tgatcttgcg
     7561 taaagttata aaatattaat aaaaatattt tattaatgaa aagcttggta gcacggttat
     7621 tcatatgcta atttatttca ctttatagta aggggagttc ttagtatatt gttcaaactt
     7681 aaatttcata taatttaatt atgtaaatta gctattctaa gaacttctac ggataactgc
     7741 ccgtctaaaa tttaaataag agcttagaat cttcatataa gtaaggttaa tttttttgta
     7801 caaaaaagtc ggaatattct gagttactga aatgatataa aatcataaac taaatccctt
     7861 gattttattg actttcaaaa gtttatatat ataagttaat gattatggta aaatatatac
     7921 ataatcatta actcagaata tttttaagaa gggaaacaat agtgagctat aatgtacaac
     7981 cgttaaggac aaatgaagag atacaagatt ttcagttttg gctaagaaga acaagtaatc
     8041 cggaaagaga tagtttttta tttttgtttg gaatcaataa tgggttacgt atgtcagata
     8101 ttataggatt aaaagttgga gatatacgag gcaaaagtaa gcctattatt gttgaacata
     8161 aaacaggtaa gcgaaaaccg atttttatag ataatttgag agaagaaatt ttattatata
     8221 ctgaaggtaa agaagaaaat gattggttat ttccgagtcg gcaacaaggt cgacatataa
     8281 caagggatcg tgtctatcag atatatgcag atatagcaga aaaattagga cgagatgata
     8341 taggaacaca tacattaaga aaaacttttg ggtatcacta ctacaagaaa actagagaca
     8401 ttgctacctt aatgtttatt tttaatcata gctcgcaagc aattacaaaa aggtacattg
     8461 gtataactga agatgaaata ggagcatcac ttcgtggatt taaactaggt gtataattta
     8521 aaaattttta taaacagtaa agacaaagat taaaagctat ttttatctag aacgatttta
     8581 tttcataaag ttcgcggaat aaaagctggg gttactggta cttggtatag gttcgtgtta
     8641 tataaactaa gtaattaaat aacattttta ggttggaaaa ggatgaaatt aaaaaaagag
     8701 aatcaatacg gtctcaaaaa cgcaaacgct gcggaataag aaaaatactg ataactcaac
     8761 gtttagcaga ctatctaata gggatagtct gcttttttcg tttttgacca tacttttgat
     8821 cttctgcaaa cttccttgct tttcctctgt agaactcatg aaatctgcaa aacgaattgc
     8881 cgtttcctct gtctgctttt tcgcgatatg ggtgtatata tcatccatag tggttttaat
     8941 atctgtatgt ccaagtcgaa catacacttc tttgacagac gcaccagatt caaagagcag
     9001 actacaatgg gtatgacgaa aaccatgaag tgtgatatgt tttaagttat tctctttaat
     9061 gatgattttc aaattgtgat ttacatagtc taaataaagg ggcttatttt cttctgatga
     9121 aaacaaaagt tgttcaccat cttttatagg tttgattctc atttttagat attatgcccg
     9181 cacgtattca tcacatttct attgtgaatc gctttattcg accgacattt gacttttatc
     9241 ataatatact cggtttaaaa ttactaatga aaacaattaa tcaagacgat cataccatgt
     9301 atcacctgtt tttttcagat aatcaccacc gtgtgggaac tgaactgact ttttttgaag
     9361 tacaagaagg gaacaaccag ttttttggaa ccaatacgat tgaacgaacg atactaaaag
     9421 ttccttccga agcatcattg catttttggg aaatatattt tgaaacacaa ggcgtttgcc
     9481 attatggaat cgaatcattt tctggacgac ctatcttacg ttttgaaggg ccagatgcaa
     9541 cacgattagc tttagttccc ttacgcgaat ttgaagatat tgatgattat ttcccgtatg
     9601 tcacagacca gatccctaca gagcatgcca ttttaggaat tgatgccatt caactacgcg
     9661 tacaatattc cgatgcgact gaacgagagt tattgtcggt aggctggtag cacaaagaat
     9721 acgtcagttt cttttcgggt catcaacgag tgacaatctt agaaaatcaa gacactcagt
     9781 tctatcaaga agttcagatt attcaagatc gtgagaatcc agttgccatt gaagggattg
     9841 gtagcgtcca tcatgttgcg tttggcgtag aaaataaatc tgatttacaa cgcatcgaca
     9901 aacaattaca agagcgcaat tttataaatt caggaataaa agatcgtgaa ttttttgtat
     9961 cactttatta tcgtgaccca aatcaattat tgattgaaat tgctacaggg gaaggaaacc
    10021 tagatgcaaa agcgtatgaa aatcaaagtc cacgctttga agaaattcct ttatttttac
    10081 cgcagtactt aaatttcatt cgtgaacaag tggagcaata attagctaat caaatccatg
    10141 acgaagagta agaggttgac tcataagtca aaaaataaag aaaaacgcat caaatccatt
    10201 ctaaagaatg ttgatttgat gcggttccta tttttataaa ctatcatttt cgactaataa
    10261 aactacttat gagtcagcct ctcttttttt cttgattttt atacctcttt ctaggtaaac
    10321 taaacatctg ccaaaaatac cgtgtctcat tgaaacattc tcaactattt caaaataaag
    10381 gggagtacca aatgaacgag tatattgaaa ttatccacgc aagagaaaca acttaaaaat
    10441 gtcagtctca aaattcccaa aaataaaatt agtattttta ccagcgtatc tggttcagga
    10501 aaatcatcga ttgtttttga tactattacc caagaagctg gtcgtcaact gaatgacacc
    10561 tatgatagtt ttacccgttt atttttacct aaatacagtc gtccagaggt tgatgagatt
    10621 gataatcttt ctacggcgat tgtggtcaat caaaagaaga ttggtggcag tgcgcgctca
    10681 acgttaggga cgattactaa catcaatgcg ttatttcgtg tgttgttttc atgttttgca
    10741 acacctagtt taggatatgc aaatgcttat tcatttaatg atcctagtgg aatgtgttct
    10801 gtctgtcagg gaatcggtaa gacgattgca tttttaatag gattgcttta tttttcggct
    10861 attgataatg ctgattttgt attttctgat ccatttaccg cagaagtttt gtctggaatt
    10921 gaggaacagg caaagaatca tggttatttt gtattggtgc ataatgttaa ttcagcagaa
    10981 gatgttaaaa ctattcagag aaattggcgt tttgaggggt tcattgttgt tggtgtacgt
    11041 gtaaaagaat ttttggaatt aaataacgtt tttgatggtc ctgttgttta tattgatacg
    11101 catctttcaa aagagacatt aagagaactt gaacaacaaa tgaatcgttt atttgtaaat
    11161 acaaatgatt accaagctag tacattggca gtggaacatt tgattcatta tggtcatgag
    11221 aaaatagcgt ttctttcttt tgactacatt gtaggtgaac caagtgttat tcaagaacga
    11281 catcaagctt atatcgatac attaaagaaa tatcatttaa cctttaaatc gcattggata
    11341 tataacaact ctgaatttga gcgaatttat caagaattag ctgaatttac tgcaattgtt
    11401 gttactggag attatttagc ggctaaatta gtaaaatttt taaaaaataa gaataaacga
    11461 gtgatggaac aattatcgat tattggattt gatgatatta cttttgcaga attaatggat
    11521 cctgcgttga caacaatacg actaaatcca ggtaacaagg gggcgattgc ttttcaccaa
    11581 ctgattggac tggccaaaca agaagaaata aaaaatcaag tactactatt agagggagaa
    11641 ttgatttctc gagactctgt tcacaaaatc aaataaaaaa gctctgtgaa ataagtatag
    11701 ttaacaataa aatatccgaa cgatagagag aatgaactgt gatctagcag ttcctcgtcg
    11761 caaattcact agaattagca ttatttactc taaattgttc ggatattttt tcttgttttt
    11821 aattatgatt acagtgccta tttttaacga aatttgctta ttttcaaaat gtatgattat
    11881 atgttagtaa acactttcaa atatgaaaag aggagctatt atggtaggaa ttagggatgt
    11941 tgcaaaacgg gcgggtgtgt ccattgcaac agtatcgcgc gtgctaaatg aggatccgac
    12001 actttcagta accgatgaaa ccaaacgaaa gattgcagaa tcggttaatt tttatcatta
    12061 tgtcaaaaaa agtcccgttg caaaaaattt aacaaatatt gcattaataa cgacagtttc
    12121 tgaagtagat gagttggaag atccctattt tcgaacgatt cgcagaggga ttcaagtcga
    12181 agcagaacaa agcaaagtca atttgaagaa aattattcgt ttgtcagaaa caccgttgga
    12241 gcaagacgat ttgctaaaat gtcaaggtgt gttgattatt gggcaggtgc tatcttctgt
    12301 gattgaagaa gtatcagcaa ttaattcgaa tgtcgtagtg attgatgacc ctagtattga
    12361 agctgaagta gatgcagttt atactgattt aacaaaagca acgaatcagc atttagatcg
    12421 attgtataaa aaaggacacc gaaacattgc gtttatcgga ggaggacgtg ttcgtttaga
    12481 tagccaagga aataaacacg tgtcagatga tgattatcga aaaatagcct atgagacgtg
    12541 gatgaaacaa aaaggattag aaaaatatag tcaaatttat ttagaaggct ggaccacact
    12601 tgacggaatg caggcagcag atttactttt gaaaaaacat gctaaagata agttgccgac
    12661 tgctatcatg gtaggaaatg atcctattgc tgtgggggtt tatcgttcac ttcaaaaaaa
    12721 tggtctacat attccagaag atatttcgat tgtgagtttt gataatattg aagttgctga
    12781 atttttaaca cctgcattaa atacggtgaa tgtgaataca gaagaaattg gaaaactagc
    12841 agttcgcatg gttaatgaac gaataaaaga agttagaaat gtttcgatca aagtgattgt
    12901 ttctaataat attattgtta gagaaagtga aaaaaatatc gattaaaaaa gtaaacatcc
    12961 aaaaaagggt gtttactttt ttgtttacta atatttttag taaaaaatct cgacaaaaca
    13021 ttaagtaaac agttatggtg ttgttaacaa aaagaaaacg cattcaaaac aggaggaatt
    13081 aagatgaaac atttcaaaaa aatagcttta acagggatag ctgttttaac tgggacatta
    13141 ctcgcagcat gtggtggggg agatagtaat tctaaagacg ggcaagtaac tttaagctat
    13201 gcgagttgag atactgacca agctccagga ctaagaaaaa tgttagatga atttgaaaaa
    13261 caaaatcctg atatcaaagt agaaatggag acaacgcctt gggatcaata ttgggttaaa
    13321 ttagaagctg caacaacggg aggtaatatg ccagatgtag taaccatgca ctctagcgaa
    13381 tcctataaat atatgtcaca gaatgctttg atgggacttg atgatgttat caaagaaaat
    13441 gatattgaca tgggaaactt tactgaagga attgctgatt tctatacgct tgaagacgaa
    13501 ctatacgcaa tccctaaaga tgcttctgta gtggggctat ggtacaacaa agaattattt
    13561 gataatgcag gtgtcgatta tccagatgaa acatggacat gggaaacact cagacaagct
    13621 gcaattgatt taacagatga atcaaaaggc atctatggtt ttgcatcaca tattggaaca
    13681 gagactggct actggccatt tatttatcaa aatgggggag aagtatttgc cgatgacaat
    13741 acaaaatcag gtttgaatac cccagaagca caagaagctt tagcttttta tcgagatatt
    13801 gcacttgttg atcatgcttc accaacaatt acagaattac aagatcctga taagttaagt
    13861 cgttttcaat caggtagatt agcaatgatg attgaaggaa actggtttgc accgggattt
    13921 ttgaaaaatg actatctagt tgaaaatgtt gcttctgccg tgttacctaa gggaaaagtt
    13981 aatgcaacaa ttacaaatgg acttggttgg gctgccagtg cgagtacaaa atacccagaa
    14041 gaagtgaaaa aactaatggc atttttagct agtgaggacg ccaataaaat ccaagcggaa
    14101 acgggggcat caatcccagc actaaaaggc tttgaagacg agtgggctgc ttcttcagat
    14161 acaattgatt tgagtgcttt tggaaaaatg ttggagtatg gagtccctcg tccatacaat
    14221 aaagatggat tacgagctga acaagcagaa gttcaaatta ttaaccgtat tctgtctggt
    14281 gaagtaactg ttgaagatgg aaccgctcaa attgaaaaag aagtcaatgc gatattgagc
    14341 ggaaattaaa ttagttaatt atacaatata caggcgaaag gattctattt cgtctgtata
    14401 ttaatggatg aataccaaaa agggggatat tatgaaaaat cgaatttttg gaaaatatcg
    14461 taaagatcaa gtgatatggg gctatatttt tatttttcca acggcattag gattaatttt
    14521 attaaatata tggccagcaa ttcaaacagc aattttaagt tttcaaaaaa cggtagggtt
    14581 cggtgatact gaatgggtcg gattaagaaa ttaccaaaaa atgtttgcag acggtgaagt
    14641 ttttcagtct ttattaaaca cattaattta tgcagtggtt tcgataccta gtattgttgt
    14701 attatccttg ttggtggcag ttttaatgaa tagaaaagtt aaaggattgt cgatttatcg
    14761 gacaatttat tttctaccag tcgttgctgc accatccgca gtcgcaatga tttggcgctg
    14821 gatgttcaat aatgattatg gactgattaa taacatgttg gctaaaatag gtctcgatgg
    14881 accagcatgg ttaacggatc catcgattgc gatttattct attatcattg taggagtttg
    14941 gagtgctatt ggttataaca tggtgttatt actagccggc ttataagaaa ttcctaaaga
    15001 ttattatgaa gcagcggaaa ttgatggtgc tagtccaatc cagcagtttt tcaacattac
    15061 gttaccatta gttacaccaa cattattttt tgtagttgta acaacagtta tcaatgcttt
    15121 ccaagtattt gatgttattt ttatgatgat tactcctaat agtaccgcca tgtttaaatc
    15181 acaatcatta gcctatttat tctataaaca ttcatttatt ttaaatgaca agggatatgg
    15241 ttcggcaatc gttatgttcc ttttagtgat tatattaatt attactgcaa ttcaaatgaa
    15301 gttgcagaaa aagtgggtaa actattaagg aggctatcaa attatgaaaa ataaattttt
    15361 ttcaaatgcg ccaatccata tcatgcttat tttaggtggt attgcaatgg cattaccttt
    15421 tatttggatg attttaactt cagggaaaac gttggttgaa tctacccaaa ttccacctac
    15481 aattttccct gagaaatttc agctaagtaa ctatcaacaa gtacttgatt tattaccatt
    15541 catggctttt tatatcaata catttttaat ggttgttgga cgagtgattg gctcggtttt
    15601 attttcagca atggctgcat atgcttgtgc gcgtctaagt tttccaggga aaaatatcct
    15661 tttcagctta gtacttattc aaatgatgat tcccggttcc atttttatta ctccacagta
    15721 tttattagca caaaaattgg gtattttaaa tacaattcca gcattaatat taccaggggt
    15781 agttagtgcg tttggtacgt ttttattaag acaatttttc ttaggtttac ctgatgaatt
    15841 ggaggaagcg gcctatttag aaggtgccaa tatttggacg atctttttca aaattatgtt
    15901 gccgcttgct aaatcaggat tggttgcact tggaatattc acagcgttgt ttgcatttaa
    15961 agatttaatg tggccattga ttgtcaatac ttcaattgat aagatgcctt tagctccggg
    16021 attagcaaac ttacaaggac aatatgccac gaacttccca caattaatgg caggttcgtt
    16081 attagcaatt tggccaatag ttgtgatatt catcgttttc caaagaaaat tcattgaagg
    16141 aattgctaca acaggtagta aattataaaa ctattaatta gtgattggag agtaagtaca
    16201 tgacaaaaat tacatttatt ggtgcaggaa gtactatttt tgcaaaaaac gtattaggcg
    16261 atgcgatgtt aactccaagt ctacaagatg cacatattgc attgtacgat attgatgaaa
    16321 aacgactaaa agaatcaaat ttaatgctta aaaatattaa taaaaattcg aatgaaaatc
    16381 gtgctgatat tgtttcttat tatgatcgca aagaagcgtt aagagaggct gattttgtta
    16441 tcaatgctat tcaagtgggt ggttataaac cttcaacagt gattgatttt gatattccta
    16501 aaaaatatgg gttgcgacaa actattggtg acaccatggg gattggtggg atttttagaa
    16561 ccctacggac actaccagta atgttagact ttgctaaaga aatggaagaa gtttgtccaa
    16621 atgcttggtt attgaactat acaaatccga tggcgatgtt aaccatgggt gttttaaaag
    16681 cgacgagtat taagactgtt ggtttatgtc atagcgttca agtatgcgta ccagaattgt
    16741 ttgagcattt aggaatagcc gatcaatatg acttaaatga ctttcaatgg aaaattgcag
    16801 gcattaatca tatggcattc ttgttggaaa ttacaaaaaa tggggaagat ttctatccaa
    16861 caattcgtca gttagctgca gaaaaaccaa atccacatcc agattctgta cgttttgaat
    16921 tgttgaaacg ttttggttac tatattacag aatcaagtga acacaatgca gaataccatc
    16981 catactttat taaatcaaaa tacccagaat tgattgacga attagggatt ccgttggatg
    17041 aatacttacg tcgttgtgaa caacaaatta atgattggga aagcatgcga gaggacattg
    17101 taaataatgg ggagttaaca catacgagaa gccgtgaata tgcttcatat atcatggatt
    17161 ctattacgac aggaactccg tcagtgattg caggaaatgt cttaaataaa ggcttaatca
    17221 caaacttacc gacagatgtt tgtgtggaag taccttgttt agtcgataaa aatggcgtgc
    17281 aaccaaccta tgtcggtgaa ttgccaacac aattagctgc tttaaatcgg acgaatatca
    17341 atgttcaaga actgacagta gaagccgctt taacactaga taaatcaaaa atttatcaag
    17401 cagcgtattt agatccacat ctatcatctg aattgtcttt aacagatatc aaaaatttag
    17461 tggatgattt aatcgaagca catggggact atttacctga atataattaa aaagaagctg
    17521 aaagagtaag gaattttcaa ttccttactc ttttcttagt taaatttagt aaaaatattg
    17581 acaaaacact tagtaaacag ttacattagt gttaacaaaa agaaaacgca ttcaaattgt
    17641 ataaggagga gcataaaatg aagtggtcta aaaaaattat tttagcttgt atagcactag
    17701 gagcaacgat ctctttgact gcttgtggaa aaaatcagga agatgcaagc ggtaaggtag
    17761 aaatagagtt ttttaatcaa aaaaaagaaa tgactcaaac gattcaggag attgctaaag
    17821 attttgaagc aaaaaaccct gatattcatg taaaagttgt ggatgtaccg aatgctggtg
    17881 aagtaattaa aacacgtatg cttgcaggcg atgtaccaga tgtcattaat ctttatcctc
    17941 aaagtataga attgaaagaa tgggcgaaag ctggttattt agaggactta accgaagaac
    18001 cgtatttaga gaatatcaaa aatggatatg cacaacgttt tgctattgag gatcgtgtat
    18061 atagcatacc attaaccgct aatgtctatg gtttttacta taacaaaact gcttttgagg
    18121 aaatggggat tcaagcacca gaaacttggg cagaatttga aaaaatagta gctgagatta
    18181 aagatcaaaa caaagtaccg tttgcgattg caggagcaga aggctggact ttaaatggtt
    18241 atcaccaact agctatagcg actgctgctg gtggtgaaga ggaagcgaac aatgtttggc
    18301 gtttctcaga agttaatgga attaatgctg aaagcaaaga aatgcaaaaa gattttaatc
    18361 gattagattt gttaagagaa ccaaaagctt tgcaaaacaa ctggcaaggt gccggttata
    18421 acgatacagt agttactttt acaaaaggtg atgcgttgat tatgccgaat ggttcatggg
    18481 caatgccgat gattcattca caaaatccag attttgaagt aggaactttt ccttttccag
    18541 cagatgaagc aggtcaaagt ctaacgattg gcgcaggaga cctagcctta tcagtttctg
    18601 caacttctca acataaagaa gaagccaaac gttttgtaga atatatgaca acacctgaag
    18661 cgatgcagaa atattatgat gtagatggtg caccgtgtgc tgttgagggc gttatcgaga
    18721 atactcagga ttcaccatta agtggtttaa ctgaacttgc ttttactgat cgtcatttag
    18781 tttggttagc caaagattgg aatagtgaaa atgattttta taccttaaca acaaattatt
    18841 tacacaatgg caatcaaaaa gccatgatta acgcattaaa tgctttcttt aatccaatga
    18901 aagctgacgg aaggtaggaa aataaaatga aaataaaaga aattttaaat cgttactggg
    18961 caatcatttt tgtagcaatt ccaattattt tgcagatgat ttttttctac tttccaatgg
    19021 ttcaaggtgc tttttacagt ttaactaatt ggactggtct gacttataat tttgattttg
    19081 tgggtttgaa caattataag attctttttt ctgatccgaa gtttatcaaa tcgattggct
    19141 tcactttaat tgtgacgctt tgcatgattg ctggacaaat tggttttggt attttgattg
    19201 cgcgggcgtt gaacgcaaat attaaaggga aaacattctt tagagcatgg ttcttttttc
    19261 cagcggtctt atcgggcgtg gttgtttctt tagtatttaa acaaattttt aactatggtt
    19321 taccaaaaat tggtagtatg ttaggcattg aatttttaca aaccagtctt ttaggaacaa
    19381 catggggagc gatttttgca gtaatttttg ttttactttg gcaaggaatt gccatgccag
    19441 tcatcatttt cttagcgggt tttcaaagta ttccacaaga aattattgaa gctgctagaa
    19501 tggatggggc gaatgataag caaatttttt ggaatattga aatcacctac ttattaccaa
    19561 gtatttcaat ggtatttatt atggcattaa aaggtggctt aacagcattt gatcaaattt
    19621 ttgctttaac tggtggtgga ccaagcgatg ccacaacatc tttggggtta cttgtttata
    19681 attatgcttt taaaaacaat cagtttggtt atgcgaatgc tattgcttta gtcttattta
    19741 tcttaattgg gattgtttca ctggcacaat taaaattatc gaaaaaattt gaaatctagg
    19801 aggtttccca atgaaaacat caaaagaaaa aaatatttct aaatacttgc ttttattatt
    19861 agggggcttc atgattttag ttcccttagt ttcaacgcta ttcggttcat ttaaaacaac
    19921 acccgatatt atgaataatt tctttcgttt tcctaatcca gcgactttgg aaaatttccg
    19981 aagattgtta gcggatggaa ttggtcatta cttttggaat tcggcaatca tcacaatcat
    20041 tgcagtagtt gctgtgacag caattattcc tatggctgct tattcactag caagaaaaat
    20101 gactcgtggg aaagcatacg gattgatgta tttattttta attttaggaa tttttgttcc
    20161 tttccaagta attatgattc cgattaccgt tatgatgagt aaaattggtt tagcaaatat
    20221 gtggggatta atcttactct atctcactta tgccgtacca cagacactat ttttatatgt
    20281 tggttttatt aaactaaatg tgcctatcag tctggatgag gctgctgaaa ttgatggggc
    20341 tagtcacttt acaacttatt tcaaaattgt tttcccaatg ttaaaaccaa tgcatgcgac
    20401 gactattatt cttaatgctc tatggttttg gaatgatttc atgctaccat tattaatttt
    20461 aaatcgtgat tctaatatgt ggacactacc attgtttcaa tataattata cgggacaata
    20521 ttttaacgac tatggtccaa gttttgcttc ctatacagta ggaattgtca cgattacact
    20581 agtttatctt gctttccaaa gaagtattat agctggaatg agcaatggag cagtgaaata
    20641 accaataaat tattctcatt ataaaggagt tcataaactt atgattatat ttgacaatca
    20701 cacacaaaca tttcatttac aaaataaaaa aattagctat ctattaggaa tcgaagaaaa
    20761 taattattta acgcatcttt attttggaaa aaagattacg cactattctg gtgggtatcg
    20821 ctacccccga acagatcgtt ccttctcacc caacaatgca aatgcgcaag atcgtttatt
    20881 atctttagac acattaccat tagaatttcc aggatatgga catggtgatt ttagagaacc
    20941 ggcgcataat attaaattag gaaacggtag tcgtgtaaat gattttcgtt atgatagcta
    21001 cgaaattcaa aaaggaaagc ccagcttagc gggattgcca gccttatata cggaagatga
    21061 tgatgaagct gaaacgctga ttattcaatt gaaagacgcg attagtggat tgactttaca
    21121 gttgttctac acaatttatc aaaatcacag tgtaattatt cgttctacgc aattagtaaa
    21181 taatagtgat gaaacagtgg agattaaccg cttagctagt caatcaattg attttccaaa
    21241 tcgtccactc gagttaattc atttaaatgg tacctgggca agagaacgtc aaatgactag
    21301 agaaaaaatt catacaggga taaaagtatt agatagtaaa cgcggatcaa gcagccatca
    21361 acaaaatccg tttgttgctt tagtagatgc caaaacagat gaatttcaag gagaggcata
    21421 tggatttaat ttagtttata gcggtaatca cgaaacggtg attcaaaaag atccatttga
    21481 ccaaacacgg gtcattatgg gaattaattc atttaatttt ggttggcaac taaaaactgg
    21541 cgactcattt caaacgccag aagtcgtgat ggtttatagt aatgaaggtc tgaatacgtt
    21601 atcacagacg tatcatgaat tgtataatca ccgtttaatt cgtggaaact atcgtttaaa
    21661 agaacgtccc acgttaatca acaactggga agcaacgtat tttgatttta acgaagaaaa
    21721 aatcatgaat attgtcgatg atgcgaagaa cttgggcatt gaaatgtttg ttttagatga
    21781 tggttggttt ggcggtcgtg aaaatgactt cacttcatta ggtgattggg ttgaaacaga
    21841 aggcaaactt gcttgtggtt tggaaaattt agccaagcaa gtgcatgata acgggatgaa
    21901 atttgggatt tggtttgaac cggaaatgat ttctgaagat agtgaactat ttcgagcaca
    21961 tccagattgg gcattgggta ttccaggtcg tgaccgctca attagtcgaa atcaatatgt
    22021 tcttgatttt tcaagacaag atgttcgtga taatatctat caacaaatga cggcgatttt
    22081 tgatcgtgta ccgattgatt atgtgaagtg ggatatgaat cgaaatatga cagaagtcta
    22141 ttctgtcttg ttggactcaa cgatgcaagg tgaagtggct catcgttata tgttagggtt
    22201 atatgaattt ttagaaaagt taacaacgaa ttatccaact attttatttg aaagttgttc
    22261 gggtggaggc ggtcgttttg atgcaggttt cttgtattac atgccacaaa cttggacgag
    22321 cgacaatacc gatgccgtgg ctcgtctgaa aattcaatat ggaaccagct tagtgtatcc
    22381 aatttcaagt atgggtgcgc atgtttcagc agtcccaaat catcaaacag gaagagaaac
    22441 gagtttaact attcgtggca atgctgcgat ggctggtgtt tttggttatg agctagatct
    22501 aacgcaatta acttctgaag aaaaagcaga aattaaagaa caagttgcct tttataaaaa
    22561 gcatcgccaa cttttgcaat atggaacttt ttatcgcttg gaaagtccgt ttgaaggcaa
    22621 tgatactgca tggatgtttg tctcaaaaga ccaaacagaa gccatggtct tttatttccg
    22681 agtattagca gaagctgcgc atccattagt cacattgaaa ttagcaggat tagcagaaga
    22741 agcaacttat caaatggaag atgtaacatt tgctggcgat gaattgatga atctcggttt
    22801 ttatattgac tcagaactac atggtgatta tgcgacacaa cgcttttatt tgaaacagat
    22861 atagtagctt gaatttacaa tataagtgca acaccaaaag agactcattc caaacgaaga
    22921 aaatatgttt tatttacacg catttactaa aaagcagcct gatttgaatt gggaaaatga
    22981 agaagtacgt gaagaaatct atgcaatgat taactattgg ttagataaag gactaggcgg
    23041 atttagaatc gatgcgatat taaacatcaa aaaaaaacta gagtatggca tctttgaatc
    23101 tgatggagaa gatgggttag ccttcattgg gccctggatt cttaatcagt cgggcattga
    23161 agtgtggtta aaagaaatga gagagcggac atttaaacca cataatagta tgacggtagc
    23221 agaagccgat gttcctaatg aacgtttaga tgaatatatt ggtgaagatg gctattattc
    23281 gatggttttt gattttagtt atacggatat tgatgtacct gaaactggtg aatggtttaa
    23341 agatagccaa tggacatgga cggatatgcg taccaatatt tttacgaatc aattagttac
    23401 gcaagataaa ggttggggcg ccttatattt agaaaaccac gatcaaccac gttcaattaa
    23461 taaatatatt ccagaagaat ggatcaatga ttatagtaaa aaaatgttgg cgacgctttt
    23521 tatgttacta agaggtaccc cttttattta tcaaggacaa gaactaggaa tgaccaacat
    23581 agaaatggca tcattagaag attttgatga tgtagctacg cattctcagt ataaacgggc
    23641 attagaatat ggattggcac cagagcaagc attgaaagct gtcaataaac gaagtcggga
    23701 taattctcgc acacctatgc aatggaatac gcaaaaaaat gccagattct caacttctga
    23761 taatgtatgg ttaaaagtaa atcctaatta tccagaactc aatgcagaag ctcagttaac
    23821 caatcaacag tcggtattca attattatca tcaattaatt aaattaagaa aaaatagtgt
    23881 atacaaagac gtgctaatct acggacaatt tctaccagtg aaagataaaa atgaacatat
    23941 tttgctttat gaacgccaat tagaagataa aatcgtatta gttatcttaa acgttacagc
    24001 aacagaacaa gaatattatg tagatgaaca gtaccaacaa gtattaatca ataattatga
    24061 agatgtttta ttagatcaaa gtcgaaaatt gcgtttacga ccgtttgaaa gtattgtatt
    24121 agctaattat ggaggacaat tatgaaaatt aaaaacgaag caatgctcat cacctatgca
    24181 gatagtttag gaaaaaacat gggcgaatta aaacaagtct tagacaaaca tttacaaggt
    24241 gttattggtg gtgtacatct gttgcctttt tttccatcga caggtgatcg aggctttgcg
    24301 ccgagtgatt atacgacagt tgatccagct cttggaacat gggaagatgt ggaagcatta
    24361 ggtgaagatt attatttgat gtttgatttt atgattaatc acatttctcg tgaatcaaaa
    24421 tttttccaag atttcaaaaa aaaccatgag cagtcaccat acaaagaaat gtttatccgt
    24481 attcatgaat ttttcccaga aaatcgacca acacaagaag atattgattt aatctacaag
    24541 cgtaaagaca aagcgccgtt tcaaacggta gaatttgcgg atggtacgac agaagaggtt
    24601 tggaatacct ttggggaaga acaaattgat ttagatgtga ccaaagaagt tgtgaaagaa
    24661 tttattcgag aaaccatcaa agatatggcg aaccatggct gttctttaat tcgattagat
    24721 gcgtttgctt atgcgattaa aaaattagat accaatgatt tcttcgtgga accagatatt
    24781 tgggaacttt tagacgaagt gcgtcaagaa gcagcgaaat atgacgtcga attattacca
    24841 gaaatccatg agcattattc cattcaaatg aaaattgcca atcatgatta ttatatctat
    24901 gacttcgcct tgccaatggt cactctgtat tcactttata gtggaaaatc agaacgttta
    24961 gccaattggt tgaaaatgag tccgatgaag cagtttacaa cattggatac gcatgatgga
    25021 attggtgttg tggacgctcg tgatttatta acagatgaag aattggatta cacctctgag
    25081 gaattgtata aagtcggggc gaatgtgaag aaggtctatt caagtgccaa ttacaataat
    25141 ttagatattt atcaaattaa cagcacctat tatagtgcat taggcgataa tgaccgtagc
    25201 tatctgttag cacgtgcgat tcaagtattt gcgccaggga ttccacaaat ttattacgtt
    25261 gggttattag caggaaaaaa tgatattgaa ctgttggaag caacgaaaga agggcgtaat
    25321 attaatcgtc attactatga tttagccgaa attgaagaag aagtgcaacg gccagtgatt
    25381 caacaattat ttgcactttt aaaatttaga aatgaatctg cggcgtttga tttggatgga
    25441 acgattgaag tagcaacgcc atcagaatca gaaatagtga ttacacgtaa aaataaaaac
    25501 ggtagtgata gtgcaacatt aacggctaac ttggatacga aagaatttat cattgagtct
    25561 aaagatataa aaattttcac tagtgttgca ttaaaatttt aacctattcc atagaacaaa
    25621 aaatccttta tactagcact tggatgactt atccaagacc aataaaaagg actcatacat
    25681 ggataagtat aaaacaaata cagcatttaa taaatggttt tctgccataa aattaggaaa
    25741 actttctgtc aactcccaaa aatgtatttt tgattacaat tcgtatcgta aaaaactgtc
    25801 ttttgaagct gtgctcaagc tctttctcta cgcagttaat gaagaaaaag aaagtttgcg
    25861 tgatttgagt acagctctta tcgaccgatc gctccaacta gaaaccgatg tgtcatcgat
    25921 tagtcatacc caattgtctc gagcatttaa tgcacttgaa cctaaagtgt tagaagaaat
    25981 ttttcaacaa ctgttagaaa aagttcaaca tcaaatcaaa ccaaccaagc gaaatcatct
    26041 gtatctgatt gattccagta cgttttcgct tagtttaaaa cgtcataagt gggcgacttt
    26101 ccgtcaaaca aaatcaggtg tcaagttaca cttaaactat tgttatatgg acgatactac
    26161 gatgtatcca actgatttta ccttaacaaa tgcccatgaa catgatctca atcaactacc
    26221 agtattggtg aatcaaccag aggcaactta cgtgtttgat cgtgggtatt aggactttga
    26281 aaagatggat cagatgcatt gggatggcta tttcttcgtc actcggatta aaaaaaacac
    26341 caaagttcac gtgatagata ctctggaaac atcgccagaa acagaaattc tacgagacga
    26401 gctggttcgt ttaggatcta aaacctatct aaccgccaat ttccgactag tcactgttca
    26461 agataagaac ggacgtgtgt ttcaatttat caccaatcga atggacgtct cttcaaaaga
    26521 aatctctgat atgtatcacg ctcgttggca aatcgaattg tttttcaagc acatcaaaca
    26581 acacatgacc attaaaacct tcttttctca aagtgaaaaa ggcgtgcaga atcaattgat
    26641 tttaacgatg atttccgctc tcctcacgtt tctcatcaaa ttggagacga aaacggaaaa
    26701 atcagtgttc caaatcaaac gattctttcg ttatttactg tttcagccct ttgaatgttg
    26761 tctagaaaaa ttaattccca catgagaaaa aagaaatttg gctcgttact tttggttttc
    26821 agttgaataa cagataaaaa attttctgga aaaagtcatc ctctgcgtca gcacagtttt
    26881 ttttgactta ttttcagagg aaatagtttt cagaatattc taaaatgttt tatgcaacac
    26941 tagtgatatt ttttagaatt agatttctaa tgtgcatttt ttgagtatta aggagcaagt
    27001 atacaaagat ttataaagct gattaaattt gaataaaaat atgcaggaac gctactattt
    27061 ttagtagtgt tcttttttat atttgaataa aaaatgtggt ggttaaatgg tggttataac
    27121 aaaaaatgaa ttaatgaaat taggttatgg gaagtatcag tctgagagaa tcatacgaca
    27181 ggcaaaagca attatggctt caaaaagcta tacctattac gaaaatagaa gattaggact
    27241 agtacctgtt gaatctgtag aagaagtttt aggtatgaag atagaggtag aggaggattt
    27301 tcagaatcat ggttaatttt aaaaaagata aaaatgggaa atatcatttt actgctagtt
    27361 tgggattcga caaacgaaca gggaaaagag tacaaaaaaa cgtagagggg ttaaaacagt
    27421 taaagaagaa aaaaagcgta taccgaactt gtttctaatt atggaaaaga agcagaaact
    27481 tctcaaagta ctatgttgtt tgaggagttt ttttataaat tattcctacc ttattatcaa
    27541 gggaaagtag aagaaagaac atataaaaat cggttatcta caatcaaaaa aacttttctt
    27601 atttctttaa aatggagtta agagaaatac gtccaattga cattcaaaag tggcaaaata
    27661 atttattgag caaatataaa aattcttatg taagaaacat gtatgggatt tttcagatgt
    27721 cattagatcg agcagttgtt ttgggactac ttgaaaaaaa tcctgcaaaa gttattggaa
    27781 atgtgaagaa aaggaaaact acagttgatt tttggacaaa agaagaattt acaaaagtta
    27841 tttcaacttt ttatattgat gatttgtacc aacattttgg ttttatttgt atgtggttac
    27901 tttttatgac cgttatgcgt ttgggagaag ccactgcatt aacttggaaa gatattgatt
    27961 ttgaaaataa aaacttgcgt gtgaataaaa cactctatta taagagagcc aaaacctatg
    28021 agtttgtaga acccgaaacg gcttctagta ttcgtacaat cgaactagac gacaatacaa
    28081 ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat aggaggcatg tcgtttgtac
    28141 tttcttataa tggaacacct actcagaaat atacaatccg tcatattatg agaagacatg
    28201 caacgatcgt tggtgttcat ataatccgca tacacggatt aaggcatagt catgcttctt
    28261 tgcttatatc acaataaaaa gatgcacttc gaattaaaga aaggttaaaa catgctgatg
    28321 tacaaacgac tttaggaact tatggtcatt tgtatctaaa ttcattgaga gagataacaa
    28381 atgatcccac agattatatc caaactaatt tttctaaaat caaaccgaca cctatttacg
    28441 ataataaaaa ataaatgata aacaaaagta ggatcacacc aaaagaaaca cactagaaaa
    28501 actataatac aggcttttct agcgtgttgt ttcttatttc cgcctattta tacattatac
    28561 tcataaagca tttaatgcat cgatcgtagc cgcattgtct atatgggtat ataaagttgt
    28621 tgtattggta cttgaatgtc ccaattgttg ggcagttaat accaatgaat ttgtctgttg
    28681 gtagagtcta gtagctaatg tatgacgcag tttgtgaggg ctgacttgaa ttctaaagcc
    28741 ttttgaatat tttgctacca tccgttcgat tgcaataccg gatatacgct tggcttctcc
    28801 ttggtaaatt gctagaaata aagctttttc gttttctgtt actttatatc gttctttccg
    28861 aatagctaga taattagcta aatattccat tgcgaatgct gctatattga cagcatctct
    28921 tttatttcct tttctagtta ctaccacatt attcttatct aaagataggt catctaaatt
    28981 aatatttata acctcggaga gtctcaatcc agatcctaaa atcagtgcat tgatcgccag
    29041 gtctctttct acatctcgtt gataatagat caaagctcgt ttactcaaag tatttttata
    29101 ttcattggca atataatcta gataattttg tgtatcctta tctagaaata attttttctc
    29161 aatggcattg gcacgtgcag ctaacgtagt tttatctgtg atggttgcta cttgatccat
    29221 aggattattg tctaagtatg gttttcctag aggattattg gaacttttcg aaagatattt
    29281 aaatagaacc tttaatgccg tgatatttct attgatagta gaagtagata gacctaagtt
    29341 attattatct ttgagcttag tttttgttgt ctcttttttt acattttctt ttgtgcgacg
    29401 tttcaaatga gatttgtaca agtttaggtc ataggcagtt aactgtctta tcgaggtgat
    29461 agaagtatca ggaacgatag actcactcag aagccattca taaaagagct tataattttt
    29521 tagatattct aaagtggttc gaggtgattg atctttatcc tcacgatctt gaacaaaatc
    29581 ttttatataa tcgggcatat gaagtatgat tgtatctatt ttttgatata tttctgtatt
    29641 tttcatgtca ttcatccttt caataagtag gggagaagag tatctctata attggactat
    29701 accaattata gaatgttttg tcaattggta tgctctaaaa tttattaatt tgtttcaaaa
    29761 aaagcatata aacaggtata cgtttaataa tttaaaataa atggtataca taaataggga
    29821 aggaaataga agggagcaat ctaaattttt tgaattttct taattaatta tatagaaaga
    29881 acaatctttc tatagagtta gacatgagag ggactatgct tgatttaaaa tcaacaagcg
    29941 tatatactgt atatacaaag aaaaaggagg gagaagacta tggcaattaa agaaagaaaa
    30001 tttagggaag tggggaactc agtagtatta actatttcaa aggagtttct taaagaaaat
    30061 gggttaaatc ccggagatac ggttttttta gatgaagaaa aacttcgagc ggcaattaca
    30121 aaaaaacaag ccttatctga tgaaattgat ttagcaatat ctcaagcatt aactgaatat
    30181 gatactactt ttagagagct tgtcgataaa tgagtattca ttacctagaa gccaaagata
    30241 ttataaaaat gaatgtaatc caaataaaaa aatactctcc taatgaaccc cttggtgtaa
    30301 aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg agcctcagct tttggtgaag
    30361 aggtttatcc ttcgatttat gaaaaagcag ctatcctact tatacaattg ataaagaaac
    30421 atccttttca caatgcaaat aaacgtactg ctttcttagc tacttttgtc tttttgaaaa
    30481 tcaatggaca gcttttagct attgatcaaa aagatgctgt aaatttagcg gtatatattg
    30541 ctacgtatga taaggatttc gatcaactaa aatatgaagt gatcaacact ataaagacac
    30601 atacgcagcc catttaagga gttactgtat gtgtctttat agggttatta gctgctatag
    30661 cgaaataatt gaagtaaatt acaaatagct ataattcaaa tccaactata tagaaaacgt
    30721 aaattttcta tatagttgga ttaagataac gactatttat tttatcatat ttttactata
    30781 ccctatactt ttacttggta tatttatgaa taatcgcaaa tttaccccta tactactagt
    30841 ttgtaagtta gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca
    30901 acttataaat tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa
    30961 gtttgttgac gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa
    31021 aacttatgaa atgaaagacc ttttacatct tgcagaaatc gttactggga ttaaactaag
    31081 caataaaaac taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg
    31141 ataaatgacg atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc
    31201 ttcagtatca gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact
    31261 gctcaaaatc tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat
    31321 aaaccataag cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag
    31381 atataattac cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg
    31441 gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct
    31501 gctagtctcc taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg
    31561 agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg
    31621 gcattctagg ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt
    31681 atgtagtcgt atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc
    31741 agtaaacggg aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc
    31801 caaatggagc aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg
    31861 ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat
    31921 caatcatcat gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg
    31981 gtagatgtgc ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa
    32041 attgatcaga aggcgtttta acagataatc catttctttc atcattttcc cattttagaa
    32101 ttaaattcgt ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct
    32161 caactctttt atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat
    32221 aagtcctatt tatttctttt tttaattctt ctttattttt atatgttctc attatattac
    32281 ctccataaat tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa
    32341 tccgttctac aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta
    32401 taaaatatcc caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata
    32461 cgaatatatt tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata
    32521 tcaaaatcat gttaactact atcaattact aataaaaact accactttaa gcgttaagaa
    32581 ctgttaagtt tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt
    32641 ggtaaaactc aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt
    32701 gggtaaaaaa tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat
    32761 gaaacagcta caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt
    32821 gccatattca cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc
    32881 tttttaggaa attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt
    32941 ttcaccagat attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat
    33001 tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa
    33061 aattaaggga tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt
    33121 ttaaacaatt atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat
    33181 gactttctta ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg
    33241 tttacgagta ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact
    33301 acccccactt agcttggata ggctacaatt aactagacag aaaaattaag gtgtgtagac
    33361 tagaagaaaa cataccagga ggaattttta tgtctaagag aacacgaaga actttttcac
    33421 aagaattcaa gcaacaaatc gtcaatcttt acttagctgg aaagccacgt gtagaaatca
    33481 ttcgagaata tgaactaac
//
LOCUS       DAZAJZ010000022        33356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000022 DAZAJZ010000000
VERSION     DAZAJZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.022"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..1770
                     /gene="rny"
                     /locus_tag="ACG86D_001018"
     CDS             214..1770
                     /gene="rny"
                     /locus_tag="ACG86D_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN3140178.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1849..2655)
                     /gene="proC"
                     /locus_tag="ACG86D_001019"
     CDS             complement(1849..2655)
                     /gene="proC"
                     /locus_tag="ACG86D_001019"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN3140179.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2796..5369
                     /gene="mprF"
                     /locus_tag="ACG86D_001020"
     CDS             2796..5369
                     /gene="mprF"
                     /locus_tag="ACG86D_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN3140180.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5523..6374
                     /gene="ispE"
                     /locus_tag="ACG86D_001021"
     CDS             5523..6374
                     /gene="ispE"
                     /locus_tag="ACG86D_001021"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN3140181.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            6619..7557
                     /locus_tag="ACG86D_001022"
     CDS             6619..7557
                     /locus_tag="ACG86D_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN3140182.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7580..8260
                     /locus_tag="ACG86D_001023"
     CDS             7580..8260
                     /locus_tag="ACG86D_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3140183.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8233..9057
                     /locus_tag="ACG86D_001024"
     CDS             8233..9057
                     /locus_tag="ACG86D_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN3140184.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9224..10042
                     /gene="purR"
                     /locus_tag="ACG86D_001025"
     CDS             9224..10042
                     /gene="purR"
                     /locus_tag="ACG86D_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN3140185.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10545..11918
                     /gene="glmU"
                     /locus_tag="ACG86D_001026"
     CDS             10545..11918
                     /gene="glmU"
                     /locus_tag="ACG86D_001026"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN3140186.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            12012..12983
                     /locus_tag="ACG86D_001027"
     CDS             12012..12983
                     /locus_tag="ACG86D_001027"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN3140187.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      12989..13091
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13157..13726
                     /gene="thiT"
                     /locus_tag="ACG86D_001028"
     CDS             13157..13726
                     /gene="thiT"
                     /locus_tag="ACG86D_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN3140188.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13807..14580)
                     /locus_tag="ACG86D_001029"
     CDS             complement(13807..14580)
                     /locus_tag="ACG86D_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140189.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14847..16547
                     /locus_tag="ACG86D_001030"
     CDS             14847..16547
                     /locus_tag="ACG86D_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN3140190.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16517..16735
                     /locus_tag="ACG86D_001031"
     CDS             16517..16735
                     /locus_tag="ACG86D_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN3140191.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16755..17645
                     /locus_tag="ACG86D_001032"
     CDS             16755..17645
                     /locus_tag="ACG86D_001032"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN3140192.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17663..17947
                     /locus_tag="ACG86D_001033"
     CDS             17663..17947
                     /locus_tag="ACG86D_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140193.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18434..19048
                     /gene="gmk"
                     /locus_tag="ACG86D_001034"
     CDS             18434..19048
                     /gene="gmk"
                     /locus_tag="ACG86D_001034"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN3140194.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            19053..19358
                     /gene="rpoZ"
                     /locus_tag="ACG86D_001035"
     CDS             19053..19358
                     /gene="rpoZ"
                     /locus_tag="ACG86D_001035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3140195.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19611..22019
                     /gene="priA"
                     /locus_tag="ACG86D_001036"
     CDS             19611..22019
                     /gene="priA"
                     /locus_tag="ACG86D_001036"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN3140196.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            22034..22525
                     /gene="def"
                     /locus_tag="ACG86D_001037"
     CDS             22034..22525
                     /gene="def"
                     /locus_tag="ACG86D_001037"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN3140197.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22518..23456
                     /gene="fmt"
                     /locus_tag="ACG86D_001038"
     CDS             22518..23456
                     /gene="fmt"
                     /locus_tag="ACG86D_001038"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN3140198.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23456..24814
                     /gene="rsmB"
                     /locus_tag="ACG86D_001039"
     CDS             23456..24814
                     /gene="rsmB"
                     /locus_tag="ACG86D_001039"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN3140199.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24827..25567
                     /locus_tag="ACG86D_001040"
     CDS             24827..25567
                     /locus_tag="ACG86D_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN3140200.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25564..27633
                     /gene="pknB"
                     /locus_tag="ACG86D_001041"
     CDS             25564..27633
                     /gene="pknB"
                     /locus_tag="ACG86D_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN3140201.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27798..28697
                     /gene="rsgA"
                     /locus_tag="ACG86D_001042"
     CDS             27798..28697
                     /gene="rsgA"
                     /locus_tag="ACG86D_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN3140202.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28710..29360
                     /gene="rpe"
                     /locus_tag="ACG86D_001043"
     CDS             28710..29360
                     /gene="rpe"
                     /locus_tag="ACG86D_001043"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN3140203.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29361..29999
                     /locus_tag="ACG86D_001044"
     CDS             29361..29999
                     /locus_tag="ACG86D_001044"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN3140204.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30154..31008
                     /gene="mreC"
                     /locus_tag="ACG86D_001045"
     CDS             30154..31008
                     /gene="mreC"
                     /locus_tag="ACG86D_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN3140205.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            31012..31521
                     /gene="mreD"
                     /locus_tag="ACG86D_001046"
     CDS             31012..31521
                     /gene="mreD"
                     /locus_tag="ACG86D_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN3140206.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            31776..33350
                     /locus_tag="ACG86D_001047"
     CDS             31776..33350
                     /locus_tag="ACG86D_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN3140207.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        10993 a         5968 c         7218 g         9177 t
ORIGIN      
        1 catttatttt aaaaaccatc gtttaatgaa taagtagaaa gccgtttctt tgtttattta
       61 aagttgacac tacggggtac aaacattaga ataaagttgt gcatgttttt tgtacacata
      121 tatgcgaaga tgcatattga cgagtttaga ataatttatc atagaagctg tatagatgat
      181 aaaaatgaaa aagatagtat ggaggtggat tttatggtat tcaacattct cctcgctatc
      241 atcggtttaa ttgtcggtct tggtttaggg tttatggtcg caaagtcacg tcacgagaaa
      301 gaaatagctg gtgcacaatc atcagctgct ggaatcatcg atagtgcaaa aaaagaagca
      361 gaaactctaa aaaaagaagc attgttagaa gctaaggaag aaaatcagaa ataccgttct
      421 gaagttgaaa gtgagctgag agaaagtaag ttagaattaa aatctcaaga gaatcgtttg
      481 attcaacgcg aacaaacgct gaatcgtaaa gatgactcgt tagaaaaacg cgagaactca
      541 cttgaaagta aagaagaagc acttagttca aaacagcaat taattgaaga gcgagaaaaa
      601 gacgttgaaa aaatgattga agatcaacaa aaagaactag aaaaaattgc tggtttatca
      661 aaagaggacg cacgagatgt catcatgaaa tcgactgaag aagagctgaa tcatgagtta
      721 actctaatgg taaaagaatc tgaacagcgt gccaaagaag aagcagatcg caaagcgaaa
      781 aacttattat cattagcgat tcaacgctgc gcagcagatc aagtttcaga aacgactgtt
      841 tcagtcgtta cccttccgaa tgatgaaatg aaaggtcgta tcattggacg agaaggtcgt
      901 aatattcgaa cgcttgagac gctgactggt atcgatttaa tcatcgatga tacaccggaa
      961 gcagttgttc tttccggatt cgatccaatc cgaagagaaa tcgcacggat gaccttggaa
     1021 aaactgatac aagatggacg aatccatcca gcgcgtatcg aagaaatggt cgagaaatct
     1081 cgtaaagaaa tggatgaacg tattcgcgaa tacggggaag aggcagcgtt tgaagtcggt
     1141 gctcatacac ttcatccaga tctaatcaag atcatcggac gtctgcgatt cagaactagt
     1201 tatggacaaa acgtcttgaa gcattcggtc gaggttgcaa aattagctgg tatcttagca
     1261 gcagagcttg gcgaagatat ccaattagca aaacgtgccg ggttgctcca tgatattggg
     1321 aaagcacttg accatgagat tgaaggatct cacgttgaga tcggtgcgga attagctgcc
     1381 aagtacaaag aaaacccagt agtaatcaac gcgatcgctt ctcatcatgg agatgtggaa
     1441 gctacttctg ttatctctgt ccttgtagct gcagcagatg cgctctcagc agcaagacct
     1501 ggtgcgcgaa gtgaatcatt agaaaactac atccgtcgtt taagaagtct ggaaaatatt
     1561 tcgaacagct tcgccggtgt agaatcaagt tacgcagttc aagctggacg cgaagttcgg
     1621 gtaatggtca agccagaaga aatctcggat ttagacgctg ttcgtttagt acgtgatatt
     1681 cgtaaacgaa tcgaagaaga actggattat cctggacaca tcaaagtaat cgttattcgg
     1741 gaaactcgtg caactgatta tgctaaataa taaaaaatac aaaagaagag ccgcggcaaa
     1801 agccacagct cttctttttt tgtaaggcac gaaaaaacat tgctatcatt attttttatt
     1861 caacatctca agatctttct cgatcgtcgt attgattcct ttgatgattg ctgggatgaa
     1921 tcctgcttct tctaaggcaa ctacacctgc aacagttgta ccgccaggag aagaaacttg
     1981 gtctacaagt tcccatggat ttttatcact tgaaaggaat ttttgacaag aacccataat
     2041 tgcttttgca gcaatctcag tggctgtctg tttggacaaa ccattcagta caccagtccg
     2101 tcccatcgca tcgatcagca tgtaaacgaa tgcgggtgag ctgccagcaa ggccaatgaa
     2161 tgtactgaaa tctgattctg ctaaaggata gacgctgccg attgcttcaa aaatcatcgt
     2221 agcttgctga agctgttcct ccgtggcagc ttcatttttg caaatcgcag aaacgcctgc
     2281 tcctacgctg acattgacat tcggcatcac acgtacgatg gaggcctgag gatcttcaag
     2341 cgctgtttct aaatctatta atgattttcc tgaagcaata gaaatgaata agactttctg
     2401 gctatattct cttagttctt ccaatacttt ttcaatgatt tttggtttca cggctaaaag
     2461 aacgatatct gattgtgatg caacttcctg attacttttg catgcatgga cgccagtttc
     2521 agcagcaaat ttttgtgcat tttcaaaatg tccactggaa acgaacacct cttctttcga
     2581 gactgttcct ttttttaata aaccatcaat aatagcttgt gccatattcc ctgtgccgat
     2641 aaatcctact ttcatgttat cctctccttt agcattagga tatctttctt tttctatcat
     2701 cgcaagcaaa gaacaggtct aaagtggaaa aattaaaata aataagataa cattggggta
     2761 tgacacaaaa gtatacaagt taggaatggg aaaatgtgaa aaataaacga tacttgatgc
     2821 tgcaatggat aaaggaacat agtctgattt taaaactgat ttttttcggc tccgtgttag
     2881 tctttgtggc aaatcaagtc gcaaatattg ctaatggcat gagttggcaa gatatttggc
     2941 aaaggatggg acaacaaagc cgcatgacga tcctgctcat gattctggct ggactgctgg
     3001 gtgtcactcc gatgttgttg tatgattggg tgacgatcag agtcttagaa aaacaaggaa
     3061 aaccaaaaat ggaaagaaaa gagtttcttc ttgctgcgtg ggtaacaaac acgatcaaca
     3121 atctcgccgg ttttggtggt gtggtaggag caagtctgcg ggctagtttt tatggaaagc
     3181 agacaaatag aaaaatggtt ttagccactg tatcaaaagt cgcactattt atgctgactg
     3241 gtttgtctgt atggtcgttt cttacattta ttgatgtctt ttttattcag tcagaaagta
     3301 tttttcgaag ctattggatc tggcttttcg gcggtagttt tttagcacca gtcattttct
     3361 tttttgctta tctaaagaga aagacattgt tcttcgagtt ttatccaaaa ggcttgcttg
     3421 gtctgctact ggcatccttt ggacaatgga cgggagccct tgcggtattt ttgatcatcg
     3481 gcaagttgat gagaatagat atttcgatgc tttcgatcta tcctatgttc atgattgcta
     3541 cattgatagg aatgctgacg atggtcccag gtggaatggg gacttttgac gttttgatga
     3601 tcttaggaat gagtcaattg tctgttcggc aagatgtagc agtagtttgg ctattatatt
     3661 atcggttgtt ttattatgtt ctacctttta tgacgggact tctattgttc atcacacata
     3721 caggagtcaa aatcaatcga tttttagata acattccgcg actgtttttc caaaaggttg
     3781 cgcatatcgt gctcgtaagt gctgtctatt ttgcggggat catgatggta cttctatcaa
     3841 caatcactaa tctgtctgca atcagtcggc tatttcaagt attattgccg ttctcattca
     3901 attttttaga tcagacagtg aatctattgg ttggcttttt attactcggc cttgctagag
     3961 cactttcagt aaaagtcaaa aaggcatttc tgcctacgat tgctttacta tcttttggta
     4021 ttttaaatac aatcacacgt acgttatctt ggcagttgat attggtatat gtcttgattc
     4081 tgttagctgt gtggttatcc agaaaagaat tttatcgaac aaaattcgtt tattcttggg
     4141 gagctataat ctttgatgct agcttgttcg gctttttgtt tatcgtttac gcaattgcag
     4201 gctatcacag cggacgcttt ttagtcggtc gaataacagg aaatcatttt attttgtttc
     4261 cttcagatga tgtgtggttc tcagggctta ttggtttggg catctcattg ctagctttag
     4321 tggcgcttta tcaatattta gctgaagata ctacgaagct tggcagtgag tacgaaaaag
     4381 accgttttga ccgtttattg gaaacatacg gaggcacagc tacaagccat cagcttcgct
     4441 tgtcaggtta cagttatttt tattaccaag aagacgacca agatcaagtc gtttttggtt
     4501 atcagatcaa aggaaacaaa tgctttgttt taggagatcc agttggcaat cctaaaatga
     4561 tgcgggcagc aacgaatcag tttatgaaac aagcagattg tttaggctat cagcttgcgt
     4621 tttataagat atcagaaagc tacgtagtga tgcttcatga attgggtttt cacttcacga
     4681 aagtaggtga agcagggatt gttgatctag caacagaaga cttgtctcgg ctgagtcagc
     4741 aacttgaata tcaacgtttg atgaaagaag gataccattt tacttggtac gatcaactgc
     4801 cggaagaatt acatcaagct gtaaatgaag tttcacaaaa atggcttcaa ggtgcacgag
     4861 aaaaatactt ctctgtggga cgatttgata aatcatatgt attatctagc ggtgtcggta
     4921 ttgtccgtaa agatcaaaaa gtcgttggct tcatcaccgc tcagccgatg acgaagaagc
     4981 aggcgggata tgatttgttg cgggtcttac ctaaagaatc tgaggaatta gctgactatc
     5041 tgttagccaa tctgtttatt gtatacgaga aaatgggcta ctcggaagcc aatctaggct
     5101 tagctccatt agctaatgtt ggagaaacag atttttcttt ctttcaagaa aaagtcatgc
     5161 atatcgtata taattatggt aattttttct attccttcca gtctgcttat gaaaagaaat
     5221 taaaatatgt cgatcattgg gaaggacgtt attttgctta taggaaagga agtaatttcg
     5281 gatttgctac catgcagcta ctcagtctga taggaaaaga aaaaggaaat gttccgagtt
     5341 tggcagaaga agtgctcaca gaattataag aatcactaga acttgtttgg attatgaaga
     5401 gaaaattctg tgaaagctgt tgaaacgatg acaaaatcaa ccactaggca gacataagct
     5461 atacttttat ttttccctat gatagaatac ttgcgactta gacttttcga atgggtgaga
     5521 ggatggaatt gattgaaaaa gcgccagcca agataaattt aggactggat gtattatata
     5581 gacggcaaga tgggtatcat gagttggaaa tggtgatgtc gagcgtagac ttggcagatc
     5641 atctattttt tgaagaaata acagaagaca aaatcattgt agaaacaaat aaagcatttt
     5701 taccggtaga tgaacggaac aatgtttatc aagctgcttt attgctaaaa aataaataca
     5761 atatcaaaac cggtgtcaag atctttgtta agaagaatat tccagtagca gctggattag
     5821 gcggaggaag tacagactgt gcagctgctc taagagggat caaccaatta tggaatctgg
     5881 gctgttcgtt agaagaactg gcagaaatcg gtttggaagt cggtacagat gttccttact
     5941 gtgtctacgg aagtacggca tttgtcggtg gcaaagggga agaaatccaa gtattacctt
     6001 ctatgccaca gtgctgggtt gtgttggtca aaccaagaat gagtgtctct acacgtacag
     6061 tgtttagaga agtagatacg caagccatcc aacatcaaga catccacgga cttgctgaag
     6121 ccatccgaga gcaagattat cagcagatga tcaaaaaaat gggaaacagt ttagaagata
     6181 taacgggaaa gcggcatcca gtcatccatc aaatcaaaga gcgtatgatc aagtacggag
     6241 cagatgctgc attgatgagt ggaagcggac caaccgtttt tgccttgtgt caacattatt
     6301 caagagcgca gagagtatat aatgggttaa aaggtttttg tgaggaagtt tatttagtac
     6361 gaaccctgca ataaaatata agaagtaaaa taggtgatga aaaactgagg aatcctaaaa
     6421 tggtattctt cagtttttta ttgtagtgaa atggctagat atgagcagca actcatccat
     6481 tttacgattt aatcaagaat atacatgctt aaatcagaaa ttgatggatt tctattgact
     6541 gtgttttttt attttgatac attagttcac gttaataaat cgtaaaattc cgatttgttc
     6601 attgaaagga gcatataaat gaaaaaatat cttttgacag ctacggtaat gataggtgct
     6661 ttactgtttg cagcatgtgg aaatacgaac aaggaagcag acaaaaaaga ggatttgacg
     6721 atcgttacga ctttttatcc gatgtatgat tttacaaaag agatcgtagg ggatgaagga
     6781 aacgtgaaac ttttgatccc tgctggaacg gagccgcatg atttcgaacc aagtgcaaaa
     6841 gaaagagcag aaatttccga tgcagatgtt ttcgtgtata atagttcaga catggaattt
     6901 tttgtggatt cgttaaagga ttcagtggat agcaaacaga ccttgatgat cgaagcagct
     6961 aaaggaatcg accgtttaga atcgcaagaa gcagacgaac atgaagaatc agaagaaggg
     7021 catggtcatt cacatgaata cgatccacat gtttggctcg atccggtatt agctataaaa
     7081 gaagtcagaa cgattgctga agaattaggc gaaaaatatc cagataaaaa ggaaactttt
     7141 acgaagaatg ccgatgctta tatcaaaaag cttgaagcac tggatcaaaa atactccgat
     7201 gagctaaaag atgcaacaaa ccgcacattt gtcactcagc atgctgcttt tgcttattta
     7261 gcgaatcaat acgacttaaa acaagtagcg atttcgggcg tttcaccaga tcaggaaccc
     7321 acaccaagtc gcttagctga gctaaaagaa tttgtgaaga aaaacaacat caaagtgatt
     7381 tattttgaag aaaatgcctc ttcgaaggta gctgagacat tgtcgaatga aacaggagtg
     7441 aagctagaag tgttgaatcc tttagaaagc ttgactaatg aacaaatcaa agcaggcgaa
     7501 aattatattt ctgtcatgga aaaaaatctg aaagctttaa aagaaagtat caattaagtt
     7561 ttagaatgga ggttgtaagg tgcgttatat cgaaatatct gatttgacct tttactatga
     7621 tgatgaacct gtactagaag atgtctctta tcatgtagat gcgggtgaat ttgtcatttt
     7681 aactggagaa aatggagcag ccaagtcaac cttggtgaaa gcatcgttag gattactgaa
     7741 accttcaaaa ggaatgataa agattgctga gaaaaatcaa gaaggaaaaa aattaagtat
     7801 cggttatatt cctcaacaag ttgcatcttt caatgcaggt tttcctagta cggtgatcga
     7861 attagtccgt tcaggaagat atccaagagg ccgctggttc aagcgtctct ctgaaaaaga
     7921 tcatcaacat gtacagcgtg ctttagaagc tgtagggatg tgggatatgc gaaatcggcg
     7981 aataggtgaa ttgtctggcg gacaaaagca gcgaatcagt ttagcgcgta tttttgctac
     8041 tgaccctgac ttatttgttc ttgatgaacc aacaacaggt atggatgaag aatccagaaa
     8101 tgaattttat cgtcttctcc gacatagtgc gcatgtccat gggaaatcag ttctaatgat
     8161 cacccatgat catgaagata tcaaacagta tgcggacaga cagatccgac tggtgagaaa
     8221 ggaggattct caatggcgtt gctttcatat gaattcatga gaagagcgtt tcttgccgct
     8281 atcttcattg ccggaatcgc cccgatgcta ggtgtgtttt tagtgatcag aagacaatcg
     8341 ctgatggctg atacgctttc tcatgtctct ttagcagggg tagcgttagg attctttttg
     8401 aaccttaatc ctacacttac gaccatgcta gtcgttgtat tagcagctgt gatattggag
     8461 tatctgcgaa cgatctaccg aacctattca gaaatctcca ttgccatttt aatggctggt
     8521 ggtttggcgc tagcgttagt attgatgaat ttgagcggag ggaattcagc aacaagtatc
     8581 caatcctatc tgtttggttc gatcgtgacc attacatggg agcaagtcat ctttttagga
     8641 atcttattcg ttgccatcgg tatattgttc tttttattca aacggccgat gtatgttctg
     8701 acttttgatg aagataccgc ccacgtggat gggctacccg ttcatttgat gtcgatgatg
     8761 ttcaatgtga ttactggtgt ggctattgct gtgatgatcc cgattgccgg tgcacttttg
     8821 atatccgcaa tcatggttct tcccgcagca atcggtatgc gattaggcaa aagcttcaat
     8881 gtcgtgattg ctatcagtgt ttgtgtagga tttctaggga tgctgtcagg actaactagc
     8941 tcgttttatc tagatacacc tccaggagca acaatcacat tagtctttat tgctttgttc
     9001 ctgattgtaa atatcatcaa acgaatattg gttgccttca gaagaaaaaa taactaaatt
     9061 ataaagaaaa gaagccttat aaaaacgaaa aattttttat aggcttcttt ttattttaat
     9121 aaataccgaa ctattttctt attttttcat aaaatgctcg aatttttgaa gaaaaactta
     9181 tataatgtaa aacgataaaa tttcttaaaa ggagttggaa taggtgaaag tacgtcgcag
     9241 tgaacgtcta gttgacatga cacagtattt attgaatcac ccgcatgaat tgatttcctt
     9301 aacaagcttt gccgaacgtt atgaatctgc taaatcttct atcagtgaag atttgggaat
     9361 catcaagaaa acatttaaag aacgagggta tggaaccttg gaaacaatcc cgggtgctgc
     9421 aggtggtgta ttgttcatcc ctgaaatttc atatgaagag gcgaaaaaat atattgaatc
     9481 tctagcagaa cgtctatcag aacaagaccg tctgcttcca ggaggatatg tttatctctc
     9541 tgatttgtta ggagaacctg aactgctgcg tcaagttgga agaatcattg cttctaaata
     9601 ccttggtgaa aaaatcgatg ctgtaatgac tgtagccaca aaaggcgtac ctattgccca
     9661 agctgtgtcc tattatctga atgcgccatt cgttattgta cgccgggact caaaaataac
     9721 agaaggatca acagtaagtg tcaactatgt gtctggatct tctgaaagaa ttgaaaagat
     9781 ggaactgtct aagagaagtt tgaaacgtgg ttcacgtgtc cttgtagtgg atgatttcat
     9841 gaaaggcggc ggaacagtca acggaatgaa gagcttgatt gaagaattcg aatcagaact
     9901 tgttggcatc actgtatttg ccgaatcaaa attcaacggt catcgcgcca tcgatgatta
     9961 tacgtcactg ctttatgtca aagatgtaga tactcaaacg aaaaatatta cggttgtgcc
    10021 aggaaattat ttttcagaat aagaggttgt gaaaaagccg cttagtttcg agtagtaaga
    10081 aaaattttgt gaaaatggct ttccacattt tttcataaat ttggcttaat accgagaaac
    10141 tggcttttga acaccgttta tctagaggtt gtgaaatcag cgtcctgtcc tgagtattag
    10201 ctcaataaac ggaaaaattg ctcctcatat tttgccgttt attgagctaa taccgaaggt
    10261 cagctgattg aaaaacgttt aatgaagagg ttgtaagcct accgtttaac tctaagtcac
    10321 aagaaacgat ttcaaaaaac agcttttcat gttttatgag tagtgtgact tgtgaccgag
    10381 gagttggcag gagagcaccg gttaccaaat agataggccc gttatggtct atctattttt
    10441 ttatccagaa aacgctggga taaaagaaaa tcacttgttt tgttgaaaaa atgggtatac
    10501 tgggataaac tgaaatcagc aaactatatt taggagtgac gcagttggac gcacgttatg
    10561 caattatttt ggcggcaggg aaaggtaccc gcatgaagtc aaaattatat aaagttttac
    10621 atcccgtttc tggtaaacct atggtagaac atatcattaa tcgggtcagt gaaacgaaac
    10681 cagatgaagt gattacgatc gttggacacg gagcagaaca agttaaagca caactaggag
    10741 aacgcagtaa atatgcgtta caagcagaac aattgggaac aggacatgct gttttgcaag
    10801 ctgcttcttt cttggaagga aaaaaaggaa caacgttagt tatcagtggc gatacaccat
    10861 tattgactac tgaaacgctg aacaatcttt ttgaatatca ccaaggaaaa aatgcaagtg
    10921 caacgatctt gacggcacaa gcagaaaacc caacaggata tggacgaatc atccgtgacc
    10981 atattgggat cgtagaaaaa atcgtggaac aaaaagatgc aacaccagaa gaagctcttg
    11041 tacaagaaat caatacagga acttactgtt ttgacaacga agctttgttc gatgcgttaa
    11101 gcaaagttgg aacaaacaat gctcaaggtg aatactatct aacagatatc atcgaaatct
    11161 taaaagaaga aggccataca gttgctgctt atcaaacgga tgattttgaa gaatcaatgg
    11221 gagtcaatga tcggatcgcg ttagctaaag caaatgagat catgagaaag cgaattaacc
    11281 agatgcatat ggtcaatggc gtaagctttg tcgattcagc aacgacatac atcgatgcag
    11341 gcgtagaaat cggtccagac acattgatcg aagcaggtgt acaaatccaa ggaaatacag
    11401 tcattggttc tgattgtgtc atcgggtcac attcaaaaat cgtagacagt aggattgagg
    11461 atcacgtcgt cattgaaaac tcagtcattg aaagcagcca tgtcaagaaa catgcagatg
    11521 tgggacctta tgcacattta cgaccaaaag ctgaaattgg cgaaaacgtc catatcggaa
    11581 actttgtgga agtgaaaaat gctcaaatcg gcaaaggaac taaagtcggt cacttgactt
    11641 atgttggtga tgctacgctg ggagaagaaa tcaatgtcgg atgtggtgtt gtcttcgtca
    11701 actatgatgg aaagaacaaa catcatacga ctgtcggcga ccacagcttt attggttcta
    11761 gcacgaatat catcggacct gtcgaggtag ctaaaaacag ttcgattgct gccgggtcaa
    11821 caattacaga caatattcca gaatatgcgc tggcaattgc gagagcaaga caagtcaata
    11881 aagaaggata tgcaaaaaaa ctcccttatc taaactaaga cttttgcaaa atcacttgat
    11941 ttatttaaaa aatatgacta ctatttatta ggtagaattc tagagttgtg aaaaagaaag
    12001 tggaggtccc catgtcaaaa cattactttg acccaagatt aaaaattttt gcgttaaatt
    12061 ccaatcgccc attagcagag aaaattgcag cagcagtcgg cgtcgagtta ggaaaatcat
    12121 ctgttactca atttagtgat ggagaaattc aagtaaacat tgaagaaagt attcgtggat
    12181 cgcatgtcta tgttattcaa tcaacgagca gcccagtcaa tgataacttg atggagttgc
    12241 tgatcatgat tgacgcactg aaacgagcaa gtgcaaaaac aatcaacgta gtcatgcctt
    12301 attatggcta tgcgcgtcaa gaccgtaaag cacgtgcacg ggaaccaatc acagcgaaat
    12361 tagtagcaaa catgattcaa aaagctggtg caacaagaat gctgactttg gaccttcatg
    12421 cagtccaaat ccaaggtttc tttgatattc cagtcgatca tttgatgggt gcgccactta
    12481 ttgccaatta tttcttagaa aaaggaatca aaggcgatga tgttgtcgtt gtttcaccag
    12541 accatggtgg tgtcacacgg gcacgtaaat tagcggaatt tttgaaatct ccgattgcga
    12601 tcatagacaa acgccgtcca aaagcaaatg ttgcagaagt aatgaatatc attggacaag
    12661 tcaaaggcaa aacttgtgta ctgatcgatg atatgatcga tacagcaggt acgatcacat
    12721 tggctgccaa tgctttgaaa gaagctggtg caactagtgt gtatgcttca tgtacacatc
    12781 cagtattatc cggccctgct ttacaaagaa tagaagattc agctatcgag cgtctagttg
    12841 ttacagactc gatctatctt ccagaagatc gtaaaatcga caaaatcgat gaagtaagtg
    12901 ttggcggact aatgggagat gcaatcaaac gtatccatga aaacaaacca gttagtccat
    12961 tgtttgaaac aaaaaataaa taataaataa actcacaaag gggagtccaa ttgggctgag
    13021 attgaatcta tttctaaacc cttcgtacct gtatcggtta tgcgagcgta ggaattgtga
    13081 ataaacagct gtttgactat tttcgaaagc tgcttcctcc tttgtgactt ttttattcaa
    13141 aggaggaagt ttttttatgg gaaaaaatag agtttgggta gaaggaacga ttgttgcagc
    13201 gttagcgatg gtgttgtcgt tgattccgat ccagatcggc agcagttttt ctatttcatt
    13261 agggcagatt ccattgactc tttttgcgtt aagaagaggg acaaaggcag ggatgctggc
    13321 aggtttcatt tggggcatcc tacattttcc gttgggacaa gtttattatt taagtgtcgt
    13381 tcaagtgttg attgagtacc cgattgccta tacttttgca ggcgcagcag ggctcatggc
    13441 ttcaaatgtc caaaaaggct atgtagacga taatacgaaa caaattagaa aaagcatcgt
    13501 gtttggtgca ttgattggcg cactttccag atatttttgg cattttattg caggagtggt
    13561 tttctgggga gcgtatgcac tatgggggat gaatccttgg ctcttttctt tcgtaatgaa
    13621 tggtgccagc gggttgagta cagctattgt aacgatcatc gttttattag ggatccaaca
    13681 aacgactcct tcgatttttg tgccagataa acgacaggag ctgtaaataa aaaagaagca
    13741 agaaacttga gacagaatca tgtcctagtt tcttgtttcc tttttatttt ttgataagaa
    13801 atagatctac ttcaatggca gataagaaag atcggcatct tctaccaatt gccagtgtcg
    13861 atcggtgaca gagtagatta aagtagcgat gatttcctct tctcgatacc gggtgccttt
    13921 taatttccaa atataatctt caatcacata gattgggata cgatatttac catcgatcaa
    13981 accaacttga tcaaaagaaa aaatgatccc ttcctgcatc aaattacgca tttctttcaa
    14041 taattccaca ctgatttttt caacaggtgt ttcttttttg aaaaaagatt tttttacatt
    14101 gatcttatct gacaagatcg aacttaattt actagcaaaa atcctataaa attcatctaa
    14161 atagcgataa taaatccgat tcagctgatc attaagatcg aagtatacaa aattattctg
    14221 tagtttataa aaaaacggcg aatgaagatg tgttttcata tgaccaaaat ataataattc
    14281 tgaaatttct aaaggtgtca attctttcag catcgttaaa tcagtaaagt cgatccactt
    14341 caattcatcg aatccacgcc tgttacgaga agaggaaaag aaacgctgaa tattttctgt
    14401 accacggatt actttcagcc ctgtatggga ttcatattct ccagcagaag cagatggatc
    14461 aagcaagagc aagttttgag gaaaatgcac gatactgttt gcaaagtcag tagcagtcag
    14521 cccttttgtt aaaacagcat tacttgtggt atcaatatgt acataaactg actcagtcat
    14581 catgttcctc ctcttatttc ctcttaatct cttttatttt acaacatttg cattaaaaaa
    14641 gcttgtttct tttattttaa aattccatta caaaatcacg ttttcattga aaacactctc
    14701 attttcaatt aatttttgtg atttttgtat ttttttattt aaaggaggtt ttatgaattg
    14761 ttttcttatg gaatctacaa agtcgaatac gagactcatg acctttttat gacgtactgg
    14821 tatacttgaa gaaaaaggag agcgcgatgg agataaaaaa agtttacttt tcacagaaaa
    14881 tgtcgctgta ttcgcctatc cagcaagcaa aacaacccct tccatttttt caaagtcatc
    14941 aagaaagtag agacgctttt tggcgttctg caaaaaagca atgtgctttt aaagcagtat
    15001 cgaaaaaata taccgtctat tttactattg ctaaaagtag aggagatgtt atttttaata
    15061 atctgttgat tgaaggtgaa acttttgaat ggaaaaaatt ttttcggtat atggaagctt
    15121 gcgctcgttt ttttattaaa gaaaactgtt gttttttatt tcaatctcct ctatcagaag
    15181 aatggttgcg tatttttcat aaaaacggct atcaaggaac cacacaatta aataaaaagc
    15241 tggtctatca tacagcactt gttttaggta gaggaggagc gcacggtgct tatcagattg
    15301 gtgtgtggca ggcgctgaag gaacatgata tcaactttga gattatcaca ggtacctcgg
    15361 taggtgcgtt gaacggcgca ttgattttac aaggagatat gaaaaaagct gtgaatttat
    15421 ggaaaaagct gtcgacaaga caagtgcttg cgttaccaga aatggctgca gttgaagacc
    15481 ttcgtgaacg cttctatcga gaaagacgcc aaatgacgaa aacagcacta attgaaggcg
    15541 gggtttcttc tgctccattg gaaaagatgg tcaaagacaa tttggatttg tccttactca
    15601 aacataatcc caaaatacgt ttatatacgg tgtctactaa actgccagaa ctagcggaag
    15661 tagtgactga tatccaacag acaaaaacag aagaaatacc agattggata ctcgcctctg
    15721 cttcatttta tcccgctatg gcttatcgaa agatcggaaa aaataaatat gcagacggtg
    15781 ggtatcgcaa caaaatacct attgatattg cgattaacaa aggagcaaca gaggcatttg
    15841 tcgtagatgt gcaagggcca ggcccagcga aaaaaattcg tatgccagac atttttatcc
    15901 attggaaatg tcaaacattg tggacactag gcagctttct gctttttgat agccaaagga
    15961 accaattaaa tttacaatta ggctatctgg aaatgaaaaa acgattaggc tattactatg
    16021 ggaactggta tacatttcat tctgttaaac ctgcaagaac atactggcga ggatttttgt
    16081 cttacttaac aaaagaaatt cagttagaat tatctttttt caaaagcata aaattttggc
    16141 aaaaactgcg taatttatat aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc
    16201 tagaactttt agcaaaaaaa cattttttac tgcccaatga aatctatcaa gtagacatga
    16261 tgattcaaat gatctgccaa caagatataa aaagtatgcc ggatgattta ttggcacaaa
    16321 ttggtcaatt aagtactgaa gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa
    16381 atgagcgcac gaaaactgtt tattttgatt atctgctaca aggaaaacgc aaggatcgtt
    16441 tgcagcatga attgcttaag caaccagttg acacattact gatcctatat ttatattatc
    16501 tcaaggagga acaaccatgg cacaagaatt ttcatatgaa atcgtagaag aaatcgctat
    16561 attgtcagaa aacaacaagg ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg
    16621 cccgccaaaa ttcgatcttc gagattgggc gccagaccat gaaaaaatgg ggaaaggcct
    16681 gacattgacg aacgaagagt ttgaacaact gcaaaaagca atagaaaata tgtaatgagg
    16741 aaggaccctg tcgaatgaaa agtatgactg gttttggcaa agccatccgc gaaacaaaag
    16801 cgtatcagtt agatattgaa ataaaaagtg tcaaccagcg ttttctagat gttcaaatac
    16861 gaagtcctaa aatcctgaac tttgttgaaa acgattttcg tcagcttatt aaacagcagt
    16921 taagccgcgg tcgaatcgaa gtatttgtga atctagctta tattggcaaa aacgaaaaac
    16981 agatcgtcat caactgggaa ttgattgatt cacttatcac tcaattgaca gacggaattc
    17041 aacaacgata cggtagtcaa aatcaagtgt caattagtcg gatggcggaa cgcttcgtgc
    17101 tggacgaaaa cttcgtaacg gtagaagaaa aaccggaaga gagcatggaa gaattggcac
    17161 agttagctct agatacattg aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag
    17221 aaggtcaagc tttagcttta gtgttgaaaa agaatactga agaattcaaa gaagtactaa
    17281 aaaaactcga tagttttgta gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa
    17341 agctggagga atttcttgga gcgaaagtcg atgagcaacg gttgttgacg gagctagcca
    17401 tcttgttgga acgcggagat attcacgagg aactggatcg tctggcgatc catgtacaaa
    17461 aactagatga acttatcgaa gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc
    17521 aagaaatgaa tcgggaaatc aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa
    17581 atcaagtcgt ccagtctaag acgatcttag aaaaaatcag agaacaagta caaaatattg
    17641 aatgacgaaa gaggtagaga taatgaaata ttatgacgta acgttccacg aattaagtgg
    17701 aaaaactgtg gttaaacggg atatcccttc tgaaaaaaac ggctttgacg tatggaaaga
    17761 tgcctgtgca gactataatg agaacgaact gtttattttg atcaacgacg gtgcttacgt
    17821 gacgatgaat cggaaattta ttgttcgaat tgacacagaa gaggtagaag atccgacaga
    17881 aaaagcacgt tctcgaaaag atgagatcat gggtgtagtg aatactttgt ccaatatggg
    17941 cttttagcaa ggatgctgaa caaagaaata caataaataa acagaattgg atttcttcgc
    18001 ttagcttctt tgctctagga atacatttaa aggttgtgac aaaattcgtg tcacaatctt
    18061 tttttatttt gaataagtga tgttcaaaaa ccattttctc ggtattaagc caaatttatg
    18121 caaaaatgtg agaaactatt ttcacaaaat ttttcttcat actcgaaact aatcggcttc
    18181 ttcacgacct cttatacacg gcgttcaaaa gcttgcatct gcgataataa ccccaaccaa
    18241 acgaaaaaca tgagaagctg tttatgtttg gctgttctta ttacttgatg caggacagct
    18301 tttttacgac ctcttggtac agggtagctt ttttaccaat ttctagctat aattaaagat
    18361 aaccttgaaa gttgcagaaa aaaagtgcaa aataaacgat aagtacaaaa agactagttg
    18421 aaaggacgtc gtcatgtcag agcgtggatt attaatcgtg ttgtctggtc catccggtgt
    18481 aggaaaaggc acagtacgaa aagcaatttt tgagagtgag gataatgatt ttcagtattc
    18541 gatctcaatg actacccgta aaatgcgtga aggggaggtc gaaggcgttg attatttctt
    18601 ccgaagcaaa gaagagtttg aatcaatgat cgaagctgga gagatgctgg aatacgcaga
    18661 atatgtcggg aattattatg gaactccttt gtcatatgtc aacaagacat tggatgaagg
    18721 aaaagatgtt ttcttggaga ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc
    18781 cgatggtgta ttcatctttt tgactccgcc agatttagcg gaattaaaat cacggatcgt
    18841 aggccgagga acagatgcag atgaagtaat cgaaaaacga atgaaagttg caaaagaaga
    18901 aatcgaaatg atggctcttt acgactatgc tgttgtaaat gatgaagtgc cgctagcagt
    18961 tgaacgaatc aaaaacatta ttgccagtga acattttcga gtagatcgcg taatcggtaa
    19021 atatattaaa atgttaaagg agatgtagtc ttatgatgtt aaaaccttca attgactcac
    19081 tattagatcg tgtaaattca aaatattctc tagtgatttt agcaagtaaa cgcgcgcatg
    19141 aattggatgc aagtgcacag cctactctag attcttttga ttcagtaaaa agcgtcggtc
    19201 aagctttaga agaaatcgat gcaggaaatg tagtcaatga tccacatcct gaattaaaac
    19261 gtgaacgttt gaagatggaa gaagaggaac gtcaagcaca aaaagagcgt gaacaacacg
    19321 aattggaatc acgtatccgt gaagagcaaa aaatgtaata aagaaaaata gagcgaacat
    19381 ctaaaaagaa aagcaaaccc agctgtttcc ttttcaaaca ttcgccactt attgtctggt
    19441 agaaaaggct gtgtctgaac tttttgacac agttcttttt tttcttatct gctgttaatt
    19501 ttttgttagg atttggcaga agataagaca aatccatcga tctctagtaa aataggcaag
    19561 gaactttttg tcgcttgata agaaaagaca aaaaaaggag gaaacgaacc atgacattaa
    19621 cagctgaagt cattgtggac gtacctacta tgcagaccga tcagccattc acttatttgg
    19681 ttccctctga aatggaaaca gctttgcaag tcgggatgcg agtagaagtc ccttttggta
    19741 atggcaatcg tcatgtccaa ggatttgtta tggctatcca aaaatcagaa gaatcagcta
    19801 atccatcttt gaaggcagtt attcgtttgc tagaccttgc tccggtggtc aatgaagaat
    19861 tactttcatt agctgattat atgaaaaaaa ttacttacgc attcaaaatc acttgtctac
    19921 aaacgatgct tccgagtgtg atgaaagcag aatatgataa attgatctat cctttggcag
    19981 acacaccaga agttcaggta ttgtttgatc agcgagaagt gatttcctgg aaggaagcag
    20041 aagaagaagg cagcctttct caattgattc gctggcgcca agagcaatta gtagatatca
    20101 aatatgaagt ccatacacga aataaagtaa aaacgattcg tttggtccgt tctttgttaa
    20161 ctgaaaagca aatcgaagaa gaatgggcaa aattacggca aaatgccaag aaacaaaaag
    20221 aacttctttt gtgtttatct gaactgagcc aagaagaacc aattgcttat ttcaaaaata
    20281 aaggaatcag tactgctgtc ttgaatcaag gaaaagaaaa gggctggctg gagtttatag
    20341 aatcagaacg ttatcgcgac ccttataaag accgagtatt tgaccaaacg acggcattgg
    20401 aactgaatgc agaacaaaaa aatgctgtcg aacaaatcat cacagcaggt cagcagcaaa
    20461 aagatcaggt ctttttgctg gagggaatca ctggcagcgg gaaaacagaa gtatatcttc
    20521 aggcaatcgc tgatgttttg tcagaaaata aaacggctat tatgttagtg cctgaaatcg
    20581 cattgactcc gcaaatggtc gagcgattca aaggacgctt cggagaatca gttgcggtct
    20641 tacatagcgg actttcccaa ggtgaaaaat atgatgaatg gcgcaagatt gagcgggaag
    20701 aagcgcaagt agtagtaggc gcacgatctg ctatttttgc cccgctgaaa aatatcgggc
    20761 tgatcattat cgatgaagaa catgaatcaa gttacaaaca agatgagaca ccgcgttatc
    20821 atgcgagaga tctagcaatt tggcgcagta aatatcatca ttgcccaata gttctaggaa
    20881 gcgcgacgcc ctctttagaa tcaagagcac gagctcaaaa aggcgtttat cagttattgc
    20941 agttaaacca ccgagccaaa gcagctgcac agcttccagc tattgagatc gtagatatgc
    21001 gtgaagagtt tcagaatcat cggacctcta ctttttctgc taatttgcag gaaaaaatac
    21061 aaaatcgatt agacaaaaaa gaacaaactg tcctactgct caatcgtcga ggatattcgt
    21121 cttttgtgat gtgtagggac tgtggatttg ttcttccatg tcctaattgc gatatctctc
    21181 ttacactgca tatggataca cgttcaatgc gctgccatta ttgcggacat gaagaaccta
    21241 ttcccaatcg ctgtcctaat tgtggcggca ataaaatccg ctactatgga acaggtactc
    21301 aaaaagtaga agaggaactc cgagaacttt atccgcaagc taggattttg cgtatggatg
    21361 tagataccac tcgaagaaaa ggggcacacg aacagatttt acaaaaattc ggtgcaaaag
    21421 aagcagatat attattaggg acacaaatga ttgctaaagg tttggacttt ccagaagtga
    21481 cattagttgg ggtgttgaat gctgatacct ccctgaattt accggatttt cgttctagtg
    21541 agcatacatt ccaattgctt acccaagttt caggacgagc agggcgagcg gaaaaagcag
    21601 gcgaagtcgt gatccaaaca ttcaatcctc aacattacgc catagaattg gctaaaaaac
    21661 aaaattatga gcagttttat cagcaggaaa tgcatgtacg gcatcgtgga ggctatcctc
    21721 cttattactt tactgtgaaa ataaccgcca gccatcctga agaacaagta gcggcaaaga
    21781 aaatctttca gattgccaat cagctaaaag aagtgttaag ccctcaaagc ttgctattag
    21841 ggccaacgcc cagcatgatt ttgagagtga aaaaccgata ttattatcaa ctaatcatca
    21901 aatataaaca tgaaccgaat cttcctcatg ttttggatga aattttgaat ggaagccaga
    21961 aagaacagcg acaaggtctt tttgtggcaa ttgacaacga accattaaac tttatttaag
    22021 aaagggccaa attatgcgct atcctatatt gatccaccct aatgataaat taaaaagaac
    22081 tgctcagcca atcgatgtaa tcacggacga gacaatcgct ttattagaca atttatatga
    22141 gacaatgatt gcgaatgacg gaatagggat cgctgctcct caagtaggac aaaacaagag
    22201 aattgctgtg atcgaagtag atgaaggcga gaaatttgaa ctgattaacc cagaaatcat
    22261 cgaagcaaaa ggtgaaagcc ttgacgtaga aggttgtctc agtattccgc atgtctatgg
    22321 aacggtcaag cgagcagatg aagtgaccgt gcgttattat gatcgagacg gagaagagat
    22381 agaagtgacg gcttttggct atttagcccg tgccttccag catgaaatcg atcacttaga
    22441 cggtatcttg ttcattgaga aaatgataca gcagatcccg gaagaagaac ttgaagaata
    22501 tatggaggaa catcttgatg actaagctag tatttatggg cacacctgct ttttctgtgc
    22561 cgattcttga agggctgata gaatcaggtt acgagatcca ggcagtggta acacagcccg
    22621 accgtccagt tgggcggaaa cgagtaatta cgccgacacc ggtaaaaaaa gcggctctga
    22681 agcacggtat ccgtgtgctg cagccagaga aaatctccgg ttcgccagaa atggaagaaa
    22741 tcattgaatt ggtgcctgat gtgatcgtca ctgcggcatt tggccaattc ttgcctgaaa
    22801 agttattgca agtaccaaaa ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat
    22861 atcggggagg tgcgcctgtt cattatgcca tcatgaatgg ggagaaagaa actggtgtaa
    22921 cgatcatgga aatgatcaaa aaaatggatg caggcggaat ctatgcgcaa gaaagcatac
    22981 caatcactaa acaagatgat gtagggacga tgtttgaaaa gcttagcctt ttaggaagaa
    23041 agctattgct agaaacattg cccaatattt tggatggaca aaaaccagta cctcaagacg
    23101 agtcagaagc aactttttcg ccaaacatca cacgggaaca agaagcaatc gattggcata
    23161 aaactgcaga agaaattgac aaccaagtac gcggaatgcg tccttggcca attgcattca
    23221 cgacctatga gggcatacgc tggaaattat tatcggttga agtattggca gaaactacag
    23281 acaaagcacc tggtacgatc atccggaaag acaaaaaaaa tctatggatt gcttgcggta
    23341 aacagaccgt cttagccatc aatgaattac agccagctgg taaaggaaaa caggccgtac
    23401 atgatttcct taacggaagc ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa
    23461 aaaaatccct aaaaaaatga aaaaatcagt acgatatatg gcattactga cattagagcg
    23521 agtgaacaag ggtggcgcct actcgaatct tttgttgaat gagatgatca aacaaagcga
    23581 gctaaatcaa aaagatgtgg gtttatttac agaattgatc tatggtacga ttagccatca
    23641 gcgactattg gaattttacg ttgcaccatt gatcgctaaa gcaaaaaaag tggatgactg
    23701 ggtcaagatg cttctttatc tttctgtata ccaattagaa tttttagata aagtccctgc
    23761 acatgctatt ttaaacgagg cggtcgaaat cgcaaaagta aaaggaaatc ctgggacagg
    23821 gaaatttgta aatggtgtct tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg
    23881 cattgctgat cctatcgaaa gactttctgt caaaatcagc ctgcctctat ggatgacgaa
    23941 acgactggtg gatcaaatcg gatacgaaga aactgaaaag ctcggtttat cgctatatca
    24001 gcctagccat gctagtgccc gagtagatac acgccgactt tccagagatc aggccatcac
    24061 tgttttgcaa gaagaagaga tcacagctga aaaaagccaa gtcagtccat acggtatagt
    24121 agcgaaaaaa gggcatttag cttctagctc attgttccac gatggcgtca tgacgatcca
    24181 agatgaaagt tcgatgcttg tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt
    24241 agatgcctgt gcagcacccg gaggtaagac gacgcatatt gcttcttttt tagatccgtc
    24301 atgcggtgga gaagtggtcg ctttagatgt acacgaacac aaagtcaaac tcattgaaga
    24361 taatgcagca cgtctaggtg tctcttctgc tgtctacgct caaaagatgg atgctagaga
    24421 agtcagtaaa cagttcgaag cagagcagtt tgatcggatt ttagtagacg cgccttgttc
    24481 aggtcttggg ctgcttcggc gaaaaccaga tattcgttat aaaaaacagc cggatgaatt
    24541 agcgaattta ccaaaaatcc aattggaaat tttagaaagc tgcgcaccaa ctttaaagtc
    24601 ttccggtatt ctgacttata gcacttgtac gatcttaaaa gaagaaaacc aagacgttgt
    24661 ttctgctttc ttggaaagac accctgattt tgagaggatt gatgttttga ccaatcctat
    24721 gctacaatca tcaatcgaag agaaaatgtt gactttgtat ccgcatcagt tttacacaga
    24781 tggatttttc atttgttgtt tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata
    24841 tcaatcagat gtaggaagac gacgtaacac aaatcaagac tatgccagtg tatttaccag
    24901 ccgttcaggt ctcaagcttg ctgtattagc agacgggatg ggtggtcatc gtgccggaga
    24961 cattgccagt cagatggcgg taacgaatct aggcgaagct tgggaaaaac aagaagctgt
    25021 tgacgatgaa aaaatcgccc aatggttcat tcaatcgatt caagaagaaa atatgctgat
    25081 ttatcagcgt ggacaagaac agccggaata taacggtatg ggaacaacaa tcgtagcggc
    25141 cgctctgtca gatgactggt tcacgattgc caatgttggg gatagcagag cttatttgat
    25201 tcgtgaagat aaaatcatgc aattgacgga ggatcattct ttagtcaatg aactagtcaa
    25261 atcaggagaa atcagcgaag aaatggcagt gaaccatcca aggaaaaata ttttaacacg
    25321 ttctgtcggc atgcctggaa cagtcgaagt agatgtttct actcatcttt ggcaactaaa
    25381 agatcgactg ctgttatgtt cagatggctt gacgaacatg ctgtctgaag aagaaatagg
    25441 aacgatcatc aatcaagaag gcccattgtc tcataaagta actgagctga ttgatcgtgc
    25501 caatgaagca ggcggagcgg ataatatcac cgttcttctt atcgaatata aggaggacag
    25561 tgtatgatcg agttgggcaa aaaattaaat ggccgttatc aaatcactgg aaatatcggt
    25621 agcggtggta tggcaaatgt atttttggcg cacgatttaa tcttggatcg tgatgtagca
    25681 gttaaagtct tacgatttga ctttcaaaat gaccagactg cgattcgacg atttcagaga
    25741 gaagctttag cagcaacaga actcgttcat ccaaacattg ttagtgttta cgatgttggt
    25801 gaagaagaca acatgcagta tctggtgatg gaatacgtca aaggaatgga cttgaaacga
    25861 tatatccaaa cacattatcc tgttccttat gaaacagcag taaatattat gcagcagatc
    25921 ctttctgcta tttctttagc tcataaccat caaatcatcc accgagattt gaaaccccaa
    25981 aacgtactga tcgataatga aggtgttgta aaaatcacgg atttcgggat tgctatcgct
    26041 ttatctgaaa catcgatcac gcagacgaat acgatgctag gatctgttca ttatctgtcc
    26101 cctgagcagg ccagaggaag tatggcaacg aagcagtcag atatttatgc tttagggatc
    26161 atcctttatg agatgttgac gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt
    26221 gctttaaaac attttcaaga tgatcttcca tctataaaag cacttgatcc aaatgtgcca
    26281 caagcactgg aaaacgtgat tttacgggca acggctaaag aacctgcaga ccgttataag
    26341 tcagccgagg aaatgtctga tgatctttct acagtattat caccagctcg tgcaaacgaa
    26401 gaaaaatggc agcctcatgt gatggataat gaaacaaaag ttatcacacc ccttacggaa
    26461 gatgcaccaa tgcctgattc attcaaatct atgcctctgc caaaagacaa gccagaaaac
    26521 gaaaccttac cagaggagga agaagttctg cctgatccta aaaagaaagg acagaaaaaa
    26581 tggtggctga tcctattagc tattcttgct gttttaggaa tcggaacagc tgtattcttt
    26641 gcttctggcg ggcgaggaga agttgttgtt cctgatgttt ctgatctttc tgaagcttct
    26701 gctagagaaa cactcgcaaa agcgaaatta gaagtagcaa caaaaacaga agaaattgca
    26761 gatgacaaaa tcgaagaagg taatgtagtg aagacagacc cggcagcggg aacgacagtc
    26821 aagcaaaaac gcgaagtcac tctgtatatt agtagcggca cgaaaaaaat caaattagat
    26881 gattacagtg gtaaaagtta tgaagatgcc gaagcaagtt tgaaagaatt agggttttct
    26941 gctgacttga ttcaaaaaga agaggaattc agttcagagg tagaaagcgg aatgatcatt
    27001 tcgcaatctc catcagaaga tacagaagtc gatcctaaaa cagacacgat tactttcaca
    27061 gtcagcaaag gaccagaaac agtgactttg agtagtcatg taggagaaaa ctacgaaact
    27121 gttcgtaatt ggcttgttac acaaggattc aatatcaata atattagtgc tacttatgac
    27181 tatagcgaca ctgtaccatc aggttcgatc atcagtcagg atccagcctc gggtgaagtt
    27241 gtagcagaag aaacttacat caattttgtc gtaagccaag gaacagagcc taaattgaat
    27301 gatatctccg gctatacaaa aagcgaagca caaagttatt tagctagtgt cggtgcagaa
    27361 tatatcgggc atgagacttc cgaatattca aacacggtgg ataaagataa agtgattcgt
    27421 acagtaccag gggcaggaac gacaattacc aaaggaatgg ttgtgaacgt aatttactct
    27481 aaaggaccag atccagcgtc ttctagttct gaaaaagact cttcagacag ttctagcagc
    27541 tcaagtagct ccagtagcac aagcaactct tcaaaagaag aatcttccag ctccacaagt
    27601 tcatcagcta gcacctcaga aagttcaaaa taagtgctga ataaaatata tcagaaggga
    27661 atgcgacatt gatttgttgc attctctttt ttgtgtttct atctcttttg atcaattttc
    27721 aagccatata ctttgagaag tttgcatgga ataacaccta ctttcctctt cgtatggtaa
    27781 aataaacaaa aacggaggtg gcacttatca aaggacaaat cagaaaagca atcagcggtt
    27841 tttactatgt atacgcaaac ggagagacct atcaaacaag aggaagagga aatttccgga
    27901 atcggaaaat cacgccgctt gtcggagatc aggtgatctt tgagagtgaa aatcaaacag
    27961 acggctatct tctggaaatc ttgccgagaa aaaatcaatt agttcgccca ccagtcacaa
    28021 atgttgatct tggaatcatt gtcacaagta tcgtagagcc tgcattttct tataatctac
    28081 ttgaccgctt tctagttacc ttggaatacg agtcgatcga accgattatt tttttgacaa
    28141 agaccgactt gtcagacgac ccagaaaaaa tcgaagagat caagcaaacc tatactaaaa
    28201 taggctatcc ggtgattgta cccaatcata cgggtgatac gacagaactg atccgttact
    28261 tcccagaacg gctatcggtc tttatgggtc agtctggagc aggaaaatcg acgctactga
    28321 atcaaatatc accggaactg aatttagcaa caggagagat ttctgatgca cttggaagag
    28381 gacggcatac aacacgtcat gtggaactga ttcctatcta tggcgggtta gtagcagata
    28441 caccaggatt tagctcgatt gactttttga caattgaagc aggagaactg ccgagacaat
    28501 ttcctgaatt tgtagaagct tctgtccatt gcaaatttag agagtgcatg catgctaaag
    28561 aacctggctg cgaagtaaaa catagagtag aaacaggtgg tatcgcacag acacgttacg
    28621 acaattattt gcaattctta caagaaatcg aaaaaagaaa accaatgtat gcaaaaaaag
    28681 aaggtaaacg aaaataggag gaaacaagaa tgaagatagc accatccatt ttgagtgcag
    28741 attttgccaa tttacaaaga gacattgagt tagtggaaaa aggaggcgca gattacatcc
    28801 acgtagatgt aatggatggc cgatttgtcc caaatatcac attagggcca aatattgttc
    28861 aggcaatccg tcctgttact aaattaccgt tagacgtcca tctgatgatc gttgatccag
    28921 aaaactacat tccagcattt gcaaaagctg gagcagatat cattactgtc cacgtcgaag
    28981 caactcctca tattcatcgg gcacttcaaa tgacaaaaag cttaggagtt aaagctggag
    29041 tagttatcaa tccaggaaca ccagtctcca tgatcaaaca tgttttgcct ttggctgatc
    29101 aagtactagt catgactgtc aatcctgggt tcggcggaca aagctttatt gaagaaacag
    29161 tagaaaaaat tacggaactc gctgatctac gtagagagaa caattggact tatgcaatcg
    29221 aagtagatgg cggaatcgtt ccagaaacag caaaaatctg taaagatgca ggggcggatg
    29281 tcttcgttgc aggatcatat atctatggag ccgaaaatcc agtcggacaa atcgaacgat
    29341 taaaagaggc attagactaa atgaaaattt tattagctgc tggaggtaat ccgcaaaact
    29401 ggccgcagtc tctgctgtcc aaatacgatg cctatgtcgg gatagaccgt ggtgcgctgt
    29461 acctgcttca agcaggctac ccagtagatc tggcagtggg tgattttgat tcattgtcga
    29521 acgaagagca gaaggaagta tttgcaaaag ccaaagagta tacccgttcc cctgcagaaa
    29581 aagacgatac agatacacag ttaggtatct taaaaacatt tgaacgttac ccgcaagcgg
    29641 aagtcacttt gattggagct acgggcggaa gattggatca tcttttagcc aatctctggt
    29701 taggtatgga acctcgcttc aaaccctttg cccatcagct tattatcaga gattgtcaaa
    29761 attatttgtc ttattatttg cctggagagc atatagtaag acagatacca gatatgaagt
    29821 atttagctta ttgctgcttg actcccgtct cgaatcttac attaaaaggc agcaagtatg
    29881 tattggaaaa taaagaagta gaatatccga catcttatgc aagtaacgaa tttctttcag
    29941 ataccgcaga attttctttt tcagatggta tgattgcagt tattcaaagc aaagattgat
    30001 ccataaacaa ataaaaaaat ttgtctttca ggctaaaaat gacagaatct attatttgct
    30061 aaaaaaaagc ttaagaggag caacttacct tttgtttcac ggcatttagt gataaaattg
    30121 aacacaagag tctaattaat tcgaggtgtc tttgtgaaga agttcaatcc taataaaaat
    30181 attattatta ccttggttat tgttatcatc gtggtgacga tactaagtgt gaccgctgcg
    30241 cagcgagcga atgaaggaaa gcccaatttc ttccaatcca tcgttaatga cagtgttgca
    30301 atggttgata aaacaatctc tgccccagta cggtgggtac gaaatggagt agattctgtc
    30361 cataatcttt tttcaactta tgccgaaaat gagcggctaa aagaaaaaat cgatagctat
    30421 gatgacgttt cacagcaaaa caagaattac gagagagaaa tcgaagcgtt gaaaaaagaa
    30481 cttgagttga atcagacgct gactagttac gaaaaagtaa cggcaaatgt cattactcgt
    30541 tcgcctgaca gttggcagga catgctgatc gtagacaagg gttctaaaga cggtatcgaa
    30601 gcaaatatgg cagtcatgtc gcaaaaagga ttgattggcc gagtgatcga ggtcaatacg
    30661 gcttcgtcta aaatcgaatt actgtcatcc tctaatgaaa gctccaatca ttttccagta
    30721 cgggtatctt cggctaatgg cgaagcgttt ggtttgctta aaaactatga tgaaaagctc
    30781 catgccttag tggtgaccca attaactggt gatacggata tcaaagaagg ggatgttgtc
    30841 cagacatccg gtcttggagg gaattctcca gctaacttgc cgatcggtac ggttattaaa
    30901 acgaaaccag atagttatgg gctggatcgg gaagtttatg tgaaacctta tgcagaaatg
    30961 tatgacgtgt cagttgtgac gattgtccaa cggctagcgg aggattagtc catgttgaag
    31021 aaagaaacaa tgaagtacta tctgccaatc gttttgttct ttttgatgtt gatagatggt
    31081 catttaacaa gaatgctagg ggagtggtcg aaaggcacct atatgtcaaa tgcccacttt
    31141 ctgatattgg cattattatg ttgcagtatg gcgtttgaaa aacgttattt actgattacc
    31201 acgattgttc tcggggctat ctatgacgct tactatattg gcgttatcgg tatctatgca
    31261 gtagctctcc ctttaattgt atggttgatg tatgtaatga aagacgttat ccatgtcaac
    31321 atctttactg aatttttcag tatgatcatc tttgtcacgg gttatgaatt gtttacgatg
    31381 gtggtccagt tgatttttaa attagcagta gtaaataaca cgtattttat tacaaggttt
    31441 ttaggaccta cactgctgtt gaacatgatt atatttgtat tattcatttt tccctttaag
    31501 aaattattca gtgatgaata atttcttttt ttatttttag gtcgaaagcg aaaaagaatc
    31561 gttgttatct taagaaatgc tagacttaac gcggcttttt catgatcatt ttctgaaacg
    31621 aacagttttg tcatttttaa aagttgtttt gaaacatttt tgtaacataa ctattatatc
    31681 gttgacacac tgctatggta caatttttat atcgttaaga aagacttaaa tatatttaga
    31741 tatttattaa attaagccag tcggaggaat gaagagtgaa aaagagttta atatcagcag
    31801 taatggtatg ttcaatgacc ttgactgccg tagcatcacc gatcgctgcc gcggcggacg
    31861 attttgattc tcagatacaa cagcaagatc aaaagattgc agacttgaaa aatcaacaag
    31921 cagatgcaca atcacaaatc gatgccttag aaagccaagt ttctgaaatc aacacacaag
    31981 cacaagacct attagcaaaa caagatacat tgcgtcaaga atctgcacaa ttagtaaaag
    32041 acattgcaga tttacaagaa cgtattgaaa aacgtgaaga tacaatccaa aaacaagcac
    32101 gcgaagcaca agtaagcaac acaagctcaa actacatcga tgctgtattg aatgctgatt
    32161 cattagcaga tgctattgga cgcgttcaag caatgacaac gatggtaaaa gcaaacaacg
    32221 acttgatgga acaacaaaaa caagataaaa aagctgtaga agacaaaaaa gctgaaaacg
    32281 atgctaaact aaaagaatta gctgaaaacc aagctgcttt ggaatctcaa aaaggcgatc
    32341 ttttatctaa acaagctgat ttgaacgtac tgaaaacttc attagctgct gaacaagcaa
    32401 ctgctgaaga caagaaagct gacttgaacc gtcaaaaagc agaagctgaa gcagagcaag
    32461 ctcgtattcg tgaacaacaa cgtctagcag aacaagctcg tcaacaagct gcacaagaaa
    32521 aagctgaaaa agaagcacgc gaacaagcag aggctgaagc acaagctact caagcttcat
    32581 ctactgcaca aagctcagca acagaagaat catcagctac acaatcttca atgactgaag
    32641 aatcatcatc agctacacaa tcttcagcaa cagaagaatc aacaacacct gaatcttcaa
    32701 cagaagaatc aacagcgcct gaatcttcag caacagaaga atcaacaaca gcgcctgaat
    32761 cttcagcaac agaagaatca acaacagtgc ctgaatcttc agcaacagaa gaatcaacaa
    32821 cagtgcctga atcttcaaca acagaagaat caacaacacc tgcgcctaca acaccatcaa
    32881 cagatcaaag tgttgataca ggaaacggca caggaagtag tactccggct ccaacgccaa
    32941 caccaacacc tgaacaacca aaacctgtaa cacctgctcc agctccttct ggaagtgtga
    33001 atggtgcagc aatcgtagca gaagcttaca aatatattgg tactccatat gtttggggcg
    33061 gtaaagatcc aagtggcttt gactgctcag ggttcacacg ctatgtttac atgcaagtaa
    33121 ctggtcgtga catcggtggt tggacagttc ctcaagaatc agctggaaca aaaatttcag
    33181 tttctcaagc aaaagctgga gacttgttat tctgggggtc acaaggcgga acttaccacg
    33241 tagcgattgc tttaggcgga ggacaatata tccatgctcc tcaaccaggc gaaagcgtaa
    33301 aagttggttc agttcaatgg tttgcccctg actttgctgt cagcatgtaa gaatga
//
LOCUS       DAZAJZ010000023        33166 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000023 DAZAJZ010000000
VERSION     DAZAJZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.023"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACG86D_001048"
     CDS             complement(29..1261)
                     /locus_tag="ACG86D_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN3140208.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACG86D_001049"
     CDS             1516..2085
                     /locus_tag="ACG86D_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3140209.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACG86D_001050"
     CDS             complement(2263..2703)
                     /locus_tag="ACG86D_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN3140210.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACG86D_001051"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACG86D_001051"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN3140211.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACG86D_001052"
     CDS             3657..4580
                     /locus_tag="ACG86D_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN3140212.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACG86D_001053"
     CDS             complement(4805..5044)
                     /locus_tag="ACG86D_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140213.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACG86D_001054"
     CDS             complement(5049..5468)
                     /locus_tag="ACG86D_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN3140214.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG86D_001055"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG86D_001055"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN3140215.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="ACG86D_001056"
     CDS             complement(7040..7675)
                     /locus_tag="ACG86D_001056"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3140216.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="ACG86D_001057"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="ACG86D_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="ACG86D_001058"
     CDS             complement(8017..8652)
                     /locus_tag="ACG86D_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3140217.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="ACG86D_001059"
     CDS             9164..10306
                     /locus_tag="ACG86D_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3140218.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="ACG86D_001060"
     CDS             10299..10505
                     /locus_tag="ACG86D_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN3140219.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="ACG86D_001061"
     CDS             10584..11777
                     /locus_tag="ACG86D_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3140220.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="ACG86D_001062"
     CDS             11982..13121
                     /locus_tag="ACG86D_001062"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN3140221.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="ACG86D_001063"
     CDS             13114..13914
                     /locus_tag="ACG86D_001063"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3140222.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="ACG86D_001064"
     CDS             13914..14741
                     /locus_tag="ACG86D_001064"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3140223.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14746..15453
                     /locus_tag="ACG86D_001065"
     CDS             14746..15453
                     /locus_tag="ACG86D_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3140224.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="ACG86D_001066"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="ACG86D_001066"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN3140225.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="ACG86D_001067"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="ACG86D_001067"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN3140226.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="ACG86D_001068"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="ACG86D_001068"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN3140227.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="ACG86D_001069"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="ACG86D_001069"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN3140228.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="ACG86D_001070"
     CDS             19038..21071
                     /locus_tag="ACG86D_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140229.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="ACG86D_001071"
     CDS             21115..22395
                     /locus_tag="ACG86D_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN3140230.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="ACG86D_001072"
     CDS             22605..23411
                     /locus_tag="ACG86D_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140231.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="ACG86D_001073"
     CDS             23423..24643
                     /locus_tag="ACG86D_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140232.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="ACG86D_001074"
     CDS             24633..26219
                     /locus_tag="ACG86D_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3140233.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="ACG86D_001075"
     CDS             26258..28396
                     /locus_tag="ACG86D_001075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3140234.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="ACG86D_001076"
     CDS             complement(28764..29747)
                     /locus_tag="ACG86D_001076"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3140235.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="ACG86D_001077"
     CDS             30125..31516
                     /locus_tag="ACG86D_001077"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN3140236.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="ACG86D_001078"
     CDS             31532..32572
                     /locus_tag="ACG86D_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3140237.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..>33166
                     /locus_tag="ACG86D_001079"
     CDS             32591..>33166
                     /locus_tag="ACG86D_001079"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3140238.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDIS"
BASE COUNT        10690 a         5742 c         6550 g        10184 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctca
//
LOCUS       DAZAJZ010000024        32211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000024 DAZAJZ010000000
VERSION     DAZAJZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.024"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1021)
                     /locus_tag="ACG86D_001080"
     CDS             complement(146..1021)
                     /locus_tag="ACG86D_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3140239.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1130..2899)
                     /gene="aspS"
                     /locus_tag="ACG86D_001081"
     CDS             complement(1130..2899)
                     /gene="aspS"
                     /locus_tag="ACG86D_001081"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN3140240.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2914..4206)
                     /gene="hisS"
                     /locus_tag="ACG86D_001082"
     CDS             complement(2914..4206)
                     /gene="hisS"
                     /locus_tag="ACG86D_001082"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN3140241.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4600..5547
                     /locus_tag="ACG86D_001083"
     CDS             4600..5547
                     /locus_tag="ACG86D_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3140242.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5636..6895)
                     /locus_tag="ACG86D_001084"
     CDS             complement(5636..6895)
                     /locus_tag="ACG86D_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN3140243.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7066..7512)
                     /gene="dtd"
                     /locus_tag="ACG86D_001085"
     CDS             complement(7066..7512)
                     /gene="dtd"
                     /locus_tag="ACG86D_001085"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN3140244.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7523..9736)
                     /locus_tag="ACG86D_001086"
     CDS             complement(7523..9736)
                     /locus_tag="ACG86D_001086"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN3140245.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9969..10733)
                     /locus_tag="ACG86D_001087"
     CDS             complement(9969..10733)
                     /locus_tag="ACG86D_001087"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN3140246.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10734..11681)
                     /gene="prmA"
                     /locus_tag="ACG86D_001088"
     CDS             complement(10734..11681)
                     /gene="prmA"
                     /locus_tag="ACG86D_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN3140247.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11694..12182)
                     /locus_tag="ACG86D_001089"
     CDS             complement(11694..12182)
                     /locus_tag="ACG86D_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN3140248.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12148..12819)
                     /locus_tag="ACG86D_001090"
     CDS             complement(12148..12819)
                     /locus_tag="ACG86D_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN3140249.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12957..14243
                     /locus_tag="ACG86D_001091"
     CDS             12957..14243
                     /locus_tag="ACG86D_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN3140250.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14315..14509
                     /gene="ssrS"
                     /locus_tag="ACG86D_001092"
     ncRNA           14315..14509
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG86D_001092"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14587..14871
                     /locus_tag="ACG86D_001093"
     CDS             14587..14871
                     /locus_tag="ACG86D_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140251.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14944..15423
                     /locus_tag="ACG86D_001094"
     CDS             14944..15423
                     /locus_tag="ACG86D_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3140252.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15646..15789
                     /locus_tag="ACG86D_001095"
     CDS             15646..15789
                     /locus_tag="ACG86D_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN3140253.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16134..16262
                     /locus_tag="ACG86D_001096"
     CDS             16134..16262
                     /locus_tag="ACG86D_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140254.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            16367..17950
                     /gene="abc-f"
                     /locus_tag="ACG86D_001097"
     CDS             16367..17950
                     /gene="abc-f"
                     /locus_tag="ACG86D_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN3140255.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18387..19571)
                     /locus_tag="ACG86D_001098"
     CDS             complement(18387..19571)
                     /locus_tag="ACG86D_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN3140256.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19596..20603)
                     /locus_tag="ACG86D_001099"
     CDS             complement(19596..20603)
                     /locus_tag="ACG86D_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3140257.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20775..21134)
                     /gene="comGG"
                     /locus_tag="ACG86D_001100"
     CDS             complement(20775..21134)
                     /gene="comGG"
                     /locus_tag="ACG86D_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN3140258.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21106..21531)
                     /gene="comGF"
                     /locus_tag="ACG86D_001101"
     CDS             complement(21106..21531)
                     /gene="comGF"
                     /locus_tag="ACG86D_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN3140259.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21494..21841)
                     /locus_tag="ACG86D_001102"
     CDS             complement(21494..21841)
                     /locus_tag="ACG86D_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140260.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(21813..22283)
                     /gene="comGD"
                     /locus_tag="ACG86D_001103"
     CDS             complement(21813..22283)
                     /gene="comGD"
                     /locus_tag="ACG86D_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN3140261.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22258..22536)
                     /gene="comGC"
                     /locus_tag="ACG86D_001104"
     CDS             complement(22258..22536)
                     /gene="comGC"
                     /locus_tag="ACG86D_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN3140262.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22533..23582)
                     /gene="comGB"
                     /locus_tag="ACG86D_001105"
     CDS             complement(22533..23582)
                     /gene="comGB"
                     /locus_tag="ACG86D_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN3140263.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23539..24468)
                     /gene="comGA"
                     /locus_tag="ACG86D_001106"
     CDS             complement(23539..24468)
                     /gene="comGA"
                     /locus_tag="ACG86D_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN3140264.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24600..26354
                     /gene="ade"
                     /locus_tag="ACG86D_001107"
     CDS             24600..26354
                     /gene="ade"
                     /locus_tag="ACG86D_001107"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN3140265.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26452..27114)
                     /gene="trmB"
                     /locus_tag="ACG86D_001108"
     CDS             complement(26452..27114)
                     /gene="trmB"
                     /locus_tag="ACG86D_001108"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN3140266.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27184..27966)
                     /locus_tag="ACG86D_001109"
     CDS             complement(27184..27966)
                     /locus_tag="ACG86D_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN3140267.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28170..29381)
                     /locus_tag="ACG86D_001110"
     CDS             complement(28170..29381)
                     /locus_tag="ACG86D_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140268.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29374..30111)
                     /locus_tag="ACG86D_001111"
     CDS             complement(29374..30111)
                     /locus_tag="ACG86D_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140269.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30249..30683
                     /locus_tag="ACG86D_001112"
     CDS             30249..30683
                     /locus_tag="ACG86D_001112"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN3140270.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30676..31020
                     /locus_tag="ACG86D_001113"
     CDS             30676..31020
                     /locus_tag="ACG86D_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN3140271.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31200..32210
                     /locus_tag="ACG86D_001114"
     CDS             31200..32210
                     /locus_tag="ACG86D_001114"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3140272.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         9637 a         6747 c         5743 g        10084 t
ORIGIN      
        1 aaataaacat gctttttttc aactttaaga aaatttcaaa taaaaaagca gtttttcttt
       61 gctgattcta ctatggtaga aaaaaactgc agacaaacga acgtctgtct acagcagtat
      121 ttcatgaatt tttgtcaaac tcgatctaaa tgccatcctc atagaaacga ccaagtatct
      181 ggacgttttc tgcaatactt acaaaagctt gtggatcaga agctttcatt gcttctctaa
      241 gtagaggcag ttcgtatcgt gtaacgatcg tcatcaatac tgtctgctta tcatgcttat
      301 aagcaccttc tgcttcattg atgatcgtga tccctctacg catacagttt tggatttcat
      361 ttactacttt ttctggatgt ttcgttacga tcattacctg catcttcttt tgtttagtat
      421 aaacgacatc agttactttt ccactgacga aaatagacaa cgtactgtaa aacatatatt
      481 gccagccaaa tagaattccg gcaacaaaaa cgatcaatgc attgaagtag atcgaaatag
      541 aaccaatcga ctttcctgtt tttttacgaa tcgtgatact cacgatatct agaccgcccg
      601 atgataggcc gtttctcaac gctagcccaa tacctagtcc catcacagcc cccccaaaca
      661 acgcacagat gattggatcg gtagatagga ctgtttccgg taaaatatgg atacaaaggg
      721 aggtcaaagt gactgtgata aaagtgaaaa ttgtaaactt cttcccgatt tttaaccacg
      781 ccaatacaaa taaaggtata ttcagtaaat acaaagttgt tgataccggt acttcgaatc
      841 ctatcagtga ttttgacaaa gtcgtcaaaa tctgagctag accagtaata ccacttgaat
      901 aaatatgtcc tggctgataa aagaaattca atgcgactga tgcgatcaac gcataaacga
      961 tagataccga aagcttggtc gtgtaatcat gataagccca attttcgaat gtttttttca
     1021 tggaaaagcc ccgctttata tgttatggct tcattataaa gtgtttttca attaaatcaa
     1081 ggttgtcaaa agaagcgtat tcgacaaatc ctttttacct ttttatcggc tattcttcta
     1141 tattcgttac atcaatattc aattcgaaca attgtaaagg cgaaaccata cttggtgcat
     1201 cactcatcac atctgctgct tttccgtttt taggaaaggc aatgacttca cgaatattat
     1261 cttctccggc taataacatt gcaaaacggt ccaatcctaa tgcaataccg ccatgtggag
     1321 ggaatccgta atctaaagct gttaacaaga aaccaaattg ttcttccatt tgttcttttg
     1381 taaagcctag agtttctagt actttttctt gaagctcacg agtatgaata cggatagaac
     1441 ctccgcctag ttcataaccg ttcaagacga tatcataagc ttctgcatag acttttgcag
     1501 ggtcttcagc caataacggg atatcttctt cttttggcat cgtgaatgga tggtgagcag
     1561 agacatatcg accttcttct tctgaaaatt caaattgtgg ccaatcagtt acccataaga
     1621 aattgaattt cgattcatca attagtccta attcttttcc taaacgtgtc cggatagcac
     1681 ctaatgcagc agcgacgatt tcacttttat ctgcgccaaa catcagcaag tcacctggtt
     1741 ttgcatctgt tgctttaatg attgcttctg ttgcttctcc catgaattta gcaattggac
     1801 ctttcaatcc gtcttcttct actttcagcc atgcaagccc cttagctccg aattgtccta
     1861 catattgacc taactgatcc atgtcttttc gtgagtagcg atctgcagcg cctttagcat
     1921 tcaatgcttt cacgacaccg ccgttttcaa gagccatttg gaagactttg aattctactt
     1981 ctttgactgt atcgcttaga tcgatcaatt ccatatcaaa acgtgtatcc ggtttgtcgc
     2041 tgccgtagcg agccattgct tcatcatagg tcatacgagg aaatggcaat gtgacttcga
     2101 taccgcgaac ttctttcatt accttcgcaa tcaatccctc tgtgtaagtt tggatttctt
     2161 ctgctgttaa gaacgttgtt tcaatatcta cttgcgtaaa ttcaggctga cggtctccac
     2221 gtaaatcttc atcgcggaaa caacgaacga tttgataata acggtcgaat ccagcattca
     2281 tcagcaattg tttaaacaat tgaggagatt gcggcaacgc atagaaatgt ccagcatgga
     2341 cacgtgaagg aactaggtag tctcttgcgc cttctggtgt agatttccct aggtaaggtg
     2401 tttcgatatc gagaaagtca ttatcatcta aataatgacg aatcgctttt gttgtcgcat
     2461 ttcttaattt gatgttttta gtcatttttg gacgacgcag atccaaataa cgatatttca
     2521 aacgtatttc gtcactgatt gcttgatcat cttcaatttg aaatggcggg gtttttgctt
     2581 gattcaaaat cacgatttca cttgcaatca cttcgaattc accagtggcc attttaggat
     2641 ttactgcatt ttctccgcgt cttctaactt ccccagtcac ttcgatcacg tattcactgc
     2701 gacatttatc cgcgatttcc catgcttctt tgttgatttc aggattaaag acgatttgaa
     2761 cgattccttc acgatcacgt aggtcgataa agattactcc cccaaggtct cgtctttttt
     2821 gtacccatcc ttttaatgta actgtttgtt ctaattgttg ttcggaaact aatccgcaat
     2881 agatcgttct ttgtgccatt tatttttttc ctcctactcg aataaagtca tttcatcata
     2941 gacttcatca aaatgttcat atatctcttc taatggaaaa cttttttctt ttcggcttgc
     3001 cattgctttg acattaatcg tttgactagc aagctcatct tcaccgatca ccaatactaa
     3061 tttggcattc gctttatctg ctgtcttaaa ttgcgctttt gctttacggt tcatgaagtc
     3121 gcggtcagct gaaaaaccaa agtttcgaat cgcttggacc actttcaatg cttcgatatt
     3181 tgtttcgtct ccgagtgcga ccacgtaagc atccaactca ttaaatgcag ggataactac
     3241 ttcctctgcc tccatagtca agagtaatcg ctcgataccc attgcaaatc cgaagccagg
     3301 tgttgaagga ccacctagtt cttccactag attgtcataa cgtccgcctg cgcaaatcgt
     3361 tgcttgtgcc cccatttttg gtgcgtcact catgatttca aaaatcgtat gtgtgtaata
     3421 atctagtccg cgaaccatat tactgtcgat ttcataagga acagccaatg catccagcat
     3481 ggaaacaacg gtatcaaaat gtttttgagc aggctcgctt agataatcta agatagatgg
     3541 agcttctgcc acaaattttt gatctcgttt gtctttactg tccaatacgc gtaatggatt
     3601 ttcatgaagg cggcgtttag agtcttcact cagctctgat tcaaaaggta aaagatagtc
     3661 gatcaatgct tgacgatagg cttgacgcgt ttctttatca cctaatgaat tgatgactag
     3721 ccggatttgc gtgatcccca actgtttgaa gaaatcaagt gccatcacca tgctctccac
     3781 atcagttgca gggttttctg atccaaatgc ttctactccg atttgatgga attgacgtaa
     3841 ccgtcctttt tgagggcgtt cgtagcgaaa cattggtccc atataaaaag ttttgaacgg
     3901 tttcgcatac tctgggccga acaatttatg ctcaacaaaa gaacgaacga ttggtgctgt
     3961 accttctggt cgaagcgtca catggcgttc ccctttgtca taaaaatcat acatctcttt
     4021 tgaaacgata tccgttgtat cccctacact tcgggatatc acttcatagt gttcaaaaat
     4081 cggtgtacgc atttcattat actgataatc tctgaataat aaacgtgctg tttcttcgac
     4141 aaactgccat ttttctgatt cgcctggcaa aatatcattt gttccttttg gtctttggaa
     4201 actcatagtt ttctctccct ttcttagcta tgtaaataaa aaaatcaccc ttgttcatca
     4261 tgaacaaggg tgaatgaaat tcatacacgg taccacctaa ttttgaaaga tttctctttc
     4321 ctcttacgaa taacgctcgt acgcgggacg gcttttcact cgtccatctc cagagtgtct
     4381 ttaggctaat gaaactttag atcattttca gcctcgatga tcctctcttg taaagtttca
     4441 aaaacttact cgctctttca ttgacattcg atattactta taacctacta aaaaccagtc
     4501 tcaaagtcaa gagatatctg actatttccc ctcacaaaaa cgttttagtt cagctttttg
     4561 gtattttgtt ttagactaga caaaaaggag gaatcagcta tggcagaaat aagaatcgga
     4621 cattcacaag tctacgcaga acagcttgga ttaggagcaa atgccgttgg aggacataat
     4681 ctttttgacg gtttggaaga tgagacagga aaacaagtag ttcgtacagc tttgaatagc
     4741 gggatcaatt tgatcgatac cgcttatgct tacggaaacg gccgttccga agaactgatt
     4801 ggcgaggtac tgaaggaaaa agaatatgat cgctcacgtg tcgtcatcgc tacaaaagct
     4861 gctcatgtac caaataaagg gcgaacattt gataattctc ctgagtttct caaacaatcg
     4921 gtggaagatg cactaaaacg tctgcaaact gattacatcg acatttttta tatccacttt
     4981 ccggatgaaa gcacaccaaa aaatgaatca gttgctacat tgcacgaatt aaaagaagca
     5041 ggaaaaatcc gagcagtcgg tgtgtcaaac ttcacgttag aacaattgaa agaagcgaat
     5101 gcagatggat atgtcgatgt agtagaagat aaatacagtt tgatccatcg gcaggctgaa
     5161 aaagaattat tcccttatct tgaaaaaaac aaaatcagtt ttgttcccta ttttcctctt
     5221 gcttccggtt tgctgacagg aaaatatgaa cttggtgagg aaaaacaatt tggtgaagga
     5281 gatcctcgaa aaagaaatcc agatttccaa ggagaacgtt tcagagaaat tttaaccgct
     5341 gtcgatgtac ttcgccccat tgctaaaaga taccaagcca ctcctgcgca acttgtttta
     5401 gcttggtaca tgaagaatcc acgagtgtct gtagttatac ctggagctaa gcgtccagag
     5461 caagtatctg acaatgtcca agctttagat ttgcatttat ccaatgaaga ttatcaaacc
     5521 attgatgaag catttcgtgg attttgatca attgacctta tcaatttata gtaatatgaa
     5581 aaaaggaaga tcagcactgt ttgatcttcc tttttttcga taagtttatg ctaatctaat
     5641 tcttgtaaag tagcgctcgg ctattcggat aagtcggaaa ctgattgaat ggaatcgtcc
     5701 aatcttgttc aggtacagag aactcaaact tatttactag ctgatcggaa aacacacgta
     5761 gactatgaag agtgatccat tctcctgcac agcgatgcat ttgtcgaaaa tttcccccgc
     5821 cttgtgcaat catctcatat tcttctttat aggaaatatc ttttgctttc cctacatacc
     5881 gtttgatcat aaaggaatct ggcgcttcaa ctgttcgttc atcatggtct gttccatata
     5941 aatctaaaat aacccaactg ccttctggta tacgataacc atctacttct acttctttca
     6001 gcgaaatcgc aggtagcatt gggaagaaag gataatagcg tctcatttct tgaataaacg
     6061 gatcttgtaa cgtcgaaaag tcatttttta attgctgata caaatctggt ctactgaaaa
     6121 gtgcatgtcc catcaatgcc gcccacacag ttagagcaac cgttggacgg atgatgttca
     6181 ataattcaac agctgccact tccagaggta aaagctgacc atctaaatct gtggcattgg
     6241 caaaagcata taatgccaca ttttcttttc cagccacagg attttgtcga gcttcttcaa
     6301 tcaagccttg cgcccatttt tctgattttt tgcgattttc gacacctttt atgtgatcaa
     6361 tcggagaagt aaacgttccg ctgatcatag agatttgata ttctgctaat ttttcgacct
     6421 catctttaga aatcgctgat aaattgatac ctgcccactc gcaaatagat gtaaagaaaa
     6481 ccatcttact taaatcaaat agttcaaatt gaccatgctg tgcttcaagt gcttgtgtca
     6541 gattcttatc taagatacga tgataatctt ccattctttc cggtgtcatt agatccataa
     6601 aaatattttt ccgatggtga tgagcagctc catccaaagt ctgtactcca ccttgaccaa
     6661 aaagtgtctt caatactaat tttggcatag caccttttcg tttgaaattc cttggatcat
     6721 aaaatacttt tgctgctgat gatccgtaga tcgtgatagc ttctttattg aaaatctttg
     6781 ctttgacgac gggagcgtcc gcttcatggc gtaactcctc caacatgttg tatccttttt
     6841 gatacaattt ttttaaatct gtgattttga tatctactac tggtacttct ttcatcttca
     6901 gcaacctcct ataaaaaata acttgtataa tttaagctta actttttttc cgactgctga
     6961 caaaagaagc gctttttgca gacaaaaaag ccacttaagt tagagtttat cagtctaact
     7021 taagcgacgt gtttcatagt ttttactagt gaaaaccaga ttactttatt tgtttttaga
     7081 atcaataatg atcgttacag gtccatcatt gacgagagaa accgacatat ctgctccgaa
     7141 ttgtccggta gcgactgtga tgccgcgact tctgattcct tcattgaatg attcgtataa
     7201 aggaatagct gtttctggtc gtgcggcttc cacaaagctt ggtcgatttc cttttttcgt
     7261 atccgcaaac agtgtgaatt gagagatact taaaatttgt cctcccacat cttctacact
     7321 aatattcatt tttccttgtt catcttcaaa aatacgcatt tgtgcaattt tttttaccag
     7381 atagtccaca tctttttgcg tatcggtttc atgaatgcct aataaaacaa gtaggccgta
     7441 gccaattttt ccaatttctt gctgatcgac tgacacagca gctttactta cccgttgaac
     7501 aactgctctc atatctgtcc tcctatccat tcgtccggcg tacactgtag acatcaggta
     7561 ctgctttgat tttatccacg atactcttta ggtgggataa attctggata ccaaccgtca
     7621 aatggatcgt cgccatttta tctttattcg tccgtgcttc aacgctgttg agatttttcg
     7681 ttagcgcatt gactgtttga agtacatcat tcaataagcc tgaacgatta tagccgtaaa
     7741 tttccaaatc tgcatcatac tcttttcgtg tattcgatgt atcttcccat tcgacttcaa
     7801 tcaaacgttc tgctacattt ggtttgtcag gctgaacatt cggacaatct cgacgatgaa
     7861 tggatatccc tctgccttta gtgatataac caacgatatc atcaccagga atcggattac
     7921 agcagcgact gatacgaatt agtaagtttt ctaccccttg aatgacaacg ccaccttcat
     7981 gacgtacttt cattttgtca ggttcttttt tcggctgatt catgatttct tctgcttctt
     8041 gcttttgctg ctcgattttt tgttctttac gttctttttc agtcagacga ttggccatcg
     8101 tcaaggggct aacttctcca taccctacag ctgcatagag atcatcttct gtttgataat
     8161 taaaacgatc gagtgcttct tgcagtttgt tcttcgtcaa aatatcttta ggcgtaaatc
     8221 ctagatctgt aatacatttg accacggatt cgtggccttt gatcacattt tcttctcgat
     8281 cttgggcttt gaagaaacgt ttgatcttat ttcttgcttt gctagtagca actaatttca
     8341 accagtcgcg acttgggcca aatgaatttg gagaagtcat gatctcaatg atatctccgt
     8401 ttttcaattt gtaatcaagc tgcaccattt tgccatttac ttttgcacca gtggttttgt
     8461 taccgatatc tgtatggata ctgtatgcaa agtccaatgg tccggatcct tttggtaact
     8521 cagtaacgtc tccttttggt gtgaagacgt agactttatc actaaagata tctcctttta
     8581 cgccttccat aaattcagaa gcatcatagc tttcgtcttg aagttcgagt atctcatgga
     8641 accagcttaa ttgtttcgtc ataccatctg gttctacttt ttcatttttt ccttctttat
     8701 aggcccagtg tgcagcaacc ccgaattcag cgatttcatg catttcctgt gttcggattt
     8761 gaatttctac cggatttcct gccggaccga tcacggtagt atgcaaagat tggtacatat
     8821 tagcttttgg catggcaata taatccttaa atctgccggg cattggtttc cattttgtgt
     8881 ggattgctcc taatacagca taacaatcct tgattgaatc tacgatcact cgaatagcta
     8941 gcaagtcata aatttcgttg aactgctttt tctgatcttt cattttacga tagattgaat
     9001 aaatgtgttt tggccgtcca tagatttctg caaaaatccc cagctcttct gtcgctattc
     9061 gaatatcttc aacagtacca cttacgtatt tttcacgttc ttctctcttc gtctgcatca
     9121 agtggacgat acggtaatac tgttttggat ttagataacg aagtgctgta tcttctaatt
     9181 cccatttgat ccggctgatc cctagacgat gtgcaagagg cgcatagatt tctaaagttt
     9241 cctgagcgat ccttctttgc ttatcttctc gcaggtgctt caacgtacgc atgttatgta
     9301 gtctatcagc taatttgacc atgataactc gtaaatcttg agccattgct agcagcatct
     9361 ttcggtggtt ttctgctagc tgctcttcgt gggatttata ttttattttc cctaatttgg
     9421 ttacgccgtc cactaacata gcaacgtcat cgccaaattc ttctctcaga tcttctaaag
     9481 tgatttctgt atcttcaaca acatcatgta agaagcctgt agccactgta tggggatcca
     9541 tttttaaatc tgctaatata cctgcgactt ggataggatg aataatgtag ggttcgcctg
     9601 attttctaaa ctgtccgtca tgtgcagcag tcgcgtattc gcatgctttc tcaacaaatg
     9661 ctacatgttc gggacccatg tattgactaa cgagctttat gacaccgggt cccgttaaaa
     9721 tttcttcttt tgtcacagga atcgatcccc ctttcttttt tcggtaaaac gggaatcggg
     9781 acaaatatct gtttgataat tgtcccaaaa tccgtgcagt tatacgtttt tttacggaaa
     9841 tgttcgcttt ttaaaagctt tttcaaacga tttttcgtat ccattgtata aataattgtt
     9901 tctattatac aaattgttga ctattttttc aattctttct gtcgatactc ttttattaat
     9961 gaagaacgtt agctgaaaga aggattcatt aattcccatt gatagctagc agcacttaat
    10021 aaataaagag gtgctgtttc tgtacgaagg attcttggtc ctaatccaca aagtatccct
    10081 ttgtgttcta caaaataatc gacttcatcg cgggataatc caccttctgg tccaaagaca
    10141 gctaagattc gactatctgg ttttgtttca ctaagaattt tggctgcatt tgatttttcg
    10201 ccttgctttg ctgattcctc ataagcaacc aagaccacgt cataatcatc tagccgctca
    10261 aataaatctt gtttcgtttc caataactct actgatggca cgatctgccg atgggattgt
    10321 tccgctgcct cttgagcaat cttttcaagc cgttgttgtc gtttcatacg tttttttgca
    10381 tcccatttaa cgacagaggc tgatgccggg aaaccaagaa aagcttgcgc tcctaattca
    10441 gtggatttct gaacgatcca atccagcttt tcccctttag gatagccact cgctatggta
    10501 atcgaacttg gcagttcctt ttgattttct tcttttttaa tttctttcag ataaacagcc
    10561 tcttcagtaa tctccgtgat ttctgcaaca atggctattt gatcattgaa tgccaaaaaa
    10621 acttcatcct cgggttccat cctcataacc cgtacaatgt ggtgatagtt ttccccttct
    10681 aaaacaaaat agtcttttga ttcatatgat tcttttaaaa aataccgctg catctattcc
    10741 acctctggtt ttttcaaaat aattgcatac cagtcttttt gctggaaaat ttgatccacg
    10801 ataaaccctt gttctaacat ggcatccaga accatttgct tcttctcttc aatgattcct
    10861 gaaacgatca gcgtgcctgt ttgcttcagt aaacgccaag catctgggat catcaataag
    10921 atgatatctg ctaaaatgtt cgcaacgatt acatcacttt caatctcgat tcctgtcaaa
    10981 agatcgttag ctgatacatg gacatctttg gctatttcat tcatatccat attttctttc
    11041 gccgaacgaa cggctacttc gtctaaatca aaggcataga catcttttgc accaagatat
    11101 tttgctgcaa tacttagcac gcccgaccca gttcccacat ctagcaatgt ttcaccacca
    11161 cgcaagactg tttctagtgc tgtcaatgtc aaggaagtcg tgggatgagt gcctgtgcca
    11221 aatgccatac caggatctag tgtgataatt ttttctagag gatcttctgt ctggtagttt
    11281 tcccagcttg gcgcaaccgt cagcattcgc gtaatacgca ccggatgata gtatttcttc
    11341 caggctgttg cccagttgct ttcctctact tcactaactg ttactttatt ttttccaata
    11401 gctagaccaa actcaggtaa ttgtgtgata cgatctttga taaaaggcaa gatttccggt
    11461 aagaaagtcg tttccggaaa ataagccatg actagtgcgc cttctttcag cgatgtaaaa
    11521 tcatttttat ctaaaatctc accatattga tccgactgaa aatgttcaac atctaaagaa
    11581 tcttcaatcg cgacaccact cgcccctgcc tccatcaaaa tattagcaac tgcttcgact
    11641 gcttcacttt cagtttctac ttttacttca ttccatttca tatctgtcac tccttaataa
    11701 cttgggtacg gtaaataagt atctgcttca ttttgctgtt ttacttctac taattctgct
    11761 agttgctgat tatcccagtg aagctcgaat acagcttcgt catcgtcttc atccaaaaaa
    11821 tcaagcaaat cgctttgccc ttgagctaat acttcattta gatattccac taaagaatca
    11881 gctactgatt ttgccaaccc tttttttcct tcataaggga ttactgctag ataattgcgt
    11941 tcatcaaaaa cagatttctg aggattaaaa agaagtacgc cgtcttcaaa ttcgataatt
    12001 ggttcttgcg aaacgatccc ttctgcatca tcaatctcaa attgtgcttt attttctgca
    12061 aacaagcgaa caacgatctc gatcgtatga ttttttgtat cccaatctaa agctacatca
    12121 tagtctgtca cttgtttttc aattatttca tctaaatatg ttaatagatc cttctttttc
    12181 attttctttc ctccaacccc aatctttctc gatttggttt tttctttgta aagagatata
    12241 aggatttccc gacacaacaa aacgcaacgg cttttctgtc catacacctt tgtttgggat
    12301 accgattcta ggcaaagcat tgatcttttt tggtgtcttt tttttctcaa aaactaagcg
    12361 taacgaacta tctgctatag actgaccata aagttcttga ggaattgcta atgcttccac
    12421 taattttccc ggaccgttgc tgatatctgg accagttttt ccgccacgat ttttactcat
    12481 ttgatcaatc attgcggcgg gttcgattgc acgtatcatt actccttgag gaatcccttc
    12541 tggctgagta atgatgttca aaatccgatg agtatgcatc gtgtaaaggt agatcgtacc
    12601 tggtttttca tacattgccc gcacacgcgg tgttcttctt aaaccatagc tatgggcggc
    12661 ctcgtcttct ggacctaggt atgcttccgc gtctacaata tatccagcta atcttccttc
    12721 tggtgtgtca tgctccaaat acatccccaa taaatcccgt gccacttctg gcgtcgtttt
    12781 attccaaaaa aattcatcta tcttttgttt atccgtcatt tttctcacct actttatcat
    12841 acacaatatt agttggataa gctatgaaaa caaaacaaca gaaggctgtt ctttctaggc
    12901 atcttgctaa atatgatata attaagggac gtaaaaacaa gaaggaggaa aatcctatgc
    12961 aacagccatt ggcttatcgc atgagacctc gaacgatcga tgaagtcgtt ggtcagcagc
    13021 atctagtcgg ggaaggaaaa atcatacgcc gcatggtcga tgctcgcatg ctctcttcca
    13081 tgattctata tggacctcct ggtactggaa aaacgagtat cgcaagtgcg atcgccggct
    13141 ctactaatta tgcatttcgc atgctaaacg ccgctacaga ttcaaaaaag gatctacaag
    13201 ttgtagcgga agaagcaaaa atgagtggta cagtgatcct cttattggat gaagtacacc
    13261 gattagataa aacaaaacag gattttctgc ttcctcattt agaaagtggc cgaatcatct
    13321 tgatcggagc gactactgag aatccttata ttaccatcaa tccagcgatc cgcagccgta
    13381 cacagatctt tgaagtcaaa ccattaacag aacaagacat ccagttggca gtcgaacatg
    13441 ctttaaggga taaagaacgc ggccttggac aacaagcaat ccagttagac gaagatgctc
    13501 ttcttcacct ttcgcgtgca acgaacggcg atttgcggag tgcattgaat gggctggaac
    13561 tggctacact atcgactcct tcagataaag aaggaaggat tcatcttaca ctgtcgatca
    13621 ttgaagaatg tgtccaaaga aaagctctta cccatgacaa aaatggagat gctcattatg
    13681 acgttatttc ggcttttcaa aaatcgattc gtggaagtga tgtggatgct gcacttcatt
    13741 atctcgcccg tttagttgag gcaggtgatc tggcaagtat ttgccggcgt ctgatggtta
    13801 tcggttatga agatataggt ttaggtaatc ctgctgctgc ggctcgtaca gtgaacgctg
    13861 tattagctgc tgaaaaattg ggattgcctg aggcacgtat cccactagca gacgtcgtcg
    13921 tcgatctttg cttgtcgcca aaatccaatt ctgcctacat ggcattagat gcagcacttg
    13981 ctgatattcg agagggcaaa gctggagatg ttcctgatca tttgagagat agccattaca
    14041 aaggtgcgaa atcactcaac cgtggcgtgg gttatcagta tcctcatcat tttgatcagg
    14101 cttgggtcaa tcagcaatat ttaccagata aactgaagaa tgctcagtat tatcagccaa
    14161 aagatacagg gaaatatgaa caggctctgg gtcaacagta ttaccgtata aaagagtgga
    14221 aaaagcatcc tccaaaaaac tagacaagaa aacggttctg tttctgcgag ctctctcatt
    14281 gctcttttta aattcatatg ctactataga ggtgggaaat ctgtgatgtt cgaattgtcc
    14341 tctttgtgtg ttgaccgaac atttttattg atatcttggg acccgtcttg tgtcactgct
    14401 ggtaacatgc cttatcattt ggtagttcga agctacgacc atgggaccca cctgcttgaa
    14461 ttcgcgggat caatactaca ggacaaacaa cggcattaac ggagattacc atcttttaaa
    14521 gaataaagca atttgctctt ggcaaattgc tttttctatt tgttgcttat tttggaggat
    14581 ttgttcatga ttaggatttt aatgattatc gccactttag tattattatt cgttagctat
    14641 tatttattta ataaacagga tatctttttt gtattgatca acaaaaatga taaaaatcaa
    14701 ggattcctac aattttacgg agctgcatat gctgttttag gcgtaatggg tattttaaca
    14761 gcttttttca atcaacgctt tattgccttg gtctttttac tgatcgtaat tattgtttct
    14821 gccaccttca gtatccgatt tgcaaaaaaa attgctgaac caaagcagta aataattctc
    14881 tttttcccta aagtgttcta caataaaaga gtacaatacg attgacttga ggagtgaatg
    14941 cttatgttac aacaatacaa aaaaatcatg gttgccgtag atggttctga tgaagctgaa
    15001 ttagctttta aaaaggcggt caatgtagct atccgaaata acggagaact tctattagca
    15061 catgtcattg acacacgttc tttccaaacc gtttcatcct ttgatggaat gcttgcagaa
    15121 caagctactg aaatggcaaa gcaaacattg gcagattatg aaagcaatgc gaagaaagct
    15181 ggcttgaata atgtaacctc tgtcgtagaa tatggctcac caaaacaaat tattgccaga
    15241 gaaatcccag aagataatca agtagatttg attatgttag gagccacagg attgaatgct
    15301 gtagaacgtt tatttattgg ttctgtttct gaatatgtta ttagaaacgc agcttgcgat
    15361 gtgcttgttg tccgaactga tttagaaaac caattgccag ctaaagacca caccaaagct
    15421 taacacgtag acgacctgcc aactatcggc aggttttttt gcacaaaaaa ccgtgataaa
    15481 aacaaaagac gcggcaagca ctcatcggtc tttataatgt ttaaaataaa gggaaaacag
    15541 tcaatgagtt caagtaaaat aaaaaaaagt gaacaccagg gcgattagtc gccctggtgt
    15601 ggtttaaaac gacctaagca tgccaaagct tattggttcg tcaatgtgga agtttccgat
    15661 gaatctgata cgaaaaaata tgaagtatcg atggcggata ccgattttat cgccaaacta
    15721 aaattttatt tcgggttcgg gtaccgcgag aaggcaagtg ctacattcgg taaatcattc
    15781 aagaattgat gaaaggctac cgcttgccat tgcaaccaca acgatactcc tgaacgcgat
    15841 gttggtgaga tttccaaaga gaagagctgt ttaatgcaac ggtattaagc aaatggcaaa
    15901 aggaagtcat gtagacaaag ttttttatca aacaccatat gatttcgcct gaacgctggt
    15961 ttagctcaga atacaatgat gtggtccacg agacattacc ctcagtcaga taaaaaaagc
    16021 tctctgacag aattacacat cgataaatat taagtaaatg tcagcgctag ttgaactttc
    16081 tgacactttc aggtacactg gaaaagacaa ctacacgaag gagggaaaat actatgatgc
    16141 gcatgactaa aataacaagt aatgaaaatt accaacgata ccatatcgca tttagccatg
    16201 caattggttt tacagttgct gttgacggga caagtttgtc caaaacagtt gaattcacgt
    16261 aaaatcaatg gccatctcaa ggtatttttc tcatgataat ctgagtttaa ataacacagg
    16321 ccatggataa ccatggcctt tttgagtggt ctcaaggaga gagatcatga tcatacaagc
    16381 aatttctatt aaaaaaacaa tcggcgggaa tccattattt gaggatttat cctgtcaaat
    16441 tgaagctggt gaaaaaattg tcttagtcgg tatcaacgga actggcaaat caaccttttt
    16501 acaaatcttg accggcagag aaggtgtcga cagcggtgtt atcagtcgca aaaaaggctt
    16561 aaagatcggg acggtcgaac aagaacttac agtttccgaa gcgaccgtca accactactt
    16621 gctccattct gccgctgaga tacaggacct caaacagcaa atgagtcgct acgaagcatt
    16681 gatgacagaa ccagatacaa acttagagaa gtgtctggcc cgttacggtg agctgcagca
    16741 tcgctttgaa gaactaggcg gttacgtatt agaagaccgg attgcagcga tcttgcaagg
    16801 cttagggatt ccacaccgtc aggaagcacg gttaacagaa cttagcggag gcgaacgagt
    16861 taaagtcgct ttagccaaaa ttttagcagc cgatgccgat ttacttttac tcgatgaacc
    16921 gaccaatcat ttagatctta acagtattcg ctggttggaa aactatttac agaatacaaa
    16981 aaaatcggtt ctggtcgttt ctcatgatcg gcaatttctc gatcaagtca ccacaaaaac
    17041 atgggaactg gaagaaggtg cattgattga gtatcccgga aactacagcc gttttcgagt
    17101 cttgaaagaa gctcgcttgg ctgagctgac aaaaaattac gaactgcagc aaaaagaagt
    17161 ccagcggttg aaagtcatga ttcgtcgttt tcgtcagtgg gcacatgaag gagacaatga
    17221 gtccttcttc aaaaaagcca aagaactgga acgccgcctg gcgaaactga cactcgtcaa
    17281 accgccacct cccccgaaaa atcggctgca aagcttgtct aatggtggaa aatctggcaa
    17341 agaagttttc atcattcaaa acttgcacca gcagtacgct gatcaagtac tattcaaaga
    17401 cagcagtttt gcagtctatc gtggagatca tttagccatc atcggcgaca atggtgcagg
    17461 aaaatcaacc ttactcaaat tattactcgg tgaggaaaag ccttacggtg gcaccatcaa
    17521 attaggtagt agcttgcaaa tcggttattt gccgcagcag ctgcagtttg ccgatcccga
    17581 tagccgtttg ctggcttacg ccatcacatt gactggaaac gaagaagtcg ctcgacggca
    17641 attagccaaa agtggctttt atcaaagcga tgtgggtaaa cggatcaaag atttatccgg
    17701 tggtgaaaaa atccgtttgg cgttgctgaa actcttttta gaaaaaatca acgtcctgat
    17761 tttggatgaa ccaaccaatc acctcgacag ctatgctcga gaagaaattg aagaaatgct
    17821 gcaagactat caaggaacgc tcctagcagt cagccatgac cgctactttt tgcagcaaca
    17881 cttccaagaa gcactcgtta ttgatcagga gcagatcagc cgccagccgc tcggccaact
    17941 gattgactag gacgtttttt cgcaacaaaa aacttgattt tcatacctgt gctttcacga
    18001 ttcaggcaaa gtacaggtgg aaaatcaagt ttttattatt cgatcaatga atgtttcgga
    18061 tcggttttct agctaaacat aaagcgaaga ataaagtcga cagcccacga ggctgatcta
    18121 aaaatgtttt ttctttttac atatgtaatg tcccttataa agatgaatga aaataaaata
    18181 ttgggatttt cctgaaaatc aataaataaa atgccagtct tttccctttt gaccactttt
    18241 ccaagcaaaa ttccctaaga agatcctgta aaaaagaagg gaaattgaag ttaaattacc
    18301 attttgcggt cttctccgca aaaaaaacac atgcaagaat tccctcttgc atgcgtttca
    18361 tcttttatcc taacgaatgc tttggcttag ccgcgcagac gctccacatc acgggcaatc
    18421 attagttcct catctgttgg gatcactaat actcttactt ttgattcttt tgtagagata
    18481 tctacttctt caccacgcaa ttcattcaac gctggatcga tttcacaacc aaaccaagac
    18541 atacctttga tgatttcact acgtacatgt ccgtcattct cgccgatacc agcagtaaag
    18601 acaattgcat caacaccatt catcgttgtc acataaccgc cgatgtattt tcggatgcgg
    18661 tccacgaaaa tgtcataagc taaacgaata tgttcttttt catagttctt ttcaagatca
    18721 cgcatatcac tagacaatcc tgttaggcct agcagaccag atttcttgtt caaaacattg
    18781 atcatttcat caatatcaat ctctaatttt tccattaaat atgggagaac agctggatcg
    18841 atatcacctg aacgtgtacc cattgtcacc ccagctaatg gtgtgaatcc catagaagta
    18901 tccatggatt tacctccatc gactgctgtg atcgatacac catttcctaa gtggcaagtg
    18961 atgattttta gttcttcgat cggtttgttc aataattttg ccgcacgttc cgacacataa
    19021 cgatgacttg tgccatggaa gccatatttt cttactttga aatcttcata ataagacacc
    19081 ggtaagctat ataaataatt ttgctttggc attgttgagt ggaaagatgt atcgaataca
    19141 gccacactca aaatgtcagg taaaatcttt ttaaacgctt tgatccccat taagtttgca
    19201 ggattatgca aaggagcaaa ttctgctaat tcagcaatgc ggtccattac tttctcatcg
    19261 atcactactg aatccccata agtttctcct ccgtgaacaa cacggtgccc tactccggtg
    19321 atttcatcat aagagcttaa aatttgcagt tcaatcaact gatctaacag catttttact
    19381 gctacttcgt ggttttcgat atccatgatt tgttcgaatt tttgattatc gccgtacttg
    19441 atggtaaaaa tagaatcttg taaaccgata cgttcgacga tccctttcgc aactacggtt
    19501 tcttctggca tttgatataa ttgccatttc aaacttgaac tcccagcatt gattgcaatt
    19561 gttttagaca tatgactctc cttctcgttg aatgattaca gagcagatga tttccagttt
    19621 ttaaactcgt tgaaaaattc cgtcaccttc tctggttctt ttaatgaagc aagtttcaca
    19681 agcaagactt cttttacttg ttgtgcttgt ggtccttttt tctgtaagat caaaatactt
    19741 ttttgcgatt gtttattacg gaacaattca tctggcaatt gaatcattcc ttgaagataa
    19801 ccttcttctt taaaccattt tttgatttct tcactttgat ccgtttctaa aaatccactc
    19861 ggcatcaaaa acaaaccaaa accatccggc ttcacatatt tcattgattg ctctagcaat
    19921 aaatgatgtg cataactatg gccttcttcg gtacttgtta aaaactcttt tgctttttcg
    19981 tcattcggat aataaccgat tggcaaatcg ctgattgcaa aatcgacagg atctattaat
    20041 aattcctgca acccgtcttg atgaaagtat tgaacatttg cttgtgtcaa atcacttgta
    20101 gaagctgcga cagccaacaa tgtatcatca atatccacac caattccttc tgtttggtac
    20161 cccgcattcg ataggtttaa aagaactgtt aataacaagt tgcccattcc tgccgcaata
    20221 tccagaatct taaccggtgc ttttttattt gtgtatagtt gttcaataag aaaaacaaat
    20281 aaaaaaccga tactatcagg agtgagttga tggttcgcct gcaaatgttc tgtctggctg
    20341 cccttcaata aaagtaattg agacagtctg cgccattctt ctggctcgaa agaaagtttc
    20401 ttcaactctt cataaagagc tgtgatccgt tgcgttgtct cttttgtcgg cactccatcc
    20461 accacacgta cttgataatc atctattaaa ttttctgcat tttcgacata ggcttctaaa
    20521 aaggaagttc ccaatgcatt ctgtaaaagc tgaatagctt ccaggttttg attaaaccct
    20581 tgttccattt tttctggtaa catggtgcac ctcatttcta gatatattat aacaattttt
    20641 tcacctgttt aattctatcg aacaattgac aaaatttcaa tcgttttcac tgttttttat
    20701 ttgtgaaacg gcaaacattt ttttattaaa aacagtgtta taacagaaaa gagggcgttt
    20761 tcacgaacat tccatcattc tgtactagta tctgtatcat tttttacttc ttctgtgaac
    20821 cgaaagataa acggtgagac ttccacctct aagaataggg actgttgtac ccactgatat
    20881 gttacagatc cagttgtgta gtaaaaaact ccttccgaac tttcggcaag cgtagactgt
    20941 tccgataaag ccatctgctt cattattttt gctatataat aatttttgat tcgtatatta
    21001 agatccattg acaaacggta gctttctaat gctgccatca ataaaaacgt acataaaaga
    21061 aataaaagca agacagaaaa taaaatagag cccttataac attttctact gatcgttttt
    21121 tgtccatatg gcatatccat cttctccatt ttcaaagtgt acataaaaag aaattgtgtg
    21181 ttctttttcc aataattgaa gctcagtgat acttgttaac ataggttgat gtccgccgga
    21241 agttcttctt ttacgtatca ttgcctgata tctttcgatg atatactgtt tatcctcgat
    21301 cgtgtagtgc agttgctgat ttttcacaga gacattgtga ctgctcttga gttcttcttc
    21361 taattgtgct aaaaatatta gccactcttt ttgatcttta cgctgcaaaa attcacttac
    21421 ttggatggtt tgctgaatca aaagattgaa tgtgagcaat agactgctca atatcatcag
    21481 tccaattaga cattcaatca gcgtaaatgc tggaattact tgttttttca atactgatcg
    21541 cttctttctc catattgaca aacacttttt cgattactcc attattatcg acaatttgga
    21601 tatcgtatgg gatcatttct tcccttcgct ctggtctttt gtgatacatc cgctgcgtct
    21661 tgatatcttc ataaatcatt cgaataaata gcagttcctc cccacttttc ttttctgcct
    21721 gaagcaaaaa cagctggcca aagctcacta agtagacaca gacagaagcg atcataagcg
    21781 ctgtaaggga ctccaacaaa acaaatcctg ttttattcaa ttctttttgt atagcgacca
    21841 ctccctatct ggaattgata cgtaatcgtc cgctcttttt cttcccaata aaattgataa
    21901 gcttttagtg aactttcatt cccggttcta gcagcaaaag taatagaagc atggcgtttc
    21961 aaggtaatct catcaggaat atctaatact tcccaattta ttttatctgg attcggaaca
    22021 tgaaaaatga tttgattctc ttcactattc caataaaagc ctgtttggat ctgattcaca
    22081 atagcgattt tttgcgtaga atagattcgt ttttcaaatt ggccgaaaaa ttgatagact
    22141 gccaattcat tcttccatgc agaaaatgcg agaactggaa aagaaaggat cgttgtaagt
    22201 aataaaatca gtaagagtga ttcaaacaac gtatatcctg atgctaaaac cttaccttca
    22261 aggccgctga tagatttctg catgttctcg tttgatatag ccttcattca acaattcctc
    22321 cacagtagga actcttccat tattttgaag cgcatagagc tctttttgtg tgtctaccaa
    22381 ctggatgatc gcttcacgac tttcttggtc tacatgattc ctataacgcg acaaattagg
    22441 gacgaacaac aacactaata cactgatgat cagtaagacg attagcattt cgatcaatgt
    22501 aaaaccgcta taataagata tttttctctt tttcatggca aaacctcctc tatgcttccg
    22561 taaacaggta ataaaacagc ggcataaact aataaaatca aagccgctac gcccaaaaaa
    22621 atgattggct gaataaaatg catccagtga tgaagacgtt tgaaaagctg ttttcttgtc
    22681 cattcgctgt aaaaaatcag ttctttacct aaacttccct ttgcctctcc ctgtaaaaca
    22741 attttactga attcttctga tagaaaacga taattctgga actgctcagc caatgacata
    22801 cctgcctcca atccttgaat caacttagag gcaagatgct gaatcagact ttcttgtttc
    22861 gtttccttta agcagactat gatttgcctt aattcgaaac cttcatgaaa aagctttcca
    22921 atctctagtg acaaataagc cgattgatac aaggaatata tcggtccaat caataattgt
    22981 ctgcttatcc attcactctg atcaacagga gacaacttca tcagacgata ccttactagt
    23041 aaagccaata gagtaaccgc taggatgaat agtagaacat accaatgaaa gacttgtatc
    23101 agctgaatac cccaatgctc ttgcatgacc attcctgaac caataagctg aggcaaaacg
    23161 aacaaccgca tactaaacaa gatacctagc aaaaaggtga gcaatagcat cggatatgca
    23221 agcagctttt tcaaatcatt gcgaaatcct tcaacaagcc gaaactgttt ggcaatttcc
    23281 aacaaagttg tcggcagatt cccatgacgt tcagctaact cgatctgtac cagttgctct
    23341 gctccatatc ctatttgcga aagtacctca ctaaacgctt gcccttcctt tagctgatgt
    23401 tggatatttg ccaaaataac aggtggaaac gaacggatcg tgagtaataa ataaagggat
    23461 tcttgcagac taaaaccaga taaaagcagt tctcccatgg tttgtgcaaa ctggttttgc
    23521 tggcttcttg ttaatccttt agaatagttc tgactgccaa atattttcct gagcttcttc
    23581 ataactttcc tcccatgatt tttttactga ttcttcctcc ataaattgat aactccataa
    23641 aacagaagga tggtgattgg catcttccaa aagttcttga taaatgacac cagctaaaca
    23701 ttcagatagc tcttcttttt tctttgtcag ttcatggatt ctgaccaatg taccttcaag
    23761 attgcgtgca tgtacggttg caaaaacccg gtgccctgtt agtgctgctc ttaccgcagc
    23821 atgtgcagtt gattcatctc ttatctcgcc aatgattagc aagtctggac gatgccgtaa
    23881 tgcgagtctc aaaagtgtat cataagtctg tgctattttc ggattgatct gtagctgcag
    23941 aaaaaacggt tcttctatct ctacaggatc ttcgatagta atcacttgca atttttcttc
    24001 caaagccaac cgatacatta gagaagtctt gcctgatccg actggtccgc tgaaaaggta
    24061 taatccacgt ctgacggttt tctgtaaaat agttgcaaaa tctcgagaac taaagcaacg
    24121 atggggtgtt tcaaaagtac cgtataatag ccgaatcact aaactctctc gttgaaggta
    24181 atctcctaca gaagacaacc gcagacgaac ttcctgtttt tctaaagaat aagtaaatgc
    24241 acctaactga gcttttcttt tttctccgat atccatctgt cccaaatact taaatcttga
    24301 gatcagttgc tgtccatctg ccgaagagac tttcttccag aatgttcgtt tttttccata
    24361 gcgaaaatag atgtaaaata cttctgaagc tggcaatata tatacgtctt gggcatgttt
    24421 ggcaaaactt tcacttatca tttgatccga taatcgtttt atttccatta ttctcccctc
    24481 cacaaaaaac atacgtcttt ttgatctttt tgtttcaaat aaaatgaatt tacaaaaaaa
    24541 tattggcaaa aaccgaattt atgttatact tataaaaatt tatcttggga ggcactccat
    24601 tggaaacaac attaaggaaa tatatcgaag tagcaagtaa aaaaaagcct gctgacctag
    24661 tgatcaagca tgctaagatc gtcaacgtct ttacgaaaga aatcatggaa aaagatgtag
    24721 caatctgcga aggaatgatc gtaggaatcg gtgaatacga aggaaaaagc gtacataatg
    24781 ctaatgggca atatctagtg ccaggattca tcgatggcca tgtgcatatc gaaagctcac
    24841 ttttatcacc aagcgaattt tcaaaagtat ccttgcttca tggagttact actgtagtga
    24901 cagatcccca tgaaatagga aacattgctg ggagtacagg tcttgagttt atgatagaag
    24961 atgcaagaca aacactcatg aatatctttt ttatgctacc ttcttgtgta cctgtaaccc
    25021 cttttgagac caatggtgca aatttaaatg caaaaaaatt agcccctttt ttacaaaaac
    25081 ccgaagtttt aggcttagct gaagtcatga actatcaagc agtagcaaat aatgaaacag
    25141 acatcctaga aaagatccaa ttgatgcacc agcataagaa aaaaattgac gggcatgcag
    25201 ctggtatcgg catggaagag ttaaatgttt atcctgcagc aggtattcgg acggatcatg
    25261 aagctacaac tgcaaaagaa gctaaagaac gtttggatct tggtatgtat ttaatggttc
    25321 gagaagggac cgttgcaaaa gatctggcat cgctactacc ggcgatcaca cctgaaaatt
    25381 caagaagatg cttctttgtc accgatgata aactgatcaa tgatttagta aaagaaggat
    25441 caatcgatca tatcgtccgt caagctattg cacttggaat cgatccatta caagccattc
    25501 agatggcaac attgaatgca gcagaatgtt ttgatttgaa aacactaggt gcgattgccc
    25561 ctgggtatca agctgatttc ttcttaacag acgatcttac caagctgcca attcatgaag
    25621 ttttctataa gggaacatta gttgttcaat ctgggcaact ccaagaccat ttatttgctg
    25681 atcattcaaa ttcatatacg tgttgtctgc caaaactaaa cgctcaacca ttaaatccac
    25741 attcgttaga gctgcttctt tcttcttcac aagcccatgt gatcgaaatt atcccgaata
    25801 gtctagtgac taaagatttg gtagaaaaag tggaccgaca aggcgaacga ttcattcctt
    25861 caatcgaaaa agaccaatta aagattgcgg tgatcgagcg tcaccatcaa acgggaaaca
    25921 ttggattagg catagtgaaa ggatttcagc tgaaacaagg agcgatagcg acaacgattg
    25981 cccatgactc gcataatcta gttgtcgttg ggacaaacga cgaggatatg ctgtatgctg
    26041 ctaatctttt gattcaaaaa ggtggcggga tgaccgttgt caacaatcag caggaaatag
    26101 cttgtgtttc tcttcctata ggcggaatca tgacacagga acctttttcg atcgtctatg
    26161 aacagttgaa tgaattaata actaaagcac atcaactagg agcagctaaa acctttgatc
    26221 cttttttgac actttctttc ttaacattat ctgtcatacc cgaattgaag atcaccgaca
    26281 aaggattatt ttcattttct cgcttccaat tgattccacc ttgtggaaaa accgatgaaa
    26341 taaatacatt ttaaatcaaa taaatcccag aaaatacatt acatctgtaa tgttcatttt
    26401 ctgggattta tttatggata ttaatgtttt tatgaaagca gaaagttagt ttcacagact
    26461 tttctttttg gtcttatctt gaaattgagc ttctactcga taaatacgtt gaccttttga
    26521 agaaaatttt tcttcatact ccgtcataat attgccttca aattgacttt gatgtaaatc
    26581 taaccatacc tgtttgagga tcatgccata ttgagaaaaa cttgccagag aatactcaaa
    26641 caatccttga ttatctgttt tgaaatggat ttctccattt ggacgaagaa tctgttcatc
    26701 tacagcaaga aaagtcttat atgtcaatcg ccgtttttca tgacgttttt ttggccatgg
    26761 atcggaaaaa ttcagataga tttgatctac ttcgctgtca gcgaaatact ctgtcaacgc
    26821 tgatccatcc acatgtagaa gtttcaggtt aggaagcggt tgttcgatca acttgtctag
    26881 tgcaatcgaa acaacactga cctgcatttc tactccaata taattgattt caggatgggc
    26941 ttttgccatt ccagtgatga actggccttt ccccatacca atctcaatgt ggattggatg
    27001 atcgttttca aacaattcat tccattttcc cttccacaat gttggatcac taattacaaa
    27061 attcgggtga gccgctaaca tttccgctgc tcccggacga tttcggacac gcattattta
    27121 tctccattcc tttcctctaa ttaaaaaggt ctggccaaat ctccgcagag tttgggccaa
    27181 acctcagcca ctgaaaattc gttttaattg taaaatttct tcgttcatcc gtcgatgatc
    27241 ttcacgtttg caatatctgg tgatttcttg gagcaaattc atggccgcat accaatgaaa
    27301 tttttcaagc atttcgtcag tcgggcgtat gccataactg atcagccact gattccagct
    27361 agacagtggc acataatttc ctaaaatcgt tgcaatatcc accgcagggt ctgcaaacat
    27421 gattgaatcc caatcgacta gatataaata atttttacat actaaccaat tacgatggtt
    27481 tacatctccg tgtactactg ataagttttc cttatctatt gacgggatct gctcacgaag
    27541 aaaacgatgg acaatttgca gaaattgatt tttctggagt tcttccggta attctttttc
    27601 atatttttgc agcatcttct ccggcgtcat gacacgacca ccgatacggg tcagcatggt
    27661 tttaagagaa tttgaatgat gtaaatggta caaaacatca atcacgtcgt ttctttgacc
    27721 aatctcatta ggagtcagca agcgaccttc cagccactct tgtgctgtca aagtatcgcc
    27781 attccccgtt cgttttgtcc aaacaagttt tggtgtaatt ccttcccttg aaagagcagc
    27841 caacatcgga gtcgtgtttc gtttaataaa gactttgtcc tcatcacgca tgcctatgta
    27901 tgtttttcct gtgtcgcctt tgatgggacg taaccgccac tcttggtcta actgaaactc
    27961 catcatgcat tctcccgtcc tcttactctt tgttttgcag tacttattat tctagtcgcc
    28021 tgaacactac acgtcaagca catatacgtt ctttttcatt taattgccat tcaaaaaacc
    28081 agcttttctc aaaaaaaata gaacgaacag cattcttact gtcaacgctc tatttttcat
    28141 tctcattttt accctacaca gctttgtttt cagtctgaca tcttcttcaa acgagttggt
    28201 acatatagtt taacaaagag catagtaaca atcaaataag ccgctagcgg aacccatcgc
    28261 tgctgcccat ctaatacgac tgcagcaatc aaaccaaaaa taaccgacgc gataattaat
    28321 acccatgtga gtagatactg aatcgcctgc ttcttctgat tcttatggat tggataaagc
    28381 tgaacgagaa ccatatagcg gaactggttc gctaaaggta acagttggaa accgatcaaa
    28441 tataaaaata agaaaccgat aatcagacta aaataaaagt cgactactcc ggcaatcaat
    28501 aacccaccta taattactaa tcggaaataa agaccactaa aatcagaacc tcttgccaat
    28561 cttcttgcaa acaagtaaag ataagtgttt tttgattctc ttttaatatg agtcaatata
    28621 ggatccaaat agcgtcttct tttgatcttt gccgtaattt caggaacatc tgtgaataaa
    28681 ttgataaatt gatagatctg atgcaaccta tgttcttcta catgcaccat tttctcccaa
    28741 tccaatcctt tttcttgttg cttccacaaa aaccagtaat atagacttgc ctgtactaat
    28801 gcacctgcta ttcctgccca tattagccaa taaaatgata gaaacaaaaa gatcagagaa
    28861 gaaaaaaacc agcctaattt ccaataacga ataacttgtt gatctagtcg aaacaattct
    28921 gctctcttga tttgcagatg actgaatttc aaactccata atatgatcag atagacgaaa
    28981 aacatcgaaa aggattgctc cgtactaacc accactaatg gcatggccaa tccgcatgcc
    29041 aatatcaaaa agatcaacgg aaaaatgcaa gaatagcgaa aagctcgttc caaataggtt
    29101 tgcatttctt tttctttagg aagtaaaaaa acaatatctg ctgcctctgc aaaagtagcc
    29161 atcttaccca cgaataaaca gagcatccaa aatacagaga ttatgagccc tccaattgca
    29221 aacggtggct gcagggtttt caaccaatta gaataatata atcccacacc gccaatcaag
    29281 aaaagacaaa ctaaaacaaa atgatcgttc aatacataac gcatatattt catcattttc
    29341 ttttgatgtc ttgctaaacg tttttgaaaa aaactactca tgtgcctgct cctttgttaa
    29401 agcaatatag atttcgtcca aagaagaatc cggcagctga aattcttttc tcaacatctc
    29461 aagagaaccg tctgctcgta tctctccgtt atgtaagacc acaaaacgat cacaatattt
    29521 ttctgcagta gctaaaatat gcgtagacat caaaataccg gctccttctt ttttcatttc
    29581 taccatcaat tcaagcaatg cattgatagc tagtggatcc aagcctaaaa atggttcgtc
    29641 gatgatatat aaagaaggct cgatcaaaaa ggcgcataag atcatcactt tctgtttcat
    29701 tccctttgaa aaattagctg ggaaccagtc aagacgatta gataaccgaa aagtctccaa
    29761 caacttttct gctcttttca ttgctatatc cataggtata tcgtaggcca tcgcagtgac
    29821 ttcgatatgt tctctcaatg tcaattcttc atataaagaa ggagtctctg gaatataccc
    29881 aatttgtttg cgatattctt ccgtattttt ctttaaggat agatgattga tttctatcgt
    29941 tccttttgaa gcttccagca gaccgataat gtgcttgatg gtcgtacttt tccctgctcc
    30001 gttcagtcca atcaacccta ctatctctcc aggctgtaca tcgaaactga tatcttttaa
    30061 cacagggaga tgtccgtacc cccctgtgac atgatcgata attaaactca ttctttttcc
    30121 tccgccattt atttttattt attataccac gaagaaaatg acggtatttt gtttttttcc
    30181 ttacaatagc tttaacttat ggtaaagtta agctgaagta tttattcttt atagaaagga
    30241 tgttttatat ggaaaactgt attttttgca aaatcatcaa tcaagaaatc ccaagctata
    30301 aaatatatga agacgacaag gtctatgctt ttttagacat ctctcaagca acgaaaggac
    30361 acacattagt tgtgcctaaa cgtcatgtag cagatatttt tgaatatgat cccgaacttg
    30421 caggagaggt tttttcgcgt gtgcctaaga ttgctcaagc attggaaaaa gcttttccag
    30481 aaatgaaagg tcttaatatc ttgaataaca accgtgaact agcctatcaa tctgtttttc
    30541 attcacatat ccatttagtc cctcgctaca gcaaagagga tgatttttct attcactttg
    30601 tcaatcatca agatagttat ggatcggaag aactaaaagc tattcaagaa acaatcgtaa
    30661 agcaggtgtc ctgcgatgat taaaaacttt ttcaaaggac tgttatttgg agctgctgct
    30721 ggcactgtcg gaggtctgtt attggcgccc aggagcggga atgaaacaag aaagaaactg
    30781 atcgaagaat tggaagaagc aacaactctg acaaatgatt taaataacag cctgaatcat
    30841 tttaaaagcg cattgatcga aacaaaagaa acagctgaat ctgtgattcc tgctttccaa
    30901 gaagcaatca aaaaagatat cgaagatttt aaattccaag ccgaaccacg tatcacacaa
    30961 atcaatgaac aagtagaaaa actaaaagct gatctgccag agtatcctga acaaatataa
    31021 taatgattat tttctcaaca gatttaacta tataagtgag aaaagtttgc gtatttccta
    31081 ggaatgtgat gaaattttga attttgtttg aaactaccac gacaacgttt ttaaaggaaa
    31141 tgggatatgg tatagttaat atataaaaat aataactaat tacaggagtg caaattttaa
    31201 tgaaaaaaaa atcaattatt ttggcagcta ctagtgcgtt ggctgtttta actctagcag
    31261 cttgttcagg cgatactaat aaagatatcg caacgatgaa aggcggaacg atcactgtat
    31321 ctgatttcta tgatgaagca aaacttgaat catcgaacca atccttagta caacggatga
    31381 tcatttataa agtattcaat aacaaatatg gcgataaagt aaccgataag caagtggatg
    31441 cagaatacga caaacaagcg aaatcattag gtgatacttt tgaatcacaa ttagaagctg
    31501 ctggatatac gaaagatact tataaagaat atattcgtaa caatttagct tttgaagctg
    31561 gcttgaaagc tcacgtagat attacagatg acgatttgaa aactgcttgg aaatctttcc
    31621 atccagaagt agaagcacaa atcatcaaat tatcaagtga agatgaagca aaagatgtga
    31681 aaaaatcagc ggatgatgga gatgatttct ctaaattagc caaagacaaa tcaactgata
    31741 cagagacaaa agaagatggc ggtaaagtta aattcgactc aacgacaaca actattcctg
    31801 cagaagtaaa agaagcagct ttcaaattga aagacggtga gatctccgat gttattacta
    31861 caacaaatcc tacttcttat gcgacagaat actacgttgt caaaatggtg aaaaaccaaa
    31921 ataaaggcaa tgacatggat aaatacaaag atcaactaaa agatatcgct actgaaacta
    31981 aactaagtga caatgcattc acaactaaag taatcggtga agaattaaaa gatgcaaatg
    32041 taaaaatcaa agatgatgcc tttgaaaatg tgttgagtgc attcacaaca acttcatcaa
    32101 gtacgaaaga ctcaagtgaa actacagctt caacaaagag ttctgacaca aaatcaactg
    32161 attcaacaaa agaatcttca actgaagaaa caactgattc atcaaaataa a
//
LOCUS       DAZAJZ010000025        31897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000025 DAZAJZ010000000
VERSION     DAZAJZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.025"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG86D_001115"
     CDS             3..371
                     /locus_tag="ACG86D_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN3140273.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG86D_001116"
     CDS             551..1492
                     /locus_tag="ACG86D_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN3140274.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG86D_001117"
     CDS             1561..2292
                     /locus_tag="ACG86D_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3140275.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG86D_001118"
     CDS             2436..4616
                     /locus_tag="ACG86D_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN3140276.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG86D_001119"
     CDS             4636..5118
                     /locus_tag="ACG86D_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN3140277.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG86D_001120"
     CDS             5134..5895
                     /locus_tag="ACG86D_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN3140278.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG86D_001121"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG86D_001121"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN3140279.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG86D_001122"
     CDS             7667..8152
                     /locus_tag="ACG86D_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN3140280.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG86D_001123"
     CDS             8145..8828
                     /locus_tag="ACG86D_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN3140281.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG86D_001124"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG86D_001124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN3140282.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG86D_001125"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG86D_001125"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN3140283.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG86D_001126"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG86D_001126"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN3140284.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG86D_001127"
     CDS             13125..13457
                     /locus_tag="ACG86D_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN3140285.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG86D_001128"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG86D_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN3140286.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG86D_001129"
     CDS             14283..15656
                     /locus_tag="ACG86D_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN3140287.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG86D_001130"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG86D_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN3140288.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG86D_001131"
     CDS             16711..17151
                     /locus_tag="ACG86D_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN3140289.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG86D_001132"
     CDS             17295..17996
                     /locus_tag="ACG86D_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN3140290.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG86D_001133"
     CDS             17993..19114
                     /locus_tag="ACG86D_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN3140291.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG86D_001134"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG86D_001134"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN3140292.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG86D_001135"
     CDS             20493..21401
                     /locus_tag="ACG86D_001135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3140293.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG86D_001136"
     CDS             21436..21708
                     /locus_tag="ACG86D_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN3140294.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG86D_001137"
     CDS             21719..22765
                     /locus_tag="ACG86D_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN3140295.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACG86D_001138"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACG86D_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN3140296.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACG86D_001139"
     CDS             complement(25776..26462)
                     /locus_tag="ACG86D_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140297.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACG86D_001140"
     CDS             complement(26440..26934)
                     /locus_tag="ACG86D_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN3140298.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACG86D_001141"
     CDS             complement(26954..28174)
                     /locus_tag="ACG86D_001141"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3140299.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACG86D_001142"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACG86D_001142"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN3140300.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="ACG86D_001143"
     CDS             30352..31263
                     /locus_tag="ACG86D_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140301.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        10772 a         5569 c         6853 g         8703 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataag
//
LOCUS       DAZAJZ010000026        30899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000026 DAZAJZ010000000
VERSION     DAZAJZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.026"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG86D_001144"
     CDS             complement(296..1024)
                     /locus_tag="ACG86D_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140302.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG86D_001145"
     CDS             complement(1002..2576)
                     /locus_tag="ACG86D_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3140303.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG86D_001146"
     CDS             2805..3743
                     /locus_tag="ACG86D_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140304.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG86D_001147"
     CDS             3755..4666
                     /locus_tag="ACG86D_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140305.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG86D_001148"
     CDS             4690..6162
                     /locus_tag="ACG86D_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3140306.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG86D_001149"
     CDS             6213..6824
                     /locus_tag="ACG86D_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3140307.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG86D_001150"
     CDS             6854..8767
                     /locus_tag="ACG86D_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN3140308.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG86D_001151"
     CDS             8895..11876
                     /locus_tag="ACG86D_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN3140309.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG86D_001152"
     CDS             11873..13534
                     /locus_tag="ACG86D_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN3140310.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG86D_001153"
     CDS             13574..14506
                     /locus_tag="ACG86D_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN3140311.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACG86D_001154"
     CDS             complement(14898..16445)
                     /locus_tag="ACG86D_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN3140312.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG86D_001155"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG86D_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN3140313.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACG86D_001156"
     CDS             complement(16890..17084)
                     /locus_tag="ACG86D_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140314.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACG86D_001157"
     CDS             complement(17310..18512)
                     /locus_tag="ACG86D_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3140315.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACG86D_001158"
     CDS             complement(18515..18709)
                     /locus_tag="ACG86D_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3140316.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACG86D_001159"
     CDS             complement(18756..19604)
                     /locus_tag="ACG86D_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN3140317.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACG86D_001160"
     CDS             complement(19809..20159)
                     /locus_tag="ACG86D_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140318.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACG86D_001161"
     CDS             20337..21200
                     /locus_tag="ACG86D_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3140319.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACG86D_001162"
     CDS             21824..22816
                     /locus_tag="ACG86D_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140320.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACG86D_001163"
     CDS             22832..23716
                     /locus_tag="ACG86D_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140321.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACG86D_001164"
     CDS             23765..25372
                     /locus_tag="ACG86D_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140322.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACG86D_001165"
     CDS             25373..26857
                     /locus_tag="ACG86D_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN3140323.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACG86D_001166"
     CDS             27034..28026
                     /locus_tag="ACG86D_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3140324.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACG86D_001167"
     CDS             28078..28947
                     /locus_tag="ACG86D_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3140325.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACG86D_001168"
     CDS             complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACG86D_001168"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN3140326.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         9690 a         5145 c         6234 g         9830 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt
    29161 actaaaaatc tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa
    29221 gcaaagccct tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac
    29281 catcttattt tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta
    29341 ttcctcatcc atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg
    29401 gtaatatcat acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt
    29461 ttttgcaaga cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg
    29521 acagcacgga tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag
    29581 cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg
    29641 atttcttcgc gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat
    29701 tcaccaagga cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg
    29761 tcaggcatgc ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat
    29821 ggctcgatca gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg
    29881 atggtttggg cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca
    29941 aggaaagaaa cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca
    30001 aattcatttc caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat
    30061 aagaaacgtt ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt
    30121 tccataactt gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt
    30181 aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct
    30241 gaaaggccta ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg
    30301 tcgatgaagt tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg
    30361 cgcaacaagt catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac
    30421 attttgcgtt ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca
    30481 tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt
    30541 tcaggagtgt tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg
    30601 tattcacggt ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata
    30661 ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag
    30721 gccttctcat cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt
    30781 gcctttactt cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca
    30841 cgaatacggc gagtgatcag ttggttatac tgactaccgt aatcaagtac gataatttt
//
LOCUS       DAZAJZ010000027        29138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000027 DAZAJZ010000000
VERSION     DAZAJZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.027"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG86D_001169"
     CDS             complement(<1..1031)
                     /locus_tag="ACG86D_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3140327.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG86D_001170"
     CDS             complement(1113..1802)
                     /locus_tag="ACG86D_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3140328.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG86D_001171"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG86D_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN3140329.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG86D_001172"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG86D_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN3140330.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG86D_001173"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG86D_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN3140331.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG86D_001174"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG86D_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN3140332.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG86D_001175"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG86D_001175"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN3140333.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG86D_001176"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG86D_001176"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN3140334.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG86D_001177"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG86D_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG86D_001178"
     CDS             complement(7636..8523)
                     /locus_tag="ACG86D_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3140335.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG86D_001179"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG86D_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN3140336.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG86D_001180"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG86D_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN3140337.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG86D_001181"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG86D_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3140338.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG86D_001182"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG86D_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN3140339.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG86D_001183"
     CDS             complement(10454..10957)
                     /locus_tag="ACG86D_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN3140340.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACG86D_001184"
     CDS             11554..12519
                     /locus_tag="ACG86D_001184"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3140341.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACG86D_001185"
     CDS             complement(12618..13790)
                     /locus_tag="ACG86D_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140342.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACG86D_001186"
     CDS             complement(13777..14922)
                     /locus_tag="ACG86D_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140343.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACG86D_001187"
     CDS             complement(14936..15868)
                     /locus_tag="ACG86D_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140344.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACG86D_001188"
     CDS             15985..17064
                     /locus_tag="ACG86D_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3140345.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACG86D_001189"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACG86D_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACG86D_001190"
     CDS             complement(17789..18016)
                     /locus_tag="ACG86D_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3140346.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACG86D_001191"
     CDS             complement(18119..20707)
                     /locus_tag="ACG86D_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN3140347.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACG86D_001192"
     CDS             complement(20727..21386)
                     /locus_tag="ACG86D_001192"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3140348.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG86D_001193"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG86D_001193"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN3140349.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACG86D_001194"
     CDS             complement(22159..23025)
                     /locus_tag="ACG86D_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN3140350.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACG86D_001195"
     CDS             23134..23775
                     /locus_tag="ACG86D_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN3140351.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG86D_001196"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG86D_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN3140352.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACG86D_001197"
     CDS             complement(25216..26115)
                     /locus_tag="ACG86D_001197"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3140353.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACG86D_001198"
     CDS             complement(26117..26914)
                     /locus_tag="ACG86D_001198"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN3140354.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACG86D_001199"
     CDS             complement(26892..27596)
                     /locus_tag="ACG86D_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN3140355.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACG86D_001200"
     CDS             27727..28302
                     /locus_tag="ACG86D_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN3140356.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACG86D_001201"
     CDS             28420..29097
                     /locus_tag="ACG86D_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN3140357.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8576 a         6170 c         5239 g         9153 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aatgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    29101 aggctctttg tcaaatgatg ttggtaaaga gaaatctt
//
LOCUS       DAZAJZ010000028        27797 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000028 DAZAJZ010000000
VERSION     DAZAJZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.028"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..1199)
                     /locus_tag="ACG86D_001202"
     CDS             complement(255..1199)
                     /locus_tag="ACG86D_001202"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN3140358.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1495..2439)
                     /locus_tag="ACG86D_001203"
     CDS             complement(1495..2439)
                     /locus_tag="ACG86D_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN3140359.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2660..3874
                     /locus_tag="ACG86D_001204"
     CDS             2660..3874
                     /locus_tag="ACG86D_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3140360.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3896..5911
                     /locus_tag="ACG86D_001205"
     CDS             3896..5911
                     /locus_tag="ACG86D_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN3140361.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5994..7367)
                     /locus_tag="ACG86D_001206"
     CDS             complement(5994..7367)
                     /locus_tag="ACG86D_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN3140362.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7393..7677)
                     /locus_tag="ACG86D_001207"
     CDS             complement(7393..7677)
                     /locus_tag="ACG86D_001207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3140363.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7679..9694)
                     /locus_tag="ACG86D_001208"
     CDS             complement(7679..9694)
                     /locus_tag="ACG86D_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3140364.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9873..10178)
                     /locus_tag="ACG86D_001209"
     CDS             complement(9873..10178)
                     /locus_tag="ACG86D_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN3140365.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10267..11298)
                     /locus_tag="ACG86D_001210"
     CDS             complement(10267..11298)
                     /locus_tag="ACG86D_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3140366.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11505..12380
                     /locus_tag="ACG86D_001211"
     CDS             11505..12380
                     /locus_tag="ACG86D_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3140367.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12532..13374)
                     /locus_tag="ACG86D_001212"
     CDS             complement(12532..13374)
                     /locus_tag="ACG86D_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3140368.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13466..15343)
                     /locus_tag="ACG86D_001213"
     CDS             complement(13466..15343)
                     /locus_tag="ACG86D_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3140369.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15340..16761)
                     /locus_tag="ACG86D_001214"
     CDS             complement(15340..16761)
                     /locus_tag="ACG86D_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3140370.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16764..20153)
                     /locus_tag="ACG86D_001215"
     CDS             complement(16764..20153)
                     /locus_tag="ACG86D_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3140371.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20410..21801
                     /locus_tag="ACG86D_001216"
     CDS             20410..21801
                     /locus_tag="ACG86D_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140372.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21874..22521)
                     /locus_tag="ACG86D_001217"
     CDS             complement(21874..22521)
                     /locus_tag="ACG86D_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3140373.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22529..23215)
                     /locus_tag="ACG86D_001218"
     CDS             complement(22529..23215)
                     /locus_tag="ACG86D_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140374.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23221..24294)
                     /locus_tag="ACG86D_001219"
     CDS             complement(23221..24294)
                     /locus_tag="ACG86D_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140375.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24516..25190
                     /locus_tag="ACG86D_001220"
     CDS             24516..25190
                     /locus_tag="ACG86D_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3140376.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25195..26535
                     /locus_tag="ACG86D_001221"
     CDS             25195..26535
                     /locus_tag="ACG86D_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN3140377.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26684..27670)
                     /locus_tag="ACG86D_001222"
     CDS             complement(26684..27670)
                     /locus_tag="ACG86D_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN3140378.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8105 a         5248 c         5044 g         9400 t
ORIGIN      
        1 attagatatg aatcattgta tttgccgttg cggaaggatt ttttaattaa ccacagataa
       61 acattgataa cgattcgctt attgtaatca acagattaaa tttcatatgt tcatatgtac
      121 ggatacattt tcggaccgat taattttaac cattctattt atttgcttac ctattagata
      181 tcaataaaaa actgcttaac agctatttca gctgttaagc agttttagga tttttagttc
      241 atattcattg tttcttataa tacagtgttc aatacttctc ttaaagcact tgttgatttg
      301 ctgaacattg ctttttcttc ttcgttgatt gataattcaa caacttcacg aacaccattt
      361 tcgtcaacga ttgatggcac acctgtaaag atatcttttt cgtcgtattc accagttaaa
      421 taagcagata caggcaatac agcttgttcg ttattcaaga tagcttttac gatacgtgca
      481 gtactcatac caataccata gtaagttgct tttttacgat caatgatctc ataagcagca
      541 ttacggactt tatcagcaat cacatctaat tcatcttggg caatacgatg gtctttttct
      601 acaatttcaa acactggttt accaccgaca gttgtatgtg accatgcagc aacttctgaa
      661 tcaccatgtt cacctaagat atagccgtgg acgctgcgtg ggtcaacttt taatttcaat
      721 gcgatttctt tacggaaacg agttgtatcc aatgttgttc cagtaccgat aacacgtgat
      781 gttggcagac cagattcgtt ccaagcgata tatgtcaaaa tatctactgg gtttgaagca
      841 acaacgataa taccgtcgaa tcctgatccc atgatttctt ttacgatttg gcgcatgatt
      901 gacgcatttg ttttaactaa atctaaacga gattgaccag gtttttggtt gataccagct
      961 gtgataacaa cgatatcagc atctttacat tcttcatagc cacctgacca tacagccaca
     1021 ttttcatcgc cccaagccat accatctaaa aggtccaatg cttccccttc tgctttttct
     1081 tgattcacat cgatcaatac taattcatta gagattcctt ggttgatcat tgcataagcg
     1141 atacttgttc caacaaaacc tgtaccgacg attactactt tacgacttgt ttttttcatg
     1201 aataaacagc tccttttcat aactatttgt tactttcatg aaaagtatag tcttgtttat
     1261 tcattttatc aacatgttaa ttataatagt tgttattata atttttgatg gaaacgaatt
     1321 cctgaatacg cataacagat aaatgacaaa tgtttacacc tatatcccct gataatacaa
     1381 ccaaaagttt agaactgaaa ataaatccaa aaaaattcgc tgcaacgata taataagaca
     1441 gcaatcgcac ctaaaacaat tttccttcat atgctttagt gaaagatttt cttcttaatg
     1501 tttatttctc cattcattgg gagtacatgt tgaatacttc ttgaataatt tgaataaaga
     1561 agtcgtttgt ttatatccga tacgattagc gatttcttcc aacgaaaaac tcgtttcttg
     1621 aatcagttcc aacgcaaagt cgaatttttt ctttagaacc agttcttgaa aggaagcatc
     1681 aaaatacatt ttcaatttat tactaagata gaccggattg tagccaaaat gagctgctgt
     1741 ttcttttaac gtacaagaag taaaatgctt atcgatataa tctaatactc taagcatctc
     1801 attttctgcg catccatctg ctgatgaaaa gctttgttgt tctaataaat gagtcaattc
     1861 gatcagtaat agtgatgtat agagatttaa cgaacggatc ttatgactgt atgtatcaaa
     1921 ataaatcaac agcatatttt caatcaaatg cgtcaaacga cttgtttttc cgccgcgaaa
     1981 atgaatgaat tgtgtatgtt cccctttttc ttttgatgca tttatcaaaa aagaagaaag
     2041 tagattattt gtgcttgcca aatgattcaa tatctctgta gataaggttt cttcttttaa
     2101 taagatgttg attaaaatat cctcttgccc tgtgtattcg attctttgag tagcttcttt
     2161 atcaatgatc accacgtcac tttcttccat ctctatcctt tctccattaa taatttttaa
     2221 acatgtaccg ctatacatat aattgatttc aacaaaatca tgtgtatgct cgggaacttc
     2281 cgcatatcga cggtgtttac tgatatgaat atttccctgt tgaaatagat cccctcggtg
     2341 gatttttggc ggtgtctgaa gatggtaagc taagtttaaa tcattgatat tcattccttg
     2401 ttcacgctgc agagtttcga ttgattcttc gttacgcaaa aaaaatgtct aattcagcta
     2461 gttccaattg actccctcct ttttgtaagc ggtttctttc gcttattagt tcacgctgta
     2521 ctgctattat atgcaaagct atttttggtt tcataaagac tttctatggt tgggacatga
     2581 acttcctgtg attccgctat tgagagaata accacttcat actatatttt tgataagcgc
     2641 ttacaaaaat gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc
     2701 agtctctctt attttgactt ctgctggtgc agtcacgtct gcaattccca gtatgtcagc
     2761 tacttatgcc acgcgttctt tatctgctgt tgaattatta acgactgctc cctcttttat
     2821 ggtcactatt tttgttttga ttagtagttt tattgctagg aaaatcggac aaaaacaaac
     2881 tgtgatcatc ggattagtga ttgcatcgat ttgtggtatc ctccccctct tctcacaaag
     2941 ttacagtatt gtcttagctt caagagctgg actaggtatt ggctttggat tgatcaattc
     3001 tttggcagtc agcatgattg ctgcctttta tcacggagat gaacgagcct ctatgattgg
     3061 ttttcaaagt gcatttcaag gatttggtgc agctttaatg acatttgttg caggacagct
     3121 agtacagttc ggttggcaat ataccttttt agtctacgcc atcagcttac ctattcttgt
     3181 cttgtttgtt ttgtttgttc ctacacctga aacaactgaa cttcaatcgc atggcacagc
     3241 cccaacaaga caagtaatca aaacgaatcc taaaatgatt agttatggta tttttctctt
     3301 gctggtcatc gtggtatata atgctgtatc gattaaatta gccacagtcc tgacgactta
     3361 ttctctagga aacgaaacta atgcagcgaa tatcttaaca atcatgcaat tggcaagtat
     3421 ggctgccgga gtttgttttg gctggcttca gacaaaagca catcgctata tgcttacact
     3481 ttctttacta ctgatgggaa tcggctttgt attgattgcc actgtgataa acctgtatgt
     3541 gattgggttt ggtgcgatcc taacaggaat ttctttttcg ttattcattc cttacttatt
     3601 caaccaagta agtatccaag cgcccaagta cgcggagagt ttcaatactt caatcttatt
     3661 ggttggcgct aatttaggta ctttcattgc accatatggt ttgctctttc tctcttttgc
     3721 ttctcttggg agtaaaacac ttgctgtttt cgtcaatggc agtatcttgc tgttgattag
     3781 cgcgcttatt agccttctag tagtacacag gacgaataga aagacagtga taagagtaga
     3841 tgaagaaata aaagaacaat tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac
     3901 aaatgaaaaa cataaagata gatggctttg gtatcctgga gactttgaga tccgccatgg
     3961 actgctacaa aatttccaac gggaagaacg aggttttgac tggccagcgt actggtacat
     4021 ggatgattgc catcggaacg tcaagtttaa gagatattac tttcttgatc aaccttccat
     4081 gtttaaagtg acgatccaag gagttgggta tgtagaaatt aatggacaaa aacatccatg
     4141 tggaaaatgg ctgacttgtc cagctggtaa agcaaaaatt cgtatctttg taggacatac
     4201 atcagggcta ccagcgatgt ttatcgaggg agacgaagta aaatcagata ttggctggac
     4261 agcaagtaat tttattgaag aatatccagc tggctggagt ccgctttatg tcgatatcgc
     4321 aaaagatcca aatcaaatct attatcaaaa agaatgcgtc catccagtca ctgaaacgga
     4381 gattggcgga ggaatcttat tcgattttgg gcgagcagtg aatggctgtg tagaactaat
     4441 atctctggat gaccatatcg aatctatcac tctatgttat ggagagtccg atgtagaagc
     4501 tatggatatc gattattgct actataagca aataggaatg acaagaaaaa tgaaagctcg
     4561 taaacgcgct tttcgctatt tgtttatacc agaggtttct actggaatgg ttcaacttca
     4621 agtttttcat gaatttttat ctctttcgaa acaagctgct ttttcaagcg attcttcttt
     4681 actgaatcag atctggtcag tatcagaaga agccttcaag ttatgtagtg atttgttttt
     4741 tatcgacggt atcaaacgag atcgctggat ttggtctgga gatgcgtatc aatcattcat
     4801 gatcaatcaa tatctttttt ttgacgagga aatcaataaa cgaacgatat tagcattaag
     4861 aggacaaaat gaaatcaagc agcatatgaa tactatcgtt gactattcga tcttgtggtt
     4921 gattggtatc gaaaaccact atatgatgac gaaagactat gaatttttac gcatcgtcta
     4981 ccctaagatg caatcattaa tgagatactt gatggagcaa acgaatgaat tagggtttat
     5041 ttatgggaga gcaaaagatt ggatatttat tgattggtct gaaatggata aagaaggaac
     5101 gcttgctgca gaacaagtct tattactgaa agcctatcat tccatgatca cttgcggaga
     5161 tatcttagct gaaacatatg gagagaacga aaattatcat actcatctct atcaagaaaa
     5221 atatcaacta ttgaaacaaa acctatttga ctacttttgg gatgaagaac ttggcgcttt
     5281 tatcgattgc tatgaatctg ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt
     5341 tttattcgat tttgctaaca aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa
     5401 tgacactatc acacagatta ctactccata tttcaaattc tatgaacaag atgctttatg
     5461 caaactcgga cagacgaata tcgtctacca aacgattctt gattattggg gagggatgtt
     5521 atctcatgat gcggtaactt tctgggaaga atacgaccct actcaaacag gaaatgaaaa
     5581 gtacagtatg tatggagatc cttatggaaa aagcttatgc catgcatggg gagcaagccc
     5641 aatctatctt ttaggacgct attttatcgg tttgtatcca acaaagccag gttatgaaga
     5701 atttatgata caacctaatc tagattcatt tgaaacactg aattgtacgc tacctatcaa
     5761 agatggaaag atttcgcttg aaaaaacaaa agataagatc gtgattactt ctgatagaac
     5821 aggcggaaca gtgaaaatga atggtaaaag ttatcctctt ctagcaaaac agccacttgt
     5881 cttaacaagc tattcaaaaa taaatgcata aaacaaaaaa ctgcttgcga catatcagaa
     5941 gactttaaat cttcttctga tattgtccaa gcaattttta ttttgaagtt tgcttaattc
     6001 tgttctttag aaacaatgtt tgttcttttt ccggcaaaaa gaaagctaga taatacgaca
     6061 agtaaaaaga aagcgatgat tccaactgtc aaggctattt gaccgtattc tcctagtttc
     6121 ccaagtacaa gtcctggtac tagataatca gtatcggcaa atgtagaaga agcgatttta
     6181 agatctccta atactggaag gataaataat ggcagccaac taatcaataa gctattgaaa
     6241 gcagaaccta ataccgcccc tcttctaccg ccagtcgagt tgccgaagac accagaagca
     6301 gctccgcaga agaaatgtcc tactacccca ggaatgataa ctgtagtttg caagcctatc
     6361 atagcaaaca tggatagtgt tcctacaaag aagctaacaa aaaagccaat taatacagca
     6421 ttaggtgcat atggaaaaac gataggaaca tctaatgctg gtttagaatc gggtacgagt
     6481 tttttagcaa tcccttggaa tgctggaacg atttctgcca acaccattcg aaccccttga
     6541 aggacgataa caaacccagc tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg
     6601 ttgtctccat tgcttagttc tctttcaaca aatccaggac cagctaacaa agcaagtgcg
     6661 acatatacga tcgacatcaa gagcatgatc gatacagtag aatctcgcaa aaagctgaaa
     6721 cttttaggaa tctttatgtc ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc
     6781 catccactag aagcataacc gatatttccg gtatgcccta acgcaacttg attgtttcca
     6841 gtgatgttct tcataaacgg ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg
     6901 aagaccccgc ctaataagat catccacaaa tttgcagtaa accccgcgct tactaaaatg
     6961 actgctgtta gacaagaaac ataaagcatt gcttgacctg taagaaaaat gaatttatat
     7021 cttgtgaatc tagcaagtaa aatattcacg atcatcccta ctagcatgat caaagcagta
     7081 ggtgtaccgt attgttgcaa agcaatcgcc actactgctt cattgctagg aactactcct
     7141 tgagtatgca gagctgtttg gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa
     7201 ccagcaccgc cgatcagtac taggaatccg acaaatgttt tgatagttcc ttggatcact
     7261 tctgaaaatt tcttcttctg aagaacgaga ccaagcagtg caatcaaaga aacgaggatg
     7321 gagggctgac tgacgattga cacaaacaaa ttcaacaatt tatccattga tagttcctcc
     7381 cttgaatcat ttttattctt ttgacaagat cttcgttact ttttctttta cttcttcgct
     7441 gtcaatgatc gattgcaaag gtacgagctt ttcatcaggt atagaagtta gcgtatcttt
     7501 caaagaatgc tcaatgaaga aataatcagc catatcgcta gatacacttc caacatccgt
     7561 atgacttact tcaaactcgt ccacatctgc tccaagatct gcaagtgctt gtttgatgtt
     7621 catctctacc ataaaacttg ttcctaatcc actttgacat actgcaaaaa ttttcatatc
     7681 attcgtctcc ttttcttatg atcgctaata tttcttcttt tgttgatgcg tgtaataaat
     7741 cttgaagatt ttgtttatta gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg
     7801 cctcattatc gatagcagct aaacagatga acaaactaat gggatgggct ggattatcaa
     7861 gcagatccac aggttgttga agtttcagca aagacatccc tacttcgttt acgccatctt
     7921 ctggacgagc atgaggtaat gcgatgtgat cgccaatatg gataaacgca ccatactgct
     7981 ccacgcgctc gatcatagct ttgatataac tttcttctat tttattttct gataatagcg
     8041 gctgtgcggc aaatgtgatt gcttcttgcc agttttttgg ttgatccgat aattggatca
     8101 tttcttctgt taataattca gatagcatag gacgttgatc ctccttcttt tctaaaagtt
     8161 tgttcatttt acgatttaat acttgataga gcttttcttc gtctacgcct tcttttaatt
     8221 cgatataagg cagtagcgcc gacaacaatt cttgggcaga aggcattgaa aatcctggta
     8281 tcagccaatc tctttgtact tgattgatca gctgattttt ttccaagtca gtcatgattg
     8341 gatgcagaac atagagagac tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa
     8401 taatatcgta agaagtttcg tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa
     8461 aaaagatcat cgggaataat tgctgaagtt cactttttaa gatcaatgaa gagctaatgc
     8521 cacttggaca gacgatcacg gctcttattt ttctatttct ttcttctgcg tccgcttttc
     8581 tgatctctcc gccaaatagt atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc
     8641 ctattcgtct ttctaacgga agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt
     8701 gatgcttaat ttgatcgatc atcacattag gtaaataaaa agaataggaa atacgaaaat
     8761 aggcagggac caagtgataa aagacattca tcaataattc acgaaacgaa gcaaattcta
     8821 cggccatcag gaattccatg cgatggatga cttctgaaga acattccagc aaaaaatcaa
     8881 gtgcaggctc ctggacatct ccttcaacga tcgatgctaa gcaaagagag aaaaatataa
     8941 tctccgaatt ttgatgttcg gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag
     9001 tgactgcaag agaagccaac agttcagcat cttttaccag ttccatggat gtttttccaa
     9061 ttcgaatggt tgtcaaactc aaataataag ctaattcatc cattcgattt ggtgcgaatc
     9121 gaagatctga tagtgaaatc gttttatcta gcaaatctct aactttggcg taataatcaa
     9181 tggaaagggt tgcggttaaa tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac
     9241 gagcagtgcg attttttgct tctcttctta atattgcttc tgaccctagc agcctaaaac
     9301 cagttttcct agtatacgac agctgacaat cttccacttc taattcattt cttatctttt
     9361 tgatatctga caggactgtc cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag
     9421 aaacagacgt tgcagtcgaa taaaccatca aataaagaag atctcttcgc tcttgttcgg
     9481 tatacacagc ttcaaatcga gtaatatcca aaaatttttc tgcccagtcg attgctcgga
     9541 atttctttat acggatacgg gtatcatcca catattcggc aaaaggagtt aattgtttga
     9601 tcgcctcgct tgtatttact tcgatgatct ctgctgcttt ttctagactg atttctcttg
     9661 gtgttttcag gagataacta attttgtagt tcacagcgct gttcacctcc tacttatatt
     9721 ctagtatggt agcgctttta aatcgagggg tgtttggtac aacttacagt tggatttttt
     9781 ttacgactgt accgaacgat ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat
     9841 gtctcctgct tttatgaatt gatttcgtcc gatcaatgat gatcatgttt attttttgag
     9901 gtcagttctt caaatttttc tttgacatct tcaaccgctt tttcgatatc tttttcaaag
     9961 tgattgggct cattaggctt tgtttcctct tctacagaat ttgaaccaga taaagctgtt
    10021 tctttatttt ctgtttcacc agagtttgtg ccagctaatg attcttcttt gtcttctgtt
    10081 tcaccagaat tagttcctgc taaagccact tcttcttctg ttttaggggc cattgaacct
    10141 cttaatcgtt tgtgtttttc accgtcataa tttgtcatgt tggttcctcc tagtagttct
    10201 atactttttc atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc gattcttaca
    10261 aatatgtcat atttttcctt ctgattcttg aatgcttttg attccttctt ccaaaatttt
    10321 catttgtaag attgtttctt catctttgat gactttttct gcgccattaa ctaatgtctc
    10381 gtaaatccct tcgtagacac ggctgtaatc accaacctcg gaaattactt tttcttcatg
    10441 ataactccct tgttcatcta tataggtcaa tacaccataa tgttcgggca aatcgatacc
    10501 aaagtcttca tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt
    10561 ttgtttcacg aacatccctt tttttccgta aacgacgaaa cttggacgtt ctttgatgcg
    10621 gaaataactg gattttatcg aaattttcat atttccatag aaaaaatcca gatcgaagta
    10681 atcattcatt cgtcctttcc ctaaaagttg acgtacatca tagctgatat catctggttc
    10741 gccgaaataa gaaagaactt gatctactgt atgacaagca tgtccatata aatagctgtt
    10801 cgcagtagaa aattcatgcg tattttctgg tacttccggt ctgaaataat cataatgcat
    10861 ttctacttct aaaatatccc ctaattttcc gctttcgatt actttttgga ctgtcaagaa
    10921 gtcagaatcg aaccgtctgt tttggtaaca ttggataaat agaccttttt cttttgctaa
    10981 tgcaaataac tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt
    11041 cttaccatgt tcgagtgcca tttttccata ttcataatga aattgactag gtgtagtgat
    11101 gacaactaat tggatctcag gatcatccca aagatcagac agctgatcag tatattcgat
    11161 gtcttcgaat gttttccaca ctggttcaga tggggaaaaa atttttttga tattgatttt
    11221 gttcgtaagt tttcttgaaa aaggcaaatg atagcgattt gtactctttc cattgccaag
    11281 ataagcgatt gtaagcatga gtggtgcctc cttttattga tgttttcagt atatcctaga
    11341 aaagcgcaga aagtatttat aatagctatt ctggtaaaaa agttcagaaa aacacttgtt
    11401 atcattcggt ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa agatagtagc
    11461 ggaaccaagt gtttatcagt aagatagaat ttggaggtca gatcatgctt attgaagaaa
    11521 aactaacaaa acaagagttg tttaccacga cagagaaacg gattgtcgat tacatccgta
    11581 ggaatatcga agctgcagtc tacatgacga ttgaagaatt agcaaaagct acgtatactt
    11641 cccattcagc tatcattcgt ttatgcaaaa aaatgggatt cagcggattt aaagaattcc
    11701 gtttcgagct ggggcgggaa gttcatcaat tgattgctca gttcaatcaa acgattgatc
    11761 ctaattttcc tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa atggcggatt
    11821 tatcgatcca gtcaataaaa aaagcacaat tgcagattga acatgaagat ttagatcaaa
    11881 tcggtaaagt attgaacaag gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa
    11941 tcacggcaag gaaattccag aataaaatgg tcaagttgaa taagtttttg atcatggctg
    12001 aagaatacag tgattcttct tggaatgcag ccaatttgac tagcgaggat tgcgccattt
    12061 ttattagcta cagcggtcgc atccatcatt atgaacgaat aatgacctat ttgaagcatg
    12121 tagaggcccc taccttgctg attaccggaa atcagcattc agaaatggcc aaacaggctg
    12181 atatgtgtct tgttatatcg caggaggaat atgattttgc taaagtagcg actttctcct
    12241 ctcaaattgc cttcgattac gtattaaata cgttattctc agtgatatat tctcaaaatt
    12301 ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta
    12361 aagaatatcg gaaaacttaa gaaaagatcc tcatttagta ataagaaaga tagtgtttga
    12421 taggctctct ttgatatcat cataaaaaca ggctgaagtt ttctttttca aacctcagcc
    12481 tgtttgttta cggaacgatt cgaatagata tattatagaa atgaggaata gctaacttct
    12541 gtttcttatt ttgaacgatt gacgaaaaag atatataaga aggaggaaaa tcagtataca
    12601 tgcagctaac aatccataca ggaaaaatcg tttgttctcg gatactttct gtacttcttt
    12661 ttgaacttct tttgcatgat aaggaacccg gtgccctctg acaagtaagc gatgactgtt
    12721 gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt cgattctcag
    12781 atcttttgta tcagtcggtt ctaccgtctt gatctgatcg acttgataag cgagtgtccg
    12841 tccattgatt tcgatataga aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa
    12901 ctttgcttga ggcagtcctc gatggctgga aataacggca tgtgtatttt ctccccccgt
    12961 aggataagac gttccttcca ataatgaggc acctttttct agcagcacag tattcgtttc
    13021 atcaaaaatt ggcaaatgga cattgatctc agggatcgtc aaaataccga tcgtatgttt
    13081 ctcaaaatag gttctatcct ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc
    13141 tggatttcca ccttcttttg ctaattgctg gtttttcttt gtcatgtttt cttggatttt
    13201 agccatgact tcttcatttt ctttgaccgc ttgttgctga taatgagaga tgatctgctg
    13261 atccagataa ttattcagcg cgtcgctaac aaatggataa gctagtatcc ctattcctga
    13321 gagaagcaag aaaagcatca gcagatctag aagtttcctt ttctttggtt tcatttgtgt
    13381 tctccttgtg atgactgttt gataataaaa gaaaaaggag catttgcgta atccgctgct
    13441 cctttttcca attcttgcaa aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca
    13501 gtccagcaat cagtagaagg actactcctg caccgatata cacatagatt cccttaccgc
    13561 ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc
    13621 ctgctgttac ataagattgt tcatcgatag taaatgctac acggtttgtt aatggaacat
    13681 atttttctgg cgctttcgtt tcttccagat agtacgtgcc atattttaga cctgtcacat
    13741 cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc
    13801 tgacggcttt tgtggatggg tcgatcgata aatatttcgc tgtgtcacta tcttgatcac
    13861 gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca tctactttaa
    13921 caaatcgttt gcccccagta acgacatcga ctgacggtgg tgtttgatca tttgtatgcc
    13981 cgttatcgac atttgcttgg ttagaaaatc ctttggttgg atctgctttt tcgttcaaat
    14041 gcatatagta aacgaatttc aatgtaccgc caggagttaa tgaaggaata taattcggat
    14101 caactgaaac cgtgaatccg tctgtttgct cagtgacaga ataatttact gggtcgattt
    14161 ctttatttcc atcatataag gcataagcat acgttcctga agaatcatta tcaaatgtta
    14221 aagcagcgtc atgagtatcg ataagtttga atgttgtgta cttgttttgc gttccttctt
    14281 tatcagcgat acctaatggg atattgacag aaatctcata ttgaattttt tcaccgattg
    14341 cgacatcttt tccattcaat tgaggtgttg ttttatcaac tttagacgta tcatttttga
    14401 tggtcttttc tactggtgtt tggctatttg ccaggatctc aaaaggcgtg attgtttgct
    14461 tttcaatcat ttccgcatta tcaggagctt ttacttcttc taaattatat tttccaactt
    14521 ctaaatgatt aacgatcaac tggccttttt caatagatgc ctcggcaaag tcattgttgc
    14581 cgatgtcata agaatgacca gtaatgaaat gtttggcttt ggtttgatca gttgtccaag
    14641 tgtacaatcc atctgtgaca ctttggatgt attttttgac gcttggtgtt ccttcttctt
    14701 tagaaataat aaattctgct ccatttagtg cttcgttttc ggcagtaccg atttttgtaa
    14761 ctttcaacgt tccatcatta ccgactgtat ttttagggta gagatggata gtatctagtt
    14821 cttctgtccc gtatttataa gagccatctg cttgtttgat catctcatat acagggaaag
    14881 ctaaaaccat gtttgcggca gctgacactc cgtcttttgg ttcttctttg atcgtgtaga
    14941 ctgcatcttt cccattttgt tttttaggta aagataaagt gacatttcca tcagcatctg
    15001 tcgttcctga agcaactggt gtaccaggag tcaaagactg gactgcttgt tttgctgcat
    15061 ccacggacgc tcctttatct cgttgcgcat aaaattcttg agtgacgtta taaactgaaa
    15121 atgaaatatc ggctaatcct tggtattgat cgaattcgct catttctttc ccgctgtttt
    15181 ggattaaagg atcgggtaaa tcagtcattt tctttttatg aaggatgact tgagctgctg
    15241 tctcctctgc aaacactttt tttgcgccga agctgtttgt gagtaatggt aaaataagga
    15301 aaaggactcc taacataacg tttatttttt tatgattttt cattttttca ctcctgttct
    15361 ttttttgata aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag
    15421 tgtatttttc gtttcgtttg tttttggaag aaatggacgg ctattttctt ttttattgac
    15481 tactttcaat atactgtccg cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg
    15541 aatttctgtt tcacttaaaa tataaccaga aggtgcttcg atctctttta atcgataact
    15601 tcctgttttc aacccggaaa ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc
    15661 atctgattcg ttctttgtcc acttgtatcc gttggctgtt tgctggaggt aatttcctgc
    15721 tttgttttta ataaggaata ctgcgccagc taatcgttta tctggtttat ttgtctcgat
    15781 tttgacaaat tgcttactcc ctgtatatac agagactttc tttgtactga caccaaaacc
    15841 aaaatccagt gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg
    15901 catctgataa gaaatggtca gtttttttcc agcatatttt tccaacgaag ggatcgaaaa
    15961 taaaacatca aatccgtgag gtttcttgtg tatgacaaag ccttctgtca gcttttctcc
    16021 attcgatgaa atcgttaaac tattaggtaa aaacgttaat gcttcatccg cactatctga
    16081 caattcgaac ttttgatagt caaggatatt tacaggaatt gtcgtatgca aggaataagt
    16141 gattttttca ccaatcgtaa aatcatttct aggctcttct aataccttct caaaaggtgg
    16201 atcagggtag tcgttttctt catttttagg atacaaatgg atagttgaaa gtttttgatt
    16261 gttttgtcca tgtacaggaa gaacaactac catattctct gccttttctt tgacgacttc
    16321 tggtactttg gattcaatga aaagatacgc tttatctctt ttttcttgat ctttagcggc
    16381 cagagaaaaa gaagcgatac catcttcgtt gttaagagtt gtagttgttg cttctgcggt
    16441 aaacataccg gaagacgcac cgttttttgc gatctctgct tgtgcttctt ctaccgtttt
    16501 gcccttttcc cgtagatggt aaaaagaatc tgtgacatca taaacttgga atgtgacacc
    16561 atttaatcct cgatacgttt gtaataaagc tttttcttgt ccgtcatttg gatgattttt
    16621 cggcagttgc ccgtttggga aaagcaattt gtgtaaagta atctgaacca tttctgtttc
    16681 tgtttctgtt gctacctgag taaaagctgg tttaaatagt aacaagaaga gacacatact
    16741 aagccaacca agttttttca tatttagctg ccccctcttt cttttttcaa aaaaagatac
    16801 caaatagaaa acgcacaacc aatcatccct actagataga ttcctaaacg gccaattcct
    16861 cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt
    16921 ttggctcctg gtgacagaac agattcatga tcttgtccat ccacttgaat tgacccatct
    16981 tcgtgtataa ctatagtaac tggctctttt agaccttggt atccttctgg tgtaaaagtt
    17041 tcggtcagcg tataagttcc aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt
    17101 ccatcttttg gcgattctaa gtccatttct ggtccctgca gtctgaattt tgctcctttt
    17161 aatgtctgtc cgttgtcttc ttttttgtgt accgttagat caaacgcttt tagtcgattt
    17221 tgatgagtga gcgtccatac attatttgtc atctcggtat tttttcccgt gtaaatgatt
    17281 tttccatatt tatcgatttg gaattcataa tttcccggcg aagtatcata tccggcagga
    17341 ccagctgttt caacgactcg atatttacca ggtgtgagtg aatcaaaact ggcaagacct
    17401 ttttcattcg ttgtttggct gtcaattggc tgataagtac catttgcttc ttttttctgt
    17461 agggaaaatg ctgctccttt aagatttatc tctttgtccg tcccttgcat cgcatactta
    17521 cgtactgcta caggaacttc aacaaaagga ttttcaatag tcaactggat cgtatcatcg
    17581 gaagtagtga ctgtttttcc atcaatggtt accgtaccat cagaagcgat cttgatttcc
    17641 caagtagtct ttttgcttaa tccatgccct tctggagctt ttgtttctgt cagcgtatag
    17701 gtcatttctt tttgcaattt gacattttct ggaagagaat aggttccgtc gccatgatct
    17761 gttagtaaag tatcaataga atctcctgtt aatttgaaaa cagcgccaat aagatccttt
    17821 tcacctgtag aggaattttt cgttattttc aagtttaacg gtgtgaattg tttagtattt
    17881 ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt
    17941 gtttggtaac tgaatgcttg accttgatta ttgtattgag gtagtgataa tatctcttga
    18001 taactttctc cgctatttgc agataatttg tcaatgtctg cacgttccca cgtatttgtt
    18061 gtatctttag ctggtttaat gattcgaata tatccgtttt tccaagctgc agcatttgtc
    18121 gtatgttccc gttgaatctc aaaagtaact tgatctggac gatcagctag attgttgtca
    18181 aattcttccc ataacttttt gatgtgaaga ctgactccgg gagctttagc agatggtata
    18241 ccgaattcag ctaattcatt ggtatcaatg tttggctgga aagttgtcct gccgttcatt
    18301 tgataccaga aatttggatg gaagtcctca ttttctgttt ggattctcac ttggtaatgg
    18361 atttggattt cttggtcttt tcctaaatag atctgattgc tcttgatggt atttccttct
    18421 atggaaatag atggagtgac cgttgtagga cttgtcccca cactcttgac tgaaagtgta
    18481 ccaggctgat aaatgaatgg ttctgcgatt ggatcattta tttgtccatt gcttacagtt
    18541 gctgagatct gtacagcttt tttggctagg tattcagaga tatctgttgc atgatcagct
    18601 gattcatagt aaagatcgcc tttttcatct gatgaaacca tttgtcgcat acgagactct
    18661 acttctgctt ttgagagacc agctgccgga tcgctttgaa gttggatgcc aagaccatgt
    18721 atttcgattc ctcgttcttt gagtgccatc gcttctccga tggttgcgat aaacgtactg
    18781 tcgattcttc tggatagatt gttttggtca ggtgcatcat agattcttga gattagagaa
    18841 gtatttcccg gccgatcttg cgtattagaa aactgagttc cgtaataatt gctgcttgat
    18901 tgtgcgtgta cccgctgtac tttataggaa aatgttggta caccatccgt cagcaaaacg
    18961 atcacttttt tatgtccatt tggaacggat agcatgcttc ctgcatctct tagtgctttt
    19021 tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg attttacttg atttttcact
    19081 gaatcaaatg aacccatctg tactgcaccg ttactgtagc tatatccttc gcttgagtag
    19141 ccgacatatc ccatattgat tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa
    19201 cgatcgacac caatctttac ttcaccgatc cgattattgt cgttcatact tcctgaccag
    19261 tctacgacca atacgagatc aagaggcgtg atatcttttt gtacatttcc acgagcattc
    19321 aaataaacat caaacaatcc gggtgtactt gtttcctttg catactttcg tatcgcatag
    19381 tctgcttgat taccggttcc tccatattcg atatacgaat gggtccgatc attaggatct
    19441 ccattccaag aatttatacc atcccattga tttgttccgt ttttattgcc ttgatgatca
    19501 agaacatttg tatttcctgt aggttgccaa ctggctttag gataagtgcc ctgatcatct
    19561 gttgtatatt ctgtaggaat cgtttgatta gatggcacac tcacagctgg caaggataat
    19621 gtaggcaatg taaatgggct gacagaattt tcgttttctt ttgatgattc ttcttctatt
    19681 tcgtctggct ttggtactga cacatctgta tctttcttat tttctgtctc atcaataatg
    19741 tcatattctt tattatcgac gatcagcttg tttgcctggc tgtggtaaag tgctggatcg
    19801 attggaaaac tcagatcgat agtttgttta gtgtttgatg agatttccaa cgtaattttc
    19861 ccgctattta cctgataagc atagccatct ccttcttttc tttccacatc acttaagtaa
    19921 ttttcgacta gaccaatttc tttttctatc ttttcagcac tggtgttatt cgcatttagc
    19981 ttgatgttca gtgctttttt tccagactga tccgaaatga cttcagctga aattgtttct
    20041 aaaactgttg gatcatctgt agtgtctgct attgcagata atggcacaaa gtttgataaa
    20101 ctcaatatta tcagcatgaa aataactttc agtttcttcc ctgtggtcgt catttttccc
    20161 ctcctttttc tttttttctg ttttgacgtg aatagtgaaa attattaaaa taaacaggcg
    20221 ttacataatt ttatttattt taatatgata attattatac gaagggcgct aaagggaatc
    20281 acaccaattt tcataccaat tggtttcatt tatgaagttg attccttcga ttaatgagcg
    20341 tttcattgaa agaaaagggt tgagggagaa aaacatatga tcaggttgtt ggaaggaaac
    20401 tatagaaaga tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg gatgactaaa
    20461 aaagaacttg ccaaacgaat tggtagttca gaatctacat ttgtaaggta tatcgaagag
    20521 atcaaaatcc gctggggaac ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt
    20581 cttgaacatt tgaatgtacc agcttattta aatgttctga tggaaatgtc gcaaagttct
    20641 acaaacagtc gtttattaaa tgagattatt gtcaatccgg gtaaaacgat tgagtattat
    20701 tgtgaaaaaa tttccatcag ccggtcttct tttacacgta agttgaaaca atgcaatcaa
    20761 attttagaaa gctatttatt aaaagtgatc gttgaccaag gttttcagtt aactagcctt
    20821 aaaagtgagc tccagttgcg tatctttgtt acctttttct ttttaagtta ctacggaaga
    20881 cgcacgttgc catttccaat cgaaaaaaag aagatcaaag aaattatggt caagaataat
    20941 tgtcagatga atttgtttca ggaagaaagc ccatatgagc aatctttctt tattatgtat
    21001 tttgttgtgc atctcatgag agaatcccaa ggaactccat ttgaatggaa agctggagac
    21061 tcttatgtac agttgataaa tatcgataaa aaagacttct taatcttgaa aaatctctta
    21121 ggaaaattga agtacagcag ctatataaaa gtagtgaact attttgtcaa tacgattttt
    21181 tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc
    21241 tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta
    21301 ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg atacgactca tttgacgatc
    21361 cgtgcaactt actttgcaca gcagatgcaa caccgccaga tacgtctata tgctggattt
    21421 cgtgcagaac ttcaatttta ttccactcta ctgaatcaga atctgattta ttatttatct
    21481 agctatatct tttggatgct tatcggtaat cctgaaattc accattatac ttctgataaa
    21541 aaaatactga ttatcagcga cttaagtctt cgacatagtc aatatattga agaatatatc
    21601 agtgatattc ttgctgtgca taaaattcac tctgagacaa ctgctattac tgaagatcag
    21661 ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta cgaatcaacc cattttgaat
    21721 gcccatgttc ctcatatatt gattgatgat agtgtgtcat tcgcaaatga agaagaactc
    21781 atccgcttat tcgagttatg aataatcaaa aaacagctat gtataaacca ccctgataaa
    21841 aatttcagga caaatacaca tagctgtttc tttttattgt attccttcag caatccaacg
    21901 agatttatag cgaaagtctt ttaacaactc ttcttttgtc caattatttg caatgagatc
    21961 cgtcaaagtg ttcattacga gggtcatgga aaagtatagg aaactaactg cttcttccga
    22021 cgattgaatg gagaaatgat tgattcttaa taattccagc catttttcgt tcattggaga
    22081 atcagccgat agttctctac gtttggttgt ctgctgatca tttcgctggg ttaacaagta
    22141 taacctttgc caatagatac tttcgtgatc taagtcgcag caccactcta aatatttttc
    22201 gatccccaaa aagaaatgtt gtttgttttc gctaatgaat tttaagatat cttgatggac
    22261 tatgtgagaa ctgcttttga tcgtatacaa ataagcatct tccaaatctt gaaaatattt
    22321 ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt tctgagactt tgacttggct
    22381 gatgtgctgt gagcaaaata tctccagtaa taggtcatcg attctctgtt tctttgcgtt
    22441 cgataaattg aaaaaagttt cttttggcat ccacatctct cctttatctc tgattatacg
    22501 tggatagccc ttcattgtca tgaaggattc attccttctt tttctcttct tctgtcaagt
    22561 acccatcttc cattcgataa acgcgatcac tccattgaat catacggaca tcatgagtaa
    22621 ccataatagt cgctttttgt ttctcatgag cttctttaac tagtaatttg acgacttcat
    22681 atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc taagatcaag cttggctcat
    22741 tatataaagc gcgagcaatt gccactctct gtctttctcc accagaaagg tctttaggat
    22801 aactattttt aagtgactgg atatctaaag attgcagcaa ggaatcgatt tttgttgaat
    22861 cttctttttt tgaagtaact tggtcaacta gttcaaattg ttcctttacc gttaaaaaag
    22921 gaatcaaatt agagctttgg agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt
    22981 ttttttctgg aagctcagtt agtgagtgac cgttgatcac tatttctccg gaagtcggtg
    23041 tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa ggaccaatga
    23101 tgctgacgaa ttctccttcc tttacataaa aatcaattgc tttcaaagct gagacctttg
    23161 tatgaccaga accaaatgtt ttggtaactt ttttcatcgt taatattttt ttcattttta
    23221 ttaacctcct attgctgtaa ttgggtctac tttggttatc gtacgtatcg aaaacaatcc
    23281 gcctagcatt gcgacaatga tcaaaatccc actatatagc aaccattgtg accaaaaaac
    23341 agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca aaagagcaag
    23401 cagaacgcct accaccccaa caataaagga ctgtgctact aaagatttag cgatgaagaa
    23461 atttttgatc ccttgtgctt tcattactcc aaaaatagca gtcttctgaa gtgtcatcac
    23521 gtacatgaaa ataccgacca cggcagcaac cactaagaac aaaaagtata tcatcgcatt
    23581 taaggtcata ttttgggcag aatagccagg aatactttca atgaatgtcc caatagttaa
    23641 taactgtaga cccttcgatg ctttttcttt cgaaatttta gctgaatcct ttattacgat
    23701 cccattgatc ggctgttctt cttcagaagc aaacggctgt tgtccatact tcaaagcagt
    23761 ccacgtatct agggacgcat agatgacggg agaaactgtg taataagttt caggaaagat
    23821 accgacaatc gtcaaaggat cgtcgtaact gccaattttg atcgtatctc caattttata
    23881 gcctgaatca gccagatttt gagagatgat gatttcattc tttgttttaa attcatgccc
    23941 tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg taacattttc
    24001 tttgtttttt cctttgattg ctccgctgta caatccgatt ggtgctttct ctcctccttc
    24061 aatgtatttc agatctcctc ttgttaattg tgaagctgca aatacttgat ttgcatcctc
    24121 cgacaaaaca atctcttgtg cgtcccaatc atcgattgct tttttaaact cttctgaaag
    24181 tccattcgct agtcctgaca acatgaatac gacatacgca atcaagaaca taataccgac
    24241 gatcaagcca tatcgtaatt tagaatagcg aatttctttg atagctagaa acatttgttt
    24301 ttcctcccta tgatagattg atagtgacaa aatgtcactt gttgaatagt gtaactctaa
    24361 agtgacaatt tgtcacttgt gttgtttttt tcttatcaat aaattgtcta agacatcttt
    24421 cgataaaacc ttaacatgag tcataaaaaa tttatttaag ccttatttat gctttccatg
    24481 ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat taaactatta gtagtagaag
    24541 acgataaagt attatccaat aatataaaag aaatactcgc tgcaatcggc gagataacac
    24601 aagtttatga tggtagtgaa ggactgttcg agttggaaag tggagtttat gatcttgcaa
    24661 tcttggattt gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa atacggaaag
    24721 aaaaaataca gactccagta ttgattttga ctgcaaaaga tagtttggaa gataagattg
    24781 aaggatttga acgaggtgca gatgattatc taacaaaacc tttctatcga gaagaactga
    24841 tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa aatgttctga
    24901 agtataagga tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt
    24961 tgcccataca aggaaaagag tttgaattgc tcgtttattt tttacaaaat aagggaatca
    25021 ttttgacaaa agagcagata tttgaccgga tctggggatt tgattctgat acgacgatta
    25081 cagttattga agtttatatg agcaacttac gaaaacatct gcgaagtact ggattggata
    25141 aagaaatcaa aaccttacgt aatgtcggtt atatactgca aagggtgtga tgcaatgaat
    25201 ggagatatta cacgaaagca acgtagtcgc ttttttgcga caaatgtagc agcatttgct
    25261 tttatctttt ttcttttggg attcattact ttacagatat taaaccaatc tgcatacaga
    25321 gaaacagatc tgtcattaga gaaaataaat tttgattctc gaacagtaca attagagata
    25381 caaagatatc agcaaggcga tcccttttta aacccgactc aaacggaaca aaacagaaca
    25441 aacatggaac agccattaat ggaaccatca aatgaccgtt ttaacactca agtgatcttg
    25501 tggtctaaaa atggtgagat attgaataaa gcagctattg gtgggcgttt caaccaaatc
    25561 aaagaattaa aaatggatac gaaaaatctg aataccatcc aaaatattga attaaatgaa
    25621 gacagtggct catctgccct ctctttccgt tcgattacga aaaaagcgga gacgaatcaa
    25681 gatggcgttg cctacatcca aattttagaa aatacaaatc aaatcaagaa ttcattagcg
    25741 acttttcgca cgattatgat tttgtgtatg attgtttttt ggattctctc tattggtatc
    25801 agttattact tgtctagtct aagcatgcgc cctattattc tttcttggag aagacaaaaa
    25861 gaatttgttg agaatgcttc tcatgaattg cgcacaccgt taacgattat tcaaaatagt
    25921 ttggaacatc tttttaccaa gcctaatcat actattattg aagaatccga aagcatcgct
    25981 caagcattgt cagagacgag gcgcctcact ggattgactt cggatttgct gacaatcgct
    26041 cggaatgatt ctgatcagca gctgctttca aagcaaatga tcaatacaca agaatatatc
    26101 gaacaacttg ttaaaccatt tcaagagatg gccatcatag atggaaaaga gtttattctc
    26161 cataacgatg caacattaga agtaccaatt gatgaaaaaa aaatccatca agttttggtt
    26221 attttgctgg ataatgcatt aaaatatacg aatccagaag ataagatcat tttggattca
    26281 tctgttaacc ataaagaatg gctgattcac gtaaagaata ctggcccttc tatcagtgaa
    26341 gaagatcaag caaggatttt tgatcgattt tatcgggaag atcaatctcg ggcgaaagag
    26401 actggcggat acgggcttgg cttggcaatt gcaaaacagg taatcgaaca gcatcatgga
    26461 cagatttctg tatccaatta tcttccacaa ggagtcgatt ttaaagtcca attgccgttg
    26521 aagcctaaaa aataatgctt atccctaaaa aagggctgtg acaaaaatct tgtcacagcc
    26581 cttttttgtt gcgaataaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    26641 tctttaagct gaccacttct gtcacaacct ctttagctta cgcttacgat tttttattgc
    26701 ggtcttcaat gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt
    26761 ctgtcacttt ttcaacatct tcatgttctt ctttatgaat cccaacttct tcacgaataa
    26821 ctgtatgttt gcttacatct acttgctctt ctttgattgg gatagtaatc gtgtcatctt
    26881 ccaatttttc gtctgttccc tgtgaagatt gatctgttac tggtttgcgt tcgatgacga
    26941 tttcttcacg tttgaccggt acttctaccg tttcagtatc gttgaccaca tgtttgtgga
    27001 tgtcgacttc tccagttgtc acatcatgtg tatttacatc taatttttct tcttttagtt
    27061 gaattgtatc atcatctaga tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca
    27121 ttgtatcagt ctcgccaggt acgtccatcg gatcagttcc tggtgtcata ttcaatggat
    27181 ccgttgtatt ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt ccagtgccag
    27241 tttctaatcc agtccccatt gtgcttgctg ggctaacagt atcttgtgta tccattgtat
    27301 cttctgttgt ttctggctga cgatagcctt ttactgtgat gacaaatttt ccatcagaaa
    27361 tatctcctct atattgatat aaaggatcat tttctggtgt agccgtctca gaatcatagg
    27421 tatcaaaaga aaaagtatct tttatttttt cccataaaga acggtcttct tcaccatcca
    27481 tccgattttc atctgtttca acatctatac cagacaatcc gtcaaatcct agtctagatg
    27541 ctgcttcatc agtagtataa ataacgatat catctcgttc atacccttcg ttacgcaacc
    27601 gttcaattac tgataatgct tcttccctcg tactatagct tccgatgacc gttaaatttt
    27661 tctcttccat gtaaattcct cctagcaaat agatttatga ttggtatatt gttacttata
    27721 aatttattct ataggagacg attgttttct acaaatattt agattggaat ttgattaaaa
    27781 caatttaatt gaaattt
//
LOCUS       DAZAJZ010000029        27552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000029 DAZAJZ010000000
VERSION     DAZAJZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.029"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG86D_001223"
     CDS             complement(287..649)
                     /locus_tag="ACG86D_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140379.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG86D_001224"
     CDS             complement(723..1103)
                     /locus_tag="ACG86D_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN3140380.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG86D_001225"
     CDS             1185..2360
                     /locus_tag="ACG86D_001225"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN3140381.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG86D_001226"
     CDS             complement(2437..3021)
                     /locus_tag="ACG86D_001226"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3140382.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG86D_001227"
     CDS             3222..4220
                     /locus_tag="ACG86D_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3140383.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG86D_001228"
     CDS             4332..4841
                     /locus_tag="ACG86D_001228"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN3140384.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACG86D_001229"
     CDS             complement(4871..5530)
                     /locus_tag="ACG86D_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN3140385.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACG86D_001230"
     CDS             complement(5532..6296)
                     /locus_tag="ACG86D_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN3140386.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACG86D_001231"
     CDS             complement(6304..6981)
                     /locus_tag="ACG86D_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN3140387.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACG86D_001232"
     CDS             complement(7018..8403)
                     /locus_tag="ACG86D_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN3140388.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACG86D_001233"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG86D_001233"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG86D_001234"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG86D_001234"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN3140389.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="ACG86D_001235"
     CDS             9341..10198
                     /locus_tag="ACG86D_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140390.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="ACG86D_001236"
     CDS             complement(10279..10614)
                     /locus_tag="ACG86D_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140391.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="ACG86D_001237"
     CDS             complement(10681..12051)
                     /locus_tag="ACG86D_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN3140392.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="ACG86D_001238"
     CDS             complement(12129..12665)
                     /locus_tag="ACG86D_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3140393.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12974..14455
                     /locus_tag="ACG86D_001239"
     CDS             12974..14455
                     /locus_tag="ACG86D_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140394.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14558..15265)
                     /locus_tag="ACG86D_001240"
     CDS             complement(14558..15265)
                     /locus_tag="ACG86D_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN3140395.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15344..15682)
                     /locus_tag="ACG86D_001241"
     CDS             complement(15344..15682)
                     /locus_tag="ACG86D_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN3140396.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15793..16755)
                     /locus_tag="ACG86D_001242"
     CDS             complement(15793..16755)
                     /locus_tag="ACG86D_001242"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN3140397.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16770..18101)
                     /locus_tag="ACG86D_001243"
     CDS             complement(16770..18101)
                     /locus_tag="ACG86D_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN3140398.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18109..18789)
                     /locus_tag="ACG86D_001244"
     CDS             complement(18109..18789)
                     /locus_tag="ACG86D_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN3140399.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19008..19487)
                     /locus_tag="ACG86D_001245"
     CDS             complement(19008..19487)
                     /locus_tag="ACG86D_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN3140400.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19614..21293)
                     /locus_tag="ACG86D_001246"
     CDS             complement(19614..21293)
                     /locus_tag="ACG86D_001246"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN3140401.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21841..23343
                     /locus_tag="ACG86D_001247"
     CDS             21841..23343
                     /locus_tag="ACG86D_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140402.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23356..24018
                     /locus_tag="ACG86D_001248"
     CDS             23356..24018
                     /locus_tag="ACG86D_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140403.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24119..24322)
                     /locus_tag="ACG86D_001249"
     CDS             complement(24119..24322)
                     /locus_tag="ACG86D_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN3140404.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="ACG86D_001250"
     CDS             complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="ACG86D_001250"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN3140405.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="ACG86D_001251"
     CDS             complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="ACG86D_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN3140406.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8192 a         5759 c         4666 g         8935 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga caactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 ctttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa
    12961 aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt
    13021 cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg
    13081 gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga
    13141 tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa
    13201 aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa
    13261 ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga
    13321 acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt
    13381 aataaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga
    13441 gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg
    13501 gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga
    13561 aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa
    13621 caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt
    13681 gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga
    13741 agagacagaa acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca
    13801 aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat
    13861 ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt
    13921 agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact
    13981 gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca
    14041 ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa
    14101 aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca
    14161 aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa
    14221 ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc
    14281 atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac
    14341 ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc
    14401 ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa
    14461 ttcttgtcac atcctcttat tccaaataaa tggtattcaa tcagctaact ctcggtatta
    14521 gctcagatca aagcgctgat ttcacaactt ctttatttta atctcttcgg ttgccgccga
    14581 acaataagat caagcgcaac agctgcagga atgtcgaaag cgctgctgca acataagtca
    14641 gtgctgctgc aaataatacg tgtcttgcca tcggtacttc ttcttccgtc agtagtccgc
    14701 cttcactcaa aatttggatt gctcgccgag acgcgttaaa ttcgactggt aatgtcacca
    14761 attggaaaag taatgctaaa gaaaaagcga aaatcccgat attgattaac gtagagttcc
    14821 agctaagcaa tacacctacc ataatcaagg ggaacgaaat catcgaacca aagttagctg
    14881 ctggaacaag tgctgcacga atcttcaatg ggacataacc cgaagcatct tgtaccgcat
    14941 gtccgcattc atgagctgct acacctatcg cagcaactga agtagactgt gcagttgctt
    15001 gagacaaact caacatctta ttacctgaat tgtagttatc tgtgagatct ccggcaattt
    15061 gttgaacacc gacattattt atctgttgac tttgtaatat atattgtgct gcttgtgtac
    15121 cagtcacatt gtttttgctt ctcacttgat cgtattttcg gaaagtacta ttgacgtacg
    15181 ctgacgcaat tccggaaata actaatccaa gaatgaccaa aagaaatgtc ggatctaaaa
    15241 aaccatacat tccataacca tacattttcg tttcactcct tttcgtttac tttattttat
    15301 cattagtagt aggaaaagaa tgtgaaaaga ccttgaatat tctttaattc tttttcacaa
    15361 attccgattt cagtttcatc ggtccaaatc cttctacctt gcaatcgata ttatgatcgc
    15421 cttctactaa acggatgttc ttgacttttg ttccttgttt aatggcacct gacgcacctt
    15481 ttactttcaa gtctttaatc actgtcacgc tatcgccctc taacagacgg tttccatttg
    15541 catctaatac cactaatccg tcttctatga cagtttcttg ttcttctttg ttccattcgt
    15601 tcccgcattc tgggcaaatc aacaagcctc gatcttcata tgtgtactct gagccacatt
    15661 ctgggcaatt tggtaatgtc atcttctttc tcctttaaaa tctttagtga gcactattgt
    15721 aacggaaaaa gaagatcttg aaaagataca tttgtgattt ttactacaaa aggcacgttt
    15781 tcgaacgaag tatcattctg tttttctgct cttatacgat tggcatatat cttggatctc
    15841 tttatagatt tcctctactt ctgctaaatc ttttgcattc gcaccacttg ctaatggatg
    15901 gcctcctcca tgatggcgtt tagccaattc attgatgatc ggccccttag aacgtaaacg
    15961 tacgcggtaa taccctgaag gctgctgaac aaaaatcccc caagccagca cttcgtcaat
    16021 cactccagga agtgaaacga tcgaagctgt ctctgaatca tcgattccat atttatcaag
    16081 aatttcttgc ggaaggatta ttcttcctgc accattttca tccacctgga tattttgata
    16141 aagataaccg gaaagtttag cgactttcat cggcatttgt tcaatctctc tgttcaaagc
    16201 acttgcatca aatggataat ttctcaatat cgaagcaatc tctaatgtac gggacgtcgt
    16261 ggagggatac aaaaagcgcc ctgtatctcc gacgattcct gcataaagta accgtgcagc
    16321 cttaacgttc atctttaaag attcaggaaa catcaaagca aattctgcaa tcatctcact
    16381 acagctactt gcttttgtat ttacccacac aagatctcca taagggtcat cattgggatg
    16441 atggtcgatt ttgatcagtt tttctgccaa ctgatatctt tgatcactga ttctgggtga
    16501 attagctgta tcagtcacga tgactagcgc tccttggtag aagacatctt caacttcttg
    16561 catttctgct aaaaagcgaa gcccttcaat cgtttcacct acttgacgta tttctttttc
    16621 tgggaaactt tcccgtaata gttcggctaa tcctacttgt gatcctaatg catcaggatc
    16681 agggcgcaaa tgcctgtgga taatgatttt atcgtatttt tttattgtat ctactatttc
    16741 ttgctgtact gtcatttcac tcctgctttc taagaacgtt ccattacttg acaaacaacg
    16801 attgctttgg aaacgatggt gttttcaata aacacttcga tatccagttt agcggaacgt
    16861 ctgccgattt ccaatacacg tggacgaatg tctaattcgc tttccaattg gatcagccgc
    16921 aaataatgta ggttcacttg ttcaatcaag atatttcgtt tttggttcat caccattgtt
    16981 ttttgtgcga catcagaaat gatttcgcta agtacgccaa aagaaatggt tccaacgcca
    17041 ttgaccattt gtggtgctac tacgaatttg aacttagggt tttccaaacg atttccttca
    17101 cggtcagtat caatcgtatg gatatttcca gatatctgat ccgaaatcgt atctgcaatc
    17161 tgcggttgtc gctggacaag ctgcatcgct ttcattacat cttgccgggt aatcaagcct
    17221 tccaatgtca agtcatcgga aacgaccggc atgacttcta gcccatccca gatcatttga
    17281 tggctaacag aggcaatact cattgttttc tttacactga ttggttcttt cgtcatcacc
    17341 cgatcgatga tctggtttgg cgatttccct aaaacatctt ttgccgtaac aatccccatc
    17401 aaacgatgat ggcggttgac gactggaaaa cgcgaatgct gcgtttcttc tgacaaacgc
    17461 tgataatcag aaatcgtatc tgtctgctgc aaataatgag tcttttcagc tggcatatag
    17521 atatcactga caagcatgat atcttttttg atcaattggt cgcttaacgc tcggttgatc
    17581 atggttgcga ctgtaaatgt atcataagag gtgcgtaaga tcggcatttc aagatcatca
    17641 gctagctttg caatctcagc agtcgtattg aatcctcctg tgatcaatac tgcggcgcca
    17701 tcttccaatg ctaatttttg gacttccgcc cggttgccga cgatcattag tgatcctggc
    17761 gtaatatagc gttccatggc atgtttagtc atcgcgccga tgacgaattt attcaagacc
    17821 ttgtctaatc ctgccgcccc gcctaaaacg tccccttcaa tgatcctgac gacctcgcca
    17881 aatgtcaaac gttcaatatg tttttttaat ttgcgttcga tacggatggt ccctactcgt
    17941 tgaattgttg aaactagtcc gacattttct gcatctttga ttgcacggta cgctgttcct
    18001 tcactgacat tcaattcttt tgcgatgctt cttacagaaa ttcgattgcc aacaggtaaa
    18061 ctttcaatat attctaaaat caaatcatgt tttgtcgcca ttttctgcct acacttccag
    18121 cgtttccgag acgctcatta tttttccttc actatcttcc agtaactcta caaaagattc
    18181 gggatcttgt tcaatgacag ggaaagtatt gtaatggatt ggaactactt tcttggcacg
    18241 aattgtttgc gcagcctttg ctgcatcttc tggccccatc gtatagttat cgccaatcgg
    18301 caagaaagcg acatcaatat cgaattcttc tgcaagcaat cctagatcac tgaaataagc
    18361 agtatcccct gcatgataaa tcgtttttcc ttctgcttga ataataattc cgctaggctc
    18421 gcccatatac tgaacgatac cgtctacttc atagcttgaa ctatgctgtg catgtacgaa
    18481 ttttaccacg ccgaatggaa acgcatgttt tccaccgata ttcataccga atgaatctac
    18541 tcctctgctg ttcgcataat cagcaatctc aacaatactg ataattgtgg cttgattttc
    18601 ttttgcaata ggaatcatat ctcctatgtg gtcactatga ccatgagtaa tcagcaacca
    18661 atctgtcttt acttcttctg cagttaagtc tgtcaatgga tttcctgtaa taaacgggtc
    18721 aatcatcacg cgttggccat tttccatttc aattgttaaa caggaatgtc catgatacgt
    18781 aattttcata ttttacacct cgcgttttct ctgtatcttt tttattaaaa acgatttcat
    18841 cacttgcaca tcaatgaaaa aatatagctg ctaaatatat tttcattgga ttgatagtac
    18901 agtatgtaca aagcaattgt aacagataat acaggatgca ccaagtacca agcagatata
    18961 atctcaagtt tgaggtctct gctctgctta gcttctcttc tcgtccctta actaattaaa
    19021 tcagcgtaaa taggatggac gatcttttct aaatcggcta cttttatccg aaggcttctc
    19081 ccgatttcac cgcttgacac aattacttct tctttttctt ttaaagaact gtctgctaaa
    19141 aaacaataat caggatgtgc gagttttatt ccaatagggg tgtttgctcc gtggggatat
    19201 cctgtatgca ttaatacaaa atccattcca ggaaaaccaa ctttacgatc tttcgtcact
    19261 tgtctcgttt ttttgtaatc taagtgatcg ttcaaaggaa tcaatgcgat gatcacccca
    19321 gttttgtttc ctttcactac gagagtttta catatcaaag ctggatcaat ccctcttttt
    19381 tgtagttctt ctgtgaaaga ttcttctacc acatgactaa aatcaagtgg ttgatagctg
    19441 atcttttgtt cttctaaata acgttcgact tttgtttgac tcatcattga ttcactccct
    19501 ttattctatc gtttattata aagcgaggga tgctgtattc aaaaattatt tgtttttcgc
    19561 aaaaaaagag caagacgagc actctataga gaggttcatc ttactctttt tgtttatttt
    19621 ttatttcgac gttgagtagc ttccataatg actggcaaga tcactgggcg gcgttttgtt
    19681 tgttcaaata agtaacggcc taattgatct cggatgtctt gctttaattt gctccactca
    19741 aaatcattgc tttctaaatg tttctcaacg attgtttcaa tcatattgct gctttctttc
    19801 atcagatcac ggctagcttt tacgtaaacg aatccccgtg aagtaatttg aggttttgaa
    19861 atgattttct tttctcgccg actgatagtg acaaccgcta cgaaaatccc gtcttctgac
    19921 aagatttttc gatcacgtaa aacaatgttt ccgatatcac ctatgcctaa tccatcaatc
    19981 attacatttt ctgcacttgt agttcctgca acactcatac gcttgttctt atattccagc
    20041 acgtctccgc gtccagtaat atagatattt ttgtatggca tccctactgc atgagccaaa
    20101 tctgcatgag cagctaattc acgatactct ccttgaactg gcacaaaata tgttggtttc
    20161 atcaaattga tcattaattg caagtcatta ggattggcat ggccagaaac gcgcatattc
    20221 tcagagatca acttcactgt tccaccagct cgataaatga tatcttccgt tttcgccacg
    20281 atggtttcca tcgcgatgct tggcgtagta gtgatataaa ccaaatctcc ttcttcaatc
    20341 ttgacattac ggtgtgtacc gtttgccatt ttttgcagag acttgatggg ttcacccatt
    20401 cggccagttt ctaaaatgat cagttcttct ggatcatagt ttttcatgtc tttcaacgta
    20461 acaagcagat cttcacttgg taattgaagt ttttctaaac gcatcgctgt gcgaataatt
    20521 cgttcgaaat cttgaccagt caagacaact tttcgttgtg ctttttctgc tgcatccaat
    20581 acttgttgca ctcgttgcaa attacttgct acacaagcga cgatgatacg cccttcccaa
    20641 tatttgatcg tatcaaatac ttcatctgct atttgggctt cagaagctac aggctgagga
    20701 ttttctgcat ttgccgattc acttagtaag gctaagactc cttctttgcc gatttccgcc
    20761 aatcgtgcat aatctgtttg atacattggg atagccgttt ggtcaaattt aaaatctccc
    20821 gtataaacga tactaccttc ttttgttttc agattgatcc ccactgaatc tggtatcgta
    20881 tgagtggtac ggaaaaagct cactactgtt tcaccaaagt ctatttctgt atgctcatct
    20941 accacatgga aatccttaaa tcctttggct gcttcattac gactgacatt caattttgct
    21001 aactcgatcg tcaactctgt cccgaatact ggtacagaaa tttttgacaa caagtaagga
    21061 agtgccccga tcgcatctgc atgtccatgt gttaaaaaga caccagctac acgatcaatg
    21121 ttttcttcta aatacgtaaa atcagggatt acgacgtcga tccccaacaa ctcattttca
    21181 ggatatttta gcccacaatc taaaataaaa atatcctcgc ccacttctgc gacatacatg
    21241 ttttttccat tttcacgaac gccgcctaat gggataattt taatggtact cacaaacttc
    21301 acctcttctt atgacatcca agtttaaaga tgtcgatttt cttctttatc taaatatatg
    21361 agccattcca atgaagcata tgcaaaatca ctatttctca acttatggag aatcagcagg
    21421 aaaattacca gcacatccgc gttctgacag ttggatgggc attttatttt ggatatactt
    21481 cattaacaaa atggatcttt tgcgaaacgc tatagccaga atacttatat aagtattcag
    21541 ctgttcccca ttattataca acaaataaac taaatcgcta gtatcagcaa ttgttagaac
    21601 aactatgcac gtaaaaatcc ctctgtagca tctaggattt cttttgctat tcctctttca
    21661 tttcttcttc gtcggtttga aacatacttt tttcatttta ccacattttt tgcaataact
    21721 atagtgactt tttatatata cagaacctca tgttgatggt gtgcaaacag cataagattt
    21781 aagctagctt taagataggt tgtctattat caaatgcatc tactaggagg gatgcaaaat
    21841 atgaatttta tgaaacgtgc atggaaaagt acaaaagtaa aatggggacg ttcattgtta
    21901 ttgtttgccg tgttcacagc cattcttatt tttgtgctag ctggcctcac gattcgaagc
    21961 gctgcagaag aagcagcgaa tcaagcacaa aaagaagttg gagcaacagt gactttaagt
    22021 gccaatcgag aagcttcatt taaaaaacag gaacaaactt cttcaagcgg ttctaccact
    22081 gaagaacgtc cagatccagg cagtttttct cttacaccag tttccttaac ggacgcggaa
    22141 aaaatagctg aattagataa tgtaaagagt tattcctttg aaagttccgc ctcagctatg
    22201 gcaaaagatg gaataactgc catcactagt tctgattcaa gtgaagacag ttcagaaact
    22261 actgatgcag gcggtgaacc tgctggagga atgggcggga atccgcaaat gactcaagca
    22321 gactttcaaa tcagtggtgt gtcagcaact gcacaaaata gtaatttttc tgatggaaca
    22381 gcaaaaatca cggatggtga aggaatagat gacagcgaca aaggcacaaa caatgtagtc
    22441 attgattcta ctctagcaga aacaaatgac ctggcagtcg gagatacttt cacaatcacg
    22501 agtactgaag atgaagatac cacttatgaa atgacgatca aaggaattta tgaatccagc
    22561 gaaacaagcg gcagtatggg tatgaaattc aacttcatga atccgtcgaa tacactttac
    22621 acttcttaca cttttgcaaa tgaactaaat gggacatctg acgataacac gatagattct
    22681 gctatttaca ctctatctga tccagataaa atggatgagt ttctcaaaga agctgagaag
    22741 ctgatcgaca cagatacatt tagcttacaa tcaaatgatt ccatgtatca gtcaatgttg
    22801 gaacctttaa acaacgtggc aagtttttct aaaaatgttg ttttactagt ggctgtagcc
    22861 ggtattatca tacttactct gatcattatg attaccattc gggagcgtcg gcatgagtta
    22921 ggcgtcttgc tatctttagg agaatccaga agtaaaatca ttctccaatt gtttaccgaa
    22981 gtggctattt gtatgattct tgcattagga gttgcttcgc ttagcggaaa cgtcgtcgcg
    23041 aatgccgtgg gacagcagct acttgaccaa cagacagaaa ccacgacaca aaaccagcaa
    23101 gctcctggcg gtggacaaat gccaggtaac agacaagaac aaggcggtca acgcgatggt
    23161 caacaaggaa ccacgaataa tccatttgct gtttctgatc aagtcagcga attggaaatc
    23221 aatgtcaaac ctgcacaatt gggactatta gccggagtag catttggaat cagtctgttt
    23281 tctgtctttt tagcttctat agggatatta agattaaacc caagaaaaat cctattaaac
    23341 taaaggagga atttgatgtt acggacagaa caattgggat tttggtatca agatccgcaa
    23401 gatgcgcttt ataaagatgt agaactgaat ttcgaagctg gaaaaatgta cgctatttta
    23461 ggagcaagtg gttctggaaa aacgactttt ctttcgctta tttctggact agatgcacca
    23521 aaagaaggaa aggtatacta tcaagatcgt tcactgaaca aaatcgggtt aagaagttat
    23581 cgtaaaaatg atgtctcgat tattttccaa gcttacaact tgcttcctta tttatcggct
    23641 ctagacaacg tcatagcagc aatggaaatc actaagacca agcaagagaa ttatcgacaa
    23701 gttgctttag atcgactggc tcgtgtagga attgatgaaa tattagcaaa aaaacaggtg
    23761 accaaacttt cgggaggtca gcagcagcga gtcgctatcg ttcgtgctat ctgctgtgaa
    23821 catcaactaa tcgtagcgga tgagcctact ggaaatctag atgaaacaac ttcacaagat
    23881 atcgtcaaac ttttccaaga aatcgcccat gaacaaaatc gctgtattat catcgtgact
    23941 catgagattg aagtagcaaa aagctgtgat gaagtctttg aactaaaaaa taaaaaattt
    24001 caaaaaatcg aagtttaaaa aacaggataa tgatcaaata aaaaatccga accatatctg
    24061 tctcgcagat atggttcgga ttttttattt gcttgattca attagtaata agtggttttt
    24121 attttttttc gtcttttcga gaaaacttgt ctttgacttc gtcgcctaaa tttcgagctt
    24181 tttctttgat gtcagcacct agtgattgtg ctttcccttc tgtttctttg ctcttgtcgt
    24241 ctgttgcttt gccgtatgct tcttttcctt caccgactac tttatctttc atatctttag
    24301 aacgtccttt taaatctgcc attgtgcttc ctcctcttag tttgtttatt gatctacctt
    24361 cagggtaaca tcaacaacag atacagacaa acagaacgct cgttttctaa aaaagatagt
    24421 tagagctgtt gattaaaaac ttttttcgaa agtcccccat actttgactt ctttttcttg
    24481 gaccattatt tctcctttag cgataactgt atcaatatca agcgtttctt tatccaagat
    24541 acacagatct gcatcagcac caatttcgat ttttcctttt ttatccaatt tcaatatacg
    24601 tgctggatta ctggttatag ctcgtaaggc gatttccaaa gggatacttt ctttttgcac
    24661 agcttctttg atccctacta gcaatgcctt ggcacttcct actcctaacc ctaggaaatg
    24721 attgttttca tcaaaataag gcaagcttcc ttgtccatct gaggtcatcg tgaaattatc
    24781 taaagaaaca tcttcctcga tcaatcgttt cagtgctttg ctgaagcgaa cttcaccatc
    24841 tgtcttctcc caaaaatcag gatcttcgct agcagtgaag tcaatcgtgc caccacgttt
    24901 ggcaaatgcc acgcaagctt caaataattc tggtgtgcga tttgcatgag taggcaaaaa
    24961 ttgtgtgatc ggaatttctg tttcttctac gattcgtgtc aatagctcta gctttctttt
    25021 tcctccgcct aagtggacat tgatgatccc agcttttccg gataacatgc ctcctacacg
    25081 agcatctgct actgcacgag cgaactcttc aaaacttggc tgagaagaac ggtgatcgga
    25141 tactgcgatt tctccgattc cgataatttt atcaattgtc aaaaagtctt tgatgataga
    25201 atctgttact gtttttaccg gcaatcggta cgaaccttcg tatacataag tggagattcc
    25261 ttctgcttcc agacctttcg ctttgctgat caacgcagtc atatctctac cttctccgtc
    25321 tgttcccaaa caaccgacaa ctgtcgttac accagctgta gtcaattggc tcaatgtgac
    25381 ttctggcgtt cgattttgat aaccgccttc tcctcctcca cctaaaatat ggaaatgaca
    25441 gtcaatgaaa cccggggtta gtacttttcc ctcacctgaa agttcttcta cttccactcc
    25501 ttcgtagccg ccttctagct gttcttctac agccaaaatc ttcccaccgg caatcagtac
    25561 atccattttt cctaagtaat ctggtgcaaa cacttcaatt tgtcgaatga ttttcataca
    25621 tttttcctct ttctttaagt ataactgatg ataggatctt aaaaaataat ccaaacgata
    25681 ggatctttac tcctttgatt ttaccgtaaa atgacggttc ttggagtcct tatctactgt
    25741 ttggattact tttttattta caggtctcca gtactactgc agacctattg caatggcaat
    25801 catgatgaaa atagttccta aaacaaacag gaatccttgc atcttgattt ggaatcttgc
    25861 ccattttgcc cattcgatcc gtgcaacacc tagcacacca atcaagctgg ctgatacggg
    25921 tgtaaaagcg tccataaaac ctgaaccaag ctgataagca agaactgcta cttgtctgga
    25981 tacacctact aaatctgcca gtggagccat gattggcata gtcaaagctg cttgtcctga
    26041 atttgatgta acaaccaagt tgaacaaact ttggaagatg tacatcgccc aagcaccaat
    26101 gacagctgga acaccggtca acgccgtccc gatcccatgc aaaatcgtat tcaaagctga
    26161 aggaacagat gcatcagatc ctcccaagac aagaagaatc cctttggcca tacctactac
    26221 gattgctgta ccagctaaat cagctgcacc agattggaaa gaagaagcga tgtcattgac
    26281 tttcattcca tctaatttga aaagaacagc aatgattcca gcaactaatc ccatcacaaa
    26341 gaattgagaa gcgatttctg gaatgtaata gcctttttga gtcacgcccc aaacgatcca
    26401 gacaaggaca actaacatct ctaataagat caatttatgc ccaagcatga attttttatt
    26461 ttcatcagat gtctttttga tatgagaacg gaaataatca tctgatttgt aagtcaacga
    26521 actttctggt tttttacgga ttttttctgc atatatcatc atataaccag cggctaatgc
    26581 agtaacaact acccacataa tcaatcggaa tgtcgcacca gacaacacgg ggattccagc
    26641 aatcccttga gccaccgcta cgctaaacgg gctcatccaa gaggtcgcat ttccgacttg
    26701 agatgcgaca taagtcacgg ttactgctac gatcgagtca tatcctaacg caatgacaaa
    26761 tggaaccatc accattgaga atggaattac ctcttctgcc atcccgaatg tagcgccacc
    26821 gaatgaaaaa gcaaagaata atagcggtaa agctaaacgc tccagtccct tagttttact
    26881 gataaacgca tagatacctg cgtcgatcgc tccggtacgc ataataatac caaaagcacc
    26941 tccaacaaca aggatcaatg cagcgatccc tacagcagat ccatacttat ctccagatac
    27001 tagtccttca aaaacaaaat ttaagaaacc aaagccgcca aaatcatctg ttccccatat
    27061 tttggctgtt ttatgcaatt ttttagaagt atcatagata ttttcgccgt actgatcgta
    27121 aagcacatca tctgtcaagg agatttcatc tagtttttcc tgcgtaagat ttttttctcc
    27181 gtcagcaatg actttttcca atcctttttc cggaacatcc agtttttcac gttctgctgg
    27241 atgatcttgc agttcttcta attgatcaaa cacatacgac ttatccagct cgtaagcata
    27301 acggaaggaa tcttgacgta aaacagtacg agtacttgtt tcgccatttg catctttgta
    27361 ttcaatatct tgcgtgctga actttcctgc tggaacgaca aaagtcagta tccatgcaaa
    27421 gatgactaca ccaaaaataa tgacatacgt atggggtgtc gataatttag tgaagtcgcg
    27481 tttcggtttt cctgaagaat ttgttttctt caaatttatt tcttcctttc ttttttcagt
    27541 aattttgatg aa
//
LOCUS       DAZAJZ010000030        27456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000030 DAZAJZ010000000
VERSION     DAZAJZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.030"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG86D_001252"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG86D_001252"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN3140407.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG86D_001253"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG86D_001253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN3140408.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACG86D_001254"
     CDS             8118..9086
                     /locus_tag="ACG86D_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140409.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACG86D_001255"
     CDS             9104..10009
                     /locus_tag="ACG86D_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140410.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACG86D_001256"
     CDS             10006..10812
                     /locus_tag="ACG86D_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140411.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACG86D_001257"
     CDS             10969..11937
                     /locus_tag="ACG86D_001257"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN3140412.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACG86D_001258"
     CDS             complement(12254..12778)
                     /locus_tag="ACG86D_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN3140413.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="ACG86D_001259"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="ACG86D_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN3140414.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="ACG86D_001260"
     CDS             complement(15031..16017)
                     /locus_tag="ACG86D_001260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN3140415.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="ACG86D_001261"
     CDS             complement(16040..16606)
                     /locus_tag="ACG86D_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN3140416.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="ACG86D_001262"
     CDS             complement(16620..17036)
                     /locus_tag="ACG86D_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN3140417.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="ACG86D_001263"
     CDS             complement(17493..19427)
                     /locus_tag="ACG86D_001263"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3140418.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19729..20664
                     /gene="pplA"
                     /locus_tag="ACG86D_001264"
     CDS             19729..20664
                     /gene="pplA"
                     /locus_tag="ACG86D_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFN3140419.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20754..21857
                     /locus_tag="ACG86D_001265"
     CDS             20754..21857
                     /locus_tag="ACG86D_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN3140420.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21870..22823
                     /gene="menA"
                     /locus_tag="ACG86D_001266"
     CDS             21870..22823
                     /gene="menA"
                     /locus_tag="ACG86D_001266"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN3140421.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23637..24983)
                     /gene="gor"
                     /locus_tag="ACG86D_001267"
     CDS             complement(23637..24983)
                     /gene="gor"
                     /locus_tag="ACG86D_001267"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN3140422.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25117..26370)
                     /locus_tag="ACG86D_001268"
     CDS             complement(25117..26370)
                     /locus_tag="ACG86D_001268"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN3140423.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26376..27299)
                     /locus_tag="ACG86D_001269"
     CDS             complement(26376..27299)
                     /locus_tag="ACG86D_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN3140424.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
BASE COUNT         8017 a         5820 c         5207 g         8412 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc
    19561 aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta
    19621 catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga
    19681 atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa
    19741 cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc
    19801 gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact
    19861 gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc
    19921 acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt
    19981 gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca
    20041 aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa
    20101 aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa
    20161 gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc
    20221 caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa
    20281 gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa
    20341 atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt
    20401 aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa
    20461 ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat
    20521 ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc
    20581 atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta
    20641 gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga
    20701 cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga
    20761 aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca
    20821 gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc
    20881 catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag
    20941 gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca
    21001 cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag
    21061 taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt
    21121 ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg
    21181 atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc
    21241 ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca
    21301 tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa
    21361 aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca
    21421 gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg
    21481 gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg
    21541 agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt
    21601 atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg
    21661 acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa
    21721 gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga
    21781 ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc
    21841 gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg
    21901 aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg
    21961 ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt
    22021 ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct cattctgaag
    22081 tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta ttgattcgaa
    22141 acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta acgatcaata
    22201 cgggttggct tttacttttg atgggattta tctgttgttt tattggagta ttctatacat
    22261 tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg gtgactatgg
    22321 gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat gtctatgatc
    22381 tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg gctatagcag
    22441 caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg ttggctaata
    22501 atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt ttttatatcg
    22561 gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat ttcgtgattt
    22621 taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc ttgaccttgc
    22681 caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct atcagtttca
    22741 gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg ctattactaa
    22801 caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg ccctgcatca
    22861 agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt tgggcttatt
    22921 accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag tcgcttagtt
    22981 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    23041 aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt
    23101 cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct
    23161 aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac
    23221 aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga
    23281 actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat
    23341 ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt
    23401 taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct
    23461 tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt
    23521 atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat
    23581 tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat
    23641 cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct
    23701 tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca
    23761 ataccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt
    23821 tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa
    23881 actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt
    23941 gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct
    24001 ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc
    24061 agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg
    24121 tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct
    24181 aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt
    24241 tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata
    24301 tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc
    24361 attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct
    24421 aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct
    24481 ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca
    24541 ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag
    24601 gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc
    24661 tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt
    24721 tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa
    24781 cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta
    24841 ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact
    24901 tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt
    24961 acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt
    25021 tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc
    25081 gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc
    25141 tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg
    25201 gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa
    25261 tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt
    25321 agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat
    25381 tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt
    25441 agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata
    25501 gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga
    25561 tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat
    25621 caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc
    25681 cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc
    25741 atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg
    25801 gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc
    25861 gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc
    25921 taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc
    25981 tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat
    26041 caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa
    26101 ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt
    26161 cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc
    26221 tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg
    26281 gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa
    26341 ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata
    26401 taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc
    26461 attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg
    26521 aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat
    26581 gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag atcattgctg
    26641 gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta
    26701 ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta
    26761 tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg
    26821 gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca
    26881 cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc
    26941 gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc
    27001 gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa
    27061 gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata
    27121 ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta
    27181 aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct
    27241 gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata
    27301 attttctcct cctacaactt cataatagta gcatagctct aaaaactttc caatacttct
    27361 tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat
    27421 attctgtcca aatataaaaa gagattgtga caaaaa
//
LOCUS       DAZAJZ010000031        27300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000031 DAZAJZ010000000
VERSION     DAZAJZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.031"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..482)
                     /locus_tag="ACG86D_001270"
     CDS             complement(138..482)
                     /locus_tag="ACG86D_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN3140425.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(619..1764)
                     /locus_tag="ACG86D_001271"
     CDS             complement(619..1764)
                     /locus_tag="ACG86D_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN3140426.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(1944..2918)
                     /locus_tag="ACG86D_001272"
     CDS             complement(1944..2918)
                     /locus_tag="ACG86D_001272"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN3140427.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2998..3873)
                     /locus_tag="ACG86D_001273"
     CDS             complement(2998..3873)
                     /locus_tag="ACG86D_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN3140428.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            4003..5910
                     /locus_tag="ACG86D_001274"
     CDS             4003..5910
                     /locus_tag="ACG86D_001274"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN3140429.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6451..9729)
                     /locus_tag="ACG86D_001275"
     CDS             complement(6451..9729)
                     /locus_tag="ACG86D_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN3140430.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(10000..10545)
                     /locus_tag="ACG86D_001276"
     CDS             complement(10000..10545)
                     /locus_tag="ACG86D_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN3140431.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10557..11249)
                     /locus_tag="ACG86D_001277"
     CDS             complement(10557..11249)
                     /locus_tag="ACG86D_001277"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN3140432.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11268..11552)
                     /gene="macP"
                     /locus_tag="ACG86D_001278"
     CDS             complement(11268..11552)
                     /gene="macP"
                     /locus_tag="ACG86D_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN3140433.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11565..12128)
                     /locus_tag="ACG86D_001279"
     CDS             complement(11565..12128)
                     /locus_tag="ACG86D_001279"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN3140434.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12356..13018
                     /locus_tag="ACG86D_001280"
     CDS             12356..13018
                     /locus_tag="ACG86D_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN3140435.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            13142..14338
                     /locus_tag="ACG86D_001281"
     CDS             13142..14338
                     /locus_tag="ACG86D_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN3140436.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14439..15815)
                     /locus_tag="ACG86D_001282"
     CDS             complement(14439..15815)
                     /locus_tag="ACG86D_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3140437.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15852..16719)
                     /locus_tag="ACG86D_001283"
                     /pseudo
     CDS             complement(15852..16719)
                     /locus_tag="ACG86D_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(16808..17833)
                     /locus_tag="ACG86D_001284"
     CDS             complement(16808..17833)
                     /locus_tag="ACG86D_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3140438.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17851..18525)
                     /locus_tag="ACG86D_001285"
     CDS             complement(17851..18525)
                     /locus_tag="ACG86D_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3140439.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18709..19602)
                     /locus_tag="ACG86D_001286"
     CDS             complement(18709..19602)
                     /locus_tag="ACG86D_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3140440.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(19676..21373)
                     /locus_tag="ACG86D_001287"
     CDS             complement(19676..21373)
                     /locus_tag="ACG86D_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3140441.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(21366..22679)
                     /locus_tag="ACG86D_001288"
     CDS             complement(21366..22679)
                     /locus_tag="ACG86D_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN3140442.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(22679..26620)
                     /locus_tag="ACG86D_001289"
     CDS             complement(22679..26620)
                     /locus_tag="ACG86D_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3140443.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(26729..27187)
                     /locus_tag="ACG86D_001290"
     CDS             complement(26729..27187)
                     /locus_tag="ACG86D_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3140444.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         7673 a         5972 c         4835 g         8820 t
ORIGIN      
        1 aaatcagcaa aatattaaaa ataacaacat gtgttaaatt taaaaataaa aaagacaact
       61 aatttgcgtc gtccttacaa agttagtcat ctaacaatag gccgtttcaa aactagttgt
      121 ctttttatat ccacagatca ttcgactttt tcgaataacc cacggctaat catcccatcc
      181 atcagaaaat aaaatttatc ttggctcatt ttctgttttg gatctttgaa aatattgact
      241 gctttcaatc catttgctgt agtgatcgac attttcaatg tttgaatatc ttttgataca
      301 gtgattttta atttgaatcc ttctttgctt tgcggcgtgg catctaatgg tcggatcaat
      361 tgataattgc ctccgtttgt ttctgtaaat ccattatcgc gcaatgtata tctttttgca
      421 tcaggatgca aacgatagct aattggtgcg cccagtaccg ttgctgttgt ttcaaatgcc
      481 atgattttca cctcgataaa attagtttct ctttattata aaagtttatc ctgattttta
      541 ctaggttttt cagttaagtt ccctaaaaaa tgaaggcgtt ctctcagaca tctgttcata
      601 taagaatgac ttcactagtt attttttgac agcacgaact aattgatcaa ggaaatactg
      661 gatgtcgtct tcggtatttc ccaaaccaaa gctgatacgg atggattctt ttagtatcgg
      721 tgtatcttct ccatacattg cttctaatac gtgtgaaggc tcgatatttc ctgctgtaca
      781 agctgaacca gtagaaatag cgaccccatt aagatcaagc ttcatcagta gcaaatcatt
      841 tgcttcgcca attaagcgaa gattcaacac atgagggagt ttattcgtta aatcaccatt
      901 tacttgataa gggatttcgt ttgcttctaa tgtatcaaga atcaattgct ggaatccttt
      961 atatttgttg ttacgtgctt cacgttcttc aggtgacagc agttcaactg cttttgccat
     1021 accccagatc cctgctaaat tttctgtacc tgcacgccgc ttttcttcct gctccccgcc
     1081 taaaagcaat gcagggatat ttactccgtc tcgtttatat aagaaaccga cgccttttgg
     1141 cccattgatt ttatgagcag agatacttaa taaatcgatt cctaattctt ctggatggat
     1201 cgtttggttt ccgtacgctt gtaccgcatc tgtatggaaa taagctggat gatccgctaa
     1261 aagttcaccg atttctttga ttggcatcaa atttccgatt tcattgttcc cgtacatcac
     1321 agagaccaag atcgtatctt ctcgtaacgc ttttttgacc tcttcgatcg acagattccc
     1381 tttttcatct acaggcagat atgtaacgtc aaaccccttt gtctctaaat atttcatggt
     1441 attaagaaca gcaggatgtt cgatagctgt agtgattaga tgtctgcctt ctttttgacg
     1501 cgagaacgct gtttctaaga tagctgtatt atccccttct gttcccccac tgttaaagat
     1561 gatctcgtga ggttttgctt gcagactgtc tgcaatgatt tgtcttgctg tttccaattt
     1621 ttcatgcgcg tggcgaccaa atccatgtat gcttgaagga tttccaaacg tgtcttgcat
     1681 gattgccgtc atcgcttcaa ttacttgtgg atgcatgggg gtagttgccg catgatccag
     1741 ataaatagtt ttcacttcat tcatcccttc caaattattg ctaggcacat tctagcataa
     1801 aatagcgaac aaaccaaaga agagctcatt tttgtctaga ggacgtttca tgggcataga
     1861 aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca
     1921 acttctttcc ctcttttttt tgtttattcg aattcttttt cttccagacg gaataacgga
     1981 ctcatcggtg tattttcatg gatgcgtttg accgcttcac ccattaattc tgaacatgta
     2041 acgattgtta agttttctgg atgacgattt tcttctgtga aaacagaatc agtgatacaa
     2101 atatctttga ttggtgcatt gtctaaaata cttttcgcat gatcggacaa taatccatgt
     2161 gacgcacagg catatacttc ttttgcgccg ttttctttta atacatttgc tgctcgtgca
     2221 agcgttgctc ctgtgttcaa gatatcatct accatgatac atgtctttcc ttgaacatca
     2281 ccgatcacat aaccaccctc agttgaagca tcatctgcat gatctacgat ggctaatgtg
     2341 ctgttcaaat attctgaaag actacgtgca cgctgaacac cgctgttttt tggcgaaaca
     2401 actacgatgt catctcccgt cagacctaat tgacggtaat aatgagcaaa caacggcatc
     2461 gtaaataaat tatctacagg aatatcaaag aatccttgta cttgaaccgt gtgcagatct
     2521 aaagtcagta ctcgagttgc ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg
     2581 atcggttcat gaggtttcgc cgtgcggtct tgcctagcat acccatagta aggcaatacg
     2641 acattgatcg ttttcgcact tgctcgcttc attgcatcga tcatgatcag tagttccata
     2701 tagtaatcat tgacaggtgc attcgttgat tgaacgatat acacatgatc ccctcgaata
     2761 ctttcttcga tattgatgga aatctcgcca tcactaaatt gcttcacgac gcttttacct
     2821 aattctgttc cgaacacttt cgcgattttt tcagctaatg gacggttgcc attcaaacta
     2881 aaaatcttca acgtgtcatc ctgatactta ttactcatgt agtcctcccc aaaacttctc
     2941 tattcttcga tagtttaaca tatttttcca agattttctg ttaatttcag caaattctta
     3001 aggaagtgaa actgttccgt tactccaact ggcaggaatg actttatgat aatcgcccca
     3061 gttgtcgtgt actttgtaat aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt
     3121 cacccaatga ttgccatatg tactgccaag tactttcaaa ataccaatca tcacaggttt
     3181 gccttcacgt atacgttttg tagctcgctg ccatgaacct agaagagttg ctcgtgcacg
     3241 ctcatgattg ccattcttct ttaaaaaact agaaatccct gtactaacct gaaaaggaat
     3301 cgtaggaagt cctagtggtt ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc
     3361 agccgtattt ttcttgcgaa tctcatttgg aagcatccac cccttacgat agtcttgata
     3421 ataggcaagg agaactgctg ccgcgtaagt accacaaaga tagcctgagg gggtagccca
     3481 tttcttatac ctcaaaaaac gttcttctgg caatcccacc cagcaatttg cgagttcctt
     3541 tcctgacctt ttttcttcga tcaaaacggg aatatcttct tttctgttga tccatctaga
     3601 taactcgatt ggctcttcat tgatcagctt ccatttttta caagaaagac cctcttgata
     3661 agtatatttt gctggagaat attctaccgg atgtcctgat tgatcgataa tcacataact
     3721 ttgatcttca aaaaccagag ctaaaaaaag caatttactt tttcttgact gcagctgctg
     3781 agtgctggct aattttttaa tttttttatc tactttgaaa tataaaggtt tatccactac
     3841 ttgattcagt tgggatcgcc tccttttttt cattataagt gaaggattga aaaaaacaaa
     3901 tcagtttgat tgttcgaatt ttcaattttt tatgttctat ttgttaattc cttcattttg
     3961 taagataatg ggcacacgaa acaaaaaagg agcgatcgat gaatgaacaa agaacgattg
     4021 aaacaagatt tctatgaagc agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca
     4081 gataaaccag ctacaggcgg tttccaagat ttagttgacg ggatcgacca acttttgatg
     4141 ggagaaacag acgatctgct tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat
     4201 ttccttgcat attatcaact ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa
     4261 ccgcttcaac ctataatcga aaaagttgaa tcattgcagt ctttcgacga attgaataag
     4321 caatttccag aatggctgtt ggatacatta ccattgccat ttaatctgga tgtagatgca
     4381 gatatgaaga atgcgcaaac gaacgccttc tttgcttatc cgccttcttt attccttcca
     4441 gataagacgt attatgcatc agaccatccc aacggtgcac aactaatagg cgttttcttt
     4501 gatatggctg ttcagttatt agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc
     4561 acggaacaag cagtgcaatt tgataaattg attgctcctc atgtaaaaag tgcagaagaa
     4621 aacgcagatt acagcaaaat gtataatccg caatcttttg atacatttat taaatacaca
     4681 gataaactgg acttgaaaaa acttgtcacg ggtttgattg ggacaactcc agataaagtg
     4741 atcgtaactg atccagtgta cttcgatcat ttgaatgaca tcctgacaga agctcatttt
     4801 ccgttattca aaagctggat gatcattaaa acagtacgtt ctcttagtgg ttatctatca
     4861 gaagaattcc gccaagtcag cggaatctat tctcgcgtat tgtctggaac agacgaagca
     4921 atgcctcaga aaaaagcagc gtattacctt gcctctggac aattcgatca agtagttggc
     4981 gattactacg gaaaaaaata ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg
     5041 aaaaaaatga tcgccgttta taaaaaacgt ctagaagaaa atgactggct tggggatgca
     5101 acaaaagaaa aagccgtcat aaaattaaat aacttaggta tccaagtagg ctaccccgat
     5161 aagatcccag cattattcga ccagttcaaa acagtacctg cagatcaggg tggtactttg
     5221 ctttcaaata ctcttgtttt cagcaaactc acattaaaag atcgtttcag caaatggaac
     5281 aaaccagtgg atcgttcaga atgggaaatg agtgctgaca cggtcaatgc ttattatcat
     5341 ccatttcgga atgtgatcgt ctttccggca gcgatccttc aagcaccatt ttacagtcta
     5401 gaacaatcaa gctctgctaa ttatggtgga atcggagctg ttatcgctca tgagatttcc
     5461 catgcatttg ataataacgg cgctttattt gacgaatatg gcaacttgaa caactggtgg
     5521 acagaagaag atcttacaca atttcaaaac aaagcacaag caatgatcga agaattcgat
     5581 ggtctgcctt ttgctgacgg aaaagtaaac ggaaaattaa ccgtttcgga aaatattgct
     5641 gatgcaggcg gtctaagctg tgcattggaa gcagcaaaaa cggaatcaga tctttctatc
     5701 gaggaattct ttgtcaattg ggcaacgatt tggcgtacaa aagcaaaaaa agaataccaa
     5761 caacttttac tgcaaatcga tgtccatgcc cctgctaaac tccgagcaaa cgtgcaacca
     5821 aaaaaccttg acgaattcta tgaaacattt ggtatcacgg aacaagacgc tatgtatcta
     5881 gcgccagaaa aaagagtgcg aatctggtaa gaggttgtgc cagaagtggt cagcttcaag
     5941 aaataagaag gaatttggga aaatcgcttc tcaaattttt gtgacatttc ggcttatttt
     6001 tcgaagaagt tacttctgga ataccatgta ttagaggttg tgaaaaagct gcttagtttc
     6061 aagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttac
     6121 taccgagaaa ctggcttttg aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac
     6181 ctgagtatta gagaaaaaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
     6241 ataccgaggg tcagctgatt gaacaccgtt tagtggagag attgtgaaaa agctgtcctg
     6301 catcaagtaa taagaacagc caatcaaaag cagcaaggag aacctcatct ataaaaagat
     6361 agaaggaagt gagacatcta ttggtctcat ttccttctat ttttactttc ctcagtcaat
     6421 tgttttacat cataaaagga gaaattccac ttattttttt cgctgtttca tgacataaac
     6481 aactacaccg attaacaaaa ttcctccagc taatgctagc aacgggttat taaattctcc
     6541 cgtttttggg agtccttgag tgccattttt ttcactttct tttgctgtcc tatccggatg
     6601 agaaggtttt ccatttggtt ttccatttgg tgattcattt cctggatttt ctctttctgg
     6661 tttgtcattt cccgggggca catcggcagt ttctcgcaca gtgatcgctt tttgctgggt
     6721 aacagattct gtatgagcag accatttttc cccatcaaat atttccgaac gataagtgac
     6781 ttctagcgta tattctcctt ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg
     6841 agcacttgac tgtgtttcaa cgatttggat cttttctggg aaataacgca tatcttcaaa
     6901 gacaggcgtt ccttgatttg gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc
     6961 aatgatattt ccttctgcat tctccgccac accatttgct tcttgtataa taatcggatt
     7021 attttgttca tttggttttg tctgatttgg ttcttgtacg attgcatact ctggatattc
     7081 cgaagacggt gaggtttctc cgtcaaaaat agcacctaca gataatggaa gaccatccac
     7141 tctttccttt tctgtttgac gattccccgg tttaatctgc atcagtccat gaacatggat
     7201 acttccattt tcatagcgcg taacaggctg ctgaccttct ttcgaacatg tatttagaga
     7261 ctcgatccaa tcttttgata tggtatgtcc aagagtattt tctgattgtt tctctcgttc
     7321 attccaatag tagttcaacg agtggtagat ttgatcttcg ttttgattca atgggcggat
     7381 ctgctctctc atctcaagat tctctgttcg gaaagataag ataccagaag catggtagtc
     7441 tgtgtttcct gctgaagagg tataaagagc cacattatat gaaccgttat taaaacttgt
     7501 actgctattg acgataatat ctgttccgct attggaatcg attcctttcg caagattttc
     7561 gaaagacaaa ctattgatca attcatgagg gcccgttaaa gaagaaccgc ccattttgaa
     7621 tccattaccg ttaccttctc cctcaattcc tggtacccag ccgtttcgat aagcaactga
     7681 gtttttgatg attacttttc cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc
     7741 gtcatctgca ttatgatacg cgatacaacc gtcaaataca tttccttctc ctattgtcag
     7801 tttggccgca aatccatcgg catcttcaaa tcctggatcg gcattttcaa aagatgtaca
     7861 gttcttgact aagttatgcg cgggccatgc ttcgaaagga tcatttgagc taccgctgat
     7921 ttggattcct gtatttccat tccgataagc atgaatctct tcgatcaaat tatgatttcc
     7981 agaaatctgt aatccttttt gcatagctgc actatttgta atgtcaaacc ctcgtaaacc
     8041 ccagtagtga ctgaacagtg tcatcccatt tccagttcct ttaaaatcaa gtacaggacg
     8101 cttgttttct ggatttttct ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac
     8161 gccttttgga atcgtcaatc ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt
     8221 ctgcccagca tatgcgtatc tcaatgcttg tgtcagagat aatggatttt gtctgcttcc
     8281 gtttcctgca tctgaccctt ctggtgttac ataaactgtc gaatatttag gtttacggta
     8341 gtccactgta tgaagaatct cttttgtttc ataactagtc atctctacgt attcttctgg
     8401 cggatagttt ttatctggcg taaaactgaa agtaaattcg ttcgttcctt ccatcagttt
     8461 tgtctgataa tcaaattccc gattcgcagt gatagccaag ttttttgcgt tcttaagcac
     8521 ctctcctgtt gctttatttt taattgtcaa taatccgtca gcatttgctc taaaggacat
     8581 agtatgatct tggtttcctg aatgattact agaggtcaca gtgtaaatcg gatcaatcaa
     8641 gcgagtaggt ctttcttgcc caggaggatc tgtcgcagga tctgagtaag caacttcgat
     8701 tccttctacc ttgatcgtca tattccttgc tgcagcaaat cctgcataaa taaccgattc
     8761 atctgaagcg tataattttt cccagtcata catgatgtca gaagccattt cttctcctgt
     8821 ttcaggatcg taataatatg cctctaaacc agtattcgtt tttttcatct ctacgtccag
     8881 tttagtaatc gagttcgtgg ccggaaacag attcccatga ccgtttccga atatattata
     8941 agaacctgtt tctttttcca agaaccctgc tgaagtctct aaaggtgttg tttccactga
     9001 gacactccca ggagctggcg cagcttgcgg atcttcagat gatattcctg tgattgcccg
     9061 tgtccccaat ccaagtcgca tcgtatattt gctgccgctc gtgttgacca cttcttgtct
     9121 atccgagtcc cagacatatt cgatccgact gcctagtatg gcaaatgaat ttgaataaaa
     9181 tgcctgccca aacaaatgat tttttggtac tgcgtctctt accattaaag caaatccttc
     9241 ttgggcattc gtataggtcc aagattctat ttcgatcgtc gcccgcatcc taaagttgat
     9301 gtctttgggg attgcttgat aataataggt cattccatct gatccgcttg actggaattt
     9361 tccgccgtta ttcgtggaag agagccgcac gccgtcttct aatggttcaa tggcatacct
     9421 actagcactt gtgctttctc cgaaaacaac ccctttccac tcttcatttg cttcttcaga
     9481 ttgctttttt gtcttttcat tttctgattc tttcatcata ggatcatttc cttcttccgt
     9541 atccatatct tcactagttc ttggtatttc ctccattgtt gacatccctt caatgttcac
     9601 gtgtgcattt tcttcgttca cagactgttg ttcgttagca gcaacttcaa cgacagaaat
     9661 taaaaccggg gtactgagca taaggagatt catcattagc gcactgtatt ttatacgttt
     9721 ttttaacaag tgtattcctc ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta
     9781 atttattttt actgtcaata aaaaacaaaa ttataataag tgtattttat aaaaagaaaa
     9841 aatataactt aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt
     9901 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
     9961 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
    10021 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacgga
    10081 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
    10141 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
    10201 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
    10261 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
    10321 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
    10381 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
    10441 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
    10501 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
    10561 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
    10621 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
    10681 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
    10741 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
    10801 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
    10861 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
    10921 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
    10981 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
    11041 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
    11101 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
    11161 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
    11221 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
    11281 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
    11341 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
    11401 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
    11461 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
    11521 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
    11581 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
    11641 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
    11701 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
    11761 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
    11821 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
    11881 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
    11941 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
    12001 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
    12061 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
    12121 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
    12181 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
    12241 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
    12301 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
    12361 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
    12421 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
    12481 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
    12541 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
    12601 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
    12661 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
    12721 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
    12781 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
    12841 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
    12901 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
    12961 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
    13021 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
    13081 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
    13141 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
    13201 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
    13261 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
    13321 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
    13381 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
    13441 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
    13501 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
    13561 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
    13621 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
    13681 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
    13741 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
    13801 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
    13861 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
    13921 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
    13981 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
    14041 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
    14101 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
    14161 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
    14221 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
    14281 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
    14341 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
    14401 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
    14461 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
    14521 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaaaat ggagacgatt
    14581 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
    14641 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
    14701 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
    14761 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
    14821 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
    14881 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
    14941 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
    15001 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
    15061 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
    15121 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
    15181 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
    15241 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
    15301 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
    15361 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
    15421 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
    15481 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
    15541 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
    15601 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
    15661 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
    15721 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
    15781 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
    15841 gaaaacagcg attaaactgt tttcatatac cctaaaaggg ataccaccta aaatgactaa
    15901 aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa
    15961 aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa
    16021 accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca
    16081 gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc
    16141 gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca
    16201 gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc
    16261 cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc
    16321 agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt
    16381 cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa
    16441 accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc
    16501 attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt
    16561 gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat
    16621 tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac caatactacc
    16681 ccatgccaac atagggacta atgctattaa aatttccata accaatctcc ttttatttca
    16741 catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa
    16801 cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg
    16861 tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt
    16921 ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag
    16981 taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt
    17041 taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat
    17101 ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag
    17161 catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa
    17221 aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga
    17281 tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt
    17341 catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact
    17401 cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg
    17461 agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt
    17521 gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac
    17581 cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc
    17641 ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag
    17701 ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga
    17761 tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt
    17821 taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt
    17881 tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt
    17941 gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt
    18001 ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt
    18061 tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt
    18121 aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag
    18181 taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc
    18241 tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat
    18301 gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg
    18361 aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg
    18421 attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa
    18481 ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat
    18541 ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga
    18601 aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc
    18661 tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt
    18721 tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag
    18781 cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg
    18841 ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg
    18901 gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct
    18961 aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc
    19021 gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc
    19081 cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc
    19141 tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc
    19201 attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat
    19261 catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa
    19321 aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt
    19381 cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt
    19441 ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg
    19501 atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag
    19561 actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt
    19621 taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata
    19681 cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc
    19741 cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca
    19801 tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat
    19861 agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac
    19921 cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa
    19981 agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc
    20041 tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt
    20101 cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg
    20161 catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt
    20221 cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat
    20281 catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac
    20341 gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa
    20401 tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa
    20461 tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg
    20521 tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga
    20581 aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat
    20641 aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt
    20701 cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat
    20761 ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca
    20821 tcatcactgg catcataaca accattggag acgcgattct ggaaatatga gctggcgaat
    20881 aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga
    20941 cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc
    21001 gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag
    21061 aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata
    21121 cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg
    21181 tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta
    21241 atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt
    21301 tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca
    21361 tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat
    21421 aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac
    21481 cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt
    21541 tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact
    21601 ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct
    21661 tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc
    21721 tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt
    21781 tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc
    21841 aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata
    21901 tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat
    21961 ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt
    22021 agataaccga catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc
    22081 gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc
    22141 aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg
    22201 tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc
    22261 ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata
    22321 ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca
    22381 gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt
    22441 ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca
    22501 tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc
    22561 aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg
    22621 actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt
    22681 accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg
    22741 ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca
    22801 acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa
    22861 gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg
    22921 aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt
    22981 caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt
    23041 caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata
    23101 gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac
    23161 tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa
    23221 ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac
    23281 catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt
    23341 caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat
    23401 acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt
    23461 ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga
    23521 tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca
    23581 gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc
    23641 cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg taagcagtga
    23701 atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt
    23761 cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg
    23821 tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt
    23881 ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg
    23941 gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct actgaataga
    24001 acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg
    24061 gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga
    24121 ttggcatatt actgccttca attagtttat tgatattcat gtggattggc ttgaattcat
    24181 ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt
    24241 gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg
    24301 gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct
    24361 catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat
    24421 aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat
    24481 ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg
    24541 tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc
    24601 cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt
    24661 cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat
    24721 cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc
    24781 ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg
    24841 gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt
    24901 ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc
    24961 cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg
    25021 gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg
    25081 tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc
    25141 gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag
    25201 catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac
    25261 ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg
    25321 cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac
    25381 caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat
    25441 ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg
    25501 cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag
    25561 caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac
    25621 ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt
    25681 tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa
    25741 gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc
    25801 ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt
    25861 tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg
    25921 ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag
    25981 tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa
    26041 cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt
    26101 aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt
    26161 ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt
    26221 catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg
    26281 atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac
    26341 tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat
    26401 tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc
    26461 gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag
    26521 ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg
    26581 cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt
    26641 gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc
    26701 tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg
    26761 tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt
    26821 cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac
    26881 ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac
    26941 gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt
    27001 cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat
    27061 cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact
    27121 ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc
    27181 ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa
    27241 caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt
//
LOCUS       DAZAJZ010000032        27203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000032 DAZAJZ010000000
VERSION     DAZAJZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.032"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG86D_001291"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG86D_001291"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN3140445.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG86D_001292"
     CDS             complement(937..1518)
                     /locus_tag="ACG86D_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3140446.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG86D_001293"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG86D_001293"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN3140447.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG86D_001294"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG86D_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN3140448.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG86D_001295"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG86D_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN3140449.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG86D_001296"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG86D_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN3140450.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG86D_001297"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG86D_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN3140451.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG86D_001298"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG86D_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN3140452.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG86D_001299"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG86D_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN3140453.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG86D_001300"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG86D_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN3140454.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG86D_001301"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG86D_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN3140455.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG86D_001302"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG86D_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN3140456.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG86D_001303"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG86D_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN3140457.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG86D_001304"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG86D_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN3140458.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG86D_001305"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG86D_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN3140459.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG86D_001306"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG86D_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN3140460.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG86D_001307"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG86D_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN3140461.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG86D_001308"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG86D_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN3140462.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG86D_001309"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG86D_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN3140463.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG86D_001310"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG86D_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN3140464.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG86D_001311"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG86D_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN3140465.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG86D_001312"
     CDS             13972..14157
                     /locus_tag="ACG86D_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN3140466.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG86D_001313"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG86D_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN3140467.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG86D_001314"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG86D_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN3140468.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG86D_001315"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG86D_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN3140469.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG86D_001316"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG86D_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN3140470.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG86D_001317"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG86D_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN3140471.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG86D_001318"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG86D_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN3140472.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG86D_001319"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG86D_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN3140473.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG86D_001320"
     CDS             18232..18879
                     /locus_tag="ACG86D_001320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN3140474.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG86D_001321"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG86D_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN3140475.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG86D_001322"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG86D_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN3140476.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG86D_001323"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG86D_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN3140477.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG86D_001324"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG86D_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN3140478.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG86D_001325"
     CDS             20317..21255
                     /locus_tag="ACG86D_001325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN3140479.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG86D_001326"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG86D_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN3140480.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG86D_001327"
     CDS             21935..22774
                     /locus_tag="ACG86D_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3140481.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG86D_001328"
     CDS             22750..23619
                     /locus_tag="ACG86D_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3140482.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG86D_001329"
     CDS             23609..24406
                     /locus_tag="ACG86D_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN3140483.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACG86D_001330"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACG86D_001330"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN3140484.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26019..26213
                     /locus_tag="ACG86D_001331"
                     /pseudo
     CDS             26019..26213
                     /locus_tag="ACG86D_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26401..>27203)
                     /locus_tag="ACG86D_001332"
     CDS             complement(26401..>27203)
                     /locus_tag="ACG86D_001332"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3140485.1"
                     /translation="INKKETTFMNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFI
                     SKGPFPSPRKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLF
                     VQDENDANEIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKH
                     STEKTLYFTGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKA
                     IPDSTIIAVHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT         8811 a         5005 c         5770 g         7617 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggt attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact aagtacttta
    26341 ttttactctt taggattgat acagtagcat ctatcccttt attaataatc ttttattttt
    26401 ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa ttcctttttc
    26461 tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag cttccatatg
    26521 aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat cgtctttccc
    26581 cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta ctatgatttc
    26641 tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat ctcctgtaaa
    26701 gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac ctacataatt
    26761 caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag attcaaaaac
    26821 tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt cattcgcatc
    26881 attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg ctatatcgtc
    26941 ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga taatatcttc
    27001 gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg atggaaaagg
    27061 acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat caattaaaaa
    27121 ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta tattcataaa
    27181 cgttgtctcc tttttgttga tat
//
LOCUS       DAZAJZ010000033        26726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000033 DAZAJZ010000000
VERSION     DAZAJZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.033"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACG86D_001333"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACG86D_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACG86D_001334"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACG86D_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACG86D_001335"
     CDS             complement(1147..1716)
                     /locus_tag="ACG86D_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3140486.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACG86D_001336"
     CDS             complement(1790..3079)
                     /locus_tag="ACG86D_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140487.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACG86D_001337"
     CDS             3406..4281
                     /locus_tag="ACG86D_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3140488.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACG86D_001338"
     CDS             4295..4618
                     /locus_tag="ACG86D_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140489.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACG86D_001339"
     CDS             4615..5367
                     /locus_tag="ACG86D_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN3140490.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACG86D_001340"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACG86D_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACG86D_001341"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACG86D_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN3140491.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="ACG86D_001342"
     CDS             complement(9831..12017)
                     /locus_tag="ACG86D_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3140492.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACG86D_001343"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACG86D_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN3140493.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="ACG86D_001344"
     CDS             complement(12239..12679)
                     /locus_tag="ACG86D_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN3140494.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13251)
                     /locus_tag="ACG86D_001345"
                     /pseudo
     CDS             complement(12753..13251)
                     /locus_tag="ACG86D_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13536..15002)
                     /locus_tag="ACG86D_001346"
     CDS             complement(13536..15002)
                     /locus_tag="ACG86D_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3140495.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15313..17394)
                     /locus_tag="ACG86D_001347"
     CDS             complement(15313..17394)
                     /locus_tag="ACG86D_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3140496.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17536..17658)
                     /locus_tag="ACG86D_001348"
     CDS             complement(17536..17658)
                     /locus_tag="ACG86D_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140497.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            complement(17918..18277)
                     /locus_tag="ACG86D_001349"
     CDS             complement(17918..18277)
                     /locus_tag="ACG86D_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN3140498.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18307..18648)
                     /locus_tag="ACG86D_001350"
     CDS             complement(18307..18648)
                     /locus_tag="ACG86D_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140499.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(18645..19319)
                     /locus_tag="ACG86D_001351"
     CDS             complement(18645..19319)
                     /locus_tag="ACG86D_001351"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3140500.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="ACG86D_001352"
     CDS             complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="ACG86D_001352"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN3140501.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21591..22781)
                     /locus_tag="ACG86D_001353"
     CDS             complement(21591..22781)
                     /locus_tag="ACG86D_001353"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3140502.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(22802..23329)
                     /locus_tag="ACG86D_001354"
     CDS             complement(22802..23329)
                     /locus_tag="ACG86D_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN3140503.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23376..26165)
                     /locus_tag="ACG86D_001355"
     CDS             complement(23376..26165)
                     /locus_tag="ACG86D_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN3140504.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26312..26512)
                     /locus_tag="ACG86D_001356"
     CDS             complement(26312..26512)
                     /locus_tag="ACG86D_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3140505.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT         7491 a         5669 c         4699 g         8867 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtagctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttcctttttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12901 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    13081 gtagaagtat aatttccttc aacgatccat gacggattag atactataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    13201 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    13261 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    13321 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    13381 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    13441 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    13501 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13561 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13621 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13681 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13741 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13801 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13861 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13921 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13981 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14041 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14101 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14161 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    14221 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14281 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14341 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14401 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14461 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14521 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14581 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    14641 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14701 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14761 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14821 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14881 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14941 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15001 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15061 acaaacttac aagacagtgt tattttatgt gtgtattatt ttatttaata cacatcgttt
    15121 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg accttattga
    15181 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
    15241 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
    15301 cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat tcaaaacagc
    15361 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
    15421 acctaaggaa agtaccaaaa tttttatccc aatagcaaag acaatgtttt gtttgacgat
    15481 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
    15541 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
    15601 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
    15661 tttattgtta ctttgtaaga tttcctctaa ccgctgcact ttatcttctg gaagcaattc
    15721 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgagtc
    15781 gcccgtcaac atgactgtgt ttttcacacc agcctgtttt aattgaacca atgcgtctac
    15841 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgattgc
    15901 tagataaagc aatgtaccga tttcttgact tgaaggaaaa gagatttcaa atctttccat
    15961 caatttgtga ttacctacta atacttgttt gccatcaact tcagctaaga tcccttcacc
    16021 tgcaaattcc tggatattag ctgttggtaa aagcggacct gcgtagcctt ctaaaacaga
    16081 ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca atatattgca tgaatatctt
    16141 tggatcgatt tttgtatcga tcgtctggat cgaaaagtcg cctttcgtta atgtgccggt
    16201 tttatcgaat accactgtct cggtttgcgc aagcaactcc aagtaatttg aaccttttac
    16261 taatacaccg attttacttg ctcctccgat accgccaaag aaactaagtg gcacggaaat
    16321 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
    16381 aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcctac
    16441 aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg gtgctttttt
    16501 actgctggca ttttctacta attctaataa tttgctgata gtagaatttt cataagattt
    16561 ttctacttga actaaaaatg gtttattttt attgataaat ccgcttaata ctgtttcacc
    16621 aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga ctaatgattc
    16681 tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa gtacaaactg
    16741 accgatcagc acgtcttcag ggttcacggt catcaatcct tccgccgtct gcacgtgagc
    16801 tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac tgactgccaa
    16861 tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt ctggatattc
    16921 accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat caaagatttt
    16981 tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca gcagataaat
    17041 caataaatac cctatcaagc gaattagttc attaaaatca aataatagca ataaagcgat
    17101 acctgcaagg gaaaataaaa tccggaacag cgtatttctc tctttttcat ttccatgttc
    17161 agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt ccttcactac
    17221 tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc tccgcgtcaa tcgttagttt
    17281 tccagaagtg aagtttaccg ttgcttcctt gactccattg attttctgaa ctcctttttc
    17341 gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt tcattttaat
    17401 gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg aatggttatt
    17461 catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct gcatcggaaa atttcttcca
    17521 tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgccactg ctcttatcca
    17581 acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc ctgttgctgg
    17641 caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa tccctgcaaa
    17701 atgtccttcc cagatatacg aagctccact acgctttcaa aaatatagaa agagttcttt
    17761 aatgaaaatt atttcaactt tccataaaca gtaaccccat ataaataaaa gacgtaaaag
    17821 tcacatggaa gaacttatac aggaaaacgc atagagtcag gtgaaataca tgtatcccac
    17881 ctgactctat gctttatcta acctttatta ttctctttta tacaatttct gcaccgaatg
    17941 gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc cctatgatcg
    18001 tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg cctgaaacga
    18061 tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt acttctttgc
    18121 caaaaggcgc aaaagaaagg aaggcgatct taaaacattg cataccgatt gggataccta
    18181 tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg attagtccgc
    18241 cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt ctattactgt
    18301 ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt ccagcatcaa
    18361 atattctgca aatttttttt ggatttcttc cgtattttct tttgttacta atgtgtgttg
    18421 gattagtttt cctgatggtc gctgacccac ccgttcttct gactgttgca cgtccgcttg
    18481 cgcaaaaccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt tattcttatc
    18541 atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag taatcagctg
    18601 attgatttga ggtagatctt cttttactgc ccaccgtatt tccatcattt ctcacttttc
    18661 tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc tatccagctt
    18721 tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct tgtatatttc
    18781 gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac cagcaatgcg
    18841 ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc tttacgccag
    18901 ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat gttgcagatt
    18961 cccgcttttt gaagttcatt tagtaatttt cgataaacgg attcaaattg ctgagcagat
    19021 ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt gattccgatc
    19081 aaaatcgata catagtcagg ttgtaaagct aaacaatctt ctcgccagcg tacttgcaga
    19141 tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat cgttggatat
    19201 tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc agttgcttct
    19261 gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg ttttttcatt
    19321 tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat cccataagcc
    19381 ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag tcccttcaaa
    19441 caattgaatg aacgctcatc aagtgtctag ttttttatgc tatttttttc ttaacgtgct
    19501 ggattcccct tgattccttc attgtttggt ccagagagat cgatcacacc taagcttccg
    19561 tcaatcagac cacttgatga cgccatcaga acatatattt ataggctcat ctaagacttc
    19621 cagtaaatgt tcctttacta atatctcttc tagattgctt gggatagatt ggccataact
    19681 ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggatcat tgcatgctga
    19741 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tgctagatgc
    19801 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt
    19861 gtatatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
    19921 tttgctttct tctcttatct gctggaaact tcctttatcc cgtatttaaa aaaaaagaac
    19981 tgcgagagtt cgcagttctt ttttttaaat aaacgttgct ctccggccaa ctccttggtc
    20041 acaagtcaca atagtcatca aacatgaaaa aatgtttttt tgaaattact ccttgtgact
    20101 cggagctgaa cggcagacgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    20161 cgatattagc tcaaaaaaca gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    20221 tactcaggtc aaggcactga tttcacaacc tcttatggat aaattcggtt caacaaacgt
    20281 gggaatggaa tagcttcacg cacatggtcg ataccagcaa tccaagtgac tgttcgttca
    20341 aggcctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc aagataccat
    20401 gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa atcatagtct
    20461 gttgcccgtt cgcttccacc aataatttca ccgtaacctt ctggtgcgat catatctgca
    20521 cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt gatggctttt
    20581 ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt ttcatgaggt
    20641 gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag taattcgatc
    20701 gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa gacttctttg
    20761 tctcgtccta gtacatctaa tgcgtattcg caatgatcta aaacattttg tactaagaat
    20821 gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc catttctggt
    20881 tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc acggaaagtc
    20941 ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta taattggcca
    21001 ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc cgtagtgcct
    21061 tctggtgcag aaccagtcaa gatcggcgga tcgattttga caaaatgatg ttgattaaag
    21121 aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg ttgtcttgaa
    21181 gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc ttttggtgtg
    21241 atcggataat cgtggctctc gcttaccact tccacatttg taattccgat ttcataacca
    21301 aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt ttcttggtgt
    21361 agatttttag ctgtttggaa aacttcttcc ggcacttcgc tttttacaac tacaccttgg
    21421 aaaaaggctg ttccgtctct taattgcaaa aatgcgattt ttccgcttga tcgcttattt
    21481 gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc gatgatttga
    21541 atttgttcca cgtatgactc tccttttata ttccttgaat ttcttcagga ttaattttga
    21601 cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt agcgcgatca
    21661 gtggcataac tcatgcggac attttctggt gccccaaagc cttcgccagt cacaagcgct
    21721 acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata tttacacatg
    21781 cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt ttcaagagaa
    21841 acacccggca gttctgccac aagcggatat aacgtattca ggcgttcttc aaaggcgttc
    21901 cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc atattgactg
    21961 actgctgtcg gattactggt tgactgggaa gcaacagcaa tcattccatt gatgatctca
    22021 gaattcccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt agacacgcca
    22081 ttgatgatga tggtctgatt tttgattgct tctgaaatcg ttgcaattgg agtaaactca
    22141 ttgccattat aaactaaacg gccataaata tcatcagcaa ctatcaaaat atcatttttt
    22201 actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc agtcggattc
    22261 gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc tagctgctca
    22321 acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag tccttctgcc
    22381 agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac ttcatcttgc
    22441 gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc tgttacgact
    22501 gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac gattgcctgt
    22561 cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccatt gcggattgct
    22621 tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc cacagtcaaa
    22681 ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc tgccaaagtg
    22741 acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat tcctttacct
    22801 tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc aggtaataat
    22861 tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca tcatacatcc
    22921 ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc tgttttgctt
    22981 cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca gatttaggaa
    23041 tgataacggc gatttctttt cctttatcat tcgtacccgt caatgaaaag tacgttttct
    23101 ttcgagtaaa ccagtaaaaa tgatcggctt cctcgagatt tgcatatttc tttgcgattt
    23161 ctactgcttc ttttttcgcc tggaccgccg gacgactgga acgcaagtaa aaaatcgagc
    23221 tgatcacgat gaccgctaat aaaaatacga tcaagccaag caacacgcga ttaatcgttt
    23281 tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt ccttttcatt
    23341 atagcagatt ttaatgatga aaaaagacgg cacctttaac ttccttcttc atttttcttt
    23401 aagaattcat ggatatcctg atagatttca tctatagcta gctcttttat cggtactttt
    23461 ttcggtaatg catggatgat tcgtgcacca tattttgcag tgactaatct ccgatccaaa
    23521 ataagtaata cgccacgatc tgtctccgtc cttatcaaac gacctaatgc ttggcgtaac
    23581 ttcaaagctg ctttaggcac ggattcctga taaaaaggat tgatcccctc tgattccaga
    23641 taagcattcc tagctttgac catcggacgt aacgggttgt cgaagggtaa acgtgtgact
    23701 acaacaatct gcaaagcatc tccaggtaaa tctacgcctt cccaaaagct gtcagcacca
    23761 aataagacgc ttttttctga caaaatgaag cgtttcaaaa gtttttcccg actcccgcca
    23821 aatccttgag caagaatttc acgtcctttt tctagcatag acaaatgcat tttctgatat
    23881 accttttgca aaacatcatg agaagtgaat aaaacaagga tcggccgatc ttcttgttca
    23941 atcaatcctg tgagtgctgc ttctaaataa tcgatatatg cttgattgct gacttgatgg
    24001 actgcaggtg cttctttcgg cacatagatt cttgcctgtt tctcataatc ataaggagaa
    24061 gcaatcactt taagaggtgc tgcttcaatg ccaagtcgtt tagagagata ctgtcgatta
    24121 ggtccgatct tcagtgttcc tccaatataa agaatatggt catatcgagg ataccacaca
    24181 gttgacggca acatagctgc ttgaaaatca aggagatgaa aaatcatctg ctgtgctgta
    24241 ttcccgggaa cgaaccaatg gatcaaatga tctcgccagt cttctagcca ttggtccatc
    24301 agcaagtatt gcttgtctaa ttgatcaaat aagctatata aacttgctaa atcgatccgc
    24361 atctggttgg tccaactttc ttggataatc tcaaaagtca agcgcatctg tgattgtagt
    24421 tgttggatct cttgataaaa aagttgcaga tgatccattg ctgttccaga ttccagtggc
    24481 agctgatcca acatttcttt tgtcatcatg acttcttctg gatattgtcc gttaggttgc
    24541 ttgctgctta ctaagttcat taatccctct gccacgcaaa tctgcgcttc gcatagtgca
    24601 gagagttctt ctcgataaat cgacaaccag cgcaaggctt catgttcctt cggcaaataa
    24661 ctttgtatgc ggtcaaataa ctgattcggc tcttccagtt gctgaatttg tttatggaag
    24721 tagttaatac ttagtcgttt gtcatttact ttttcagcaa tttccggcaa atgatgcact
    24781 tcatcaatca acaaataagg actagctgga agcaatggtt cttttcgctg tgtttcttgt
    24841 actaaaaatg catggttgac tatcaaaaaa ttgctttgtt tgattttctt ttgcaagaaa
    24901 cgcacaaagt cttgtttgta aaatgactga ttccttgcta aaaacccagt gcctctgtgc
    24961 gcgacatctg caaagaatgc atggttcaga ttggttaaat gtaattcatc caaatcaccg
    25021 gtcttcgttt gtgttagcca aactaagaca gccatttggt acaaagcata ttgtttttgt
    25081 cccggcggtt ccacaagtgt acctttaaaa cgctgtaaat caatatagtg gcgatagctt
    25141 ttgataatca caggttgtat cggctgatct agcaattgat tcagtaatgg aatgtctttt
    25201 tccatgatct gctgctgcaa aacaagcgaa acggtgctga tgacagctgg tttttcaggt
    25261 gtagccaaat aactcaaagg caacagataa ccaatcgttt tgcccattcc tgtagctgct
    25321 tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagacagc attcatcagc
    25381 ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt tgcttgtttt
    25441 tttcttggat atgtccgctc gccataatgg acggatgtga atagctccac ttcttttttc
    25501 tgcaaagcta gcccatcgat gatttctaat gagagatcaa ggggctccgg gttttctttc
    25561 atttcttcaa gcacgtggta aatgaaacga ctcgtatcca ttcctgtatg gctacttaat
    25621 cgtgcaatct gctctaatgt gatgatcggc aatttcctca ttcgttcttc gatcagtaaa
    25681 agaagctgac ctgtcacttc ggcatcacta tccgcctgat gaggattctc atgaataaag
    25741 ccaaggcttt ctgacaaatc agctaagcga aaacttggtt ccgtcggcaa gaaaatttgt
    25801 gcaagttcta cggtatcgat gcccggtatc ttcaaactag gaagtccgca tcgcatgagt
    25861 tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat ggtgtctgcc
    25921 aaaagattgt aaatcgtttg ggcgacatct tcaaaataag gcgctttttg gacgcgttga
    25981 ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg atgaatatcg
    26041 atcgaaaaac gtgaagtgat ccgaccagct tcaatcatga cgcagccaaa ttggatgatc
    26101 cgatcttctt ttggattcgt cccagtcgtc tcgatatcaa cgactgcata agttcttggt
    26161 ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata ctacaaagtg
    26221 cagaaaaata ctctcatcgc tttttaagga aaaaaagaca aaaaagccca gacacataaa
    26281 tgtctggact tttattttta atataggtaa attaagcttt agtaacgttt gttgcttgag
    26341 gtccgcgttg accttcttca acgtcgaaag ttactgcttg gccttcttct aaagttttga
    26401 atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt tctgcagtga
    26461 tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc ataataaaaa
    26521 tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat ctggaagatg
    26581 tttatttgaa acaagctacc acagattacc gaacaaaaac tacattctta ctatatcata
    26641 ccgtttacaa aattgccaga gtttttcctt atttttttgt atttattctc ttatttagaa
    26701 gatatatttg caaataaacg aacgtt
//
LOCUS       DAZAJZ010000034        26329 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000034 DAZAJZ010000000
VERSION     DAZAJZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.034"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..359
                     /locus_tag="ACG86D_001357"
     CDS             63..359
                     /locus_tag="ACG86D_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN3140506.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1003..1956)
                     /locus_tag="ACG86D_001358"
     CDS             complement(1003..1956)
                     /locus_tag="ACG86D_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3140507.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1979..2734)
                     /locus_tag="ACG86D_001359"
     CDS             complement(1979..2734)
                     /locus_tag="ACG86D_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140508.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2731..3690)
                     /locus_tag="ACG86D_001360"
     CDS             complement(2731..3690)
                     /locus_tag="ACG86D_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3140509.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3683..4633)
                     /locus_tag="ACG86D_001361"
     CDS             complement(3683..4633)
                     /locus_tag="ACG86D_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3140510.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4783..5235
                     /locus_tag="ACG86D_001362"
     CDS             4783..5235
                     /locus_tag="ACG86D_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN3140511.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5324..7591)
                     /gene="gshAB"
                     /locus_tag="ACG86D_001363"
     CDS             complement(5324..7591)
                     /gene="gshAB"
                     /locus_tag="ACG86D_001363"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN3140512.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7778..8776)
                     /gene="ftsY"
                     /locus_tag="ACG86D_001364"
     CDS             complement(7778..8776)
                     /gene="ftsY"
                     /locus_tag="ACG86D_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN3140513.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8787..9596)
                     /locus_tag="ACG86D_001365"
     CDS             complement(8787..9596)
                     /locus_tag="ACG86D_001365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3140514.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9605..13186)
                     /gene="smc"
                     /locus_tag="ACG86D_001366"
     CDS             complement(9605..13186)
                     /gene="smc"
                     /locus_tag="ACG86D_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN3140515.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13206..13892)
                     /gene="rnc"
                     /locus_tag="ACG86D_001367"
     CDS             complement(13206..13892)
                     /gene="rnc"
                     /locus_tag="ACG86D_001367"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN3140516.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14240..16021)
                     /locus_tag="ACG86D_001368"
     CDS             complement(14240..16021)
                     /locus_tag="ACG86D_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3140517.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16047..16961)
                     /locus_tag="ACG86D_001369"
     CDS             complement(16047..16961)
                     /locus_tag="ACG86D_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140518.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16988..17950)
                     /gene="opp4B"
                     /locus_tag="ACG86D_001370"
     CDS             complement(16988..17950)
                     /gene="opp4B"
                     /locus_tag="ACG86D_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN3140519.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17950..18894)
                     /locus_tag="ACG86D_001371"
     CDS             complement(17950..18894)
                     /locus_tag="ACG86D_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140520.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18895..19899)
                     /locus_tag="ACG86D_001372"
     CDS             complement(18895..19899)
                     /locus_tag="ACG86D_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140521.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20167..20409)
                     /gene="acpP"
                     /locus_tag="ACG86D_001373"
     CDS             complement(20167..20409)
                     /gene="acpP"
                     /locus_tag="ACG86D_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN3140522.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20531..21532)
                     /gene="plsX"
                     /locus_tag="ACG86D_001374"
     CDS             complement(20531..21532)
                     /gene="plsX"
                     /locus_tag="ACG86D_001374"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN3140523.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21581..23617)
                     /gene="recG"
                     /locus_tag="ACG86D_001375"
     CDS             complement(21581..23617)
                     /gene="recG"
                     /locus_tag="ACG86D_001375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN3140524.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23734..25416)
                     /locus_tag="ACG86D_001376"
     CDS             complement(23734..25416)
                     /locus_tag="ACG86D_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN3140525.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25468..25830)
                     /locus_tag="ACG86D_001377"
     CDS             complement(25468..25830)
                     /locus_tag="ACG86D_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3140526.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26100..26288
                     /gene="rpmB"
                     /locus_tag="ACG86D_001378"
     CDS             26100..26288
                     /gene="rpmB"
                     /locus_tag="ACG86D_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN3140527.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7252 a         5723 c         4652 g         8702 t
ORIGIN      
        1 ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca
       61 ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt
      121 cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt
      181 ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa
      241 tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag cttcccgccg
      301 atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa
      361 tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt
      421 tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata
      481 gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat ggaacatcgt
      541 ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg gacagaaaat
      601 agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg
      661 tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat ctaagtttcg
      721 ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac
      781 tattttttgc cactccccat tctagaatca acccgtatga tgtagataat gaattcacgt
      841 tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata cgattttacg
      901 gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg taagaaacat
      961 tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa atgcttgatt
     1021 cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat accagacatc
     1081 tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta ccagttcatt
     1141 ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt ctactacaaa
     1201 taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac cgtgagtgga
     1261 tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga aaccgaatct
     1321 agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta gtgctttttt
     1381 gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt cctctaattg
     1441 agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat tttgctggaa
     1501 agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa tttgacttag
     1561 atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct gaagctgatt
     1621 aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt tataggatga
     1681 aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa caccaagagc
     1741 atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat tatttggcac
     1801 ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt gtgtgctgct
     1861 tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta aaccaaatga
     1921 tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa tgttagtttt
     1981 agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc atttcgtata
     2041 attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta aataactgtc
     2101 catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata tcatgaagaa
     2161 caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc atgatttgaa
     2221 ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa tccgtgtctt
     2281 gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg
     2341 attgatccgc tatttcttct aacccaaggt gttccagtga ttcctgaacg atttcctcgt
     2401 cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact aattctttca
     2461 cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt ttggctaatt
     2521 cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct gtatcttttg
     2581 gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt ggaccgataa
     2641 aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact gattttccat
     2701 ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa
     2761 ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg ttgtattcca
     2821 gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa
     2881 gcttccgata aatagctgac catgacgata agtagaaaag acacgataac tgatatttgc
     2941 gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc ctgtcaaaag
     3001 actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga ggtttactgc
     3061 ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata acaggtacca
     3121 gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat tgccaaagct
     3181 tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga cttgtaaaaa
     3241 ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta ataaaaaaag
     3301 attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc cgaccatcag
     3361 cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca ctcctccgat
     3421 aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca atactgaagg
     3481 agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc tgacagctag
     3541 cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa aatgccagtt
     3601 tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa caagcatacc
     3661 aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga agataaagga
     3721 agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa ggaaaaatca
     3781 acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa gcagttacgc
     3841 caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga atgatcactc
     3901 cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat gatactaagc
     3961 caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca aacgattcgc
     4021 ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac aatggaacaa
     4081 cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct tgaagccatg
     4141 aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact gaaccaatga
     4201 tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg aggaacaatc
     4261 gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc
     4321 ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct aaacgcgcac
     4381 tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga
     4441 tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac
     4501 gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct gacaattgta
     4561 cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat gctactagtc
     4621 gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct tattttaagt
     4681 gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg ggaatatgct
     4741 ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca agatgatgta
     4801 aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga agaacagcat
     4861 aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc tgaaatgatc
     4921 caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga tgcttccata
     4981 ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct agcaacgaaa
     5041 aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga ggaattcgga
     5101 ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga aaaaaaatac
     5161 ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg
     5221 gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa agcttttgat
     5281 cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat
     5341 ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa ggataaacat
     5401 gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg
     5461 taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg
     5521 cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga
     5581 tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt
     5641 ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta
     5701 attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt
     5761 cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca gggactcgca
     5821 acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa
     5881 attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat
     5941 cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag
     6001 gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg
     6061 atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta
     6121 ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat
     6181 ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta
     6241 atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt
     6301 ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat
     6361 gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg
     6421 ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag
     6481 aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt tctctacctt
     6541 cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg
     6601 taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac atgtacatca
     6661 ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca taaggattca
     6721 aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct
     6781 ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt
     6841 cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat
     6901 ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt tcttgcggat
     6961 gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga
     7021 tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct gttttgaatt
     7081 cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa
     7141 attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat ttttttgcta
     7201 aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg atctcttcgt
     7261 ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag
     7321 aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg
     7381 taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat
     7441 aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa
     7501 cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga
     7561 catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa
     7621 ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt
     7681 ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct
     7741 gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt
     7801 tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac
     7861 caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc
     7921 cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga
     7981 attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca agaagtgttt
     8041 cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca
     8101 tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct
     8161 ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct cctgcattgc
     8221 cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag
     8281 cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg
     8341 caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg
     8401 ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga
     8461 gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga
     8521 gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa cctacatctg
     8581 caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg
     8641 caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt
     8701 acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt
     8761 tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat cactttggct
     8821 acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac gaaaggcaca
     8881 gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc attttcctca
     8941 tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa taaagaaata
     9001 ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct tgttttgatg
     9061 atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc taattcttct
     9121 tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc gcttatatcc
     9181 gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt ccttggggca
     9241 ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc caattgctga
     9301 gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc catgatttca
     9361 ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc accttcatct
     9421 accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc tgtacataga
     9481 acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc atctgaaatc
     9541 agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt gatcattttc
     9601 gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc atccgtacag
     9661 aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca tctgctgcga
     9721 ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg aaatcgctca
     9781 agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc tcatctaaga
     9841 tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg gctgtaagtg
     9901 ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga ggttgtactt
     9961 caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat tcagctttac
    10021 cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct tcaaatactt
    10081 ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac aattggtttt
    10141 tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt
    10201 cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt tcttttaagg
    10261 atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct tgggaagctt
    10321 tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct aaaatcattt
    10381 cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct ttttgctcac
    10441 gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt ctatctcgct
    10501 gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat aattcgttaa
    10561 tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct gtcaatgtcg
    10621 ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg actcgggcac
    10681 cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga tctgcttgtt
    10741 cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg ctttcttgac
    10801 ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt
    10861 ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct
    10921 caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg atgttttgta
    10981 gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt
    11041 cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt ttttcttgtg
    11101 cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt
    11161 gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg gatcctccgg
    11221 cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc gtttgcgcga
    11281 ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg ttttgaacca
    11341 cagtttggat ttgatcggga taactgactt gttcactggc aatccctata aacccttcta
    11401 cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt gtcgtcagtg
    11461 gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct tgccgagcat
    11521 ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct tcgatagcta
    11581 acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata cccgaaagct
    11641 gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag ttttcttgga
    11701 tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt aattgataca
    11761 ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt tggatcattt
    11821 ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt tgtgtcaatt
    11881 cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc ttggctaacg
    11941 tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc agttcgtttc
    12001 ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct gccaataatt
    12061 ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga tttgcttcag
    12121 ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct tgaagctttt
    12181 caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt
    12241 tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc tgcttcagtt
    12301 tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg gcttgcgcat
    12361 tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca gatagtttgc
    12421 ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg
    12481 tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct tttagccgta
    12541 aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct tccaattcgt
    12601 ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt
    12661 tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc ccacgacggt
    12721 cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt gagaacgatt
    12781 cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg cagctttgtt
    12841 tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca ctgatctcat
    12901 tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact tctgcaatat
    12961 tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt ccgcctcgca
    13021 gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata ttgctttttc
    13081 cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg actgtcttgt
    13141 ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct ttccctcact
    13201 tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa ttttttcgat
    13261 ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca aaatactcgt
    13321 tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc acctgatttt
    13381 tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc acctgctttg
    13441 actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga
    13501 tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct
    13561 cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca ttctttggca
    13621 aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct
    13681 ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa aacggcatct
    13741 cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc attcacatag
    13801 gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt
    13861 tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat ggatattttt
    13921 tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg tcaaaaggta
    13981 agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg tttagtttcg
    14041 agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa tttgacttaa
    14101 taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc agcgccttga
    14161 cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga catcgccctt
    14221 tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca tttgcccatg
    14281 ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact ggcaatactt
    14341 cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt tgccattttt
    14401 gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata tcgttgatca
    14461 attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt ggtccccata
    14521 gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca tcaatttcag
    14581 gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga cctgttgtgt
    14641 attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc aatggttgcg
    14701 ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca tctttgtctt
    14761 ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt tttttcgctt
    14821 tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg aatactggag
    14881 gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct actgcattat
    14941 tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt gaatctggat
    15001 tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct agataagtat
    15061 aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag gtatcgtaag
    15121 tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct tcagttgcac
    15181 ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca ttccagtagt
    15241 gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg taataagggc
    15301 caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg attgctacac
    15361 cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat tgaagcatgc
    15421 ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt ttgattccgg
    15481 agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta cggaaagtgt
    15541 cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg aaaatcacat
    15601 cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact ttgattgtcg
    15661 cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg attctgaaat
    15721 cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca tcatatgaat
    15781 cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg accgcagctt
    15841 ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga aaagttaatt
    15901 cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta cctccgcccc
    15961 cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc gttttcttca
    16021 ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc tgcagaacgc
    16081 ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag gattgatgcg
    16141 ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata accgatcaat
    16201 gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa agttgtctcg
    16261 ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga acttagattc
    16321 ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt tgaggcactg
    16381 acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc tttagctacc
    16441 caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga aataatcgat
    16501 acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac gatccgcatc
    16561 aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc aaccccgatg
    16621 atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc acctatgatc
    16681 aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt ttgtccttgt
    16741 gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat cactttgtct
    16801 tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag caaacctaat
    16861 gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat ccccatagga
    16921 ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt ctcctttctg
    16981 attggtttta ttgaatccga atacgcgggt caacgatact catgatgata tctgaaagta
    17041 atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc acggtatagt
    17101 cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca aaaatctttt
    17161 cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga gaagccatcg
    17221 gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt acccctttgg
    17281 aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt cgcagatatt
    17341 ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga atatatttca
    17401 aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt gatccacttg
    17461 ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg aagataaata
    17521 aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag attgaatttt
    17581 gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg acaactgtca
    17641 agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca acagagcgtt
    17701 tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa cggaagtact
    17761 ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc actgccggat
    17821 cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg gctaataaga
    17881 agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt cgcagaatcg
    17941 ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag ttgtgatcgt
    18001 acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt ggatatactc
    18061 ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat cgatttgcgg
    18121 aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa tgtcttctct
    18181 tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc acatgtgttt
    18241 tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt gctggattcg
    18301 tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata caggttcatc
    18361 ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac gttgacgttg
    18421 tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc caacgatatc
    18481 tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa aatttcggat
    18541 gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca ttgaatcttg
    18601 gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc gcttcgttac
    18661 atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca caacggcttt
    18721 tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct ttcctttttc
    18781 gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat tgaagaatcc
    18841 actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg tcatctacac
    18901 ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta ccaagtggtt
    18961 cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta tccatggaat
    19021 acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa cgacaccttc
    19081 gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca gtaaagagcg
    19141 tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt caacgaactg
    19201 tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta agtcatgagt
    19261 gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat caagaatctg
    19321 tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga ttggaggttt
    19381 gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata gttcatgggg
    19441 atattggcgt ccaactctcg caggattagg aatccctact tgttctagca attctagtgc
    19501 acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt ctttgatctg
    19561 gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa tcattccgat
    19621 atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat tcagatcgtt
    19681 atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca taatcgtagt
    19741 agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct cgtttttgtc
    19801 taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc ggaatcctgt
    19861 atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc tctctgcttc
    19921 ctaaatggaa gtttttctta ataaaaaagg acattacaca gaataaagcg ttttataaca
    19981 aaggttattc ttatctttgt gtaatgaatt attaataatt atatagaaaa tcgctttact
    20041 ttgcaacata atttctgttt atcgccagaa ttatcagaaa aatgactttt acgtgtcaaa
    20101 aagattaaaa aaagaaggat aaaaagctgt atgcagcttt ttatccttct ttgcttttat
    20161 ttgaaattat ttaagatggc tgctgatata atctactgcg ccaccgactg tttcaatttg
    20221 ttcagcgtct tcatctgaaa tttccgtacc gaattcatct tcaagctcta aaacgaattc
    20281 cataatacta attgaatcag cttttaaatc atctttgatg ttcaattcat ccgtcacttt
    20341 atcggtatct atctcaaaat gatttgaaat aattttcgct actttgttaa atacttcttc
    20401 gcgagtcaac tttttcacct ccaagaatca tacacttgtc aattacctac accatggcta
    20461 attttactgg catttttcag gaatgtccac ctttttttca atggaacgga ttgcttcaga
    20521 tatccaaata ctattcttcg cttttttcaa attgttcgac taattgtcca actacatctg
    20581 tctcaagcat tgtgtggatt tggcggatcg tatagcgaac agcatcgggg cctgttgctc
    20641 catgagtttt aatgactggt gctttcaagc caaacaaaac ggcaccgcca tgtttggaat
    20701 aatccatttc tgctttcaaa gaacgtaagc cgtcttttaa aagcatagcg cccatttttc
    20761 ctttgatccc ttcatttaga atagcagatt ttaatagatt catcatattc attgctgttc
    20821 cttcaataga tttcaaaaca gcgttgcctg taaatccatc tgtgaccact acatcagcca
    20881 caccattgag tagctcacga gcttctacgt ttccaataaa attcaagctt gtttcattct
    20941 gaaggagttc ataggctttt ttcgtcactt cactaccttt tgttgcttct gttccgttgt
    21001 ttaagagtgc gacacgagga cgtccgatac ctcggacttt acgtgcataa aaagaaccta
    21061 aaataccgta ttgcaacaaa tgttctggtt tgttttctgc gttagcgcca agatccagca
    21121 tatcgaaacc ggcaccttct ttccctacta ctggcaaggt agacatcaag ccagggcgtt
    21181 cgatattttt gatacgtcct acgatgaaca accctgctgc tagtagcgcg cctgtattcc
    21241 ctgctgaaaa tacagcatct gcttcaccgt ttttcactgc ttgtgcagca aggaccattg
    21301 atgcagtctt ttttcgtcga attgctttta ctggctcatc atcactattg attttttcgt
    21361 ctgtatggat aatcgtgatg tttttttcat ttgttaagta tttcttgatt tctgcttctt
    21421 ttccgtaaag taagaattct acttctggaa aatcttgctg cgctaaagcc acgccttcta
    21481 caattgcttg aggtgcgtga tctccgccca ttgcatctac ggcaattttc attgtttcca
    21541 ctcctttttc cgtcatcagt ataaagtata gcacagtctg ctaatcgaaa taacgattat
    21601 cctgattttc tatttggagg ctttgcgcta aaggctgata ctcaggaagc atctgccaat
    21661 cctttatctg ccaaagggct gcagcctctt ctctggctac ctccaaaaca tttccatccg
    21721 tcaccaagtc agcaatcgca aattgaggca gaccagattg acggaagcca aatacttcac
    21781 caggaccacg taattccaga tctttttcgc ttagtacaaa accattggtg gtctctgtca
    21841 tgattttcat ccgttccaca cctagttcat ttttaggatt agcaactaag atacagtagg
    21901 aagcatcact tccgcgtcca acacgtccac gaagttgatg cagctgtgcc aatccaaatc
    21961 gatccgcatc catgatcagc atgacggtcg cgttcggcac gtttacccct acttcaatta
    22021 cagtagtcga aactaatatc tgcatctggt tgtctttgaa ggcttccatg attacttctt
    22081 tttcctgatt cttcatttta ccatgtaaca agccaacttc atattttggt gcaaatagtt
    22141 cacgaagttt ttcatagatt tccactgcat tttttacatc taatgcttct gatccttcaa
    22201 tcagcggaca gatgacatac atttgatgtc ctcttgccag ttcttttttc gtccaatcca
    22261 gtacactgtc caattgaggt gttcttaccc agcgtgtttc gatcgggata cgtccagctg
    22321 gaagttcatc aatgatagat acatccatct caccatatgc tgtaatagct aatgttctag
    22381 gaatcggagt cgctgtcata aataagacat ctggtcgcca gcctttttca cgaagtactt
    22441 tccgctggtt cacgccaaaa cggtgctgct catccgtaat aactagacct aaattctgaa
    22501 attcgactcc atcttggatc aatgcatgtg tgccgatcac gatgttaatt tccccttgct
    22561 ctagtttttc aaggagctca cgacgttctt ttgctttcgt tgaccccgta agcaatgcga
    22621 tggttacttc attgggatca aacagctgtt gcaagctttc catatgttgt tgtgccaaga
    22681 tttctgtagg caccatcaac gcaccttgga aacctgctgt aacggtagcg tataacgcaa
    22741 tcgcggcaac cactgttttt ccgctaccta catctccctg caaaagtcgc tgcatatgct
    22801 gcggactgcg aagatcccga cagatttcat tcgttacacg tttttgtgca tttgtcagtt
    22861 caaaaggtag cttttgagta aattctttca atttttggac atcataaagg atctccaaac
    22921 cattcgtttc tgctttttct gcttttttca atccttgaag acgcatttga aataaaaaga
    22981 actcttcaaa aacgacacgt cgtttggctt gatggttttc ttcgtggtct tttggaaaat
    23041 gcatacttct tacagcagaa gctcggtcaa gcagtcgata tttctccaca aggttgtttg
    23101 gcagattttc ttcaatcaat gtgccgtatt cttgaaaccc ttttttgatt agatcaacta
    23161 atgtcgtttg acgaattgat ttgttgacgt gatatatcgg cgaaaagtca tccattgttt
    23221 gagaacctaa aatcttcatc ccgttcaatg ctttacgctt tgcatcccat ttaccgtata
    23281 cggcgatttc ctccgataaa atgacttttt cttttagata aggttgatta aaaaaagaaa
    23341 cgttgaacac atcatgttct tgcatcatac gaaattgcaa tcggcttttt ttataaccaa
    23401 atcgattcat cacaggaggc gagactacga ttcctttcaa agtaaccttt tcttgatctt
    23461 ggatctcgtt tagatttctt tcctgaatat cttcgtagcg gaaaggataa taagtcagta
    23521 agtcttcaat cgtttggatg cccaagctgg caagactagc tgctcttttt tctccgactc
    23581 ctgccagcat agtgattgga tcatctaaag aacgcataaa tttttcctcc ctcccttaac
    23641 aaaaaaagga cttggtcagc gatcatacct gatcactaac cgaaggcctt ttatgaatga
    23701 atactgtgtt agaaagatat tctgacacag aaattattca gcagaaaata gataaggata
    23761 gacaggctga tctccttggt ggatctcgat ttccaaatca ggatgttttt ctgttagttt
    23821 ctcagcaaat tcatttgctt gttcaactgt tccttcatca cctgccaaga tcgttacgat
    23881 ctctgtgtcc tcattgatca tttgttctag tgtacttaac gctgttgctg tgatatctgg
    23941 gttagagatc aggatttttc catcgatcat gcccaaaaag tctccttctt tgatctctac
    24001 accgtcaatt gaggtatctc tgatcgcgtg agtgacttga ccgctcacaa cgctagacaa
    24061 catttctgtc atcgtttctt tgttttcttc taaagaagct tgatcattga atgaaagcat
    24121 agctgtcaat ccttgggaaa tcgttcttga aggaacaact gctactggga tatctgctac
    24181 ttctgctgct tgatcagctg ccatgaagat gttcttattg tttggcaaga caatcacttt
    24241 atctgcgttt acttcttgaa tcgctttaac aatgtcttcc gtacttggat tcattgtttg
    24301 cccaccactg ataacataat tagcgcctaa actttcaaac agctcttgga cgcctttacc
    24361 agcagcgatc gctacgatac catacgggat acgtggtgtt tcttttttcg caaactcgtt
    24421 cacttcttca tcgtgttcca aaatcgtctc atgctgcaag cgcatgttgt cgactttgat
    24481 tttgactaat gaaccgaatt tttgtccata attcaagact tctcctgggt tttctgtatg
    24541 cacgtggact ttgatgatct cgtcatcatt gaccacaagt aaagaatctc ctataccatc
    24601 taaatagtta cggaattctt cgtaatcgaa tgtactatcg actgtaggtc cttctccgat
    24661 ttttaccatg atttctgtac agtaaccaaa atggatatct tctgtcgcca tttgaccttg
    24721 gacactgcga tgatgttctg cattgaccat atcatccatt tctgctggag aaggttcata
    24781 tgtttcatcc gcttggaatt ctccattcaa tgcacttaaa aagccctcat aaacaaacag
    24841 cagtccttgc cctccactgt ctacgacacc gacttctttt aggactggaa gcaagtctgg
    24901 agttttagct aaagcacgct ttgccccttt tacaacagct gtcattactt caacgacatc
    24961 atcagtactt tttgctttct tttctccagc tttcgctgct tctctagcta ctgtcaaaat
    25021 cgttccttct actggtttca ttactgcttt atacgcagtt tcaactccat gagtgaaggc
    25081 tgcagcaaga tcggaagcat ttaatgagac aacatctgga atttgcttag aaaatccacg
    25141 aaacaattga gacaaaataa cgccggaatt tccgcgcgca cccatcagca atccttttga
    25201 aagcattgct gctaattctc ctactttttc tgaggttgaa tctactactg ccttagctcc
    25261 actagtcata gatagattca tatttgttcc cgtatcgcca tctggcacag ggaatacatt
    25321 taatgaattg acgtattctg catttacttg cagacgattc gcacctgcct ggaccatttc
    25381 ctggaactgg cttgcactta tttctgttac actcacctag ataatcctcc ttcagatcga
    25441 ctgctgagtt accagccagt ctcctgccta gtctggcagt acacgaacac cttgtacaaa
    25501 gacattaact gaattggcag ttacgcctaa taatgcctct agattgtatt tcactttttc
    25561 ttggacgttg cgggaaactt cagagatttt cgtaccatag ctgataatcg tataaacatc
    25621 tacagcaata ccattatctt cttggcggac aactactcct cttgagtaat tttctttacg
    25681 caaaatctca gtaatattat ctttaatttg atttttgctg gccattccta caacaccgaa
    25741 cacatctgtc gcggcgccac caacaactgt tgcaattaca tcattggtaa tttcaatggt
    25801 tccagctgac gtttgaattt ttacagccat gaatgagcct ccttaacggg taaaaaactc
    25861 ccatttttct tctatcatat tttatcatag cgcaacttga aataaaaggc agttacccaa
    25921 agtactcaaa aaccttgcaa aatttcaggt ataaccttgt ttaatttaca attattcttc
    25981 tcaattgtca attgtatcta tgaaaatgta cttgcaaatt tccattctat atgatatgtt
    26041 agtctggtat gagctgaatt attctattgc tccaaatcaa ggcaaaggag gaaattacca
    26101 tggcaaaagt ttgttacttt acaggtcgca agacaaaaag tggaaacaac cgttcacacg
    26161 ctatgaactc aacaaaacgt actgttaaac ctaacttaca aaaagttcgc gttatggtcg
    26221 acggaaaacc taaaaaagtt tgggtgtcaa ctcgtgcttt gaaatcagga aaagttgaac
    26281 gcgtttaatt gaatgatact tggaccgttc agatagtgaa cggtttttt
//
LOCUS       DAZAJZ010000035        26234 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000035 DAZAJZ010000000
VERSION     DAZAJZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.035"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..250
                     /locus_tag="ACG86D_001379"
     CDS             <1..250
                     /locus_tag="ACG86D_001379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140528.1"
                     /translation="NNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFP
                     YQRKSLTPDEKSKCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            316..1644
                     /locus_tag="ACG86D_001380"
     CDS             316..1644
                     /locus_tag="ACG86D_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3140529.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            1544..2299
                     /locus_tag="ACG86D_001381"
     CDS             1544..2299
                     /locus_tag="ACG86D_001381"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN3140530.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            2303..3514
                     /locus_tag="ACG86D_001382"
     CDS             2303..3514
                     /locus_tag="ACG86D_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3140531.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            3525..3836
                     /locus_tag="ACG86D_001383"
     CDS             3525..3836
                     /locus_tag="ACG86D_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3140532.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            3833..4171
                     /locus_tag="ACG86D_001384"
     CDS             3833..4171
                     /locus_tag="ACG86D_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3140533.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            4164..4595
                     /locus_tag="ACG86D_001385"
     CDS             4164..4595
                     /locus_tag="ACG86D_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN3140534.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            4592..4933
                     /locus_tag="ACG86D_001386"
     CDS             4592..4933
                     /locus_tag="ACG86D_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140535.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            4936..5526
                     /locus_tag="ACG86D_001387"
     CDS             4936..5526
                     /locus_tag="ACG86D_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN3140536.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            5536..5916
                     /locus_tag="ACG86D_001388"
     CDS             5536..5916
                     /locus_tag="ACG86D_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140537.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            5964..6104
                     /locus_tag="ACG86D_001389"
     CDS             5964..6104
                     /locus_tag="ACG86D_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140538.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            6624..8684
                     /locus_tag="ACG86D_001390"
     CDS             6624..8684
                     /locus_tag="ACG86D_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3140539.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            8755..9156
                     /locus_tag="ACG86D_001391"
     CDS             8755..9156
                     /locus_tag="ACG86D_001391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140540.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            9233..9925
                     /locus_tag="ACG86D_001392"
     CDS             9233..9925
                     /locus_tag="ACG86D_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3140541.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            9922..13812
                     /locus_tag="ACG86D_001393"
     CDS             9922..13812
                     /locus_tag="ACG86D_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN3140542.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            13822..13989
                     /locus_tag="ACG86D_001394"
     CDS             13822..13989
                     /locus_tag="ACG86D_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140543.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            13976..14383
                     /locus_tag="ACG86D_001395"
     CDS             13976..14383
                     /locus_tag="ACG86D_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFN3140544.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            14384..14803
                     /locus_tag="ACG86D_001396"
     CDS             14384..14803
                     /locus_tag="ACG86D_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFN3140545.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            14793..15701
                     /locus_tag="ACG86D_001397"
     CDS             14793..15701
                     /locus_tag="ACG86D_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN3140546.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            15817..16854
                     /locus_tag="ACG86D_001398"
     CDS             15817..16854
                     /locus_tag="ACG86D_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN3140547.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(16915..17220)
                     /locus_tag="ACG86D_001399"
     CDS             complement(16915..17220)
                     /locus_tag="ACG86D_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140548.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            17515..17688
                     /locus_tag="ACG86D_001400"
     CDS             17515..17688
                     /locus_tag="ACG86D_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFN3140549.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            17749..19302
                     /locus_tag="ACG86D_001401"
     CDS             17749..19302
                     /locus_tag="ACG86D_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3140550.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            19350..19715
                     /locus_tag="ACG86D_001402"
     CDS             19350..19715
                     /locus_tag="ACG86D_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3140551.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            19717..21267
                     /locus_tag="ACG86D_001403"
     CDS             19717..21267
                     /locus_tag="ACG86D_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3140552.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            21390..25913
                     /locus_tag="ACG86D_001404"
     CDS             21390..25913
                     /locus_tag="ACG86D_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN3140553.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTN
                     INYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIE
                     LLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIF
                     EIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAE
                     HYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPEL
                     SVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDI
                     LIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRN
                     SANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPEN
                     GNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQAS
                     SYLFKFKQKRSDG"
     gene            26048..26176
                     /locus_tag="ACG86D_001405"
     CDS             26048..26176
                     /locus_tag="ACG86D_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN3140554.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         8593 a         4553 c         5875 g         7213 t
ORIGIN      
        1 taataataaa gactatttct ctaaattcaa agattggaaa agttttatag aactaaatta
       61 tttacaagat ttcttcgatg atgaagatta tatgacgttt aaaaaacttg caccaagtag
      121 ttcattcttt ccttatcaaa gaaaatcctt aacacctgac gaaaaatcga aatgtttaga
      181 ggaagtaaat acctttttaa ataatgcaat tgagataatt ttgtcaagag gaagtcggtt
      241 ttatatctaa tgtgaacaca gcatctacaa atcgtaggtg cttttttgat actcaaattt
      301 tgaaaggagc aatcaatggg cataataaaa tcactattca aatcacgcga taaaccaaca
      361 aatcaaacca tcatgaatcc ataccgattt atgtttggcg gctcatcagc tggtaaaagc
      421 gtgaccgaac aaacagctat gcagatgacg gctgtgtact catgtgtgcg cgtgctatca
      481 gaagccgtgg caggattgcc attacacttt taccaatacc gagatgctgg cggcaaagat
      541 aaagcaatga aacattcgtt gtatttcttg ctgcatgatg aaccaaattc ggaaatgaca
      601 tcatttgtat ttcgtgaaac cttaatgact cacctgctac tttggggtaa cgcctatgcg
      661 caaattatcc gaaatggcaa aggtgaagtt gtagctttgt atcctttaat gccagataag
      721 atgacggtta atcgcgatga caacaaggaa atctattatt tatatcaagc tgatgtcggg
      781 caagtcagac tttcaaaagc tgacgtgctt cacattcctg gccttggctt tgatggcttg
      841 gttggttatt caccaattgc catggctaag aatgctatcg ggcttgcgat tgcaacagag
      901 gaatatggtt ctaaattttt tgccaatggt gccaatcctg gtggtgtcct agaacatcct
      961 ggaactttga aagacccagg aaagcttcgt gaaagttgga atgcgacatt tggtggcagt
     1021 tcaaatgcgg ccaaggttgc agtacttgaa gaaggcatga agtatacgcc tatttccatg
     1081 tcaccagaac aagcccagtt ccttgaaaca cgtaagtttc aactggaaga aatcgcacgg
     1141 atattccgag tgccccttca catggttggc gacttagacc acgcgacctt ctcaaacgtt
     1201 gaacatttga gtttggattt tgtgaagttc tcattatcac catggctcaa tcgttgggaa
     1261 caagcactta ataaatcact gttaagtcgc gatgaaaaag aaaagtattt cataaagttt
     1321 aatgtggatg gcttattgcg tggtgactat gccacacgtc aacaaggcta tgcaaccggt
     1381 attcaaaatg ggttcctatc tcctgatgat gtgcgtgaac ttgaggactt gaacccaatc
     1441 cctggtggtg ctggtgcgaa atattacttc aatggcaata tgctgccaat tgaacaagcc
     1501 ggggcagctt atggcgtaaa caaagaagaa agcgaggaac aagatgaaga agttttggaa
     1561 gtggaagaat caaacggttc tgaatcaaga caaccaagag gaaatcctag agagaaccct
     1621 ctacctcaac ggaccaatcg ctgaggagag ctggtttgat gatgaagtca caccgcagat
     1681 tttcaaagat gagttaaatt caggtggagg taacgttaca gtctggatta attcgggtgg
     1741 tggcgattgt gtcgctgccg caagtattta caacatgctt gctgactaca aaggtaaagt
     1801 cacagtcaag attgacggca tcgccgcttc ggccgcaagt gttattgcaa tggcaggtga
     1861 caaggtactc atgagtcctg tggcgatggt gatgattcac aatccgtcag cggttgcgtt
     1921 tggcgaaaaa gttgatatgg agaaagccat tgccatgctt gatgaagtga aagcaagtat
     1981 tatcaacgcc tatgaacgta aaacgaattt atcgcgtgtg caactggcac atatgatgga
     2041 tgcggagacg tggatggacg caaataaagc tgttcaccta ggatttgccg atgaaatcat
     2101 cggtgagatt gacccggata ttccggttaa taattcgatg tactcggaag tcaaagtaac
     2161 taattcactg acttcagcga ttgctaagaa gtgtcgtatt gaaccacctg aaccaaagga
     2221 acgagaagaa attaataaca ctatcccagc cgattcgtta atgagtcggc ttaatttaat
     2281 gaaaaattgg aggaactaac ccatgaacaa aattcaagaa ttgatggaaa aacgtgcaca
     2341 agcttggaac ggagcaaagg cttttgtaga agcaaagcaa gataaagatg gcttgatgtc
     2401 tgaagaagat gccaagactt atgcggaaat ggaaacaaaa gtgaagaact ttacgatgga
     2461 aattgaacgt atgcagtcaa tggaagcgat ggatcgtgaa cttgaaaagc caatgagcca
     2521 accattaact tcgcaaccta tgactactga aaataaaatg gaacctaaga aaacaggacg
     2581 tgcttcagct gaatataaag aaggtgtact tgaagccttg cgctcaaact tccgacatgt
     2641 ttcaaatgtt cttcaagaag gtgtggatac ggcaggcggt tatttggtgc cagaagaata
     2701 tgactcacgt ttgattgacg gcatgacttc tgaaaacatc atgcgtacac ttgcgacaac
     2761 aattacgaca agtggtgaac acaagattaa tattgcaggt tctaaaccag ccgcttcatg
     2821 gattgaagaa ggtggcgagt tgagctttgg ggatgcaacg ttcgatcaaa tcttgcttga
     2881 tgctcacaaa cttcatgtgg caatcaaggt gactgatgaa ttgctttatg acaacgcctt
     2941 taaccttgaa aactatatca tcgaccaatt caccaaagcc ttagcaaacg cggaagaaga
     3001 tgcgttcttg aatggtgacg gtgttggtaa accactcggt atttttgcag caacaggtgg
     3061 cggacaagtt gccattacaa ctaatactca aagtgcagtt acggcagatg atgtggtgaa
     3121 ccttgtgtat gctttgaaac gtccatatcg taaaaacgcg aagtttatca tgaacgacca
     3181 aaccatcagc ttgcttcgta agttgaaaga caacaatggt gcctacatgt ggcagccagc
     3241 tttggtagca ggtgaacctg accgtctgct cggttatcca gtttatactt cggagtttgt
     3301 tccaaccgtt aaggcaggtg cgccagtcat tgcttttggt gatttcagct actataacat
     3361 tggcgaccgt ggcgtgcgtt cattcgacca gttgcgtgaa ctctttgcag gtaatggcat
     3421 ggttggtttc ttagctaaag aacgtgtgga cggtaaactt gtgttacctg aagcagtgca
     3481 aatcttgaag atgaaagcat ctgcctcatc ttaaggagta gcctatgagt gacttactaa
     3541 ataaggtcaa ggcaaacctc atacttgagc ataacgaaga tgatgagctg ttggaacgat
     3601 tgattctgac cgctctttct tatgctgaga gctatcaaca tattgagtct ggctactatt
     3661 cagaacatga aatgccgcca accactgaac aggcggttat tgtgttagcc agtaactttt
     3721 acgaaagccg tgatggttca actggtggct tctttgcgga taacgtcaat gccagtaagc
     3781 aagtctggga aacggtcaat ctgttactca ggctcgatag gcggtggcaa gtatgagttt
     3841 tggaaagatg aatcaacgaa tacaaatcgt taaagtgacg aaccaaaagg atagcgctgg
     3901 attttcagtg aaacaagatg aagttcttgc agctgtccat gcatacaaag aggaaaagaa
     3961 tgcgactgag aaatgggtaa acatggcgac ttttagttca gcgacctgcc tgtttcggat
     4021 tcgttatatt cctaatctag aggttacgac agagatggtt gtcatttcag atcagaaaag
     4081 gtatcagatt gtgtcggtgg agaatgtccg aaatcgcaat atgtaccttg aaatgctgac
     4141 tactgaaatg gaggtgagtg cggatggcta aagcattgat gaagatgcca gaagatttct
     4201 tgttaaaggt ttcaaaacta gaaaacaaga cagatgaaat catccccaag gttttagaaa
     4261 gtggtgctga agtggtggaa ggcaaagtcc gaagtaactt aactgccgtt gttggctcaa
     4321 atacaaaagt accaagtcgt tcaactggtg aacttgaatc agcccttggt atcacacaag
     4381 ctcgtcaaga taaaagcggc aactggaaca tcaaggttgg ttttgatgag cctcgaatcg
     4441 atggcagttc aaatgctaag attgcgaaca ttctcgaata tggaaaacac ggtcagccag
     4501 caaagccatt cttgaaacct gcaaaatcac aaagtcgcaa gtcatgcatt gaaacaatga
     4561 aatcaaaaat ggaaagtgag gtaaacggca tttgaacatt ttagaggaat tgaacagtct
     4621 ttttgccaca cttcatattc ctgttgaaac gggcgagttt agtgataaag cgccagacaa
     4681 gtattgtgtg ctgacaccac tcgctgacag gttcgatgtt tacggggata actttccaac
     4741 cgttgatgta caggaagttc gcatttcctt gtttgtgaaa ggcaactatc tgaacacgca
     4801 aaaacaactg gttcagaact tgttgaaaca cgagtttacg attactgaac gattatttgt
     4861 aggatttgaa aaagatacaa attactatca tgtggcgatt gatgtcgcca agcattatga
     4921 aatggaggtc tgaaaatggc aacgattgga ttggataaat tatattatgc accgattaca
     4981 gagtcggcaa ctactggcga agaaacatac ggcacacccg ctatgcttgc gaaagcaatc
     5041 tcagctgagc tttcggtgga acttgctgaa gcaactttat acgccgatga tggtgcatca
     5101 gaagtagtga aagaatttaa aaatggaaag ttaacgctcg gtgttgatga tattggacgt
     5161 gctgtggcgg cagtgcttgt tggtgcaacc gtggatcaaa acggtgtctt aatttcgaca
     5221 accgaagatg gtggcacacc agttgcgatt ggtttccgtg ctcgtaaatc gaatggcaaa
     5281 tacaaatatt tgtggttgta ccgagtgaag tttgccgtgc ccagcacaag cctagcaaca
     5341 aaaggtgacg acattacatt ctcaacacca acaattgagg gtacagtcat gcgtcgtaac
     5401 aagttagatg ctagtggtaa gcatccatgg aaagcagaag ttgatgaaaa cgatacggga
     5461 gttgaacaaa caactatcaa tggatggtac acgagtgttt atgaaccaga tttcacagca
     5521 gactaggagg caacaatgag tgaagaacgc agtgccaaga ttagccttgg cggagatgaa
     5581 tatgaactga ttttgacaac aaaagccacg aaagaaatcg ccagtcacta cggtgggctt
     5641 gataaccttg gtgagaaatt gttgaaatcc gagaactttg aattggcgct tgatgaaatc
     5701 atctggttga ttacgctgtt ggcgaatcag tccatcaaga tttacaactt gaagaacaca
     5761 gaaaagaaag atgaactcac gactgaatat gtggagttga tgacgagtcc gttgcagttg
     5821 gctgaatata aatcagcaat cactgaagca atgttcaagg gcacagctcg caatatcgaa
     5881 agtgaaacag attcaaaaaa caaagcaggc gagtaagtga tgaagaaaca tttactcgcc
     5941 tgtattacta tgggacagtt caaatgggta tgacgtcaga tgatttctgg tactgctcgc
     6001 ttggactgtt tttggattta tgggagtgtc ataagcaatt tactgggatt agcaagccga
     6061 aagttgagtt attcattgat gatgtgatac cgagtggaat ttaagggaag gtggtgattt
     6121 atttggcaga taattttggt ttaaagattg gcgttgaagg tgaaaaggag tttaagaact
     6181 cgttgcgtga aatcaaccaa tcattcaagg tgctaggttc agaaatgaaa ctggtatcat
     6241 ccgaatttga taaaaacgat aaaagcatca gctcactgtc tgctcgcaac caagtcttaa
     6301 acaaatcgat tgatgcccag aaagaaaaaa tcaccaccct tgaaaatgct ttgaagaacg
     6361 ctagtgacag ttttggtgaa aacgataagc gtactcagaa ttggacagtg caattaaaca
     6421 atgcaaaagc ccaactcaat aatatggagc gtgaagttgc taacaacgag aaggcaattg
     6481 acgccatggg tactgaagaa gttgatgctg ctaagaaaac tgatgagctt ggcaacgaac
     6541 tcaaggatac aggtgacgag gctgaaaagt ctggaagtaa atttgaaaag ttcggtggcg
     6601 ttctaaaagg tatcggggta gcaatgggta cagtggcact tgctgctggc gctgctgccg
     6661 tggagctagg aaaacaagtg gtatctgctt acgcggacta tgaacaatta gtcggtgggg
     6721 tggatactct tttcaaggat gcgtcatcca ccgttcaaaa gaatgccgac aatgctttca
     6781 aaactgccgg aatgtcagcc aatgaataca tggaaactgt cacaggtttc tcagctgcct
     6841 tgatttcatc tctcggtggt gataccaaga aagccgcagg agttgcggat caagctgtta
     6901 ctgatatgag cgataatgca aacaagatgg ggacggatat tgagtccatc cagaacgcct
     6961 atcaaggttt cgccaagcaa aattacggga tgctggacaa cttgaaatta ggttttgggg
     7021 gcacgaagtc cgaaatggaa cgtttgctcc aagaggctga gaagatttct ggtgtcaaat
     7081 atgatatctc gaacttcgct gatgtgactg aagcgattca cgttattcaa accgaaatgg
     7141 gcatcactgg tacaaccgcc aaggaagcga gtgagacgat aagtggttcg attgacggta
     7201 tgaagtctgc aatctcaaac ttgatggctg gacttggtaa cagcagcgct gacattggta
     7261 tgttgattgg caatgtggtg gaacaatttc aaaacgtcat caagaatatt ctaccggtca
     7321 ttcaaaatat cgtgtcggct ttaccggcag tggtggatgc cattattcaa gcattggggc
     7381 agttattgcc aacactctta gaagcagtaa cctcattatt ctcacaagtg cttgaaacga
     7441 tacttggctt acttcccaca cttattccag tggtggttca agccgtttta acgattgttc
     7501 aaacacttat tgagaactta cctttactga ttgacgctgc ctttcaactt gtgacaacac
     7561 ttatccaagg actaggcgaa gcgctgccag aacttattcc aattacagta caggcaatca
     7621 taacgattgt gaacggcttg attgaaaact tgccattact gttggatgct gctttacaac
     7681 ttatcatggg acttgcccaa ggacttatca cagcacttcc aatactgatt gaaagtctgc
     7741 cacagattat tacaggaatc gtgaacttta ttatcagtgc tattccacaa atcattcaga
     7801 ctggtattca acttttgaca tcacttgtga cagctttgcc ggatatcatc aatgcgattg
     7861 ttgcagctat tccagtgatt attcaaaata tcatcaatgc cgtgattggg gcaatccctc
     7921 agttgattga cgcaggtatt cagttacttg tggcattgat tggtgcgtta ccgcaaatta
     7981 tcacaacgat tgcgaatgcc atgccgcaaa ttatcaacgg aataacagga actttggtaa
     8041 acaacatcga caaaatcatc aatgctggtg tgaagttgct tgtggcatta gttcaaaact
     8101 taccagcaat catttctgcc attgtcaaag cagtaccgca aatcatcaca gggcttgcga
     8161 atgctttcgg tgagtatgtt tcaactatgg gaactatcgg gaagaacttg attacaggat
     8221 tgtggaatgg tatcgctgat gctggaagtt ggttacgcaa caagatttca ggattctttg
     8281 gcggtgtagt agacaacatc aaggacttct ttggtattca ttcaccatca acactgtttc
     8341 gtgatgaaat cgggaagtac atggcacaag ggattggcgt aggttttgat aaggaaatga
     8401 ctggtgtttc taaaacgatg cagaaagcta ttccaacaaa actttatatg ccaagctttg
     8461 atattgatac cgatatccac gctgccgttg atagcacatt ctcgatgctt gatttaggtg
     8521 ctaaggttga tgccttaaca aacatcacga gtgaaatgtt gccaacgcta cttaaagcaa
     8581 tggacattaa agtcgtcttg aatgatggaa cattggtcgg caaacttgct ccagaaattg
     8641 ataagagctt ggcgacgttg aatcgtagac gtgtagcata ttagtgttgg attttcacat
     8701 tttgactgta tactataact aacgagtgtt tactgaagag atggtggtaa tcatatgttg
     8761 aagcataaat ttaaagcaaa gaaacctgca ctgataggat atattctact gcttgctatt
     8821 ggattatttt taacggcagt aagatggtat agcggcataa atcatggttt tgtattcatc
     8881 aacaatcaga ttaattctca catttcaaac ttcgcattaa gctttctttt ctatttggtg
     8941 ataggctatc tttggttgct tatgggaacg aatttaaggg ttcttactgc gatgggagct
     9001 ggtctgattg ttgcaaatat tgtatgtgaa acgatgatga cttttctgaa tacaccagat
     9061 ctaatcgatg caatatatgg aatttccggc gtgctggttg tatacgcgta tttagcgatt
     9121 accaagaaaa tagggctgga agaaacaacc gaataggatg atagattata aactttagga
     9181 ttagacattg ttgtctggtc cttttttagt tatttagatg ggcggtggtc aattgaacgc
     9241 atttatttta gacggaacaa ttaattcacg gactgattta ggtttgcgaa ttacggaagt
     9301 gccagttatt ccagtgacgg aacgtgtcat tgatagtatt gagattgatg gacgtgaagg
     9361 agatttgacc attctgaaag gctggaaaga tgtgactttc ggttttaagg tagtgatttg
     9421 gaaatctgat gtttggacta gctggcagat aattttgcca caaatcctaa acgctaaaac
     9481 tatcgcattt tcaaatgaac caaacgtgtt ttacccaatc aagtatgcta aggcgagcgg
     9541 actaactcag attttatctt ccatgtggga gtttgaaatg gagttaacct gttcgccttt
     9601 tcgttataag accaatgtag cgactatcaa tcgaaccacg tcaggaacgg tcaataatcc
     9661 cggcaacatt tactcattgc cgaagattaa ggtctacggc aatggaacta ggacactcac
     9721 gataaatggc aagcctatcg tgttaaatct gacgaatggc aacctgacac tagattcgga
     9781 tttgaaagaa tgcttttacg gggatgtggc agccaatcaa tacatgacag gtgactttcc
     9841 tgagtttcga gtcggcagta atactgtgac tcttggtaca ggtataacca aagtagaaat
     9901 agaaccgagg tggcgatact tatgattacg ctttacaaca aaacagaatc aaatttcaat
     9961 cataacggtc tcgctgtcct tgatggcaac attctgaacc ccgtggtgag tgaggaattg
    10021 aacggactct tttcctttga attcgattat ccaattcatg ctccacacag tgaggaattg
    10081 ttgccagaaa tgattgtgaa agcaccagta ccagaactgt ccgaccagct tttcaggatt
    10141 gttgaacgag atgatgcact tggtggtttt ttgcacatag tagctcatca tattttctat
    10201 gacctagcca caaactttat tgaagatact tatatcgtca acaagaacgg gtcgggagct
    10261 ttgacacaac tgcttggtgc aactcaattc atgcaccgtt ttaccggtac atcaaacatc
    10321 agtgcggtca ataatgtccg cttggtgcga ctaaatcctg tagaggtgtt gcttgatagt
    10381 gacctagaga atggttttca aagcagttat ggtggtgaaa ttgtacgcga taacttcacg
    10441 atttcaatgt taacgactcg tggcagtaac aacggcgtac aaatccgaga caagaagaat
    10501 ctgacaggat ataaagctga tgttgattac tctggaattg ttactcgcat tatgccacaa
    10561 gggtatgatg gactatttct tcccgaaaaa tacgtggaca gtccaaacat caaccaatac
    10621 gtccagccta aaatcaaagt catcaagtac gacaacgtca aagttggtga tgaagaggat
    10681 gagtttactc aagccgaggc ctatgatgaa cttcgcagat tggctgggtt agaatactca
    10741 gctaacaagg tcgatttgcc aaaagccacc tatgatgtgg agtttgcccc gcttgagcga
    10801 actgaagaat ataaagattt tgcacagctt gaaacgatta atttgggtga tactgtttca
    10861 gttattcatg ccgaagatgg attgaacatc acagctagaa tggtctcata caaatacgat
    10921 ccatttctga aagcgtatat ctcagtaaca ctcggaaaca tcacgccaaa attcacggat
    10981 gtggcgaagg acatcaaccg ggtggacaac aaggcggagc aagccaaaga cgatgccaat
    11041 tacgcattga gttcggcaaa tggtaaaaac accaacttct atggcccgac aactccaaat
    11101 aatccgaaac tcggtgacgt ttggtacaaa gaaaatggtg acaagtttga aatgtgggtg
    11161 tatgaaatcc gtgatggcgt cactcagtgg tatccactta tgacagatat gacggcagaa
    11221 gaagtaaagc aagccgtgga acaagcacaa aaggatgcca atactgccac tgaaaaggcc
    11281 aatcaagcat ttaacgatgc tgtgaatgct ttgaatgcag ctgaaagtgt aggtgcaagt
    11341 gttgaaacgc ttactacatc cgttgaacag aatactagca agatttcatt agtggcgagt
    11401 ggtatgtcaa ctcttgaagg acgagtaatc acagctgaat caactctgac agtgcaagct
    11461 gggcagattg ctagtaaggc aagtcaatcg acggttgata atctgacagg gcgtattaca
    11521 actgcagaaa gcaccatcac ccaaaatgct gatagatttg agctatctct ttctaaaaca
    11581 aataaagcgg tcaatgtttt agccggtatg agcattgcag atttgcagtg ggcagttggt
    11641 ggtctgaata caaatgatgg aaaagaaaat acctatggtg gatacgttcg ctcaggtttt
    11701 acaagagtta gaaatggcga aaagtatcta cttcaaacat ttgtgggtgg gaaactttac
    11761 tctacttacg caacggctta tctgctttat tacaaagagg ataagtcctt tttatcgtac
    11821 acccaatttg gaaacaccac cacaccgctt actgttccaa gcagtgccgc ttttattcgc
    11881 attcgtttaa caactagtga tgcaccagaa accatcagtt gctatatgtt gaaaacggat
    11941 gtgacaaatg gctatgttga tttatccgag ataagtaacg tcgtaaaact tacagcgacc
    12001 actgatgcct tactgttaag tgtttcagaa accaaagaag caatcggcgt tccatttaaa
    12061 gtcaatcaat gggaacaagg aagtttgagt accactgacg gttcagaagt gtccgcaacc
    12121 aattatctcc gaagtgagtt ccttgatgtc aaagtgggag ataagtatat cgggcaactg
    12181 cgtgacggaa ctcaagctgc tttctactat cattactacg gcatggattt accttacatt
    12241 gattttgtcc caacggcaaa acaatttagt gaaaccctaa cggcgggaac ttatactaat
    12301 gcgaccattg cgagttatct agtaagtaag aacgttgaat cgatgagtat tacaagtggc
    12361 gtgacaacaa acccctattc aaattcagga gattatctgg tcatttacaa attgccattg
    12421 gaaggcaaag tcgctccgag tgtaatttcg tggaatggtc gcaaggatac atcagataat
    12481 attgtcttgc tttatacagg tggtgcttgg gaacagattt cgaccgttac aagtagcagc
    12541 aatgccactt attcttggac gttaacggct gagcagatat cagggattac tactgacagc
    12601 gtgtatctag ctttcttttc agttaagact ggcacatatg ctgggattta taattcaagc
    12661 aacacacctt ttactctaaa cccgtcggat ggttcaagtt atcatcaaat ctcttatact
    12721 ggttcgagcg gagcaattac agtcccgagt ggcacattga aaatgcgagt ccgagtgaac
    12781 acgagcatca aacccgatga atatgaagga aatatttatc ctacgaccac aaggcaagac
    12841 tataccaatg ccaacacatt atattcagca attctcatgc agaaagactt aatcaattta
    12901 cgagttggta aaggagacgt gattaatcaa atcaatatct cacctgaaag tattttgatt
    12961 gctggaaaca aggtgcacat cactggtcaa acaaccattg atagtgcagt gattagttct
    13021 gccatgatcc aaaatgcggc catcacaaac gcaaagattg cagatgccac aattcaaagt
    13081 gcaaaaattg caagcattga tgcggcgaag attaccacag gaactttgtc ggcagcccgt
    13141 attggcgcga actcgattac ggctgataag attgctgcta atatcctaac agcattgact
    13201 gcgagttcgt ccattcgaat cacaggaact acgattggat attattcagg aagcactctt
    13261 gtgactgaaa taaactcgca gggaatgacc attcgacgtg gtggaacgac agtcggaaca
    13321 attggtgcaa ataacatcag tggtcactct gattggcgtg gattggtatt tgatcttgag
    13381 tatggaacag agtatatgtc atgggctcat aaagattcct cctctgcgag tgtctacacc
    13441 cagaagcttt cttggtatgc gagaacattg cagtctggta tggagaaggg gttccacttt
    13501 gacgacgaca tttatttacc atcaatgctt cgtataggga cacaacatca aatatctgtt
    13561 ggtgggatta catttagtag ttccctttat actaagattg gctcaagttc cggcagcgct
    13621 ggtattgcat tcaacggttc taatctgatg ctcggagatg atgggaagtg ggttgacttt
    13681 ggagtcatcc gtgaaatctg tgccaaactt gcgggacgaa caatcgcttt accaaccggt
    13741 ttcagtagca acggtacagc gagtggttgg tacaatccgc agtcgtttaa ttcaatgacc
    13801 acatacagtt aggaggccgc catgaacgaa gaaaaagaag aagtgatagt aactgaacct
    13861 atcgaggcag atattccaaa gccaatgaaa gttgggacgg atatggttga tttgcttgtg
    13921 atggtcgtgg atgatctttg tgtccagatt ggtgatttaa aggagaagat agataatgct
    13981 caaaattaga aatgaagatt tggtggacgt gatgagtttc ttaaaaggtt ttgacttacc
    14041 accaaagatt agccgagtgc gaacgaagct tgcaaaactc atccaggtga aggttgatga
    14101 actctatcaa gacgaggtag acttgcttca aaaatacgga aagaaagatg agcacggcaa
    14161 tttggtccaa catgacggtg ccttttcact catcccagaa accgcaactg aatatcacaa
    14221 agaaaaggca gacttgctca acgaagaaac ccacatcaac gtggatgaac ttcaagacaa
    14281 gctgccttta ttggttgctg gatttgaaaa tagtgaagtc accatttcag ggaaagatgc
    14341 tgaaatgctc gatttaattt tagaacaatt ggaggatttg taaatgaaag aagtgtggaa
    14401 ttggattcaa ctcgccatcg caggtggtgg aggagtgatt ggatggtatg tgggaggaat
    14461 tgatggtttg atttatacgc ttgtaacttt tgtggtagtg gactatgtaa ctggtctgct
    14521 tcgtgcaatc aacgaaaaga aactatccag caagattggg gcaaagggca tcactaagaa
    14581 aatcatgatt tttcttttgg ttggtgtggc tcatatgctt gatgtggaag tcatcaaagg
    14641 tggaaatgta ctgcgtgatg cggtcatttt cttctatatc tcaaacgaag gaatttcact
    14701 gctggagaat gccgtggcga ttggactgcc agtgccggaa aagttgcgtg aaatcttgca
    14761 gcagttacac gaaaagaagg aggaaacaga aaatgggaat tagtaatcta gcaacaaaat
    14821 atggatttca gtcatttagc cattactcga atggtcgtag tggtgccaaa gtagataaga
    14881 tagtgattca tcacatggca accaccaact atgatattgt tcctgatatt tggaaaacgc
    14941 gtgaggcgtc agcacactac ggtattggaa aaaatggaga aattcgagca tacgtggacg
    15001 aaggaaacac agcttggcac gctggtaatt ggaacgccaa tatctcaagt atcgggattg
    15061 agcactgcaa tagcactgga tcaccgaact ggggtatcaa tcaagcaact attgatgcaa
    15121 gtgcaaagtt gtgtgcagat attgccaaac gttatggtct agggaaattg gtggtgaaca
    15181 agaatatctt ccctcactca catttttcgg ccacatcatg tccgggacag ttgcttggta
    15241 aactgcagga gattgcggat aaagccaatg cgattaataa tggttcatca accccaagca
    15301 cgccagctag tggtctgtat cgtgttcgca aatcatgggc tgacgccaag tcacaaattg
    15361 gtgcctataa ggacttgaac aatgcgaagc gggctgcaga tagccattct ggttataagg
    15421 tgttcgacca gaacggtaat caagtttatc catctgggtc atcaacatcc aacgtgaaaa
    15481 ctcttgtggt tcaagtgagt ggtctcaacg ttcgcaacaa gccaagttta agtggtgcgg
    15541 ttgttggttc atacaataaa ggcgcaactt ggacgcttcc gaagtcagag aagatgacga
    15601 ttgcggatgg ttgggtatgg gctcgttgcc cgattggata ttgtgctgtc ggtaagaata
    15661 ctggtaagac ggaatctgac gattatattt tgatttcata aatttaatcg gaacaaaatt
    15721 atgtccggat tgttgactta aaatatcgaa cgtgttatta ttaattcaac cagaacataa
    15781 atttgttccg attgaattat agaaagaggt atcgatatgg ttacgtttaa agatttgtta
    15841 ccagaaccaa cagaaagcca acaagctttc attttagatc acgggaaaac agaagatgat
    15901 ggtcagttga aatacgctga tgatgctaaa agttacggtt ggaacatgcg ccaatatggt
    15961 aaattgaaag ccggcgcggt tgttttgaat cgtcatccgg ggaaaattac taaagaccgt
    16021 aagtgggaaa tttacggggg cggatacgtt gagagcattt ctgaacctga tgaagctgga
    16081 aatgttacgg cagtaattac acacgcattt acaatcgaac cggcaattaa acaaggtgat
    16141 tcatttattg aaaacttcga ttggaataca cctagcaaaa agaagcgtaa aaagccgaat
    16201 tcatgggcct acttctggga tcagtatgga atgaatgaaa ttacctatgc cgattttgtt
    16261 gcattgattg aaaatcgtca tcttatccca atcgatgata cacaatcgtt gccgtctgaa
    16321 ctggatctta caaacgagga agttgaagaa attgagcaag aaagttcaaa aggatttact
    16381 gttcttgttg atgaagtggg accaaatcgc acgaatggga ctcagaaacg aaaattcact
    16441 ggacgccata ctgattggga gcgtgtcaac aaggcaaaac aaaaaacggg tgctctaggc
    16501 gaagaaatcg tacttgattt cctcattcag gaagcagaaa agaacaaaac aaaacttcct
    16561 gaacatgttt ctaaaactga aggagatggt catggctatg atatttgtgc gtttgaccaa
    16621 tcaggtaatg aaatccatat tgaagtgaaa gcttcaaaaa caaacttctc tgacggattt
    16681 gaaatgagta ctaatgaggt tgctgcctcc cttggagata caccatataa aatttacttt
    16741 gttcacgacc ttgatgtggc gtcaaaagtt tgcaaaatca aaatttatga tggcccaatt
    16801 actgaagaaa acttcacgat ggttccaacg agctacaaaa ttttcaataa gtagtttgac
    16861 gtctttataa ttcaaaacag cctcacaagt tttattgtgg ggcctttttt tcgtctagtt
    16921 ttctttaagg taggtgttta cagcattttg cattaggatt aaatgatttc ttaactgaaa
    16981 tacctgctct ttgtaattga tgcagaaaag aggcgattct attgattcca tacgattatt
    17041 catcgcgttt atattggctt gtaaaaactg gtataaagct tcttcagcct ctatggtttt
    17101 aaagcacttt tcaaattctt gtttgaagct ttcatcaaat tcctccagtt cctttgttgt
    17161 cgcagatttg caaaaatgtt catattcttt ttctaaccct tccatatcaa aactactcat
    17221 aactttctcc ttcccacaat tgatatttat attgttattt attggatgaa gcttgtaaag
    17281 gcttatttga tgtttgggaa gtaaaaagtc aaaatttaca aagaataatt cctactatat
    17341 ataaacaaac ttagaagcct gcaagagtga ttcgtttcat tcttgcaggc ttttttcgtt
    17401 ttgtgggttc gagtcggtta tttctttcgc atatggatga aaggcaaagg aaaatttgtg
    17461 attgggttta ctttcatgga ttttcttcgc ctgtatcttg aggaggcgaa agcaatgact
    17521 aacaagaaaa cagaaattaa ctattcagat gaagcagcct attacatttc acagcagatc
    17581 cttctgaact tacttgttac cggtaaaatc aacctggaag aatacgtcaa aatcgacaat
    17641 aaaaaccggg aaacattcaa tcctttttta tcaaggataa tggtctgaaa cgttgatata
    17701 acggcacttt agagctaaca tgtcacctaa tggaaaggag ttgagctgat gaaaacgata
    17761 cataaattgg cacgtccaga gttgccggaa gcttccaaac taaaggtggc agcatacgct
    17821 cgtgtatcaa catcaagtaa tgaacagctg gcaagtttac aaacccagat tactcactat
    17881 gaaaatcaca tcaagaacca tgaacaatgg gactatgtcg gtgtttatta tgatgaaggg
    17941 atttctggaa ccaagactgt aaaacgtgat ggtttaaaac gattgattaa ggatgcagag
    18001 cttggaaaga ttgatttgat tctgacaaag tctatttcca gattttcaag aaatacagtt
    18061 gactgcttga atctggttcg gcagctaact gatattggtg tcaccatctt ttttgaaaag
    18121 gaaaacatta atactggtga tatggaaagc gagttgttgc tttcaatttt atcaagcttg
    18181 gcagaaagtg aatcctattc ccattcagaa aacttgaagt gggcgattcg aaaccggatg
    18241 tctaaaggga aattcaaagc ttacccgccg tatggctaca agcggaaagg ctatgatttt
    18301 cacatcgttg cagctgaagc aaaagttgtt aaacaaatgt tccagtgggc attagatggc
    18361 atttcgactg gaatgattgc caagcggtta aacgatggaa aacaattcac taaacgtggg
    18421 gcaaattggc aagcaacatc agtcagaagt attttgaaaa atgaagttta taccgggact
    18481 ttgacgatgc aacaaacctt tgttgatgac cagtacaaac gacgcataaa caagggcgaa
    18541 ttgccaatgt accagatcaa aaacaatcac gaacccatag tatctaaaga agattacgac
    18601 cgagttcaag aactcattcg gagaaaagct gaggccaaag ggaatactgg tgacgggaaa
    18661 aagtacacca agcgatacgc attttcaggc aacatcatct gtgataaatg cggcagcaaa
    18721 ttcaagcggg ttcacactta ttggaaaggt aatacgaaga aggttcagtg gaagtgtacg
    18781 gggcatctta aaaataaaga tagctgcgat gcattaccaa ttacggatga agatttgaag
    18841 gttgcttatt tgaccatgtt gaataagttg attttcggac acaagttggt tctggaacct
    18901 tttatgaagt ctttgaataa agcaggtgcg aatcaggaag ggctcgaagt gcttgctgat
    18961 gaaatcgaca agattcaaga aaagcttgag gttctggcaa gtttgactgc ttccagtatt
    19021 ttatcaacga aggtgtcttt agaagaacaa agcaaacttc aacaagagag ggctgtcgca
    19081 caagaaaagc agcgcctcat cattgagtca gtcaacggca aaagcactca gcaaattgaa
    19141 ctagaagcct tatacaaatt cgcgcggatg gcagagatgt tgacggactg ggatgaagat
    19201 ttattcaaga agttcacaaa cagcattaca gtttacaacc gccaagaagt cggatttgaa
    19261 cttaagtgcg gactactttt gaaagagagg ttaccagaat gaacaataca attccatacg
    19321 ggtatgtcat taagaatgga gaaatcaacg tggatgaaca gcaagctcaa caaatacgtg
    19381 agttcttcaa tatttacctt gagggtgttg gcttacaaga agctggcaaa cgctctggca
    19441 ttaacaagcc gcatcccttt atgaaacgta ttttgatgaa tgaggtttac ttaggaaatg
    19501 agatatatcc aaaaatcatt gatgaggata ttttcttcca agcggatctg gagcaagaac
    19561 gaagaattaa gttctttaaa aaacgcaagg agatcaaagt gccagatacg aaggtaatac
    19621 ccactcattt taaatctggc aagattaccg aaaagtattc tgacccattc aaacaagcgc
    19681 aatatgcata ttcaaaaatc aaggaggtta agtgaaatgg caggaaaaca ggtgacggtt
    19741 attccaatga agcctagaca atgggcagca gagaataccg aagagaaacc aaagttgaaa
    19801 gtggcagcct attgtcgtgt ttctactgaa atggaggaac aggcgagttc ttacgaagca
    19861 caggttcaac actatacgga ctacattcaa agaaatcccg attgggagct tgtaggaata
    19921 tttgcggatg aagggatttc aggaactggg acgaaaaagc gtgacggttt taaccggatg
    19981 attgaggcgt gtcaaaacgg cgaagttgag tacatcatca caaaatcaat ttcacgcttt
    20041 gcccgtaata cggttgattg cttgcaatac atacgacagt taaaagaact gaagattgca
    20101 gtgttttttg aaaaggaaaa catcaataca atggatgcga agggtgaggt acttttgacc
    20161 attatggcaa gtctggccca gcaagaaagc cagtcactaa gtcaaaacac caagatgggc
    20221 gtacagtatc gtttccagca gggacagcta cgtatcaacc acaatcattt tcttgggtac
    20281 actaaggacg aagatggtaa cttggtgatt gaaccaaagg aagcagaagt catcaaacgc
    20341 atcttccgcg aataccttga gggcagttcc ttgcaagaaa ttgctaatgg tctaatggct
    20401 gatggcatcc taacgggagg gaagcgaaag ctctggcgag gagaaggagt cagattgatt
    20461 cttcgaaacg agaagtacat gggggatgcg ctgcttcaaa aaacatatac taccgatttc
    20521 ttgacaaaga aacgagtgaa gaatgacggg agttacgctc agcagtatta tgttgaagat
    20581 agccatccgg ccattatccc aaaagatatc ttcatgcaag cccagcaaga actagaccgg
    20641 cgtaaatcaa tgaaaaacaa aaagtcacag tgcttttctg ggaagtatgc gttatctggt
    20701 atcacagttt gcggtgactg tggcaatgct taccgcagag ttcattggaa gaatcgagga
    20761 actgtgtggc gatgcaaatc cagagtggat aaacgagaac ataattgcac gggtcgaaca
    20821 atctacgaaa cagacctgca tggagctgtt atcaaggcaa tcaatgaaac gttaatcgat
    20881 cgtgatgctt ttcttcagca attaaatgac aatatcaact cagttatgac tgatggatta
    20941 actgaccgat tggcagaact agacagtcag ttgaaagagc ttgaagctga aattattagc
    21001 ctggcagttg gcggtcaggg ttacgatgaa ttggcaagcc aaatattttc actaagagat
    21061 gaacgagatg cggttgcaaa acaaatagca gcgaatacta atttgcaaca acgggttgat
    21121 gagatggtag tctttgtaaa agaacacgat gtcatcaatg agtataatga agtgcttgtt
    21181 cgaaggttga ttgagaaggt caccattttt gaaaagaaca ttgttgttga tttcaaaagt
    21241 ggggtgagag tgactgtaga aatttaattt gaatagatag gactagacgt taatgtgggt
    21301 agccatatta acgtctttat tgattggatt tcataaagtg attgatgata taatatatta
    21361 tgtaaaaaat aggctggggg cgaagttctg tgagccaatg gaataccatt gatgaattaa
    21421 gggatatttt tattcgtaaa tatacggatt ctgagttgag taatgaactt gaaaaggcct
    21481 gcagcgaaga atatgaattg atgcgtgatt ataatgggcg tcagatttta gagttattac
    21541 agaatgtaga cgatgcctat ggagatataa aatcagaaaa tgatgctagc aatgacgaag
    21601 tgaaagttca gataacatat aaaaataata tcttggaagt tgggaataca ggcacttcat
    21661 tttcaaaaga tacaattgaa agattgtgct taggaagagc gagtaataaa tcatcacaaa
    21721 atatcggaaa taaggggact ggttttcgtt ccttgctaaa cgatgctgag tgggtcgaac
    21781 tgtattcggg agaatttgct attcgattta gtgaagaatt cacaaaaaag ctttttgagc
    21841 agtatgtatc aagagattct gacaaattca gtgaattaat ttttgagcag aagaaaaatt
    21901 ggaaaaaaga agattatgat ttatgttttc caataatgaa ctgtcctcaa ccaataaaca
    21961 aaattgattc tggttttgat acattaattc gagtaaaact aaaagaaact aatttaagta
    22021 aaggcacgag tgtttctaaa caacttcagc agccatttta taagtcactg ttatttttac
    22081 caaatataac aaaaattgtt attgaaacaa ctgatgatga aaaaatatca gagaaaattg
    22141 tggatggaaa tgatgtattg atagaaaaaa gtgatgttac tggtcaatca ccgtctgaag
    22201 aatattttgt ttttgacaaa gaatctatga taggagataa agttgctaaa attgcactag
    22261 ccgtttctaa agattctgat tacgattact ccaaagaaaa actatattgc tactttccag
    22321 tacgtaattt tggtacacca attcatgcac ttataaatgc accgtttata acaaataatt
    22381 cacgtgatga cgtcccggat gatagtgaac aaataaataa gaaaatattt attgaggtgc
    22441 tcaaatttat ttctgaagta gcagaaaaat tatctaagcc tgaatatgaa gacatagcat
    22501 taaaaatggt aactcctttt aaagagaata aactatggga cttagatgta tttgacttaa
    22561 tgaacgaatt cttgggtatt ttatctaatg cccaaatact accgactgtc aattggcaat
    22621 atatttctat caaagataat ccaaaacttt tccttcaaga attccctgag gagctaatag
    22681 gtgagacatt ttccaccttg ttgcaattat tagaaacaaa cgaaaatgac caactagtac
    22741 aagaacttgc taattataat gaatacactg gcctgtcgtt tgttgtagat gaactatctg
    22801 ataaaatcaa cattctttca tcttcttggg aaaatagaat aaggacaaaa gtgttcttat
    22861 ggtggagtga gcaatataaa agttctccga ttgtccctaa gctattaaag gacaatagtg
    22921 acaattggtt gatgaaatca gataaggtat tcttaccgac cgatacaggt gttactgtgc
    22981 ttccaaaaga gctgtcttct tgggttagtt taagcatttt gaaacaaaca tatgttgatg
    23041 agttaattta tcagattaag acttcatcaa gttggaaaaa taaatgggaa gaaacagcta
    23101 atgcatacaa agctgaacgt acaggagata aacgtattct agatgcattc agtgaaaaat
    23161 atcttgcaat tgaattcact gagcaaagca gttcagattt gatcattgga acgattaatc
    23221 gacaaattga cactgttgaa aaatctatca gtttcattaa ttggttttat gataactaca
    23281 atgaaaaact atcagctgga agtgagctgt ctaaagttcc atttaatctt ccaaacgttg
    23341 ctggtgacgt tcaaccttcc aataaaatgt atattggaaa agagtatggc aatgaattag
    23401 gagataagct ttttcaagat acagattaca cggcattgaa aaaaatagat tgctctagtt
    23461 ttgctgagga gagagatttt ctgtcattta ttcttcgatg tggtgtttcc aaatatccta
    23521 agatttatga taacgattct ttaaaaaatg atgttggatt taaaaagttt atttataaca
    23581 aatataagaa tgaaataaaa tttacgaata ttaattattt aacttctaag tacatcagta
    23641 attttgaaga attaatagac aaattagata cgaaagacat tgtcaattgg ctcaatcaag
    23701 acgaagaatt gacagattta ttatcttcgg taaaaaaaga atcaagtgca agtcagcaat
    23761 cgaactggag tggattgtac tttaattcaa atgagtatgt gaaatacatt ttgaataaaa
    23821 ctgcatggat taagattgat ggagaaaaat atagcccgag acaaattgtg ctatatgaaa
    23881 agctccaggc acacgttccg ggattgtatg gagtttctga acaaagctta atggaactct
    23941 tggggcaacg cattgttgaa cagttggatt tcagaaaaaa tatggcgatg ttttcggatg
    24001 atgaaataag agctatatta gttgagttac caaaattcga taaaggcgaa ataagtcgca
    24061 gattatataa tgaacttata agaaataaaa aaggaatgaa tcctacttat tctaccgaag
    24121 gattatcagt cttagccaaa gatggcttct tttatccaaa caaagaatta aggtatgctg
    24181 ataagcgact gcctaaatca atagaaaaac aaatgcgttt tatttacgtg cctgaaaaag
    24241 ctagtacaac tacgatatat gaatggcttg gggttgaacg cttcaaaaca aatttaaaat
    24301 tggaaacatt tgagttattg gaagattatt ctgaggattt caaaaaagag attgatgata
    24361 tcaaaactgc ggtattatct acgatagatg acaatagtaa gaatgttagt tcattaaaac
    24421 gtattcagat tattccatgc agcaaaatat ctgctaaaga tgttgagcaa gatggtcagt
    24481 caatcgtttt ggaagattat tattttgtgg aaagtaaagg aaaattctac ttaaaattac
    24541 ctgctaattc tattgaaatt aatcaattac gtttgattga tacgttttct tcaacaatcg
    24601 tagacatatt caagcaggtg ttaaatctaa gcttagattt gaatttgatt gaattgttaa
    24661 tttcccgaga tacggaacat aaacaaagaa aaataactga agaattcggt gtagataagt
    24721 ggaatgaatc gtttgagcta ttgtttagcg aaaatgcgtt aaataaaaaa gttaatgact
    24781 ttttgttatc aaatggacta aatgaagagg aatatcctac catttttgaa attgactatt
    24841 catatgcatt aaaagaagaa gagtatcaat tgttggcgat agctctagaa agcatttcaa
    24901 aagatattga ggatttgaat ggattgtcag aattgattca tttggatatt cgtgaatttt
    24961 ggaaactgaa attaagatct tatctagata agaaagcgga acattaccgc cagattttat
    25021 atagcaatgc aaataaaagc ggtgatgaaa tactcaaaga aacattccta gaaaaaattg
    25081 atagatacaa agattatcaa tttgagattt ctgaaattcc aaattcagtt caagtcgaca
    25141 ttgagaaaga aagtaagaag agatttccag agctaagtgt gtcaattact gatgaaattg
    25201 acattgatgg cctgtatgat aataatgtcg aagagttagt cacggttgca gctattacaa
    25261 tagatgattt tgattattat attagaggtc atcaaaaagt ttcttcctta ctgtattttg
    25321 gtattccgtc ccgtttaaga gaagatatac tgatattcgt tactaaagaa aatgatgttg
    25381 atttgaacgg agaaaaatct ggtatctcta ttgaaggaac atcaactgtt caaaccattc
    25441 ttgaaccatc gaatagtgtt agtaggaaag gtaagaacac acgaaatgga gaaagaagta
    25501 aacgagatta tgataataga aactccgcaa atgatgatgc tggtgaaaca gcagaacaaa
    25561 ttgcttacgc tgaattgact cagaatcaaa aacttaatgt aatttggaat acgaaatatt
    25621 ctagtaaatc ttcagatcgt aataagcagc caccaaacgg aattgtttgt gatatgtggg
    25681 ttcatgaccc agagaatggg aatatgtact ttgaggtaaa atcatcaatt acagaatttg
    25741 aaatgtcgat taatgaatat aattctatgt tagataaccc tgactcttac gaggtcgttt
    25801 tagtcaatcg tgataccaaa gagataagtc gtcataaatt tgatgaactt gatggattga
    25861 aacaggcaag tagctatctt ttcaaattta agcaaaagag aagtgacggt tgagtgattt
    25921 gtttccacaa acctattcaa tctatcaact caaaaatcat aaacaaccat tattatagta
    25981 ggaagataca gagtgtactc tcaacgcata tcgaattagt cgcttcattc gttaaaggag
    26041 aataaaaatg gaccaaaaag aaaaaatgaa agaattgatc aaaaagaaac aaggtggagg
    26101 ccgatccaaa caaatggaaa cacctaagaa tgatcgtaaa aacatgagaa aaggccctaa
    26161 aatctttaat aaatagacgt gctaaagaaa gactaagaag tgttaacttt tgttattgtt
    26221 tcttagtctt ttta
//
LOCUS       DAZAJZ010000036        25832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000036 DAZAJZ010000000
VERSION     DAZAJZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.036"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..683
                     /locus_tag="ACG86D_001406"
     CDS             51..683
                     /locus_tag="ACG86D_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140555.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(817..942)
                     /locus_tag="ACG86D_001407"
     CDS             complement(817..942)
                     /locus_tag="ACG86D_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN3140556.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1076..1969)
                     /locus_tag="ACG86D_001408"
     CDS             complement(1076..1969)
                     /locus_tag="ACG86D_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN3140557.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2151..3077)
                     /locus_tag="ACG86D_001409"
     CDS             complement(2151..3077)
                     /locus_tag="ACG86D_001409"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN3140558.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3162..3923)
                     /gene="pflA"
                     /locus_tag="ACG86D_001410"
     CDS             complement(3162..3923)
                     /gene="pflA"
                     /locus_tag="ACG86D_001410"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN3140559.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4167..6398)
                     /gene="pflB"
                     /locus_tag="ACG86D_001411"
     CDS             complement(4167..6398)
                     /gene="pflB"
                     /locus_tag="ACG86D_001411"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN3140560.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6675..9125)
                     /gene="parC"
                     /locus_tag="ACG86D_001412"
     CDS             complement(6675..9125)
                     /gene="parC"
                     /locus_tag="ACG86D_001412"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN3140561.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9137..11182)
                     /gene="parE"
                     /locus_tag="ACG86D_001413"
     CDS             complement(9137..11182)
                     /gene="parE"
                     /locus_tag="ACG86D_001413"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN3140562.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11348..11782)
                     /locus_tag="ACG86D_001414"
     CDS             complement(11348..11782)
                     /locus_tag="ACG86D_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN3140563.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11909..12547
                     /gene="plsY"
                     /locus_tag="ACG86D_001415"
     CDS             11909..12547
                     /gene="plsY"
                     /locus_tag="ACG86D_001415"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN3140564.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12679..13554)
                     /locus_tag="ACG86D_001416"
     CDS             complement(12679..13554)
                     /locus_tag="ACG86D_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN3140565.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13636..14427)
                     /gene="codY"
                     /locus_tag="ACG86D_001417"
     CDS             complement(13636..14427)
                     /gene="codY"
                     /locus_tag="ACG86D_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN3140566.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14445..15845)
                     /gene="hslU"
                     /locus_tag="ACG86D_001418"
     CDS             complement(14445..15845)
                     /gene="hslU"
                     /locus_tag="ACG86D_001418"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN3140567.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15859..16407)
                     /gene="hslV"
                     /locus_tag="ACG86D_001419"
     CDS             complement(15859..16407)
                     /gene="hslV"
                     /locus_tag="ACG86D_001419"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN3140568.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16444..17349)
                     /gene="xerC"
                     /locus_tag="ACG86D_001420"
     CDS             complement(16444..17349)
                     /gene="xerC"
                     /locus_tag="ACG86D_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN3140569.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17547..18923)
                     /gene="trmFO"
                     /locus_tag="ACG86D_001421"
     CDS             complement(17547..18923)
                     /gene="trmFO"
                     /locus_tag="ACG86D_001421"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN3140570.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18892..20970)
                     /gene="topA"
                     /locus_tag="ACG86D_001422"
     CDS             complement(18892..20970)
                     /gene="topA"
                     /locus_tag="ACG86D_001422"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN3140571.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21092..21949)
                     /gene="dprA"
                     /locus_tag="ACG86D_001423"
     CDS             complement(21092..21949)
                     /gene="dprA"
                     /locus_tag="ACG86D_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN3140572.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(22005..22772)
                     /locus_tag="ACG86D_001424"
     CDS             complement(22005..22772)
                     /locus_tag="ACG86D_001424"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN3140573.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22765..23625)
                     /gene="ylqF"
                     /locus_tag="ACG86D_001425"
     CDS             complement(22765..23625)
                     /gene="ylqF"
                     /locus_tag="ACG86D_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN3140574.1"
                     /translation="MTIQWFPGHIAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23885..24283
                     /locus_tag="ACG86D_001426"
     CDS             23885..24283
                     /locus_tag="ACG86D_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3140575.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24520..25062
                     /locus_tag="ACG86D_001427"
     CDS             24520..25062
                     /locus_tag="ACG86D_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN3140576.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25145..25696)
                     /gene="lepB"
                     /locus_tag="ACG86D_001428"
     CDS             complement(25145..25696)
                     /gene="lepB"
                     /locus_tag="ACG86D_001428"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3140577.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         6830 a         5462 c         4433 g         9107 t
ORIGIN      
        1 tcaatttgtt attatacttt atatacttat caatttaatt tggagatatt atggaaattc
       61 aacgtagaac cactaatttt ttacatttag gctctttttt tgcaactttg acgatcttat
      121 tttttctatg ttattcatac cttaagacct cacatgcgcc ttctagtgca ttaggctggt
      181 tgcttttgga tcattctgat atcacagaag ccgaagaaaa tccgtttttt ttgattttgt
      241 cttctctttg cggcttactg ttttctgttt atttcggtgc ctattttttt catcgacatc
      301 ctggtacgtt aaacgaagct tcaagcgtat tgattgcatt taaaacgttt tcttttttag
      361 caattctttt ttattcgttt aatcacaata cgcttttctt ctttatcatg aatggtttag
      421 tcgtgatttt atccattttt acttatttta ttttttcact catttttagc caattgaaat
      481 gtccgctctt ctttcgatta gttcctgtca cctgttttat ttacagcttg ttttcctttt
      541 ttgtccttgc tttaatgcca ctgaccaacc cttcgacgat ccaagtcgca ggaaatctgt
      601 tggatatgct cttcatcgtc agtctaggtg tatttatgtg gcaaagaaaa aacaaacaaa
      661 ataaaaagca ttttgaagcc tgactattac gacttcataa atgctttttt agttttaaac
      721 acttcaatct aaaagaataa cccaagattg gataaattat taatgactcg ccattcgttt
      781 cttagtgatt ctggattatc aaatggactt cctaatctaa tcagctagtt cttcccataa
      841 aatgccatac ttgactgaat cataatattg attatttaaa aatctaactt ttcttatcac
      901 tacttccttt gtcattccac atttctcacc atttttttgc attcctttgt ttccagacca
      961 tgtagtgaat ctaataggtg gtagataggc aaaatactca aataattctt ttaaccataa
     1021 aggaagagaa aggctcctcc tgcaagagtc agtataattt ttgcttactg ctctcttagg
     1081 ttttactcac taaaactgtt ccatattctt tgatataaga aaatgcatgc ggtataaatt
     1141 tcttgctcag ctcagaaaag atttgtcgtt taagaaaggc catttcctcc attgtcaatg
     1201 aaggatccaa tgttggtaat tcaatctcaa aattgttttt ttcgacaata ggtaattcta
     1261 atgtgaatga ttcttcttcc tttatagtat tcgtcacaag tagagactga tggaaaatac
     1321 taggccggcg atttttatat ttattcttac gtatcctgtc aacgataact tctatttgat
     1381 caaaaaagta cgattcattt acgtctccaa taagttcggc taattctttg aatgccttcg
     1441 ttacaggaag ctgattcttc tgtacaaaaa agtaattagc caaagtaagc gggaagtgtt
     1501 ctatagagga aagtgtgata aaacggtact tctcatttcc tgcagttgtc ctcagcaatt
     1561 ctgtctctcg gctgtttctt actccataaa aatacgcttc tttccgttca tcttcgaaac
     1621 aatacgccca aataacttct cccatcgcta atttttgttc atcaacagag agtctcttca
     1681 attgttcagc tatggtttca gcagccgaag tggcttgttg caagtaatct ttaggatcaa
     1741 atattggaaa gttcaaatga tatcttcgtt cttccctctt gattaatcct gcatcgatca
     1801 ataaatcaag aaaatgggaa aatttatttt gcgaaaattc ttttttcaat tctcttagaa
     1861 tcgtttcttg ttctcgatgt tcatatagat aatcggtcaa tgaagaaaaa agcggatcat
     1921 cggtcagttt ttgataaaag ttttccttat tttttgaata atagttcata tatgttctct
     1981 tttctatccg ttttatttta cttaaaaggc ttttacgtaa aaaggaggaa gaacaagagt
     2041 cataattgac tgttattctc ctccatttct tcggccttta gtatgttcta tgtttctttt
     2101 tatatcaatc gcttatccat tcattttgaa tgcttcatac taccatttga ttagctaaat
     2161 acagccgtca attgaggtac gacttgtttt ttacgtgaaa cgacaccttt aagcaaagca
     2221 cggttgttgg tcaaagttgt ttggaaagct tcttctactt tttcttttcc tttaccagca
     2281 acaagtaatt ctgaatcact gtctaagatg tttgtgacaa ctaatacgaa cagatcgtag
     2341 ccatttgctt tcatttcatc ttccattgct gcttctagct ctgcttgtcg atccatcact
     2401 tcattaaggt ccactgtatt gacttgagcg atacgaacgt tggaactgcc cattgggaaa
     2461 ctttttgcat ccaaatcaag aagaaccgca gctgatttat cacttagatt tgttcctgct
     2521 ttcaacattt caagtccata agtgtttaga tcgacttcag cgattgctgc aagttctttt
     2581 gcagctgctt catcttcagg cgtacatgtt ggtgatttga acaacaatgt atctgaaatg
     2641 atagctgaga gcatgattcc tgcgatttct ttaggaattg ttacttggtt ttctttatat
     2701 agttttaaaa taatcgtact tgtacatcca actggttctg cacgatagta caatggatta
     2761 gctgtttcaa agtttgcaat acgatggtgg tctactacag ctaaaatatt tacttcgctg
     2821 atatctgaaa tactttgttg gaattcatta tgatccacaa gcatgacatc tgcattttct
     2881 gctttagctg actctactac tcgtggtgca gatagctgaa agtggtctaa tgcgtattgt
     2941 gtttcttcat taggtgtacc tagagcaact gcttctgttt ctttacctaa ttcattttgc
     3001 aagtgtgaaa acgcaatggc tgccccgatt gcatctgtat ctggattttg gtgaccaaaa
     3061 actaaaattt ttgacatgtc attcgctcct ttgtgatttc tcctatttaa taatagcaaa
     3121 aaaacggaaa gaagcaatac ttatcttctt tccgatgcga cctatcgcgt taaatatttt
     3181 gtatactctt ctgtatgaag caatcgttta gcattttcga cacgttcttt tgtaggtggt
     3241 tcgatccctt ctaatggata tttcaaacca agtgtttccc acttgtattt tcccatcgta
     3301 tgataaggaa gaatctctac tctgtctaca tttgacaagg tttgaataaa gttgtctagt
     3361 cggatcaaat attcatcata atcacttcgc tcaggaacta gtacatggcg aatccaaacc
     3421 ggtttgttga tttttgacaa atattgagcc atttctaaaa tattttcatt gtcccaatgc
     3481 gttagttttt tatgctcttc attatcgatg tgtttgatgt caaataacag taaatctgta
     3541 tacttcatca attcttgaaa acgtgaaaag aaaggttctt catacgtaaa aggttttccg
     3601 catgtatcca atgtagtatg catcttccgc tcttttgctt ttttaaatag ttcgatcaaa
     3661 aaatcgatct gcaatagagg ttctccacca ctaacagtaa taccaccttt atctccccag
     3721 taaggtctga agcgttctgc tttatctaaa agttcatctg ctgtgtattc tttccctccg
     3781 ccaatattcc aagtatctgg attgtgacaa aattggcaac gcatgcggca tccttgcata
     3841 aacacgacaa agcgcagtcc aggtccgtct actgaaccga aagtttcgat agaatgaaca
     3901 tagccgattg ttttttcttc catttaagtt cacatctttt ctttgatttc attttaagta
     3961 caagttagct gtcgaagcga agctttctga acaaaatttt tcgcaattgc cgcttcgtta
     4021 tgtgtaaaat ttacagggac ggaacataca agtcctgccc ctgtaaatcg tttctttcaa
     4081 ataagcatac tgattattcg attttgatgc gatggctatt caacgtcctt tcgacgctaa
     4141 gtcgaaaaac ttttttcacg aactttttac attctgtcgt gtgaagttct catcaagaca
     4201 tctaattgtt gttctcttgt caagtcacgg aatttaactg cataaccaga tacccggatc
     4261 gtcaagttcg gatatttcat tggattttcc attgcatcac gcaataaatc attactgaat
     4321 acattgacat tcaaatgata agctcctttt gagaagtatc catctaagac attacgcaag
     4381 ttttcgattc ttgtatcttg ttcttttcca agacctgaag gattgattgt ttgggtattt
     4441 gaaattccat ccaatgcaca gccgtaattc attttagctg ttgagttcag gctagctaat
     4501 aacccacttt gttccgcatt gtaacttgga tttgctcctg gcgcaagtgg ttttccagct
     4561 tcacggccat caggagtatt tcctgtattt ttaccgtaaa caacatttga agtaatcgtc
     4621 aataatgaag ttgttggttt tgagttgcgg taagttttat gacgtttgat ttgtcccatg
     4681 aagtattcca agatccatga aactaattca tctgcacggt catcatcatt tccgtattta
     4741 gggaagtcgc catccacttc aaaatcaact gctattcctt tttcattacg gattgctttt
     4801 acttttgcat atttgatcgc tgacaagctg tctgccgcat gagagatccc agcaatacct
     4861 gtagcaaatg tgcgttgcaa gtcagaatcc atcaaagcca gctgagctgc ttcataagaa
     4921 tatttatcat gcatataatg aatgatgttc aacgtattga catatagttc tgctaaccaa
     4981 tccatcatat tcatgaagtt atgtttcact tcttcaaagt ctagtatttc tgatgtgatt
     5041 ggacgatatt ctggtccaac ttgggcgcca gtcacttcat cgactccgcc attgatggca
     5101 tataatagtg ttttagccaa gttagcacga gcgccaaaga attgcatgtc ttttccgaca
     5161 actgttcctg atacacagca agcaatcgca caatcatcag aaccccattg ttcacgaagc
     5221 tgattatcat tttcgaattg aatcgaagaa ctttcgatag cgattctgct agcatatgtt
     5281 ctgaatcctt ctggaaggtg ttcagaatac aaaacagtca agtttggttc tggtgaaggt
     5341 cccatatttg ttaatgtatg aagcagacgg aaatcattct ttgtaactaa tgaacgacca
     5401 tctaatccca tacctgcaat ggataaagta gcccagatag ggaatcctga gaataattgg
     5461 ttatattcag gtgtacgcgc gaatttgacc atacgtaatt tcattactaa gtggtcgatc
     5521 aattcttgtg cttctttttc tgtgattact ccattatcca aatcacgttg gatatagata
     5581 tcaaggaatg ctgagatacg tccgattgac atcgcagcac cattttgtga tttgattgct
     5641 gctaaatatc cgaagtatag ccattggaca gcttcttttg catttgcagc tggtttagaa
     5701 atatcatagc catatttcgc agccatttct ttcatttgcg ctaatgcacg atattgttca
     5761 gtgatttctt cacgtaaacg aatcacttct tcactcattg tatgataacc agtcatatca
     5821 tgatcttttt tcttttgttc catcaaatag tcgattccat acaatgccac acgacgatag
     5881 tcaccgatga tacgtccgcg accgtaagcg tctggaagcc ctgtaatgat tttgttttta
     5941 cgagcagcac gcatttcagc agtataagcg tcaaatacac cttgattatg tgttttgcgc
     6001 cagtcagtga aaatatgtgt caattcatcg tttggtgtat agccattgct ttttagcgca
     6061 ttgttagcca tattgattcc gccgaacggc atgaatgctt gtttcaaagg tttgtctgtc
     6121 tgtagaccga cgattttttc caaatcttta tccaaatagc ctgcatcgtg tgaagtaata
     6181 gttgatacga tttctgtgtc catatcatag acaccgttac gctcgaattg gatatcattt
     6241 aacttttgca attcatccca taatttttct gttgcttctg ttgggccttc caaaaagctg
     6301 tcatcaccgc gatattcagt gtagttattt tgaataaagt ctctagtgtc tacgccttct
     6361 ttccatttgg tacctttaaa tccattccat tgctccattg tatggacctc ctaaatttct
     6421 gttttatgta acagttttta cgtgattagt ataacacagc accctatctt tgcaagggtt
     6481 ttcttgtttt ttatttaaca atttttatgt tttatttaca tatatttaaa tcaacgttga
     6541 cataccaaca tttaagcatt gtgaacactt tcatcttttt ttgcaaacgt ataaggattt
     6601 attaaaaatg aatgaaaaag agacgccacc tgtattaata cagatggcgt ctcttcatta
     6661 aaatactaca acagttacga ctcgatgaaa atctcaggag catcgataac ttcgatgact
     6721 tctccacctt ttttctcatc catcgcaaat gatccattcg atgttcgatc acctataggg
     6781 aaatcagttg ggtcaatcgt taactgctgc cctttttgat tcatcaaggt tatttctttt
     6841 gatcttcctt ccgacatata gataatacgg tgcggatttt ttttcagctc acgcatgacc
     6901 gtcactcccc gcttcgcacg tgaagtttgg ctgatttctt gagcaagcat gcgtttaaca
     6961 gctcctcgct gagtaacgat tacgatcggc gtatcacctt ctgctaacac aagcagcccg
     7021 ttgataatcc tatctccatc tttcaaattc atagacttca ctccagctgc ctttgctcca
     7081 acaacgggaa catctgttaa aggatagcgc aaacccattc cttgatgcgt tactaagaaa
     7141 acatctaagc ctggctgatc ttctttcaga tacacagcaa tcagttcatc ttcttccgcc
     7201 tttagcttca tacagctgag cggacggctc ttataggtgc gccatggttc gaactctgtc
     7261 attcgtgttt gcttgatcaa tccattcttg ctgatgaata caaatgtttt ctctgcttga
     7321 attttttgat atgggaaaac agctaaaatc gattcgtcta cagacagatt ggagattgtt
     7381 tgagaaatgt gctcaccagc atctttccat ttcaaatcgg gtagttcatg gacagggcgg
     7441 tagatcacat ttgctttatt tgtgatcaga agaacatggt ccaatgtact caattcacct
     7501 gagtatagca agtagtcgcc ttctcgcatc ccgatttctt ctggtttgga agcactataa
     7561 gaacgaaggc ttgttcgctt cacatagcct tctcgcgtca cactgacaat tacgtcttct
     7621 tgtgccacta agacttgcgt atcaattttg atttcttcga tttcgtcttc gattgtcgtt
     7681 agacgagcgg aagaataatt ttttttcact tctcgcaatt cttttttcat aacagagaaa
     7741 agctctttgt catttgaaag gattttattc aactctgtga tttgcgcaat caagctttcg
     7801 gattcctttt gaagctctgt gatatccgtg ttcgtcaaac ggtataattg caatgtgaca
     7861 attgcttctg cttgttcttc tgtaaattga aagacgtcga ctaaattctt tttggcgtct
     7921 tttttgtctt tactttcacg aatcgttgcg attacttcat ccagaatcga taaagctttc
     7981 atcaatcctt ctacgatatg ctgtcttttt tgagctttgg ctaaatcgaa ttgactacgt
     8041 tttgttatga cttgctttcg atgctcgata taacttctta aaatgtcttt cagtccaact
     8101 tgatgcggag tcatatggtc gatagcgacc atattgaaat tgtaattgat ctgtagctca
     8161 gtgtttttga acaaataatt caaaattcct tgagcattgg cgtctttttt caattcgacg
     8221 acaatttgca gacctgttct atcactttca tctcgtactt cagctattcc atcgattttc
     8281 ttattcaaac ggatctcatc catttttttc accaaggtcg ctttatttac ttcatatggg
     8341 atttctgtga tcacgatttg ctgtttatta cccttcattg cctctatctt tgttttcgaa
     8401 cgaagaataa cttttcctcg tcctgtttca taagcttttt tgatttcatc ttttccttgc
     8461 aagatccctc ctgttggaaa gtcaggtcca ggaatatatt ccatcaattt ttcaaggctc
     8521 gcttgtggat gatcgatcat atagattgtg ccatcgatca cttcagctaa gttatgagtc
     8581 gggatttctg ttgcatatcc agcagatata cctgtcgatc cattgacgag aaggtttggg
     8641 tatcgagctg gaaggactgt tggttctttt tctgtatcat caaaattcca cacgagatcg
     8701 actgtttctt tctcgatatc tgctagcatc tctccactta acttggataa tctggcttca
     8761 gtataacgca tcgcagcagg tggatcaccg tccatactcc cgttattccc gtgcatttcg
     8821 atcagtactt cccgcaactt ccagtcttgg ctcatgcgga ccatcgcttc ataaatactg
     8881 atgtcaccat gaggatgata attccccatg atatttccga cagattttgc tgatttacgg
     8941 aatcctttgt catacgtatt gccatctttg ttcatggcaa ataatatccg gcgttgcact
     9001 ggcttcaatc catcacggat atcaggcagt gcacgttctt ggataatata tttggaataa
     9061 cgtccgaagc ggtcacccat cacttcttct aaagttaatt cttgtacttc ttgtcgattt
     9121 tccatcaggt gacctcctat tctacttcaa ataactgatt gttttcattt ttatctgctc
     9181 gttcttcatc taataaatca ttagaaactg aaggagagat ttcagtatcc tcttttttat
     9241 ctaagatact gccatcttct tcgagcgtaa attgcacgtg attttcgatc cattttcttc
     9301 gaggttctac tttgtcaccc atcagtgtgg ttactcgacg ttcagcttgc gcagcatcat
     9361 cgatacggac gcggatcaac gtacgggaag tcggatccat cgttgtttcc cacaattgtt
     9421 ccgcattcat ctctccaagt cctttatatc gctgcagcat atatcctttg ccgacttttt
     9481 taatcattgc tgccaattcg tcatctgtcc acgcatattc aatgacttgc ttctttcctt
     9541 gtcctttgga tactttatat aaaggcggta acgcgatata tactttccca gcttcaatca
     9601 gcggtttcat ataacgataa aagaaagtaa gcagtaatac ttgaatatgg gcgccgtctg
     9661 tatccgcatc ggtcatgatg attactttgt cgtaattaca gtcttcgata gaaaactcag
     9721 gaccgactcc tgcaccaatc gtatagatca tcgtattgat ttcttcattt ttcaagatat
     9781 cttgcatttt agctttttcg gtattgatga cttttcctcg taaaggtaga attgcttgga
     9841 actttctgtc tcttccttgt ttagcagatc cgccagcaga atccccttcg actaaataca
     9901 attcattttt cttagggttt cgggattgtg cgggtgtcaa ttttcctgaa agaagagatt
     9961 cgcctttttt acgcttcttc ccatttcggc tttcttctct ggctttacgt gctgcttctc
    10021 tcgcttctct ggctttgatt gctttacgga ccaacatctg gctcatttcg ctattttcct
    10081 gcaaataaaa gcccatttgt tcactgatga cgttatctac tacggtacga gcaactggcg
    10141 taccaagttt ttcttttgtt tgcccttcaa attgcagaag attttcaggt acacggatag
    10201 aaagtacagc agctaaccct tctctgaaat cactaccttc cagattcttg tcccgttctt
    10261 tcagcaaacc aacttttctc gcatattcat tataagattt cgtcatcgcc gctttcatac
    10321 cagcttcatg ggttccccca tcttttgtcc gaacgttatt gacaaaagat aaaacatttt
    10381 ctgaataacc gtcattgtat tgataagcga cctctacttc gataccttct ttttctcctg
    10441 aaaaataaac aactggtgtc aatgtatctt tttcttcatt cagataatca acaaattctt
    10501 tgattccttc ttcgtaatga aaaatttcct tgactggttc ttctccacga agatcagaaa
    10561 gagagatttt aacgcctttt agaagaaagg cagattctct taaccgttcg gctaaaattt
    10621 catatgaaaa tttcgttgta ctgaatattg tatcatctgg taaaaaatgg acagtcgtcc
    10681 cgtttgcttt ttttgttttt cctatctttt tcaaagtacc gtcaggttta ccgccgtttt
    10741 taaatttctg ctgatactct acgccatctc taacgatcgt tacaattagc catttcgata
    10801 aggcatttac cacactggct cctacaccgt gcagtccacc agaagttttg taccctcctt
    10861 gaccaaactt tccgccagca tgcagcacag taaaaatcac ttcaactgtt ggtattccgg
    10921 aagcatgcat tcctacaggc atcccacgtc cgctatctgc aacagttata ctattgtctt
    10981 catggatggt cacatcaatc tcactgccat aacctgataa tgcttcgtca actgcattat
    11041 cgacgatttc atagactaaa tgatgcaagc ctcggctgtc agtggatcca atgtacatcc
    11101 ctggtctttt tctaactgcc tctaatcctt ccaatacttg gatggacgag tcattgtatt
    11161 cgttgtttac tttttttgcc aaggcaaacg ctccttcaat aatactttgc ggcaaaaccg
    11221 caccacgatt atcatactag aaaaggtcgt gaaaaaaaag agggtagacg agaaatcaat
    11281 tttaattatc tcctaaccgc tttttcgaca ttcccctcta tcgatcatct cttctttgtg
    11341 gtcattttta tttttggttt tctttcaact taccaagttc aatcttaata caacgatcca
    11401 ttacgatatc tgtacgtcct gcttttctta gcatctcagc tgcttctttg ttttcaatcc
    11461 ctaattgtgc ccagaacact ttagcatcag ttttcaaaaa atcacctgct atttcaggta
    11521 aaaattcact ccgtctaaat acatctacga tatcgattgg atcatcgatt tctgtcaaat
    11581 tcgcatagac tttctcgcct agaatttctt caccagccaa taatggattt actggtatga
    11641 ttttatatcc attgtcttgc aatacctgtg caaccatgta acttgttcgt tctggtttat
    11701 tgctcaagcc tacgacggca atattttttg cttcttccaa atagttgaaa atcgtttctt
    11761 gtgttggatt ttttacagac atttctgcca ccccttgctt ttctttttat tccatctatt
    11821 atacttgtat ctttcatttt ttcaattgtt cctgttacct attcatgcta aaataaagga
    11881 tagttttcaa cttgaaagag ggtaaaaaat gaaattgatc ttattactta ttttagcata
    11941 tcttttaggt tctattcctt ccggtgtctg gattggcaaa ttgtttttca aaaaagatat
    12001 ccgacaacac gggagcggta atactggaac gaccaataca tttcgtgtat taggcaaaac
    12061 agccgggatt gttgtcctgc ttatggatat tttaaaaggt acattggcta cttgtttgcc
    12121 gatgatcttc cacctttctg ggattaaccc tctttggttt ggggtatgcg ccattcttgg
    12181 ccatacattt ccgatttttg ctgggttcaa aggaggaaaa gctgttgcga caagtgcagg
    12241 aatgctttta ggatttaatc ctgtattttt tgtttattct tgtatcatct ttatcgttag
    12301 tttattttgt acaagtatgg tgagtttgag cagtatgatc agtgcagtat tgatcacttt
    12361 atctacgatc gtccttcctt atgtagctcc tgtgattttg gctaagccca attggttact
    12421 gacgatcatt gcctttttag tttcttcctt tatcttttat cggcataaag acaatatcaa
    12481 gcgtatcaga aaccatactg aaagtcgaat cccgtttggt ttgaatagca gcaagaaaaa
    12541 atcctgatac tttttcattc caaagagata aaacgggagt gtgacaaatc gatgtcacac
    12601 tcccttatta cttacaggat agctttttta cacaccttgg taaacggtgt tccctgctaa
    12661 cttctcggtc acaacctctt acttcactgt gatggaatat tttgtcttga agatttcatg
    12721 ggcacctagt tgattgatcc ctactttttc catcaagttt ccactactgt tcaccgtgtc
    12781 agcgattccg caccacggtt cgatacaaac gaaaggcgct tcttttggat aaggagacca
    12841 gatccctaca taaggcatat ctttatagct taaagttacg ctatgggaag atttttcact
    12901 tcgaatggcg tacgcattta gaccccttgt ttcaaaaacc aaggcatctt gctcaaaaag
    12961 ttcacgattc aaagccagat tcgtatttgt ctgacctaac gtcttttgat ccatatcgat
    13021 gaatgggcct ttcaatggta ggcgtaatct tgatttcaat ggagaaaatg ccagatagaa
    13081 atcttcaaat gacaatcctt cttcaagagg tacattgaaa gcaggatgtc caccgataga
    13141 aaagaacatt tctttttcac cggtatttgc cacttgataa tgaaccgtga tgccgtctcc
    13201 tcctaattca tagctgatca ctaattcaaa atcaaatgga tacttttcta atgtctccgg
    13261 cgtactcctt aagatgaaac tagccatttc ttgtccatgt tcaatgactt caaaatccat
    13321 atcccgagca aatccgtgtt gccccatttg atatgttttc ccttgataaa catactgatc
    13381 atcttttaaa cgtccaacaa aagggaataa aaccggtgca tgtctgcccc aatacgctgg
    13441 atcaccttgc cagatatact ctaaaccatg ttcttttgat ttcagactga tcaattccgc
    13501 accgtgttca gcgatttcgg cgatcagctg gtcattttga ataataacag tcattgccat
    13561 tcctccaact tcgacttact ttttaaaatg ggaaggctgg aacaaacatt tctatctgcc
    13621 ccagtcccct tacttctaat aactgtgttt tgctaactcg tctttaaaac gggaattcaa
    13681 tacttttaaa taagttccct tcatgcctaa cgaacgagat tcaatgattc cagctgattc
    13741 caattttcgt aaagcattga cgatcacaga acgagtgatg ccgatactat cggcaatatt
    13801 agaagcagtc agacgacctt catctccatc caatgcttca aagatagctt gtacagcctt
    13861 caattcgcta taagataatg tgttgattgc catttggaca gccgttgcac tccgtacatc
    13921 tgcttcgatg cttcgagact tctgataaag gatctgcata ccgacaaccg tagctccata
    13981 ttctgctaag actagatcgt catctgcaaa cgattgttcc atacgagata agataatcgt
    14041 acccaaacgt tcgccagctc caaagatcgg aacgacagtc gtcagaccaa atggatattt
    14101 ctctcttgac tctaccggaa atgcagtaat atcactagta attgggatat tagcttcagt
    14161 tttcatcaac tgtgccactg tatcagtata agattcagga aattgtttct caacaaacat
    14221 tgttttgatt cgttcattgt tcacatcgtg acgttcatta taacctagta atactccgtc
    14281 acttcctatg atataggtgt tgctgtcaag aataccacct agtgtgacag ccattttatc
    14341 gtaaggcaaa tctgcttgca tgtcaaaagt attcttttgc tgcaaaagtg tgtttatctt
    14401 gcgggttttc gttagcaagt ctgtcattgt cgtcctccga ttttttataa aatatagcga
    14461 cttaaatctt catttgccgc aattgagcca agtttgtcat ccacatatgc ttctgtgatc
    14521 gttatttctc ccatttgcat atctggtgct tcaaataata gatcttctag tagtttttcc
    14581 aaaatggtat gcagtctgcg tgcgccgata ttatctgtgt cacggttgac atcgaaagcg
    14641 atttgagcga tccgttcaat tgcttctttt gtaaacgtaa ctgacacatt ttctgtaccg
    14701 atcaacgcga cgtattgttt tactaaagca ttatttggtt cggtcagaat cttcacaaaa
    14761 tcttcagctg tcaaatcatc taattctacc cgtatcggga agcgtccttg aagttctgga
    14821 attagatcac ttggtttaga caaatggaaa gctcctgaag caatgaatag gacgtgatct
    14881 gtttggatga tcccgtattt tgtgtttact tgtgaacctt cgacaattgg caatatgtct
    14941 ctttggacgc cttctctgga tacttctcca gattgctgtg atttagaagt gatcttatcg
    15001 atttcatcga taaaaataat tccactttct tccgctaatt tgattgcctc actatggata
    15061 tcagcatcct tgactaattt tgctgattct tccttgatca ataactctaa agcttctttg
    15121 actgttaccg tccgttccac ttttttcttc ggtgtcaaag cacctagagt ttcatttagg
    15181 tcgatcccca tttgctccaa tccattgttc atcattggtg caggtttctt ttggtcttca
    15241 acttcaatgg ttacttcacg actatccagt aaaccctttt gtagttgttc caagactgta
    15301 cggcgattga ttcgaatatc ttctgtcact tcttcttgcg gttcagcggt attttgctga
    15361 ctagcattaa aaagattcat catttgttca aattgattag tggattgttt ctgttctttt
    15421 ttgataccag gtaccaagat ttttaccaaa cgtttttctg catttttttt agcacttgtg
    15481 tatacacggc tgtactgctc tttttctacg atttggatcg cattttctac taaatcccga
    15541 accattgatt ctacatctct tccgacataa ccgacttcag taaattttgt tgcttcgact
    15601 ttgacaaatg gtgcattgac gatttttgcc aaacgtcgtg cgatttctgt tttaccgacc
    15661 cctgtaggtc cgatcatcag catatttttt ggtgtaatat cttgttgcat tttttcatct
    15721 aactgcaaac ggcgatagcg gttacgcaaa gcaacggcaa cagatttttt tgctgcgtgc
    15781 tgtcctacga tatattcatc taattcactt acgatttgtc ttggtgtttg ttttaattca
    15841 tacatatcta ctcacctctt acagttcttc tacgataata ttatgattag taaagataca
    15901 gatatctgcg gcaatattta acgcattttc tgcaatttct ttcgcactca tgtcgttttg
    15961 agcatagttt ttcattgctc gggctgcagc caacgcaaaa ttccctcctg atccgatagc
    16021 taagatacca tcatctggtg tgataacttc tcctgtacct gaaacaagaa gcatttcttt
    16081 atcattcatt acgatcagca tcgcttcaag tttttgcata gattgctgtg tccgccattc
    16141 ttgagctaat tcgacagctg cgcgttgtag attcccgtta tattcgttta gtttgccttc
    16201 aaatttttct tctaaagtaa acgcatctgc aacacttccg gcgaaaccta caacaacttc
    16261 tccattgtag atacgtcgta ctttctttgc agtgcctttc atgacaacag attcacccat
    16321 tgttacttgg ccatctccag ccatagcaaa tttcccgtct ttttcgacgg cacagatagt
    16381 ggttgaatga aatggtgatt cgaccatgtt tgtttcctcc taaatttgtt cttttcctta
    16441 tttttacgct cttggatgga aagaacgata gtttttttgt aaactttctt ttgttacatg
    16501 ggcatagatc tgggtagtcg ataaattggc atgtcctagc aattcttgga ctgtccgcaa
    16561 atctgcccca ttatttaata aatgagtagc aaatgtatgg cgcagcatgt gaggatggat
    16621 atcactatcc aaactgcttt ttttgatcat ttgattcaag acatattcaa ttccagtagg
    16681 cgtgattggt tttccatgat gattcacaaa aacgatatca tgctcttcgt gatattttgt
    16741 catcaatacc tggcggcctt ccctgaaata ttcttgcaga gcatcttgag caaaagaacc
    16801 tagaggagca tatctctctt tgttcccttt accatggatc aacatgacct ctgaatcaaa
    16861 atcaatgtct gataccgaca aactgctaca ctcacttaaa cggatgcctg acccgtaaag
    16921 gacttccagt aaagcgcggt tcctcaaatc caaaggacta tctcctgcta cgctgtcaaa
    16981 aagtgcttgc atttcatttt cataaaaaaa acgtggcagt ctcaaatttt tctttttgag
    17041 atgaacgtaa gaaaatggat tttcttttat cacttcttct ttgagaagat actgataaaa
    17101 cgaacgtagg ctggctatct tgcgactgat agaatttctg ctatagctag gacgatgatc
    17161 agctaatgca ctcaaataga cacgaaccgt aaaatattcc gccttcagca gatcagcgtc
    17221 tccagattcc tccaaaaaat gtttgaaatg gagaatgtct tcttcatatg cttctttcgt
    17281 tttttctgaa tatcctcttt cgacaatcaa ataacgcaaa aatttttgtt cccacgtggc
    17341 ttctgtcatt ttctcacctt ccttttgtcc cagagaataa tttagcacag cgtttataaa
    17401 aagacaaata aattgtcaga atttaaccaa tatttctaag tttgttcaca attggcgcaa
    17461 ttcctcacat tttgtttgag aaattcttaa gaaaaacgca agaaaaagag cgagactatc
    17521 cgtctcaccc tcttcatgga gtttccttac tcatttggga ttgcagtttt ttctaattct
    17581 gtttttattg cctcattagc ttttaaagct cgttgtgcaa ttgcttcata tcttgctttt
    17641 ttgtctcgaa tgcgctctgg cagttcagga aacagaccat agttggcatt catcggttgg
    17701 aaatgttttc cttccgcatg agtaatgtag taagccatac ttccaatagc tgtttcttgt
    17761 ggaaactcga ctggttccat ttcttttgct aagcgtgcgg cgttgattcc agcaagtagt
    17821 ccgctcgctg cactttcaac atagccttct acaccagtca tctgacctgc aaagaacaga
    17881 tcttcgcgtt tcgttgattg gtaagtcggt ttcagcaatt ctggcgaatt cataaagctg
    17941 tttcggtgca tcacaccata gcgaacgaat tcagcatttt ctaacccagg aatcatccgg
    18001 aaaacacgtt tttgctcgcc ccatttcaag tgagtctgaa agcccacgat gttgtataaa
    18061 gaagctgcag cattgtcttg acgcagctgg ataacagcat aaggacgttt gccagtttta
    18121 ggatcttcca atccaactgg tttcataggt ccgaaaagca tcgttttgaa tcctcgctgt
    18181 gccattactt caattggcat acatccttca aaatattttt ctttctcaaa agatttaagt
    18241 tctgccactt cggcattgat tagtgcctca tggaaagctt tgaattcctc ttctgtcatc
    18301 ggacagttca agtaagctgc ttctcctttg tcatagcggg acttcaaata tactttatcc
    18361 atatcgatcg tagctttgtc tacgattggt gcagctgcat catagaaata aaaaccatct
    18421 gaaccattga atgctttgat gctttctgct aatggatgag aagtcaaagg tcctgttgct
    18481 actacggtga ttccttccgg tatttcagta atttcttcgt ttatcactgt tactaatgga
    18541 tgctctttga cttttcgggt aattgtttca gaaaaagaat cgcggtcaac agctaaggca
    18601 ccaccagctg gcactgctgt ttcatctgcc gaagtgatta ctaccgaatt caatcggcgc
    18661 atttcttctt tcaataaccc tactgcatta gccaaactgt ttcctcttaa tgaattggaa
    18721 catactagtt cagcaaaatt acttgtgtga tgcgcctcag tcgatttgac tggacgcatt
    18781 tcatataaac ggacagggac tcctgcttgt gcgatctgcc aagcagcttc acttccggca
    18841 agccctgctc caacaacatt taccattttc aatgaaagaa cctcttttct attatttttg
    18901 gatgtcttct tcgtaatctc catttacaca gactacttgt ttaccgcctt tgactttctt
    18961 ctcgaccaga tatccgtcac attttggaca atttctgccc actggtttat cccaagaagt
    19021 gaattcacat tctgggaaac gggaacaacc atagaatagt ctgtttttct tagattttcg
    19081 ttcaatgact tgtccttcat gacatactgg gcaagtcaca ccgatttctt ttacgattgg
    19141 tttcgtattc cgacaatcag gaaaattact gcaagcataa aacttcccat atttccctaa
    19201 ttttatcacc atcggatgtc cacagacatc acaatcgaat cctgctggtt catctttgat
    19261 ttgaattttt tcgatttttt cttccgcatt cgtcagttct ttttcaaacg gtttatagaa
    19321 acgatcaacc actttgaccc acgcttccgt gccttcttca attttatcca aatctccttc
    19381 catttctgct gtgaaatgga tatcgacgat ttgagggaag aattcacaga ttaatgaatt
    19441 gacaatttct cctagttcag tcggctcaaa tcttttttgt gtcaatttta catagtagcg
    19501 tctttggatc gtatcaagtg ttggtgcata agtggaaggt cgccctaccc cgttttcttc
    19561 tagtgcacga atcaacgtag cctcactaaa acgtgctggt ggttgagtaa aatgttgttt
    19621 tggttcaata tcaacagatt ggactacatc gccttcttca agttctggaa gaatattttc
    19681 tttctcttct ttgccgtcat ctctaccttc cacatacact tgcatgaatc ctttgaattt
    19741 gatttttgaa ccgttcgcaa tgaagatgac accattttgt tctaatgtca ctttcattgt
    19801 atctaaaata gctggtgtca tctgactagc cacaaaacgt gaccagatca atgtataaag
    19861 tttcaattga tctttatcta aatattgttt gagttcatct ggtgtacgaa gcacactcgt
    19921 tggacgcaca gcttcatggg catcttgtgc gccttgagca tttttgatct ttcggtgatc
    19981 atgtctagaa aattcttttc catacgtatc ttcaataaat gctgcggcct ctgcttttgc
    20041 tgtatcggca atccgggtag aatcagtacg catgtatgta atcaagccga ctgttccttg
    20101 tttacccaaa gtgattcctt catataattg ctgtgctacc atcatggttt ttcttgttcg
    20161 aaagtttaac ttacgggcag cttcttgttg caggctactt gtagtgaaag gattcgcagg
    20221 gtttcttttg cgctctttct tttctacttt tttaacttcg aagtctgttc catcaattct
    20281 agcagtgact tcttttacac tttgtgcatc tggtaatttc tttttcttac cgtcgattcc
    20341 ccagaaattt gctttgaatt tcttgcgagc ttttttaaaa ttaccgtcga tgctccaata
    20401 ttcttctgga acaaattctc ggatctcttt ttcacggtca ataataattt ttaaagcgac
    20461 agattggaca cgtcctgcac ttaacccttt cttgacctta cgccagagaa taggactgat
    20521 cgaataacca acgattctgt ctaaggtacg acgagcttgc tgtgcatcta ctaaatctac
    20581 atcgatcgtt cttggttcct taaaagctgc tttgactgcg tcttttgtga tttcattgaa
    20641 gaccacccga tttttttctt tcagatccaa cccaagtagg tacgctaaat gccaagcaat
    20701 cgcttctcct tctctatccg ggtcacttgc caagtaaact ttttgagctt tttttgctgc
    20761 ggctttcagg cttttgatga catcgccttt tccgcgtata gaaatataat ggggttcata
    20821 attgttttca aaatcgatac ccattttact tttcggtaaa tctcttatat ggccaacact
    20881 ggccattact ttatagtttc gtccaagata tttctcaatt gtcttggctt ttgcaggtga
    20941 ttctacaatc actaaatatt tatacgccat tactgaatgg actcctttcc attgtcgtgt
    21001 tcctactatt aaaagaggat tcatacaaat cgtagctaat catatccatt cagaaaacgt
    21061 ttgtcaagaa aagcaaatga aaagtatggt gttattcttt aaattctgcc aagatatctt
    21121 cacccgaaac cgtgcaaact gcaccgtctt ggatcaaatg atgacatccg gtagaatgtt
    21181 ctgttaaaat atttcctgga acagaaaaaa ctgttcttcc aagatccaat gcttgctgtg
    21241 ctgttatcag agagccgctt tttttcttgg cttcaaagac acatgttcct ttagacaaag
    21301 ctgcgataat ccgatttctc attggaaaac aatgcttttt cgggccggat tgaggaggat
    21361 attcactcaa aagaagatga tcatttgcaa tcacctcttg caagtcacga ttttcagcag
    21421 gatagacttg ctctatccct gttccaatga ctgcgattgt ccgtccacca tgagagattg
    21481 ctgcttggtg accgtatgta tctatccctt tcgcgcagcc actgacgatc acatatccag
    21541 cttctatcac tggttttagt aaataataaa ctgttttttt ggctaaagca gaagtgtgtc
    21601 ttccaccaac aacgcttaac atgttacaag aaagtaaaga tagatcacca ttataaaaca
    21661 gtacaattgg cggataggtc aagtgaagca gctgaggggg atatttggga tcaaagcaag
    21721 tgataaacga ctgtttcgat tggattttgc cccagttagc gtgaatttcc tgccaacttt
    21781 tgcgaaacag ctcccggtgt ttggttattt tacctatctt aatgatgtct tcaaatccga
    21841 tttctttata aggatactgc atcatccagt tagcaagtgc ccattttcct tgactactga
    21901 ttccttttgt ataagcaagt ttcaataaat tttcttctat ttgatacatc ataaaaccgc
    21961 ctttcgtctc tctttctaat aagatacgta aaagcggctg ttttttattg taaatatttt
    22021 tttacaggtg caaaactttt acgatggatt ggcgtaattc cttgtcgttc aagtccttct
    22081 aagtgttcct ttgttccata acctgcatta tgcgagaaac catatccagg ataaagcacg
    22141 tcatattctt tcatcaattg gtctcgatat actttagcaa tgatacttgc agcagcaatc
    22201 gacacagatc gtgcatcccc tttgattatt ttttcctgct gaataggcaa atccaaggtc
    22261 atcgcatcga tcaacaaata gcttggtaca atggacaatt tttctactgc ttggatcatc
    22321 gctaatttac ttgcttcata aatattcaca tcatcgattg tctgctgatc aacgatcccg
    22381 atcccgattg ccagggcttt ttcttgaact tgttgataaa gaagttctcg ctttttctct
    22441 gataattgtt tggagtcatt cagccctaat atgatctcat ttttcggcaa gataactgca
    22501 gctgccacga caggtccagc taatggtcct cttcctactt catcaatgcc ggcaatccat
    22561 tggtgacctt gtgcgtaccc ttgcttttca aagcaaaaca tttcttcata tttttcctta
    22621 attaatgctt cctttttcaa tcgtcgttca aaagaagcca gtgcttgctg cacgcctttg
    22681 cgtggatcat ttcttaatga ttctagtgta ggatcatcta ttctctgtat gcttttcaat
    22741 cgttctttga tactttggat ggattcagtc atcacttttt tgctccattt cttcgcaacg
    22801 atccagtgtg tatcgtccaa gttttccttg acgaatatca tgcacgacta attcgctgcc
    22861 acgatcatag tcttctttat acccacgttt tgctgtaata tccatcagta attccggtgc
    22921 caatttttct tcttgctcga ttgataaacc ataacgatcg ctgattcttc ctgaataata
    22981 tcgggcaaaa aagtctaatc cgtaaatcgc taaatcatct aaatggacaa gctggtcttt
    23041 gatcgcgcct gtcagcgcta gttttttgcc gatttcttca tcttcaaatt tgggccataa
    23101 aatccctggt gtgtctaaca attccaactc attacctaaa cgaagccact gctgaccttt
    23161 tgttacgcca ggcttatttc ctgtctgtgc aattttcttg cctactagtc gattcatcaa
    23221 cgtagatttc ccaacatttg gaatacctaa gaccattgcc cgaatagcgc gaggcttgat
    23281 cccccgagca gcatctcttg cccgtttttc tttcaatgct tctttagccg caggtactag
    23341 acctttaatt cctttacttt cttgtgccgt gatagctaat gcaagatggc cttgtttacg
    23401 aaaatattct tgccacagct ttgtctgttg tggatctgcc agatccgctt tattcaataa
    23461 aacaaggcga ggtttttgct gaatgatctg atctagcatt ggattacgag aagataaagg
    23521 taatcgtgcg tctaccaatt caaatacgat gtccacatat ttcatttttt cagaaacttc
    23581 tcgtcgggct tttgctatat gtcccggaaa ccattggatg gtcattgttc tctccattcc
    23641 tttcataccg gaatattccc cggctttact tgtttttttc tttttcaata taaattcact
    23701 tttttattat aaagaaattc catctattag ttaagtgaaa gtgaaccagt cctcattcta
    23761 tcacatcttc acttttttga tgatgcaaat ttttattttg gtttctatat atgaatctaa
    23821 atttttgaca gcgtttccta aataagagat gatagacgaa caaaacagaa aggagacgaa
    23881 acaaatgaat agtatggttt ttgttaattt tcctgttgct gatgtcaatc gttccgttgc
    23941 tttttatgaa aagctgggtt ttaaaaaaaa tgaagaattt tctactgaag aagctggttc
    24001 aatggtatgg gatgacactt tttggataat gcttctctct catgattttt ataaaaaatt
    24061 tataaaagat aaagaaatag ctgataataa aaaaacaagt ggtgctttga tttcgtttag
    24121 tgttgaaaat gcggatgaag taaaaaaatt tgctcaaatt gcaaaagaaa atggtggtac
    24181 tttccacgaa gtagatatgg gaatgcctga agaagaaatg ttttcattag aagtccaaga
    24241 tctagacgga aatacattag aacccgtatg gatgaagatg taagcagata actaatctga
    24301 cgttttttat aacaaaaaaa caaaggcgga tgatacgata agtattctcc gcctctattt
    24361 tttttattcg tacaatctct tttttacagt ttataaaagc agtcttctca cccttttttt
    24421 aattgttaga tgccagtgta tgtaaatcac caatgtgtca tgacacattg gtgattttat
    24481 ggtatccttt tattattaac tgacaaagga gagaatattt tgaacacttt gattgcaaaa
    24541 gacgttttta gattgcccga aatgaatgca agattgttgt cgacctctgc tcttttatta
    24601 gcccttgatg tcatcttata caagatagcg attggtcctg cttattttca aatcacattc
    24661 ggatttttaa gtatggcgct tatgggttat ctatatggac cgatctgggc tggtttgctt
    24721 gaaatgttgt cgaacacttt aaatttcacg atatttggtt caggaacgtt tcaagctgct
    24781 tttcttatca cggctttcct tggtggattt attaatggct tatttttata tcataaaaag
    24841 ataaacctgc cttccgtcat cgctgcccaa ttattgatta tgatcccagt cagccttttt
    24901 atcaattctt ggctgatgtc tgttttgttt ggtgcagatt tcaaagtaat ttttctaggt
    24961 agattcatca ggaatatgat ccaaatccct attcaaataa tggttgttta ttcatttatc
    25021 aaaactttag aggctagaaa agtgtttaaa cgagctgttt gattttacgt actaaggtag
    25081 taaactgatt ttggattaaa caataaagcg tgcagtctca acagactgca cgctttattg
    25141 tttctcactg gatccatttt atttcatcaa gtggataata gacaaatctt gctttaccta
    25201 gaatatcagt tttattgatc gtgccaaacg aacgactatc ttttgaaact cttcgattat
    25261 ctccaagtac aaaataatta tcttccggaa gcttggattg tcccgttagc tcttgtagtg
    25321 taaagtccgt cgtataggat gcatttgcat gatctgattt caggttttta gttagataag
    25381 gttccttaac tactttccca ttgatcttca attgatcatt ttggtagctg acattttctc
    25441 ctggaagacc gattactcgt ttgatataaa tcgtgccatc tgctaattga aaaacaacaa
    25501 tatcaaatcg tctgatctcc gaaaattttt ccatcacaac catatcacct tgcttcaaca
    25561 cgttttccat tgaattgcct tccactggta ccgggatgaa taaaaatccc cgcaacatga
    25621 acgcaaggaa tacagaaaca agtaagtatt tgaatacaag ccagaagcga ttaatatagc
    25681 gttgtttttt tgtcaatctc tatcaatcca tccctctttt tttcattctt tctttattat
    25741 aagaaaaata tcaaaaaaat gcgactagat agctgaaata aaaataattc tttcaattag
    25801 gctattggac aaaaaacttt tttgatcata aa
//
LOCUS       DAZAJZ010000037        25471 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000037 DAZAJZ010000000
VERSION     DAZAJZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.037"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..535)
                     /locus_tag="ACG86D_001429"
     CDS             complement(170..535)
                     /locus_tag="ACG86D_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140578.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            743..1225
                     /locus_tag="ACG86D_001430"
     CDS             743..1225
                     /locus_tag="ACG86D_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140579.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1422..2312
                     /locus_tag="ACG86D_001431"
     CDS             1422..2312
                     /locus_tag="ACG86D_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFN3140580.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2638..5112
                     /locus_tag="ACG86D_001432"
     CDS             2638..5112
                     /locus_tag="ACG86D_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3140581.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5215..6951)
                     /locus_tag="ACG86D_001433"
     CDS             complement(5215..6951)
                     /locus_tag="ACG86D_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3140582.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6948..7652)
                     /locus_tag="ACG86D_001434"
     CDS             complement(6948..7652)
                     /locus_tag="ACG86D_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3140583.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7783..8286
                     /locus_tag="ACG86D_001435"
     CDS             7783..8286
                     /locus_tag="ACG86D_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN3140584.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8246..8584
                     /locus_tag="ACG86D_001436"
     CDS             8246..8584
                     /locus_tag="ACG86D_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN3140585.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8605..10023
                     /gene="ffh"
                     /locus_tag="ACG86D_001437"
     CDS             8605..10023
                     /gene="ffh"
                     /locus_tag="ACG86D_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN3140586.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10135..10713
                     /locus_tag="ACG86D_001438"
     CDS             10135..10713
                     /locus_tag="ACG86D_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN3140587.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10717..11238
                     /locus_tag="ACG86D_001439"
     CDS             10717..11238
                     /locus_tag="ACG86D_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN3140588.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11317..11871)
                     /locus_tag="ACG86D_001440"
     CDS             complement(11317..11871)
                     /locus_tag="ACG86D_001440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN3140589.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12124..12399
                     /gene="rpsP"
                     /locus_tag="ACG86D_001441"
     CDS             12124..12399
                     /gene="rpsP"
                     /locus_tag="ACG86D_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN3140590.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12411..12662
                     /locus_tag="ACG86D_001442"
     CDS             12411..12662
                     /locus_tag="ACG86D_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN3140591.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12786..13577
                     /locus_tag="ACG86D_001443"
     CDS             12786..13577
                     /locus_tag="ACG86D_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140592.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13747..14268
                     /gene="rimM"
                     /locus_tag="ACG86D_001444"
     CDS             13747..14268
                     /gene="rimM"
                     /locus_tag="ACG86D_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN3140593.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14258..15019
                     /gene="trmD"
                     /locus_tag="ACG86D_001445"
     CDS             14258..15019
                     /gene="trmD"
                     /locus_tag="ACG86D_001445"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN3140594.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15155..15502
                     /gene="rplS"
                     /locus_tag="ACG86D_001446"
     CDS             15155..15502
                     /gene="rplS"
                     /locus_tag="ACG86D_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN3140595.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15812..16054
                     /locus_tag="ACG86D_001447"
     CDS             15812..16054
                     /locus_tag="ACG86D_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN3140596.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16142..16288)
                     /locus_tag="ACG86D_001448"
                     /pseudo
     CDS             complement(16142..16288)
                     /locus_tag="ACG86D_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16345..16659
                     /locus_tag="ACG86D_001449"
                     /pseudo
     CDS             16345..16659
                     /locus_tag="ACG86D_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16688..17947
                     /locus_tag="ACG86D_001450"
     CDS             16688..17947
                     /locus_tag="ACG86D_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140597.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18000..18896
                     /locus_tag="ACG86D_001451"
     CDS             18000..18896
                     /locus_tag="ACG86D_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140598.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18907..19776
                     /locus_tag="ACG86D_001452"
     CDS             18907..19776
                     /locus_tag="ACG86D_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140599.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIELFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19786..20461
                     /locus_tag="ACG86D_001453"
                     /pseudo
     CDS             19786..20461
                     /locus_tag="ACG86D_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            20667..22331
                     /gene="alsS"
                     /locus_tag="ACG86D_001454"
     CDS             20667..22331
                     /gene="alsS"
                     /locus_tag="ACG86D_001454"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN3140600.1"
                     /translation="MAEKMKQGSKSVVERLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22344..23054
                     /gene="budA"
                     /locus_tag="ACG86D_001455"
     CDS             22344..23054
                     /gene="budA"
                     /locus_tag="ACG86D_001455"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN3140601.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23184..23681
                     /locus_tag="ACG86D_001456"
     CDS             23184..23681
                     /locus_tag="ACG86D_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN3140602.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            23866..24126
                     /locus_tag="ACG86D_001457"
     CDS             23866..24126
                     /locus_tag="ACG86D_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140603.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(24488..24727)
                     /locus_tag="ACG86D_001458"
     CDS             complement(24488..24727)
                     /locus_tag="ACG86D_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140604.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25013..25327
                     /locus_tag="ACG86D_001459"
     CDS             25013..25327
                     /locus_tag="ACG86D_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140605.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8455 a         4307 c         5237 g         7472 t
ORIGIN      
        1 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
       61 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
      121 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      181 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      241 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      301 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      361 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      421 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      481 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      541 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
      601 atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc attatctcta
      661 aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa
      721 aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt
      781 tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg
      841 tgcggagata ttaggcctga tccaaggaag cggattagtg gtttctagga attttccagc
      901 tttgactgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa
      961 tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc
     1021 tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattc
     1081 agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc
     1141 atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc
     1201 tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaagcat atgagctgcg
     1261 taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg aaaaacgttt
     1321 acatgatttt tacataactt aactatattt ttacagcaac catacgaggg atttacagtc
     1381 gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa gtattgttta
     1441 ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga aatacagatg
     1501 gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc gttgcggtag
     1561 gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta caagaaaatc
     1621 caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc caagtcgaag
     1681 ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac ggagttgatg
     1741 cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc gtcaataaag
     1801 atacagatgt caaagatatc accaaacagg aattgattga tatctttaca ggaaaaatca
     1861 ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg
     1921 gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt
     1981 cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa
     2041 tcagctattt agctttctca tatctcgatg attccacaca agcactaagt atcgacggtg
     2101 tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca tatgaacata
     2161 tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac atgatgacag
     2221 aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca atgatggaag
     2281 tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc acggtagtcg
     2341 ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct ttaattgtga
     2401 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2461 accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtagcttttt ttttgctata
     2521 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
     2581 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
     2641 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
     2701 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
     2761 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
     2821 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     2881 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     2941 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     3001 acagcaaaag tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt
     3061 gtaatagatg acattgttat tttagagact ggacatcaaa ttccagcaga tgcagaggtc
     3121 gtggaaggga aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     3181 aaagaaatag gcgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     3241 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     3301 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     3361 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     3421 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     3481 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     3541 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     3601 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt gatcgtctct
     3661 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     3721 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     3781 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     3841 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     3901 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     3961 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     4021 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     4081 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     4141 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     4201 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     4261 aagaaaatct tcgttcaatt gctaaagcgt cgtaacaata cagtagctat gacaggtgat
     4321 ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga
     4381 aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg
     4441 ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg
     4501 tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg
     4561 acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca
     4621 tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat
     4681 gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg
     4741 atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt
     4801 ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga
     4861 agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag
     4921 ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc
     4981 tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa
     5041 aatgaaaaag agcaaaagcc gtttagtcgc gtagttaaaa aatttctatc gatatttaaa
     5101 cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc
     5161 tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat
     5221 tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt
     5281 accatgaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga
     5341 atgccggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc
     5401 aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata
     5461 gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt
     5521 gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg
     5581 aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata
     5641 aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat
     5701 gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga
     5761 agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag
     5821 tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt
     5881 ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc
     5941 tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat
     6001 ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg
     6061 ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag
     6121 ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc
     6181 aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtat acgcccgata
     6241 catcttactc atttgttcgc tcaacgcatt gatcgtctga tacaattctt cccattgatc
     6301 gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg
     6361 cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag
     6421 aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg
     6481 taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt
     6541 gactggtaat gcgacatata aaagatcccc attcaaagta gtacttttgc gcaaagcgct
     6601 acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt
     6661 caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg
     6721 ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt
     6781 ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg
     6841 ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact
     6901 aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt
     6961 tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct
     7021 cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg
     7081 aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt
     7141 ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct
     7201 acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc
     7261 tcagcttcat cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg
     7321 gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg
     7381 ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt
     7441 ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct
     7501 aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg
     7561 gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa
     7621 ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc
     7681 tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta
     7741 taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca
     7801 tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg
     7861 gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca
     7921 attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa
     7981 gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc
     8041 aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc
     8101 ccttcatgcg gaacccattc gatttatgac gggagttttt ccggaataaa aaaaaatggt
     8161 gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa
     8221 tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct
     8281 ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta
     8341 tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt
     8401 atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa aacttcatct
     8461 tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga
     8521 taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga
     8581 ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag
     8641 gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg
     8701 cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt
     8761 actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca
     8821 caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca
     8881 gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca
     8941 ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt
     9001 ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta
     9061 ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc
     9121 gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg
     9181 gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg
     9241 acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac
     9301 gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat
     9361 ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa
     9421 ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct
     9481 agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactat
     9541 gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat
     9601 gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa
     9661 atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat
     9721 gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat
     9781 ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa
     9841 gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa
     9901 ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta
     9961 ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa
    10021 taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat
    10081 cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga
    10141 tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt
    10201 cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga
    10261 ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc
    10321 gatattaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa
    10381 ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg
    10441 aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa
    10501 atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat
    10561 gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat
    10621 gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa
    10681 gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt
    10741 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
    10801 actccaacag gtaagcaaac cacttatgaa gctttttatg aaacaatcga tacgaccatc
    10861 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
    10921 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
    10981 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
    11041 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
    11101 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
    11161 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
    11221 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
    11281 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
    11341 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
    11401 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
    11461 cacattcaaa aagacaagtg attgtcgtag atggtggttt gcgccaaagc cgccgatccc
    11521 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
    11581 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
    11641 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
    11701 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
    11761 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
    11821 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
    11881 cctccttcaa actatctata acaatcttac aaaaaataag taactggagc aaataaaaac
    11941 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
    12001 aaatcttcta tttcctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
    12061 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
    12121 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
    12181 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
    12241 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
    12301 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
    12361 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
    12421 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
    12481 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
    12541 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
    12601 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
    12661 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt attaattgaa
    12721 gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac
    12781 agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt
    12841 cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca
    12901 gtccagaaaa aagtagtacc aaaacggcta ccaccactga acaaacaatg gctacctcaa
    12961 ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc
    13021 ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag
    13081 gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga
    13141 atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa
    13201 caatccaagt tttgcagccc tttgagatcg acacaaatgg tcagcaagtg gacttaggtt
    13261 ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag
    13321 gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag
    13381 ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta
    13441 aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac
    13501 cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt
    13561 ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac
    13621 aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa
    13681 gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga
    13741 tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc
    13801 gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg
    13861 ttaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa
    13921 cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa
    13981 tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa
    14041 ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa
    14101 gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa
    14161 gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga
    14221 gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt
    14281 atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa
    14341 aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac
    14401 agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta
    14461 tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct
    14521 tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca
    14581 cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac
    14641 agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat
    14701 gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac
    14761 ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa
    14821 agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca
    14881 attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga
    14941 atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac
    15001 agcagatgaa gccaagtgac tttttctaaa atattcttta cagtaggttg ataatatgtt
    15061 agtattttat ggtgagtgca aaagcactta actattacga tattccgcta tgagaaaagc
    15121 tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac
    15181 aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca
    15241 cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa
    15301 acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg
    15361 cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta
    15421 cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg
    15481 tatcaaagaa atccgtcgtt aatcattgtt atcagactga gaaagaaccc ttggtattca
    15541 agggttcttt tttcttagca tgtaatttga acaccctgat ccagtttttt tctttaatta
    15601 ttggtagggg agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga
    15661 gcactatgat aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta
    15721 acataaatgg agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga
    15781 gcaagaaaaa atgaaagttt gagacacatt catgaatgat ggaaaagtaa aacaagtacc
    15841 aagttcaaca aaaaagaaaa acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg
    15901 agtgacttat acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga
    15961 ttatgtcgaa caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg
    16021 tcgggcatat cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa
    16081 aagttcaagt atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta
    16141 tctataatac aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg
    16201 agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa
    16261 aatcaattgg atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac
    16321 caaaacgtac ctcctaaaat tagattcaaa gaagatagca aagaggaaaa agatagttct
    16381 gcagcagcta tagcggcatg tggattgcta gaactttcca aacaactgcc tgtttcagat
    16441 cctcgtcatt tcgattacga agagatagca atcaaaatct tggaagaatt acagaaaaac
    16501 tatacaacga aacgagttaa cggatcaaac ggtctgcttc tccatgccgt ttatgataaa
    16561 aacagcttaa aaggtgtaga tgaatgtgtg atctggggcg attactttta tgtagaagga
    16621 atcactcgtc tagctaaaac atggtactgt tactggtgat gtataagaga agaggaggat
    16681 tcatataatg aagcgatgga aaaaaatgat gattggatca atagttttag gaatgtcact
    16741 cgtatttggt gcttgtggta atcaagaaga cagtagtgaa gaatcagcaa atgaaattac
    16801 cgtttcgaca tggaattatg aaacaacacc tgaattcaaa gcgctctttg aagcattcga
    16861 aaagaaaacg ggaatcaaag tgaaagcagt agatattgct tcagatgatt atgatacgaa
    16921 actcaccacg atgctttctt ctggagacac gacagatgta ttgacgatga aaaatctttt
    16981 gtcgtattca aattatgctt tacgagatca gttagtcgat caaacagaac gaattagaga
    17041 attagacact gatgctgctg aagaaacata tgaaatgtat gatatcgatg gaaaaacata
    17101 tgcattgcca tatcgaacgg acttttgggt actatattac aataagaaaa tgtttgatga
    17161 tgcaggaatc gaatatccaa aaaatctaac ttgggacgaa tatgaagatt tagcaaaaaa
    17221 acttagtaaa aatgacggac aagtttacgg agcatatcaa catatctggc gttcgacaat
    17281 ccaagctatt gcagctgctc aaaacgaggc gaatcttgtt gaaccagatt atcaatatat
    17341 gactgattat tatgatcgag cattgaggat gcaaaaagaa ggcgcgcaga tggattttgg
    17401 cacggctaaa tcaacgaaag taacctatca gtctcaattt gaagaacaaa aagcagcaat
    17461 gatgtacatg ggaacttggt atatggctgg catactcgca aacaaagaag caagtaagac
    17521 agaagtagaa tggggaatta cagctattcc tcagaagaaa aaaggagaat cggtcacgac
    17581 gtttggctca ccaacagcat ttgctgtcaa taaaaactca aagaaacaaa aagcggcgca
    17641 agaatttatt gaatttgcag ccagtgaaga aggagcaaaa gtattagcag gagtaggagt
    17701 agttccttca tatcgtacgg atgagattga tcagctttat tttaatctcg ctggaatgcc
    17761 aacggatgaa atttctaaaa aagctttttc tcctgatgaa atcaaacttg aatttccgat
    17821 tgatacgtat gggcctgcaa tcgataagat tttacaagaa gaatatgact tagttttggt
    17881 tggagatgaa acgccggaaa aaggaacggt aaatatggag aaacgtgtag ctgcggaaaa
    17941 agaataatca tgaaaggaga gaagccaaga gataaaacac tcttggtttc gtcatcgaaa
    18001 tggagaatag tataaataag aaagctacaa atcatttgcg tagaaaacga acgtggatcg
    18061 cttattcgtt tatcgccccg aatttcattg gttttttcgt ttttacatta ataccaatcc
    18121 tgttttcgct tgtactagca tttatgaaat gggattcttt ttcaacgcca gaatttgctg
    18181 gactagataa ctttcgtaaa atggccaacg atgagacatt ttggatttct ttgaaaaata
    18241 cttttttata taccgctgct gtagtgcctg gcacacttgt ttgttcgtta gggctagctt
    18301 tactactaaa tcagaaaatt agagggatga agctttttcg tacgatattt ttctttccgt
    18361 atgttacttc cttagtagca attgctgttg tttggaatat gttgtttcat ccaacagtgg
    18421 gacctattaa tatcttttta agacgattta ttgaacatcc tccaggttgg acttcaagtt
    18481 caaattgggc gctttgggca attgtaattg tcagtatttg gcgagggatg ggttattaca
    18541 tgattctata ccttgcagga ctacaaagta tccaaaaaga actatatgaa gcgtcttcgc
    18601 tagatggagc aaaccgttgg aaacaatttt tgaatgtgac attaccttcg ttgagaccaa
    18661 caaccttctt tgtaacgatc atgcttgtca tcaattgttt caaggtgttt gaccttgtgc
    18721 aagtgatgac ggatggcggc cctggtcgag cgacaaatgt gctggtttac caaatctaca
    18781 atgaagcatt caaaaaattt gattttggtt atgcttcagc tatttcactt gttttgttcg
    18841 taattgtttt gggcgttacg gttgttcaat tttcttggaa taagcgtcgt acttaggagg
    18901 tgtgatatgg aagagagtgt tcgaaatgaa agaaaaaaag caaaaattcg ttcaaaaaac
    18961 atgcccttta ttcaatggtt gggtatgatt ctgctgattg tattagcttt attgacgatc
    19021 attccttttt tatggatggt ttctgcttca ttcaaagcaa ataatgaagt gttaacgatt
    19081 ccgattcaat ggatccctaa aacttggcat ttagagaatt atctggttat ttggtctcgt
    19141 attcctttag tgacattttt taagaataca gcttttttaa gcgttgtgat cacgttgatt
    19201 cagttgttta cttctagttt tgctgtttac gggttttctg aactaaattt taaagggcgc
    19261 gatattttat ttttagcata tatcggcacg attgctgtcc cttggcagtc ttatatgatc
    19321 ccgcagttca ttatgatgag aaaagtgggt ttaacagata cgttgtggtc gttggtgcta
    19381 ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc aatattacag tagtatccca
    19441 gattctttat gtgagagtgc gcggatcgat ggcttatcag agtgggaaat ctatcgcaag
    19501 atcattttac ctcttacaaa accggcgatt gccagtttga cgatcatcac attcgttaat
    19561 acatggaacg attatatggg gccattcatt tatttgtctt ctacggaaaa taaaacaatc
    19621 cagttagggt tgaaaatgtt tattgagcta tttgatgcag agtacgcatt aatcatggct
    19681 gcctcagttg tttctgttct tcctgttgct tttatatttt tagcgatgca aaaatatttt
    19741 gtagaaggga tcgcttcatc aggaatgaag gaataggagg tgattatgaa agagcagcgt
    19801 tttgacaaaa atgtttatat ggaattcttt tattggttgt atcaatcatt ggcaagtagc
    19861 ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt gtcgatagat
    19921 ggaaggaatg tcttttcctt tattttttct ttacttttta ttggtccagg ttgcatcgtg
    19981 atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt acgtttgttc
    20041 ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt tgcttggata
    20101 gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta tggaaaatgg
    20161 ttgattccgt tgtttgtttt acttgcttgt tttacttgct tgtttatcag tttcctttag
    20221 catcaactgc tggtattttc aggtgagaaa tccagcgtcg aaaccaaatc aagttctacg
    20281 gatagcgttc tactatacgt tgaaaaaatg gtatgtgagt ttactagatt ttttactatt
    20341 gacaagtttg tttctcttct ttttcgtaaa gccccaatgg tgtattctac taggtccttc
    20401 tatcgtattt ggtttgattt acttcaacaa tcgtaaattg atgagaacta tggatctgta
    20461 gactttataa attgaaactt tcaattaaga atgacagttt tttatgtaaa aactgtcatt
    20521 ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacatgct atacttttga
    20581 atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta ataattaaaa
    20641 ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt tcgaaatctg
    20701 tagtagaaag gttgattaat catcatgttg attatgtatt cggtatacca ggtgcaaaaa
    20761 tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg actcgtcacg
    20821 aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag ccaggagtag
    20881 ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta acagcgacag
    20941 cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat ctattgaagt
    21001 tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa tacagtgcag
    21061 aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt atggctaaga
    21121 gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat gcacctgtcc
    21181 aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct gcagatgata
    21241 tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta gtaggtatgc
    21301 gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag acagcattgc
    21361 ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa gcgcatttct
    21421 ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa cgaagtgatc
    21481 tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg aatgcggaaa
    21541 aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct tttatgcagc
    21601 ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca ggttcattag
    21661 agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa gcaaaattaa
    21721 cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct ttagaagtta
    21781 ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat gtaggtagtc
    21841 attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg ttattcagta
    21901 atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca ttggtgcgtc
    21961 caaatacaca gatcgtatca gtttccggcg atgggggatt tttattctct gcccaggatt
    22021 tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat gatggacact
    22081 acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg
    22141 gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc cgagctacaa
    22201 gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga cccgtaatta
    22261 ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc ttaccagatc
    22321 aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac gggacattag
    22381 gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata ttagaaaaag
    22441 gaactctggg attgggaaca ttgcatgggc ttgatggtga agtcatcttt ttagatggcg
    22501 tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt gaacttaccc
    22561 cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca aaaactgcag
    22621 atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac ctatttttgg
    22681 cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa
    22741 aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa caaagcaatg
    22801 tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg
    22861 gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta atggattttg
    22921 aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag cattttccag
    22981 ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca gcagaaatcc
    23041 aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct gacgtatctc
    23101 gtttttttga ggagaaaacc tagcgtttac aaaaaaactg agctataatc ttctccatag
    23161 atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa
    23221 ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta gcaaatttag
    23281 caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca ggatattatt
    23341 tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct tgcacacgta
    23401 tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg
    23461 atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa tcagaaatcg
    23521 tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat tgcggagtaa
    23581 cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat cttttattag
    23641 caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta ttgaaacaaa
    23701 aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tgggcatttc tgtttttcgg
    23761 tttttttatt tttttataaa atcacaagtg ttattgattt tcataatcat attttaatgt
    23821 tattatcatt ttaaataatg aaaaaacatt aaggatggat gaaatatgaa taacgaaact
    23881 atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa agtgattgga
    23941 gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc
    24001 gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac tcacgtttat
    24061 gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca tactttagtt
    24121 tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt gaaggcactt
    24181 tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc gcctagagaa
    24241 tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact gccagaactg
    24301 tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc aatcatttca
    24361 aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta aagcatttgt
    24421 aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc tgaagaagat
    24481 gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt ttctttaccc
    24541 tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa tttttctcat
    24601 ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt atgttctttg
    24661 atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata aacagaattc
    24721 ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc tcagtattag
    24781 gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta gtaatacata
    24841 tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct gtattaacga
    24901 ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta ggtatgatat
    24961 tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc ttttggattt
    25021 ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt ggttttttga
    25081 taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt ctgaaaaaaa
    25141 gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt tactttcttc
    25201 aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat ctattattgg
    25261 aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga agtcaaaaaa
    25321 taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa tgtatagcga
    25381 atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa ttattttatg
    25441 ttaggatgaa ttaatttctc tagagtttat a
//
LOCUS       DAZAJZ010000038        25358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000038 DAZAJZ010000000
VERSION     DAZAJZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.038"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG86D_001460"
     CDS             complement(132..509)
                     /locus_tag="ACG86D_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140606.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG86D_001461"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG86D_001461"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN3140607.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG86D_001462"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG86D_001462"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN3140608.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG86D_001463"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG86D_001463"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN3140609.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG86D_001464"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG86D_001464"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN3140610.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG86D_001465"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG86D_001465"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN3140611.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG86D_001466"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG86D_001466"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN3140612.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG86D_001467"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG86D_001467"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN3140613.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG86D_001468"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG86D_001468"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN3140614.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG86D_001469"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG86D_001469"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN3140615.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG86D_001470"
     CDS             10661..11575
                     /locus_tag="ACG86D_001470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN3140616.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG86D_001471"
     CDS             complement(11708..13273)
                     /locus_tag="ACG86D_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140617.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG86D_001472"
     CDS             complement(13266..14717)
                     /locus_tag="ACG86D_001472"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3140618.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG86D_001473"
     CDS             complement(14731..15747)
                     /locus_tag="ACG86D_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140619.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG86D_001474"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG86D_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3140620.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG86D_001475"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG86D_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3140621.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG86D_001476"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG86D_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN3140622.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG86D_001477"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG86D_001477"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN3140623.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG86D_001478"
     CDS             21340..22764
                     /locus_tag="ACG86D_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN3140624.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG86D_001479"
     CDS             complement(22806..23063)
                     /locus_tag="ACG86D_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140625.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG86D_001480"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG86D_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN3140626.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG86D_001481"
     CDS             24337..25314
                     /locus_tag="ACG86D_001481"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN3140627.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZAJZ010000039        24966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000039 DAZAJZ010000000
VERSION     DAZAJZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.039"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..733)
                     /locus_tag="ACG86D_001482"
     CDS             complement(122..733)
                     /locus_tag="ACG86D_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3140628.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(856..981)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1063..1779)
                     /locus_tag="ACG86D_001483"
     CDS             complement(1063..1779)
                     /locus_tag="ACG86D_001483"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN3140629.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1785..2354)
                     /gene="scpB"
                     /locus_tag="ACG86D_001484"
     CDS             complement(1785..2354)
                     /gene="scpB"
                     /locus_tag="ACG86D_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN3140630.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2338..3108)
                     /locus_tag="ACG86D_001485"
     CDS             complement(2338..3108)
                     /locus_tag="ACG86D_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN3140631.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3105..3476)
                     /locus_tag="ACG86D_001486"
     CDS             complement(3105..3476)
                     /locus_tag="ACG86D_001486"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3140632.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3495..4382)
                     /gene="xerD"
                     /locus_tag="ACG86D_001487"
     CDS             complement(3495..4382)
                     /gene="xerD"
                     /locus_tag="ACG86D_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN3140633.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4478..4951)
                     /locus_tag="ACG86D_001488"
     CDS             complement(4478..4951)
                     /locus_tag="ACG86D_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3140634.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5056..5919)
                     /locus_tag="ACG86D_001489"
     CDS             complement(5056..5919)
                     /locus_tag="ACG86D_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN3140635.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6115..8214)
                     /locus_tag="ACG86D_001490"
     CDS             complement(6115..8214)
                     /locus_tag="ACG86D_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN3140636.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8434..9543)
                     /gene="rpoD"
                     /locus_tag="ACG86D_001491"
     CDS             complement(8434..9543)
                     /gene="rpoD"
                     /locus_tag="ACG86D_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN3140637.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9567..11408)
                     /gene="dnaG"
                     /locus_tag="ACG86D_001492"
     CDS             complement(9567..11408)
                     /gene="dnaG"
                     /locus_tag="ACG86D_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN3140638.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11590..13905)
                     /locus_tag="ACG86D_001493"
     CDS             complement(11590..13905)
                     /locus_tag="ACG86D_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3140639.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14090..14950)
                     /locus_tag="ACG86D_001494"
     CDS             complement(14090..14950)
                     /locus_tag="ACG86D_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN3140640.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(15009..15641)
                     /locus_tag="ACG86D_001495"
                     /pseudo
     CDS             complement(15009..15641)
                     /locus_tag="ACG86D_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15808..16569
                     /locus_tag="ACG86D_001496"
     CDS             15808..16569
                     /locus_tag="ACG86D_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3140641.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16595..19186
                     /gene="mprF"
                     /locus_tag="ACG86D_001497"
     CDS             16595..19186
                     /gene="mprF"
                     /locus_tag="ACG86D_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN3140642.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19374..20561)
                     /gene="tuf"
                     /locus_tag="ACG86D_001498"
     CDS             complement(19374..20561)
                     /gene="tuf"
                     /locus_tag="ACG86D_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN3140643.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20929..21285
                     /locus_tag="ACG86D_001499"
     CDS             20929..21285
                     /locus_tag="ACG86D_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3140644.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21526..22530)
                     /locus_tag="ACG86D_001500"
     CDS             complement(21526..22530)
                     /locus_tag="ACG86D_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN3140645.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22696..23547
                     /locus_tag="ACG86D_001501"
     CDS             22696..23547
                     /locus_tag="ACG86D_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3140646.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23564..23809
                     /locus_tag="ACG86D_001502"
     CDS             23564..23809
                     /locus_tag="ACG86D_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140647.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            23996..24700
                     /locus_tag="ACG86D_001503"
     CDS             23996..24700
                     /locus_tag="ACG86D_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140648.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7031 a         5174 c         4358 g         8403 t
ORIGIN      
        1 tgatttttat gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat
       61 tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata
      121 tttatttgac tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa
      181 gaagttgcgc atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga
      241 aaggaacgat cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt
      301 tcagccctaa aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa
      361 aaatagttag agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct
      421 gtttctttac actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac
      481 tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg
      541 aacgcaagaa cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata
      601 ccgagatatc actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa
      661 aatactgtaa aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac
      721 gggtgttctt catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt
      781 tctctccaaa tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa
      841 gcgtcgtcaa ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca
      901 ccgattcagc tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc
      961 accctgcccc tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct
     1021 attcaaaaaa cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag
     1081 caaactgctg acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa
     1141 agtcagatct ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag
     1201 catatttttc acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt
     1261 tgtcttagga tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat
     1321 ctttattcct tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc
     1381 aacgtaaact ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc
     1441 atttgtcagt agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg
     1501 ttcttttacc atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc
     1561 agaaataact cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg
     1621 gacttcatcc acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac
     1681 ttgtccgttt acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt
     1741 gatgccagcg tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc
     1801 attccatcaa agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt
     1861 gtatcaggca gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt
     1921 ccatataata tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg
     1981 gcagctagac gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct
     2041 cttgtgattg gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat
     2101 gtgtttgatg tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt
     2161 aatacataat ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa
     2221 gattctttta attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg
     2281 cttaattctt cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca
     2341 cttaatttcg tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt
     2401 ttctttttgt tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt
     2461 agtgacaact tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc
     2521 atttcttttt atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg
     2581 cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc
     2641 acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa
     2701 cccatcgatg ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt
     2761 cagtctttca gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc
     2821 tcgaggatct tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa
     2881 tgtcttgctt ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc
     2941 tagctcgagt gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat
     3001 cggtatatca tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg
     3061 accctcgaat acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt
     3121 ttctttgcta gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca
     3181 tccgggtact tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa
     3241 gacggattca aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca
     3301 ataatccctt gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct
     3361 tcagattcgt acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg
     3421 aaactcaata gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc
     3481 tcaccttcct atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc
     3541 ttcgtgatat gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt
     3601 actgtccgca aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta
     3661 tgtggtgtta cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag
     3721 attccttgcc gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg
     3781 tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa
     3841 tcacctaaag gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga
     3901 gaaagatgaa gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca
     3961 tacatgactt ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct
     4021 tcaatcagct tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc
     4081 ggcgtatcga tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga
     4141 tggaatcttc tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca
     4201 tgcaacttct ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt
     4261 ttctgctcct gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta
     4321 ttggcggaca atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc
     4381 ataataatcc ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt
     4441 ttccataatc tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttttt
     4501 ctttttcata gcattcctga cagattccat ggaacgttag ccgatgatct tttactaaaa
     4561 aatgataacg ttcttctact acttgctcca cttcaagtaa aagatcctct tctacttcct
     4621 cgatcgtgcc gcagttcaag catagtaaat gatgatggaa atgtttggcg ccttcttttc
     4681 tcaaatcata acgggctaat ccatcattaa aacttacttt atcgacaatt ttcaattctg
     4741 tcaagatttc aagggttcga taaacagtag caagtccaat ttccggactc ttttttttca
     4801 ctaaaaagaa gatttcttct gcagataaat gctctttctc attttccaaa agcacaagca
     4861 gagtagcttc tctttgcggt gtcagcttaa accccgcttc atgtagctgc tgttttgttt
     4921 ttttcatcgc cgaaatagca ttcatgacca tttactcaac cccaacaatt tataataatt
     4981 acaaataaaa aaagatataa cactatgtta attaaaatta ttataagcta gttaagaaaa
     5041 acatgcaagg gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt
     5101 aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc
     5161 aaaagctttc atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc
     5221 agaacgttct aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct
     5281 tggcttcaat gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat
     5341 ctgctcgcct agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa
     5401 atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc
     5461 tttgtttttc atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc
     5521 taaactgccc caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc
     5581 ttttttaggc catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt
     5641 atctggaaga ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc
     5701 aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt
     5761 ttgattttgg taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt
     5821 gcttaagcca aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat
     5881 aataatacct gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga
     5941 aaggttttct taatacattt ctttgctatc ataacataat gttatctact tgaatagctg
     6001 agccttggtt tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat actccccttt
     6061 ttcgaataaa cggtgttaaa cggcaggctc acagcctctt tcatttgcct aaaattaatt
     6121 gctcccattt cctccgaacc atccagtttt acagttgatg gttacgatgt aaaatggcaa
     6181 agcactttct ccaatgcctt tcatcaatgc agattttgtt gaagtggcat atagattata
     6241 atagccttcc ccattttcga ttctggcttt ttctaatctg taacctcctt caacgatata
     6301 tccacgttgg atacatgtag caatcacttt gctttggaca cctggtgccc acgtccatgc
     6361 tggattagat gcatcggcaa tatcacttgc tctggcagat ttgattggct gattcgctga
     6421 gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc
     6481 ttgttgagcg gcttgagctg cagctgctgc ttgagcggct tgttttgctt gttcttcagc
     6541 tgcttttttc gcttcttctg cttttttctt ttcctcttct tcttttttct ctttatcagt
     6601 gagcatctta tctacttgtt tcaattgttc attcaactga gcagatagat ctgtattttt
     6661 cactttttct actgcttctt ttgccgtctt gtattggtcg cgtgttgctg aatccactac
     6721 ttttccgtct ttaaagacta tagctgtttt ttctttagct gtttgcaatt cagcatattg
     6781 atttttagct tctgtttgtg cttgttgaat caattttcca aaatctgtgt tatccgtttc
     6841 gattgcttgg atctctttat ctgctttcaa cttgatgtca tctgctagat gatcatcgac
     6901 gatggcaggt gctatgaacc actcattcac tttttggata tctcggatct tagattggat
     6961 atcttggtaa gttgtttcta actctgaata tcctttttca ttcttaagac tggataaatc
     7021 attttttagt tttgataaat cttgatgaat catatctgca cgaataaatt gatggtgatc
     7081 atctgtataa aaggcagcaa gtttctcatg aagttcttcc atttgttgct gtctttttgc
     7141 ttctgctgct acttcggctt ctactttttg atgatggtca taataagccc agcttcctcc
     7201 accgagtaga agtactgctg ctaaaacgag ataaggtact ttttttgatt ttttcttttg
     7261 cgctttatct ggatctgttt tttctaagtt tgattcttga tgtgtttttt cagtatctgg
     7321 cacgattgtt attgatttcg cttctgcttc aaacagctta ggctggttct cttctttttc
     7381 tatttttgct tcaagatcag taccctcttt gcttacagat tgattttcag catcggattc
     7441 actatttatt tcatcgggct ctgttgactt tttgtctgtg ttttcgagta tttctttttt
     7501 ctctgcttcc ggcagtttat tcgttttttc cttttctaat tgtttttcag taaatacttc
     7561 agttttttct gtgatatccg tttttacgtc ttttattttt actgtttctt tacctgtggc
     7621 gttttctcta tcagagtctt tttcagtttc ttgttccttt tctggctcta ctttgttttc
     7681 ctgtgcaatt ttttcactga tcattttgag cagctctttc gcgtgctctt cttttgtatc
     7741 ttcacgatgt gctttgatat aagcggatag tatcgaattt tcttcctcat tatcgacgat
     7801 ttctttcagt tcagctactc ttcttttttg ttttgattca agcgtttctt cttgattctt
     7861 tcctgattct tccgaagtct catgaacagt tgctttcttc tgttcatttt tctctatatt
     7921 agttacttga tcgtgagcag gtggttcttc gtcttctggt tctgcttttt cgaataattc
     7981 cattacagat tctagaggta aatctttcaa ttccgaccat tcgattggat cgtttaattc
     8041 tgtatcagga aagactgcat cttcagttga tttatcctgc atagctgcat ctgtatttgt
     8101 ttcttcaatt gattctttta tttgctgatc ttctgcagtt atttgatgac cgcagtctgg
     8161 acagatagta ttctgctcca tttttttcga tccgcaattt ggacattttt tcatcactta
     8221 gcgatcggct cctttactag gtttacgcta ccatttaatc tatcatattc tatacgtcac
     8281 gccaatagct tatttttagg atttacttaa aaataatgaa tgaatttcct tcctatagaa
     8341 aaaaaagctt gagactgcgt agtctcaagc tttttttctt ttaaaataaa ccaaacagtt
     8401 ttactgattt ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt
     8461 tgaacgagaa ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc
     8521 ccgagtaaca ccaaatactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa
     8581 accaaaacgt aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc
     8641 caattgttct tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc
     8701 ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac
     8761 aggttcttgc gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc
     8821 accgatttct tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg
     8881 tatcaattta ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc
     8941 agcgattgct cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa
     9001 tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat
     9061 caagtcaagg aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg
     9121 aaggtttgct tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc
     9181 taacgctact tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat
     9241 acgaaccggg tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg
     9301 ttcagctttt ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac
     9361 cacactgata cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa
     9421 agtgaatggt gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt
     9481 gttttctttg atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc
     9541 cataaaagaa ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg
     9601 atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc
     9661 tgcatttgtt tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc
     9721 aagtcttgga tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc
     9781 accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca
     9841 taatgttgtg agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata
     9901 aactgaaatt ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat
     9961 aatagaatct gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct
    10021 tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc
    10081 cttcgaagat cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac
    10141 agttgagata gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt
    10201 aattcttcga ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa
    10261 taacgtgatt taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat
    10321 gcttctccat acttacggag atattcatca ggatcaagct tttctggaac gactacgaca
    10381 gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa
    10441 cctgcgttgt ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg
    10501 atctgttgag ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc
    10561 catgcagcaa tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata
    10621 tcgcttcttg ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct
    10681 ggactattca agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt
    10741 cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa
    10801 aaacgatcag acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat
    10861 tcatttgaaa ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca
    10921 aaacctatgt tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct
    10981 aaagctggct gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt
    11041 tcatgagcag caatcagttt ccccgtagca gattcaggat tttggacagc tatagtacgc
    11101 tgacgccatt gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct
    11161 ggaaaactta gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa
    11221 ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca
    11281 tgaaaaggac acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca
    11341 atcacctcaa cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt
    11401 tgagccattt tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc
    11461 aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa
    11521 ttatacatgt ttgattgtat catatttcat tgctttttca agtataacac atttctatct
    11581 cattaagcat caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta
    11641 caatactcga agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc
    11701 caaccctaag tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac
    11761 gatcgtcgtt actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac
    11821 tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc
    11881 ccatgcgaat gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc
    11941 atttggtaaa gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc
    12001 ttgttcaaaa gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat
    12061 ttggataggg ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa
    12121 tgaataaatc gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg
    12181 gcgtccttca aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt
    12241 cgcaatctga cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct
    12301 taacgccaaa acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt
    12361 caattcattt accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg
    12421 atgagcagct tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc
    12481 ataattcggt aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga
    12541 aatcactttc agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt
    12601 tcggattgga tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc
    12661 gttcaattgc tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc
    12721 tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta
    12781 cacgactccc agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac
    12841 tttgtttgtc acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt
    12901 gatgttgtta tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc
    12961 gtacagcgga tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc
    13021 tgttttatcc aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg
    13081 aacaaggatt cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag
    13141 caataccaaa ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat
    13201 tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat
    13261 aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat
    13321 agctttgtga gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg
    13381 gataacttca gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc
    13441 agtttctttg acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg
    13501 tccagatata acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc
    13561 tcctaatacc agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac
    13621 ttctgattgg gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata
    13681 aattccaata acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc
    13741 tacaaataac agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt
    13801 atcgctgaat atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc
    13861 agaagccatt ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat
    13921 tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa
    13981 caacttgttt ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca
    14041 acctttttgt ttgaatcacc cgtgtgctgt ttttattatc aatctatctt tacttatatt
    14101 gttcctccaa acggctcatc caccaaccta gtgctgtacc ggctataaaa agaacaggac
    14161 aagctagtac cccagcaact cccaatcccg cgtaatcggt aggaatgatc ataatcgttg
    14221 aagcaaaaac tacacctaaa atgaaatgga atagcttgct atatgccttt gagaaaatgt
    14281 aatccatgat ttttgacaat ccaagaacac aaagaatccc accaattccg atcggcacaa
    14341 tcacttcaag tcgtacttct ttaaatccgt cagccatcgc tttatacaat cccatataca
    14401 ccaaaaaatt agaagggctc aatcctggga cgatcatacc caaaccaatc aatccgccgg
    14461 caatcagcca tgttccaaaa ttctgatcga cttgcccgtc aaataattga gcgccgtata
    14521 ataaaaacaa ataagccaga acaaaagtaa caaccatgat gatcacatca gaagtatctc
    14581 ttcctttttt ccctgcttct ttccataacg caggaacagt tccagcgata catccgacaa
    14641 aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa actgactaaa aaagaaagta
    14701 gaaagatgcc tgtcactccg cctagaccaa caggcagaaa aaacaaaaca ttttctttga
    14761 aattttttgt aaggtgggct aaaaaagaaa tcagccgctc atacatgcca aagacagctg
    14821 ctaatgcacc accgctgact cctggcaaga taaatcctga accgataaac attcccttca
    14881 caaaccgtaa catccagtct ttggttgtgt accccacttc ttgattttcc attttttgcc
    14941 ttttttccac tatttgcgta ctccttcttt catttaattt tattcatgat tgaacatttc
    15001 ctgctatttt acttaaagac tttttcatag gtctctatta ttttttcatt cattttgaca
    15061 atatcaggat aaaacatgtt acctccatta tgatagagat agccgccatt gtaaagcaag
    15121 gactggatcc gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc
    15181 acatctttag aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca
    15241 taggcaatat aatctttccc aaaattatat gcttggacag ctgtccataa atcgcatcct
    15301 tgtttctttg cttctactat catttccgcc aaatatttga caccttgatc aatactgtca
    15361 gctggattat caatgatctc agcttctcca tgcagacttt ccgagctctg catgagatct
    15421 gtcccttttc cttttgattc ggtcaatatt atcgccttga tcaaattctc gtagttttcc
    15481 atatgataat tttttgcagc tgttgttatt tctatttgat agctttccac tcgttttacc
    15541 gaacgataag ttcttcctca taaaaacaac accgcaccaa taaccgtcag aatgatcatg
    15601 taaaaaagaa tacggataat agataatcgt tttcgcttca tttcatcacc tagttatttt
    15661 gacatctttt tttatcttac tatttcttta tcttcttggc tatttcttcc ttgatttctg
    15721 tgtacgcctt aagaaaaaat gaattttcct gtattttttg acatcttacg aaaatgatcg
    15781 ttgtttttca gtaaaaacaa ttgcccaatg actgatataa tcgttatact ttacacagta
    15841 ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag cgaaatttac
    15901 tatgaaaaga gcggtcaagg cttccctctc tttttactgc atggcaatga cggcagcggc
    15961 cgcttttttt cagaacaggt ccctgtactc gaacgctact ataccgtgta tctagtagat
    16021 agccgaggac atggtcgttc gacaaacgaa gcatctatgt tgaattttca gttaatggcc
    16081 gaggatctga atacgatcat gttgctggaa aaaatcgatc aggcagattt tcttggattt
    16141 agtgatggag caaatctagc actggtattt gccagcagct tccctaaaaa agttcatcga
    16201 ttgattttaa attctggaaa cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc
    16261 agtaatattc attatgcatg ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa
    16321 aatcttttag ttatcaaact tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa
    16381 aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat
    16441 tctttgtata tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa agaacaagga
    16501 catgaattag ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca atttctttct
    16561 gaaacataac tattaggaat agaaggtgat ttatttgtta aaaaatacca tacaatggct
    16621 gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt tctgtcattg tcattattgt
    16681 cggtgaattg atgtccatcg gtaaaactct atctatccag caattaggag aaacgtttgc
    16741 cactatacct gtatggaaaa gcggactaat gctattgatt ggtcttatct ctgtgttacc
    16801 tatggttggt tatgatatta ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta
    16861 cctgtttgag acaagctggc tgatcaatac gatcaacaat attgccggtt tcggcgggtt
    16921 tgttagtgtg gggcttcgct ctgaactata tggacaaaag aaagagagca gaaacgtgat
    16981 ccaagcatta tcgaaagttt tcttgttttt gatggctgga ctttctattt acagtctgct
    17041 ttctttttta cttgtagtct tcacgccagt tgctcctttt ttgaagcaat attggatatg
    17101 gttgatcggc ggcggtctat attttcctgt cgtcttttta tttactactt tcaaaaagaa
    17161 aggattcctt ggtggtacct cacttaaaac tcaaggacag ctattgctag tttcattgtt
    17221 ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg aaatccggat
    17281 tgatctgtgg caaacaattc cattattcat tgctgcttca gtgataggaa tcgtgtctat
    17341 gattccagga gaaattggga gtttcgatgt aatgatgatt atcgggttgt ccgctattgg
    17401 tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat cgcctgttct actacatcat
    17461 tccgtttttg attggaatcg tctttttctt caaaaatatc ggttccactt ttgatcagcg
    17521 ttattctggt atccctaaac aattagcaac agaaattgcg cataaaatcg tggttgtctt
    17581 attatacttt tctggaatca tgctagtact ttctgccact atcccgcaag cttttacaga
    17641 gtttcgctgg cttcatagtt tgaacccatt gaaatttcat tttattatcc aatttcctag
    17701 tatactattg ggatttcttt taatcattat gggcagaggg atcgcagcac gtgtgaaacg
    17761 agcatatttg cctacaatct tcttgattgt tctagcttta ctttatgttt tattaagtga
    17821 ttttagtttc acaccggtgg ttttcttgtc tattctgctt ctgatcattc ttgcttctaa
    17881 aaacgaatta tttcgggaac aattgatcta ttcatgggag tggcgtacga tcgatggaat
    17941 cattattggt gcactgtcct tgctttacat cgttattggg gtatacaatc tgccggattt
    18001 ccctcatcgg cgtcaccatt ttatttcctt tttcttattt ccttctgaaa agatttggtt
    18061 ttcgggattg ttggccatca tcgctgttag tttcatgatc gttctatttg ttcatttcct
    18121 tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct
    18181 tacaacttat ggaggaaact ccgatagtca attgatcttc ttaaaagata agcggatgtt
    18241 tgcctatgaa aaagacggtg aaccaactgt tcttctccaa ttcgcttgct tcaataataa
    18301 atgtattgta atgggtgatc cttctggtaa gaaagaagat tttccagaag caatcgaagc
    18361 ttttatcgaa gaaacagatc gactatgcta tttaccagtt ttctacgaaa caagcgaaga
    18421 gattgttatg attttgcatg aattcggcta cgatttcatt aagatggggg aagaagctta
    18481 tgtagacctg aatagtttta caacttcagg gaaaaaaatg aagggcactc gtgctgtctt
    18541 gaatcggatc gaacgagaag gatttacatt tgatgtctta caaccaccat tttctactga
    18601 gcaaatgagt actttcaaaa atatctctga taattggttg ggttcacgaa aggaaaaagg
    18661 gttttcttta ggctttttct ctgaagatta tctacaaaga gcgcctattg cagttgtcaa
    18721 aaatacccaa gaagaaattg tcgcttttgc aacgatcatg cctacttaca cgaacaataa
    18781 agtaggaacg attgatctga tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga
    18841 tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa catatccaat ggttcaattt
    18901 agggatggcc cctctttcaa atgtcggaac ttccagaaag agttttttac aggaaaggat
    18961 tgcatacttg gtttacgagt ttggttctca tttttattct ttccatggat taagagaata
    19021 taaaagcaag tacgctacaa actgggtctc acgttatact ctatattcta gagatagttg
    19081 gattgcatat gttatgattg ctttgctgat tattgataat gcgccagttg agcagacaca
    19141 aggtagaata agtggtttga aaaaatggat acgaagaaag tattaaaaac tgcataccga
    19201 tagatgaaca ctctcatgat gaataaaaga gactgaagta aaacgaaagt tcatgcttct
    19261 acaatctctt cttatacaat tgaagaacaa agagcggtcc aaacagaaat cctgtttgga
    19321 ccgctctttt attttaaaca tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct
    19381 tcgatctctg taacgatacc tgaacctact gtccgtccgc cttcacgaat tgagaatgtc
    19441 gttccgtttt caacagcgat tggatggatc aagtcaacat caatcgtcac gttgtcacca
    19501 ggcatgacca tttctgtatc ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg
    19561 aagtagaact gtggacgata gttgttgaag aatggagtat gacgtccacc ttcttctttt
    19621 gtcaacacgt acacttctgc tttgaatttt gtatgtggag taatggaacc tggtttagca
    19681 agtacttgtc cacgttcgat atcgtcacgt tggatccccc gtaacaacac gcctacgtta
    19741 tccccagctt ccccgtaatc taacgtttta cggaacattt ctacacctgt tacaactgct
    19801 ttttgtgttt caggtttgat ccctacgatc tctacctcat caccgacacg aacagcacca
    19861 cgatcaatac gacctgaggc aacagttcct cgacctgtaa ttgagaagac gtcttccact
    19921 ggcaatagta atggtttatc tgtatcacgt tctggtgttg ggatatattc atctactgta
    19981 tccatcaatt ccataatagc agcttcagca tctggatcgc cttgcaatgc ttttaatgcg
    20041 gaacctttga tcacaggagt gtcatcgcct gggaaaccat attcgctcaa taactcgcga
    20101 acttccattt ctaccaaatc gatcaattct tcatcatcga ctaaatcaac tttgttcaag
    20161 aaaacaatca ggtattttac accaacttgg cgtgacaata aaatgtgttc acgtgtttga
    20221 ggcatcggac cgtcagttgc agaaacaact aagattgcac catccatttg tgctgcccct
    20281 gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt
    20341 ttttctgttt catattctac gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct
    20401 ggagctgcat caatactagc atagtcttga ggatttgcta ggcctttttt gcctaatact
    20461 gtagtgatcg cagcagttaa agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg
    20521 ttaacgtgtg gtttacttct gtcataatgt tcttttgcca taatttacaa acctcctaaa
    20581 taatatagat cactgaaaaa tttcagctct caccttttta ttatagtctt tttttgacta
    20641 aatcaaaaag aatacgtccg acttactttt tgtaagattc gaactaagct tattatacgt
    20701 caatattggt caaagtcaac taagagcact cgtctgtctt atacgcgaaa aaagtacctt
    20761 ttataaataa aaaaatgtgt tttttgagaa aaacttcaag atagtcctta actttctttt
    20821 gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa
    20881 tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta
    20941 attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat
    21001 ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc
    21061 caagcagata acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa
    21121 gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat
    21181 gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag
    21241 gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa
    21301 ttttattaga taaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt
    21361 tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt
    21421 tgtctcacgc tctttttttg ttatattcgt cttttccgat ccttctcttg acacaattca
    21481 tttaactgat cactggtact tgaacaccta atattctagt catttttata aacgattcaa
    21541 tacttcatcc acatttttga tcatcacgga tatcgtacca tccggattat tcacaaattc
    21601 aatcaaatct gggttgcgat atacatctac tggtacgatc agttcgatcc cgtttgatag
    21661 cttgaatttt tgttttccaa attttttctc agaaatttct tgtacttctt ttacaggagg
    21721 tgcttctggg acaaatcctt gctcttgtac ttcttcttta aaagccattt ttgccgtgat
    21781 gttttccttg aagacttttt ccgcaatcat ctcatgatcc aaccgacctt tttcttctat
    21841 cgtatcatag acagcttctt tcaaatcagc catgatatca aattcttctg tctcaaattt
    21901 ttttccaaga tgcttggcta ctttttggat cacttttaca ttttcttcta aagaaggtgc
    21961 aggaacactt tcgatcactc ttccagaaaa ataaagctct tttttgccag aaaactcata
    22021 ttttttttca attaattcgt aggtccaatc agtcaaattc acagcgaatg cttcatctgc
    22081 tttttgtgtt ttcgccccta gaattgctcg attcaaaatc aagcggttgt cgatccctgc
    22141 ttcgtctgct tcaacaaagt gagtgtaagc ctcacgataa ttgactttca ataaagataa
    22201 gtacatcact gtatctaatt catacaagac aacaaataca tctgcactag gtgcttcttc
    22261 actttcttgg tagatatcga accaccgctg aacgattgct tcacttgctt ccacaaaatt
    22321 ttcttgagcg attttaagtg attcaacgat catgctttct tctgctagtt gtccagtttt
    22381 tgtttgagca gaagagattt tttggatctt cttttgaaga aaatcacgaa tatattcttt
    22441 tgtcagatcc agctcttttt gtgaaaagac tggagaacca gtttctctgt caataatatg
    22501 cagaattgct ttttttaaat agatatccat tttctcattc ccttctgtct tagtttacgg
    22561 aaaaaaaatc aaaaatacca gaatcaaaat cattttcttt taaacaaaaa tttcaagagt
    22621 agaaacaaaa tggattcttt ttttcgaaaa atgcgctaca cttatagttg aacgaataaa
    22681 aaaggagctg aaagtatgaa ttcattaaca agcacttatc gtctatcaaa tggttacgaa
    22741 atcccggtag taggattcgg cacatggcaa acacctgatg gggatgtcgc tgtttcctct
    22801 gttaaagaag cgctggctgc aggttatcgc catatcgata ctgcccaagg atacaagaat
    22861 gaagaaagcg tcggccaagc gattaaggaa agtggaattc ctagagaaga gatattcttg
    22921 acaacaaaat tatggaatgc aaaccacagc tatgaacttg tgatgtcttc ttttgaagaa
    22981 tcattgaaga agctccaaac agattattta gatctgtttt tgatccactg gccaaaccct
    23041 gttgcttttc gtgataattg ggaacaagca aatgcagata catggcgagc atttgaagaa
    23101 ctgtatgaag caggtaaaat caaagcaatc ggtgtgagca atttcttacc ccaccatcta
    23161 gatacactag caaaaacagc aaaaattatg ccgatggtca atcaagtttt ccttgcacca
    23221 ggcgaattac agacaactgt agttgagtat gcaaaaaaac atgatatgat cctcgaagca
    23281 tatagtccat taggcactgg taaaatcttc gatgtgcctg aaatgaaaca aatcgctgaa
    23341 gcacatgaca agacgattgc acaagttgct ttgcgttggt cgctgcaaca cgaattcctt
    23401 cctcttccta agtcagtgac accaagcaga atcaaagaaa atactgaatt atttgatttt
    23461 gaattaacag aagaagaaat gaaacaaatc gatcagttag atggtgtagt cggcaaagca
    23521 aaagacccag atactaccca attttaagtc gaaaggtgat tttatggatc caatcgaacg
    23581 cttgaattct ctctcggaag aagttaccca gacttttcat tccgattttg tctttttgat
    23641 cgatgcagag aaaatccaac attttcctgc aagaaactgg acacatgatc aaatcatcga
    23701 agaactgaaa aaacggttcg atcattcgct aatggtcaaa ccctggcacg aacatgaagt
    23761 catttattca cctgaactac ctgtttttgc cttgataccg aaaaaataga aagaatggaa
    23821 cggattatgt atatatacat aatccgttcc attttttatt tttctttcaa aaaacgggaa
    23881 aatacgcaaa tttttctgtt gtaatttgcg tatacgcaag tttttttgca ttactccttt
    23941 tcatttggct gtttttttct tataatcgaa agggtatgag cgaaggagag aacatatgaa
    24001 gaaaaaacag aaacaaaaat tattaaatca attccgtcct tcattggatg gtgtaagagg
    24061 acaattattc cgttctctag aagaagagag cttgcgacgt tatggtttgc cgttacaagt
    24121 catgcttgat cccaaaaacc gtcaaatctt aacgatcgga ttagctggga aaacaaatga
    24181 agatacacgg atcaacgtgc caatcgatga caacttcaat actgtattaa agagaattcg
    24241 ttcaggagaa aacgggattt tcgaacgatt ccgtgacaat cttcttattg aaatcgtgtc
    24301 ttactggaat gatcaacgat tgaaaaacga tgaaccaact gcgcaagctg ttgcgacacc
    24361 agttacagaa gaaacagtca gtgtgccaga agaaaaaacg gaaaaaccaa aagcggacac
    24421 aactgaaaat acacgctcaa ctgatacgaa tgccgcttta agttttgctg atttttcaaa
    24481 agccgttgct gagtatccta aattttacac agaacaaaat gaacatgaag tagttattaa
    24541 tgaaaaaaat gctgatgaac ctcgtaaatt agcaactatt tctatgacta ctgaaaatga
    24601 gttcgttatc gaaaaagcac tcgaacggaa atacaaagtc aaattgactt tgattccttt
    24661 gatcgaacaa tttgcagcga cagcgattac tgatcgataa aaattctatt tgtcataaaa
    24721 ggctgggatt taaaatccca gcctttttag tcatataata atttctacag caagtaaaaa
    24781 gaaattcctt ctaacatcag gctcaaatat cagcttgacc tgtccaacaa atcacctatg
    24841 atcctacaat gttaggcgac ttctcttcta cctgaagata tcttggagtt gcctgatttc
    24901 ttaagatgcc taaccttaac tataaaaaat tctaataata aacatatttt ctgatagaga
    24961 aaaaag
//
LOCUS       DAZAJZ010000040        24538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000040 DAZAJZ010000000
VERSION     DAZAJZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.040"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..250)
                     /locus_tag="ACG86D_001504"
                     /pseudo
     CDS             complement(59..250)
                     /locus_tag="ACG86D_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(618..959)
                     /locus_tag="ACG86D_001505"
     CDS             complement(618..959)
                     /locus_tag="ACG86D_001505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140649.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(1056..1508)
                     /locus_tag="ACG86D_001506"
     CDS             complement(1056..1508)
                     /locus_tag="ACG86D_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140650.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(1515..2063)
                     /locus_tag="ACG86D_001507"
     CDS             complement(1515..2063)
                     /locus_tag="ACG86D_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFN3140651.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(2108..2440)
                     /locus_tag="ACG86D_001508"
     CDS             complement(2108..2440)
                     /locus_tag="ACG86D_001508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140652.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(2454..2711)
                     /locus_tag="ACG86D_001509"
     CDS             complement(2454..2711)
                     /locus_tag="ACG86D_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140653.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2906..3448)
                     /locus_tag="ACG86D_001510"
     CDS             complement(2906..3448)
                     /locus_tag="ACG86D_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFN3140654.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(3469..4560)
                     /locus_tag="ACG86D_001511"
     CDS             complement(3469..4560)
                     /locus_tag="ACG86D_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3140655.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(4568..6778)
                     /locus_tag="ACG86D_001512"
     CDS             complement(4568..6778)
                     /locus_tag="ACG86D_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN3140656.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(6794..9322)
                     /locus_tag="ACG86D_001513"
     CDS             complement(6794..9322)
                     /locus_tag="ACG86D_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3140657.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(9374..9784)
                     /locus_tag="ACG86D_001514"
     CDS             complement(9374..9784)
                     /locus_tag="ACG86D_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN3140658.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(9797..10015)
                     /locus_tag="ACG86D_001515"
     CDS             complement(9797..10015)
                     /locus_tag="ACG86D_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFN3140659.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(10012..11004)
                     /locus_tag="ACG86D_001516"
     CDS             complement(10012..11004)
                     /locus_tag="ACG86D_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3140660.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(11083..11994)
                     /locus_tag="ACG86D_001517"
     CDS             complement(11083..11994)
                     /locus_tag="ACG86D_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3140661.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(12013..12357)
                     /locus_tag="ACG86D_001518"
     CDS             complement(12013..12357)
                     /locus_tag="ACG86D_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140662.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(12377..12655)
                     /locus_tag="ACG86D_001519"
     CDS             complement(12377..12655)
                     /locus_tag="ACG86D_001519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140663.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(13014..13514)
                     /locus_tag="ACG86D_001520"
     CDS             complement(13014..13514)
                     /locus_tag="ACG86D_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140664.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(13515..14060)
                     /locus_tag="ACG86D_001521"
     CDS             complement(13515..14060)
                     /locus_tag="ACG86D_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140665.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(14057..15313)
                     /locus_tag="ACG86D_001522"
     CDS             complement(14057..15313)
                     /locus_tag="ACG86D_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3140666.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(15673..15984)
                     /locus_tag="ACG86D_001523"
     CDS             complement(15673..15984)
                     /locus_tag="ACG86D_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140667.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(16007..17482)
                     /locus_tag="ACG86D_001524"
     CDS             complement(16007..17482)
                     /locus_tag="ACG86D_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFN3140668.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(17931..18383)
                     /locus_tag="ACG86D_001525"
     CDS             complement(17931..18383)
                     /locus_tag="ACG86D_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140669.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(18385..18750)
                     /locus_tag="ACG86D_001526"
     CDS             complement(18385..18750)
                     /locus_tag="ACG86D_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140670.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(18849..23132)
                     /locus_tag="ACG86D_001527"
     CDS             complement(18849..23132)
                     /locus_tag="ACG86D_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFN3140671.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(23279..23518)
                     /locus_tag="ACG86D_001528"
     CDS             complement(23279..23518)
                     /locus_tag="ACG86D_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140672.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(23608..23850)
                     /locus_tag="ACG86D_001529"
                     /pseudo
     CDS             complement(23608..23850)
                     /locus_tag="ACG86D_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23963..24199)
                     /locus_tag="ACG86D_001530"
     CDS             complement(23963..24199)
                     /locus_tag="ACG86D_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140673.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         7308 a         5235 c         3553 g         8442 t
ORIGIN      
        1 ttgtttgtac tttttacatt ggataactaa ttatgactgc tgataacttt tgatattaaa
       61 ctagtatctc caatacaaat aaaaaataga tatcttgttc atcaaaatta gttcgtggat
      121 acttaccttc atttacatca ttcagcggtt tcccattttc ttttattcta ttatttcatt
      181 ttctaataga cttggcttca tttcgcaata ctttttttca ataggcatca aattggtgtt
      241 gtttagatat ttagttgtag ttttgtgaca tgataagtat gctccttttt ctcttttcca
      301 ctaatacaac gatcaaaggg tgcttttttc aaaaaactag aaaaatcaaa ataagattcg
      361 tattttgatt ctatatatcc tgtttcaact acatcttata tttcacaata gaataaatag
      421 ccttttgcat agctgcttaa cttataagaa attgcaattt acttcttcaa agtgaaaacg
      481 aatatgagta aaataaaatt attcttctta cagataatta aaaatagcgc gtaacaaaaa
      541 tcggcattca atcacataat taggattgat gccgattact tatattctac gtatagtttt
      601 agtattctta ttttttatca ccagtaacca attgagaaca gccctaaagc agaatagtct
      661 tttatctcaa aacgcttaat ttcaggctta ggaatatgaa taatcttttg agtatattct
      721 ttctctttta atcttactga agaaatacca ctgcctatct tttttacaaa tatttcattt
      781 ggtgtacaga ctaataaaac aatatttgta atcaatcctt gcactaaaga attaaaagga
      841 agactattta aaacagggtg tgcataacta taacatgtga agtagttatt ttgaacttta
      901 acttttaaaa actttaaaaa taatcttatt tgttcttcta aacaatcatc tatactcata
      961 taatcactcc actaattaat ctccccaaaa agacacagca ttaagagata taccaacaca
     1021 acagtgcaaa taactctaca tcttccttcg caaccttatt tcaataaacc ataaaaatta
     1081 ttttttaata gaaactgaaa attaaaacta gaaaaagtag ttttaccgaa tgtaagagca
     1141 tcatcaccat caatatagaa atacattctt ttccagttac atttaatttt taaacaaaat
     1201 tctccccaaa aaggaacttt ttctagtgaa aacttggtaa tctctttgta attaattctt
     1261 gttaaagaat aattaaacac ttcattttta cttgttattt tttcattgac tataactagt
     1321 tcatctggta aaaaaattaa ccaacaaaca tcgacaaaca gtaaatgata taatccattt
     1381 ccacctccaa gcatattcaa tactggtgaa catgccttag aaacacgaat atagttatgg
     1441 tttaaataga tattttcttt aaacattgct tgtttaaaag ccatatgctc ttgttctaaa
     1501 cttttcataa acttttactc taatgcacag ctagcagcag ctgctgcaat tcctgctaca
     1561 atatttacct tagctaactc tttagctgca gcttttccta attgtttttc tgcaattttt
     1621 aataataaat ccgcagcttt ttcaaaatta aaagctttta atgcttcaat aaaatctccc
     1681 tgcgtaccta tagcccaagc cactcctcct ccaggaagag aattaatggc tacacatcca
     1741 gcatatcgtt tccaatcaac tttctccatt ctaggaacaa taatatgttc ataaaagact
     1801 cttgctgctc ttccttgttc gttatcttga ctgattcgtt cacgaatagc accaaaatcc
     1861 tttatatggt atacaccatg ttcgtctaaa aaaccaactg tatcaaaata aaaagttaat
     1921 tcatctacta atttattctt tatttctaaa tcaactgtat tcactttttc tgtggcatac
     1981 acacttgttc cagtactaac aaaagtactt aaacctaata agaaaacacc taaaacacca
     2041 aatattttaa ttattctttt cataatttct cctccataaa aaataagtta ctttttaaat
     2101 atcttgttta tgaattcatt ccccaaaggc cttttaagat tgatacagca gtccgttcac
     2161 ttcttttaga cttgtcatac aagtctttta aatgatccca tatcagacta gtttgtctat
     2221 ctagcttcaa tccattaaac gctaatgaac ggttcatttt ttctattact ttagataaag
     2281 ctacagaaat ttttatacct tcacaacgcg catcttggat ttcaataata gtatcatcaa
     2341 ttagtttttg aatttcttgc ctatcaacat attcaatctt agaaagagaa tcttttaaag
     2401 ctactaatga ttcaacgatt tcttttttct cttcagacat tttcttcctc cttctagtga
     2461 cccgtaaagc catgtagagc agagtccatt gaaccagtaa ttacactatc tacaacgcca
     2521 ttccaattta ctctacattt tccatttttc agatttgtcg gtccatacgg atagcctttt
     2581 tgacattcaa tgttcggatt ataccagata ggattatacc attcagccgc agaagcagat
     2641 tcagtcgata caaacaatcc cacaccaaca aatccaatac acatcacact atagccaatt
     2701 agttttttca tctcgtatcc atctcctaaa aaatagaata gttcttatag ttattcataa
     2761 gacatatttt ttccgttttt gttttatcct ttctgtccta taacaacttt tttcaagtaa
     2821 agaatgacat agtttttctt aaaaaaacag tctgtttttt tctatttctt cggtgtataa
     2881 taaatatgag tgttcatttt ttattttaat acgcactccg tacataataa atcttgatcg
     2941 catcaatctt ttgagcttct tgacctactt tcaattgaag taatacaaat tgagtaacct
     3001 tattcattgt ttcttctcca aataacaaat attgatccgg attctctata tcttgtgtca
     3061 cctggtaaag tttcccagtt gttttcattt gtacatgcgg gtctacatca atactattgt
     3121 ctttaataca acgatctgtc atatagtccc tcacagcttg attgcgttta taaacatctg
     3181 tgaaattact ccattttcta gcaaaatctt ttaagaaatt ttttgtcatt tcatctattt
     3241 gcatttgttg cgctgtctcg tcttctaatg aaccaatttg actgttactt tcagtacttt
     3301 gaagagttcc acttgtttct tttactgctt cgattgtttc ttctgtcgtt ctcaattgtg
     3361 ttttttgttt ttcagaagaa aaccaatgac aaccagtaag acctatcaaa cataaccaac
     3421 taaatactaa aattatccct tttttcacgt ttctagtcac tgcctttctt atttcacctt
     3481 acgaaccaga ctacgaattt ttgtttgatt gtacgtgcgt tgataccgag ccataatccg
     3541 tccttgctcg gcattctgtt ccacactaga gaaatgttgt ccgccatatg aaacatctgt
     3601 tataattccg gtgtgaccat agattccagg agacaatgca ccacctgctt gccaattcac
     3661 cacatctcct gcttgtaaat cacttggttt tgggttcatg attacttgcc aaccataggt
     3721 tgtccagtta taatcttccc ctattaacat cgcaaacatg tgtccagaac ccattaactg
     3781 tgggccacct aattgagata cataccatgc cgtaccacca taacattccc cattatttac
     3841 ggatcgtcca atgatccgat ccaaaacctt cgcattgcct ttactttttc ctgcaataag
     3901 cgaagttttt tttgttggta ggctattcca atgcttgatc gcaaaagtat catattccgt
     3961 taagttatag gacgttatga tactcattgc tcctcgataa tacggactcc cttcggtaat
     4021 acctgctgcc gcatagcctc cacgacctag aatcaataaa caacttccag gatctttatt
     4081 gcccacacat tgattaaagt tgctttggct cgccaccata aagccataat agtcataaca
     4141 agcttttaag ttcggaaact tcatatagaa accaccttca gaaccaccaa ttccacgtgc
     4201 agaaccttgt gggtagggac aaccaggaaa ccaggtaatc ccaaaaaagt tcgtatcgtt
     4261 tcttccagat tgtgactgtc cataagctga ttcatagcct aattgagaaa gaatcacact
     4321 cggtaataac tgatacttta cagctgcacc aactgctgct tgcgcatgtt catctgtgat
     4381 tggaccacgt cctttaatct ctgaaaaaat cggtaatcct tcggtatatt ccccattaaa
     4441 agcgacagct agacctgtag cttctcctcc atgctcagaa agttctcctg aaaaaaagaa
     4501 tggcaataga aaaaagggaa gaataaagaa aagaagacaa cctagacacc ctttcttcat
     4561 ttcttcctca tcctttcttc gataatggtt tttggactgt tcgtcctttt ggtaaagtaa
     4621 aatcagttcc tggttttgcg tccaaaatcg gcagtgtttt aggatccttt tctatttttt
     4681 ccgattgctc tgttggctta tctaaagaag aaaatgcagg ttgtaacgat tttggtgcgg
     4741 aaatcgacgg tttactttca gacgaacgtt gccccggttg ttgcacagaa cgatttcctg
     4801 atagagtggc taccgacgaa ttaggaacag agtattggtt caataactgc tgcattgctg
     4861 gataactccc atttttcgcc tgatttttca cttggcgaat ctgttgtgaa agagaaccag
     4921 cagctgttaa ttgtggcacg ggtacttttc cagaaggcaa cggaatcgat tgaccaacaa
     4981 aagcgatacc tttatttaac aatggttttc tttgattatt cccttgttgc ttactcaatt
     5041 gtgccaatga ttgtacctgt tgttttggtc ttactgaatt catggacgaa tttggaatca
     5101 aaccatttga aagaccagaa ttccactctg ttttcgattt ggtagaagac atttcaggct
     5161 ctaccccatg ataacgaaca tgacgaccac tataaggcaa ggcttgattt gaacgcccac
     5221 ctagttcaaa gggattaacc acttgcctcc cttttaccga ttcttgtggt gtacgttttt
     5281 gttgaaactt ttgaaatcca tgtatcgctg cagaagcgag tggattcgtt gctcgataca
     5341 cattccctgc taatcctgca cctttgattg ccaaattttg tccagcagtt gatagttgac
     5401 caggcagttg tttcatttga gacaaagaat gacttgctat gtttttcgtg ttgtttatag
     5461 tttgatctgc agctcctaac cctagtttct ttaataaatc tttccgtttt acaatgagta
     5521 aaattgatag gagtgctttg acaaacatat ttcctaaaaa accaaaaaca gtattcattg
     5581 gaattaaatc gtctactatg ttatagatca aaaatgccac caaaataatc acactgtaaa
     5641 aagatttttg aaacatcaag cccaaaaagg actttaaggt attcatgagc atataatcaa
     5701 atactggaac aaaagaagcg agtaatgaaa aaggaatcac aagtaataaa cacaacacca
     5761 acatttgaaa gataaaacga atcatgccaa tcactacaag tggtaatcca atacttagat
     5821 taagcaatgg caccatagaa ccaacgactg ctttataaac agctttagtt ggttgaatat
     5881 agacacgata agccttcttt ttccctctat cttctcctgc agcagtggtc acttttgtct
     5941 caatatcaga taattcttca cttgacgctt ccttggctag aaattcttgt ggttcaatct
     6001 ctgcttgata caattcttct aaatttgttg tcccataatt taacaatata tacggatcaa
     6061 caacagccgc tttataataa agttggcgca ctttttgaat ggcttccttt tgtgtcgttg
     6121 gcactaaaac accattgaca tcttttgtcg ccaattcatc tagactattt gtagcagaaa
     6181 aaataaagcc ttctacctcc acggacaaat catttaaccc tttggtaaaa gcttctcctt
     6241 tactaggtac gttactacca tagccaaacc aaaccattga aaaaatgaca acgaagagaa
     6301 atcgaataaa caccattttt gcgtaatgtt tccctctaaa tatacgaaga gtaaatagat
     6361 ataacacata caagacgaca atccccgcac caataggatc gtatattttt gaataaatag
     6421 tatccgaaaa agtgaaaaac tggtgaatta actcgttcat tgcagctcct tcatagagct
     6481 tctcaatgac aaaatcaagg ccttgccaaa tcaatttact taaactaaag aacatattcg
     6541 tcatcatttt tagaaaatct gtgccatttg ccccaaataa cattttgcct aatgaaaatt
     6601 cactatcaga taaataagaa gaaaaggtat caatatgaaa tttcccaatc tcatagatcg
     6661 attgatccac ctgcttttct gccgcataca ccaatccacc aacaaaaagc agtccgaatc
     6721 caatcgttaa aaaaaacaga acgcaccatt tcttttcctt atgctttata taagccattg
     6781 tttcacctca tttctattgc atgattaacg tatcctgttc ttctacttga gagtgacttg
     6841 ttttttccgt tgtttgtaat gcagcggtca tttcttcaaa caaactgtgt acattgagtt
     6901 ttcctacacg tccataggga tctaacatcc aacactgtcc tttaatcatg tggctcattt
     6961 cagctaagtt cgcttcattt acttccaaac gtaaatgatt caaaatcttt tctcgctcac
     7021 tgtcttcatc aaaagcaaag acgacaccaa aattcccttt ttctttatct gaacctaagt
     7081 caccaataaa ttgtgtatct aacactaatt gattcatctg ggaacgcccc acacgtgcca
     7141 ttgctgcaat aatactacgt cctgcgttcg aattattaaa tacccatgct tctgtaaaat
     7201 aaatcgctgt tttcttacta ctatctcgtt ttccaaacat ttcacaaaaa cgacctaaac
     7261 taatcatcaa acacaaagac tttcgatccg catctgtata tagttctggt cggacattgt
     7321 catttggtaa tttcaaatct tggatctcta aaatcgttgt tttggcatta aagtctaatc
     7381 ccgggttttc tccataagaa aatcctaaac gtaaaatcga atcttctacc gtcaagcgta
     7441 aaaactcacc atattctcga acctcttttt gttccatttg ttctaaatag tcgaccacat
     7501 gcatcaagcc aacttgttgt ccggcttctt tttgaactaa aacttcttgg attcccttta
     7561 ataccgcccc ttttaaaagg tgattggtac ctaacggact taactcatca atcatatcct
     7621 gggctacttg tttcgcatct actcgggata aacaaacaat tggatctaat acaccataat
     7681 tacggggatt tgtagcatct aacgtgacat aactcatttt ttgcaaatgt tctacaaaca
     7741 aagggtaatg tttctgataa tacgggtctt gaatgagttt cgtaaaccat ttgcgctttt
     7801 ctcctttggg atccacaaat aaactttgta cattcataaa cgaactatac aaaaagagca
     7861 agccaactaa aaaggtcttc cctttaccgg tttttccagt tactgctaca tgaggcgcat
     7921 caaacaacgc acctttgatt ccttccgcaa tggctagaag attcaaaaag acaaaacgat
     7981 ttgacgcata aattaattct tctaatggtg ttttttctcc tcgagaacca ccatccccta
     8041 atgtatcttg ccgaccaata taccagccgg attccatacc taattctgat actgctgaaa
     8101 acaaccattc gccaactgct tctacagttg ttcggtgctt ccaatgtaac tgccgtccca
     8161 atggttgccc taataaacat ttatagaaca attctcgttg atcatttttt gcacggtgga
     8221 tatggataat cgtatgaagt tgttgaataa ccgttgcact attttgacga acttcttcaa
     8281 ccgtttgacc gtatacaata aaacatgcga tccaatcgac gacaggttgc ccattatcta
     8341 ctcgttgctt taaatgtttt actaatcctc gaacagttaa taagcgttca ctaggttctt
     8401 catcattttg aaccaattcc ttatgctctg ttttgaaccg ttccgatagc caattcagtt
     8461 ttaaaccaat acctccctgt cctttcaagc gtggatagtg aattttcatt cgcaattctg
     8521 ctgggaaaga aagatcctga gcaaattgaa atagatcaca atttctcata ttttcaggtg
     8581 tttccccaat aggcaacaat gctaaataac tactccctaa ctcatcttct aattgaagaa
     8641 ggcctttatg ttccttcggt atacgtacat acgttccttg attgtctttc agtacttccc
     8701 aattattttg ttcttctact ttgtgagcca ttcctcgaat atattgcaga cgattgagat
     8761 acaacagctc tttttttact aagggacgaa tagaaaagct ataaagtaat tgtgtcactt
     8821 cttttacata aggatcactt tcagaaaaca aagtttccaa atcatctact tctaatccaa
     8881 ataagcgtgc taaacttcct gctaaatcta accagattag atttattaca tcatgaaaac
     8941 tctccaccgt tgctttttct ttgactttga ttcctaaaat aaatgaagga cgatagactt
     9001 gaccttctcg ttccaacaat gtcactgttt cttttgcata atacgcacca attgcttgac
     9061 tatcgggaga aaatcccttt cctaatgtgt caaacttcgt ccgtaaattc attgttcgtg
     9121 gatacatcac caactccaca tcttcataat ctgctaaaag caacagtaaa cgagccatca
     9181 tttcttttat ttcctgtgtc ttttttggtg tgttttttga aattgttcgt ggaaaaacat
     9241 gatagtaggc ccagatacaa tcctctccat ttaacaaaca atttttcttg aatgctttta
     9301 ctggttcttt tacaagcacc aatgaatttc acctcccaat aaaaaaggaa caaccagttg
     9361 attgtccctt tttctaatcc tctaaataaa catacttatt cacaaaaagt aattcttcca
     9421 atgcattgtt tgataccttg tcccagttat aatacacaca atcaccattc atcatacgaa
     9481 tcatataaca aaaccaatcc cataaataaa aatgcacttt ttttccatca ggttttagtt
     9541 ttgccagtgc taacacacat aaatagggga gtcctatata aaaaacaact cctgctggtg
     9601 gaaaaaaaga aatgacccaa ccaataaatg aacgtaaaaa taaatattca aagattacga
     9661 ccaacaaaaa agttgctgca ttgactaatg caactggaaa tggaagacgc caattgcttg
     9721 taatctgttg tacgcgataa ggttccgcat aaacaatcga ataatcataa ggttcccaat
     9781 ccacttcact atcctcctaa tccataaata atttttcaat gattttacca aacgcatcaa
     9841 atacacgtgt tggatcattt aagaagaaat aaactgcggc acctgctgcg atagatatta
     9901 atgcatttcc tacttttgat ttcataaaat ttcgcaatcc cagataaaca accaccatta
     9961 ttaaaaaata gccaccttgc gttaatataa aatctacaat tcccttcagt gtcatttccg
    10021 ttcttctcct ttcctatcca accaattatg tgttaatttt tcaacataat actgatttgt
    10081 ttttttcgta atgacaatcg tcatttgttc tttttgtaaa agtgacgtat caccatctcg
    10141 aaagacgacc gttgctttta caataaaagg ttttttcgtt tctttataac gcaatacttt
    10201 catctctact tgatcaactt tagctgtttg attgagtcct tcaggttctt tcatcatgta
    10261 tgacatatct tttgcattgt ttttcgcata cttatctaag aaatcttcca caaatccttc
    10321 aacatctttc gcttccgacg catcaaccgt ttcgtataat gtgtcatcat aagtaatcat
    10381 tcgttgtttt tctgtattct ttggtagtgc cgtaaaataa ggataagcaa taatacgaca
    10441 gcccttgttt gaaatagaaa agggaagatt gagtacttgt gtgattgtca cacttgtttc
    10501 tttcttactc tctggattca tcgagcgaat atcataagtt actttataac tagcagttgg
    10561 aatggtttca atttctttta atccaataaa cgaagcagca gttaattttc gaacacctga
    10621 aacacttcct ggttcttgaa taaagttttg tgcataaaac tcttttttta atgtttcttg
    10681 acgttgttgt tcttgttcat gattatcgaa tgtattcata tacaaagcaa caaaacgatt
    10741 cataaaggat tcaatttcag gtgtgtacgt taattctgac cgactttgta cttctttctt
    10801 ttgtaattgt tggatttcat ctttcaaaaa cgacacttga accttcgttt tcatggattg
    10861 aaaaaatgcc aagccccctg aaaaacaaag aaataaaacc aaacaccaaa tccatttcac
    10921 tgcttttttt gaatggattt tcttttgctt cggtactttc tctgtataaa ctttttcttt
    10981 ccgccgtagt tgtcgttttt tcataagcta ctccttctag tatgttttta ctagtacgta
    11041 cgattgacaa cgataagctt gtttttctga aaaatagtcc attcaattac cctcattttc
    11101 tattcctttt ctctttcttt tatatccaac aaatccagca atacttgtca acactcctcc
    11161 tagaacagaa agtgcaacat gtatttcttc accagtcttt ggtaagactt ttgtttttcc
    11221 atctgctgtt ttgatttcaa tattgccact tggaagtttc tttacaggaa tcgacaaatc
    11281 ctttaccaaa ttcccttcac tatcttggat taatggaata ccatctttta caccaacaat
    11341 tgtttgatca cctgctactt ttaatggggt ggttggtgtt ggaatatggg tcaattcctc
    11401 tacattacta gtgacaatcg gtacactcat tccttgagaa gtattcgtcg taatttcacc
    11461 gcttggggtt accgatactg tgacttcttt tgctgaaaca ataggtttat tttcttttgg
    11521 aattgcttct gatttttcaa caggttgttt cggttcttct ttttgttctt cactggtaga
    11581 cgacatcgtt ggctgttccg tacttgacga atcagtattc ttttcagaag aactggttgt
    11641 tcctgattgt tccggagttg tgactatact tgaatccgtt gttgttgaac tatgcacacc
    11701 ttcttcttcg atcgaatcag tcggtaaagt tgattcagga aactctggtt ctggtgtcac
    11761 tggctctttt tcaggtttct ctgactgcaa ttgttcaggt tctgctggtt tttccgttgt
    11821 tcctccattt agctctgtct caacatctgg taatccttcc cctggcaatg tttctacaat
    11881 ttctgactta ggtgtttcta cagtttcatc tgcataaacc aatgaactcc ctaattgaaa
    11941 cgaaaataat gaaattccta gtgttgttac gactgctaat gttgtttttt tcatgattat
    12001 tcctccaatt ttttatgcaa ttcgtgtttt gttcatagac gccgcactaa tattatctga
    12061 acgataaaag aactcccgtc cgttttcttc taacccaaca tatggattat ttttgaataa
    12121 ttgaatcaat agttcactgg tgttgtattt tggatagttt gcaaaaaaat aatactgtgc
    12181 ttgtcgccca tccgaaaatt caacaagtaa ttcataacga taccgcttca atccttttcg
    12241 ttgttttaaa ggatctctat accaacttgt ccatcgttcc attcgtggat agagatagac
    12301 acgatacaca tagaacaatg ccataccaca taccaccaaa aataataatc gccacataaa
    12361 aattccccct tttcttctac ctttcttgaa tcatcgaaca ttcataccct ctatagagat
    12421 ttgaaaaatc catcatcaat tgttcatata aacgttgttg caaatagcga ttgtttcgaa
    12481 tacataattc ttcatagcca tcaatcactt gatcttcttt ttcatagcat cttgtagctc
    12541 tatgacgctc agcttgcaag acataaccaa actctgcacc atatcgttct actgttaata
    12601 actgttgact acgcaataat gcaatataac aatcacaaaa atgttcaacc gacatttgat
    12661 ttttctcacg tttgtgaaat aggtattctt tagaaagcac attattcgtt aaacaatctg
    12721 ataaatagcg ataatctggc ggtcggttat tttcatctgt gaatttttcc cataataaac
    12781 tgatttttat tcgttcagcg gctagatagt cttgttcgcg tggataaaat tcatcaatcg
    12841 cacaaagttt tttatcacct gaataaacaa atccatataa tgcgaaacga actcttcctt
    12901 ttcaattggc tctcctaaaa attccagtga cataccatct tctccttcct aaaaaaaata
    12961 aaaaagcaga aaccgatttg gcttctactt ttctttcctt ttatagctct tttttacgga
    13021 agaatccgca aatgacattt tttcaaaaaa tcaatcggca caagttcacg aaaacttgga
    13081 gcaattttgg atagaatcac ttccccatct tgtaaaatac aatcataaaa atcatcagtt
    13141 acttgatact tacgcttctt acaataattg ataaaatagt agcgatcatt ttctatttcc
    13201 attcgttgtt ctactaagcg catacgaaca tcatcataga aataagccac aaatcgccct
    13261 tcaagttcat gtttttcaat gggatactct tctggtaaca atctattttt tcgtcgttta
    13321 tacattgctg gataccttag aagaagttca tctaaacgtc tgtcagtata tggtaactga
    13381 cgaatcaacg attctaatcg ttctttatac agatagaata attggcgcaa gcaatagtgt
    13441 accgcctctg gattttctaa tactgttgga tcacgtgacc aatacgcacc aacataaatc
    13501 ataatttttt tcatttagac aatcacacca gcaatactaa tgaccgatcc acaataggta
    13561 gaacaagtgg catagcgacc aatttcctct aattctatct caatctcttc taaagaaaga
    13621 tatgtctgtt cttctgttgg ctggcgactc atttcccaag tgacagtcac caatgaaatt
    13681 tcttccataa gaggtttttc gctgctaagt gctgccacac agttttcata tatcttcatt
    13741 ttatcgcagc cttttttaac cgctaaaatc gcaggtattg gcaatgaaaa tcgataaaag
    13801 cgaatgaatt ctagccttcc tttccttcgg ctaaattcca actcttcgag ttcttgttcg
    13861 tatcccttac gaacttctaa taagcgtgca atttcttgcc agttctcttt ttcctgataa
    13921 cgtccagctt ttaccgcttg ttcaatataa ggaactcgaa aacgaaaata agacaaatca
    13981 accatctttt catttaatgc ttcgattgca gcctttaaat ccaacaggcg cagccattct
    14041 gttgtcgtat atgcattcat gcaattttcc ctcctgacgt tcccatccat tctagatagt
    14101 tttgttgctc gtcatttagt tcggcattat caatcccttc ttgtacaaag tctcttccta
    14161 aaatacgatc tgcttcttgt aacaacttta atgtcggagc tacttgtttc aaaaaccagc
    14221 gttctgaacg atctaatgag aatttttctg gctttactgt aaaatccaat tcttccatcg
    14281 aacgaaataa ttttgcccat cgtttatcaa caatccgtgc atcactttcc agtctatccc
    14341 aaacaataag ttttgcatta attaaaccaa cgccaatctc tcctaaacgt ttcggtgaat
    14401 cacctaaaat aaatgcttct actgcttgtg ttgctttctc attggctaac cgtaactcat
    14461 atcgattata aataccatat ttcgttaatg cttctaattc tgaaattttt tctcggttcg
    14521 caatttcaaa acgtttctca taaaaattaa aaaagagagg actctttcgc gatccaaaat
    14581 acaaagataa tccttcactt cgaaaatagc catcatctaa aaaacaaccg ccaatattct
    14641 ggaatctctt aaagtccatt tctaataaac ctgctttttg tttttctagt aattcaaaga
    14701 gatcgaaatg ttcttccgtt tccaatcccc aaagttcatc taacgcaata tcgaatcggc
    14761 tacattgcac acgtgtataa ccagcttcca actttccttg atttttccaa atggaatata
    14821 gccatgttcg ccaatctgtt tgttgttcac gcatgacttc ctcatattcc cggcaacctt
    14881 gtcctgaaaa ttgcacaagt acattttctg ttacagttcg ttttgaatgc cagaacaccc
    14941 agatattccc acgaacagaa agacagtcaa aaagatacaa gcccgttgga cgtgtttcaa
    15001 attctgtcag atccattcgt aatacacgtt gaatcactgt tttgtagtct gtcccaaaaa
    15061 aggaaagacg caaataatca atcattgctc gaagatctat tccgcttttc atatacaatc
    15121 gaaagcgttc cttgattcgt tgttcaaaat cctctgggat ttttcttttc ccttgttcca
    15181 tctttctaat gtaatctttt gagcagccca tcaattgcgc aaatcttgct tgtgatagcg
    15241 gctttttatc tccacttata ttatgaattg cacttaactt ctttctgtag ctttttagct
    15301 caatcggtgt cattttctcg ctccttttat aaaataacaa aatcgatttt tttaaccctt
    15361 tagaaacacc gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct
    15421 gaccttagat aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt
    15481 atttctgccc cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc
    15541 acgacctctt tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg
    15601 cagccttaat gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc
    15661 gatagataat aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg
    15721 agaaaaaacg cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata
    15781 caatcgagtc aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc
    15841 tgtaaaaaca cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa
    15901 ccaatcataa aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac
    15961 tctataataa caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg
    16021 catcagaaaa acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt
    16081 cttgtaacaa ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa
    16141 aatcatagac aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt
    16201 aaacaaatgg cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac
    16261 caggttgatc tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat
    16321 aaccaatggg agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac
    16381 ttggaaaata ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac
    16441 gaccctttaa tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca
    16501 acgaaccata aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat
    16561 aagcataatt tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc
    16621 gttgattcat ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt
    16681 aaaacacatg tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc
    16741 catcgcaaat atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat
    16801 aggttttccc accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc
    16861 aatccaaagt tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa
    16921 tccgaccaga atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa
    16981 ttaaatcacg caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa
    17041 aacgcccacc atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa
    17101 cttttggtaa tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa
    17161 caaaccgatt gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat
    17221 atccttcttt tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca
    17281 aatacaacac aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta
    17341 aacgtggata aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa
    17401 agaaaccata atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat
    17461 acttgaaaaa atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg
    17521 ctaaaaactt ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata
    17581 atcgaaaaag atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta
    17641 tacgaatcca aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa
    17701 caagatcacg taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta
    17761 caaaaatcgt tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag
    17821 cacaatctct caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta
    17881 tcttagataa aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc
    17941 tctttttcct tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt
    18001 ttcatattag gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact
    18061 aaatctgaat attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga
    18121 tcaattaatt tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat
    18181 ttaactcgtc gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt
    18241 gatcgaattt ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct
    18301 aatgcgccaa agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa
    18361 ttatttggaa attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa
    18421 gacaccaaca tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc
    18481 acgactattc actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac
    18541 ctctttttct ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc
    18601 aggctgttct tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt
    18661 aatttccttt ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc
    18721 tttcttcata cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat
    18781 aaattctaaa tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt
    18841 cctcatgact aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact
    18901 aatactacta atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac
    18961 attggtacaa cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt
    19021 ggcgcttcca caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc
    19081 acttcttcaa acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc
    19141 aagccttttc ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt
    19201 tctttatcgg ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt
    19261 gttaagtagc cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg
    19321 acccattcaa gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc
    19381 gtcgctttcg tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata
    19441 tctgcgtgag aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct
    19501 ttgcctcgca acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt
    19561 tctgccacaa acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc
    19621 gttactttgt ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg
    19681 tatttcacag cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg
    19741 ggtttatgtg tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg
    19801 tcgttgatgt ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg
    19861 acgatttcac gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca
    19921 ttttctgctt tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag
    19981 ggttcaccgg ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga
    20041 atctcggtaa tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc
    20101 aatccgtctt caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg
    20161 gcttcatcgt tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca
    20221 aaaacaacga tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt
    20281 gtgccattga ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt
    20341 aaaattggcg cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg
    20401 acaaacaagt tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc
    20461 actttttcac catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa
    20521 ttacgtacag aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc
    20581 gtgaatgctt gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag
    20641 taatagtcat cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat
    20701 tcaccaattg cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt
    20761 ttcttcttgt tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc
    20821 gcaaaatgga tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt
    20881 tgattcaaaa cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca
    20941 taagtaagtg taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt
    21001 gtttctgtat catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc
    21061 ttgaattctg tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact
    21121 ttaagacgtt gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca
    21181 gaaattgtga cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa
    21241 agaatgtacg tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct
    21301 gtcacaacgg ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca
    21361 gctaattttg gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc
    21421 actaaatcaa tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca
    21481 ggttcaacaa caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt
    21541 tcttttaaaa catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta
    21601 atttttttca aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc
    21661 gcattttcta aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac
    21721 acacgaatgt ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt
    21781 ggatgtaaca caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga
    21841 actttaattt tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc
    21901 catacttcat tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca
    21961 tatttcaaaa aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt
    22021 gtttgattat taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct
    22081 tttcgacgat cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc
    22141 caaatcataa attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat
    22201 cctaaataag agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc
    22261 aaagcagtag ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac
    22321 gccacttgtc cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc
    22381 ggactagaag taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct
    22441 gcattggttg cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta
    22501 ggtgtacctg tttttggaat gttctgctct tttttctttt gattttcaat actagccgta
    22561 gattctttgt ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt
    22621 tcaacgtttt cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt
    22681 tcttctattc gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc
    22741 gttgcttcgg cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt
    22801 gaggcagttt cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc
    22861 ccaatcgtct gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc
    22921 tgttcaacag tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact
    22981 tcagaagcga cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca
    23041 tgtgcttgag cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct
    23101 ttcaaccaat tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt
    23161 atataataaa acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt
    23221 tttatctggc gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt
    23281 acagaacaaa aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac
    23341 cagctttttt ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat
    23401 ccttaatttc tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac
    23461 tattgccaat tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac
    23521 cataatgatc cgattcctca gtattattgt cattactagc tactggtttg gttgttctac
    23581 tggcttattt tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc
    23641 ccattcaaac cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa
    23701 ataggcatct tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata
    23761 gacacctacc catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt
    23821 tctaagaatc tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat
    23881 tctatcacct caaataactc tagattctca taaatattcc cgacaatttc aatcctatca
    23941 atgatctcta acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca
    24001 aaataaattt tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt
    24061 ccacgttgtt cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct
    24121 cgaaacaatc acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt
    24181 gtcctcgtgt attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc
    24241 gaaatctagg aatcacaact tattcgcttc ttctttataa ataaaaaaac tccaataatc
    24301 ggaaattact atcttttcct cattcctttt ttaaatcttc atacttcttc ttatctttgt
    24361 cctaacctaa taacacaaat tttgaagaaa aactcagtaa aacctgattc tttttttatc
    24421 ttcacaacaa ccaacttttt cttcagatcc ttttactaga acttgtatac aaaaattgtt
    24481 gaatgctgat ataatttgcg gctacttgaa aaaacgatag atgtaaaaag ataaatag
//
LOCUS       DAZAJZ010000041        24427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000041 DAZAJZ010000000
VERSION     DAZAJZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.041"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG86D_001531"
     CDS             complement(54..788)
                     /locus_tag="ACG86D_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN3140674.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG86D_001532"
     CDS             1115..1525
                     /locus_tag="ACG86D_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN3140675.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG86D_001533"
     CDS             1518..2219
                     /locus_tag="ACG86D_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN3140676.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG86D_001534"
     CDS             2219..3805
                     /locus_tag="ACG86D_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140677.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG86D_001535"
     CDS             3824..4045
                     /locus_tag="ACG86D_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN3140678.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG86D_001536"
     CDS             4042..4803
                     /locus_tag="ACG86D_001536"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN3140679.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG86D_001537"
     CDS             5183..5692
                     /locus_tag="ACG86D_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN3140680.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG86D_001538"
     CDS             complement(5767..6306)
                     /locus_tag="ACG86D_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN3140681.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG86D_001539"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG86D_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN3140682.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG86D_001540"
     CDS             7319..8170
                     /locus_tag="ACG86D_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN3140683.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG86D_001541"
     CDS             8198..8833
                     /locus_tag="ACG86D_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN3140684.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG86D_001542"
     CDS             8853..9626
                     /locus_tag="ACG86D_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3140685.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG86D_001543"
     CDS             10034..10831
                     /locus_tag="ACG86D_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN3140686.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG86D_001544"
     CDS             10809..11222
                     /locus_tag="ACG86D_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3140687.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG86D_001545"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG86D_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN3140688.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG86D_001546"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG86D_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN3140689.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG86D_001547"
     CDS             15999..16559
                     /locus_tag="ACG86D_001547"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN3140690.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG86D_001548"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG86D_001548"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN3140691.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG86D_001549"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG86D_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN3140692.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG86D_001550"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG86D_001550"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN3140693.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG86D_001551"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG86D_001551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN3140694.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG86D_001552"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG86D_001552"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN3140695.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG86D_001553"
     CDS             21906..22484
                     /locus_tag="ACG86D_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3140696.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG86D_001554"
     CDS             22536..23018
                     /locus_tag="ACG86D_001554"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN3140697.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG86D_001555"
     CDS             23135..23509
                     /locus_tag="ACG86D_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140698.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23939..>24427
                     /locus_tag="ACG86D_001556"
     CDS             23939..>24427
                     /locus_tag="ACG86D_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN3140699.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         8007 a         4390 c         5213 g         6817 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggta ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
    23941 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
    24001 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
    24061 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
    24121 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
    24181 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
    24241 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
    24301 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
    24361 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
    24421 tatggac
//
LOCUS       DAZAJZ010000042        24260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000042 DAZAJZ010000000
VERSION     DAZAJZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.042"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG86D_001557"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG86D_001557"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN3140700.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG86D_001558"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG86D_001558"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN3140701.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG86D_001559"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG86D_001559"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN3140702.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACG86D_001560"
     CDS             complement(3373..4680)
                     /locus_tag="ACG86D_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN3140703.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACG86D_001561"
     CDS             complement(4683..5264)
                     /locus_tag="ACG86D_001561"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN3140704.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACG86D_001562"
     CDS             complement(5836..7128)
                     /locus_tag="ACG86D_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3140705.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACG86D_001563"
     CDS             complement(7125..7748)
                     /locus_tag="ACG86D_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140706.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACG86D_001564"
     CDS             complement(7751..8587)
                     /locus_tag="ACG86D_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140707.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACG86D_001565"
     CDS             complement(8604..9491)
                     /locus_tag="ACG86D_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140708.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACG86D_001566"
     CDS             complement(9507..10790)
                     /locus_tag="ACG86D_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140709.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACG86D_001567"
     CDS             complement(11473..12156)
                     /locus_tag="ACG86D_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN3140710.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACG86D_001568"
     CDS             complement(12174..12791)
                     /locus_tag="ACG86D_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3140711.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACG86D_001569"
     CDS             complement(12801..13625)
                     /locus_tag="ACG86D_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN3140712.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACG86D_001570"
     CDS             complement(13618..14178)
                     /locus_tag="ACG86D_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140713.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACG86D_001571"
     CDS             14352..15308
                     /locus_tag="ACG86D_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3140714.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACG86D_001572"
     CDS             complement(15613..16569)
                     /locus_tag="ACG86D_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN3140715.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACG86D_001573"
     CDS             16673..17275
                     /locus_tag="ACG86D_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3140716.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACG86D_001574"
     CDS             complement(17363..18862)
                     /locus_tag="ACG86D_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN3140717.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACG86D_001575"
     CDS             complement(18878..19402)
                     /locus_tag="ACG86D_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN3140718.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACG86D_001576"
     CDS             19700..20449
                     /locus_tag="ACG86D_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3140719.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACG86D_001577"
     CDS             20440..21225
                     /locus_tag="ACG86D_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3140720.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACG86D_001578"
     CDS             21194..21910
                     /locus_tag="ACG86D_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3140721.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACG86D_001579"
     CDS             complement(22012..23031)
                     /locus_tag="ACG86D_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN3140722.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACG86D_001580"
     CDS             complement(23050..24051)
                     /locus_tag="ACG86D_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN3140723.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5221 c         4225 g         7626 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DAZAJZ010000043        23551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000043 DAZAJZ010000000
VERSION     DAZAJZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.043"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..926
                     /gene="proB"
                     /locus_tag="ACG86D_001581"
     CDS             120..926
                     /gene="proB"
                     /locus_tag="ACG86D_001581"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN3140724.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            923..2170
                     /locus_tag="ACG86D_001582"
     CDS             923..2170
                     /locus_tag="ACG86D_001582"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN3140725.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2246..2590
                     /locus_tag="ACG86D_001583"
     CDS             2246..2590
                     /locus_tag="ACG86D_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140726.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2707..3033)
                     /locus_tag="ACG86D_001584"
     CDS             complement(2707..3033)
                     /locus_tag="ACG86D_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140727.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3648..4850)
                     /locus_tag="ACG86D_001585"
     CDS             complement(3648..4850)
                     /locus_tag="ACG86D_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3140728.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4967..5482
                     /locus_tag="ACG86D_001586"
     CDS             4967..5482
                     /locus_tag="ACG86D_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3140729.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5681..6127)
                     /locus_tag="ACG86D_001587"
     CDS             complement(5681..6127)
                     /locus_tag="ACG86D_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN3140730.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6174..7652)
                     /gene="msr(C)"
                     /locus_tag="ACG86D_001588"
     CDS             complement(6174..7652)
                     /gene="msr(C)"
                     /locus_tag="ACG86D_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN3140731.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8006..8635)
                     /locus_tag="ACG86D_001589"
     CDS             complement(8006..8635)
                     /locus_tag="ACG86D_001589"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3140732.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8619..9083)
                     /locus_tag="ACG86D_001590"
     CDS             complement(8619..9083)
                     /locus_tag="ACG86D_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3140733.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9570..10838)
                     /locus_tag="ACG86D_001591"
     CDS             complement(9570..10838)
                     /locus_tag="ACG86D_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3140734.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11069..11806)
                     /locus_tag="ACG86D_001592"
     CDS             complement(11069..11806)
                     /locus_tag="ACG86D_001592"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3140735.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11873..12442)
                     /locus_tag="ACG86D_001593"
     CDS             complement(11873..12442)
                     /locus_tag="ACG86D_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN3140736.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12570..13442
                     /locus_tag="ACG86D_001594"
     CDS             12570..13442
                     /locus_tag="ACG86D_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3140737.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13784..14656
                     /locus_tag="ACG86D_001595"
     CDS             13784..14656
                     /locus_tag="ACG86D_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3140738.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14653..16611
                     /gene="nagE"
                     /locus_tag="ACG86D_001596"
     CDS             14653..16611
                     /gene="nagE"
                     /locus_tag="ACG86D_001596"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN3140739.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16943..17794)
                     /locus_tag="ACG86D_001597"
     CDS             complement(16943..17794)
                     /locus_tag="ACG86D_001597"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3140740.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17917..18384
                     /locus_tag="ACG86D_001598"
     CDS             17917..18384
                     /locus_tag="ACG86D_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN3140741.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18647..19129)
                     /locus_tag="ACG86D_001599"
     CDS             complement(18647..19129)
                     /locus_tag="ACG86D_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3140742.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19229..20092)
                     /locus_tag="ACG86D_001600"
     CDS             complement(19229..20092)
                     /locus_tag="ACG86D_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3140743.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20690..21565
                     /locus_tag="ACG86D_001601"
     CDS             20690..21565
                     /locus_tag="ACG86D_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN3140744.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21710..21862)
                     /locus_tag="ACG86D_001602"
                     /pseudo
     CDS             complement(21710..21862)
                     /locus_tag="ACG86D_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(22130..23461)
                     /locus_tag="ACG86D_001603"
     CDS             complement(22130..23461)
                     /locus_tag="ACG86D_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3140745.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         7243 a         4763 c         4183 g         7362 t
ORIGIN      
        1 tcgttctttt tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac
       61 tcgtcaaaac aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca
      121 tgcgtaaaga aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga
      181 tttatcccaa tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg
      241 atcttagaaa tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta
      301 tgcataaatt acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg
      361 ctgttggtca agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac
      421 agacggcaca gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg
      481 tgaggaatac attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg
      541 atactgtagc agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt
      601 cagccatcat ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg
      661 ggttttattc agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata
      721 aaatcgatga aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg
      781 gtatgcagag taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt
      841 tagcaaatgg aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta
      901 ctttatttaa ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga
      961 tagcgcgcaa caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat
     1021 ggcacaaacc atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga
     1081 aaaagcagtc gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga
     1141 gcgaatcaat gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg
     1201 aaaagtagat gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc
     1261 actaggcgtg atcggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag
     1321 tctagcattc aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc
     1381 gaatcaatta ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta
     1441 tgccatccag tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac
     1501 agaatactta gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga
     1561 acaggcaacg gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa
     1621 agaagcacag ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc
     1681 tgtctgtaac gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc
     1741 agctattgaa aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt
     1801 agaatatcta gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga
     1861 ttatatcctt gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg
     1921 gcataatact aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt
     1981 cttaaatgaa gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg
     2041 atcggtattt ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg
     2101 tccaatgggg ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat
     2161 ccgcgaataa tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt
     2221 tctatccaac tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct
     2281 tatttctctg aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt
     2341 caaaaaacga agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg
     2401 gcaaaaaggg ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc
     2461 gacaatcaat tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat
     2521 tgctgggatt tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac
     2581 tttccgctaa aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc
     2641 cggacttttt agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc
     2701 gttcgcctac cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc
     2761 aaatgtttcg ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc
     2821 tttttcttct tccgctgatt cagccagtag ctgttcccga tacacctgat acataccagc
     2881 tattttttgc tgcatcgctt ttctagtttc accttccgca ttgattattg gcaattcttt
     2941 gatatagcca cgataaatca accgatcatc tgaaaattca atgggtaatt caacgagtgt
     3001 caacccgact tcctgaaaat attcttccag catctattat ccccccattc tttttcttaa
     3061 atgaatttta gcttacctac tgaaaatttt ccagtttcat tattttgaga aaaaaaggga
     3121 atgtgacaaa aatcgtgtca catccccttt ttccgaataa atggtgttct tctgccaact
     3181 cctcaatcat aagtcacaat attcaacaaa catgagaaac tgttttttga aattgttcct
     3241 tgtgattcag agttaaacga cagtctcaca acctcttggt aaacggcgtt caatcagctg
     3301 accctcggta ttagctcaaa aaacggtaaa atatgagaag ctatttttcc gttttgtttc
     3361 tctaatactt aggataaaac gctgatttta caacctctgt taaacggtgt tcaatcagct
     3421 gtccttcggt attagctcaa aaaacggcaa aatatgagga actatttttc cgtttgtttc
     3481 tctaatactc aggacaaaac gctgatttta caacctcctg ataaacggtg ttcaaaagct
     3541 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
     3601 gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca
     3661 tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag aacaatggca
     3721 cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat
     3781 ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt
     3841 tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa
     3901 aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat
     3961 aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac
     4021 ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca
     4081 aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca
     4141 agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta
     4201 tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt
     4261 tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa
     4321 aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt
     4381 ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata
     4441 ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca
     4501 cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg
     4561 atgataaaca atcctgtcat caatgttttt ttcaaaccga tccgactaga taagaaacct
     4621 gtaaatagca tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca
     4681 gctggcgtag cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact
     4741 cccataaaag cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt
     4801 tcttcatttg tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct
     4861 ctattagcat catacatgat ttattttaca tgtcaatatg cacatgtaaa acgtggaaac
     4921 tttactgtga tataataaag aaaataagac gagaggtagg atttttatga atactttagc
     4981 ctatatatta ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct
     5041 gatcacaatt ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga
     5101 ccagcagggt tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca
     5161 cttgactgat gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc
     5221 tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac
     5281 agctactgga ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca
     5341 aaaaaaattg aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat
     5401 ccagcgcaga attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca
     5461 aatgcagcag ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga
     5521 gcaaattatc ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca
     5581 ggccaaaacc tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca
     5641 aaaagcatgc caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg
     5701 cttttagcac acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa
     5761 tcacattcat tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt
     5821 ccatgatcac accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa
     5881 tagttaagat catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt
     5941 tcgccagcac accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta
     6001 gaccaatcaa caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca
     6061 tccccaagac catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg
     6121 taggcaatct gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa
     6181 tttctcgtaa gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta
     6241 cgctcgacaa agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt
     6301 agcttctcta aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc
     6361 aacacattac ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca
     6421 cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa
     6481 ctacggacaa gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct
     6541 tcagatgtca tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga
     6601 aggattcctt ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt
     6661 tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat
     6721 gttctcccac ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt
     6781 tcaagagatt ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct
     6841 cctatttttt ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca
     6901 ctgtcttttt gtttagagga agacaaccga tccagtctga tttgttgctt tttcttttta
     6961 ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat
     7021 cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc
     7081 ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact
     7141 tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc
     7201 gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg
     7261 gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg
     7321 atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg
     7381 aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc
     7441 aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct
     7501 attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct
     7561 tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt
     7621 agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa
     7681 caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca
     7741 tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca
     7801 cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac
     7861 atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca
     7921 gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag
     7981 ctactgaata gtaactgaaa accttttatt cgaacatagt gatcttttct ggtgctaaac
     8041 gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat
     8101 aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg
     8161 gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag
     8221 aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga
     8281 tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt
     8341 tgtagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag
     8401 aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt
     8461 cctcgctttc agcgaccaca aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg
     8521 tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata
     8581 cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta
     8641 ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt
     8701 gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct
     8761 ttatggtcca tttcacaatg cttgattttc ttctgggctt tttctgttag cgctacatac
     8821 acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc
     8881 ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt
     8941 agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg
     9001 atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg
     9061 tgactaatct cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt
     9121 gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa
     9181 aaacgctctc aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa
     9241 tgataaagaa agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat
     9301 agctcttctg cagtagataa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag
     9361 aatcccgtcg aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga
     9421 caagatgttg tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa
     9481 taagttcaac caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg
     9541 atgcagggca gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat
     9601 ctccagccca tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta
     9661 ccagtttttt catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa
     9721 cttagacctt cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt
     9781 gtgtaatgat ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt
     9841 ggtgcttgcc aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct
     9901 ccggcattca atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga
     9961 gaagttgaag ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc
    10021 aatgtagaac cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct
    10081 ttgtaagagt tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa
    10141 gaagtcgtct tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt
    10201 ctaaaagcgg cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta
    10261 acagattggc cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc
    10321 aaagtactgt tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat
    10381 aagtcattgg cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt
    10441 tgagaattag cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac
    10501 aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct
    10561 tctgcattct ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt
    10621 tctgttgttt cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac
    10681 tgcaaagtac tttctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt
    10741 gcgacagttt ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct
    10801 aaaagaataa cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg
    10861 aaacttttcc taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta
    10921 aataacattt gtttcttttt aataaatttg ctttttctgc ataaaaagac gtaaatgaag
    10981 aatattcttc tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta
    11041 attctctctg gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca
    11101 tgttgctgac agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat
    11161 cttcagatgt taatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt
    11221 cgctgatggt ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga
    11281 tgttattttg ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg
    11341 ttccgcaata aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa
    11401 tgatatgccc agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca
    11461 cacccatgat gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg
    11521 gagcggttgg catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca
    11581 tcgctaaatc gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat
    11641 atagctcagc ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag
    11701 ctataactag tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc
    11761 ttgaagcgcc catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct
    11821 gttttgaaaa atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa
    11881 tccgttcgac aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga
    11941 catctttcgt aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg
    12001 gcagaatagt tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg
    12061 acaagcctct tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag
    12121 gaccaatcaa tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac
    12181 gattgataaa gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca
    12241 gcagaacagt caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga
    12301 tatctgctgg ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttccgctt
    12361 ggatctgatg gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt
    12421 gtttttcgat gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg
    12481 cttatgatgt ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg
    12541 tatgcttttt taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag
    12601 cagtagctag agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta
    12661 ctctctcaaa acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcgag
    12721 gctcccgaga gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa
    12781 tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag
    12841 aaatctacat tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt
    12901 cgttgatcaa ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata
    12961 tcatagacaa actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca
    13021 aacaaaaata ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc
    13081 ggaatcatcc tttagcaaag caaacagtca tccatccgca ggaactgaaa actgttcctt
    13141 tgatcatttc ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca
    13201 tagatcagtt atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg
    13261 ataacgggac ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa
    13321 aagtattcat cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag
    13381 accagcctct ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt
    13441 aagtagagtt tttttccttc gctttcagct ttttaacaac gaataagtta agcaaaagat
    13501 ccaatgatta aataacataa gtttcttaat ttgttccttc cttttatttc tgataattcc
    13561 agtaaattac tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg
    13621 attacgttaa caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg
    13681 aggaacatgg tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg
    13741 gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat
    13801 ctttaatcaa aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa
    13861 agggattgga tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat
    13921 atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga
    13981 acgcttttta tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa
    14041 gttgaatgaa catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat
    14101 taataacggt atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga
    14161 tgaattcagt attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata
    14221 tacttatgac gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa
    14281 caataaccat cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga
    14341 atcagaattg gaaacagata tccattcaga accattttca ctgaattatt caagactggt
    14401 gaatcattta cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac
    14461 ggaaatcgta gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat
    14521 ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt
    14581 ggccatccat atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga
    14641 ggaggaaaaa aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt
    14701 gcggtcttac ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt
    14761 aacgacattg tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct
    14821 ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc
    14881 ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct
    14941 actttattga atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta
    15001 tttattggta ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca
    15061 aaattaccga tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc
    15121 tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct
    15181 ttagtttctt ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc
    15241 ttcttcaacc gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg
    15301 tttgacgtag caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc
    15361 gtaacaggaa tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga
    15421 gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg
    15481 ttagcagcag cttttgcttc attctttact ggtgtaacag aaccacttga attctcattt
    15541 atgttcgttg cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca
    15601 gtgagtgcat tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac
    15661 ttcctatctt tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta
    15721 gtaacagcag tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg
    15781 actcctggcc gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac
    15841 aataaatatg cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta
    15901 acttcgatcg ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc
    15961 gatcaaaata aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat
    16021 gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat
    16081 ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc
    16141 ggtggaacga tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact
    16201 gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca
    16261 gaaaatggag aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa
    16321 catgctgtcg gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac
    16381 actgttgact taaaaggcga accattcact ttatatgtaa aagaaggtca aaaagtcgca
    16441 agaggtcaat tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg
    16501 gatatggtcg ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca
    16561 cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc
    16621 ttccccagac ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct
    16681 cctttttccg aataaaaacg gcgttctcct gccaactccg cggtaataag tccagccaaa
    16741 cgtaacatat gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct
    16801 tttttacaac ctctgataaa cggtgttcaa aaaccagttt atcggtatga agtcaaattt
    16861 atgcaaaaat gtggaaagcc attttcacaa aatttttctt catactcaaa actaatcggc
    16921 tttttcacaa cctctttata cattattcct gatccaatat ttcttttaat gctgcttcaa
    16981 gagaagttaa tggttttccc aatacttttt ctaaatcatc agaggaaata gctaactgat
    17041 cctttttaat atcatgttgg atcgctaaaa acatgtcagc tgcttcttga gggaatccgg
    17101 cttcttttag attttcgaca aacgctgcat cacttgcttc aataatttct aactttttac
    17161 ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc agttagattg ccggataatt
    17221 ctaagatttc tggagagtca ctttctacta gtacttttgc tgcagcttct gcatattcac
    17281 gtttcaatgc ccaacctgtt tttccatttc ctgcagcata aacgaattga ccagttttta
    17341 aagcctgccc aatgataggt aattcattct ctacatacca attattccgt aaaaatgtat
    17401 gggaaatacc tgaatcctta atcatttttt cagtgaattg gtgatctggc gctaatacac
    17461 tgatcgagtg atcggcatct gcaaaacttg tataagcgat ataagatacc ccagctgctt
    17521 gtgctgcctt cactacattc ttatgttcgg cttgacgatt tcctggaacg ccagagacaa
    17581 aaagcaatcg atctattcct ttcaacgctt ctttcattga gtctaaatca tcataatcac
    17641 caatacgtat attaaatcca gcttctttca gtgcttctcc tttttcttca ctacgtacta
    17701 gtacatagat ttcattttgt ggaaccatct tttttaagaa ttccagcgca taaccaccaa
    17761 aacctccagt tgctcctgtt actaaataat gcatatttgc cactccctat tctttttatt
    17821 tgttaagatt tacagctgta catttataaa ttacaccttt atcataaaga gtgtggtaag
    17881 ataagtcaag caaaaaaaat gaaaggatga caactgatga agtattctct gcaatttagt
    17941 gatgcaatcc atgtacttgc ctatattgaa atgtttcaag atacccgatc actttctagt
    18001 gagatgattg ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag
    18061 ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac cgcagccttc ccttgccaaa
    18121 agtccagatc agatttcttt actagatgtc tatcgcagca tcgaaggtaa tacacagctt
    18181 atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct
    18241 ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag aagaaatggc taagatcaca
    18301 cttgatagtc tgatacataa aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca
    18361 ggcctcgtag agagattttt ataattctct ctactgtgcc tgcatttttc tgatcggaat
    18421 cgtctacata aattctttta ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct
    18481 ctctaatgct caggtcaaag cgctgaagtc atgacgtctt gataaacggt gttcaagtag
    18541 ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga aagctatttc ccatttttcc
    18601 tctctaatgc tcaggtcaaa gcgctacttt cacgacctct atattatcat ggtttattga
    18661 ctaccggtat ccagatttct cccatatatt tcccgttggt aactcccatt tcgactacag
    18721 catttggacc cccaacatag gcgtaatctt ctacttctct taaaacatgc ccaaaggccg
    18781 tattcgtcaa atcatttctt aattcttcag gcgattctgc taaagcgatt acaacaagat
    18841 acgttccttt tgggaatggg atgattcggg tagtgtttgg aacatcctga ctcgtttgaa
    18901 caccgacata gtacatcatt ttcccgttat agggttcgtt gactgtaaag aaataatgat
    18961 tttcagcagc ttgtttcaat gcatctattg ttccattacc gataactttt tgctcaaatg
    19021 cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct actcttcaat tccacaccaa
    19081 tacccgtcac cgtaaaatct ggtttttctt cgatcctata ctttgccatt tctcccaccc
    19141 tttctattat attaagttca tcttacataa aattaaaggg aaatgacacg attacgctct
    19201 tttttctagg gatattttta ttttttaact atttttttgc tttcactgca atccaaatct
    19261 cactgtaaac attactgaaa tcctctcccc attttgtaaa agagatctcg ggagcttcga
    19321 ctaactcata atctgaagaa ggaagccatt ctgcatagat tttcccccaa gtatcttgta
    19381 aagtttgggg aaatggccct ttattaggaa aaatggccca agtgtgttct tctatagcaa
    19441 gcatttctaa atcagcgtac ggattttctt ttgtcgtagc aaagccaatc atgtgcatca
    19501 aactgccctt ttcttccaag cgggctccat caaatccata agaaacgttg attacttgat
    19561 ttggatataa atctcctaac ttatgcatct cttccctttg agtctccgtg atcgtctgcg
    19621 ctaacttttg tatttcttga ttgactcctt caaatttgac aggaactttt ttcgccacac
    19681 ctgccacata aaactgttct ttcttttcga tacgaaattc catcgatttt cctcctctga
    19741 catcaataaa aaatgtcaag cgtggaaaag atttttgtat gccgtatctg ctgatttctg
    19801 aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact gtttcatatc
    19861 catatctaaa cgccgtttct gtcactcttg atccatctag gagatcgcga ttagccaaag
    19921 ccaatctacg gtttttgata tagtccatta gcgacatccc tgcaataaac gtaaagatgc
    19981 gttttaattg atattcagag attcctgtat tttcggctat tattttatag gaaagtcctt
    20041 ctgtcaaatg ttcttcgatg taatcgatca attggttcag atctttaagc atatttatct
    20101 tcctccttgc ttgccttttt atcatattat caaagaaagt caattacttg atgaatcagg
    20161 aaccaattta gtcagatgta gaaaatgttg tatggatcta aagaaaattc tcaagcagcg
    20221 tttcgtaacg gataattggt attatctgcc tatctatgtt ttacctttag aaaaataaat
    20281 tttggggaag acacattcta agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt
    20341 aatccgctta ctacctactg tacaaagcaa attgttgttc aaactgctga tcagaacgct
    20401 gcttggattt ttctttgcga tacatcatac ctcttttatc aattggaaca attatttatt
    20461 tctaacaaaa tcacttcatt tttgtcagat aaataaaaaa caaaaaaagt tccatcttta
    20521 aaacacgcta ttcttttact ttttctcaca ttctctaaga aaagaaaagg agaatgaatt
    20581 tttgacaatt cgagcaaaag tgattatcaa atatgtaaaa tcagctactt ttttcttaaa
    20641 cgtttcttta tactaaatat aaataagtgt ttcatgaaag gagaactaga tgaataattg
    20701 gcttgagtat tttcctgaaa acgttttgga acgcggttac agctatcatc tgcacggttt
    20761 tgtccgtcat ttgaactata caagcaagta cttgtccgca acagtttctg gcacggaaga
    20821 ttataaggta gtgatcactt gggatgaaaa gaccaacatg acttgtgatt gtctatatgc
    20881 catagaagga aaaaaatgca aacacatggc tgctgtctta ttcgcttacg aagaacgtcc
    20941 aataaaaaaa tccaactact cactttccga actctcgtcc cttgtttcct ccgctagttc
    21001 ctctcttgta cgggagcttt taacagagat tcttatagaa catccgcaat ttatcgaacg
    21061 cttcaaagtt aagatgccat ttcatgcaat aaattattca gataaattaa caactatcat
    21121 ccataaatac gatcatatta tcaagaaaaa caaaaacagg aaaacagcga agttcatcat
    21181 ggagatgcga aagttcatcc aagaagcagt tgaatcgctc attcagcaaa atgcctatct
    21241 tcccgcattc gaattgatca atgaagtgat tgctactctc gaaacattct attgggagcc
    21301 tgaggatgaa cgaacccttc ttctgataga ggactgttat tatttatgga aagaattatt
    21361 agcagaagca cctcatgcag aaaaaaggca aatgttctct tggttcgtct gccaagtcga
    21421 tcatactgac gcctcttatt caaaacgcta ctccataaaa atcctgaaag aagactttcg
    21481 ggaaaaagaa ttttctaacc aaaagaaaaa aatagataaa aaaactaaag aaacggtaaa
    21541 gaaagacgat ttcaatgaaa aatagcaagt aaacgataac aaccatccta aatccacaag
    21601 aaatacagca gaccttgctt taaaaacacg aatcatgcaa aggatgaagt aaagaagact
    21661 taacgcgtac ccaatattag tcatttgact attaggtacg cgttttgttt tataagtcat
    21721 gacaaatttc cttcgttgct gggtagattt tccggcattg ttgatagatt ttatgatact
    21781 tttctacatt ttgtaagaga ggttccactg catctgtata ttggataaaa ttctgacaac
    21841 aactttcagg tgtgtcgaac cactctaatc tttaaagata aatattaaaa taacgattaa
    21901 taatattaac agtctttatt tttcaccacc aaaaacctat ctttgcatat actaaataga
    21961 cgtatataca cagataggtt tgtcttgtga aaaataatga ttatccttac caaaaaatgt
    22021 ttgtttttct acatgttcgt acttatcaaa aaatatgatc cttattgaaa ttactggcta
    22081 tttatattcc aacgacttct tgtgttaatt tttgttatct catagtaaat tatgtttcat
    22141 tttcacttaa tttagttgtc acgtgttcgt caattcgtaa aaacggtaaa tatataaaca
    22201 cacttgcggc tataattaat atttgtaaaa cagctgctcg ccagtctccg gctgttgcta
    22261 aaaatcccga tataactggt ggcgtagtcc aaggaatttg cactactaca tgattaatta
    22321 atcccacagc agttgcaaaa tatgctactg tcaaagaaaa aatcggtgca agcacaaagg
    22381 gaataatcag actgatatta aatacaatag gcaatccaaa aataataggt tcgcttatat
    22441 tgaatatagc gggagttacc gataatttag caaagtcctt ataatctttt ctacgactga
    22501 agataaatat tgcaatcaat aaagcaatcg tatttcccga tccacccatt accgcaaagg
    22561 tatctttaaa ggccatattg ataatatgtg gaatttgctc tttgtttgca taagctgcca
    22621 tattttcttg catattctga atcaaaaacg gatctaacaa tgaacctgat attactgctt
    22681 gatggatacc aaatccaaaa aatagattag caatagatgt aattaacaaa aatcctggta
    22741 aaccagttgt aattttactt aatggctctg taactatcgt ggtaattagc ttatttatat
    22801 ccatattaaa caattgaatg attataaatg acaataaagc aaacgaggta atcgtaataa
    22861 tgattgggat taatacatta aaagacttta aaacagcggg aggaatattt ccggatagct
    22921 gaatctgtag tttcttatta ttagaaagtt taataaataa ttctgtagcc gctaatccaa
    22981 caaatatacc tacaaacatt cctgaagctc ctgtatagga tataggtatc acacccgtca
    23041 cttcaacagc tttatccgca ttttcaggag taaacatcat ctgcataggt gttaatacaa
    23101 caaatgtcga aatagcaact aataccgctg caatatgatt tgtataattt ctgcttgcac
    23161 acatatgata agatactgct gctacaacta ataatgtagt tatgcttaat gtaccattgg
    23221 caattgaagt accaatttgt tgtaacgtgg tcaatgtact agtatcgaca atttccccca
    23281 tgaatcctgt tggttctaag atcactgaat taataagtgt cacaaatcct gctaacatca
    23341 taaatggcat aaatgtagca aacgcatctc gcaaagttct taaatggatc tgattcccca
    23401 acttagtcgc aactatacca aatttttcaa atacaatgtc cttcctgcta cttttgccca
    23461 tattttttct cctttatgct tattgtaatc gattactttt tccacccaaa taatacatca
    23521 cttatcaaaa aaagtaaata cttttaatta a
//
LOCUS       DAZAJZ010000044        22488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000044 DAZAJZ010000000
VERSION     DAZAJZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.044"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..198)
                     /locus_tag="ACG86D_001604"
     CDS             complement(1..198)
                     /locus_tag="ACG86D_001604"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN3140746.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(344..1222)
                     /locus_tag="ACG86D_001605"
     CDS             complement(344..1222)
                     /locus_tag="ACG86D_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3140747.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1261..2379)
                     /locus_tag="ACG86D_001606"
     CDS             complement(1261..2379)
                     /locus_tag="ACG86D_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN3140748.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2376..4475)
                     /locus_tag="ACG86D_001607"
     CDS             complement(2376..4475)
                     /locus_tag="ACG86D_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN3140749.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4526..5347)
                     /locus_tag="ACG86D_001608"
     CDS             complement(4526..5347)
                     /locus_tag="ACG86D_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3140750.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5344..6153)
                     /locus_tag="ACG86D_001609"
     CDS             complement(5344..6153)
                     /locus_tag="ACG86D_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3140751.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6174..6668)
                     /locus_tag="ACG86D_001610"
     CDS             complement(6174..6668)
                     /locus_tag="ACG86D_001610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3140752.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6680..7180)
                     /locus_tag="ACG86D_001611"
     CDS             complement(6680..7180)
                     /locus_tag="ACG86D_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3140753.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7245..9941)
                     /locus_tag="ACG86D_001612"
                     /pseudo
     CDS             complement(7245..9941)
                     /locus_tag="ACG86D_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(10369..11442)
                     /locus_tag="ACG86D_001613"
     CDS             complement(10369..11442)
                     /locus_tag="ACG86D_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140754.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11439..12266)
                     /locus_tag="ACG86D_001614"
     CDS             complement(11439..12266)
                     /locus_tag="ACG86D_001614"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140755.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12267..13073)
                     /locus_tag="ACG86D_001615"
     CDS             complement(12267..13073)
                     /locus_tag="ACG86D_001615"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3140756.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13085..14170)
                     /locus_tag="ACG86D_001616"
     CDS             complement(13085..14170)
                     /locus_tag="ACG86D_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140757.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14187..14729)
                     /locus_tag="ACG86D_001617"
     CDS             complement(14187..14729)
                     /locus_tag="ACG86D_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN3140758.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            14987..15976
                     /locus_tag="ACG86D_001618"
     CDS             14987..15976
                     /locus_tag="ACG86D_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN3140759.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15973..16710
                     /locus_tag="ACG86D_001619"
     CDS             15973..16710
                     /locus_tag="ACG86D_001619"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN3140760.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16707..17258
                     /gene="coaC"
                     /locus_tag="ACG86D_001620"
     CDS             16707..17258
                     /gene="coaC"
                     /locus_tag="ACG86D_001620"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN3140761.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17274..17906
                     /locus_tag="ACG86D_001621"
     CDS             17274..17906
                     /locus_tag="ACG86D_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3140762.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            17991..18638
                     /locus_tag="ACG86D_001622"
     CDS             17991..18638
                     /locus_tag="ACG86D_001622"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3140763.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18643..19626
                     /locus_tag="ACG86D_001623"
     CDS             18643..19626
                     /locus_tag="ACG86D_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN3140764.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20029..21543)
                     /locus_tag="ACG86D_001624"
     CDS             complement(20029..21543)
                     /locus_tag="ACG86D_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN3140765.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21681..22487
                     /locus_tag="ACG86D_001625"
     CDS             21681..22487
                     /locus_tag="ACG86D_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140766.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6787 a         4829 c         3901 g         6971 t
ORIGIN      
        1 tcaccaaaaa aattcaaaca ttaagccaag gattatgaag tcaacatttc gaaaaatgaa
       61 catacattgg taagcaacta taactattac cacagacata aagtaacaca atatattact
      121 atttaagtgt acgccaccag tgaatatcct caaggtatac agcgcacaac aaaaagaaaa
      181 aaggaaacgc agaagcattt cctttcagtg atttaatgaa aacaacacga tttgacttag
      241 agccttttta tttgaattca ttttcgcttt atccaacttc atttgatcat ttgattactc
      301 ggttaaatac ttttgacatg agtatcaatg aaacattcat taattatggt tgcttatttc
      361 ttaccgcttt tctggccaat gctaatccgc cgattattcc ggcattatca cctaattcgg
      421 gcgtaataat gtagctatca attggtggta tttcaacata tccatttata agctcaacaa
      481 atttatcctg aacttttttc ttcatgtgtt cttgtttcat tacaccaccg ccaaaaataa
      541 ttctatctgg cgaaaacatc agtgttgtat tgtatgcaca ttgggcaagg taaaaggctt
      601 caatctgcca aaacgaatcg tcagccaata aattctggcc ttttacaccc aaccgttttt
      661 caatcgctgg tcctgctgcc atcccctcta aacaatcttg gtggaaggga cagtttcctg
      721 aaaaattgtc ctttggatgt cgcttgatca acatatgccc catttccgga tgactgaacc
      781 cttccacgaa agtcccattg aacaaggcac cacctccgat acctgttccc acagtgaagt
      841 atgcaacacc tgctaaccct ttgccagaac cagcaacata ttcaccatag caagccgcat
      901 tgacatctgt tgtccattca atcggaatat tgaaatattg ctttagcgtt cctacaaaat
      961 tgaaattttg ccaagcaagt tttggtgttg atgtgatatg accgaaactc gttgatttgc
     1021 attgaatatc aattggacca aagcagccga ccccgatgcc tgccaattgt tccttaaaag
     1081 gtttaaaaaa atcaatgacc tgcttcattg tttcaactgg agttgttgtg ggaaaactga
     1141 ctctttccaa aatcgttaat tcctcagtac cgactgcaca gacaaattta gtccctccag
     1201 cttcaattgc cccatatatc atcataggtt cctcccttcg aatatgtgaa aaacacttca
     1261 ttaacgcggc aagcgttctc gatcagtgaa atgacgttta gtatttcctg aagcttctgt
     1321 tgaataccag taagcgacac tagcaacatc gtcttgccgc tcaaataagc gaacatcatc
     1381 gttgccgatt tgttgtaaag tgaccgataa atcttcggta aaacagatgg ggtcttgaag
     1441 atgccaacga taaagaccat gtcgtggcat tgcgtcgtca ccaaaggcat gaacggcatt
     1501 caattttccc gtttcaaaac gatctcttgt ggcatctcta tttgattggt aaggataccc
     1561 taaaaaggct gttgaatagt ttttcgcatg tggacgtccg aactcatcgt actcatgaaa
     1621 ggcccacgcc ccgccaaaat aatcttctga acctgttgag gttacggtcg gatactcact
     1681 atccccatca atgtagaatt taaactcgcc ttcgccccac caataacgtt ctaggcaggt
     1741 aagcgcaagg taagtaccca cgtaatagcc ttgacccttt aatcgatcaa ttaacttata
     1801 atcctcctgt aatttggtaa tgcgttcccg atgccaattt gcgtgaaaat ataggctatt
     1861 ttcttccaat tcgtccacca aagcgtaatt tatcgtataa aaaaatgagg acatatcctt
     1921 cggatgtaaa ttctcgatcg taattttagc ggatgtttgg aaaggcattt cgaaatagga
     1981 attaaagccc cctgtaggat taaccactat cggcaacgag ttcacctcgc aacgctctcc
     2041 aaacccatta caaaagaaat cccctaaagg actttcaaca gcagggctat ccgacccatc
     2101 ccaataaatt cttaagatta aatcacgtaa aacaaaactg ccaaattctg ttcgatcacg
     2161 aatcgtcatc cacatatgac gaattattcc cgttccagta atatccgcaa tcacagtttc
     2221 ttcaccagaa gtcagcggaa ttgcgccacg acctttgcgg cttggtccta gctcgcttgg
     2281 caacatcgca gctttacctt tctcgcctgt tggattttcg ggagtgattg ctcgactctg
     2341 gattttttta aattgggtaa tattttttag tatactcatc tttttctcct ttgaatgtga
     2401 atcgtttttc ccttttctac gtgaagaatt tttttcgtat cacttatttt caatggcttt
     2461 tctgacaaat ttgaaatact caactcggat tgcgtacatt caatcgaaat gaaggcttga
     2521 ccgaacttca aattcttcca agaaaacgtt aaattatcaa caggatcaat ttgcaatact
     2581 ccttttttga atttcaatcc gaaatattga ctgatcatta ggctgacaaa aagacccgat
     2641 gcccagccgc atttgccgca gccaaagtct cgcacgactt ctccttgttg agcctctaag
     2701 cgatacggcc accaccacca cgaaccgtct aaatctgcca aacaaattag ttcttctaat
     2761 cgggttacga aagcttgagg gttgtgcagc tcgctcatta aaacagtgat aaagccagga
     2821 aaagtggcgc cggattctaa tccccaagtg atgcctttga tttcctcgct ataggttgga
     2881 ttgaaagaac tagcagaaaa ggccatggtg ttctgatata aatgatcttt attatctaaa
     2941 aattgataaa aaggcatcaa cgtagtgtct gattcttctc catcatgcat caataaatta
     3001 atttttccat ccacaatcac atcacttaaa aagattaacc cttgctcaag aatcggtttt
     3061 tgatagtgct tcactgaata ggttgttttc tcctcatcac cgattccctc caaaaattgc
     3121 tcaccaaaag gaccatcgat agtcatttgt ttacaaattg attcctttag tttacaggca
     3181 atgtttttgt atcgcttgct aagactcagt tgttctaggg cttcaaaaat ttctgcaagt
     3241 ccaatacagg ccttccaaag acaaagattg ctgccggtat gatactttcc aagtgcaaag
     3301 gcgtctgata accaaacaga tggaaaaagc attggttccc ctgaccgacg ttgagctaat
     3361 atgtcctcaa taattcttga agcattcagt agaacctgcg gacaccgctc aaagaatttt
     3421 gtatccccag tatgttcaaa ataaagacta gccagtaacg cactggataa cgaattactc
     3481 agagaatgat tgatacctcc tgtaaatttt gaaccaggaa acttaacgcc atattgatca
     3541 aaccatagaa ccgttttttg acataactcc ggttcaaaca tggtgaatgg caatgatgcg
     3601 taatacatat ccttattcca aattcgatta gtagcaggat agctgcccca attgctgccg
     3661 acgacctgtc cctgtcgatt cataccgaaa ctagaaaagc tttgataaag tgcacgacga
     3721 tagagatgga caactctatc gtttggcatt gacagctgcc caaatagctg agcaaaatag
     3781 ttcaatgttt ccttcagcca tttttcgata tcagattcct gaaaagtcat tacttcttca
     3841 aaagcatttg ggtccacaaa agcaatggcg aagcttgcca cttcctgttg tgcgatcgtg
     3901 tccttatttc ctttttgaga aatcaacaca ttttgctgat ccgaatactt ctcttgatac
     3961 gaaggcacat ctttcactga aacttgaagg ctttcgtgtg tttcattcac aatgtaaacc
     4021 tgttgaacaa gcatacttaa acgcttatta gccagtatgg gagcaaagga tactacaatg
     4081 accttaaacg tcgaataatg atgaatatat ctaggcaaac agtcttgaat cacatcgact
     4141 tcaatacgat gatccgattc tgacaaatgg tagacaatcc cgtttacttc aacctttaag
     4201 gataacgaat tggtttgaga gagctgttta ttgacccaaa cacctggttt ttcctcagtt
     4261 agtaaatttt ctcgataaaa ggtcaacgtt tgaatcgtcc cgcaaacatc aaggtttgct
     4321 aatgtctttt gattacttac atcgaaggtc aatcccttta catcaggaat attccgatag
     4381 tagctgtatt cgtttgaagt agtcacattt ccgtcgttta actcagcatt aaaaaatggg
     4441 tgtttgggtg aataaattac ttcattaata gtcatctttt ccctccttgc taaaaaaact
     4501 gcagcctagt agctgcagct tcctactatg cgattccaag taatgacaat aaaattccca
     4561 agacgaccat tccaaaaata attttgatga cgccgacttt tttatctagt aatttgaaac
     4621 agctcaatgt taccaataac ggtacaattc ctttaaaaat actatcgagg atttcttgga
     4681 cattcattag ttcttcgccc ttcattgaaa agctcaaaac ggtattgaat ttgaccatac
     4741 tgatcgtcat tgcacctacc atgatcagac ccaagatact agcagctttt gtcagaattt
     4801 caattaaccc gttcgcatac agcttttcaa tatatttaga gcctaatgaa tagcctaaaa
     4861 cgccgccata gtatcgcatc aagtaattag gtatattgaa cataactaaa aagataattg
     4921 gtgccaaaat atttccggca ttggctaagt tgatcgctag tcccgcggta acgacacgcc
     4981 aaactcccca gaacaatgaa tcgccaattc cagccaatgg tcccatcaaa ctcgttttga
     5041 tcgcgttgat actttccacg tcaaaaccag ctttttcaga attttccttc tccatcgatg
     5101 ccgcaatccc catgacaaag gacgcgcaag gaatggtcgt cgaaaaatac tgtgtatgac
     5161 gttccagcgc cgcagctcga tcttcttcac ttttgtagaa atgattaatg aagggcatca
     5221 tagaataggc gaaaccaccg ccgcccatcg tcaccggatt caccgaagca tacatagtca
     5281 tcgagcgcca aaacatcttc cgaaccaatt ttttctcatc tttagaaatt ccatcagcta
     5341 atttcattga aagaaatcct cctcttcttg gtcaagcgtt gcatttacag cagcatcgct
     5401 ttgaactgca tgaacagata tattttttag ttgtagatct ctttgggcta taacaattgc
     5461 aataatcgtt ccaactgcgg caattgcaat caatggtaac tcgagatacg ccataacaac
     5521 ggccccaaag aaaaaccaca cagcaatatt tttactccaa agcatccgta acagcatcgc
     5581 catcccaact gccggcagca gatttccagc gactgttaat ccgttcatga tgacctctgg
     5641 aatattatcc aagacgcttt tgacaacatc tgctccaaat aaaacggcaa agaatggaat
     5701 aagagcataa agcgcatagt ggacaaacca cataaagaag tgcagattga tgattccttt
     5761 ttgatcacca gattttgcaa atttgaaaaa tttagcagcc atcggtccac atacaaaaga
     5821 aaaaatcata ttcattaact gtaatcctaa tactgctagt ggaaagacaa tgggaataat
     5881 cgcatccacg tttcctccca ttttgattgc gaacgctgta gccagcccag cagccaaaga
     5941 aggctccgcc gtactagaga ttccgatatt gacgacaccc ataaagatgg cttctagtga
     6001 cgccccgaca atcaaccccg tctgcaaatc ccccatcaac gctcctgcta acggagcaac
     6061 aacaatcgga cgactcagca tggacatccc gaacatttca tgtccagcaa ctccaataaa
     6121 aacggccaaa gcgactacaa atgcatcata cataattttt ctccttcctt tttctaaaaa
     6181 tcactcaaaa tagagatagc tgaacgtttt tccatcgccg gtgtaggctg aaaaacggta
     6241 tctggataaa gttcaactaa ttcttttaag tcttgaattt ctgacgtcgt taacatcact
     6301 tcctttgata aggttttctt atcttccgat ggcggtgtca ttttcccagc attccccacg
     6361 ttcattaatt cgatttccgt tgggaaagct ttcgcaactt ttaccgcatc tttgacatta
     6421 gggactaaaa ccatcaagcg catgtccttc gcccgcggat cttttaacaa atgaatcgcg
     6481 tcatcaactg ttttaataat gactttgaca ttcgatggtg cagccatctt caatgtcatc
     6541 ttttgaatat cgttggtagc cgcctcatcg cttgctacta ctaacccggt taaatttaga
     6601 ctctttgtcc aagtcatcgc tacttgtcca tgaattaatc gttcatcaat tcgcactgct
     6661 ttaatcattt ttcttcctcc taaaaaaaat cttcctcttc atttactaca tgtacctcat
     6721 caagaatggt catctttaat tcctgttggc atttatttac cagttcctga atatcctcct
     6781 ttgttaattc cttggttgcc aatactattt cgagcagaat tggcagatta aagccagaca
     6841 aaatagttac agcaatctct ttctccaaaa aggttttggt aatcttctga ttgacacttc
     6901 cgccaaataa atcggtaaaa acaaaaatct gatcctgctc gttcacctgg tttacaaata
     6961 cattcaactc ctgttcaaaa tcacactcat ccacataagc attaatcacc gtaatctctt
     7021 gaaccttccc cgtcagtaat tgaatactgc taagaaagcc tgaagctaag tgaccgtgag
     7081 tcgctattag aattttcttc atatggttaa ttcctttctt tctgttcact tttatataag
     7141 caattcctgt gccaactttt aaaaacattg gtttaatcac gttaattcac attaaaaaaa
     7201 gtgtctaacc taaaaaaaac agattagaca cttttttaga cactttattt ttgaatatac
     7261 ggtcctaata attcatagag cagcgaaagc tcataagcat tgactttaaa attatatttc
     7321 atctccattg attgaaaaat acttctagaa acataataaa attcatcttc atctggtttc
     7381 aaatcataat tcggttcttc agatttttct gtccgagcca agaataaacg ttccatcatc
     7441 aatgccgtat gcatatagag atttagtttt acacggccat ttaaagtgaa atgatagtaa
     7501 ttttcatatt tagaaatgac tgtttccact tcactcatca cgatcgttgg gttcagaaag
     7561 ttcaaacggt ccgcaattcc ttccaacgaa aagaatttta ctagctcctg aatcaagcgg
     7621 ttaaattctg ccggcttcat ttcaggactc aaaatatgct ccaaattggc tgctccatca
     7681 ccgtctaaga tgtcataaac attaatatta ggaattgaaa aggaacgtgg aagatccgaa
     7741 gtcgtgatga ccaattttgt cttgtcaaaa taagtttcat cattttggtc gattaattca
     7801 cgtagttctc tgtagtcctt cgtgaaaact tcgatggatt catccgataa atgatgacga
     7861 aaaacatctt tcaatttgtc cgaaatcccc aaacccgaca tacaggaaat gataatgttg
     7921 ctactttgag aaaagccttc aaagtattga acattgattg aatacttgtc tgctgcatcc
     7981 tccgcaatct atttaaatgg cgcattcatc gtcatcttca agccaatatc aagtgcaatg
     8041 gaagtcgtca aattattcag aagaagtaac tcccctttta aatcgttctt gatctgtgtg
     8101 taaatttgat ttaaagagcc catatctacg atcagcacta gactatctgt attcacttgc
     8161 cgtttcacga aattccttgt cgattcgaca atctctgaca cattactctc tactggcata
     8221 tccaacgctt caaaaacata attgccgcat aattgattca ctactgcttg aatgctgcta
     8281 gccgtactat ctccgtgagc aagcaacagg cccttcagct cgatggattc atctacataa
     8341 tcagataaag tcacgacaat aagcagtctt agaaagttct cgtccttttc tgagagcggt
     8401 ggtaaatgct ccaagaactg attacataag taagtcgtcc gaggtagatt tgcagctaat
     8461 gtacgattta tcccggttaa gtctgtctta tagcttgggg aaatgacata ggatctgcta
     8521 ataatttctg caacaggtcc actcgtcgtc aatccgtacg tttttcctaa aacggatacc
     8581 cagacctttc taaacatctg aatcatcaat tttctactca ttgataaaaa agaatcctta
     8641 tcaatgtgat gcaccaaatg ctgtacttca ttctttatcg aagaaaaatt ttcattgaaa
     8701 ttaccttcat ttatcagctg agtaattttt tccaaaatcg aatcataatc aaacggtttg
     8761 attcgatccg tttttatctt ttgatttttc tttatcaata aactagcaat cggcaagctt
     8821 tctacttcgg attccagcat atttaactga atcaagatct catttttccc aatcgttaat
     8881 ggttcttctt gtgttcgact atacacatcc gcacaactga ttttaatgat attcttcagc
     8941 ttaccaatat tcccactaaa gggatgagat aacattaaag acaaggcttc tcttgaaaca
     9001 ataatatctt tttgtaaaac atccgcttca ttttgaaaaa ataattgaat cagctctaat
     9061 cgttctcgaa tggggcgatc ttcaaaagtt ggcaacataa cactaatttg aatacgccga
     9121 tcaaaggtgt ctaataaata attttcacct ttttccgtcg tggcgaaaat aaaacgaacc
     9181 ttagcagaat gccaatttac attttcaccg actggacgat agtttccagt atcaataaat
     9241 agaaagagct tttcttgatt ttctttcgat aggcgatgga cctcatcgag aaaaagatag
     9301 ccgttgttgg ccgttgccaa taaaccatcg gtatcctttt cagcccccgt aaaggccccc
     9361 ttcttgtatc caaacagtgt tgccgaaagc agttcgggat tgtccgcata atcggcacag
     9421 tttaagatag caaatggtgc atctttttca ataatttgct catggatagc gtattcaaaa
     9481 atttttgtgg ctaaaaaact ttttccaaca cccgtggctc catttattaa tacactgagt
     9541 ccgttaggcg gatatttgac agcggctata catttttgaa tgacctctct cagacttccg
     9601 tcatgaccaa ctacagaatg aaaggcttcg tttatttgct ccgtattatc tacaaccgca
     9661 tattcatcta aaggcttcca caaaacaggc tttcccggta atttctcgac ctttttatct
     9721 tccactaatc tcgtcaaata atggctggca ttctgacgag agatacctag attcttcgaa
     9781 attttctcgg cagtgacaga agacatattc cctgttagtc gtatcagctc atcaaaaact
     9841 cgatctttag cgctcttttt cattttttcc tccagtttaa tgtgtctaaa agcataataa
     9901 actatcggct gcaatatgtc tattctatat ttgtattcca atttttactt tgaaacacaa
     9961 atagtcgaat atttgcctca gatatctgac aaaataattt atctaccaat ttagttttta
    10021 tctaatatga tttttatgtt ttttcatagc tacaaatatt gaactggatt catatattag
    10081 ttttattaat agattacaga agggttaagt atatttgcat agtttccaat tactttatcc
    10141 ttctgtaact ttttcaatct tagatttact agcccgtctc gggccctatt ttacaatggt
    10201 tttgtccact tttctctggt agaaaaacgt ggtagaaaaa ataaaataac acaaaaaaag
    10261 cggcgtttca tccgcttttg gtagaagatt ggaaaactca gatagaaaaa ggacgatttt
    10321 tagctcttta gaacgttgat aagtccccat tcttactgag ttttgccatt atctcctata
    10381 catcttgaat tctaagaata ggtcattata gatgcctagg tatttcgatc ctagatcttt
    10441 gtagacttca agatgggaga tcgtgtcatc agatggatag aattgttcgt ccgacgaaat
    10501 gtcttttggc aataacgctt ttgcttcttc atttggtgta gaataaccaa tatactctgc
    10561 attttgtgcc gcattttccg gttctagcat aaagttgatg aaatcatagg caccttcttt
    10621 atttttcacg gtttttggca taacgatatt atcaaaccaa agattggatc cttctggtgg
    10681 gatcacataa tgtaaatgtt cattttccca catcatatcg gcagcttcgc cagaaaatgt
    10741 cactgccacg gaggcttctt cattgatcat atacattttg atctcatctg ccacgattgc
    10801 tttgacattt ggtgttaaat gattcaattt atttgctgct tctgtcagct gtgccatatt
    10861 cttactattc aaagaatagc ctaaactatt caaagaaagc cccataactt ctcgtgcacc
    10921 gtcaatcagc atcaaactgt cttttaattc cggcttccac aaatcatccc aatgcttaat
    10981 ttgatttgca tcaaagaatt tatcgttata tacgatccct aaagttcccc aaaaatatgg
    11041 aatcgaatac tgattatctg gatcaaaatc caagtctaaa aatcgtggat caatattatt
    11101 taatcctttg atcttcgaat gatctaatgg aagtactagt ttttctttga tcattttttg
    11161 gatcatatat tcacttggta tagcgatgtc gtagtttgtc ccaccttgct ggatttttgt
    11221 gaacatcgct tcattagaat caaatgtttc ataattcact ttgtatccat attctttttc
    11281 aaatttcgtg atcaaactag gatcgatata atctccccag ttatagatgg taagtacctt
    11341 tgctccactc atgccacttg ttttttcaag ttgacgaaca ccaagcagca gcaccgcaat
    11401 aatgatgaga atacctaaaa acagagattg taattttttc atcgcagccg tgccaccccc
    11461 tcgcgtactc ttctgttttt cttcagccgt tttggacggt tttctttact aataaagtag
    11521 tatccaatga ccaatatcat ggagaacaag aaaactaacg cacttagcgc attgatttct
    11581 aaactgattc cttgtcttgc acgagaatag atctctactg agagcgtcgt aaagccattt
    11641 cctgtgacga aaaatgtaac cgcaaaatca tcaagagaat aggtaaatgc cataaaatat
    11701 ccagctatga ttcctggaga aagataaggc aaaatgatat ttttgactac ttggaaatta
    11761 ttagcaccaa ggtctctagc cgcatcgacc attgaatcgt tcatttcctg caattttggc
    11821 aagaccatca agacgacgat tggaatgctg aaagcaatat gggaaagcaa tacactctga
    11881 aaacctaatc cgaatcctag tagtgtaaag aaaatcaaga aactagcacc gataataaca
    11941 tcaggagaga ctaataagat gttattcatg ctcaacaacg ctgtccgtgc acgtcgtttt
    12001 ttcgtgtaat agattcccat agccccaaat gtcccgatga tcgtggcgat caatgcagat
    12061 aagaaagcca gcatgaacgt attcaatacg ataatgatca aacgtgtatc ttcaaaaaca
    12121 gcttcataat tttcccaagt aaatcctgta aagctgttca tcgttcctcc ttcattgaag
    12181 gaataaaaaa tcagatagaa gatcggtgca tataggaata aaaagacgat ccctaaataa
    12241 agatacgacc atttaaattt tttcatttat gagcgacctc cttttttctt ctcgcctgtc
    12301 aggagcatca cgatgaacat agcaatgatt aaaatcacac cgattgtaga acccatcccc
    12361 cagttttgtg taaccaagaa atgctgttcg atagccgttc ctaaagtgat cacccgattt
    12421 ccgccaatca gacgagtcag catgaataaa gaaagagaag gaataaatac tgcttggaca
    12481 ccacttttca caccatttaa tgataacgga aaaatcactc gtcggaaggt ctcaaagcta
    12541 ttcgcgccta aatctctact agcactgatc aaagaaggat tcatctcttc caatgcattg
    12601 aaaattggca taatcataaa tggaatctcg atatacgttg ccacgaacag aaaactgaag
    12661 tctgtgaata atatctgttt cggcccgatc ccgataaaat caaagaaatg attcacgctg
    12721 ccgtgcacac tgaaaatccc aataaatgcg taagctttaa gcagcagatt cacccatgta
    12781 ggcaaaataa tcagcatcag ccataattgc ttgtgcttca gttttgttaa aaaataagca
    12841 gtcgggtaac tgatgatcag cgtgaaaaac gtgatcaaaa atgcatacca tacagaattg
    12901 atcgtcatag aaagcgatgt ccccgacgta aaataagata gataattatt cagcgtgaat
    12961 tgcccattca tatcaaaaaa agattgataa atgatcatta atacaggcgc gatcacgaat
    13021 aaaccgagcc acaaaatata agggacggaa tagatcctgc gcattgtttt cattcttgtc
    13081 ccccctattc ttcgtagctg tcaagacgtg catcgaattc ttcttcagac tcgttgaaac
    13141 gcatgacatg gatatcttct ggctcaaagg ccaatcctac tttgcttcct tctttagctt
    13201 ttttcgttga atggaccatc cattcatttt gctgttcatc ataacagatg atttcataat
    13261 ggacaccacg aaacagctga gtatcgaccg ttacgactag ttttccttta tctggtgtcg
    13321 tgatcgtcaa atcctctggg cgtaaaacaa cttcaactgg ttcatttaga cgcatcccgc
    13381 catcgacaca ttcaaattgc ttaccaacaa actcaactag attatcttcg atcattacac
    13441 cgtccacgat attgctctcc ccaacaaaat cagcaacgaa atgattgatt ggctcatcat
    13501 aaatatctac gggtgtacca ctttggacga ttttcccttt attcatgaca aaaatctcat
    13561 cactcattgc caacgcttct tcttggtcat gagtaacaaa aataaacgtg attcctaatc
    13621 gttgttgcag atcccgcagt tcatactgca tatctgtacg tagtttcaaa tcaagcgccg
    13681 ataacggttc atctaatagc aatactttcg gttcatttac aattgctcgt gcaatcgcta
    13741 cacgctgtct ttgaccacca gacatttcac taatctcccg tttttcgtat ccaggaagct
    13801 ggaccatacg caaagcatct tttaccttct tttcgatatc tgatttggac attttcttga
    13861 tcttcaatcc aaaagcaacg ttatcaaaaa cattcatatg aggaaaaagt gcataatctt
    13921 ggaagactgt atttacttgt cgcttattag ctggcaaatc attcaaccgt ttcccttcaa
    13981 aatagatgtc cccttctgta gcttcggtga atccagctat aatacgtagg atcgttgttt
    14041 taccacagcc ggaaggacca agcagtgtat aaaattttcc ttgttcgatc tcaaaactta
    14101 ctttttttaa tactgtttca tcatcatagc gtttcactac ttgatcaaat gaaatgattg
    14161 gtttccccac gtttttaccc cctatattac aaatatgatt gcgttgcaac aatcaatact
    14221 tttgttcgtt ttttgagatg attccttaat tgatgttgtt ttgtcgcttg ataatagatc
    14281 gactgtcctg cttttgccac atagaccgtc tctcctaagc ttaactctaa ttccccttct
    14341 aatacaaaaa taaacgtttc agataatgaa ggttcaaacg tcttatattc tcctgcacct
    14401 tcaaaagtca gcaagacagg ctccatttct ttttcattcg aatcagccac gagccattcc
    14461 aattcatacc cgttttcttc atcgaaataa accgtatgat cttcttttga ataaaccact
    14521 tgcagattcg ctgcttcttg atgaaaaaat tcttctggag tcacacctaa gacttcaaga
    14581 atagaaaaga atgtttccat tgatggtgaa cttaaatctc gttccaactg ggaaatatac
    14641 ccttttgaca aatctgtgcg ttcgcctagt tcttcttgtg tcaaattctt ttggatgcgc
    14701 aaatttctta acttctcacc aatttccata atttcctccg aatcgaagtt ttctaatagt
    14761 aaacttcctg tttactacca ctcgtattag tatacgaaat ttattgaaaa aggcaaccct
    14821 ttgtaaaatt ttttttgctg aatatttctt tatcttttgg aataaattct agttgtttga
    14881 ttctttattt ttcacctaat ttttgtctaa atttacggaa gaacaagaat aaaagagtag
    14941 gctttatggt atgataaaag aaaattttaa aaggagtgtc tcacgaatga acgcttttcg
    15001 atcttttcag gtaataaagg aacctgtttt ccatacagca atcagtgatc tgccatttac
    15061 tgagttggct gaaaatgaag tcttggtaaa aattgcctat tctgatgtca attataaaga
    15121 tgcattggca atgagcgagt ctggtcaggt gatacgaaca tatccgatga ctccagggat
    15181 tgatttatcc ggaactgtcg tccaaagtaa caatccacgt ttttcaaaag aagatcttgt
    15241 acttgctact ggttttgggc ttggcgtcac ccatccaggc ggttatagcc aatatcaaaa
    15301 agtaccagga gactggttag ttcctctacc aaaaaatatg acgctgcgtc aagcgatgat
    15361 ccttggtaca gcagggttta ctgctatgtt gtgtgtgaac gctttaatcc gtcaaggtat
    15421 gacctccgat aaaaaagttg tcgttactgg tgcttctggc ggtgtaggca gtacagcgat
    15481 agccctgtta cataaactag gattccagtc aatcattgcc ttttccagaa aagaagagtc
    15541 tgtcacatgg ctaaaaagtc taggtgcttt gcaagtcgta cgacctgatg aatttctgcc
    15601 agagacaaca aaagctttag gaaaacagca aatcgactat gtcatcgaca cagtaggtgg
    15661 cgagcagcta agcagtcttc ttccattgat ctcttataac ggtgcagtag ctttatgtgg
    15721 caatgctggg ggaatcaaac taaacgcgac tgtcttgcct tttattctgc gaaacatcca
    15781 gttgattgga attgattctg tcaatgtacc aatagatcaa cgtctttctt tatggcaaca
    15841 gatggcggat ttgcagattg cagatgaact ggtcgtccaa gagattactc tggatcaatt
    15901 gcctgaaaca gcatctaaac tacttgctgg aacacaccaa ggaagaacac tagtaaatgt
    15961 aggtgaccat aaatgaagat tctaatcaca gctggaggaa cgactgaaaa aatcgatcaa
    16021 gtacgtgcta ttacaaatca ttcgacagga cgattagggc aagcattagc tgattattta
    16081 gctgccaatc cggataccac aatcgattac gtgactacga aacaagcact gaaaccgaaa
    16141 agacactcca atataacgat ttatacgatc gaatctgcac tggacttatt tttacaatta
    16201 gaagaactaa cgaaaaaaga acactacgat gcgattatcc acagtatggc agtcagtgat
    16261 tttactcctg cttttagttt ttcagaagaa cagctggcaa aaaacctacc cacttcttca
    16321 acgcaagaag aactggacaa ttggtttgcg gaaaatgaac agacagatac cactgtttca
    16381 aagatctctt ctaacaccga gcatcttgtg ctcgttttga agaaaacacc aaaaatcatt
    16441 tcttatctac gtgaatggca gccaaaagca aaaatcattg gtttcaaact attagtagat
    16501 gtaccaaagg aatctcttct ggcagtcgca aaaaatagtc tgataaataa caaaactgat
    16561 tttattttcg caaatgattt gacggaaatc catggcgaga cacaccacgg ctatctctta
    16621 tctaaagatg gaacagtgga agaagctcaa agtaaatctg agattgctgc attgattact
    16681 gagaaaatta gactggagga atcaaaatga aaaagatctt actaggcgtt actggaagta
    16741 tttctgctta caaaagcgct gatcttacaa gtcaattcgt caaattaggt gcccaggtag
    16801 atgtgattat gtctaaaagc agcacacatt ttatcacgcc attaaccttg caatctctgt
    16861 cgaagcgcgc agtccataca gacgtcatgc aagaatcaaa tccgcaggta atcaatcata
    16921 tcgaactagc aaaacaagct gacttgtttc tcatcgcgcc tgctacagca aacattatcg
    16981 gcaagctagc aaacggcatt gcagacgatc ttctttcaac agtcgcaatg gcgcttccag
    17041 tgggaacacc aaaattgatt gcccctgcca tgaacacgaa tatgtatcaa aacccgatca
    17101 ctcagcgtaa tctggaaaca ttgagatctg tcggctatca ggaaattgaa ccaagagaat
    17161 ctcttttagc ttgtggagat tttggaaaag gtgccttagc tgatattcag acgatcatcg
    17221 aacaggtcat gctgactctt aatgaaagaa atgcttagaa aaaaggagta cttatgaaaa
    17281 atacaaaaaa ctttacatta actgcgatgt ttttagctat tttgatttta ttagctgtta
    17341 cgcctttagg ttttatccct atcggtccaa tcaatgcaac aaccatgcat attcctgtaa
    17401 tcattgcttc gatcgtttta ggaccaagac taggtgcttt tcttggcggt acatttggac
    17461 tgatcagcat gatccgtagc acagttatcc aaacaccttt gtcttttgtc ttttctccgt
    17521 ttatcccagt catcggaaca gaacatggaa gctggaaagc cttgctgatc gcgtttattc
    17581 ctcgcatctt gattggggtg gttccttatt tcgtttataa aggtctgcgc aaactaatga
    17641 aacaaaaagc agattccgtt tctttattct tagctggact ttccggttca ttgaccaata
    17701 cgattttagt gatgaatatg atctactttt tattccagca agattatgcc aaagtcatcg
    17761 gaacaaattt aaatgctgtt tattcagcta ttctagcggt tatttttaca agtggtatac
    17821 ctgaaggaat cgtcgcaggt cttgcgactg cagcagtatg caatgtcttg ttaagatact
    17881 tcaagcaccc ttctcttact aactgatagt catcgtagcc gatctctgat ttttttctct
    17941 tttggtgtta ctattgaatg gaaagatttg acgtcgaaag gatgatccaa atgaaaattt
    18001 ttgttgtagg tgcaaacgga caaattggcc gacacctgat aaaagattta gctccaagtt
    18061 cccatgaaat ttttgcaggg gtgagagatg ttgcttccca aacattagta aaaaaagaga
    18121 atgtttcgta tgtctctttt gatttaactt ggtccgtgga aaaaatgacc gaagcattca
    18181 aaggcatcga tgtattgatt tttgctgccg gctctcaagg aaagaatctt ctgcaagtag
    18241 acttggatgg cgcaatcaaa acagtgatcg cagctgaaaa tgcgcatgtc tctcgctatc
    18301 tgatggtcag tgctgtttat gcagatgaac cagcaaaatg gcctgagtcc atgacagatt
    18361 attatattac taaacattat gcagatgaat ggctgaaacg aactaactta gattttgtca
    18421 ttcttcaacc agtcacattg actaacgatg aagaagttac ttccattcaa ttaactaagc
    18481 ctaatgagaa ggcatcaaaa acgatcactc gctctacagt tgctgctgta cttgccgctc
    18541 tagtggaaga aacggatatt tctcgtacga ctctagtact ttctgaaggt agcaaagagt
    18601 tgaatacagc attccaagaa tgggcaaagg aggaatagat ccatgcgtgc catttcaatc
    18661 aagcatcctg gttctgtaga tgtattagaa caaattgaag tgcctgttcc taaaccaaaa
    18721 caaggcgaat tactgatcaa agtccataca gcagctgtca atcgaacaga tattatgaaa
    18781 agagaatcag tctctttgaa acctccctac cctattttgg gagtcgaagt agctggagag
    18841 gtagttgaga ataaaagtga tatctccact tttactcctg ggacaaaagt atacggatta
    18901 gtcaatctag gtggttatgc cgaatatgct gtgatgcctg ccgatcgtgc aatccttctc
    18961 cctgatacgc tagactatgt ttctgctgct ggaatctcag aagttttttt gactgcttat
    19021 caaacacttt actggcttgg aaaattacaa gaaggcgaaa ctgtcttgat ccatgcagga
    19081 gcaagtggtg tgggaacagc agcgatccaa ttagctagac agctttctca cgcgaaaatc
    19141 atcgttactg caggatcaga agaaaaactc gccttttgta aagaacttgg tgcagatgaa
    19201 ttgatcaact ataaaaaaca agactttcaa gaagaggtac gaaaaatcac tgaaggtcgg
    19261 ggagtcgatg ttattttgga tttcatcggc gcttcttact gggaaaagaa tttagcttct
    19321 atagccgttg atggacgttg ggtcttgatt ggtatgctag gtgggaccat cgtgcctgag
    19381 atagaccttg gtgtattgat tgggaaacgt gtccaactgc ttggtacatt attgacacct
    19441 agaagtgatc agtataaagc agagctcgca caaactttta tgcaaaaagc tgggcctttt
    19501 ctagaaaatg gcaagattcg gccaatcatt gaccgtacat tccaattcag cgcagtcaaa
    19561 caagcgcatg aatatatgga agcaaacaaa aacatcggaa aaatcatttt gaagataact
    19621 gaataaaatc atgctgatca aaaaagaggc tgggacagaa gcgtttaact ccaagaaata
    19681 agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcagctta tttccgaagg
    19741 aatttcttct gcttccgccg tttatacgtg tttagagcgt gagacaaaag tgttctttac
    19801 ttttgtccca cgctcttttt ccgaataaac ggtgttcaaa agcttgcatc tgcggtaata
    19861 agtccaacca agttaaaaac ataagaagct gtttttactt ggctgttctt attacttgat
    19921 gcagggcagc tttttcacaa cctcttcact aaacagtgtt caatcagctg accctcggta
    19981 ttagctcaaa acgctgattt cacaacctct ttttatttac gttctatttc attcattttc
    20041 cgaagcaaat cgagctgctt gtatgcccgc ttgtcgccca aaaataatga tctccgcaac
    20101 tgaatttcca ccgatccggt tgtttccatg taagccacct gtgacttcac cagcagcata
    20161 taacccccta attggctggt tttctttatc taatacttca gtgttcgtat tgattttcac
    20221 accgcccatc gtgtaatgga ttcctggtgc gattttgatc gcataataat tcggagacga
    20281 taagtcatgt tccatggctg tggcacggtt gaattgagca tcttttttct ccgcaacatc
    20341 cgtattccat ccatcaactg tttttgctag tgtttctttt ggtaaatcga ttttttctgc
    20401 taattcttcc attgtctttc cagttgtgac atatcctttt tggtcataaa attcaatagc
    20461 tgtggcacga tcacgcacgc cttggtcaaa aatcaaataa gctgattttt caggcaatgc
    20521 tgtaattgct gcagatactt tatcccttgt atccatttca ttgacaaatc ttttcccctc
    20581 ttgatcgacc aagattgctc cttctccgcg aacaacctca ccaatcaaga ccccctcatc
    20641 ctgctgcaca gttggatgaa tttgtatttt atccatatca acggtttgtc cacctaattt
    20701 ttcaatcatg gtgataccat ctccagttga tccttcttga tttgtcgtaa tataatcagc
    20761 taaatcggga cgatattttt caataaattc tttgctggca ccaaaaccgc ctgtggtaac
    20821 tacgattgct tttccggtga cctcttttgg ttcttctcct tgaacatgta cagtcacttc
    20881 attgaccgtt tccccatttt tcttaatgtc tgtcacatct gcgttgacaa aaatagggat
    20941 ttcccgctca tggacgttat tcaataaccc ctccactaag tatccaccaa tagccgagcc
    21001 atcagacgga cgatgtgtcc tcttttcact catccctcca gtgatcgtta agttgtccag
    21061 tttgataccg ttttgatcta gccaatcgac cgcatcagca gagtgatcga caaagtatcg
    21121 aagcatttct ttatcattcg ttcctccacc accttttagc gtttcttcat agaaggcatc
    21181 attcgagtct ttgattcctt cttctttttg aaattttgtt tctgaagcat tcataccgct
    21241 tgatgattta gatgtgtttc ctccggcaac aggcatcttt tccaaaatta ctggattcaa
    21301 cccagcatct tttgcttcga tagctgctgc catccctgca ccaccagctc caataatcac
    21361 gacatcataa gattctttca tttcttttgg atctgtatat acttgttcag aagcgcctga
    21421 tgatacttcg gcagattcct tcgtactaga acttgtctgc gctgtatttt tctgatctgc
    21481 gccgcatgcg ctcatagcaa gaagtgcaac ggctgttaat aaaccagcaa ctaatttttt
    21541 catttgtatc ccccttttct ccttatacta tattaagtga tttttttcac aatatcaact
    21601 ctgtttttct ttaagtcata tagctttggt aaacacacgc ttcttagtat aattaaggat
    21661 atacatgaag gagttatttt atgaatcatt tgaaaacctt ttttacgaac agtacaaaaa
    21721 ctgcaggtag aatcacaaac ggaatctata ttttattacg tatcatcgtc gttattatgc
    21781 tagtggatca ggtctggaac caacactggg gaaatacaat gcttttatta ctgacattaa
    21841 ttttatttga aatcccttat tttctggaaa agatattaaa gatacagatt ccaaatatgc
    21901 tagaaatcat tttgttatct tttattttta gttcaacagt tttaggagag ttaagtgatt
    21961 tttatggcta tttttcaatg tgggatactt ttctacatgc attcagcgga tttttagctg
    22021 ggggtgtcgg cttttccctt gtttatttgc tcaataaaaa tacaaaaacc atgaacttga
    22081 ctcctttatt actagcagtt gtttccttct gtttttcgat gacagttggt gttatgtggg
    22141 agtttgtgga attttcagca gataaattac tgcaattcga tatgcaaaaa gatacgatca
    22201 tccagcaaat caatagtgtg gagttaagta aagacggaaa cactgtctat catgttccag
    22261 atatcactcg gacaatcatt gaaagtcaaa atgcagatgg taaaacagat aaaacaatca
    22321 tccaaaatgg ttacttagac gtgggaatca tggatactat gaaggattta tttgttaatt
    22381 ttattggagc agttgttttc agtgtatttg gttacctcta tagtcgaaac gattaccaaa
    22441 aatttacgtt catcaagaat tttattccta aaaaaatcaa acgataaa
//
LOCUS       DAZAJZ010000045        22298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000045 DAZAJZ010000000
VERSION     DAZAJZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.045"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..655)
                     /locus_tag="ACG86D_001626"
     CDS             complement(137..655)
                     /locus_tag="ACG86D_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN3140767.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(657..1274)
                     /locus_tag="ACG86D_001627"
     CDS             complement(657..1274)
                     /locus_tag="ACG86D_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN3140768.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1271..2125)
                     /locus_tag="ACG86D_001628"
     CDS             complement(1271..2125)
                     /locus_tag="ACG86D_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140769.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2122..3033)
                     /locus_tag="ACG86D_001629"
     CDS             complement(2122..3033)
                     /locus_tag="ACG86D_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140770.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3074..4408)
                     /locus_tag="ACG86D_001630"
     CDS             complement(3074..4408)
                     /locus_tag="ACG86D_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140771.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4616..5446
                     /locus_tag="ACG86D_001631"
     CDS             4616..5446
                     /locus_tag="ACG86D_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN3140772.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5690..5939)
                     /locus_tag="ACG86D_001632"
                     /pseudo
     CDS             complement(5690..5939)
                     /locus_tag="ACG86D_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6130..6324)
                     /locus_tag="ACG86D_001633"
     CDS             complement(6130..6324)
                     /locus_tag="ACG86D_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140773.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6829..7014)
                     /locus_tag="ACG86D_001634"
     CDS             complement(6829..7014)
                     /locus_tag="ACG86D_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140774.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7864..8205)
                     /locus_tag="ACG86D_001635"
     CDS             complement(7864..8205)
                     /locus_tag="ACG86D_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3140775.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8205..8435)
                     /locus_tag="ACG86D_001636"
     CDS             complement(8205..8435)
                     /locus_tag="ACG86D_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3140776.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8722..9060
                     /locus_tag="ACG86D_001637"
     CDS             8722..9060
                     /locus_tag="ACG86D_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140777.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9265..9827)
                     /locus_tag="ACG86D_001638"
                     /pseudo
     CDS             complement(9265..9827)
                     /locus_tag="ACG86D_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10559..11140)
                     /locus_tag="ACG86D_001639"
     CDS             complement(10559..11140)
                     /locus_tag="ACG86D_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3140778.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11323..11949)
                     /locus_tag="ACG86D_001640"
     CDS             complement(11323..11949)
                     /locus_tag="ACG86D_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN3140779.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11971..13302)
                     /locus_tag="ACG86D_001641"
     CDS             complement(11971..13302)
                     /locus_tag="ACG86D_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN3140780.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13457..13858)
                     /locus_tag="ACG86D_001642"
     CDS             complement(13457..13858)
                     /locus_tag="ACG86D_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN3140781.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13944..14150)
                     /locus_tag="ACG86D_001643"
     CDS             complement(13944..14150)
                     /locus_tag="ACG86D_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140782.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14245..15747)
                     /locus_tag="ACG86D_001644"
     CDS             complement(14245..15747)
                     /locus_tag="ACG86D_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN3140783.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15765..16220)
                     /gene="argR"
                     /locus_tag="ACG86D_001645"
     CDS             complement(15765..16220)
                     /gene="argR"
                     /locus_tag="ACG86D_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3140784.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16318..16935)
                     /locus_tag="ACG86D_001646"
     CDS             complement(16318..16935)
                     /locus_tag="ACG86D_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3140785.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17156..18529
                     /locus_tag="ACG86D_001647"
     CDS             17156..18529
                     /locus_tag="ACG86D_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3140786.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18604..19716
                     /locus_tag="ACG86D_001648"
     CDS             18604..19716
                     /locus_tag="ACG86D_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3140787.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19788..21425)
                     /locus_tag="ACG86D_001649"
     CDS             complement(19788..21425)
                     /locus_tag="ACG86D_001649"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN3140788.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21593..22201)
                     /locus_tag="ACG86D_001650"
     CDS             complement(21593..22201)
                     /locus_tag="ACG86D_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3140789.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6511 a         4539 c         3880 g         7368 t
ORIGIN      
        1 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
       61 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
      121 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
      181 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
      241 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
      301 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
      361 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
      421 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
      481 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
      541 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
      601 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
      661 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
      721 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
      781 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
      841 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
      901 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
      961 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
     1021 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
     1081 gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga
     1141 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
     1201 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
     1261 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
     1321 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
     1381 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
     1441 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
     1501 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
     1561 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
     1621 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
     1681 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
     1741 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
     1801 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
     1861 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
     1921 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
     1981 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
     2041 tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac
     2101 tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg
     2161 tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat
     2221 agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat gcagattata
     2281 aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg
     2341 aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat
     2401 taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga
     2461 ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc
     2521 agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca
     2581 agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga
     2641 aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa
     2701 agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac
     2761 actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga
     2821 tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt
     2881 catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc
     2941 tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc
     3001 ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac
     3061 agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg
     3121 ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta
     3181 atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt
     3241 cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat
     3301 tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta
     3361 cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt
     3421 atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt
     3481 ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt
     3541 aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac
     3601 cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt
     3661 cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt
     3721 tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc
     3781 ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg
     3841 ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt
     3901 taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag
     3961 gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt
     4021 tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat
     4081 ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag
     4141 tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat
     4201 tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc
     4261 agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac
     4321 tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca
     4381 gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc
     4441 cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct
     4501 aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt
     4561 ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt
     4621 tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc
     4681 gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta
     4741 cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat
     4801 tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga
     4861 gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat
     4921 tatctctgga atgaatccac agcacgcgat cgtagatggc ttaggacctc gtcctgctga
     4981 cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga
     5041 ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga
     5101 aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta
     5161 tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt
     5221 agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa
     5281 cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg
     5341 cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga
     5401 accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag
     5461 aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat
     5521 aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc
     5581 ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc
     5641 acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta
     5701 cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg
     5761 tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc
     5821 caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat
     5881 tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc
     5941 tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg
     6001 tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg
     6061 cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg
     6121 caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa
     6181 taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat
     6241 cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata
     6301 gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa
     6361 attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt
     6421 tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa
     6481 tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta
     6541 tacataacct tgatacttcg accacttata tatcgaacac acaaatacct atccattgca
     6601 ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt
     6661 actcttagta aagtatctga actattaatt tttaaactat catttttatc ctcaactaca
     6721 tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga
     6781 attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat
     6841 tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt
     6901 aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat
     6961 ataccctata aaagctaata agatggttag gaatccttta gaatttttac tcattcatag
     7021 acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag
     7081 tgactcaaaa taaaaaaaac cttacattat actggaatga actgtattga tagtgtacct
     7141 aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga
     7201 tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct
     7261 ttgagattct actttggttc actatctgac ccaattgcac attttcgatc attacaagta
     7321 ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa
     7381 tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat
     7441 gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga
     7501 tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa
     7561 gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt
     7621 attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca
     7681 aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt
     7741 tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact
     7801 tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa
     7861 tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt
     7921 ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac
     7981 ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt
     8041 ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac
     8101 gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca
     8161 tacaatatca ccttttttag gaatataaat tctttcttct gacactataa aatttctctc
     8221 ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca
     8281 ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg
     8341 atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca
     8401 gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta
     8461 tactatataa cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt
     8521 gtttaaagta tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct
     8581 ttaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac
     8641 tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca
     8701 aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc
     8761 tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt
     8821 cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa
     8881 gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat
     8941 gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat
     9001 acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa
     9061 tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga
     9121 agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc
     9181 atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc
     9241 tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg
     9301 agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt
     9361 caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat
     9421 tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac
     9481 tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa
     9541 acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca
     9601 cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc
     9661 ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact
     9721 tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa
     9781 ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc
     9841 aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt
     9901 tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg
     9961 ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt
    10021 gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa
    10081 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa
    10141 atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
    10201 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta
    10261 tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc actaaacggt
    10321 gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    10381 gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt
    10441 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat
    10501 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt
    10561 aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta
    10621 gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg
    10681 ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat
    10741 tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt
    10801 catccactgt actcaactca ataatcaaat tacctacagg acagccatga taagtgatct
    10861 gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt
    10921 ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga
    10981 tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg
    11041 tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa
    11101 gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt
    11161 tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa
    11221 gactgtgcca aattatctga cacagccctc actataaaac tgttctcata tagctagatg
    11281 ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa
    11341 tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc
    11401 cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact
    11461 ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat
    11521 tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa
    11581 gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat
    11641 aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg
    11701 ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta
    11761 aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc
    11821 aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc
    11881 gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt
    11941 attgaacatg gtggattctc cattcgttat ttattttaat acatcattta gagcttcact
    12001 cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat
    12061 agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag
    12121 aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac
    12181 ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc
    12241 tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta
    12301 aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat
    12361 gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat
    12421 cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc
    12481 tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt
    12541 tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac
    12601 ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat
    12661 tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag
    12721 aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc
    12781 aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg
    12841 atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa
    12901 aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc
    12961 accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac
    13021 tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa
    13081 tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa
    13141 tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt
    13201 ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc
    13261 ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt
    13321 ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc caaatcatct
    13381 gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc
    13441 gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt
    13501 tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga
    13561 tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca tctaaattat
    13621 aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat
    13681 gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg
    13741 ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct
    13801 cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg
    13861 tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa
    13921 ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt
    13981 ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata
    14041 gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag
    14101 ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc
    14161 ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc
    14221 aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg
    14281 tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc
    14341 tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc
    14401 gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc
    14461 tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg
    14521 ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt
    14581 gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt
    14641 tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct
    14701 tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat
    14761 tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct
    14821 ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa
    14881 tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact
    14941 gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc
    15001 taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt
    15061 aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt
    15121 caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca
    15181 accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc
    15241 ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat
    15301 tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt
    15361 aaagaatttt attccattat aataataggg aagatggcta gcagtcagca taaccgcagc
    15421 atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc
    15481 gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc
    15541 tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt
    15601 tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc
    15661 tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc
    15721 attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt
    15781 tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta
    15841 tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt
    15901 accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata
    15961 cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc
    16021 tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt
    16081 tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc
    16141 gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct
    16201 tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt
    16261 aatatttctt catattttgg tattttttac ttttattttt tgttttcatt caggttgcta
    16321 acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg
    16381 atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat
    16441 gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat
    16501 ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg
    16561 aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg
    16621 tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt
    16681 ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat
    16741 cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac
    16801 tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt
    16861 tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca
    16921 ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc
    16981 tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag
    17041 tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta
    17101 tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac
    17161 aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc
    17221 gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga
    17281 tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat
    17341 tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc
    17401 aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct
    17461 attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca
    17521 tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt
    17581 gacaacggcc attgctccag caattggccc cacttatgga ggcattatga cttcacattt
    17641 tacatggaat catatcttcc tgtttcttgt gcccgttttg tttctttctt tgattatcgg
    17701 ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt
    17761 tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt
    17821 ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag
    17881 taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt
    17941 gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc
    18001 gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc
    18061 cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg
    18121 tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt
    18181 cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat
    18241 cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga
    18301 actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc
    18361 tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga
    18421 agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt
    18481 aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac
    18541 ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt
    18601 tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac
    18661 ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa
    18721 gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt
    18781 ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa
    18841 acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg
    18901 gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt
    18961 tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc
    19021 agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca
    19081 caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg
    19141 ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg
    19201 gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg
    19261 cagctcttag catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt
    19321 gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat
    19381 gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg attgactaag
    19441 agtacagaac agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt
    19501 gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat
    19561 ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc
    19621 ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag
    19681 gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga
    19741 tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc
    19801 tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga
    19861 gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta
    19921 tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca
    19981 aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca
    20041 ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt
    20101 ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga
    20161 ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg
    20221 tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc
    20281 ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg
    20341 ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg
    20401 tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca
    20461 ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta
    20521 tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca
    20581 tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat
    20641 tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat
    20701 tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc
    20761 gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta
    20821 tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca
    20881 caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc
    20941 ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct
    21001 ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta
    21061 tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg
    21121 ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg
    21181 cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt
    21241 gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc
    21301 ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga
    21361 atactggata acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta
    21421 ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga
    21481 gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac
    21541 cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc
    21601 agccgtttgc taaccagaat gttttagcag catcccatga accataacga gatgttacat
    21661 attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat
    21721 ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt
    21781 tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag
    21841 caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt
    21901 cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg
    21961 aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt
    22021 tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata
    22081 atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa
    22141 acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca
    22201 tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta
    22261 gaaatatttc ataggtaaaa gaaacatgac atttaatg
//
LOCUS       DAZAJZ010000046        22131 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000046 DAZAJZ010000000
VERSION     DAZAJZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.046"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..2031
                     /gene="tdc"
                     /locus_tag="ACG86D_001651"
     CDS             196..2031
                     /gene="tdc"
                     /locus_tag="ACG86D_001651"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN3140790.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2336..3814
                     /locus_tag="ACG86D_001652"
     CDS             2336..3814
                     /locus_tag="ACG86D_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3140791.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3780..5111
                     /locus_tag="ACG86D_001653"
     CDS             3780..5111
                     /locus_tag="ACG86D_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3140792.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5225..7777
                     /locus_tag="ACG86D_001654"
     CDS             5225..7777
                     /locus_tag="ACG86D_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN3140793.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7784..9223)
                     /locus_tag="ACG86D_001655"
     CDS             complement(7784..9223)
                     /locus_tag="ACG86D_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140794.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9198..9380
                     /locus_tag="ACG86D_001656"
     CDS             9198..9380
                     /locus_tag="ACG86D_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140795.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9569..9931
                     /locus_tag="ACG86D_001657"
     CDS             9569..9931
                     /locus_tag="ACG86D_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN3140796.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10118..10975)
                     /locus_tag="ACG86D_001658"
     CDS             complement(10118..10975)
                     /locus_tag="ACG86D_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3140797.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11095..12006
                     /locus_tag="ACG86D_001659"
     CDS             11095..12006
                     /locus_tag="ACG86D_001659"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN3140798.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12013..13664
                     /gene="mdcA"
                     /locus_tag="ACG86D_001660"
                     /pseudo
     CDS             12013..13664
                     /gene="mdcA"
                     /locus_tag="ACG86D_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13766..14065
                     /locus_tag="ACG86D_001661"
     CDS             13766..14065
                     /locus_tag="ACG86D_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN3140799.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14055..15731
                     /gene="mdcD"
                     /locus_tag="ACG86D_001662"
     CDS             14055..15731
                     /gene="mdcD"
                     /locus_tag="ACG86D_001662"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN3140800.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15735..16328
                     /locus_tag="ACG86D_001663"
     CDS             15735..16328
                     /locus_tag="ACG86D_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN3140801.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16352..17428
                     /locus_tag="ACG86D_001664"
     CDS             16352..17428
                     /locus_tag="ACG86D_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN3140802.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17453..18322
                     /gene="mdcB"
                     /locus_tag="ACG86D_001665"
     CDS             17453..18322
                     /gene="mdcB"
                     /locus_tag="ACG86D_001665"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN3140803.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18433..19197
                     /locus_tag="ACG86D_001666"
     CDS             18433..19197
                     /locus_tag="ACG86D_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN3140804.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19686..21779
                     /locus_tag="ACG86D_001667"
     CDS             19686..21779
                     /locus_tag="ACG86D_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3140805.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21804..22106
                     /locus_tag="ACG86D_001668"
     CDS             21804..22106
                     /locus_tag="ACG86D_001668"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3140806.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7171 a         3784 c         4717 g         6459 t
ORIGIN      
        1 tattgaaaaa ttgttcgctt ttttgcgttg aattatcaga aaaatccgac tttatttatt
       61 tttgtttgta aaaatctacg tggatttgat gttataaaaa aaaaattctc atttttggcg
      121 ctatagtgaa gaggtgttac agataaacac atgtatctgc aagacattga ccaacaaaat
      181 atggagggaa gaaaaatgaa agatatggat atcaaggccg tctttatagg cgacaaagct
      241 gaaaatggac cagtttataa aatgttgttg aacaagatgg tagatgaaca tcttggatgg
      301 agagaaaatt atattccttc agatatgcct gcaattagcg aaggagataa gctcactcca
      361 gattatctag ccacccgcga ccatatgata gaggtcttgg atgaagtcag tcagcgatta
      421 cgcgcaggat cgatcccatg gcattcagca ggccgttact ggggacaaat gaatgctgaa
      481 acattgatgc ctgctttatt agcttacaac tatgcgatgc tgtggaaccc aaataatgtt
      541 gcattggaat cttctatggc aacttcgcaa atggaagcag aagtaggaca ggattttgct
      601 tcattattca atatgacaga cggatgggga cacattgcag cagatggttc tattgctaat
      661 cttgaaggat tatggtacgc ccgttgcatc aaatcgattc ctttagctgt caaggaagtg
      721 ctgcctgaaa aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc tgttgaagaa
      781 attttggaaa tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa agcagcttct
      841 tcccgtagcg gaaaaaatat ccaaagatta gggaaatggc tggtccctca aacaaaacac
      901 tattcttgga tgaaggcgtt agatatttgc ggagtcggct tagatcagat ggttgctatc
      961 ccagttcaag aggattatcg aatggatatt aatgctttgg aaaaaacaat tcgtgaatta
     1021 gctggacaaa aaatcccaat tctaggtgtg gttgctgtcg taggaacgac agaagaaggt
     1081 caagtagaca gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga tgaagggatc
     1141 tatttctatt tacatgttga tgccgcatat ggcggctatg cgcgttcact gtttctaaac
     1201 gaagcaggag aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga acatcatgtt
     1261 ttccaccact gcgtaacgat tgacaaagag gtatatgaag ggttcagagc tatttcagaa
     1321 gcggattctg tcacgataga tcctcataag atgggctatg tcccttatgc tgctggagga
     1381 atcgtaatca aacataaaaa tatgagaaat atcatttctt atttcgcgcc atatgtgttt
     1441 gaaaaatcag taaaggctcc agatatgtta ggagcttata ttttggaagg atcaaaagct
     1501 ggagcaaccg ccgccgcagt ctggacagca catcgtgtat tgccattgaa tgtgaccgga
     1561 tatggtcaat tgattggggc ttcaatcgag gcagctcaaa gattcagaga atttctcgat
     1621 catctgactt ttactgttaa aggaaaaaca atcgaagtct atccattgaa tcatcctgat
     1681 tttaatatgg tcaattgggt attcaaagaa caaggctgta cagatttgaa cgctatcaat
     1741 gaattaaatg aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt ttatggtgaa
     1801 cggtttatta cttctcatac tacatttaca caagaagatt acggtgattc tccgattcgt
     1861 tttgttgaaa gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa aatcactctg
     1921 ctacgtgcgg ctatcatgac tccttatttg aatgatgatc gaattttcaa tttttataca
     1981 aaaaaaatcg ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata gaactgattt
     2041 ttgtgtgaaa gaataacaag agacatatgg agaacagaca attatttgct tcatcaaaac
     2101 aattgtattt ccccctgttt ttcatatatt cctcgccaag atattctttt ttataaaacg
     2161 ttctataagt tggatcgttc gtttcatgta tcaaacccag ttcactaaga tcgttcaatg
     2221 gatgtttcgg tgatactggg ttttgtactg aatcgatgaa accttgtcat ttctgttctt
     2281 gaacaaaaat aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa taaagatgaa
     2341 agatactggt tcatcaaagc aaatcacgac catgaccttt atcggaatga cctgcgccct
     2401 tgtagcaagt gtacgaaata ttccagatgt agcagcaacg agctggacga tgatttttta
     2461 catgctggtt gcagtattat tgtacgcttt tccaatctca ttgatctctg gtgaatttgc
     2521 tggaatgttt ccccaaaaag gtggtccaga actttgggta tcgaatgctc taggaaaaaa
     2581 atgggggttc gttgtctcat ggctgttatg ggtccaaatg tttcctggga tggtgatggt
     2641 cgcttcagca ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc ttggtttgaa
     2701 cagcaaattt acattagttg tcattttagt cgtttattgg atcatcactt ttttgaattt
     2761 gaaatttgat atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat tgtacgtgcc
     2821 tttgacgatg atgctcttat tagggtttgc tgcgtggatc aaagttggga tcgtaccaac
     2881 cggaacatta ggcagttttt catgggacaa attaatacca gatactacga ctgcatcctc
     2941 atttgtttat tttgcgccga tcatgtttat ttttactggt atcgaaatgt cctctgtgta
     3001 tatcactcga ttggaaaatc cagttaaaac gtatattcgc ggagtatttg ccgcgttaat
     3061 ttttatattt ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg taccaaaagg
     3121 acaaatggaa ttgaataata tcgcgcaatc tatttcgatt tattgccaaa ttcttggttt
     3181 gccacatctt attgtgaatc tgtttagttt acttgtcttc attggtgtag cagtccaatt
     3241 atctgcttgg gcctcaggac cagcaaagac agtgacagaa agtgctagaa aaggtgcgta
     3301 tccaccaaaa ttcaattttt ggaagacgaa tcaatttgac gtatccaaat ccgttatttt
     3361 gactcagtct gtcattatct caatctttgc acttttttat ttgctgattc ctggcgtcaa
     3421 tcaagcattt ttgatgctcg taaattcaac tactgttatc tactgtatcg tctatgtcat
     3481 catggggatc gcagttttga gattgcgtta tacacatgca aagttgaatc gaccatttag
     3541 aattggaaaa aaagagaaaa agagcaatct tggagtttgg actgtcgtta ttgttttatt
     3601 tgcggcaatc ggattttctg tcggactgac gatgaaagct ggaacatgga tcaatctaat
     3661 tgcagtcaca gccatcagtg tcattttgtt tgtagtacca ctctgtattg aaaaaataaa
     3721 gaaaccttct tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag gtgaacttga
     3781 tggaaaaacc ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta atcgcattag
     3841 gtggcgcaat tggaacagga ttatttttag gttcagcaaa ggcaattgca tcagcagggc
     3901 cagctgttat tctcgtttat ttgatatgcg gtttggctat ttatggtttg atgcgggcca
     3961 tgggtgattt atttttagca aatagtacat ttaactctat cagtgatttc gttgactatt
     4021 atttaggcaa aaagtgggca ttttttgtca attggactta ttggctttgc tggcttggag
     4081 ctggacttgc ggaattaaca gcaataggat tgtatatcca tttctggttt gtacatgtac
     4141 cagctttgat aagcggaggt agcgcactaa tgatcttaat ggcagtcaac cttgttgcag
     4201 tcaagtggtt tggtgaattg gaatcttttt tctcaatcat caagatcttg gggattttcc
     4261 tttttatctt attgggtata ggattgagtg tctcagattt tcatacccag tctatcggaa
     4321 tcaaagaaaa ttttggacag atcgatgaat tactaccgtt tggctttgct ggaatggtag
     4381 tctcatttcc tatggtttta ttcagttttg ctggaattga aatgatcggc ttaaccgttg
     4441 gcgagacaag tgatccgcaa aagaatttga aaaaagcaat caactgtctg ccttggcgta
     4501 ttctcttttt ctatatagga acgattttgt cgattttatt tgttgttcca tggcattgtt
     4561 tagatttaaa agaaagtcct ttagtgacta tgctgcaagt gatccattgc caaggcggag
     4621 cagcaattgt caatctaatg attttggttg cttctttgtc ctcagcaaat agtgcagtct
     4681 acagtactag caggatttta tatcaaacag caaaagaaaa aagtttgccc cattggtttt
     4741 gtgcgctttc taagaaaggc gtacctttac acggtatttt aattacagga atgttatttt
     4801 ttgccggtct ttgtctacat ttttttgtag ctgatagtcg attgtttttt cagcttttgg
     4861 caggcatgac aacgagttgt tttctatttg tgtggtcttc gatcttgtgt gcacatgttc
     4921 aatttattaa agaaaagaaa cggaacacta cacaaagata taaagatctt gctttgttgg
     4981 tgttttttac ttcaattgct ttcagcttgt tattcgaaag cacgacacgc tttattcctt
     5041 ttttattggt tatctggttt gtattcttaa attttgtata taaaagaata agcaatagaa
     5101 atatcagtta ggcccagata cattttgcaa aacgaattat ttcagacccc cctgaaataa
     5161 tcgatacgcc tgaaaaaact tccctgcgta taataacagt atcaattaaa caggagggga
     5221 taggatgaac tggtacagat tgacggtaga agatgtattg aaaaactatc aaacatcaac
     5281 agaaggcttg tcgcaagaag aacgacagaa acgcctgact gatcaaggat ataatgaaat
     5341 aaaagcaagg cagcagacaa aacgatggaa aaaaatagca aagcatttta ctgatctgct
     5401 gatgatcgtg ttgatcattg cctctttttt gaaatttgct tctagtgaat atattgaagg
     5461 aagtattatt ttattcgtag tgatcgtcaa tggactcgtt agttactggc aagaaagaaa
     5521 agcggaagaa tcattgaatg gtttgaagca gctgatggga caagaagcga tagtactatc
     5581 aaacgggatg caagaaaaaa tccctgcgaa agaactggta aaaggagatg tcattgtctt
     5641 acgtgccgga gatgtcgttc cagcagatat ccgattaatc gaagcacatg atttattagt
     5701 ggaagaatcg attttgacgg gagaatcaga agcatctgaa aaaagtcatg ccgttttgac
     5761 agaagaagaa tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc ttgtacaatc
     5821 tggttctgca gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg gtaaaatcaa
     5881 tcaagcattg caatcggtcg aacaacaaac gacgcctctg attaaaaaaa tcaaccgatt
     5941 aaacaaacaa atttttcaag gcattctttg tttgagccta ttcttagtta tttttacgac
     6001 atttcgatat ggtatggatt ggcatatcct tctttcggct acgattgctt tggtggtgtc
     6061 aatggtaccc gaaggattgc cagctgtttt gaccatgatt ctatccgttg gagtacatga
     6121 aatggcaaaa gaaaaggcga tcatcaaagg acttccttcg gtagaaacat taggttctat
     6181 gacagtgatc tgttcagata agacaggaac attgacaaaa aatgaaatga cagttgtgga
     6241 tgtagctgca aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc aggaattaaa
     6301 aacaaaagaa gaacagaaaa cagaaaattt aagtggcaat ccgacagaag ttgcgctgat
     6361 tcgacacaca gaaacagcca atctaccttt gaaaaaaata gaagcaaaga ttccgttcag
     6421 ttctgaatac aaatatatgg caacgatgca tgcggaggaa aaaggagcgg tgatttacgt
     6481 caaaggtgca ccagaagtat tatttgctaa atcaactctt tcttctgcag aacaagaagc
     6541 atggagtcag acagctgcag aatttgcgag aaaaggtcag cgtgttttag gatttgctta
     6601 taaaaaagta gattcgaaac aggaactcac gcatgagact ttaacccaat taacttttgc
     6661 agggatcgcc ggattgatcg atccgccaaa agaaagtgca gtcaaagctg taaaggaatg
     6721 ccaacaagca gggatctcag tgaaaatgat tacaggggat cacaaagaca cagcaaaagc
     6781 gattgcggaa caaattggct taaaacatac tgccaaagtt ttagaaggga tcgatttgga
     6841 tcttatgtct gatgaagagt tgattcagca agtgccaata gtcgatgtct ttgctcgaac
     6901 gacaccagaa cataaactgc gaattgttaa agctttgcag aaaaatggag aaatcgtcgg
     6961 aatgaccgga gacggcgtaa atgatgcacc tgctttgaaa agatccgatg taggaattgc
     7021 tatgggaatc aaaggtagtg aagtaagcaa acaggcagct gatatggttt taggagatga
     7081 taatttccat acgattgcaa aagccgtaaa agagggccgg cggattttgg ataatttgca
     7141 aaaaaccatc aatttcttct tgccaactgc attagctcaa ggattgatct tgatttgggc
     7201 acttatgctg aatcgcccgc tacctttgtc cccagtacag attttatggg tcaatatggt
     7261 aacgacaatc actttatctt atgcactagg gttcgagtcg ccagatccag aaatcatgaa
     7321 gcgcccacca agagatccaa agcaaggaat cctatctggt tatcatttgt ttcggatcat
     7381 ctatgtctcc ctactgatca tggttcctgc ctatttattg gctatgcagt ttgaagggca
     7441 aagcttacag caaaccattc ttctgcaaaa tatcgtctta gctcaagctg tttacatgat
     7501 caattgcaga gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa acaaagcgct
     7561 ttttctttca ttaggtatcc ttgctttatt gcaagggatg gttatttttc taccagtcgg
     7621 ccagcagctg atcggtacag taagtttaac gatgacacaa cagttattga ttggagcaaa
     7681 tgtgattctt ctattcttag ttgtagaaat agaaaaaggg atcatgaaca aactgtttcg
     7741 caaaaaagaa aaaacagcta gagaatacag tcgctagccg ttactataaa aaagttttga
     7801 aagtgagttc agtcaaacga ttgagctcat ttttcgttgg aatagaagaa atgtatacaa
     7861 tagggatatc tagagaatct agagggaaat tggaaatcaa gatatctgtt ttttcaaaag
     7921 aaatagtgga aagatcggtt agttcgatag gtaagaagca gacacgtttt cctaagtaat
     7981 cttttaattc ctgctgcaag aacgccttcc atgaaggttc tccttggaaa ataaaataga
     8041 cattgaacaa attcggtttg atagaaaaaa tcgtttgttg gatcagtaga gacaagagtt
     8101 ggagaaagta atttggttca ttgatttcta tttctttttt tagatcgttc agtaaattct
     8161 ctttctgttg atacaagtgt gcatattgct gatcatatgt ttctaaaaaa cgatggtatt
     8221 gacggtccaa tgcttcaatc aatatcggcg attcatgata ttttaatatg aggagagcca
     8281 agttatcagt cagagatgtt ttttttagtt gatcaagaga cagctctgaa gctagttcct
     8341 ctacaaacgt ttcgaggacc tctaaaaatg gagaatgatg caggcagcga gcggtttctt
     8401 gtccatagtt attgttgtaa ttccaacttt ccagaaagca gaaagaagca aaagaaagct
     8461 cagtgtcttt aagataaata ccttccgtta aatacaattt tttcaatgca gagtcataca
     8521 gggaggagag tgtgctgttg gtatggatag aagaatctaa tcggcaattc gcttttatac
     8581 ggatcgtatt gataaagaac caacatgttc cgaaaatctc ttctgtttca accgctagtt
     8641 cagtttgact gaggtttctt aaaaattgaa aataatggga ttcatggatc gaataatcct
     8701 cgaaaaaata gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa gtacgaatcg
     8761 cacgctcttt tccaagaata caagctgtat atttcgtcaa atcgatctgc aagtcgtaac
     8821 ttgctaaacg ctgattgatt ttagtcacat gtcttttcaa tgtcccataa gaaagcccat
     8881 ggtcggctag aaaatcaggg acagcaactt tttttgtaaa aagcagattt ttcagcagtt
     8941 cgacactgac tgattgtttg aaaaaaatct cccaaaggtc atttgttaat aaattttgct
     9001 ggttttgcaa actgattcct gcttgatcgc tgatgatgtc ccagccttcg ggcagctgag
     9061 agcgaataga ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg atttcagcta
     9121 attcatttac ggttatcttt gtatgattca gcaattgttc tactaaagaa acatgacgtc
     9181 gaatatcttt ttcggtaatg atttttttca ttagtgaata catcaatcag actcccttct
     9241 attctaagaa tacggagaaa ataaacggtg gtcaagtttc aactgatttt tcaatcattg
     9301 tttatttttc tggagcattt actgatgata agaatttcat catgaacttg aaaaatgata
     9361 ccactaaaat caaaaaatag gtgggcaaac aataataaac attttaatag tagatggtag
     9421 caagaccaac acaggcggat tgaacattta agagttgata aaaaagaaaa atatcgaagt
     9481 atttgattag caactacttg tattcatttt tctattctta cgtggtatct ttgaataaag
     9541 cgaatcaata agaaagagat ggaagcagat gctaacgaaa aaagaactaa tcgatcacat
     9601 caatgaaaca tacaatttgt ctccagaata tatttttaaa aaatttcctg attattgcgt
     9661 attcagacat caaaaaagca aaaaatggtt tggggtcctg atgtctgttc cagaagaaaa
     9721 aatctatgat gatgcaagtg accataggat catcaatatt cttgatttga aggtcgatcc
     9781 tgaacttggc ggaattttgc gaggaaaaga ggggtatttt ccagcctatc acatgaataa
     9841 agaacattgg atctctattg atctacgtaa aatagaaaag ctagatgaac tagcagattt
     9901 gattgatgga agttttaaat caacgcaatg aatgtctagg acaatagaga agaggttgtg
     9961 acagaaatgt ttagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt
    10021 tgggaaattt cggcttattt tctaagaaat tacttctgga acaccgtttg ttcgaaaaaa
    10081 gagctgcgac aagaattttg tcccagctct ttttttttta ttttcctaaa taaaattccc
    10141 gcatcgtttt tgcaatcatt ttgatcaaag gatgatcttc tgtgaagcta gaagataaat
    10201 aaattgttcg tgtcaagggc aaatctttga tttcccgcaa gacgaaagga tcatttttat
    10261 cgtaagacca agaaactttt gggaaaaagc taacagctaa tccttgcttg acaatagaac
    10321 gtaatgtcgc aggatcatct gtcgtcgaac tgatcgtcaa ttcgatactt ttcgcttcaa
    10381 gtgctgcatc cagtgtatga cggaaggcgt tgctctttga cagcatcaag atgggggtgc
    10441 gttggatatc ttctaattgc agatgttctt ttttagcaag aggatgtttc ttaggaatgg
    10501 cgacataaac aggttctttc atgataggaa tattggtcat gccaggcttc atctccgaag
    10561 tgatgtacag tacaggatcg gtaatatcat ctggtaaatg atgttgcatg atcgttaatt
    10621 ggatatcagg atgcttttcc ataaaaattc ggaccaagtt cggaatagaa acagaggcta
    10681 cctctaaata caatatgacg gaagtaacga tcttctcatt gaccgaagac aataatttga
    10741 ttccttcttc taatgtgtct aatgtcttga ctgcatatgg ataaaattct tctgcataaa
    10801 cggtcgtctc aatgtttctg ccgctatggt aaaacaaagg gacacctaat tcgttttcca
    10861 aatgtttgat cgcacggctc aaggcaggtt gagaaataaa taattctttg gctgcttggg
    10921 taacattttt tgtttcgaat acttttttaa aatactgcag atgaagcaat ttcataatta
    10981 tccatcccat ccataccttt ttgttatggt tttttctttt tttggcattt tttattatag
    11041 cagaaattga ctaagatagc agtggaaaaa gtaagtgctg aggaggcgag gctcatgagt
    11101 acaggtttct tatttcctgg acaaggcagt cagaagttag ggatgttagc ggatatccct
    11161 gaaatgtaca tggaacgttt agaaaatgtg acggggtatc ggttagttga taaagaagaa
    11221 aactatcaag atacggtttt tatccagcta gcattactga caaaagcagt attttattta
    11281 gatgaaatgg aaaaaaacgg tattcagcct gatattgcag caggacattc gattggtgct
    11341 ttttgtgcag ctgttgcttg tcgatctttg agatttgaga atgcagcgga actagtctat
    11401 caccgcgcag tcttaatgaa agaagcttat ccaagcggtt atgcaatggg cgtagtagta
    11461 ggtgtgacga gaagcgaagc tgaggaaatt gtagcaaaaa catttgatcc tgaccatcca
    11521 gtttatctgt caaatgaaaa ttgtccgatg caacatacgt tatctgggag tatcgaaggt
    11581 ttatccgtta cgttggcaga agccaaacgg caccaagcgc aaacagcgaa gctgctgaaa
    11641 gtccctgtgc catctcactg tctcttgatg aatgatacgg tcaagaaatt caagccgtat
    11701 atgcagtctg tccatctatc tgatgctaga tgtttgtata taaaaaatac ggatggaaga
    11761 gccactcagg acgcagaaga gattcgtaaa gatttgttgg aaaacctagc tttccctgtg
    11821 cagtggaacc aaatgatgga cttggcaaaa gaattaggaa tggatctgac gattgaattt
    11881 ccccccggca atactttgac aaagctgatt catacaaaat ttggtgtaga taaagggatc
    11941 cgtacgatca atctagacca acatggtatt gatgacgcca cgtttctata tcaaaaatgg
    12001 agatgactaa ttatggaaaa accaaaccgc aactgggctt ctaaacgcga aaaaaaagcg
    12061 cagcgattgg aaaaaatcag caacttgctc aatggcaagt atgtagaaac tgaaaaaatc
    12121 gtagaagtaa tggaaaaact gattgctccc ggcgacaaag ttgtattaga aggcgacaat
    12181 caaaaacaag caagttttct ttcagaaagt cttgaaaaag tagatgcaga aaaagtgaat
    12241 gatctgcatt tgatcatgtc tagtatctca cgaccagaac atttgaatat ttttgaaaaa
    12301 ggaatcgcaa gcaagatcga tttctcttat gctggtgcac aaagcttgcg tgtcgcccaa
    12361 atgattgaag acggcacaat gaaattaggt gatatccata cttatttgga actttatgga
    12421 cgcttattca tcgatttgat tccaaatgtt gttttagtag ctgcagataa agcagataaa
    12481 gacggcaact tgtacacagg tttcaatacg gaagaaacac caacaatcat cgaagctgca
    12541 gctttcaaag atggtatcgt gatcgttcaa gttaacgaag ttgtcgattc tttaccacgt
    12601 gtagatatcc ctggtgactg ggtagacgtt gtcgtgaaag cagataaacc ttatcaatta
    12661 gaagctttat tcacacgaga tcctcaaaac atcacagaat tgcaaattct aatggcaatg
    12721 atggcaatca aaggaatcta cgcagaacac ggcgtacaat ctttgaatca cggtattgga
    12781 ttcaatacag cagctatcga attgttactg ccaacttatg gtgaatcact agggttaaaa
    12841 gggaaaatcg cgaagaactg ggtattgaac ccacatccaa cgatgattcc agccatcgaa
    12901 tcaggttggg ttgaaagcat ccatagtttt ggtggcgaag ttggtatgga aaaatatatt
    12961 gctgctcgcc cagatgtctt ttttgtcggt aaagacggca cgatgcgcag taaccgaaca
    13021 ttatcacaag ctgcaggaca atatgccatc gatatgttta tcggttcaac cttgcaatta
    13081 gattacgaag gaaactcttc taccgtaaca aaaggacgtt tatctggttt tggcggtgcg
    13141 cctaacatgg gacataatcc tggcggtcgt cgtcattcta caccagcatg gcaatcactt
    13201 cgcgacaatg atgatccatt agggaaaggc caaaaattag ttgttcaaat ggttgaaaca
    13261 tatggttcaa ataaaaaacc agtctttgtc gaagaattag atgcactagc tgttcaaaaa
    13321 caagcaggct tagccaatgc aatgcaccag tgatgattta tagcgaagat actacacata
    13381 tcgtgacaga agaaggaatt gcttatttgt acaaagcaaa aacaaaagaa gaacgacaag
    13441 cagcaattgc agcaattggc ggtgttacac ctcttggaat gactagcacg aaagaatcac
    13501 tagatagctt gcgtgcagcc ggcatcgtga aattaccaga agatctaggc atcaaacgta
    13561 gtgaagcgaa acgttcatta ttagctgcac aaaccatcga tgatttgatc gaatggtcag
    13621 atggcctgta tgaaccacca atgaaattca gaacttggtc ataaaattgg cgagaaaaac
    13681 cttttgtcgc catttaccca gatggaagaa gaaagacgaa taagcagaac gtgtatgctc
    13741 tgttttacaa aaaggggaga gatccatgga acaattagct tataaatatc caacgaaaca
    13801 gccgatcaat aagaaaatcc atgtaggagt ggtcggttca ggagatttgg aaatcttgat
    13861 gtttccaaca gaaaaaacag aatctactgt aaaaatttgt acaggaagcg atggcttcgg
    13921 cgaagtatgg aacaatgtat taacgagatt ttttgatcgt tatccaattt cagcagatat
    13981 cgtgatcaat gacttcggtg ctacaccagg agttgtgtac ctacgattga cgcaagcaat
    14041 ggaggaactt tcagatgaaa aatagttttg tagaattaaa cgccagagaa cgagtgaacg
    14101 ctctacttga caatggacgt gaattgattg atccattcga tcaaatcatc gcgccaagtt
    14161 taccagcaca aggaatcgtc cctgaaagtg atgacgggat cgttgtcagc cgtggcacga
    14221 tcaatggcaa acaagcagta gtaatcgcta tggaaggaaa attccaaggt ggcggtatcg
    14281 gtgaagtttc cggtgcaaaa atcgttgctg cacttgaaca tgtgttagaa gaaaataaaa
    14341 aaggaaatga gatctttcca gtcattgtac tagatacagg tggtgttcgt ttgcaagaag
    14401 caaactacgg actgttatct atttcagaaa tcggaaatgt aatcattgct ctaaaacaat
    14461 atgtaccagt catcggtttg attccaggac gtgtaggatc atttggcggt atgtcgatta
    14521 cttcagcatt aatgagttat ttaatcgcaa caaaaaaagc gagagtcggt ttgaatggac
    14581 cagaagttat tgaacaagaa gcaggcgttc gagaatttga ttctagtgac aaagacttga
    14641 tttggaatac attaggatct cgtcaacggg tagaatcaaa aatcattgat gaattagtga
    14701 ctgatagtgt agaagcaatc aaagctgctg tcatcgcagc aatggacgaa cgaaaagaca
    14761 cgcatcgcag tgaaagctca gcattttatt tatcattact tgattcttta gatctttcaa
    14821 aaccaatggc cattgacgat tacaatgaag caattgctaa ccatcaagca aaatcaattg
    14881 acttaccaga agtagcagaa ggaaatgaac cagctgcagc aagtgttggt tacgattggt
    14941 ttgcggcact aactggcatg aaaaatccaa cttcttctat tagtactgtg tattatggta
    15001 aatcaagtaa attcatagaa gaagccatcg tgacatcaat cgtacctgat cctaaaaacc
    15061 cgatgtaccg tgtacgaaac ggtgaagttg gtttagttga aggtttccgc atgggacaaa
    15121 ttttggatca cgtttacgaa gaagataaag acaaaaaagt gaaacggcca atcatcgttg
    15181 tgatcgatgt accaagccaa gcatacggct ataacgaaga attgatcggt atccatgtgg
    15241 ctttggctaa tagtgctgct gcttatgcaa aactccgtca agctggacat ccagttattg
    15301 gattgattgc aggaaatgct atttcaggtg catttttagc acatggatta caatcaagcc
    15361 gcttgattgc tttaaacaat aaagaaatta ccgtccaagc gatgtctaaa gaaagtgctg
    15421 cacgtgtgac aaaacgttcg gttgctgaaa tcgaagcagc tgctgaaaaa gttccagcta
    15481 tcgcttacga tatcaataat tatacaaaac taggagcgat ctatcatttc ctagaagata
    15541 tcaccgacca agctgcttca gaacaaaatg tcagcaaagt catcgaagca atcaatgaag
    15601 ccattcaaga tgtccgcaat acaaatgaca cgatgctgac aaatcgatac acaaacgaaa
    15661 cagccaaaac attcggacgt gtcgaaacaa ataaagtccg tgccaaaatg gacgaagaat
    15721 gggatgcata aaaaatgatt caagcacatg atattgtcct ttttgataaa catgaaatag
    15781 aagggttcac cttgcctgaa tgggtcgttc taactgaaaa gatgttcgga acagttcgtc
    15841 gtgcgtcgac ggatcacgca gaaatactac cgattggttt acgtggacct gcccgcaatc
    15901 agagatggtc aacagaaatc tcaaaacatg ccgtcaaaca agtcattcat ccgtgggaca
    15961 tcatcgaaca agaatcgtat agacaagaga aaatcatgga ttatcctgtt tatcagcaat
    16021 ttcatgctgc tagaaacata ttatcaggat gtaaatgggg aatcggcggc agcttaggat
    16081 ttgaattatc gactgggatt ccggcagtaa aagaaaccag tgattttgat ttgctgctgt
    16141 acgcaaattc accgattgaa ttgccaattc aggtaattca atcgcatcct gctttttttg
    16201 agcaattcga tacccaagta gtgactcggc aaggcggttt ttcactaaaa gagtatttga
    16261 gagagccaga gaaaaaaatt ctattgaaaa cagtcgaagg acctaaatta accaaagaac
    16321 tttggtaata aagaaaaaga gagagaaaga gatgtttgaa aacaaaaaaa tcaaaatcaa
    16381 cgtaagtgac ttccagaaag tatcagctgt aacggctgtt atgatgcaaa cgatattgag
    16441 ctttgctttg gcaaacagcc acagcgcaac aaatacagct tttcttggta ccttttacgt
    16501 catcgttaaa tacacagcac caatgtttat ttttgcaatt gtttataata tggtaaaaac
    16561 aagtcagcat ttgtcttatt gggaattttt gaaagaaaag ttttttgaac tagtcgttcc
    16621 gtatcttcta tggacgacgg cttatctttg gctatttcca gcagtccaac aacaaactcc
    16681 gtatacggat actgcaagct ttttactgaa atatgtcaca ggtgatggag cgcctcattt
    16741 atggtatacc gttatgatgc tgcagatcca attgttcatg ccgttttttg tttggctcgg
    16801 ttacaaagta tttgctaata aaaaatatgt atggccagta ttgattggtt caacgatttt
    16861 atatgtcgca tggtatctgt tctatcaaac acaagtattg actgggcctt atcatgacag
    16921 ctggtactta ttagatcggt ttgttgctag tttcatgatt tatggggtag ccgcatttgt
    16981 ttatcacgaa aaagtctatc aatatttgga cagagtacgt tatcttttct tacctgttgc
    17041 cttagttatc gcgttctttt ctgtccgttc attgctcgca catccaggag acttgagctt
    17101 tgcgaatgca ccttacttga atacgatcca aagtttatac agtttagtca tcatctttgc
    17161 tgtcttcata ggcgcttcca aaatgattgt aaacgattcg ccaaaattgc cattgttcaa
    17221 atggttgtct gtttatgctt atcgtacgta tttagccaat gtatttgttt tccaagtact
    17281 gttattattg tttaaagata gttggctgca gttaccttca ggagtcatga ttcttgtagc
    17341 ttatctaatg acggcaagtt gtgcatttgc tttaagctgg ttacttcata tcatttgggt
    17401 agctatcaaa aaaggtttca gcaagtaata gtaggaagaa ggagtgagtg tcatgataaa
    17461 tgacgaacga aaaaaagcag tgactcagca actcgctgcg ttggtagagg aagcattgat
    17521 cgcagaagta acattaagtc caaaacctgg attagtggat gcactggatg cgggtgcaca
    17581 ttccgatatg gactatgctt tatttcttaa gtctgctaag acgctaactc ctttttttga
    17641 agaaatggct caaatagcat ggcatcatgc tattgatcag gagttaagag aaagaattgc
    17701 tgagattggg cgaaaagcgg aaaaggcaat gctgattgct acaaatggca tcaataccca
    17761 taaaggagcg atttgggcaa tggggctatt gatcagtgtc attgcacaaa aaatcagtat
    17821 ggaacagcct ttttctttat cgaaaatact tgatgatgtc agcaagcttg ctgcttttcc
    17881 tgatacaaaa tatcgtcata aaaaaaccac tcatggtcat agggtgaagc aaaaatatgg
    17941 cgtaaatggt gcatatgaag aagcgatatt agggtatccg catatcaggc ttgcaattca
    18001 agcagctaag gagctagata atgattcata tgtacagaaa caaatgcata tgttgttagt
    18061 tttgatgagt tcgctagatg acacatgcgt attgcatcgc agtgatcaga aaacgttagt
    18121 ggaaatgcaa aaacttgctc atgctgctag tcaaagtgct ttaccaaatc gtccatttac
    18181 agcactgttg cacttttgtc aggaaaacca aatctctcct ggaggaagcg cagatctctt
    18241 gtctgcaagc ttgttcatgc tgaagataga ggaattatgg actgccgaca catacatttg
    18301 tccgatgtta ctcaatgact aaataagaca aaattgctaa aaaaacagtt tatgcatctg
    18361 gttattgact cttcctttta gcaaggctat cataaaaata gttttttgta ataaaagata
    18421 acgaggaaga caatgaaaaa aatcaattat ttagcaatca ttttgttagt gggcctatgt
    18481 ttgtttgctc tgccgaccca aggattcgct gtcgcgggtg gtcatggtgg aggtacatct
    18541 agcggtcgaa gttcgggcag aggttcattt ggtggtcgtt catatggggg cagttatttt
    18601 ggcagtcgtt cttacggcag agattcttat gggtattatg gtccaggtac aggtccttct
    18661 agttatgagc aaacgggatt tcttgcatta gctgttttgt cagtaggagg ggtttctgct
    18721 ttttctatcg tcaagaataa aagacaaaag aaaaggctga aagcggagat attgtcccgc
    18781 atgtcaggaa cggctaaaga aaagaaagca cagtttctgg aaatccaaaa gatattcatg
    18841 aagatccaat ccgcttggga agaagaaact cctgaaaaag ccagaagctg ttatacagac
    18901 aagctgtttg ccgaacatca gaaagttctg caaaaaaaca aagcagacgg tgtaagaaat
    18961 catactaaaa aaataactat tcaggaagta ggaaattatc gacagatcca cgaaaatagt
    19021 tttgccatcc gaattgattt cagatgctgt gattattcag tcgatcgaaa agatgggcga
    19081 ttgatctcag gaagtaagac acaacgacag tatttttctc agatatggta ttttgatttt
    19141 tctgagaaag aaaaaagatg gcaagccgat tttattcagc cgattatgct ggattgacag
    19201 gaaatattta tgaacggagt tgtgacaaaa gccttgtcgc agctcctttt tctgaataaa
    19261 cggtgtgcca gagatagcct atcggcaata aggcacgatt ttgtaaaata tgggaagcga
    19321 tttttcaaaa tcgcttctta tcgctcaagg ctgaacatct ctgtcacaac ctctccacta
    19381 aatggtgttt aaaagcttgc atctgcgata ataagaacaa ccaaacgaaa aataggaagc
    19441 actattttag tttagttgtt cttattactt gatgcaggac agctttttca caacctcttt
    19501 tttgtataga tgggcaaaat ttttgtaata ataggcgtat gtctttttcg ttgaggagat
    19561 gaaaaagcat atggcttatt ttttgtttgc tttgcttact ttctttccac ttcaagtggc
    19621 agaaatatct actttttatt atgtaagcgg aaccatatac ttgaaatcag aaggagatca
    19681 tttctatgaa aacaagaaca attgaaatac ttcacagaat catgacagcc caatatccag
    19741 taaaagcaaa acatctagca agtgagttca atgtcagtca gcggacgatc cgtcaggaag
    19801 ttcttgaagc gaatatctgg ttgagcagta aaaagcttcc ggagatccgt acaattcgca
    19861 acaaaggttt tttgttgaag ctggaaaagg aaaagcaagc tcgattagaa actgaaataa
    19921 aagatgtgaa agacggcttt ttgaatcggc gcgaacgtat ttttgatcta gttttagccg
    19981 ttgcttacag ccgaatcccg atccatctaa acaaaaaaga agaagaattt caaatctcta
    20041 aaagtacatt agatgaagat atgagacggt tacgagcaac actggtcagt tatgggatag
    20101 agatcgtcag ttatggaaag caaggactgg tttataaagg ttcagaacga tctatccgta
    20161 cgatgatcta tgattttatc aatcagaatc tgggaatgac cgacttcttt atgaatcaag
    20221 atgcacaatt aacaccggct caaaaaatct ttctccatta ttttcctttg gtggaaatca
    20281 aaaaaataca ggagatctat tccaataagt tgttgaaaca agaagatgat atttataaaa
    20341 aacaaatcct gctttttaca ttgatctgga tccagcgaat caggaaacat gaatttatct
    20401 ctgccattaa ttggaaaaat acagaaataa aagacagtag cttttctgca tttattgacc
    20461 acatgatcga ggaatttcaa ttggacaatg tcccgcaagt agaaagaaat tatcttcgtt
    20521 tcactattga gacgttcaat gctagagata tctccaattc aatcgagtgg gtacaagctc
    20581 aattactaac gatccaattg atccaatttg tagagaacga gacacacgta cctttccatc
    20641 taaaggaaga agctttattc gaaaatctct ataagcacat ggctgcactg attgtaagaa
    20701 tcaagaatca tatccaagtc atgaatccat tgaaagaaaa catccgaaca aattatttcc
    20761 caatctaccg cgcagtcact cattttgttc ctacgatcga agaagtaatt ggtggcaaga
    20821 tactggagga cgagatagct tttcttgtca tccacttttc gacgatcgcc agtgtaatca
    20881 agcgagactt gaattatgtt tataaatcag tcgttgtctg taaccatggg atggctactg
    20941 gaaatctgtt agcagagaat ctgaaagaaa agtttcctca gatcgagatt actgcagttt
    21001 taagttcaaa agaaatcgaa ttagtagata aattggatgt caatctggtt ttcagcacct
    21061 ttcaattgaa ttatcgaaat aagccgcttt tagttgtcga tccgatttta actgagacga
    21121 atatcttggt tatagaagac tttttgaaaa gaaatgaaga actacagcgg ttagagccta
    21181 acagtcatga atcgacggag ttattcactc aaataataga tgtcatgaaa gaaagtggtg
    21241 gccgagtaga tcgaatcatc tatggcaagt tagaaaaatt attcgaaaca aacaatttag
    21301 aaatcaacaa aagggagatc cagcctatgt taaaagatat tttgacggac agcaatatca
    21361 tgatcaaaga aaaagccact acttgggagg aatcgattga actagctgct agaccattgg
    21421 ttaaagaaaa cattgtcgaa gagcgctatg tcgaagcgat gattcgtgca gtgaatgatt
    21481 acggaccgta tatcgtgatt ggcaaacaca tggcactagc ccatgcccga ccagaagatg
    21541 gggtcaataa attaggagtc agcgtagcta cgattgagca accaatcgat ttcggtaatc
    21601 ctgaaatgga tccagtaaag attatttttt gtttggcagc agtagactcc tactcgcatc
    21661 tgaacatcat gaaagaacta attgaattga tcaacaacga agagaagttg gatcgattga
    21721 ttgaatgcac aaatatcgaa gatttcaaac aactgctttt ttcagtagtt gagaaataat
    21781 aaatgatacg ggggagaaca aagatgaaca aattgaacat tttattcgta tgcggtgcag
    21841 gtttaggaag tagttttgca gcacagatgt ctgctgagga tgttttgaac aaattaggag
    21901 tagaagccaa actggaccat tgtgatattt cttccgcagt ttccatgaaa ccagatgtga
    21961 tcatcacagc agaaaatttt cgttcgcagt tcgaaaagtt cgcagttgat cctaaaacgg
    22021 cgatgatcta tttgaaaaat attgtttcaa aaaatgaaat tgaagaaaaa atcaccccag
    22081 ttctgcaaga taagggagtt atctaaatac aaaaagaaaa gagggtaaaa a
//
LOCUS       DAZAJZ010000047        21616 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000047 DAZAJZ010000000
VERSION     DAZAJZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.047"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..1829)
                     /gene="lysS"
                     /locus_tag="ACG86D_001669"
     CDS             complement(333..1829)
                     /gene="lysS"
                     /locus_tag="ACG86D_001669"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN3140807.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1940..2944)
                     /gene="dusB"
                     /locus_tag="ACG86D_001670"
     CDS             complement(1940..2944)
                     /gene="dusB"
                     /locus_tag="ACG86D_001670"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN3140808.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2945..3832)
                     /gene="hslO"
                     /locus_tag="ACG86D_001671"
     CDS             complement(2945..3832)
                     /gene="hslO"
                     /locus_tag="ACG86D_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN3140809.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4049..6160)
                     /gene="ftsH"
                     /locus_tag="ACG86D_001672"
     CDS             complement(4049..6160)
                     /gene="ftsH"
                     /locus_tag="ACG86D_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN3140810.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6250..6795)
                     /gene="hpt"
                     /locus_tag="ACG86D_001673"
     CDS             complement(6250..6795)
                     /gene="hpt"
                     /locus_tag="ACG86D_001673"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN3140811.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6795..8240)
                     /gene="tilS"
                     /locus_tag="ACG86D_001674"
     CDS             complement(6795..8240)
                     /gene="tilS"
                     /locus_tag="ACG86D_001674"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN3140812.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8360..8830)
                     /locus_tag="ACG86D_001675"
     CDS             complement(8360..8830)
                     /locus_tag="ACG86D_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN3140813.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8876..9301)
                     /locus_tag="ACG86D_001676"
     CDS             complement(8876..9301)
                     /locus_tag="ACG86D_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN3140814.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9368..9637)
                     /locus_tag="ACG86D_001677"
     CDS             complement(9368..9637)
                     /locus_tag="ACG86D_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN3140815.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9648..11243)
                     /locus_tag="ACG86D_001678"
     CDS             complement(9648..11243)
                     /locus_tag="ACG86D_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3140816.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11258..14779)
                     /gene="mfd"
                     /locus_tag="ACG86D_001679"
     CDS             complement(11258..14779)
                     /gene="mfd"
                     /locus_tag="ACG86D_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN3140817.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14796..15356)
                     /gene="pth"
                     /locus_tag="ACG86D_001680"
     CDS             complement(14796..15356)
                     /gene="pth"
                     /locus_tag="ACG86D_001680"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN3140818.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15709..16683
                     /locus_tag="ACG86D_001681"
     CDS             15709..16683
                     /locus_tag="ACG86D_001681"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN3140819.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16853..18253
                     /locus_tag="ACG86D_001682"
     CDS             16853..18253
                     /locus_tag="ACG86D_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN3140820.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18331..19053)
                     /locus_tag="ACG86D_001683"
     CDS             complement(18331..19053)
                     /locus_tag="ACG86D_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3140821.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19132..19638)
                     /locus_tag="ACG86D_001684"
     CDS             complement(19132..19638)
                     /locus_tag="ACG86D_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3140822.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19761..21560
                     /locus_tag="ACG86D_001685"
     CDS             19761..21560
                     /locus_tag="ACG86D_001685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN3140823.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6005 a         4719 c         3880 g         7012 t
ORIGIN      
        1 caagtggttc ttttttaacg actcagttat cttatcatgt cgttagtaac atgtcaataa
       61 cttttttgtt atgtttttga ataaatattt tcttctgtct tttggacaag taataatata
      121 ccaactaaat ttagcttggt caacgaattt tttgtatttt ttcaagtttt tttcaataaa
      181 cgattattca taacttttct ttctttatcg ttcgttttcg tcttctttaa tgtctagaat
      241 agtcggcatt agcttttgaa ctttcgttat tatttattcc tttctacaca gcaaaagctg
      301 cctaaggaat tccttagaca gcttttattc gattatctca ttgttgggaa tagcaaaaca
      361 tcccgaattg attgtgcatc agttagcaac attactaatc tgtcgatccc gatgcctaga
      421 ccaccagttg gcggcatacc gtattctaag gcttccaaga agtcttcgtc tacaccgtga
      481 gcttcgtcat ttcctagttc acgttctttt tcttgcgctt cgaatctttc tctttgatca
      541 attggatcgt tcagttctgt aaaggcatta gcaaattcgc gccccactat gaatagttcg
      601 aatcgatctg tgaatcgagg atcttctgga tttttcttcg ctaaaggcga aacttcgact
      661 ggatgtccgt atacaaacgt cggttgtgtc aatgtttctt caacaaatgt ttcaaagaat
      721 tcattgataa catgtccgac acccatagct tcggttactt ctacattgtg tttttcggct
      781 aaagctaatg cttcttccaa tgtcatttct tgccagaagt ctacaccagt ttgttctttg
      841 attgcatcta ccatatggat gcgtttaaag tcactttcta agtcgacagc ttgtccatca
      901 tatgtgattt ttgctgttcc tagtactttt tcagctgcgt tacggataat accttctgtc
      961 agattcatta catcattgaa atcagtatat gctgtgtatg cttctaacat cgtaaattct
     1021 ggattatgtg tagtatcaat tccttcatta cggaaaacgc ggccgatttc ataaactttt
     1081 tccattccgc caacaattag tcgttttaaa tgaagttcta gcgcaatacg caaatataaa
     1141 tcaatgtcca gtgcattatg atgcgtgata aatgggcgtg cggcagctcc acctgcctca
     1201 ttgtgcaata ctggtgtttc tacttcaata tagccattgc catctaaata gcgacggatt
     1261 tcgctgataa tttggcttcg tttcataaat cggtcaaagc tttcgcgatt actgattaaa
     1321 tccaagtagc gttgtctgta gcgttgttca atattcgtta agccatggta tttatctggc
     1381 agtggacgaa gtgcttttga caaaatcgtg atttctgaag ctttgataga aacttcgcct
     1441 gtgtctgttt tcatgatttg tccagttaca ccaaagaaat ctccaaggtc tgcatgtttg
     1501 aatacttcat aggcctcatc acctacttgg tcttttctta cataaatttg aatttggcct
     1561 tcacgatctt gtagatgcgc aaatccagct ttcccttttc ctcggcgagt catcatacga
     1621 ccagctacac tagcggtcaa tcccatttct gctagttctt cttttgttct tggatcaaat
     1681 agttcatgca attcttctga gttgtgcgtg cgttcaaatc gttttccaaa tggatcgatt
     1741 ccatcttcac gcaattgttc cattttttga cgacgtacaa gcatttgatc atttaaatct
     1801 tctacttgat gttgttgttc ctgtgtcaca aaagttcctc cattctcatg ttaatctctt
     1861 tcctataatg ccaatgaaac acaaaaaaag caagccgaac ttggaatttc ggcttgaaaa
     1921 ttcacgaaaa acttagaaat cacaacgttt gtttcaatag tactttttcc gctttttcta
     1981 caaattgatc cagcaggtca ttgatttctt gctgtttttc tgtctgattg attgctactt
     2041 tcactttcgc tgctcgagga atccctttta aataataagc cgcatgtgtt ctgaattctc
     2101 tacaagctac tttttctcct ttgagatctg ctaagcgttg taaatgaagt ttagctgtag
     2161 caattttttc ggctggtgtt ggttcaggaa tcagttcccc tgtttcgaga taatgctgtg
     2221 tgcggtgaat catccatgga ttgcccagcg ctgcacgacc aatcatcaca ccgtctgcac
     2281 cgacatagtc aagcatccat tttgcatctt ctggcgtttt gacatccccg ttccccataa
     2341 atggaatcgt caaatgtttt ttcacttctt tcaatacttc ccagtccgct tttccttcat
     2401 acatctgaac ccgtgtacgt ccatgcatag cgatggcact tgctccagct gattgtgctg
     2461 ctaatgcatt ttctacagca aagacgtgct cttcatccca accaatacgc atcttgattg
     2521 ttactgggat atttactgct gaagaaacag cttggaccat ttcgtatact ttctcaggat
     2581 ctaaaagcca ttttgctcct gcttctgctt tgattacttt atttactgga cagcccatat
     2641 tgatatctat tatatcggca gctgtgtttt cttcgacaaa ttgagcagct tcaactagac
     2701 tatctttatt tccgccaaat atctgtaaac ttaatggatg ttctgtttct tcaatgtgga
     2761 gcatctctaa agtttttttg tttctgaaat gaataccttg gtcactgatc atttcacata
     2821 cgactaagcc cgcaccaaat tctttgacag tgactcgaaa tgctgaattt gttattccag
     2881 ccatcggtgc aacaacgaca cgattaggga tttccacatt gccaattttc catgtgtgtt
     2941 ccacttaaat ccctcctaag gcttctttct tcaatccatc taattcttct tcgctatact
     3001 tgtatttgtt attacagaaa gaacaaaccg cttctgcacc gtgatcttcg tctatcattg
     3061 cttggatttc ttctgcacct aacgcgataa ttgcttctgc aaatttttct tttgaacagt
     3121 cacatttgaa ttgaacaggc attttttcca aaatgttcaa ttcttctgta ccaagcaatc
     3181 ctttcaatat ttcttctggt gtctgtcctt catctaagag ggtagagatt cttggtgtcg
     3241 tttttaattg ttcttcgatc ttgctgattg tttcttcatc ggcacctggc atgatttgga
     3301 tcatgaatcc tcccgccgtc ttgatgctgt cgtcagtatt gaccaataca ctcagtccaa
     3361 cagctgaagg gatttgttct gagatagcaa gatagtaagt aaaatcttca gcgatttctc
     3421 cagaaacaat cggcgtttgc ccagaaaaag gttcttttaa tcctaagtct tttgtgacag
     3481 taaacattcc ttgtgttcca actgctcccc gcacatcgat tttaccttca ctatttagag
     3541 gaagactgag atgcggattt ttgatgtaac ctttcacatc tccctttcca tttgcatcta
     3601 ctacaattcc tgttgctgga ccatcgcctt ggattcgaac agttagtttg tcttctcctt
     3661 tgagcgtcgc accaagtagc agactaccga ccaatgtccg tcccaaagca gctgaggagg
     3721 tgtgccaagt atcatgacgt ctttgtgcct ccgctacggt atttgttgct tgaacggcgt
     3781 atgctcggac gaatccgtcg tgagcaagtg ccttaaccaa ataatctttc atttgttatg
     3841 tactcctttt tccgttttta aactcatagt gaatcatact agcaactact gcttttttca
     3901 actttggaat gcttctttta aaaataattc agaatcatct agaaaacagg acatcaccct
     3961 atttacaact taaataaaga aatctcccga aaaccggttt ttgttccggt tttcgggagt
     4021 catatcagat caattgacct atattttttt atttgtattg atcgtctgaa tgattatctt
     4081 ctgattgttg agaatctgaa tcatgttctg ctttattttc ctcattcagt tcatgttttg
     4141 cttcttcaaa atcacgtttt tcttccgctt gtttttcagc gtctttttct tccaaagcac
     4201 gttttgcttc ttcaaaagtt ttcgcttctt tttcgcttgg ataatcattt gactcagtac
     4261 cttcaggcat cacaccgtgt tcaaacaatg acttgattgc tttggcatcc aatgtttcga
     4321 attccaaaag tttttcagca atcagtttat gctgtgcacg atgagcttcg atgatttctc
     4381 gagctttatc atgagcttcc atcaagattt ttcgtacttc ttgatcaatc tcgaaagcga
     4441 cttgttccga gtaagctttt gtttgaccat agtcgcggcc aacaaacact tgatggtttc
     4501 cttcatattg aacaggacct aaacgatcac tcattccata ttctgttacc atgcttcgtg
     4561 ctagagctgt cgcttgctca aagtcattgg aagcaccagt tgattggaca ttgaagatga
     4621 tttcttcagc tgtacgtcca ccaagtaagc ctacgatttg ttcaaacata tcttctttcg
     4681 tcatcaagaa ctgatcttct tttggtaaag cgatcatata tccgccggcg cgtccgcgag
     4741 ggatgatcgt tactttgtgg acgacccgag cgcgactcaa tactaatccg acaatcgtat
     4801 gaccagcttc gtgataagca accatttcgc gttctttttt gctgattacg cgatcttttt
     4861 tagctggacc tgcaatcacg cgatcttccg cttcatcgat atcagaagca tcgattttct
     4921 ttttatttct tctagcagct actaacgctg cttcattcaa gacattttct aaatctgctc
     4981 cggcaaaacc tggtgtctgt tgagccacaa ctttcaagtc aacgtcatct gccatcggtt
     5041 tattccgtgc atggactcgc aagattgctt cacgaccttt tacatctgga cgtccaacta
     5101 aaatctggcg gtcaaaacgg cctggacgta gtaacgctgg gtctagtaca tcagaacggt
     5161 tggttgcagc aatgacgatt actccttcat ttccgtcgaa gccatccatt tctacaagca
     5221 attggttcaa tgtttgttcg cgttcatcat gtccgccgcc cataccggca ccacgttgac
     5281 gacctaccgc atcaatttca tcaatgaaaa tgatagctgg tgcgtttttc ttcgctgttt
     5341 caaacaaatc acgaacacga cttgctccaa caccgacaaa catttctacg aaatcagaac
     5401 ccgagatcga gtaaaacggt actccagctt ctccggcaac agcttttgct aataaggttt
     5461 tacctgttcc tggaggtcct tctaaaagta cacctgccgg gatacgggca cctaattcaa
     5521 caaatcggcg aggatctttc agaaattcaa caacttcaac tagttcttgt ttttcttctt
     5581 ccgcacccgc tacatcagaa aaacgtacgc gattagcttt tttgtctgct tctttggctt
     5641 ttgatttacc aaagttcatg acacggccgc caccgccacc gccgccgcct tgttgtccca
     5701 tcatcatgta aaagaaaaag atgatgatta cgattggcaa gaagctaagc aatagggaaa
     5761 cccatatacc gctgcttgat tcttctttta ctgttgtagc aactttgttt tcttttgcca
     5821 gttctgttac ttggctcaat gttgtatcat ttggcaaaat gattgttgtg aaatgggtag
     5881 tagtcgtatc tgttgaacct aagatagcta acccaccgct gttagcgacg gtctgctgtt
     5941 ctttgtattc accagtgatc ttatatacac catttgcagg ctgcacttca aattctttga
     6001 tcttgcctgc ttgtagttgt tctgtgaatt ttgagtactc gatatctgac gatcgctggc
     6061 cgttattacc aaaaatgaaa taaacgacca ttaccatcgc taagattacg aggacataat
     6121 acaaggcatt tttcatcatg ccgttgttct ttttgttcat agttgccctc cctaagcgtc
     6181 aatttaattt aacagttttt tatcaatcct gaccattcta ttgtaccata atttaattga
     6241 gatacagcat taatttgatt gataaacact aggtttcaat acaccgatgt acggaaggtt
     6301 acgatatgct tctgcataat ccaagccata accgaccaca aattcatttg gtacgttgaa
     6361 gccaacataa tctgcttcga tatttactac tcttccttca ggtttgtcaa gtagtgtaac
     6421 aattttgact gattttgctt tgcggtattt gaaaagatcc actaaataag caagtgttct
     6481 tccgctgtca atgatatctt caacgatcaa cagatcgcga ccttctacat tcgtatctaa
     6541 gtctttgacg atttttactt cccctgacga gactgttgca ttcccgtagc ttgaaacatc
     6601 catgaaatcc aattccaaat acgtatcgat agaacgaacg atatctgcca tgaatggtac
     6661 cgctcctttt aagacaccta ctactagggg attggtgttt tggtaaattt ctgttaactc
     6721 tttgcctagt tctttgcaac gaacttgtat ctcttcttgt gagatcaaaa ttctctcaat
     6781 atccttttcc aacatcaggt tctccttcca attgcttctt tttgatattt gtaaagaagt
     6841 atgtagtgta ttttatcagt ttcttcacga atactcaaat atgaaaaggc aaaaggtatc
     6901 aagctatata tatttctttt ggcatctgta atgatccaag catgctgcct ttgtgaatta
     6961 ggtactttct gatcgataaa atatctagat actttttttg tgagattctc tgttaattta
     7021 atgcggtctc ctgcttttct tttttcgaca tacagttgca catcagcagg aatccaaatg
     7081 tcatgagaaa atagagacca tgaatctgtt gctttgatgt cccctgcatt ttcaggtttg
     7141 accagaccaa tccattctgt ttcattcaaa tatattcctt gttctggaat cagcaacaag
     7201 cgctggtctt ctgtctggct agttgcttgt ttttcttttt tagagagtac aacaaaatga
     7261 tactctcttt tcagtatcca attattgcca atactgagtt gccattggct ttttttctgt
     7321 tgccactggt ctaaaatcga ctccatttgt tgttctttca aaataattcc tgtttttgag
     7381 aatgctttgt cgaaaaagga gtaaaaaaaa tagtatcgtt cagcatctgt catttcttca
     7441 atcatttctg cagaaaatcg aaattgttcc tcccactgtt cgacttcctt ttcgatcaac
     7501 tgtcccatat atttttgaat gacttggtct gcccattgga tctgttgcga aaattgctgg
     7561 aaatgtctca acgcctgtgt attttcttgt ttcaattgag gaactaccaa atgacgaagc
     7621 cgatttctct ggatatctaa caattggttt gtctgatcct caaagtaaac aagctgcgat
     7681 tccgctgcat aagtatatag gtcttctttg ctaaaactca gcaacggacg aatcagtctg
     7741 cccgtagcaa atggttgtac ttctttgatt ccagaaaaag tatttaactg tcccccacga
     7801 acgattttca ttaacaccgt ctcgatttga tcatctccgt gatgagcagt catcaacgtg
     7861 tcatattgtc cttcttccat cacgcgagtg aattcttgat aacgaaagtt acgagcttct
     7921 gtttctatgg cggatttggc tggatcttcc caaatactta catataaagg gagattcttc
     7981 tcttcacaat atgagcgaag ataggcagct tcttctgctg attcttctct taattggtga
     8041 ttgatgtgga cgatacctaa agaaaaaccg atttggcgtt gcacttgtaa aagtaaatgg
     8101 agcaaaacca tcgaatcaac accacctgat accgctgcta aaattctcga tccttgttgc
     8161 cagaatcctt gttcattccc gtgccgaata aatgactgct gtaatagcgg tgcttttttc
     8221 ccgccttttt caacgttcat cctttccaca ccctttcttc ctaaaaaaaa gagagcggga
     8281 aaacgtctaa aacgacaatt aacctgccct ctgttgatac tatgacccaa atagtcgcac
     8341 tatcattatt tgcggctttt tagctgcgac gtccgccacg gccaccgcgc tttccttctg
     8401 tgtttcgctt caatgaagat aagcggtcat cgctatcttt caaaaatgaa ctcatcaatg
     8461 aatcgaagtc ttctttttga gctgaaggtt gactgcgaga aaaagatttt ttctgtgctg
     8521 gacgatttcg gttgtcacgg ggatcttttt gttgatattt tttaggacgg ctttcttctc
     8581 cagattcagc aggttgttcc tgcgctttcc ggatagatag tccgattttt ccatcatcat
     8641 ttactgaaat aactttaact gttacttcat ctcctacact taaaacatca tggatgtctt
     8701 ttacatagcc atttgacact tcgctaatat gaactagacc cgtttttcca gcacctagat
     8761 caataaacgc accaaaattt gtgattcctg atacttttcc ttgcagtttt gctcctacct
     8821 cgattgacat aaaaaaaatg ttcctcctaa ttatttccct tttttatatt cctgcttatt
     8881 ttgcagttgt agaactagtt gattgatctg tttcatttgt cgagctgtct gtatttgatt
     8941 ctaatatagg ataaactttt tcaccgtctt ttgagtaaaa gaatttgctt ctggcaagtt
     9001 tagctacata ctcatcatct ttcagcaatg aaacatcttg gtgcagctga tcaacgtctg
     9061 cgttgacttc tttcatttca gtatttgctt cgactctcaa ttcgttcaat tgatgcatcc
     9121 tttgcatgtc atggaaaatc tgcacaccta ttatcgcgaa aatgaccaaa gcaccaacaa
     9181 aaattgcagc cagtctgcgt cttcgaaaaa tcagttgacg ttgttgttgc tgaaattcag
     9241 catacttttt ctttgcatat tccgtatcta gcgcagcgat tttattgctt tttttcgtca
     9301 cttggattcg ctccaatctt tttgattgtc tttttcatta taccaatatc gtgaaagctt
     9361 gtctaatcta tttctctagt gtttctgtgc gattttcaga aataatttcg tacatttttt
     9421 gtgcatcttc ttttttggtt gattcatgta attccgtaat tttcacttcc aacgttttgt
     9481 tgccaaactg gactttcaaa atatcgccta ctttgacatc actggaagat ttcgccaatt
     9541 taccgttgat ttgaattctt cctttatcag ctacttcttt tgctactgat cgtcttttga
     9601 tgattcggga aatttttaaa aatttatcta atcgcatatc ttattcctca ctttttaatt
     9661 tttttattta atcgcaggat tttcttacca aaaggcaaca ttagccattc tcgtactgta
     9721 aacagacgaa gacgaatcac tgcataaatg aacacagaag ccccgatccc aactcctatc
     9781 acgctgacaa aaaaagcagt ggaacgatga gttacaggac caaatactat attcaataca
     9841 ccataataca gcagcagtac tactgtcata caaagaaggc tgaacatcaa cttccgtcca
     9901 aaatgccgtt ctttccaaaa agcatttagc tcttttgttt caattcgaac aaaataccaa
     9961 agcgtcacac ctaatcctaa aagggtagac aggcttgcac ctgctgttcc taataaaccg
    10021 gtcagaaaag aagtagtcaa tccttttacc aatagtcccc atcctgcgcc ttttaaagaa
    10081 gggcgaaacg agttcttgct ttgagcaata ctttgataag cttggatgac tgcagtgaaa
    10141 gcaatggaaa aaacaaataa tacaagcgct gcatttcccg cataatcttt aaacaacgca
    10201 taattgatat aagggagcag caaggctaat cccaaagacg cagctaatgc taaagcggtc
    10261 gtcaatctaa gatagatttt ggctgtctgt aaaaactgac gatagatccg gttggtcaaa
    10321 tgacgagtca acgcaggcag gaatgtcgca cttaatgcag tggcaatcac taaacctaat
    10381 tgtactaaag gctgacctcg atcatagacc ccttttgcta tcttcgcacc gtattctgac
    10441 agtccgtaaa cttccaaagc attttttaca aagaaagagt cgatcagctg gaaaaagata
    10501 agcaatccac tataaatcga aaccagtccg ccttctacca gaaaacgccg gatcaagggc
    10561 tttgacggct gtttgaacaa tggaaaccgt cggaagctta acgctcctcc atgaatcttt
    10621 tgttcatagt agtgaaggat cccatatgcg cataaaccgc caaaaacagc tccactcatt
    10681 gctgccgtcc ctgtttggta caccgtccat ccaagctttc taaaagcaaa cgcactacag
    10741 acaatcacaa aaacacgaac gaactgctca actacttgag aaacagcagt tggttccatc
    10801 aagaatcttc cttggaaatt ccctcgataa aaagatagac caggcatcag caaaaaggta
    10861 aaggaaacta cttggatcag cggtttcaat tgtatgtctc ccatcatcca agcaatccag
    10921 ctgctaaatg caaagacaaa caaccacaaa aagacacctg tccaaaatac taagggataa
    10981 agattttgca aagcttgctt tcgctcgaaa ggattctttc tttctgcaac gtactttgat
    11041 atgaactgcg gaagaccgga taaagctaaa gtcattgcta tcccataaat tggataaact
    11101 tgctgataaa cataaaaacc ttcgtcgccg actaagtttt ggaggggaat tcggtaaagg
    11161 gcacttaata cttttgctat gaaagaagca atcgtcaata caaatgctcc ttgcatgacg
    11221 cgccgcattt ctttatgatc caaatcattg ctccttccta cgctgacaga tatttttgct
    11281 cacgcaacgc tttgacaaac ttttggattt cctgaagcca tactgcttct tccatttggt
    11341 tggggatcgt caagcgtatc accatctgtt ctttttcaac gcctagactg gcttttagtt
    11401 ttgtcgctgt cagtgcttcg aataactgtt ccacactata gcgttttgtt ccaactttac
    11461 ttagagtaaa gactatatgc gcctcctgtt tccttatcgt ttctaggagc gctcgatcgc
    11521 catccatttt gatctgccca attgtcaaga gatgtgctac ttcttctgga tattctccaa
    11581 aacggtcgag cagatcatct tctagttcat ctagcatttc ttgcgaatca agttcacgaa
    11641 ttcgtttata gatctcgatt ttctgccgtt gatctgccac gtatgtttca ggtagatacg
    11701 catcgacacc gaggtcgatc tcaacagtgg tcttctccac ttgactattc tttccttgtt
    11761 tacgagaaac agcttcgctt agcatttgag aatacatatc gaaaccgact gcatcaataa
    11821 atccatgctg ctgagcacct aagagattac ctgcaccgcg aatggaaaga tcccgcatcg
    11881 caatcttgaa tccagatccc agttctgtaa aatctttgat tgcctgaaga cgtttttcac
    11941 tgacttcgtt aaggatcttc tgctgctcat acataaagta agcatatgcg acacggctgc
    12001 ttcttcctac ccgccctcgc aattggtata acgtggataa tcccatataa tcagcgttct
    12061 caacgaacaa cgtatttgcg ttcgggatat ctactcctgt ttcaatgatg gttgtagtga
    12121 caagaacatc atattgacct tcaataaatt caaaaagcgt attttctaat tgcgcctctg
    12181 tcatttgccc atgagcataa ccgatccgtg cttccggaac gagttcttgg atttcttcta
    12241 ctttttgttc gatcgtttct acccgattat ataaatagaa tacttgtcca cctcgtccca
    12301 tttctcgatg gattgcttcg cgaatagccc caggattttt ttccataaca tacgtttgga
    12361 tcggataacg attcgcaggc ggtgtttcga ttactgaaag atcccgcacg cctaacatcg
    12421 acatatgcaa agttctcgga atcggtgttg cagtcagagt caaaacatcc acttgtgcac
    12481 gtaactgttt caatcgttcc ttatgtttta caccaaaacg ttgttcttcg tcaatgatca
    12541 gtagacccaa atcgctaaat tcaatatctt ttgacaaaat tcggtgggtt cctacaacaa
    12601 tatcaacttg acctgtacgc agttgttcga tggtttcttt ttgttgcttt cttgttctga
    12661 aacgacttaa tagcccgata tttacgggga aaccttcaaa tctttccagc atcgtttcat
    12721 agtgctgctg tgccaagatc gttgttggaa ctaaaaaggc tacttgtttg ctttccttta
    12781 tcgctttaaa ggccgctcga agcgctactt ctgttttccc ataaccaaca tcacctacga
    12841 gtagtcgatc cattggtttt tctttttcca tatcccgttt gatctccgct gcacttctca
    12901 actggtcatc agtttcagag taggggaagg cattttcgaa ttctttttgg taggcgtcat
    12961 caggtccaaa agcatatcct ttttctgact ctcttgcggc atataatttg attagatcgt
    13021 cagcgatatc ctcgattttt gaagagactt ttcgcttggt tttcgtccat tcacttccgc
    13081 ctagcttatt gatgcgaggc gctttggctt cagaggcgac atatttttgg atcagattca
    13141 gttgagtaac aggaataaac agtttgtcgt cgttttgata caaaatagtc atatagtctt
    13201 ggtgaacgcc atctacttct agcgtttcca tcccaatgta tttcccgata ccatgattgg
    13261 catggacaac gtaatctcct gcttttaact cattatagct tttcaatcgc tctgcgtttg
    13321 ttactgtttg acgtctcgtt tgttttttcg tcgttttttg gaaaatttct ttttctgtga
    13381 tagctactat tttttcttga ggaagttcaa aaccagcctg caaagctcct tcaactaatt
    13441 gtacctgtcc tctgatcaat gcattttttt ctgtttctac tactaatatg tcttcatctc
    13501 gaagcatttg ttctgctttg cgtattctgt cctttgtcgg aataaagatc acaacagtct
    13561 gtttttgctt gcgccaacgg tccatttctg ttttcagcaa tggcatttgt ccgaagaact
    13621 gttgcatcgt ccgatattga aaattatgga ttgcttggaa acgaagattt cccatccctt
    13681 tttggaagag tgagaaaaag gttgtggtga acttttcctt ctgcacgatt ttttgtatat
    13741 ctgctgaaaa tgtctgctca gaaaacaccc gcaattcgct gattttttga gtctgccatt
    13801 ctgccgcttc cctttcgatt tcccgatttg tttccatgat tcgctgataa tcatcaacga
    13861 ctagtaaact atttgaaggg aaataatcca agatcgtcac agattcttgg taaagaagat
    13921 ctgagtagaa ttttgcttct tctgtcggga cacctgcttg ccagcttgaa gtcagctgac
    13981 cgaagtaatc ttgcaaaaac tcgcggtctg cttcttctgt ggtcgcagca agccgttttt
    14041 ctagcatttt ttgcatttgc tctattccat gttctagatc ctctggtccg tagacttgtt
    14101 cactcgttgg ggaaacccag atttcttccg ttttttcgat tgagcgttgt gtttctgctt
    14161 caaaataccg catagaatct atctctacat caaatagttc tacacgaaca ggataggttg
    14221 tcgttaacgg gtaaatatcg ataatgcttc ctcgaatact gaattctccc ggttttgcga
    14281 ccatctcttc tcgtacatag cccatcaaag tcaattggcg aggaagattt tccacgtcta
    14341 tctcatcacc aatatgccaa ttcaattgcg cctgtagcca agttttctta ttcggcagtc
    14401 gtttgtgcaa agatgcgact ggcaacaaat agatacctgc ttttttttct tgaagtgcgt
    14461 taagcgttgc aactctctcg cctcgcgctt ctggagaaga aaaggccatt tcagcagaaa
    14521 gcacttcatc cactggaaat aaatggatct gatcttgcgg aatcacatgt tgcagatctt
    14581 ctgctaactg attcgcataa tacaaatttg gcatcagaat aatcatgttt ttatttgttt
    14641 tcttaaacgc tcgagcaaat agaagtgttt tcgctgagcc cgctaatcct gtgattagtt
    14701 gtctaggcat tttttcttct aaattgctga cccattcttt tacttgcgga tcttttccga
    14761 ctaattgaat aatatccatt ccttttcctc cttcctcaat tgaatcgatt catcgcatcc
    14821 acgaacgaat gcccttctat gagatattcg actgctttaa cagattgaca catgctttct
    14881 tctatcaaag gctgattttc tttgctgaaa ggactaagca catgctggac tactgttttt
    14941 ttcccttctg gcctgccgat tcccaccttg attcgatcaa agacattcgt attcaaatgg
    15001 gaaataatac ttttgatacc gttatgcccg ccggcacttc ccttttgacg gagccggatt
    15061 tttcccactg ccagatccaa atcatcgtag attacgacta attcctctgg ataaatccca
    15121 aaataagtca ttagtgggcc gactgcacga ccagattcat tcatgaatgt ttgtggtttg
    15181 actaaaagga tcttttctcc attatgaaaa aattcggcaa cttctgcttc gaacggattt
    15241 tttttgaaag tagcttgatg ctcgcgtgca attttatcca cgaccataaa gccgatattg
    15301 tgttttgtat attggtattt ggtacctggg tttcctaacc cgacgatcat tttcattttt
    15361 gttttccacc taattctaaa aagcagcaca aaaggctgga ctgctaatta gcggtccagt
    15421 ccatcgagct tttttctttt ttacacttgt caatgcatag tataacacaa aacaaggcgt
    15481 ttcggctaaa agagaatcgt taataaatga taaagaaatt aaaaatttct tcaaagttta
    15541 atgaaataat tgatatataa aaatagtgaa ataacgcaca tgtaagcttt tacatataat
    15601 acaaccttgt tatctgttat attttatagc caaatagcgt gacatggggc ttgagaaatg
    15661 ttagcataag catgggaaaa aacaaatcaa tggaaggatt gatactaaat gactgcagct
    15721 gcagaaaaaa aagatcatca aaaagttatt cttgttggag acggcgccgt aggttctagc
    15781 tatgcctttg ctctagttac tcaaaatatc gctcaagaag taggtattgt ggatatcaat
    15841 actgctaaaa ctgaaggaga cgcaattgac ttgtctcacg ctttggcatt tacttcacct
    15901 aaaaaaatct attctgctac ttacgcagac gcacatgatg ctgacttagt tgttattaca
    15961 gctggcgcac cacaaaaacc aggtgaaact cgtttagact tagtaaacaa aaacctaaaa
    16021 atcaaccgcg atgttgtcac acaaatcgtt gaatcaggat ttaacggtat ctttttagtt
    16081 gccgctaacc cagtagatat tttgacttac tcaacttgga aattctcagg tttccctaaa
    16141 gaacgcgtaa tcggttcagg tacctctctt gactcagctc gtttccgtca agcacttgcg
    16201 gaattagttg acgttgatgc acgtaacgtc catgcttaca tcttaggtga acacggagat
    16261 tcagaattcc cagtttggtc acatgcaaat gttgccggct tgcaaatcta tgaatgggta
    16321 aaaaacaacc cagaaatcga tgaagaagct atggttaacc tattctttgg cgtacgtgac
    16381 gctgcttaca caattatcga gaaaaaaggc gctactttct atggtatcgc agtagcatta
    16441 gcacgtatca ctcgtgccat cttagatgat gaaaatgctg ttcttccttt atcagtattc
    16501 atgaatggcg aatatggatt gaacgacatc tacattggtg cacctgctgt tatcaaccgt
    16561 caaggtatcc aaaaagtaat cgaaattcct ttaagtgaca gcgagcaaga ccgtatggct
    16621 gcttctgcta agcaattaaa agaaattcta gatgaagctt ttgctaaact agacgctgaa
    16681 taatctttga acatgacgga aaacatctat tcggtgtttt ccgttttttt attttatcac
    16741 ccttatggtt tctaaatttt ttcataaagt agagataacc ttcgtctttt cctctaaatt
    16801 atgctatcat gagaccgttc caccctaaac ttttacttaa ggagttgtcg atatgacaag
    16861 atcttcgcat cgaaaaaaat cgaaacatgt tgctttgctt tcagttcttg gtgttctctt
    16921 agtgctgatc agcttttata cctatcgcag tacatactac acaaagcatt ttttgccgaa
    16981 tacagaaata aatggaataa atgtcagcaa tctaacagtc gaaaaagcaa atgaaaaatt
    17041 aaaagaagct tattctgata aaaaactggc tattaaagaa gatggaaaag tctggcagga
    17101 agtaccaaaa tccgagctcg gctacaaaga tgatttcact tcggagcttt ctccgatcct
    17161 aaatgaacaa aacggatgga cttggggaat gacttatgtc tcagcggcag aaaaacaaga
    17221 aatagatcct gtcagtcaaa accaccaaaa actagacact accgttcaga cattgacaac
    17281 taagctgact gatttgaaca aagaccggac acctactact gatgcaacga tcgaaaaagc
    17341 aggtgactct ttcgttatca agcctgaagt caatggaaat acgatcgacg tcgatgctgc
    17401 ggtcaaacaa ttaaaaagtg cggtaaactc tggtaaagat acaattgaat tgactgaatt
    17461 caaagaaaaa ccaaaagtaa catctgaaga cagctcactg aaagaacaac tcgcatccat
    17521 gaatgctatc gcaaatgtaa aagctactta cagtataaac ggtgagactt tccagatccc
    17581 atcaagtgat attatgagtt ggctgaccta caatgatgga aaagtcgatt tagatacaga
    17641 acaagtacgt caatatgtaa cagatttagg cacaaagtat aacacaagta cgaatgacac
    17701 aaaatttaaa agtacaaaaa gaggagaagt tacggtccca gttggcactt acagttggac
    17761 gatccaaaca gacagtgaga cagaagcatt gaaaaaagcg attctggcag gccaagattt
    17821 tactcgttca cccattgtgc aaggtggcac aaccgctgat catccattga tcgaagatac
    17881 gtatatcgaa gttgatctgg agaaccagca tatgtggtat tacaaagacg gaaaagtggc
    17941 ccttgaaaca gatatcgttt ccggaaagcc aactacacct acacctgctg gagtcttcta
    18001 tgtttggaat aaagaagaag atgcaacttt aaaaggaaca aatgacgatg gaacacctta
    18061 cgagagtccg gtaaactact ggatgccgat tgactggaca ggtgttggta tccacgattc
    18121 agactggcaa cctgaatacg gtggagactt atggaaaacc cgcggttcac acggctgcat
    18181 caacacccca ccaagtgtaa tgaaggaatt attcgggatg gtcgaaaaag gaacgcctgt
    18241 attggtcttc taacaaaaat aatatataaa aagaaagcac ccgtacgatt ggtacaggtg
    18301 ctttcttttt attatctaac aatgtgctac ttacatttca gcagcagtaa agacttctga
    18361 aacatcatca tcgtcttcta gcttatctac tagacgttcc aattgctctt tttgttcctc
    18421 atttaaatca atcgtcgttt gtggaatcat cgtcaattct gcttgcgcca aagtgaactc
    18481 tttttccaac tcatctcgta cagcagcaaa atcttctggt gcagtataaa tctcgaatac
    18541 ttcaggagaa gtttgcagat cttctgcccc cgcctctaat acatcctcaa acatcgtctc
    18601 ttcatcaaca gtcaacccat cgcgttcaat agcgatatat cctttgcggt caaacaagta
    18661 actgactgaa cccgtttctc ccagtgatcc accgtttcga gtaaaagcta ctcggacatt
    18721 tgtcgctgtc cgattacggt tatctgtcaa tgcatggact aagattccta ctccacccgg
    18781 accatatcct tcatacgtga tttcatcgta attcgtttca tctacggcac tcgtagcctt
    18841 tttaatcgcc cgttccacat tgtcgttagg catatttgca gcttttgctt tgtccacaac
    18901 aagccgcaaa gctggattca tagatggatc aggaccgcct gctttagctg ccatataaat
    18961 ttcacgagaa agtttctgaa agactttccc tcgttttgca tcttgggcat tttttcttcc
    19021 ttggatatta tgccatttac tatgtcctga catgattttt cctcccttgt tcttttgaga
    19081 atacacttct attctagtac tatttcacaa aaaaacaaag ccacagccaa tctataaacg
    19141 cttcttgaat gccgcaaaaa gcacacataa tatcacagca gtcagagcac cgatcgaatc
    19201 caacatgaca tcttggaaca taggtgttcg tccaccggtc atcatctgat ggaattcgtc
    19261 caaacctgcg taccctgttg ctgaaagcca agcaaggatc gctgtcagcc accaaatttt
    19321 tagcttgggg aaaacaccta aaaacaagct gcctcccaaa ataaaatagg taccgaaatg
    19381 tgcgcccttc cttaaaaaga actctaaaaa tttggcgtag cccagatggt tgatactcac
    19441 catacttcca ccataattaa aacgtatacc cgctaatgtg tctttaaatg gctgattagg
    19501 cagccagctt tctaatcgcg aaacttgtga ttgctgtccg taagtttgag aagaactgta
    19561 aaataagatt ataacgacta tcagtgcgat tactaaataa atatttccgt ttttcatttg
    19621 tttttttgaa gaattcataa gtcacctctg tattttatct tagacttttt ttgaagaaac
    19681 aacaatcatt ttcatagaga cttcttccat aaaaaagtgc tatacttaat tttgtattac
    19741 aatatgaagg gagttttttc atgagttatt cattaaattg gaatctagat tctatttttt
    19801 ccggaggcag tcattctgat gctttgaacc aacggatgaa gcaattggaa gaccaaatga
    19861 acgagtatta tcaccgcgta acaaaatggt cacctagttc tgacaatgca gagcagttaa
    19921 acgctattct tcaattgcaa gaaacgatta cgaatggttt tacccaatgc agcagctata
    19981 ttactgcatt gctttctgcc aatgtcaatg attcagatgc aaaggtctta tcaggcaagc
    20041 tttacgcaat gctgcctcgt ctgcaatcag ccgaaactgt attgtcaaag aaatttgcag
    20101 aaatatctga caatgattgg aatcagatgc tttctcaatc ttcgtttgaa acaattgctt
    20161 ttcgtttaaa cgagattcgt cgtgatggct cacaattatt atcagaagct gaagaaaaca
    20221 ttataaatac gctttcttta gatggtctaa atgcttggag cagtcattat gatacgattg
    20281 tagccagcat ttcgattccc ttcgaacaag atggacaagt agtcgaatta tctgctggac
    20341 aagccttcaa taagatgatg ggcgatccag atccaaaagt cagagaaaca cttttcgcag
    20401 catgggaaga agcctggaaa gaaaaggcac ccctcttcac ggatactttg aatcatttag
    20461 atggttttag actttctgac tatgaacttc atggtgtcac agattttcta caaaaaccat
    20521 tagagtacaa tcggttgaag aaagaaacat tgtctgtcat gtgggataca atccagaaaa
    20581 acaaacaacc ccttgttaat tacttgacta gaaaagctaa tcttttcggt aaagaaaaaa
    20641 tggaatggca agatcaagat gcacctatta ttttaggcga tttgaaagaa aaaaccttta
    20701 gttttgacga agcagcagca tttattatcg aaaactttaa caaattcagt ccaaaaatgg
    20761 ctaaatttgc ccaatctgct tttgaaaaga gctggatcga agcagaagat cgtcctggga
    20821 aacgtcctgg tggttattgt actgagttac cagaaacaaa agaatcaagg atctttatga
    20881 cctacagtaa ttctgttaat gaggttgcga cacttgctca tgaactagga catgctttcc
    20941 atagcagcac gatgtgggat ttaccttctt taaatcgcga atatgccatg aatgtcgcag
    21001 aaacagcaag tacattcgca gaattgatcg tagccgatgc tacattaaaa gtcgcaaaaa
    21061 caaaagaaga aaaaatcaat ttgctagata caaaactgca aaatgcttta gctatgttca
    21121 tgaatatcca ttcccgattc atttttgaaa atcgattcta tgaagcacgg caagaaggat
    21181 tagtctcaga agataagatc acagagatga tggttgaggc gcaaaaagaa ggctatcaag
    21241 gagctttagc tacttaccat ccatatttct gggctgcaaa attgcatttc tttattgatg
    21301 acgtaccgtt ttataacttc ccatacacat ttggctacct ctttagtttg ggcatctatg
    21361 cttacgccaa ccaaaaggga gcaaattttg aagaagaata tatccattta ctacgcgata
    21421 ctgcctcaat gacgacggaa gatcttgcga agaaacattt gaatgtcgat cttacccaac
    21481 cagatttttg gcaagcaggt atcgacatgg tgactgaaga tatccaaacg ttcatggaat
    21541 tgacagaaga atttatctga tttctatttt atcacaataa aaaaagagtt agtccagaat
    21601 gggctaactc tttttg
//
LOCUS       DAZAJZ010000048        20755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000048 DAZAJZ010000000
VERSION     DAZAJZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.048"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..1289
                     /locus_tag="ACG86D_001686"
     CDS             87..1289
                     /locus_tag="ACG86D_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN3140824.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1466..2734)
                     /locus_tag="ACG86D_001687"
     CDS             complement(1466..2734)
                     /locus_tag="ACG86D_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN3140825.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(2785..2852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2895..3542)
                     /locus_tag="ACG86D_001688"
     CDS             complement(2895..3542)
                     /locus_tag="ACG86D_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN3140826.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3807..4310)
                     /locus_tag="ACG86D_001689"
     CDS             complement(3807..4310)
                     /locus_tag="ACG86D_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3140827.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4434..5198)
                     /locus_tag="ACG86D_001690"
     CDS             complement(4434..5198)
                     /locus_tag="ACG86D_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN3140828.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5306..5581
                     /locus_tag="ACG86D_001691"
     CDS             5306..5581
                     /locus_tag="ACG86D_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN3140829.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(5929..7080)
                     /locus_tag="ACG86D_001692"
     CDS             complement(5929..7080)
                     /locus_tag="ACG86D_001692"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3140830.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7100..7822)
                     /locus_tag="ACG86D_001693"
     CDS             complement(7100..7822)
                     /locus_tag="ACG86D_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3140831.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7815..9266)
                     /locus_tag="ACG86D_001694"
     CDS             complement(7815..9266)
                     /locus_tag="ACG86D_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN3140832.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9408..10490
                     /locus_tag="ACG86D_001695"
     CDS             9408..10490
                     /locus_tag="ACG86D_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3140833.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10824..11762
                     /gene="yidC"
                     /locus_tag="ACG86D_001696"
     CDS             10824..11762
                     /gene="yidC"
                     /locus_tag="ACG86D_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN3140834.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11943..12347)
                     /locus_tag="ACG86D_001697"
     CDS             complement(11943..12347)
                     /locus_tag="ACG86D_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3140835.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12363..13172)
                     /locus_tag="ACG86D_001698"
     CDS             complement(12363..13172)
                     /locus_tag="ACG86D_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3140836.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13159..14049)
                     /locus_tag="ACG86D_001699"
     CDS             complement(13159..14049)
                     /locus_tag="ACG86D_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3140837.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(14062..14541)
                     /locus_tag="ACG86D_001700"
     CDS             complement(14062..14541)
                     /locus_tag="ACG86D_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN3140838.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14558..16324)
                     /locus_tag="ACG86D_001701"
     CDS             complement(14558..16324)
                     /locus_tag="ACG86D_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFN3140839.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16370..17311)
                     /locus_tag="ACG86D_001702"
     CDS             complement(16370..17311)
                     /locus_tag="ACG86D_001702"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN3140840.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17308..18303)
                     /gene="lacD"
                     /locus_tag="ACG86D_001703"
     CDS             complement(17308..18303)
                     /gene="lacD"
                     /locus_tag="ACG86D_001703"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN3140841.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18296..19489)
                     /locus_tag="ACG86D_001704"
     CDS             complement(18296..19489)
                     /locus_tag="ACG86D_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3140842.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19507..20235)
                     /locus_tag="ACG86D_001705"
     CDS             complement(19507..20235)
                     /locus_tag="ACG86D_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3140843.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         6025 a         4465 c         3626 g         6639 t
ORIGIN      
        1 ctaacttttc tatattcgat atatttacta ctaaatgatg ctatcttttt taagaaaaaa
       61 tttacaaatc gaaaggagtt cattcaatga atcttactag agacctgata tttttagtta
      121 tctatatttt aggaatgtca ggcatcctct attggcgcaa gcgccaaagc gatcccgctc
      181 tattctttta ttgtgggacg tggactttta tgatgatggt cacttacttc gtcgttttag
      241 aagtttttta tcatacattt tattttttga ttccattgct atttttcagt atttttgcat
      301 ttttctattt taaggaaaaa cgtaggttaa tcaatggcgt actttttaat acgtttttga
      361 ttgtctttgg tggttatcta gggtggctac taatagagac gcaaaatatc tttatcggtg
      421 ggttattggc tttgatcggg ataccaatat taattctttt ggcaatcggt atctacattt
      481 tgatcatttt tttattttgg aatggtgttg tagtcatgaa aaaagaatcc cgttctttgg
      541 ccaacctttt gactctcttg ttaggagtcg ctttgactat ctttttgata ttggactata
      601 cattagtcca ttggctccca caatgggcaa atgctctatt tgctgtcatt ccgtttattt
      661 ttgcttatct actcatcgtt ttctttaatt ttatgactgt ttcaattttg tatcagttca
      721 accgtccaag gtataaccaa gattatatcg ttgttctcgg cgctggcttg attgatggtg
      781 aacgcgtatc tcccctactt gctaaaagga tcaataaggc gatcgcattt tattgggcac
      841 aaagtaaagc aactttgaat ccgccagtct tgctcatgtc aggaggaaaa ggaaccgatg
      901 aaaaagtagc tgaagccatt gcaatgaaac aatatgcttt agaaaaaggg attcccgagc
      961 gtgatatttt agtagaaacg aattccacaa cgacgctaga aaatatgctg ttttcaaaag
     1021 aaataatgga tcagcaaatg aatgggcctt atcgcgcgat ttttacttca aataattacc
     1081 atatctttag agctggacta tatgcgcgac aagcgaaact caatgcaaac ggaatcggtg
     1141 caaaaactgc actatactac ttaccgaatg ctatcttaag agaatttatt gccatccttg
     1201 tcttacacaa aaaacgccat cttatcatct gtggcttaat gattggcttt gcagtatttt
     1261 ctgctcttat tctcaaattt gctcaataaa aaagagcgtg ggacaaaact aaaaatcagt
     1321 tttgccacag actctaactc cgaataaacg gtgggagcag aagcaactcc ttcggaaata
     1381 agcgaaattc acaaaaattt aaaaagcaat tttcgtgaat ttcttcttat ttctcggagt
     1441 taaacacttc tgtcccaacc tctttttatt tctctttttg tcgtgagatt cttccaagga
     1501 tcggtaggaa caagattcct tttttcactt caactttttg cgcagaatag attccactat
     1561 ttcctgaaca cataaagcta atcaaacaga tcataaagaa gtagctggct gcttgacctc
     1621 caaaaagttc gatccccata acaaagcaag caatcggtgt attcgttgct ccagagaaca
     1681 ccccaataaa ccctaatcct gctaaaaaag gtactgatac atgaagaatg ggtgctaatg
     1741 tcgcacctaa agttgcccca atctcaaaca aaggggtcac ttcgcctcct tggtatcccg
     1801 cgccaagtga tagaacggta aagccaagtt tcccaagaaa atcataagga tgagcacttc
     1861 cattaaatgc atcttgtaat aaaggtaagc ttaatccgag atatctttgc gtctgtaaaa
     1921 tcactactac gagtaccacg actgctcctc caataaagtt gcgtaaaact ggatggggga
     1981 tccgctttgc ataccaagct tttaaccaga cgatcgaacg gctaaagagc cagccaatga
     2041 taccaaatgc aatgcccgca agcaacaatt tgaagaaaag tgaagggtcc catgcaggaa
     2101 tcgcccccat ttgataatgg gtatgcttga cattgaaact ttccgttaca aagttcgcaa
     2161 aaagtgctgc aaaaaagctt ggaaacagtg cttcggttct cacttttccg attgccaata
     2221 cttctaatcc aaacaatgtc ccagccaagg gtgtcccaaa tactgagcta aaacctgcgc
     2281 tgatcccact gatgacaaga atctcacgct cgattttgtt caattttagc caacgagcaa
     2341 aattctcagc aagtgccccg cccatttgga ccgctgtacc ctcccgacca acagaaccac
     2401 caaaaagatg cgtggtaatg gtgccaaata aggtcagtgg gatcagccgt aatggaatct
     2461 tttcatttcc tccattccct tgatcgatca ctaaattgtt tccccgggaa gaattgcccc
     2521 catacgtcgt atatagataa gcaaaaagtc ccccacttat cggcaaaaga aaaagtaacc
     2581 aaggatgctc cagccgaata gctgtcacat cagtcaaact agttagaaag aatgcggata
     2641 aacttcccat aaaaatacct aaaattgctg tgatcaataa ccatttgatc atatagatga
     2701 tcaccttcaa tggatcaggt aatcgatttt ccatctcatt tcctcctcaa aaaaacgcct
     2761 actaaaaaaa ggggtagccc cgtttcttta gtaggcgtca ttagttatct tacgatcaac
     2821 gtttaaaggc gaacaccatc acctattcac ttcaccaaat aagatagcat atttttcaga
     2881 aaaatacaat gaaatcaccg ctcacgtttc actcgttctg tgaattcgta aaaaggagct
     2941 aattgtacca actgctgatt acattggtca ctatcatgac aaatataatt tccttttttc
     3001 gtgtatgttc catctgaacc agtttttgtg gtagaaagaa agagtgaaac atttgaagtt
     3061 ctttgacaaa tcgaacaaat tcctttgttg attgtaggag ataatgttcc actgatccct
     3121 tttagttttt cttcttcata atagatcata aatttttttt ggctacctgg atcattccaa
     3181 ccaatataag tatgttccct taaatccagt tgattccaat ctggttgttt taactttttc
     3241 acttttcgga ataatttatc gatctgcggt tttgtcggtt gtatgaatgg aagaacgatc
     3301 actttcaatt cctctaatag tcgttctgct ttttcttttt ttagagtgac gtctagtacc
     3361 ttttccataa attcatttac agctgtatta ggatgaccaa gtacctcgtg gatcttttcc
     3421 gcagctaatg cttggactgt ttggactgtc tttggatcat tcactgaact ataggcgctt
     3481 aacagctgct cgacttctcg cttgataaaa taatattgat atggtttgat tgttttttcc
     3541 atgactttga gtcctttctt ctttcaaaat cggctacttt gaagaaaaaa gtgatctttc
     3601 tatcaaagtg cctttagata aagccctcaa actcttttaa ataagataca catcgcatct
     3661 tatcatcacc ccctttattt ttatgttggt tatgtacttt ttcatctcaa caaagtaaac
     3721 actaaaaaga ccaattagtc aatgtctaaa caaaaaaccc gaggattttt ttcaaaatac
     3781 tcgggtcaaa tacttagtta actaccttaa atcgattgtt ctttaccgaa atatcgttgc
     3841 catttttgtt tcattgaagc tgtcattggt tgagctgctt ctttgactgc acaatattta
     3901 tccacaaaag taacgatata accttcttta tatttaggag gggcaatcgt tgctccccac
     3961 atatgtttca aaataatatc acgctctaga tctgaaagct ccgtgatttt ttcagcattt
     4021 ttcacagcga tccttgggtg aacatacgca tgtgtacctt catcgaactt cgtcgtacgc
     4081 caatcgtagt aaaataaatc atgtaaaagg cctgctcgag cagtcgctct tgcatcaccg
     4141 ccccattttt tcgctaattt gtagctatag taagacacac tgattgaatg ctctaatcgt
     4201 gtcgagtgga catgttgcgt gtaatttgct aatttcttta ctgcatcagt ttctaataga
     4261 tcatttatat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
     4321 ctgccacctt tttgaattcc atttatcttt gaaattgaac ttagtatacc actagtgttc
     4381 gtaatattct agtgaggttt tcgcaaaatg cagaaagctt aatagttcct taactatacg
     4441 gagaagtgat aaagcatgat gcccgctgca actcctacat ttaaagactc cgcatttcct
     4501 tgaatcggta tatacacatt tttatccgtt agcgccagca cttctggacg tacgccttga
     4561 ccttcattcc ccatcacgat cgcataattt tctgttgtct ggatatcatt cagggacact
     4621 gcttcttcat tcagttctgt tccataggtt ataaatcctt gctctttaag gataggcaat
     4681 agttttgtta gatcctcttg gatgatcggt aaatgatagt tactcccctg catgcttctt
     4741 aatactttgg tactgtagac atccgccgtt cccttaccta gtacaacaca agatagacca
     4801 aaagcatcgg ctgtgcggat cattgtccct acattgccgg gatcttgtac gttgtcgagc
     4861 aacaaccaac caccttcaaa attcggtatc tctctttttt cagcttccac cactgccatg
     4921 atcccttgcg gagttggtag atctgaaaca gttgccatca cttcttctga tactagtgta
     4981 acagaaagcg cctgattttc ttcaagccaa ctagcccact cagtcagacc gcgttgcgta
     5041 ataaaaagtt cgttgacctt cgcatttgag gcaacagcct cctcgatcag gtgaaaccct
     5101 tcaagcagat attccccttt ttcttcacga tattttttct tatgtaattt ttttaattct
     5161 ttgattcttg gattttttgc tgattggatc tctttcacat ttttcacctc aactgattat
     5221 accatggatt agatcattgc acttgttcag tagcaaaaaa gtcggtatca ttaatttaaa
     5281 aataaaagaa agaacggtga ataaaatgaa caaaatcaag atgaacgtgc aaggacgtgt
     5341 acaaagcgtt ggttttcgct atatgacaaa aatcgtagcg gatcaattag ggatcaccgg
     5401 aacagtgaaa aatgaagatg acggctctgt cacgatcgaa gctgcgggcg acgatgaggc
     5461 aatcaaaaaa ttcgtggaag aagtcaaacg atcaccaagc cccagcggac gtgtccaata
     5521 tgtagacctt caggaaaatc cgatgatgga ggacagaaaa aagtttgttg taataggctg
     5581 accttctaga aatgaatagg aaatgtcaga aaaatcggac tgtctgtatg tagatcgaaa
     5641 aagtttgccc ctctctccat cctagatcga caaaaaaaca ttcttattag cgagtttagt
     5701 aaccagtgat aggcatacgt aaaagcggga ggacttcaaa aattttggtt caataaaatg
     5761 gagtctataa tattttatgg tagtggaaaa tcgactttga ttttccacta ccactttttc
     5821 attgtcaaat cctgacgctg cccagtcaac aagccaactc tcgaaaggat cattttccct
     5881 ctttgttcta atggataccc atagctgttt tcaaatatac agttttatct aaaaatttag
     5941 ttttgctaaa cgatcaaagg cttcacctaa gatttccaat ggctgagtcg ctgctagtcg
     6001 aatatagttg tccccgatcg tttccccaaa cgcttttcca gggacaaaaa gtacgccgct
     6061 tttttctaaa acctcttcca caaacgttac tgaatcgagc ccactcttac tgatattaat
     6121 aaaagcatac atgcttccag aaacagaaga taaagataag taagggattt ctgccactct
     6181 tttctcaata taagctaatc gttctttgaa tacttcaaca actggcggta caagctgttc
     6241 atagtgcttt aaggcgtaga ttgcacctct ttgagaagca gtcggtgcgg aataagtgat
     6301 gttttcattg atcaacccag caatttgatt gatgtatgct ggtgcgatca tgtaccctaa
     6361 gcgccagcca gtcatcgcaa accccttcga tacaccactg acagtgatcg tattatcggg
     6421 cgcaaatcgc gccatcggta cgaacttctc tttaaaggca aagtcttcat aaatctcatc
     6481 ggaaatgata tagagttcat tttcgattgc gacttcagcg atttttttca acgtgctttc
     6541 agaaaaaaca gctcccgtag gattattcgg cgaattgatg acgatcacct ttgtacgatc
     6601 cgtaatcgct gcttttaaat cttccacatc caactggaag cccttttctt cataggtagg
     6661 aacaaaaaca ggcttgcctc cagataaaat gacttgttcc cgatatggtg aaaaataagg
     6721 ttcatggatg atgacctcat cctctggatc tagtaatgcc tgtaaaacta gatacatccc
     6781 gtgcattgct ccgactgtag ctcgaacttg gttgcattca acgctcaatc catatttctt
     6841 ttgataaaaa tctacgaccg ttgacaagaa ttctttgctg ccatcaggcg cagtataatt
     6901 agtagcacca ttttttacat ctgtataagc tgcttcaata atcgtcggat cagtgatcaa
     6961 gtcaggatca ccaatcgata aatcgataat atcctctttt tcctttgcca aaatagcaat
     7021 attcatcaac agattttctg ctggttgctg aaaccggcgt gcaatcttac ttttgtccat
     7081 cttacttcct ccatctttgt tacaacactt tgttcaagaa atcctgtgtt cgactatttt
     7141 gcggattttc aaaaatctct ttcggtgttc cctcttcttg aatgatgccg tcagccataa
     7201 aaatcacacg atcggcaact tctttagcaa aacccatttc atgagtgacc acgaccatcg
     7261 tcatcccttt tttagctaaa tcctgcatga ctgccaacac ttcaccgacc atttcaggat
     7321 caagtgcaga tgtaggttca tcgaaaagca tcacatcagg attcattgct aatgctcgag
     7381 caattgctac ccgttgttgc tgtcccccag ataaactggt aggataactg tccacttttt
     7441 cctttagacc aacttggttc aataatgata gagcatgttc ttttgctttt ttagggtctt
     7501 cttttttgat ttttacaggt gtgatggtca aattttcgag tacattcata tggggaaaca
     7561 aattaaaact ttgaaatacc attcccattt tttgacgaat ttgattgata tcgctgtcag
     7621 gggctgtaat gttttgtccc ttaaaaaaga tttcaccaga agttggttct tccagcagat
     7681 tcatacaacg tagaattgta cttttcccac ttcctgaagg gccaatgatc accactactt
     7741 ctccttcttt cacagaaaga tcaattgatt ttaagacttc attctcgcca aaactttttg
     7801 acagtttttt tacattaagc attcagaccc attctccttt caactaaagc taatatacgg
     7861 gaaatactga aggtcaacac gaaataaatc aaagagacca acacatatgg taggaatggt
     7921 ttgaagctag caccttgtac cacgcccgtt tgaaagatca attccgatac cccaatgacc
     7981 gaaacaactg aggactcttt gatgatcgtg acaaactcat ttcctagagc tggaagaata
     8041 tttttgattg cttgtgggaa gatgatgaaa cgcatggctt ctttctggtc cattcctaaa
     8101 gaacgtgcag cttccatctg tcctttgttg accgcattga tcccggcacg gacaatttcg
     8161 gctacataag cagcactatt caacgagaca gcaatacaac ctgctaccag tttcgataga
     8221 tccaatccca gtactcctgt tccgaagtaa acgataaaga tttgaactaa gagaggtgtt
     8281 ccccgtacgt attcgatata aataatggct aatgccttta aaaatttatt cttacccaat
     8341 ttcatcagtg caagcaaact accaaagacc attccaaaca atacacccac aaaagctaat
     8401 aaaatagtgt attttgttcc aatcaaataa tacggaccgt atttttgaag aaaggactga
     8461 tcatcttgga acatcaattg attcgcttgt ttttgataat ctttcagcaa gccgtcgtca
     8521 ttgatcgttt tgattgacgc attgacatga tttttcagtt ctggtgtatt ttttgacatc
     8581 gcaatagctg tggttttttc gccttgcgca aattttactg gagtcaaagc tactttctta
     8641 ttctgttggg catatgcttc tgcaactggt tgttccagca caacaccttc aactttttca
     8701 ttcagcaaat tattgatcag atcaggaact ttttgcaggg aggtcaactc tgcaccagtc
     8761 agttcatttt tcactaactc ctcttgtgtg gtttgctttt gcgccccaac ggctaagcca
     8821 gaaaagtcgg ctgtcgtctt aaatttattt ttgtcctctt tacgaacgag tactttttgc
     8881 tgtaccacga aatatgggtc agaaaagtca acttcttgta aacgttcttc agtcaccgcc
     8941 ataccagata taactaaatc gatcttgcca gttttcaacg cacctaataa tgcatcaaaa
     9001 ccaagttctt ctacttttag tttcacaccc atatcctttg cgatcttttc ggcaatggat
     9061 acatcaaaac caacgatttt atccttacca tccacattcg catggaattc atagggcgca
     9121 tagtctgcag ataggccaac gactaattct ccactttgtt ttatccgctc gtaaatttca
     9181 tctttttctg caccttgtac ttttactggt aaaaaaacca gtagaaacat cagcgccact
     9241 aataatatgt ttattccttt tttcatccat ttggccacct ttgaataata atgtaatcag
     9301 aaaagctata aaagaataaa tattcataaa taacttgcta atatactagc ataactatta
     9361 taataaaaaa agattaattt aaaaaaataa tggaagaagt tttttatatg acaaaaaatt
     9421 taattgttcg cggtgcccct caagagtatg aaataaaaga aaacgcatgg gattcactcc
     9481 ctgaacacct ttacaaaagg catattgaca acgttttgat tctccatgga aaaacatctt
     9541 ggaaagcagt aaaagatgtt tttccagatt tatcaataac aaaaattttt gaatattatg
     9601 gtggagaatg taccgatcca cttgctgaca gttttgtttc tttatgcaaa aagaaggatt
     9661 tcaatggtat tattgcagtc ggcggcggga aagtagcaga tctaggaaaa ttgattgcct
     9721 ataagacaaa cttaccaatc atcatcttac ctaccttagc tgcgacgtgc gcagcttata
     9781 ctccacttag tgtggtctat aatgaagaag gagtgatgct tcgctatgat atgttcccta
     9841 aagcaaatga tttagtgttg atcgatccaa agatcatttt gcactcccct cgcgagatga
     9901 tgattgccgg tattggtgac accttagcaa aatggtatga atctcacgca atcatctctc
     9961 agttaactat caagcctatc gaagtccaag tagcggaatt tgcagcgtta cgatgccgcg
    10021 aaatcctttt agagaaaagt gcagaggcaa tggaagcgat ggaccagcaa aaaatcaatc
    10081 aagcttttat cgatgtcgtg gaaacaaaca ttttattggc tggtatggtt ggtgggtttg
    10141 gcgatgacta cggccgaaca gctggagctc attccattca cgatgcgatg accgtactcc
    10201 ctgatagcca tcagcaactt catggaaaca aagttgcata cggtatcctt gtccagctga
    10261 tgatcgaaaa taaattagca gaaatcgaac acctactgcc gttttatcat cacttgaact
    10321 taccaacgag tttagctgat atgggtttac atttaacaga aagtgactat caagccgtag
    10381 ctgaacgtgc atgtttacct gatgaaatga tccacttcat gaaagaaacg atcacaccag
    10441 cactggttgt ccagaaaatg aaagagttag aacaagtgac acagggatag ttagcccctt
    10501 ataccatggc agtcagcttt tttatggatc ggctcaatgg taaaaaaaaa gaagtggaaa
    10561 aaattgacat tccacttctt ttttttgcgc taaaattatg atttcaagga aatatccgtt
    10621 gctcatctta ttttagagaa cggaggtgct aagtatgctt tatactgaag tatttttagc
    10681 tggattcttc tttttaacat taagcgtctt ttttgatcct ccaatcggtt atttctatga
    10741 aatttctatg aagttgattg aattatgagc gcttttttag tatagttaag cttgtgtaaa
    10801 aattataaaa gaggaagtat tttatgaata aatggaaaaa ttggttattg ggctctgggc
    10861 ttgtcacgat catgcttttc ttgtccggtt gtgtccgcat ggattcaaat ggtaaccctg
    10921 acacttcagg aatcatttac cgtatattgg ttcacccaat gggacaagcg attacatatt
    10981 tagtggaaaa ctttaattgg tcctacggct gggctgttat cttgatgaca gtcatcgttc
    11041 gtatcatcat tttgccatta ggtattagtc aatcgaaaaa aacaatgatc caatctgaaa
    11101 agatgcaagc attgaaacca caagttgaag ctacgcaaca aaaattgaaa gctgcgacaa
    11161 ctcgagaaga acaaatggct gcacaagctg aaatgcaaca agtttatcgt gaaaatggct
    11221 taagtatgac tggtggtatc ggttgtttac cgcttctgat ccaaatgccg atcttttctg
    11281 cgctatattt cactgctcgc tatacagaag gcatccgtga atcaaccttt tacgggatcg
    11341 acctaggtaa ccctagctta gttttagtag cgattgccgg tattgcttat ttacttcaag
    11401 gctatatctc aactatcggt atccctgaag aacaaaagaa aacgatgcgt acgatgttga
    11461 tcgtatcacc cgctatgatc gtcttcatgt cgatcagtgc cccagctggt gtcactcttt
    11521 actgggtagt cggtgggatc tttagttgtc tacaaacatt cattacgaat gtcatcatga
    11581 aaccaagatt aaaagcgcaa gttgcggaag aattaaaaca aaatccgcca aaacaagtgg
    11641 tgacgcctcg taaagatgtc acacctgctg aacctgcgaa gcaaacaaaa aaacaagtac
    11701 aagcaccaaa aaacaatgcg aagaatggtc ggaatgctgg taaacagcaa aagcgtaaat
    11761 agttatttgt aactagcttc ttcgatcgac ccagatcatt tctgatttta tgacgataaa
    11821 agccaacgta ctgtgaaata tagaaaacta tatttcgcgg tacgttggct tttttataca
    11881 cggcctcttt atacatccgt atttatatcc agttgtatca ccagtcaaga agttgtgctt
    11941 gattattctt cttcatcgtc tcccatgcca gcaatcactt ggttcactga aacgatactc
    12001 tcacgacccg aaccaataaa atctggttct gctccaatca tttgcgaatt gatgaattcg
    12061 atgaccatcg gcaaattcat gccagcatat agttcgatcg tttcaccttc catgattagc
    12121 ttggaaacgg tatttgcagg tgtcccaccc aacagatcgc aaagcactac gtaatctttc
    12181 aatggtgctg taacggagag gaatctttct ttatacgtgt ccgcgccatc ttcaggcaat
    12241 aaagggatcg tatagatatt ttcttgtggt cccatgatca tttctgtact gttttttagt
    12301 tcttcacaaa atttaccatg actaatcaag actagttgtc tactcatctt tccaattctc
    12361 ccttatttcg aaatcacacc taatgcagat aatgctacac atacgatcaa tacgatgaat
    12421 attgctcgag taggtgtcat atttttctta cctaacatcc aataaacgcc accaacgatt
    12481 ccagctggta aaagtttagg taagatcata tcgatatagt tttgcatatt caacgttaca
    12541 tccccaatcg ttggtgccca tgagaattta acattgatca tggttgctac taacgctcca
    12601 accatgaaaa ctcctagtaa agtagccgca tctgtcaatg cagtcaagcg tgattgcatc
    12661 gtggtaacta gagaaatccc ttctttgtag gcaaattcta gttgtttcca acgaaagacc
    12721 atgacagcga cctgagcaag gatccagata aggataccta ctggattacc atctgttgcc
    12781 atatttgcgg ctaaagctcc aaatatggtc gggatcaatg ccgcaaatac agaatcacca
    12841 attgcagcga aggaacccat caatcctgct ttcatcccag aaacagtttc ctttgaagag
    12901 actccttctt tttcttcgat agcaagatcg atcccagtaa tgatcgtgtt aaagaaatta
    12961 gacgtattaa aaaattgcgt atgtgttttc atcatttctt ttaattctgg tgtatcatca
    13021 ccatagattt ttctaagttg tggcaagata gtatacagat aacctgaacc ttgcattctt
    13081 tcatagttcc aacctaattg gaaagtaaac aaacttctac gattgatctg cttaaaatct
    13141 tctttggtca atttataatt agatttcgtc atcttcgatt tccccctcat cagattgtga
    13201 ttgctgactt actactggat gttggtcatt cttttgatca ttcttataac tgatcgttgc
    13261 cagtgctaga ccaatcaatg ccacagcaag catcggtaat gaattgaatg ttcctgggaa
    13321 atctttgacc acgctagcca cacttgttcc taacaattgg acattcgaga acactgttgc
    13381 taataacgct gtgatcgtaa atcctaaaat cagataagaa atgtgtttct ttactggtag
    13441 gtagcgtagt aaaattgcaa acccaactgc tggtaagact gcccctgcaa cagataagcc
    13501 atctcctagc cattttaggt cgccgtttaa aacagccaca acattttgga ctaagccacc
    13561 accgaatgct agggccaaga atacaggaac cattctggaa agtgcccaag gtaatgcccc
    13621 catcaagaag ttccgttcaa ttgctttgta attcattgtt tccacatgtt gatcgatccg
    13681 atgtgcaaaa tacgtattcg caaaacgtgc caaaatatct aattggatca tcaaacttgc
    13741 tacaggaacc gcgatggccg caatcgcttg ttctgggttc atccctagag aaatggagaa
    13801 agctgttgct aagatcgttc cagagttggc atcgattttt gatgcaccac caaatgtccc
    13861 tacccctaaa acagtcaact gcatacttcc accaatgatt aaacctgctt ttaaatcacc
    13921 cattactaac ccagcatata atcctgcaaa aactggggcg ctcattgaag aataaatctg
    13981 taattcatct aaaatttgat accctgcata taatgttaat aataatatct gccaccattg
    14041 aatatccatg aatcattcct cctatttgat taaaagactc ataaagtctt ctgctttgtc
    14101 atttggcacc atttgagcga tcaaatgaac gccaagctga tctaatttct tgaagacctc
    14161 tacatccgca tctacgacat tgattgattt tgtgatggaa cgtgtttcat ctgtttgtga
    14221 catattgccg acatttaatt ctttgatcgg tacacctttt tctactagcg ctaagatacg
    14281 atctggtttt ttcgctacga tcagcactcg ttgagaatca tatttacctg ctaaaatatt
    14341 tgcggctgct ttttcaatag gtaaaatact taatttgact ccagctggcg ttgccaactt
    14401 taacccactt ttttcgatcg catttgttgc tacctcatcg tctacgacca tgatccgacc
    14461 aatattcaac tttgtcgtcc aaagatttgc cacttgtcca tgtatcaaac gtccatcaat
    14521 tcttactcca ataattgcca tcatccattc tccttttcta acttttttct attggttctt
    14581 tgacaaaatc tataacttca ctgaatctac cttctgtttc aaagataacg atttcatttt
    14641 gtccttttct aaataaaccc tcgggaacaa acagtgaaag aattggtcct acttcccaaa
    14701 aacgaccaat attcgtgcca ttcacccaaa cgatcccttt cccaaactca ctaaggtcca
    14761 aataagtatc accgatttct gacagttccg cttcaaagcg gtaaaatgct ggttgacctg
    14821 cttcccattt ttttgaaaaa tcgatgtgtt ctgtagtctc taaaggtaaa caatactgtg
    14881 tccattctgt gataaaatga agatcgctca tcactcctgt acgtattcct tttctttgcg
    14941 tatcagctag cagcttgtgt ccatagttga cccgtcccat attttcaaat aatagatcaa
    15001 tttgatggtc ttctttctca agtgtcaatg gaatggtctc accgatctct gtttggtatt
    15061 gaatcgcttg cttttcttca tcgagaaaaa catggacccg atcacgtccg tcgatgatcc
    15121 gtaatttttc attgaccgca tcttttttga tcgttgttcg atacaatagg tacccagtat
    15181 tctgacccaa ttcttccatt gtttgaggat aggtcgagtg gatcggcttg ctgatggtat
    15241 ccagtgttgc gaaaaagctg actttttctt tcaagttgat tccttttttg acgattgttt
    15301 cttttatcca tggttccatt tgctgcattt ctggaaaatt ttctgccatc attcgtttca
    15361 gagcaaaata tttttccgtt ggatttccct gctcatccaa tggtgcatcg taatcatagg
    15421 aagtgacttg cggcaaatca atcgcgcctc tggctgaaca gccattcata aacccaaaat
    15481 tggttccgcc atggaacata tagagattga tactgccgat ctgtaatgct tctttgactg
    15541 catccgctaa ttcttccgga tctcttttaa tgatcggctc tttccaacga ttaaaccagc
    15601 catcccaaaa ttccatacac atcaacggcc attttttacc atgtgcttca aaaaactggg
    15661 ccatatctgc aaaattttct tttgcttttg agccaaaatt ccctgtcgta aaaacatcat
    15721 cgtcaatcaa aatgcctgct cttaatgtgg cttgccaagg tccatcagaa gtgaatagcg
    15781 gtacatcgat tccaccttcc tccaagtatt tttttacttg gcgcaggtat gctttctctt
    15841 caccatacga accgtactca ttttctacct gcaccattaa gacgttgcca ccatgagtga
    15901 ctagatgagg aacgatttta ggtagtagcg tttggtaata ccgtttgaca tacttcatga
    15961 agcgaggatc cgctgaacga atacggcact cctttgtcaa tagccaagca ggcaagccac
    16021 caaattccca ttctgcacaa atataagggg ctggacgtac gattgcgtat aatccaaggg
    16081 attgcgctaa ctctagaaaa tgggaaagat ccaatatttt cgaaaaatca taggttcctt
    16141 cttctttttc atggagattc caaggcacat aggtttccac agtattaaat ccaagcgctt
    16201 tcaaattgtg caatgaccgc tcccaattcg tcggatgcac tcggaaataa tggatggcac
    16261 cagataaaat tttgaaaggt tgaccgtcta acaaaaactc ttcttttatt tcaaatctag
    16321 tcaagcaaat ttcctcctcg tttttagaaa ccgctttcta attttataat caacgaatct
    16381 ttctgacgtt gatttccccc ctaaaataat cgactttcac aggatcgatc catcctgttt
    16441 tttcttgttg tgcattcgcc atcccacagg ccatcgcaaa cgcaagtgtt tcttcaatcg
    16501 tatagctctt tgatagagca tgtgctaagc ctgcaatagt cgaatctcct gaccccacag
    16561 gattcaccac cgcgatttta ggaatcgtaa cctgatagaa gtgtctccct actttagcga
    16621 ttgctccttt tgcgcctagc gaaagaacga tccaattgat tccttcaaaa agcggatgat
    16681 ccaataatga ttggatttca tctaacgaag tagccgaaga aattgtttgg tggaccaact
    16741 ctcctaattc ttccaaatta ggtttgatca aaaatggttt ttccttgcct tccaagactt
    16801 caagcaaggt ttgaccagaa gtatctaaca atactcgtgt tcctttttta ttcgttaggg
    16861 caagtaactc tgcataataa cttttagaaa atccttgagg cagactaccc gaaatcgtaa
    16921 agatatcagt tgtttccatt agtcgtgcaa aatgctcggt aaatttatgt tgctcttctt
    16981 ccgtcacaac aggaccgctt tccaatagtt ctgtttgatt cactccatgt aaaatagcaa
    17041 tcgaatctct cgtttcccct ttgataggta aaaattgatg cgttatccct gattgatcga
    17101 gttgttcttt gatatatgct ccatgctgcc cgccgactaa tccagtagct aaaacatcat
    17161 ctgctaataa atgaaggaca cgagcaacat tcaagccttt tcctccagcc gttttgttga
    17221 ccgttttgac ccgattgact tggtcgatcc gtaattcatc caacgtgtaa gcgatgtcaa
    17281 ttgaaggatt catggtaact gtcgtgatca ttttgctgtc accgaatctt cttttacttt
    17341 gtcaaaccaa ggggtggcgt tttcatttat cactttattc aacgattcga tattttgtcg
    17401 cccttgtgtt ctcaaccact caatcgcacc ttctctacca ttttcaacat atgctttgac
    17461 tccatctttc caagtcgcac gtccgcataa cacaccgttg aatgttgaac cagattcatg
    17521 agcaaagatc aatgtttgtt ggaacaatgc agtcgaaacc cctgcactca agaagataaa
    17581 tggtaacggt gttgcatcac tttgttcctt gaagaatgcc gctgcttctt ttttactata
    17641 cgcaatttca ccatcagaat aaccttctac atagttcata ttgactggca cttctacttt
    17701 caatacatcc actttatatt taggatcaga aaattctttc atcatctcga tcactttatg
    17761 cggtttgatc ttcgcatatt ctaatgaagt gctgtcattg gttttggcat catacgaaac
    17821 caattctaga taaaacggac gcttttcacc tgcacattca ctgcctaaac gttcgataaa
    17881 tacatgtttc aaatgattga tctctgggtc ttcatctaca tcataataaa gcaaaaactt
    17941 gatcgcatcg gctgtttgtt cctttaatct aagcgtagac caatccgcta ataaatcagg
    18001 cagacgtcct ggagttgtcg catcataacc cgttttttca taggctaaaa gtagtcctgc
    18061 ttcctgatgt ctggctttag cagctggcaa tccatactct ggatctagta aaatagcaga
    18121 agcataaggc gttaattctg ctgatactaa ttctttgaat tcagtgatct gttgcgccgt
    18181 tggttctttt cctaacgctt tgatcatctt tttcaaagca ccgcgttgat caattgccaa
    18241 tgcactgatg attccttctt ttgttgataa actcttcata gctgcaattt tttcttcatt
    18301 catccttcgt ttcccctttg ttcacatatt catggataat gacacctttg accactcgat
    18361 tgactgtacc tgtaggcgat ggtgtatctg gtttattcgc tacttttatc gacgtatcga
    18421 gcgcaatgat ttgggctgtc atgatcatag gtaaggcaag atacgcatca ggtaggctga
    18481 tcgaagcttc taacaaaaga cttgtacctg tgaaatcgat gtttcctggt tgggcgatcg
    18541 cgatgacact cgtggtaatt tggtcatctt ttatttcttc tagaatatct aagtcataat
    18601 ttcttgtata cggttggtta ttaacaaaac caataacaag tgtttcgcta tttaaaaacg
    18661 attttggtcc atggcggaag cccatagaag aatcaaagat cgttgccact ttaccggctg
    18721 tcaattccaa tattttcaat tgtgcttctc tcgttgcacc agccaaactt cctgaaccaa
    18781 gataggcgat acgttcaaat gacttaggca ataatttttg gatttcttga tctcgtgaaa
    18841 ttagatcttc tccagttctg atcaattgtt gaagataact ttcttttttc tcaacggcag
    18901 tttggtcgaa tacccataac gcactcaaca acatgcacgt aaaacttcct gtcatggcaa
    18961 atcctgcatc atttgatttt tctggcatca gtaaaagata atttcgttca tctgtcgctg
    19021 cattctttgc taactgacct tctttagcac aagtaatcgt caaatgaaac aatgtcttga
    19081 tttgttcatt ggcaatcttg actgttgcca cactttctgg actatttcca ctacgtgcaa
    19141 atgacaccag taatgtcggt tcatccggaa atagaaattc ttttggtgaa gacacgatat
    19201 ctgtcgtacc aaagctatga aaaataaagt gatcgttatc accgtattca tttaaataag
    19261 gtactgctgt atccccgaca tattgtgatg tgcctgcacc agtaaaaatc actcgaactc
    19321 ttttgccatt tgctgctttt tttatctggt caaaaaacaa tgctaatttt tcttgattct
    19381 gtgctaatat ctcagctgtt tctttccata attgcggttg ctgtttgatt tctcgcgtag
    19441 ttatttccgc acccaactct tttaatttat ccttcgatag ttcaaacatg ttgttttccc
    19501 tctcaatcat tttcggacgt ggcggatctt ataatgaaac tggtctgctc tcgctacact
    19561 atccgtatat tcaataattt catttttcac attataggtc gttcggctta gatataatac
    19621 cggggcacca tcagcgatac gaagcggatc agcatctttc ttttgaacga tacttgcata
    19681 aaattcttca tctgctaatt ggacttgctg ttcataatcc tgagaaaaca catcatacaa
    19741 tggcttactt tcaagtaatg gcagatcaag accaataaat agttttgcag gtaaataaga
    19801 acgttccacc atcaatggtt cgtcatccgc taaacgaagt cttttcaatt tgaaaactgt
    19861 ttcgcctaaa ttgatgttta gattcttggc aacctttttg tttgcttcca tttgcttgaa
    19921 ttccaagatg actgtattag gcacccgtcc caattccctc atctcttctg taaaactata
    19981 cgctcccgcc agatcagacg acttatctga tagatcagac acatatgttc cttttccgtg
    20041 tcggcgataa atataccctt gcgtctctaa ttcttgcaaa gcaaggcgaa ccgttgtccg
    20101 actcactcca taatgctgac aaagttctct ttcagataac aatttgtcat gaggtgtcat
    20161 ctgtgtttcg atacgttctt tcaatacgtc aactagttga taatataatg attttccttt
    20221 acttgagctc tccataaaaa tacctcttct ttactggtta taaccacaac acaagaatac
    20281 ccacatattc ctcatttgtc aaagggtttt tggcgtaaaa aagctattat aacaaggttt
    20341 caaggtaatt tagaactggt atcaaaagat acctcaaaaa taccagttga caaataaaaa
    20401 acaaaaaaaa gctgaatctt ggcaaagatt cagctaaatg gtctagagaa gtaaagaaaa
    20461 aatattcttt atacatctgt ggctatttct actatcgaca agcaaatggg cctaatagac
    20521 tactatgtaa ataaaaatgg ctttctctaa ataaagtagg attttttatt ttttagataa
    20581 cttttctctc atgccggtct gatagtcgaa attttgttgt acatttttaa aaataacaaa
    20641 aattttttat gataattctg tctttttcta gtttccttat ggcgtaagtt taacgaataa
    20701 gaatttagta tctatacttt tatattacaa aaaatattcg taaaattcgg gctct
//
LOCUS       DAZAJZ010000049        19962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000049 DAZAJZ010000000
VERSION     DAZAJZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.049"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..1566)
                     /locus_tag="ACG86D_001706"
     CDS             complement(541..1566)
                     /locus_tag="ACG86D_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3140844.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="ACG86D_001707"
     CDS             1841..3526
                     /locus_tag="ACG86D_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN3140845.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="ACG86D_001708"
     CDS             3540..4823
                     /locus_tag="ACG86D_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140846.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="ACG86D_001709"
     CDS             4893..5762
                     /locus_tag="ACG86D_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140847.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="ACG86D_001710"
     CDS             5759..6595
                     /locus_tag="ACG86D_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3140848.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="ACG86D_001711"
     CDS             6621..7688
                     /locus_tag="ACG86D_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN3140849.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="ACG86D_001712"
     CDS             7685..8512
                     /locus_tag="ACG86D_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3140850.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="ACG86D_001713"
     CDS             8557..9582
                     /locus_tag="ACG86D_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3140851.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="ACG86D_001714"
     CDS             9579..11762
                     /locus_tag="ACG86D_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN3140852.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="ACG86D_001715"
     CDS             11762..12892
                     /locus_tag="ACG86D_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3140853.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="ACG86D_001716"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="ACG86D_001716"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN3140854.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="ACG86D_001717"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="ACG86D_001717"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3140855.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="ACG86D_001718"
     CDS             complement(14378..15064)
                     /locus_tag="ACG86D_001718"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN3140856.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="ACG86D_001719"
     CDS             15144..16682
                     /locus_tag="ACG86D_001719"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN3140857.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="ACG86D_001720"
     CDS             16802..17269
                     /locus_tag="ACG86D_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN3140858.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17803..18231
                     /gene="rplM"
                     /locus_tag="ACG86D_001721"
     CDS             17803..18231
                     /gene="rplM"
                     /locus_tag="ACG86D_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN3140859.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="ACG86D_001722"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="ACG86D_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN3140860.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..19953)
                     /locus_tag="ACG86D_001723"
     CDS             complement(18727..19953)
                     /locus_tag="ACG86D_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3140861.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
BASE COUNT         6443 a         3600 c         4199 g         5720 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggtta attcatctga
    19561 tattatctca gtattgatat ttagataatt cattgcgtat tcaccaaaca taggtaaaat
    19621 atgcaaccga aatatatctt ttgtattact aatggttata tcactaggag gcctgtttga
    19681 tctaattgtt tgacctaatt catatctagg aagccattta gattcgtaaa attttttaaa
    19741 cgttattttt ccttttaatt ctaatgagtt tgcattctcc tctctcaaaa ctttttctat
    19801 tttcttcttc atatcatttt cggctttaat cgccaattgc ctatttggaa atgttttttg
    19861 atacttttct gatgcaattc ctgttatttc tttaaacctt tttggatatc tcagattagt
    19921 tttatatttc ccatttgaaa gctttgtagg caaaatcctc ac
//
LOCUS       DAZAJZ010000050        19629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000050 DAZAJZ010000000
VERSION     DAZAJZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.050"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG86D_001724"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG86D_001724"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN3140862.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG86D_001725"
     CDS             complement(1172..1867)
                     /locus_tag="ACG86D_001725"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN3140863.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG86D_001726"
     CDS             complement(1880..2596)
                     /locus_tag="ACG86D_001726"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN3140864.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG86D_001727"
     CDS             2926..3534
                     /locus_tag="ACG86D_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN3140865.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG86D_001728"
     CDS             3859..4182
                     /locus_tag="ACG86D_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN3140866.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG86D_001729"
     CDS             4364..5734
                     /locus_tag="ACG86D_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN3140867.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG86D_001730"
     CDS             complement(5864..7165)
                     /locus_tag="ACG86D_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN3140868.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG86D_001731"
     CDS             complement(7519..9510)
                     /locus_tag="ACG86D_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN3140869.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG86D_001732"
     CDS             complement(9654..9920)
                     /locus_tag="ACG86D_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3140870.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG86D_001733"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG86D_001733"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN3140871.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG86D_001734"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG86D_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN3140872.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG86D_001735"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG86D_001735"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN3140873.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG86D_001736"
     CDS             complement(14080..14601)
                     /locus_tag="ACG86D_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN3140874.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG86D_001737"
     CDS             complement(14604..15446)
                     /locus_tag="ACG86D_001737"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN3140875.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG86D_001738"
     CDS             complement(15439..16164)
                     /locus_tag="ACG86D_001738"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN3140876.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG86D_001739"
     CDS             16340..16636
                     /locus_tag="ACG86D_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140877.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG86D_001740"
     CDS             17204..18796
                     /locus_tag="ACG86D_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3140878.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG86D_001741"
     CDS             18809..19549
                     /locus_tag="ACG86D_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3140879.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAZAJZ010000051        19475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000051 DAZAJZ010000000
VERSION     DAZAJZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.051"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACG86D_001742"
     CDS             127..3654
                     /locus_tag="ACG86D_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN3140880.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACG86D_001743"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACG86D_001743"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN3140881.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACG86D_001744"
     CDS             7442..7783
                     /locus_tag="ACG86D_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN3140882.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACG86D_001745"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACG86D_001745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN3140883.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACG86D_001746"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACG86D_001746"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN3140884.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG86D_001747"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG86D_001747"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN3140885.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACG86D_001748"
     CDS             12994..13731
                     /locus_tag="ACG86D_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3140886.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACG86D_001749"
     CDS             13745..14560
                     /locus_tag="ACG86D_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3140887.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACG86D_001750"
     CDS             14573..15220
                     /locus_tag="ACG86D_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3140888.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACG86D_001751"
     CDS             15236..15892
                     /locus_tag="ACG86D_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3140889.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACG86D_001752"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACG86D_001752"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN3140890.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACG86D_001753"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACG86D_001753"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN3140891.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACG86D_001754"
     CDS             18234..18752
                     /locus_tag="ACG86D_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3140892.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG86D_001755"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG86D_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN3140893.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5397 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaagg
//
LOCUS       DAZAJZ010000052        19389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000052 DAZAJZ010000000
VERSION     DAZAJZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.052"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACG86D_001756"
     CDS             complement(<1..209)
                     /locus_tag="ACG86D_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140894.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDI"
     gene            complement(300..752)
                     /locus_tag="ACG86D_001757"
     CDS             complement(300..752)
                     /locus_tag="ACG86D_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN3140895.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(765..1175)
                     /locus_tag="ACG86D_001758"
     CDS             complement(765..1175)
                     /locus_tag="ACG86D_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3140896.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1450..1662
                     /locus_tag="ACG86D_001759"
     CDS             1450..1662
                     /locus_tag="ACG86D_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFN3140897.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1665..1877
                     /locus_tag="ACG86D_001760"
     CDS             1665..1877
                     /locus_tag="ACG86D_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140898.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2017..2172
                     /locus_tag="ACG86D_001761"
     CDS             2017..2172
                     /locus_tag="ACG86D_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140899.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2169..2393)
                     /locus_tag="ACG86D_001762"
     CDS             complement(2169..2393)
                     /locus_tag="ACG86D_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN3140900.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2471..2659
                     /locus_tag="ACG86D_001763"
     CDS             2471..2659
                     /locus_tag="ACG86D_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140901.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2798..2926
                     /locus_tag="ACG86D_001764"
     CDS             2798..2926
                     /locus_tag="ACG86D_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140902.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2985..3320
                     /locus_tag="ACG86D_001765"
     CDS             2985..3320
                     /locus_tag="ACG86D_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140903.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3553..4494
                     /locus_tag="ACG86D_001766"
     CDS             3553..4494
                     /locus_tag="ACG86D_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFN3140904.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4496..5386
                     /locus_tag="ACG86D_001767"
     CDS             4496..5386
                     /locus_tag="ACG86D_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN3140905.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5429..6229
                     /locus_tag="ACG86D_001768"
     CDS             5429..6229
                     /locus_tag="ACG86D_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN3140906.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6244..7095
                     /locus_tag="ACG86D_001769"
     CDS             6244..7095
                     /locus_tag="ACG86D_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3140907.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7296..7757
                     /locus_tag="ACG86D_001770"
     CDS             7296..7757
                     /locus_tag="ACG86D_001770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3140908.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7783..8235
                     /locus_tag="ACG86D_001771"
     CDS             7783..8235
                     /locus_tag="ACG86D_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140909.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8341..8535
                     /locus_tag="ACG86D_001772"
     CDS             8341..8535
                     /locus_tag="ACG86D_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140910.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8566..8952
                     /locus_tag="ACG86D_001773"
     CDS             8566..8952
                     /locus_tag="ACG86D_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140911.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8949..9152
                     /locus_tag="ACG86D_001774"
     CDS             8949..9152
                     /locus_tag="ACG86D_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN3140912.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9149..9445
                     /locus_tag="ACG86D_001775"
     CDS             9149..9445
                     /locus_tag="ACG86D_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140913.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9521..9937
                     /locus_tag="ACG86D_001776"
     CDS             9521..9937
                     /locus_tag="ACG86D_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN3140914.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10198..10335
                     /locus_tag="ACG86D_001777"
     CDS             10198..10335
                     /locus_tag="ACG86D_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140915.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10745..10975
                     /locus_tag="ACG86D_001778"
     CDS             10745..10975
                     /locus_tag="ACG86D_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140916.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11134..11967
                     /gene="terS"
                     /locus_tag="ACG86D_001779"
     CDS             11134..11967
                     /gene="terS"
                     /locus_tag="ACG86D_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFN3140917.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11960..13375
                     /locus_tag="ACG86D_001780"
     CDS             11960..13375
                     /locus_tag="ACG86D_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFN3140918.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13387..14916
                     /locus_tag="ACG86D_001781"
     CDS             13387..14916
                     /locus_tag="ACG86D_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3140919.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14834..15886
                     /locus_tag="ACG86D_001782"
     CDS             14834..15886
                     /locus_tag="ACG86D_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFN3140920.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15888..16205
                     /locus_tag="ACG86D_001783"
     CDS             15888..16205
                     /locus_tag="ACG86D_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140921.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16332..16955
                     /locus_tag="ACG86D_001784"
     CDS             16332..16955
                     /locus_tag="ACG86D_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFN3140922.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16970..17860
                     /locus_tag="ACG86D_001785"
     CDS             16970..17860
                     /locus_tag="ACG86D_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFN3140923.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17883..18098
                     /locus_tag="ACG86D_001786"
     CDS             17883..18098
                     /locus_tag="ACG86D_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140924.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18110..18451
                     /locus_tag="ACG86D_001787"
     CDS             18110..18451
                     /locus_tag="ACG86D_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140925.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18451..18822
                     /locus_tag="ACG86D_001788"
     CDS             18451..18822
                     /locus_tag="ACG86D_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140926.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18815..19210
                     /locus_tag="ACG86D_001789"
     CDS             18815..19210
                     /locus_tag="ACG86D_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFN3140927.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
BASE COUNT         7054 a         2867 c         3967 g         5501 t
ORIGIN      
        1 ttaatatcat tttttaacat agtaatttgt ttatttaaat ctgatagtgt ttcttcctta
       61 cttaatataa atctttctct atcatgtaat ttatttagtt tttcatcatt tttttgttct
      121 aattcataag cttctttttt tattgtgtta gctttcgaca gtttagtgat tcctataatt
      181 aataaaataa gtccaatcaa taataacatt caaaactcct catttctttg atataattat
      241 tgtagagttt actcagaaat gaggaaagag tccatgtgaa agcatgggct tttttatttt
      301 taatgttgta atactaaatg ttcaagatta tgaggaagcc ctaaatattc aataatttgg
      361 aactgactca tatcagataa atcttgttca tctattgttc tagttaacat ttcaattgca
      421 aacatattag cttcagcttc tataccataa atcatacaac taaggttgtt tgctctaaaa
      481 gctgctgtgc aaataccttt atggagtcta acgtgggcta tttcatgcca taatacaaat
      541 cttttcatag aaggtgatag gttttgatta attaatatca cataacatct tttattgtgt
      601 atcgtttgtc ctaatgtttt attccctaaa tcagtttcaa ttaaatctat atcagaatat
      661 ttgattattt tatatggatt aatcgcatca gatccaaact ctacaaataa tttttctatt
      721 tcttctaata tccagtattt gtttaaatgc ataccaaagc ccacctattg acgatatttt
      781 ttaggagtga attttttctt tgctttaact ttggttaagg caagtgcaag ttgaatggca
      841 tcgtataatt tttccttatc ttcatcgtct aaaggttcac cataataatt tgtttcagca
      901 tctgaattca agccttcaag taatttttca gcttgtaaag caatatcttg tttatctttt
      961 tctgttaaat cataataatg gcgtttatca gtacgtccta aaagataatc agttgataca
     1021 ttaaagtaat ctgcaacttt ttgtatttta tctccagatg gagtactttt attccatcta
     1081 gacatgcttc catttccgaa atttaacttt ctttcaagtt ctgccaacgt cattgaacgt
     1141 tcttttaata agatttttat acggtctaat agcataggat atgcattcct ttcaaaatac
     1201 cgacaaaaaa agtatttttc tgcaaattat ctatttacaa atagagaatt tgcagttata
     1261 ctagttttgt aagcaagata tttacggcat acaaagacta aaagcaataa actaatagtc
     1321 tccccagaca tcagtaagct cgctttttgt gcgctgtatt ttgtatgctt acataatagc
     1381 atatttgcag tttttgtcaa acttattaga caaaaatatt agaaaatatt cgataagggg
     1441 gtatgaagca tgactttaaa acagcaagta ttaattgctt tagtaaagaa aggttggagt
     1501 caaagagagt tagctcgacg aatgggtata tcaattactt atttacgaga tatcttcatc
     1561 gaaaaaagaa aacctaagga acgtttaaaa caaatcgaag aacttttgga tatcaagtta
     1621 gaagtagatt ctaatgatca gccatcagaa caggaggcgt aaaaatggta caacgtttga
     1681 gcaaagcgga gtttaaagag aaatataagt attctgaaag tacttatcaa agaagaatga
     1741 aggaatttaa aaaatcacgt tttagtgagg ggtataacgc agttacttcc aaagaaataa
     1801 caattgatgt cgatttatat gaacagttta gaacatggcg ttctaaaaat cgatttagag
     1861 ttagagctgt ttgttaatag ctaaaaaatc taaattcacc acttctgaaa agaggagttg
     1921 ctgctctcct ttcaagtttg ccaaagtcca cgttttttca ttttggcaac tcctcttctt
     1981 ggaggtggtg acggcgagag acgaaggagg gatgacatga aggaagaatc aaaaataggg
     2041 attttgaatg agaacaatta cacaagatta attattgaaa cagaagaagg gaaaaaagta
     2101 gcagaagtta cattgaccga tgcaactcct gctgacggtt atcgtattcg attaactcct
     2161 aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt gactatcacg gctttgaatt
     2221 tttccatctc tgccatgaat aaatagctca gatccttgat ttttagaaat ctctctagcg
     2281 atttttactg cttctgattt tgtattagta tgtgcagtcg ctttagaatt tccagcacct
     2341 ttgacattcc aaccaccatt ggaattaggt acaacatgtt gattttttcc catagttttc
     2401 acctccattt ctatagaaat attttatcaa atagaaatgg agaaattcaa taaacgaaag
     2461 gagaagtgat atggcatata ccttgcaaca agaacatcaa attctcggtt tgattaaaca
     2521 acgcaggaaa caattacaag atgaccgtgc agcgcttaga aaagccgatg agctatcaga
     2581 taggcaagct gaactaattg cttctgaact tgaggatttg agaatgctag aaataaaaaa
     2641 tagggagatt agattatgaa gaagacagac acacttttta taggattcat tttggggtta
     2701 ttagtgattg tagcgcacca aagtattatc gggggaagct tgttcgcagc attgatggtt
     2761 ttaatcaatc tgcttgattc aaaagaaagg agcaactatg gcacgagaag aagcgctaaa
     2821 aatcggtaaa gtgattgctg ataatcggtg gggaaatagc cgccctatta ttttaagcaa
     2881 gcaacatatc gaaaagcaaa aagcatggca acaaataaaa aagtgactct gccagccagc
     2941 aaagagtcac atacaaattt ataaaactag gagaattata tcacatgaat aagaaaattg
     3001 aaaatcttat cgaagaattg aaacgtgaat gccaaaagca aggtgtttct attatttgta
     3061 cagcccaaaa agaaggggaa cttaaaagtc tcgtttatgg tgagacaaca gaaattttac
     3121 tttgtcttgc tatgcaagaa gaacatttag atgaaaatct cccattgtca gcacatatta
     3181 tgcgtagaat tgctgtggat gcttacgaac aagcgaaaaa tgaagaagaa aatcaacctt
     3241 caaatcatac ttttgttatt aataacaagg aagatttagc tgatgtgatg actcgtattc
     3301 tcaaggggga atttcaatga tgaatgaaaa aacaattgat ctactcatta ttttattttt
     3361 cacgttagtg attgtagcga aaagttatgt aatctttggc ttgttattcg caattaccat
     3421 gcttgtgctt tgcgtgttag caagcagaaa agaggttaaa catgaacgag ttagcaaatc
     3481 ttgacaacta tttaactgat cctgattatg ctaaaccacc atatgaagca ccaattgatg
     3541 aggaggatga agatgagtaa atctacctta gaaatgagcc atcaagaatg gcttgaagac
     3601 cgtaagaaag gcatcggagg ttcggatgtt ggaacaattt taggattgaa taaatggaaa
     3661 tctccttatc aactatggct agaaaaaaca ggacaagtag tacttgaaga atcaggaagt
     3721 gagcccgctt attggggtaa tgttttagaa gaagtggttg ctaaagagtt tcaggaacgt
     3781 acaggcaaaa aagtacgcag aagaaaccaa gtatttgaac atccattaca tccattttta
     3841 agagcaaata ttgatcggga agtagtggga gaaaatgcca ttctggaatg caaaacagct
     3901 aatcaatttc tcggtaaaga atgggaaggc gaagaggtac cgcttagcta tctctgtcaa
     3961 gttcaacatt acatgaacgt tctaaacaaa gactattgtt acatcgctgt cttaatcggt
     4021 ggtcaaaaat ttatctggaa gcggattgaa cgagatcaag agctgatcga tacaatcact
     4081 gaacaattag tagagttttg ggaaacaaac gttcttggag gtatcgagcc aattattgat
     4141 ggtagtcaag cgacagctga tttcttgaaa gagaagtatg cagatgttga agacgttcaa
     4201 acgacgttac caattcattt cgatgaacta gttgaacaga aaaacgaact caaacggact
     4261 aagaaagaaa ttgaatcagc tatccgtcaa gtagacaacg agattatcag cgagctaggt
     4321 aaacgtgaag ccagtatcgg tatcactcaa aaaaacatca tcagttggaa atttgtccgt
     4381 acgagacgta tgaactcgaa gaaactagca gagaaatatc cagatgtcgc aaatgatgaa
     4441 gagatttata acgttactga atcaagaagg ctaaccgaaa gggagatcaa ataatatggc
     4501 aacaaatgaa tcgttaaaaa atcaattggc agcaaagcca cagaaacaag ttgcaccagg
     4561 tcaattagga cttaaagctt tgatgaatac accaacaatg agaaagaagt tcgaagaggt
     4621 gcttcatgac aatgctaatg cttttatgtc gaatgttatg actcttgtat ctaatgacag
     4681 ttatcttgca gatagtgaac cgatgtctat catgagtggt gcgttaactg ctgcaacatt
     4741 aaatcttggg ctagataaga atttaggtta tgcatattta gttccattca atagtaaaaa
     4801 caagcaaaca ggaaaatggg aaaagaaagc tcaattcatg cttggctata aaggatatat
     4861 ccaattagcc caacgatcag gtaaatacaa agcattaaat gtgatcgaag tttacgaagg
     4921 agaactaaaa agctggaacc gactgacaga agagtttgag tttgatccaa atggtagaac
     4981 gtctgatgaa gtcattggat atgttggcta tttcgagtta ctgaatggat tcaaaaaaac
     5041 tgtctattgg accaaacaag aaatcgaagc tcatcggatt gctaacaata aagatcgcga
     5101 taagacaaag ttaagtggtg tgtgggcatc tgattacaat gcaatggcac gaaaaactgt
     5161 tttgagaaat cttctttcta aatggggaat cttgtccatc gaaatgcaag aagccaccac
     5221 atcggatgag agagtccaaa gggttcaaga agacggcagc attattgctg aaacagaagt
     5281 tgaagaagat attcctgaaa gaaaagaagc agaggttata tctgaagaaa atgaagatgt
     5341 acaaactgga ttatttgatg catctaatcc gccgttaaac aaataatgag ggagctttct
     5401 cccttgcttt tctagaggag aaatacgaat gaacacagga tatgtcaaat tacatcgaaa
     5461 agtgatgaat tcattcgttt ggaccaatcc ttatatgtat aaattatgga acttgtgttt
     5521 gatgaaagcg agtcatgaga atcgcaagat actttttaat ggaaaagaga tacaagtgaa
     5581 cagcggagaa ttcgtcacag ggcgcgatgc tatcacatct gagatgaacg aaggcgtgaa
     5641 acgtgaacat caagtgaaca gcggttctgt atggagatgg ctaaaacaat ttgaaaaaaa
     5701 cggaatgttg aacatcaaat caactacgaa atacagcgtt atatcaataa aaaactggtc
     5761 tttgtaccaa agcagtgaac aacaaatgaa catcaaacgc acaacaagtg aacaacaagt
     5821 gcacacaaac aagaatgaaa agaattataa gaatgaaaag aataataaaa catcatcgtc
     5881 gcaaccacgc aaaaagcgtg tttacgacac cgactcagtt tactacattc tcgcggagga
     5941 gttattcaaa cagatttgtc agaatcagga aatcaaaaag ccgaatctgc aaagttgggc
     6001 agataacatt cggaaaatga tcgagatcga caagcggacc gaaaaccagg tacggggaat
     6061 gattgaatgg agtcagcaca atgtgttttg ggcatcgaat attttatctg ctaaaaaatt
     6121 gcgagaaaaa tacgacacaa tggcagcgca agccaatcgt gattataaaa caaaacaaac
     6181 taaaacgctt gaatacgaga aatttagcac agatgagttg cctatttgag aggaggcgta
     6241 ggcatggaaa ctgttgggga aatcatggaa aagctgatac agaaagtgct tgtccaacgt
     6301 ggcgaatgtc ctgaatgcgg acagcctttg tatggatggc gtacgaagaa ccctgatggc
     6361 tcagaacgtt gtaagccaac ctgtatgcaa tgtgggtata aagcattgcg agttcaggaa
     6421 gacttacaaa cagaacgaat ttacaacgag agcctgaaag caagagcaat caattttttc
     6481 aaaggtggtt ctgttgtgcc taatcaagcg ttgtttgatt gcacattgca gaattatcaa
     6541 attgtcgatc aagaaacaag acaagcggtt gaagtaacca aacgctttgt taattcagtc
     6601 ttgttaggaa atccaagtca ccttgtttta actggaaaac aaggaacagg taaaagtcac
     6661 ctagcgatgg cagcggcttg ggaagtcttg aagcgatcaa actacgataa aaaaatctta
     6721 tttatcgggt tacaggaaat gctggatcaa atcaaatttt cgtacaacaa tcctgaactc
     6781 agaaaaacga ttgagggatc gttgattgca gatatcaaaa cagcggattt agtcatcatt
     6841 gatgatatcg gttcagaact aggaaaagat gcatcagata gtcgagcgtt tggcataaac
     6901 acgctaaatt cgttcttgga tgcacgacag aacctagcaa cgattatcac aacgaacttg
     6961 cttggtgaag aactgaaaaa agcttacggt acgagaacaa tatcaagaat gtttgtcaac
     7021 tctgatggat ttacgatggt attttctcaa acagcagaca agcgcataaa accagtcaaa
     7081 ggagcgatcg catgaatttg actaaacaag aaaaaagaat ctgtcgtcag ataatgttgc
     7141 aaattatagg tgatcgtgac atggacgaat tatctcaaca agagagtcga atggttcgga
     7201 agataagtaa gctaattatt tatcgggata cagaaagatt tctcactgtt ttaggtaacg
     7261 tctacgagaa tccagagtta ttggaggtag agtaattgcc aaaaatatta gatgcatgtt
     7321 gcggtagtag attgttctgg tttgataaga acaattctga cgtaacattt atggattgtc
     7381 gtcagcagta tgaagaatta tcaacaggtc atgtgatcaa tgttgatcca gatgtggtcg
     7441 cagactttcg cgatatgcct tttgaagatg atgtgttcga tatggttgta tttgacccgc
     7501 cacacttaag acatgtcggc gaaaactcat ggcttgccaa aaaatatgga aaattagacg
     7561 aactttggcc agaagacatc aggcaaggtt ttgcagagtg tatgcgagtt ttacgacctt
     7621 caggaacatt aatttttaag tggaacgagg aacagatacc actttctgat gtgttagagg
     7681 ctattggaga acagccattg tttggtaata aacgaagcaa aacacattgg cttgtgttta
     7741 tgaaaaaatt cagctaacgg aggaaacaaa ggaggaaaag taatgaagat tgtattaaat
     7801 aaatgttttg gtggctttgg gttgagccat gaagcaaaaa tggaaatttt taaaagaaaa
     7861 aatattgaag tattcccata catcaataac tttagctatg actcagatga tgaatacacg
     7921 agatacacag gccaaaagct aggttcgatg gattttatct actatttcaa aaaagacccg
     7981 aaaatcgaca aagttacagg aatttatagc gaaattgacc gactgtacgg aattgctgat
     8041 gattccagtt tttcaagtga ttcaaacaga ggtgataagg acctcgttgc tgttgtcgaa
     8101 aaactaggaa gtgaggccag tggaccatat gcaagtctta aagtcgttga tatacccgat
     8161 ggggctgagt gggaaataag tgattatgac ggcgttgaaa ctgcacatta tggttttcaa
     8221 acaggtagta tttagtccac taatgacaac taaagaggag gcagaaggat gagtgaagat
     8281 gaatactttg aacagtagtc tattaacgac agagattttt aactggaaag gggtgtagtc
     8341 atggacgcat cggaagtagt aaataaaatt aatagtctca aagaggtttt cggagacgtc
     8401 gaagtagtga taaacgcaca aggctatgac aacggatttt ttaagcagat taccgacatt
     8461 agcattcagc aaggaatgga agacgaagat ggaaacttta tcgatgaagt agccatttta
     8521 gtgacttgcg agtaagtaat tgacagctaa agaggaggca gaaggatgaa aaaattgcac
     8581 caactcatat ctgagaaaga aagcgaactc caaaatttag aagatagttt gggattgggg
     8641 tttccaattg ttgagcaagc taaaatgact caaatcagtc atctacgact tgaattggaa
     8701 gatttaagac aaatagaaaa atcaattcaa ctcaacgaca accagaaaat cgtgttggag
     8761 tggttgaaaa gcgagacaat tttaactaga gaagcaccaa tattatctgt taatgctttt
     8821 tctgataaaa atttactagg aaaattacct gataaagtac gcaaagctta taaactattg
     8881 gcttgtaaac aagaatatga agtcctatca gcattcgctc aatggggatt agaacaggag
     8941 gaagcggaat gaacaacaga catcgcagaa taacaaaact aagaaaacag gaactgaatg
     9001 tactaaagac aaagtttgaa aaagaatatg gaatttcagc agaagaaaca tataaagtgg
     9061 caagtcagtt tgttgctgat gcgagtaatg ctattcgtaa gtttgggatt tcgatattaa
     9121 atgatgatcg taaatgggag gaaatgagat gaaaataaaa gacggatttt acgctagtag
     9181 tcatggtatc ggcggtttaa tgctagacat gccgataaag aaccctaaaa cacgtcagaa
     9241 atcaaaattc aaaatcggtg acatggtccg atgtaaagca gaagagttca tctatccgtt
     9301 tcgtggatat gtagagcatg tctataatca ttcagcaatc attcacattg aaaacacgat
     9361 ggaatgcgat aagtggttag cgaaaagcaa agataattta gcagtggctc gtttggtgga
     9421 tatggaacta atcaatgaca aataaaaaag ccggatcgct ccgactgatt caataaatcc
     9481 aacacattta ttatatcaca taaaggagcg gtttgacttg atgcaattgt tacgagaggt
     9541 agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc ggcgtttgga
     9601 gcggatggca ggtcgctctt tgatagatat taagtcgccg attattacgg atatgccgaa
     9661 ggcaccgaag cacggcaata aggcagaaga cgcgatcatt cagatgatgg atatagaagc
     9721 ggagagagac gcaatactag cggctttgat ggctcttagt ctgattagcc gtcagatact
     9781 ctactacagc ttctgtgacg taaacaagca ctctaattat gaaatagggc aattgatacg
     9841 aggatacgga gagaagaatg tagagaagtt gaaatccatc gcattaatcg aatttgcaga
     9901 agcatacaaa aaaggcgtgt tagttcagta tcgttgattt tgtagggttt ttgtagggat
     9961 agtgtagggt ttttgagcgg tttaacgtga tattatggta gtgtcgaaag attagtgata
    10021 ggtctgagac aaaataaaat gtaagggagg aaatctccct catcgtttta acttaagctt
    10081 cgatagacag caacggaaat attaagaata aggatgtgaa tttcaactcc ttctaaattg
    10141 ttcttattat ctatcatccg ttgttgtcta ttaatttatg catcggagga aaaacgaatg
    10201 gataaagaaa tcaaagtaac tgtcaaatta gatttgactg aactaaaaga actgctcaac
    10261 aaggctagtg accaagtcga acagttacaa gaaactttag atgaaattgc taattttaaa
    10321 atccaagttt cttagcagta tattttttag cagctgcact cataaaatca taccaagtat
    10381 cagaaaaata taacttaact aaataattaa aaaaggtggg taaatagaaa tgacggcgtt
    10441 aataatttca atttttgcgc tctgtcttaa tgtctatatg attggattta aaaatggaca
    10501 aaataaaaaa tagtagcagc cgagaataat tttatagtgt cactgtggcg gaaatagaag
    10561 acgcaaaggt ggaggtagga agcgtatggc gcactatcgg gaccttgtac aaaactagaa
    10621 aggactagta cgtgtgtggt gcgagcccta gggaggaaca ggataaccgc ctgtgtgtag
    10681 gttgctatta catattagat cactctttga gtggtctttt tatttacata aaggaggcta
    10741 cataatgaga aactactggt atatatcgct aactaatgaa tatcctcgaa ccattgatga
    10801 ttgttcagtg cgtgttgtgc gttctgtcca aatcaaaaag aagtactcca ttgttgaaat
    10861 gaccagagaa gctacgccaa aagagattga taaatgcaaa cttctttatt gcggtcatgg
    10921 tttctatgat gaaccaaaca ttcaaaataa tattaacaag aatttgaggg attagaatgc
    10981 aagaaatggc ttaaaaatct ccaatcagga caaatcaagt tagacaaagt ttccgattta
    11041 aagatattga ttgaagctga tttaatgcta aaagatattg aaaattagaa aacaaaactc
    11101 aacctaagaa gattgcgagg tggtgtgtat tgaatggcaa gacaacgtga tccaagacgt
    11161 gatgaagcca aaagaatttg gttagaatcc aacggagaaa agcagttaaa ggaaattgca
    11221 tctgaattaa atgtttcaga ttctcaggtt agaaaatgga aatcgcaaga caaatggagc
    11281 gctgaattga aaagtaacgt taccaatggc aaaagtaacg ttactaatca aggtggcgct
    11341 cctattggta atcaaaatgc taaaggtaac aaaggaaaca gcagagcatc accgccaaag
    11401 agaaataaga atgctcttaa aactggtgag tatgaaacaa tattttttga cacgttaacc
    11461 gataatgaga aggacatcta ttctagtttg aatgatgatc cttcttttgc tttgtccgaa
    11521 gaaatacgac ttcttaaaat aagacaactt cgaatgatga aaagaatcca acaagctgaa
    11581 gctggactaa atgatgaaga agtcgaacga ttgcagcagc taagaaagat taaaactccg
    11641 attgaaaaaa atggtaaaaa gctagaaatc aagcgtgagg ttatgcaaga tgtgcagatt
    11701 agcagaaaaa aacatcgcaa aattgatgat attctttcaa ttgaagattc attgactcgg
    11761 attagcaacc agttagctaa agccatcaag caaatgaatg aactttatat gaatgaatac
    11821 agaactgatt taattaaagc tcagactgat aagatccaag ctgagacaaa tgaaattggc
    11881 ggaaataatt caggtgaaga aatagaagaa tggaaacagg cagttttaaa tgccgcaaac
    11941 aaacgggcgg tgaaagaaaa tgaataatga atttattcct tttgctgata ttggttctgc
    12001 cattgattat tactatgata aaccagtagc tttttgccaa gatattttgc atttgaatcc
    12061 tgatgaatgg caagaaaatg ttttaaatga tttagctgaa ttttcaaagg tttctgttcg
    12121 ttctggtcaa ggagttggaa aaacagcatt agaagcagga gcaatacttt ggttcttaac
    12181 gtgtcgaccc tacgctaaag taatagcaac agctccgaca atgaagcaac tttacgatgt
    12241 actttgggca gaggtagcta aatggttaaa tgatagcttg atcaaaaact tactgaagtg
    12301 gacaaagacc aaaatttata tggttggtga ttcagagcgt tggtttgcta cggctagaac
    12361 agcgactaaa ccagaaaata tgcaaggttt tcacgaggac catatgttga ttgtggtaga
    12421 tgaagcttct ggtgtgtctg atccaattat ggaagctatt cttggtacgc tatcaggttt
    12481 tgataataag ctgttgatgt gtggaaaccc taataatatt gaaggtgtct tttacgattc
    12541 ccacaattca gaccgtgata aatacagagt tcataaagta tcaagctatg atagtaaacg
    12601 tacaaacaaa gacaatatag aaatgattct taaaaaatat ggaaaagaaa gtgatgttgc
    12661 tcgtgtccgt atttttggag aatttcccaa aggtgcgttg gattcattta tcagtcttga
    12721 aacggttgaa ttggctacag aaaaacaaat tagtgattct ttagtcaata aaacaacggt
    12781 tgctcatatt ggtgttgacg tagctcgata tggtgatgat tctacgattc tctttcctag
    12841 aattgctacc agggcattgg agtatgagaa gtattcaaaa cgtagcacta tggaaacaac
    12901 aggatatgtc atcaacatgg ccaagaatct aatgagtcaa tatccgagta ttgataaagt
    12961 gatgattaaa gtcgatgaca ctggtgtcgg aggtggtgta accgaccgcc tagaagaact
    13021 tatagaagac aagcattatc cttttgaggt gtttggagtg aataacggtt caacatcaga
    13081 agacgatttt tacgataatt taggtactca actatgggga aacatcaagg aaatgttaga
    13141 agaaaatatg acagcaaatc ttaacggaga acagcctgtt attgaattgc cttctgatag
    13201 ttcgttaatc aaagaattaa gtactcgcaa attcaaaatg acaagtagaa gtcgtatacg
    13261 tttagaaagt aaagatgata tgaaaaagcg aaatattggt agtcccgata ttgctgacgc
    13321 actggcttta gcgttttatg agccaccaag tcactatcaa tttattcaat tttaggaggt
    13381 gagtttttga ttagtacagt attatcttta gagagatata agaagctacg tgttaaatat
    13441 gcaacacaaa ttgaagatgg aatgtttgat ccgaatggtt ttatagaata tatgaaacca
    13501 ttttttgctg atcgcgaaag aaaatatcta gcttatacta gcgaaaagaa tgaaatagac
    13561 aatagaccaa agcctaacac tgatattgta aaagttaata ataaacttca tgctggtatg
    13621 tattcaattg tcgtagacca agcagtcaat cattttactg gtatacctat caaatgggat
    13681 tacgatgtat cggaacaaaa aagaacactc attcaaagat taaaagataa attcttaaaa
    13741 aatgatatag aacttccaac agttccagaa gcttttaaca aattaacaag caatcttgat
    13801 tccatgagat tcgcaatgct tgattctgaa actgcaactt ttcaaggagc ttgtggagta
    13861 gctttccgat tgttagagcc tgtggaagaa gatgatggtt ggaagttaag agcaagtaat
    13921 attgaacctt ggagagcaga aagatatgga aatgccggaa tctatatcaa agaaaagtat
    13981 gactcttacc agaaaaaatt ttttcaagaa atgaaagtta tgacaagaaa taaaattctg
    14041 acatatgctt gctatggtga tttgaatttt gttacaagcg gaacgttcaa aaaaattgat
    14101 gagaccgata accctttggg gacgattacc ttggcagaat ttaaaaataa tacgaatcgt
    14161 tattgtgatt ttgaagtagc tgaggaaatt ggagatgcaa ttgatcgtgc tttgtctgat
    14221 caacaaaacg aaatcgaaca atttaagctt gcgtacatgc ttgttactgg cacaacaatg
    14281 agcaaaggca ctgctaaaga aatgatgaat cagctaggga ttatcaactt aaaagaccct
    14341 actgcaaaag ctgaatatgt cacaaaaaaa ttagcaaaag atttcaatga atatcatatg
    14401 gatctattga aaaaacagtt ttacaccatt tgtaaagcaa tcgattttaa cgatgaggtg
    14461 tttaaatcaa acagttctgg agaagctcgc aagtggcaaa tcattagcct agaagcgaaa
    14521 acaaatacta aagagcaata ttttagagaa ggattgaaag aatgcgcaga aacaattgct
    14581 gcttttctta agtttcatga caaagtagaa attgagcctg aaaaaattat ttttactttc
    14641 tctagatctt tgccaactga tttaagttat ttagctgaag ctttacctaa acttgcacca
    14701 tacgtatcca aacgaactat tcagagccaa attccatttg taacagatgt tgattatgaa
    14761 aatgaaatga tggaattgga aagtggaagt gcttatccag atagtgaata caattttggc
    14821 ggaggtggca atagtgacga tagaaacgaa gtattggacc aaacgtcgag aactggagga
    14881 tcaagcaagg ctcaaacaag aaaatcagac acttaaaaaa ttaactagtg tatttcctga
    14941 agcactgaaa gagatacaag caaaactatt atcacaagct gacttacaca atatcactta
    15001 tccagaaatg atggagtttt acagtacaag taatcagaaa aaatatcgtg aatatgtgga
    15061 aaaaaattat aagtcattaa aaatgtatga tgcaaaatac aaagagttta tcgatgaatt
    15121 ttttccacca tttgactatg caaaagtcaa tcgcttatta caaatacgat cagatgtatt
    15181 taaaattctt gcagaatatg cgatggatgc agatgtgaat caatattttt ctgatcgctt
    15241 agaggaaatt cttcaaagaa catattcttc taatgctaat gtttttgttc aacttttaaa
    15301 cgttgatata ccaaattatt taccagaaaa tgaaattaaa cattatttga actatccatg
    15361 gtcaggaaag acattttcaa gaagactttg gggaaatatt tcatcgcttg aacagaaact
    15421 ctctaatgct attgtaaaaa gtgttgctag tggagaaggc gttatacacg cattaaacac
    15481 catgagatta gactcagaaa tttgtgacat gtttaagtta gaagaatcaa agtataacaa
    15541 agcgatagaa aatctcgttc gaacggagta tgcaaaattt gcacaagatg gtattgaaaa
    15601 atcatattta gaaacaggta ttgaggaata caacgtattg actgcaaaag atgagagagt
    15661 ttgccggatt tgtggaggaa aggcaagtaa gaatccctat aaactgaaag atgctgtcat
    15721 aggtgaaaat cgagcacctt tccatagtcg ttgtagatgt acggatgttc ctaatttacc
    15781 aaaattagga aaggatattg atgaagaata tgaccgttta tttggcgatt tattagatga
    15841 gtttgcacat gattcttttg gaattaattt gaaacggagg aagtagaatg aaagattttt
    15901 ttgaagcagt actaacaatt aatgtaaatg ctgatattgc agaagcctac aaaacagcta
    15961 ttgagtctga gaaccatcct aatggcttga gagaccattg gaatggcaat tatgcctacg
    16021 tggttattgg cgatcaaact gttaattatc aagataatac tccagttgat aagaataccg
    16081 ttaatttaac gattcaatta ttatctcatt cattaccaaa tttaaaagaa acagttgatt
    16141 ggtatgaaaa gatgggatgt attgttgtta gaactgacta caaagaagga aagtctagta
    16201 attaggcttt tttattttgt ccgaaatgac gttaaactag cgcaatactg ggcttggttg
    16261 aatggtgggg cgcaactatt aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg
    16321 gcgaaaggag aatgagcatg aaacacaaat cattaatgcc aatgaatttg caatactttg
    16381 ccgaaggtga cgatcaaaag ttttcttttg atgacttcaa atcttttgtg gaatccaatg
    16441 aagaagctca aaaatttatt cagtcacaat ctcaaagtgt tgccgacaaa caattggaag
    16501 cttggaaaca aaataactta gataaaatta aacaggatac catcaaggaa tatgaggaat
    16561 ctaagaaaaa caagtcacct gagcaaattc aactggaaaa actacaagct gaatttgaag
    16621 cagaaaaagc gttgcgtgtg acaagtgata ataaagcatt tgttgcagaa caaattgctg
    16681 gattagaact agatggagag ttaaaagagt ctatttctca atttatgcta aataatcttg
    16741 ttagttcgga tacagatttc actaagaatg ctgttgaagg ttttacaagt gttttgaatg
    16801 caattaatga gaaacatgca gatgcattaa aagaactaca aatgaagtct gcatttggtg
    16861 gaactcaaca atcgaataac caggttcagc agaacaatga aacattaaca aatccagaag
    16921 aacaattagg acaaattctt caacaattta actaggagag tgaaaaatta tgaaaaaaac
    16981 atctttaaat aatttagagt atttggatat ttcaccagcg attaatgcta tgcaagtacc
    17041 aaatacacct tttttaagct atttacttgg tgctggaaaa acagagccag caaactcgac
    17101 agaaattaaa tggcgtgaat atgatatcaa caacgatgat tcttctgaaa aacttgaggg
    17161 cggagaatat ccagatgctg aatcaggtcg aacttggttt aacaactata ctgaaatttt
    17221 tagaaaatca acctctgtat ctggtacatt agatgctatt aatgtgaatg gagtcggaaa
    17281 tgaattaact aatcaagtag cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa
    17341 attgattact ggtgtaaaag ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat
    17401 tttgaacttg atcaattcag caaataaggc agaaacagcc actgcgggtg cagtaacaag
    17461 aaaagatatc gatactttat ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact
    17521 atgcttgatt tctccagata tgcaggagtt aatgactgat gagttagatg gaaaatcaac
    17581 aaaaattgtt cagttcgggg aaagagtaac ttttggattg caaattggaa atatcgtgtc
    17641 taattacggt acaggtattg ctctactaga accatcattg ccaaaaggaa caattgccgc
    17701 aatcgatact aattatgtga aactacgtcc attacgtgaa tggagagcag aagaacttgc
    17761 aaaaacaacg gattctagac gtatcggtct tgtaggtgaa tactctcttg aatacaatgc
    17821 ttcaaactct ggggcaattt taaatttaaa gtctgaataa aagggagtta gtactccctt
    17881 ttttgatagg aggaattaca gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga
    17941 aatatagagt aggtaaaact aaaaattttg ttgggtttgt tcatcctaaa actcgtagat
    18001 ttatcacagc agattcaaat aacgaattta tcatttctat agatgataaa gaagcaattg
    18061 caattttgga agatgcaatt gatgttaatg aaatttagga agtgatctga tggatgaatc
    18121 gctaaaaacg gaaatcattg agtctacaaa agaagatttt ccagatttga gtgaagaacg
    18181 cataactaat ttattagaaa taattttgct agaaattgaa tcatacaaca cttgtaaaaa
    18241 tgatatttca tgggaaaagt taaaaagcgt gattagtgaa gtgttgtata aaataataaa
    18301 aaatgaatca gaaaaaacag tatcttcagt tagacgtggc gatacaacga ttagttatgc
    18361 ttcaacaaca aatgatgtta gtgagttact tctaggctat ggtgatttga tacgaagagt
    18421 tattggttgt ggaggattgg agttttttta atgaatgaag cagatatttt agaaactact
    18481 tatgaagata gttgtattat tgaaagactg acggacattg aagattctaa tacaaatatt
    18541 actattcaag attacaaaaa agtatatgat aatcctattt cttgcgctct ttcacaaggt
    18601 caaatcgatg gactagcagt catagaagat ggagagatgg taaatgtttc aactgacaca
    18661 tataaattat ttgttcatcc taagattaaa ctaaaaaaag gagatcgaat aacaataact
    18721 caaaaggcca gtggcttaat tttttctcta tttgctacta agccttttta ctatcctagt
    18781 cattgtgaag tgaatttgat aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa
    18841 tgcagaaaag atcattgaga attttaaaaa tatgactgta attgctcaaa aagaaggaat
    18901 atccttcgtt aacgattcaa tgaataaagt cgttagttta gctaaaccat taactcctgt
    18961 aaaatcaggt aatttaaggc gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg
    19021 tcgaattgtt ggagcagtta taaacaatga acattatttt aaatatgtag aagaaggtca
    19081 taggactaaa aatggtggct ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct
    19141 tgcaaatatg tcttatattc ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa
    19201 aggaaagtaa catgaaagat gaaatcattg ctgcaatcag tagcaaatta aaagaaatct
    19261 atcctgatgg gacaatatat cttgattcag ttatgcagtc aactaaagat ttttatttcg
    19321 ttttatcagt aatggaatct ggaactgaaa atgtaggaat tgatattcaa aatgtttctt
    19381 tcttaattg
//
LOCUS       DAZAJZ010000053        18899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000053 DAZAJZ010000000
VERSION     DAZAJZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.053"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1125)
                     /gene="recA"
                     /locus_tag="ACG86D_001790"
     CDS             complement(76..1125)
                     /gene="recA"
                     /locus_tag="ACG86D_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN3140928.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1234..2475)
                     /locus_tag="ACG86D_001791"
     CDS             complement(1234..2475)
                     /locus_tag="ACG86D_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN3140929.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2715..3293)
                     /gene="pgsA"
                     /locus_tag="ACG86D_001792"
     CDS             complement(2715..3293)
                     /gene="pgsA"
                     /locus_tag="ACG86D_001792"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN3140930.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3502..4425)
                     /locus_tag="ACG86D_001793"
     CDS             complement(3502..4425)
                     /locus_tag="ACG86D_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN3140931.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5164..6450)
                     /locus_tag="ACG86D_001794"
     CDS             complement(5164..6450)
                     /locus_tag="ACG86D_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN3140932.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6443..7711)
                     /locus_tag="ACG86D_001795"
     CDS             complement(6443..7711)
                     /locus_tag="ACG86D_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN3140933.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7963..8424
                     /gene="mscL"
                     /locus_tag="ACG86D_001796"
     CDS             7963..8424
                     /gene="mscL"
                     /locus_tag="ACG86D_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN3140934.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8483..8929)
                     /locus_tag="ACG86D_001797"
     CDS             complement(8483..8929)
                     /locus_tag="ACG86D_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3140935.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9022..9267)
                     /locus_tag="ACG86D_001798"
     CDS             complement(9022..9267)
                     /locus_tag="ACG86D_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN3140936.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9382..9960)
                     /locus_tag="ACG86D_001799"
     CDS             complement(9382..9960)
                     /locus_tag="ACG86D_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3140937.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10037..10567)
                     /locus_tag="ACG86D_001800"
     CDS             complement(10037..10567)
                     /locus_tag="ACG86D_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN3140938.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10571..11431)
                     /gene="rlmB"
                     /locus_tag="ACG86D_001801"
     CDS             complement(10571..11431)
                     /gene="rlmB"
                     /locus_tag="ACG86D_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN3140939.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11424..11819)
                     /locus_tag="ACG86D_001802"
     CDS             complement(11424..11819)
                     /locus_tag="ACG86D_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN3140940.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11816..13228)
                     /gene="cysS"
                     /locus_tag="ACG86D_001803"
     CDS             complement(11816..13228)
                     /gene="cysS"
                     /locus_tag="ACG86D_001803"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN3140941.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13225..13761)
                     /gene="epsC"
                     /locus_tag="ACG86D_001804"
     CDS             complement(13225..13761)
                     /gene="epsC"
                     /locus_tag="ACG86D_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN3140942.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13847..14086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14083..15543)
                     /gene="gltX"
                     /locus_tag="ACG86D_001805"
     CDS             complement(14083..15543)
                     /gene="gltX"
                     /locus_tag="ACG86D_001805"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN3140943.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15713..16870)
                     /locus_tag="ACG86D_001806"
     CDS             complement(15713..16870)
                     /locus_tag="ACG86D_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN3140944.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16904..18274)
                     /gene="radA"
                     /locus_tag="ACG86D_001807"
     CDS             complement(16904..18274)
                     /gene="radA"
                     /locus_tag="ACG86D_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN3140945.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18337..18864)
                     /locus_tag="ACG86D_001808"
     CDS             complement(18337..18864)
                     /locus_tag="ACG86D_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN3140946.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4995 a         4173 c         3389 g         6342 t
ORIGIN      
        1 tttattcaat aaaaaaacgg caggtcaagg agagtaccta ctcttcagac cagccgtttt
       61 tgtttatcat aatttttatt cttctactaa aggcaattct tcttgtcctt tttcatcttc
      121 tgggacagcg acttcttcac caataccata agctgctcta actagagcag aaacttctgc
      181 catcatttca ggatgattag ccatatagat tttagcgttt tcccgtcctt gaccaatccg
      241 atcttcttta taagaatacc aagcaccgct tttatcaaca atgtcttttt ctactgccat
      301 gtctagaagt tctccttctt gtgagatccc taaaccatac atgacatcta cttcagcgat
      361 tttgaaagga ggtgctactt tgttttttac gactttgatc tttgtacggt taccaacaat
      421 atctgtacct tgtttcaatt gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa
      481 tttcaatgcg cgtcctccag gagtgatttc tggattccca aacatcacgc ctactttttc
      541 acggatttgg ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac cagagagttt
      601 acgcaatgct tgagacatca aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc
      661 gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg actactacga tatctacagc
      721 cccacttgat actaaagcat cagcgatctc tagaccttgt tctcctgtgt caggctgtga
      781 aagaagtagt tcatcgatat tcacacctaa tttttgtgca tattgcggat ctaacgcatg
      841 ctcagcatca atgaaagcgg ccgttccgcc atttttttgt acttctgcaa tagcgtgtag
      901 tgcaactgtt gttttacctg aactttcagg tccatatact tcgatgatac gtccacgggg
      961 gtaaccgccg acacctaaag cgacatctaa cgcaagagag ccacttggaa ttgtagagat
     1021 ttgttggtcg attttttcgc ccaatttcat aatagagcct ttaccatagc tcttttcaat
     1081 ttttttcagt gcagcatcta aggctgcttt acgatcatct gccaatacga taatcctcct
     1141 tatatttaca aatcagagca acatttcatt atccttatca tatctttttt agtgaaaaaa
     1201 agcaagaaaa aaccgaacaa atattcgcat ttatcatttt cttaaaagct gttttctcaa
     1261 aagatcaaat ccttgcataa ccgcattata tctgatagca gcacgatctc tggcaaagtg
     1321 atatttttcc gaaaccacgc cgtctttaga tgaaatagcg atccatactg ttcctgcaga
     1381 ttgaccttct aacgtgtccg gtccagccac tccggtgaaa gctacactgt aatctgttct
     1441 tgctatctca cgtgcttgaa tggccatctg ctccacacat tctttgctga ctgttccata
     1501 ttctgctaat agctccggat caatctccag aaaaccagct tttgtttgga gcgagtaggt
     1561 aacgaatccg cctggaaata cttcagatac accagcaatg tcgcctaatg cggcttggaa
     1621 ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt tcagtagttc
     1681 cactacgact tttgctagcg aattatcatc gccgtatcca taaaaatatt cccctactcg
     1741 ttcttggatt ttttcttcta atgctaaaag agcttgattc cctgcctgaa catcatttgt
     1801 tttcactgtc agtcgtaatg tcacttcatt tggtttggca tatggtgcga tcgttggatt
     1861 gatctgtgtc tcaatcagat cttttagctc agtgaccagt cgcgattcac caatcccata
     1921 aaagcgcaaa actttagaaa ttagtttttc ttcagaagga aaacgttcct ctagtaaagg
     1981 acgtacttgt tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa gaaggatata
     2041 cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc cctgtacgat ttggtagagg
     2101 aaccccgcct tcgataatct gggactgttg cagattgttt ttggtcattt ttcgatgggt
     2161 tgtttcaaaa tatgccaata attttttgta tccttccgta ttttgtatga gagacttacc
     2221 gaggtgagcc gcagttgctt cttttgtcag gtcatcttct gtcggtccta atccgccaca
     2281 taagagtatg agttcacttc ttgtttcagc tagagcaatc aactcttcta aacgctgctg
     2341 attatctcct acaactgttt gataataaac ttcgatacct agatcagcta gctgttctga
     2401 caagaaagtg gcattcgtat tcacgacttg tcctagtaaa agttctgttc cgacagctat
     2461 tatttctgct ttcattactt cccgctccct tatatagtat acgctgtatt tttatatttt
     2521 tttatttaaa aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa
     2581 ataaacagtg ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa
     2641 aagctgtttt ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct
     2701 ccactaaacg atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc
     2761 acacctgaat agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata
     2821 ccgatcaggc tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta
     2881 gccgtcttga cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt
     2941 aaacgcaagc ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg
     3001 gcctttcctt ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac
     3061 gggtccgcaa acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct
     3121 aaccagtctg tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc
     3181 agtgtggtgt ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg
     3241 aaaatcggga tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg
     3301 actcctttct tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt
     3361 ttccaaatga atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga
     3421 agaaggaccg cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga
     3481 tgcgtaaact aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg
     3541 actctgattg ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc
     3601 attgcttgct gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact
     3661 ctgtgtttca tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc
     3721 taaccataca cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat
     3781 atttactgcg atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc
     3841 acttgatttt gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga
     3901 ggaagaggac tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat
     3961 tggatcagct tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc
     4021 aaccaatcct aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc
     4081 ctgatgcatt gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga
     4141 tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac
     4201 agcttcagcg tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa
     4261 atcatcattg tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc
     4321 atctaaagat atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa
     4381 agccactcgt tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga
     4441 aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa
     4501 acattgttct cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc
     4561 tgttttttga acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct
     4621 taaacggtgt tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga
     4681 gatatttttc cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc
     4741 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt
     4801 aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     4861 tcttggtaca cggtgttcca gaagtagctt cttcggcaat aagccgaaat atcacaaaaa
     4921 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca
     4981 acctctttga ttacttcgtt tgattttatt ttaatataag ttgattaaaa gagagcatga
     5041 tttgttttat tttctcacta atttttccaa cacaactaag aataccataa aagctgattt
     5101 tggaggaggt tcctactaca agtctaaaac gaaatttaag acttttcctc ataaaatact
     5161 ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa atgcttcccc
     5221 agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt
     5281 tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga
     5341 ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc
     5401 cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc
     5461 gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc
     5521 aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc
     5581 taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc
     5641 ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc
     5701 ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc
     5761 ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc
     5821 aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata
     5881 gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc
     5941 gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt
     6001 agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt
     6061 ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac
     6121 ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga
     6181 agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg
     6241 ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat
     6301 aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc
     6361 atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc
     6421 attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg
     6481 cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac
     6541 gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag
     6601 cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt
     6661 ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt
     6721 gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga
     6781 atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct ttctctctga
     6841 cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg
     6901 caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt
     6961 gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa
     7021 acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg
     7081 ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt
     7141 cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca
     7201 accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag
     7261 caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca
     7321 agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt
     7381 ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa
     7441 aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc
     7501 ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgaa taattcaaac
     7561 tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac
     7621 gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa
     7681 gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg
     7741 cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt
     7801 ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag
     7861 atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt
     7921 tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa
     7981 gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg
     8041 tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt
     8101 gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg
     8161 ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg
     8221 atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc
     8281 tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac
     8341 atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca
     8401 aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg
     8461 tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg
     8521 gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag
     8581 cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca
     8641 tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa
     8701 caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga
     8761 tcttcccatc agtatcttga aaaaacgtat gccaagcttc tccatcaatg tcatcaactt
     8821 cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt
     8881 aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc
     8941 cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg
     9001 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
     9061 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
     9121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
     9181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
     9241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
     9301 tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa
     9361 gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt
     9421 gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc
     9481 catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga
     9541 aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa ttgctaaagg
     9601 ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat
     9661 tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata
     9721 gatttgattg cttataacgc ttttataata acttccaaag ctaacacctt tgcttttttc
     9781 gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac
     9841 ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata
     9901 cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat
     9961 aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac
    10021 tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt
    10081 tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt
    10141 tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag
    10201 aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca
    10261 ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag
    10321 ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga
    10381 tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat
    10441 aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa
    10501 gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt
    10561 ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag
    10621 caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca
    10681 tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg
    10741 atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa
    10801 atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga
    10861 catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt
    10921 gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa
    10981 aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt
    11041 ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga
    11101 ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga
    11161 ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat
    11221 cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt
    11281 gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc
    11341 gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg
    11401 attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag
    11461 cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct
    11521 tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt
    11581 ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc
    11641 agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga
    11701 tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg
    11761 taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg
    11821 cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca
    11881 acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt
    11941 cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga
    12001 agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt
    12061 tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat
    12121 ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca
    12181 aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca
    12241 aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt
    12301 cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg
    12361 gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt
    12421 tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt
    12481 tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt
    12541 gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt
    12601 caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt
    12661 ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg
    12721 tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat
    12781 tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt
    12841 caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca
    12901 gtgtagcagg ttcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat
    12961 cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta
    13021 catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa
    13081 ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg
    13141 gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc
    13201 tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg
    13261 aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac
    13321 ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt
    13381 cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt
    13441 gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc
    13501 aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg
    13561 gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag
    13621 ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta
    13681 tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat
    13741 cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat
    13801 catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt
    13861 ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat
    13921 acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa
    13981 gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc
    14041 tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt
    14101 taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc
    14161 atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc
    14221 agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat
    14281 ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac
    14341 ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac
    14401 gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc
    14461 gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca
    14521 catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag
    14581 ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat
    14641 caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat
    14701 aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa
    14761 gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat
    14821 atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt
    14881 attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac
    14941 cgcaacagca aaattataag ttggcatacc atcgcgtttt tggatgacaa agtcgccacc
    15001 gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt
    15061 attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg
    15121 ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg
    15181 ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt
    15241 gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta
    15301 agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag
    15361 gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat
    15421 acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct
    15481 tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt
    15541 cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac
    15601 ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta
    15661 aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg
    15721 attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag
    15781 gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga
    15841 tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt
    15901 aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac
    15961 cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat
    16021 tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa
    16081 gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt
    16141 acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac
    16201 gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa
    16261 caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc
    16321 gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt
    16381 ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc
    16441 gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat
    16501 agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat
    16561 tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac
    16621 cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag
    16681 ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc
    16741 tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg
    16801 agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac
    16861 gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct
    16921 tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt
    16981 tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga
    17041 ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga
    17101 aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag
    17161 ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt
    17221 gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat
    17281 tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg
    17341 cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag
    17401 ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc
    17461 catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg
    17521 ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca
    17581 tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa
    17641 aaatggctat cccatttgtt ttagcggttt tgagtagttc tgcagtggtt tctcttactt
    17701 ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa
    17761 tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt
    17821 cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct
    17881 gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct
    17941 gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca
    18001 tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga
    18061 tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg
    18121 tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat
    18181 tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt
    18241 ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca
    18301 tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc
    18361 gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc
    18421 ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg
    18481 gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac
    18541 tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa
    18601 aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg
    18661 tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt
    18721 ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc
    18781 agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat
    18841 aacttcaaat cctctttttc tcaccggttt tcctccctaa ataaactact cctattcta
//
LOCUS       DAZAJZ010000054        18504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000054 DAZAJZ010000000
VERSION     DAZAJZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.054"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG86D_001809"
     CDS             99..746
                     /locus_tag="ACG86D_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN3140947.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG86D_001810"
     CDS             1139..3784
                     /locus_tag="ACG86D_001810"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN3140948.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG86D_001811"
     CDS             4094..5407
                     /locus_tag="ACG86D_001811"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN3140949.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTEAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG86D_001812"
     CDS             5397..6050
                     /locus_tag="ACG86D_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3140950.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG86D_001813"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG86D_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFN3140951.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG86D_001814"
     CDS             6947..7147
                     /locus_tag="ACG86D_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3140952.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG86D_001815"
     CDS             complement(7401..9080)
                     /locus_tag="ACG86D_001815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN3140953.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG86D_001816"
     CDS             complement(9082..9294)
                     /locus_tag="ACG86D_001816"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN3140954.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG86D_001817"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG86D_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN3140955.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG86D_001818"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG86D_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN3140956.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG86D_001819"
     CDS             13269..13466
                     /locus_tag="ACG86D_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140957.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG86D_001820"
     CDS             13619..14104
                     /locus_tag="ACG86D_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN3140958.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG86D_001821"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG86D_001821"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN3140959.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG86D_001822"
     CDS             14981..15508
                     /locus_tag="ACG86D_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140960.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG86D_001823"
     CDS             15599..16336
                     /locus_tag="ACG86D_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN3140961.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG86D_001824"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG86D_001824"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN3140962.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG86D_001825"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG86D_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN3140963.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgaagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZAJZ010000055        18270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000055 DAZAJZ010000000
VERSION     DAZAJZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.055"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1132
                     /gene="bsh"
                     /locus_tag="ACG86D_001826"
     CDS             158..1132
                     /gene="bsh"
                     /locus_tag="ACG86D_001826"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN3140964.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            1457..3352
                     /gene="asnB"
                     /locus_tag="ACG86D_001827"
     CDS             1457..3352
                     /gene="asnB"
                     /locus_tag="ACG86D_001827"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN3140965.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3547..3993)
                     /locus_tag="ACG86D_001828"
     CDS             complement(3547..3993)
                     /locus_tag="ACG86D_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN3140966.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            4221..4370
                     /gene="rpmG"
                     /locus_tag="ACG86D_001829"
     CDS             4221..4370
                     /gene="rpmG"
                     /locus_tag="ACG86D_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3140967.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4458..4976
                     /locus_tag="ACG86D_001830"
     CDS             4458..4976
                     /locus_tag="ACG86D_001830"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN3140968.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5053..6009)
                     /locus_tag="ACG86D_001831"
     CDS             complement(5053..6009)
                     /locus_tag="ACG86D_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3140969.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            6175..6981
                     /locus_tag="ACG86D_001832"
     CDS             6175..6981
                     /locus_tag="ACG86D_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3140970.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            6978..7967
                     /locus_tag="ACG86D_001833"
     CDS             6978..7967
                     /locus_tag="ACG86D_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN3140971.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            7964..8968
                     /locus_tag="ACG86D_001834"
     CDS             7964..8968
                     /locus_tag="ACG86D_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN3140972.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            9100..9642
                     /locus_tag="ACG86D_001835"
     CDS             9100..9642
                     /locus_tag="ACG86D_001835"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN3140973.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            9662..10360
                     /locus_tag="ACG86D_001836"
     CDS             9662..10360
                     /locus_tag="ACG86D_001836"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN3140974.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            10381..10593
                     /locus_tag="ACG86D_001837"
     CDS             10381..10593
                     /locus_tag="ACG86D_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN3140975.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            10593..11555
                     /locus_tag="ACG86D_001838"
     CDS             10593..11555
                     /locus_tag="ACG86D_001838"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN3140976.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            11573..11968
                     /locus_tag="ACG86D_001839"
     CDS             11573..11968
                     /locus_tag="ACG86D_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3140977.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            12131..12496
                     /locus_tag="ACG86D_001840"
     CDS             12131..12496
                     /locus_tag="ACG86D_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN3140978.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            12493..14304
                     /locus_tag="ACG86D_001841"
     CDS             12493..14304
                     /locus_tag="ACG86D_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN3140979.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(14366..14533)
                     /locus_tag="ACG86D_001842"
     CDS             complement(14366..14533)
                     /locus_tag="ACG86D_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN3140980.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            14782..15771
                     /gene="galE"
                     /locus_tag="ACG86D_001843"
     CDS             14782..15771
                     /gene="galE"
                     /locus_tag="ACG86D_001843"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN3140981.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            15783..17267
                     /gene="galT"
                     /locus_tag="ACG86D_001844"
     CDS             15783..17267
                     /gene="galT"
                     /locus_tag="ACG86D_001844"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN3140982.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            17291..>18270
                     /locus_tag="ACG86D_001845"
     CDS             17291..>18270
                     /locus_tag="ACG86D_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3140983.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         5756 a         3126 c         3845 g         5543 t
ORIGIN      
        1 ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt ttttataaat
       61 gctagtaaat caatcttctt tggccttttt tttgataaaa aaaaatacta gatgtaagat
      121 aaaagtgtaa aaacgatttg ttttaggagg gaaaacgatg tgtaccgcaa tcacttatgc
      181 aacgaaagac cattattttg gaaggaattt tgattatgag atgtcttata atgaagtagt
      241 cactattact ccaagaaatt atcgtttcga ttttagaaag gtaaaaaatt tagataaaca
      301 ttatgcaatg ataggaatag cagctggggt atccaactat ccactctatt atgaggctac
      361 aaatgaaaaa gggttgagta tggcaggact gaattttcct ggcaatgctg attataaaga
      421 attacaagaa ggaaaagata acgttgcacc atttgagttt attccatgga tactagggca
      481 atgctcaacc atagacgaag ctaaagaatt attggctact atcaatctag tcaatatcga
      541 ttttagtgaa aaactaccct tgtcaccttt acactggtta ttagctgata aagaaaaatc
      601 gatagtaatt gaaagtatga aagatggact tcatttatat gataatcctg tcggtgtgtt
      661 gacaaataat ccgccatttg attatcaatt atttaatctg aacaattatc gctcgctatc
      721 aaatggaaca ccagaaaatc atttttcaaa ccagatcagc ttgaatgttt atagtcgtgg
      781 gatgggagga ttgggcttac ctggagatct ctcttctgtt tctcgatttg taaaagccac
      841 atttactaaa atgaatgctg catcaggtga ttcagaatct gaaagtatca gtcaattttt
      901 tcatatacta ggctcagtcg agcaacaaaa aggagtatgc gatactggag aaggaaaata
      961 cgaatatacg atttattctt cttgctgtaa cgttgataaa gggatttatt attatcgtac
     1021 gtatgaagac agtcaaatta ctgctgtaaa tataaataaa gaagacttgg atcgtcatga
     1081 attggtcagt tatccagtca taaaagagca acagataaac tatgtcaatt aattaatgaa
     1141 tagatccgtt tggtatcata ttttctgata cagtcataat tagacacgat ctttaggtcg
     1201 tgtctttttt tgctgttttt tttggaaata gcatccacta atattttttt aacacgaaga
     1261 gaataaggta gcctcaaaaa agaaaacatg gtaaattaga tattgggact ttattttctg
     1321 taaaaacaga aactttcatc ttttgttttc acgtgacgaa attgtaagag agaaaatatt
     1381 cgtccgctat ttattctatg ataagatgca tatgaatgaa ataaacagta attttatatt
     1441 aggatgtgaa agttaaatgt gcggtattgt aggttttgtt aacgacaagg acaacaaaaa
     1501 aacaattatt aatacaatga tggatcgaat cgttcaccgt ggacctaata gttcaggaga
     1561 atatatcgat aaacatgttg ctttaggatt cagaagatta agtattattg acttagaggg
     1621 cggtactcag cctatttaca atgaagatcg gacaaagatc attattttta atggcgaaat
     1681 ctataattac cagcctttga gagaagaatt gattgcagct ggccacgttt ttcaaacaca
     1741 tgcagataca gaagtccttt tgcatggtta cgaagaatgg ggaacagaat tattgcaaaa
     1801 gatccgcgga atgtttgctt ttgctatttg ggataatgaa aagaatgagt tattcggtgc
     1861 aagagaccac tttgggatca aaccatatta ttatgcagaa atgaacggaa catttatgtt
     1921 tggttcagaa atcaaaagtt ttctgcctca tcctgatttt aacaaagaat tgaacaaaga
     1981 agcgttgaaa ccttatatga ctttccaata ttctccatta aacggcgaaa cattcttcaa
     2041 agatgtctat cgtttgcctg aaggccatta ttatacgtat aaagatggaa aactagacat
     2101 tcagcaatat tgggatgcag attttgaaac aaaagaaacg cattcccgac aagaatggat
     2161 cgaaaaaatc gacgaaaccg ttcaagcttc tattgaagca catacggtca gtgatgtaga
     2221 agttggttct ttcttatcca gcggggttga ttccagttat gtaacctctg tattgaaacc
     2281 agatcattct ttctcaatcg ggtttgatga taaaacctat aatgaagcga ttgaagccag
     2341 aaaactaacc gaactattgg acttagataa tacagcagca gtgatcgacg gagacatgtc
     2401 gttcaaagca ttccctttga tccagtatca cttagatgaa cctgattcaa atccttcttg
     2461 cgtacctttg tactttttag caaatcttgc ttcacaaagt gtgcgggtag tccaatctgg
     2521 agaaggggcg gatgaactgt ttgcgggata ccaaacgtat ggattccata cgaattcaaa
     2581 attcatccgt gtgattgctc aaggattgaa aaaattacca aaaggaacac gctataattt
     2641 aggacgtaaa atcggaaaaa tgaaaaattt ccatggacgg atccatttat atgaatcact
     2701 tgctcctgca aaagaatatt ttatcggtca tgctcgagtg ttcgaagaat cagaagcatc
     2761 agaagtgtta acaccaaaat atcaaacagc accatctgtg gatgaaatca tgactgttca
     2821 ttacgagaaa acagaaggaa tcaaagatga agtcaataag atgcaatatg tcgatcttca
     2881 tcaatggatg ccaaaagata tcttgttaaa ggcagataaa ctttctatgg caagttcttt
     2941 ggaagtccga gtacctttat tggatatcga agtcatgaaa ttggctcaac aaataccaag
     3001 taagttttta ttgaatcaaa acaacacaaa agatattttc agacaagctg cgaacaaaca
     3061 tcttccggaa gaatggtcga atcgtgtgaa attaggattc cctgtgccaa tcaaagcttg
     3121 gttgaaagaa gaacacggct acgaacaagt caaagcatta ttcgaagcag attttgcaaa
     3181 agaatttttt gatcaagaaa aaatcatgaa gttgctagat gaccatcacg aaggacgtaa
     3241 agaagagcaa agaaaaatct ggacaatctt cagtttcttg acatggtata aagtctactt
     3301 tgtagatgaa acgattccgc aagcagaagc aatcgactat gtgactgttt gatgaaaatc
     3361 aaatatcaat aaaaaagagg ttttctccta ccgtttaaca ctgagtgata agaaacaatt
     3421 tcaaaaaaca gctcttcccg ttttatagat attatgactt atggttcgga gttggcagag
     3481 gaacaccatt gtttcgaaca taagggggtt gtgacacgat tttcgtcaca acccctttac
     3541 atatgttcaa taatcgattt ctttttcttt tgtcccatct tttttataaa tgaacaaagt
     3601 tgattctttg ttttgtgcta cttgctttcc tttttcgatt gcttcttttt ttgtatcaaa
     3661 atggttgctt gccttttttg cccctttaga tttcacgatc cattgttctt cctcatattc
     3721 tacgatcaca tctgaatcaa gcagttttcc agcacgagga ttcgtatcgt gttcatccgt
     3781 cttgcttgga tttttttctt tttcgaaagt tcgtttttct gattcactag catcttcata
     3841 ccattttttt gcttgatcaa tggcaattgg gatcgcacgg ttatcagggt acccttcatc
     3901 taataatgca tttgcgatgt cgattgcttt ttttcttgtc aatttatcta agtttttcat
     3961 tgatgcagga aagtccttca tgttccatgg catattagta tcccccttta ttaaaaaaca
     4021 taatctatat aaacaagtgt aagggtattt atacaaaaag aaaaagattt tgtttgagaa
     4081 gtaaaaccgt tgtatttatc gtcaagtagt tttacttaac aaaatcgtga aaaacactag
     4141 caatctagaa aaaatcatgg taagataatc aagtctgaga gtcatcttag aggattcttg
     4201 taaaaatggg agggaaaaac atgcgcgtaa acatcacttt agagtgtact tcttgtaaag
     4261 aacgtaacta tctaacaagc aaaaacaaac gtaacaatcc tgatcgtttg gaaaaacaaa
     4321 aatattgccc acgcgaacgc aaagttactt tgcaccgtga aacaaaataa tgtttttaaa
     4381 gttctcaatt ccttgctggt caaagaattg agactttttt tatctcaaaa catctaaaat
     4441 cataaggagc ggataatatg tttatttcca tgtgggcaca agataaaaat ggtctgatcg
     4501 gcaaaaatgg tttgttacca tggcgattgc caaatgatat gcgtttcttt cgcgaacata
     4561 caatggatcg aattttagtg atgggaagaa agacctatga aggaatgggg gatctttcac
     4621 ttccttatcg ccatatcatc gtgttgacaa cgcaaccaaa ttttcgcacg aaagaaaatg
     4681 cggaagtaat gcattcgatc gatgaacttt tgtcgtatag tcaaacgatc tcagaagata
     4741 tctatgtttc gggtggtagt cggatttttc aagaacttct tccttacact ggtcggatct
     4801 ggcgaacatt gatcgatagc acgtttgaag gcgatacgta tattgggaat attgattttt
     4861 cagacttcgc ccttatagag gagtatgagg ggataacgga tcaagaaaat ctatatgccc
     4921 atcgttttca aaaatgggaa agagtaaaaa atagggttgc ttcaaaagaa gaataaaatc
     4981 tatgcgtcta gtttgttaat aagaaaaaaa gcatctcacg actaatcgtg aagattcttt
     5041 ttttagttaa aatcaattta gtttttcttt gatatcattg accatttcct gactggcaat
     5101 aggtccgtta tatagccaac tagattcagg tccgaattgc agtacatgat tgtctgtcac
     5161 tgctttaaga ttcttccagc ttggttcatt gaacatggca gcatctttgt cactgttcac
     5221 aaggatgatg tagtcagcat ctaattcagc tagcttttca gaggaaacag cagaccagtc
     5281 agaagtggct tttttggaga cctcttccac tagatcagga acaccaaatt ccagatcgtc
     5341 gtatacgatt cttccacttg accgattttt gctgaccata aaggctgagt tgttggtcac
     5401 ccacaacact gcagctgtct tgttctcgat tttatctgct agttcttctc gagttttctt
     5461 gatggatttt tgataatcgg aaatgatttt ttctgcctct ttttcttttg ccaaagcttt
     5521 gccgacatct tttagttgtt cttcccatgt gacatctgtt ccgtttttta caacataagt
     5581 tggcgcgatt ttgctatatt gttcgtattt cccgccttcg acagtagcgg aagaagaaat
     5641 caacagcaaa tcaggctcaa agcttaacac tttctcataa ggaaggtcat aagaaatcgt
     5701 tgggacatcc tttaaatcat cttgcagata atcttgaatg gaaccactgc caacagtcca
     5761 ctgggcgata ggtttttccc ctagtgcaat cagatagtct tctaagtaac tgccgatgat
     5821 ccgtttgggc gcgtttggaa tctctacttt atgtccaagt gtatctgtga ctgtatgagt
     5881 actattggat gaagtcgtcg aattggcagt tgtagctgtt tctttatctg aagaggggga
     5941 gcaagcgctc agtactccag ccagcagcaa tatgctagat aatgtgaata cttttgtttt
     6001 taatttcatt tctttcctcc taacataatt gagaatcttt ctcattggtt attattcctg
     6061 atatttcgaa cgctgtcaac cgcttcattt ggctatagat atgaaagtag cgcttgacga
     6121 aatgttgaaa cagcgtcata attgagaatg attttcagcg aaaggatggg tgaaatgacg
     6181 agattaagag cagctgacat tgcagccggc tacgaaaaca aaaaaatcat tgaaaacatg
     6241 tctgttacga ttcctgaagg gaaaatcact agtttgattg ggccaaatgg aagtggaaaa
     6301 tctactttat tgaaaagttt tacccgtata ctacctgtga aatcaggaaa gatcattgtc
     6361 aataactctg atatctctac atacagttca aaagaacttg caaaaaaaat tacattatta
     6421 gcacaaacta gtgaacaccc tacaggtatg acagtagaag aagtagtatc ttacggacga
     6481 tacccttatc aaaaactgtt cagtggcatg gacacgcaag atcatgaagc aatcgagtgg
     6541 gcgttggatg ctacaggtct agctggattt cgtatgcggg atatcgcaac gctgtctggt
     6601 ggacaaaccc agcgtgtctg gatcgctatg gcgcttgctc aggaaacaga tatcttgatc
     6661 ttggatgaac cgacaacatt tttagatccg gcacaccaat tagagatcct acagttattg
     6721 actgaaatca atcaaatgaa acaaacaacg atcttgatgt cgattcatga cattaatcat
     6781 gcttcacgtt tttcagatta tttattagca atgaaaaacg gtaagttgat cacagaaggt
     6841 acacctgaag aagtgatcac aaaagagtgt atgaaaaaaa tatttgagat tgatgcaacg
     6901 attgtcacgc ttccaagaac aaataaacca gtttttctaa cctatgattt gttgccaaaa
     6961 gagcaggagg atcaattatg aggactatga agaaaaatag actcctgctt ttggtctgtt
     7021 ttgttctttt gattattttt tttgttgctc tttcaaatgg cgcagcgact gtgggaaaag
     7081 cagatttttg gcaggcgttg tttgattttg atagatcaaa acaaagtcac caaatcatac
     7141 gaaatattcg tttgcctcgt gtggcgggag catttcttgt agggagttgt tttgcattga
     7201 gcggtgcatt gatgcaagga gtgacaagaa atcctttagc agattcaggc ttactaggaa
     7261 tcaacgcggg tgcttcgttg gcaatggccc ttagttttgc tttttggcct aaagcttctg
     7321 atttttctgt gtttctaatg tcattggcag gttcttttgc tgtaactttg ctcgtatttg
     7381 gttttttgca gttttcgaaa atgggcatga atcctttaca gctgatttta gctggcgtag
     7441 ctattagttc tttttttact gctatcagcc aagcacttac ccaattcttt aatctgcagc
     7501 aggatcttgc tttttggttt gtaggcggag ctgccaatat cacatggaaa caattagcag
     7561 tattgtttcc tttatatata cttgctgttt tggtttcttt ttcattagga aaagcaatat
     7621 ctttgattca tttgagtgaa acacatgcat tagctcttgg aagtcatcct aaacgaacgc
     7681 ggttgattgt tttctgtcta gttgcagtgt tagcagccat tgccgtttcg ttagtcggac
     7741 ctgtttcatt tattgggctg ttggttccgc cagttgttcg cggaatggtt ggaacggact
     7801 atcgttatgt tctacctgtc tcttttttag ttggcggtgg attggttatg ttggcggatt
     7861 ttacagcgag gatgatcaat cccccttttg aaacaccttt tggattagtc attgcattga
     7921 ttggcgttcc gtttttactg atacaagtaa ggagggaaca acggtgaaaa aaatatattg
     7981 gacgttctat ttcttgcttt ttgtacttgt catcctgtct ataggtagtt taatgattgg
     8041 tacgcctttt atttacctgg atcagctttt tgccacgttc atagggaaag aatcatcttc
     8101 tttggcgctg attgttagag aatttcgagt tcctcgatta ctgatcagtt tattagcagg
     8161 agcttgtctt ggcgtttctg gttatatctt gcaaggagta acaagaaatg aattggcaga
     8221 ttctagtata ttgggcatta atgccggagc aggattttta gtgatgcttt atttaggttt
     8281 cttttcgcac tattcgctgc cgtttctatt gctagttgta gcctttctag gtggtgtttt
     8341 agctgcttgc tgtgtctatc ttgcggccta tgatcgaaat ggctttttgg gtatgaatcg
     8401 aatcttattg tccggtattg ccgttaatgc cggcctttct gcattaacat tgctatgcac
     8461 gatccaatta tctaaggaaa attatggatt tgtgaatgca tggttagcgg gatcaatctg
     8521 gggtgcgagc tggccttatg tttgggcatt gcttccatgg gcggttatct taattccgct
     8581 aggaggactt tatgcacaga ggattggatt gctatcattt ggtcaagaac gcgcgcaagg
     8641 attaggcctt aacgtggcta aaagtcaaaa aatccttttg ttgttagctg ttgctttggc
     8701 aagcggtagt gtagcggtga caggtagttt atcttttgta ggactactag ctcctcatgc
     8761 tgcgaagcta gtcgtacgta aagaaaataa ctggaccttt gttctgagcg gtttgttcgg
     8821 ttccattttt ctgcttagtg cagatatggt tgctagagtg attctaccaa gtggggaagt
     8881 acctacagga attttgattg ccctttttgg tgcgccatat tttctgtatg cactttttat
     8941 cagacaaaaa cacgcgctga acagttgaaa tttattaaaa aaggaaggcc aatcatttct
     9001 aggtcttctt tttattctat ttatgcattt atttgaaagt tcgattgcta tttttctttt
     9061 tttcctatac cattttatga gagaaggaag ggtgagtttt tggaccaatt gaaagtaaga
     9121 ttacgccgga aaggaatcga aaagctgaaa aatctggcca aaagtggcaa gaaaaaggaa
     9181 aaagaacagc gaatctataa acagctattc gccagcgagt actggaagtc agcaaaagtg
     9241 atagggacga ctttatcgaa tgaatttgaa ctggatacac agccggttat ccaacgagca
     9301 gaaaaagaac aaaagaaaat agttgtacct aaaactttgc cgaaacgtca gatggcgttt
     9361 tatgaaatgg atgaacagtc cgttcttgta cgttcgagtt ttggtgttct tgagccacaa
     9421 tcagagaagc tctatttgcc ggagatgatc gatctcctca ttgtaccagg tattatctac
     9481 aatagagccg gttatcgaat cggttttggc ggtggttatt acgaccggta tttaaataat
     9541 tttcataaaa aaacatgtag ccttgttttt cgtgaacaat tgaatgaaca atggcaaccg
     9601 gagagctttg atcagaagat ccaacaattg tttattgact aaaatgaaat gggaggtttt
     9661 catgaattat caacagcaaa taaaattgcg tgcatggtta agacgaccgt tatggaccta
     9721 tgctttttta gggatacaga cagcggtctt cctcatgatg gaattatttc cgcgacttga
     9781 gatcccttat tatgccggaa tgtatggtcc ttatcttgtt cacttcaatg aatggtggcg
     9841 tttagtgaca ccaatcttca tccattttgg actgatgcat ttcgtaatga actcactgat
     9901 actgtatttt atggggcagc aaatcgaggc aatctatggc cattggcgtt ttttcttgat
     9961 ttatatgttc agcgggataa tgggaaatac agcaagcttt gcttttaacg aagcgaatgt
    10021 tctgtctggt ggtgcgagta cttcgatatt cggtttgttc ggtgcgttgt ttatcttagg
    10081 gtttcatttt aaacataacc cagcgatcca gcagctagtc agacattttc ttttatttat
    10141 tgtgttgaca tttgtttttg gtcttctgga tacctctgta gatgtttggg gacatgtcgg
    10201 tgggctaatt ggcggattag tgttagggaa tgttttgggt ctaccgaaac aacagacaag
    10261 ctattcgata caccaaagga ttctttctac actagttttt gtatttcttt ttgtaatatg
    10321 tattttactg ggtttaaaaa aatatggttt acttgtataa tatgaaggaa gtgcttaaag
    10381 atggagagct tatatgatgt acagcagctg ttcaaaaaat ttggcatctt tatctatgtg
    10441 ggtgcacgca tctatgacat tgaattaatg actatcgaat tgcgtaatct gtatgatagc
    10501 cgtttgattg atcatgatac atacatgaca gcgtggcgca tattaaaaag agagcaccga
    10561 attgaagaaa gccgaaagaa aggaaatttg taatggacaa gaagattatt gggattgatt
    10621 taggtggaac aacagcaaaa tttgcaattt taacaccaga aggagagatt caacaaaaat
    10681 ggagcatcga taccaatatt ttggatgatg gtaagcatat cgttcctgaa atcattgaat
    10741 caatcaacca tcgtttgaat ttatataata tgaaagccga agactttatc ggaatcggca
    10801 tgggtacccc aggaagcgtg gacagcgaag ctggaacggt tattggtgcg tataacttaa
    10861 actggacaga agttcaattt gtgaaaaaac tgatcgaggc aggaacagga atcaaattcg
    10921 ctatcgataa tgatgcgaat gttgctgctt taggtgaacg atggaaaggt gctggagaaa
    10981 acgatccaga cgttgtattc gttactctag gaacaggtgt tggcggtggt atcgttgctg
    11041 gcggaaattt gattcacggc gtgagcggag caggcggaga gatcggccat atcactgtgg
    11101 atcctgaagg gttcgaatgt acttgcggta aacgcggatg tttagaaact gtgtctagcg
    11161 ctactggtgt ggtacgtcta ggtcgttact tggcagaaga atacgcagga gattcaaaac
    11221 tgaaagcaat gttagacaac ggtgaagaag taacaagtaa agatatcttt gaaatggctc
    11281 aagaagacga tccgtttgca ttgatggttg ttgatcgcgt atgcttctac ttaggtttag
    11341 cttgcgccaa cttggggaat acattgaatc cgtcaagtat tgtgttaggt ggaggagtat
    11401 cggcagcagg agaattttta cgtagccgtg tagagaaata ttttaaagaa tttactttcc
    11461 cgcaagtaag agaatcaaca aaaatcaaat tagcagagct tggtaatgaa gctggtgtca
    11521 ttggtgctgc ctcattagcc ttgaaatttg cttaaaaaaa cggaggcatt acatggtttt
    11581 atgggtaatt aatatcattt tgctattgat tgtgcttgca atcatctttt gggaagtcta
    11641 tctacgagta atggccaagc gttcagcaac gactttgaca gaagaagaat ttcgagaagg
    11701 aatgagaaaa gcacaagtaa tcgatgttcg cgaaaaagat gtgtttgatg ctgggcatat
    11761 tttaggtgca agaaacatcc cttacactgt tttgaaagat tcattaggat ctattcgaaa
    11821 agaccagcct gtctatattt acgatcaaaa gaaaagcttg agcatccgta cagcaaataa
    11881 attacgtaaa gcaggttatc aagagattta tttacttaag ggtggatatg acggatggtc
    11941 tggaaaaatc aaaagtaaaa aagtataaaa cagatgattg tatgacagct ttaaaaaccg
    12001 aatcaatcat tagattttga ccaagatcac atgaacggcg caatggatag ttgtgccgtt
    12061 catttttttt attttgtgct attatctaca tatataagct aagcgaaaag gcagaaagaa
    12121 ggaaacaact atgatcaaat taaaacgtgt ttaccttgat gctgaaaaag aggatggcta
    12181 tagaatactc gtggataggt tctggccacg aggaatttca aaagaaaaag cgcagttaga
    12241 tctgtggttg aaagaaattg cacctagtaa tgaagcacgc aaaacttttg accatgtacc
    12301 agaacgattt gcttcatttg caaagaaata ccagcaagaa ttgattttcg aagaagaaaa
    12361 gaagcaagct atttttaaac tcagtcaact tatgaaagaa catccagtca ttacttttgt
    12421 ttatggtgca aaaaatgaac atcagaacca tgctgtttta ttgaaaaaat ttatcgaaat
    12481 ggaggcatgc aaatgaaaat agggatcatt ggtgccggac ctagaggatt aagtatagca
    12541 gaaaggattc tccataataa tcaggacaga cattctcttg aactttttct tttcgatccc
    12601 aatggtcctg gaggtcgagt ttggcggttg aatcagccac cggagttatt aatgaattct
    12661 gtttctcaac aagtgacgct ttttacagat gaaacattgt ccagtggagg attggtaaca
    12721 cagggaccca acttgtttca atggagtaaa ggacctgcta cagattatat cgaacagacg
    12781 gtaaccaaaa ataagcaact aattatagaa gaaatcaatc gtctggaaaa agatcagcaa
    12841 tctactcgat gtttatacgg actctatcaa cgctggtttt tctcaaagtt gcaagaagaa
    12901 tttccagctt ctattcagtt tgtttattct ttagtaaaaa aagtcagcaa aagaaaagat
    12961 ggttatcttt tacagacaga tcagcaaagc tattctatcg atcagcttgt tttagcaagc
    13021 ggtcactggg aaaatgatga gacagaagaa gaaagagtgt gggtagacta cgcaaagaaa
    13081 aatgatttac tgtatcagct tccttctaat cctgcagatg tcgatctttc gaagataccg
    13141 cctaaagaaa atgtcttttt aagaggtcta ggcttaagtt tttttgatta tgtcggattg
    13201 tttacagtat caagagaagg gcgcttcgag aacgaaaaag gaaaacttgt ctatcatcca
    13261 tcaggcaagg aaccgattgt atacagtgga tcaagaaggg ggctccctta ttaccctcga
    13321 ggaagaaacc aaaagcaagg cggatcaatg gcatggccac ggttactaac aaaagaaaac
    13381 ctggagcaat ggcatcagtc gggatttttg actggcgaaa cattttttga ctatctgaaa
    13441 aaagacgttg agttgtttta ttacaagaaa atcatcgaag aatatcattt gccgattcca
    13501 aagaaaaatt ttgagcatga ttttctctcc ctctcttcag aggagtgtct cggaaagcat
    13561 ccagcactaa tcccttatag atgggattgg tcgtatttag aaattcctct tacctatcag
    13621 gatgagtcct ttgcagaagc tagccgagca tttattgaag ggcagataaa agaagcggaa
    13681 aaaggcaatt gcacaggagc atttgcttca acatttgatg cattaaaaga ctggcgtgat
    13741 ccaatccggc aggctattga atggcacgtt ttttctgcaa aggaatacaa agaaatacta
    13801 tggggatggt ttactcatct aaatagcttt ttaacgatcg gtccgccagt cattcggacg
    13861 aaagaattag cagcactgat agatgcgggt atttttcatt tgttcgaacc gccatttgat
    13921 attcagcaaa agcacaaccg ctatgttatt tctctacaag ataaagaaat cagcagtcgc
    13981 tatttgattg aagctcgatt gccacaaaca agcttttctc gttcaagtaa tcctgtcata
    14041 aaaaatttga aggaaaacaa acttgttcgc cctttttcat tttcagataa tacaggaagt
    14101 tatcattcag aagctataga tgttgagcga ggtacgaatc gcgtgattga tgcttcaggg
    14161 gaaatccagt ctgactttta ttgctatgga atacctgttg aaggtgtgga ttggctaact
    14221 gcagcagtag ctagacctta tacagatcca tggaatctga gacaggcaga taaaatagca
    14281 caacttatat tgactgacaa ataaaaatac gcatgatgac ttatctgttc tttcacaagt
    14341 cttcatgcgt gtttttaaat aattattaac gagaaacgcg tttgcgcatt gcttttttac
    14401 ggttttcttc gatcattgct tctttttcgt caattggatc gatcactgtt tttttcacag
    14461 aagcgaccaa accagcaacg gcagcggctg ttgccagact tcctactaat attccagata
    14521 caaatttttt cattgaatcc acctcattta ttatttgtta tatctattat ggaacaaaag
    14581 agaaaaaaag gctagtttta cgtttgtttg taaacctttg caaaaaatcc ttgaaaatgt
    14641 ttactaaatg ttttattata aatttaccat atgaatttac gaaattttac tttgtttgtt
    14701 ttcaccataa aagtaaactt cgttttttta aattatatat aatcaacaaa cacaatttta
    14761 ctaatcaaag gagtgttttt tatgacaatt ttagtactag gcggagccgg ttatatcggt
    14821 tcgcacgctg tagaccagct agttcaaaag gggtatcaag tagctgtggt agataatttg
    14881 ctcacaggtc acaaacaagc agtacatcca gatgctcatt tttatgaagg agacatccgg
    14941 gataaagaat ttctacgttc cgtatttgaa aaggaaccga ttgaaggagt aatccatttt
    15001 gctgcaagct ctttagtagg agaatctgtc gaaaaaccat tgatgtattt caacaacaac
    15061 gtatacggta tgcaaatctt acttgaagtc atgcacgagt tcaatgtgaa taaaatcgtg
    15121 ttctcttcta ctgcagcaac ttacggagaa ccaaaagagt ctccaatcac agaagataca
    15181 ccagctaatc ctaaaaatcc gtatggtgaa agtaaattga tgatggaaaa aatgatgaag
    15241 tggtgtgacc aagcatatgg catgcgctat gtcgctttgc gctacttcaa tgtagccgga
    15301 gcaaaagcag atgcatctat cggagaagat catacaccag aaacgcatct tgtcccaatc
    15361 attttacaag tagctcttgg tcaaagaaaa gcattggctg tttatggaga cgattatgat
    15421 acaccagatg gaacatgtat cagagattat gttcaagtag aagatttgat tgctgcacat
    15481 attttagctt tggaatactt gaaagaagga aatgaaagca acttcttcaa tttaggcagc
    15541 agcaaaggat actcagtcaa ggaaatgcta gaagcagcta gagaagtgac aggaaaagaa
    15601 attccagcag aaatagcacc aagaagagct ggagatccta gccgattagt cgcttcaagt
    15661 gaaaaagcga gagaggttct gggttggaaa cctgaataca cagatatcaa agcaattatc
    15721 aaaacagcat gggattggca tgtcagccat ccaaatggtt atgaagaata ggggaatgag
    15781 atatgtcaat cagtcaaacg atttgtgatt ttgccacact tgcgatcgaa gctggcggat
    15841 ggatggaaat ggaccgctta tatttacaga atcgtctttt agggatggta ggagaagatg
    15901 ctctagaaag cattgaagta agaccagtta aggaagaatc tgtagactta ttggatcagc
    15961 ttgtcgctaa ggcaagagaa aatggtgttc tttcagaatt gccagcagaa caagagattt
    16021 ttgaagcgca attaatggat tttctcacac cgccaccttc agtcgtcaat gcattctttg
    16081 cacagcacta ttcgaaaagc ccagaggaag caactgagta tttctttaat ttaagtaaga
    16141 aaaatgacta catcaaaaca agagccatcg caaaaaacat caaatttccg gcagagtcgg
    16201 agtatggtga actggaaatc acaatcaatt tatctaagcc agaaaaagat ccaaaacaaa
    16261 tcgcagcaga acgcgaggcg gtaaaagtag actatcctac ctgcatgctt tgcatggaaa
    16321 acgaaggata tagaggtcgc ttgaactatc cagcccgcac caatcaccgg attattcgaa
    16381 tgaatctaga cggtgaaagc tggggattcc aatattctcc ttatgcttat tacaatgaac
    16441 attcgatcat tttatcagaa caacaccgcc cgatgaaaat cacaaaggaa acatttgcta
    16501 gactgcttcg gattactgaa gtcttgcctc attactttgt tggttcgaat gctgacttgc
    16561 cgattgttgg aggatcgatt ctttctcatg atcattatca ggcaggacgt catgaatttc
    16621 ctatggcaaa agcaccgata gaaaaactta tcaaactagc agattatccg caagtcatcg
    16681 caggagtcgt gaaatggccg atgtctgtga tccgtttgca atcaacagag aaaaaattgt
    16741 tgattgaagc agctaactca gtattggaaa agtggaaagc atattcggat gaaacagtat
    16801 ctatcagagc tttttcagca gatggtacac cacaccatac gattacgcca attgcacgaa
    16861 gaagggacaa ttactttgag ttggaccttg tccttcgaga caacaatgtt tcagaagaac
    16921 atcctgatgg gatttttcat ccacataaag atgtccaaca tatcaaaaag gagaacattg
    16981 ggttgatcga agtaatgggg cttgctgttt taccacctcg tttgaaacca gaactagtag
    17041 aagtagagca tttcttatta ggagaagaaa accagatggc tgaatatcat cgtccgtggg
    17101 cagaacaatt aaaaaaagaa catcctgatt tgacgaaaga tacggtcacc gatgtcgtac
    17161 aaaaatctgt aggtcaggtt tttgcacgtg tactggaaga cgctggagta ttcaagcgag
    17221 atgaaaaagg acaggcagcg tttgaacgat ttactgcaat tttataaacg taaaaaagga
    17281 ggagagacaa atggcaacaa ttaaagacat tgcgcagttg gcaggagttt ctcctgctac
    17341 tgtttcaaga gttctcaact atgatcctga tctttccgtt ggcttagcca caaagcaaaa
    17401 aatttttgaa gcagcagaac gcttaaatta tacgaaacat cgtaaaaatg cagcaaatac
    17461 taagcaaacg cttcgcctga tccagtggta cgacgatacg gaagagcttg aagatcttta
    17521 ttatttatct attcgtctag gaatcgaaaa aaaggcagaa gaagcaaacg ttactttatt
    17581 gaaagaaacg tgggattcca ttagtgatct tccgacagat ggaacgatcg cactaggaaa
    17641 attcgacgaa gctcagttat tagaaatgaa aggatcccaa aatgccttgc ttttggtaga
    17701 ttctgacggc acttctcaag gaatcgactc tctagttgtc gactttaagt caagtgttca
    17761 aaaagtcatt gaacattttg tctcccataa agcgcaacat atcgctatgc ttgctggaac
    17821 agaatatacg aagaaaaacc atcagcggct aaaagatccc agaattatgg catttcaaca
    17881 agttttggca gaaaaaggct acgacgaagg tccaattatc gaagcagatt tttctgtcga
    17941 gtcaggatat caagcagtga agcagtattt aaaaacgaat ccgcatgttc ctgatgcgct
    18001 ttttgcagct aatgatgcat tagcgattgg cgcactgaaa gcgattcaag aaaaaggttt
    18061 agtggtacca gaagatattt ctgtgatggg tttcaatgat gtcagtgtag cgaaatatgt
    18121 aacgccctct ctttctactg taaaagttta taccgaatgg atgggagagc tagcagtgga
    18181 gacgattctt caattaagta attccaaagc accggtttca agaaagatta ttgtggaaac
    18241 agagttgatt attcgagaat caacgaaata
//
LOCUS       DAZAJZ010000056        17770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000056 DAZAJZ010000000
VERSION     DAZAJZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.056"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1252)
                     /locus_tag="ACG86D_001846"
     CDS             complement(44..1252)
                     /locus_tag="ACG86D_001846"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN3140984.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(1364..1696)
                     /locus_tag="ACG86D_001847"
     CDS             complement(1364..1696)
                     /locus_tag="ACG86D_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN3140985.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1844..2719
                     /locus_tag="ACG86D_001848"
     CDS             1844..2719
                     /locus_tag="ACG86D_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3140986.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(2801..3382)
                     /locus_tag="ACG86D_001849"
     CDS             complement(2801..3382)
                     /locus_tag="ACG86D_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN3140987.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3388..4647)
                     /locus_tag="ACG86D_001850"
     CDS             complement(3388..4647)
                     /locus_tag="ACG86D_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN3140988.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(4649..5695)
                     /locus_tag="ACG86D_001851"
     CDS             complement(4649..5695)
                     /locus_tag="ACG86D_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3140989.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(5930..6205)
                     /locus_tag="ACG86D_001852"
     CDS             complement(5930..6205)
                     /locus_tag="ACG86D_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN3140990.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(6448..7758)
                     /gene="der"
                     /locus_tag="ACG86D_001853"
     CDS             complement(6448..7758)
                     /gene="der"
                     /locus_tag="ACG86D_001853"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN3140991.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(7940..9169)
                     /gene="rpsA"
                     /locus_tag="ACG86D_001854"
     CDS             complement(7940..9169)
                     /gene="rpsA"
                     /locus_tag="ACG86D_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN3140992.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9304..9984)
                     /gene="cmk"
                     /locus_tag="ACG86D_001855"
     CDS             complement(9304..9984)
                     /gene="cmk"
                     /locus_tag="ACG86D_001855"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN3140993.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10033..10668)
                     /locus_tag="ACG86D_001856"
     CDS             complement(10033..10668)
                     /locus_tag="ACG86D_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN3140994.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(10734..12137)
                     /locus_tag="ACG86D_001857"
     CDS             complement(10734..12137)
                     /locus_tag="ACG86D_001857"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN3140995.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(12124..13155)
                     /locus_tag="ACG86D_001858"
     CDS             complement(12124..13155)
                     /locus_tag="ACG86D_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3140996.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13186..13407
                     /locus_tag="ACG86D_001859"
     CDS             13186..13407
                     /locus_tag="ACG86D_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN3140997.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(13572..14309)
                     /locus_tag="ACG86D_001860"
     CDS             complement(13572..14309)
                     /locus_tag="ACG86D_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN3140998.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(14462..15802)
                     /locus_tag="ACG86D_001861"
     CDS             complement(14462..15802)
                     /locus_tag="ACG86D_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3140999.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16039..17562)
                     /locus_tag="ACG86D_001862"
     CDS             complement(16039..17562)
                     /locus_tag="ACG86D_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN3141000.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5020 a         3791 c         2997 g         5962 t
ORIGIN      
        1 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
       61 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      121 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      181 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      241 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      301 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
      361 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
      421 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
      481 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
      541 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
      601 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
      661 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
      721 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
      781 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
      841 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
      901 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
      961 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1021 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1081 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1141 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1201 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1261 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1321 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     1381 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     1441 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     1501 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     1561 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     1621 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     1681 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     1741 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     1801 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     1861 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     1921 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     1981 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2041 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2101 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2161 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2221 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2281 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     2341 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     2401 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     2461 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     2521 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     2581 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     2641 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     2701 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     2761 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     2821 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     2881 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     2941 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3001 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3061 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3121 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3181 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3241 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3301 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     3361 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     3421 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     3481 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     3541 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     3601 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     3661 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     3721 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     3781 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     3841 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     3901 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     3961 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4021 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4081 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4141 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4201 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4261 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4321 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     4381 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     4441 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     4501 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     4561 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     4621 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     4681 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     4741 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     4801 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     4861 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     4921 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     4981 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5041 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5101 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5161 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5221 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5281 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     5341 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     5401 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     5461 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     5521 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     5581 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     5641 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     5701 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     5761 aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg tgttttctat
     5821 gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc taggaaataa
     5881 aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct tatttaacag
     5941 cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga atttcgattt
     6001 cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact tcaaagttac
     6061 caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata gttgaaaata
     6121 cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa gcaacttttt
     6181 cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga gatatgatag
     6241 aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa atattgatta
     6301 acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg tttttaccaa
     6361 ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt tttcttttga
     6421 aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa ttcggatgga
     6481 cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt cataagaaaa
     6541 atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga cagcgacttg
     6601 tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat tgattgctac
     6661 agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct ggttcatact
     6721 tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg cagaaacaaa
     6781 aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt cgaaatcacg
     6841 cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa taattccacg
     6901 accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc cttcttcagc
     6961 attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac gcatcacgct
     7021 gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg tatctatcat
     7081 taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc gggtcgttcc
     7141 ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca aagaggattt
     7201 accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct cttcttcagt
     7261 ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta gtccatggct
     7321 tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga tatcgtttct
     7381 catttctgga ttatctactt tattgactgc taaaatcact ggtttattac ttctgtataa
     7441 aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga ctacacaaat
     7501 gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat ccataaaagg
     7561 ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac ctaaccattc
     7621 accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa tcgagatacg
     7681 ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc caacgattgc
     7741 aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac ttagtttaac
     7801 gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt ttattcctta
     7861 ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc tttcatctca
     7921 gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct tctacttctt
     7981 ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct tctggtaatt
     8041 cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct aaagctttga
     8101 tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact ttcacttcgt
     8161 caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt tgagagatat
     8221 ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt gtcaaacgtt
     8281 taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct gaccaaggtc
     8341 caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg atagacaaca
     8401 ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct acatgactat
     8461 gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca aatgcgccaa
     8521 aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata gttgaaagaa
     8581 tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat aagatcaaac
     8641 gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct ttatagtctg
     8701 aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca cgtacaccga
     8761 cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg accttgccct
     8821 cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg cgttttgaaa
     8881 gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact aaatctaata
     8941 catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat aattcttttg
     9001 ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt ttgtcttcaa
     9061 tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg cttttcattg
     9121 cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata acacttgtcc
     9181 tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga ttattcaaaa
     9241 aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat tttttttctt
     9301 aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg caacgacttc
     9361 tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct tcagcggaga
     9421 gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca gtgtttcaag
     9481 atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac gctcacttac
     9541 gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc cgatgtctct
     9601 tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa ctaattcttc
     9661 tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg gttgacggat
     9721 ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa
     9781 agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa aagatacatt
     9841 gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg tataaatata
     9901 attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag caggcccgtc
     9961 gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa aagccgcaaa
    10021 gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa agttgtttgg
    10081 atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct cagccacttg
    10141 ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg
    10201 tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct cacttgagga
    10261 agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat cttgagacac
    10321 agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg actcctcttt
    10381 tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag ttgctgctgt
    10441 tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa tagcgataca
    10501 taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct tttgttgtct
    10561 ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt cccatggttc
    10621 ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg ttttacctcc
    10681 taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt
    10741 tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg ttcagtctta
    10801 gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc acaacaattc
    10861 tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct gcggcagttt
    10921 tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt ttcctgacgt
    10981 ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc catccatttt
    11041 ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag ctcaaaactt
    11101 tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc cccactttga
    11161 taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca
    11221 tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc agatttatcg
    11281 atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt tacaaactgc
    11341 tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc aactgtaaaa
    11401 cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca ataaatgatt
    11461 gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg ctgcgttttc
    11521 cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt tggtggtcga
    11581 tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc cgttagtgcc
    11641 agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata ttctggacgg
    11701 aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa cgcaatattt
    11761 tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact caaaaacaaa
    11821 aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga aagtaaacta
    11881 ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt
    11941 agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc cgtcaattga
    12001 tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc tccatcaagc
    12061 aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa atattttttt
    12121 aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg
    12181 aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac
    12241 ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg
    12301 aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa
    12361 atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat
    12421 catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga
    12481 atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata
    12541 aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt
    12601 tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg
    12661 caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc tttcactaag
    12721 ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat
    12781 acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc
    12841 ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa
    12901 catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg
    12961 ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt
    13021 atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc
    13081 cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa
    13141 aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt
    13201 aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt
    13261 ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag
    13321 ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga
    13381 gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt
    13441 tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat
    13501 tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg
    13561 acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt
    13621 tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca
    13681 aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc
    13741 ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa
    13801 acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat
    13861 acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca
    13921 cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc
    13981 gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag
    14041 ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca
    14101 ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag
    14161 taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac
    14221 gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag
    14281 ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc
    14341 tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca
    14401 gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt
    14461 tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac
    14521 tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg
    14581 caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg
    14641 gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac
    14701 tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt
    14761 tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc
    14821 tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg
    14881 ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt
    14941 cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa
    15001 ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac
    15061 caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt
    15121 cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg
    15181 ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata
    15241 agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc
    15301 cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa
    15361 agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat
    15421 acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt
    15481 cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa
    15541 cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt
    15601 aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt
    15661 tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat
    15721 ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt
    15781 gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca
    15841 aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc
    15901 aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac
    15961 ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt
    16021 acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac
    16081 aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc
    16141 aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga
    16201 tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg
    16261 tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat
    16321 agggatatag aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc
    16381 ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat
    16441 accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa
    16501 ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac
    16561 taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc
    16621 tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt
    16681 gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat
    16741 aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc
    16801 ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg
    16861 atctttttcg atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat
    16921 aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga
    16981 agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac
    17041 taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag
    17101 taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa
    17161 taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga
    17221 agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac
    17281 caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg
    17341 tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg
    17401 ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc
    17461 agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata
    17521 cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt
    17581 tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa
    17641 aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct
    17701 cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta
    17761 taaacaaaca
//
LOCUS       DAZAJZ010000057        17740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000057 DAZAJZ010000000
VERSION     DAZAJZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.057"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..841
                     /locus_tag="ACG86D_001863"
     CDS             29..841
                     /locus_tag="ACG86D_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN3141001.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            838..1638
                     /locus_tag="ACG86D_001864"
     CDS             838..1638
                     /locus_tag="ACG86D_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN3141002.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1799..3067
                     /gene="rseP"
                     /locus_tag="ACG86D_001865"
     CDS             1799..3067
                     /gene="rseP"
                     /locus_tag="ACG86D_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN3141003.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3135..4844
                     /locus_tag="ACG86D_001866"
     CDS             3135..4844
                     /locus_tag="ACG86D_001866"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN3141004.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5056..9408
                     /locus_tag="ACG86D_001867"
     CDS             5056..9408
                     /locus_tag="ACG86D_001867"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN3141005.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9552..10025
                     /gene="rimP"
                     /locus_tag="ACG86D_001868"
     CDS             9552..10025
                     /gene="rimP"
                     /locus_tag="ACG86D_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN3141006.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10049..11224
                     /gene="nusA"
                     /locus_tag="ACG86D_001869"
     CDS             10049..11224
                     /gene="nusA"
                     /locus_tag="ACG86D_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN3141007.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11246..11539
                     /locus_tag="ACG86D_001870"
     CDS             11246..11539
                     /locus_tag="ACG86D_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN3141008.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11536..11847
                     /locus_tag="ACG86D_001871"
     CDS             11536..11847
                     /locus_tag="ACG86D_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN3141009.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11860..14166
                     /gene="infB"
                     /locus_tag="ACG86D_001872"
     CDS             11860..14166
                     /gene="infB"
                     /locus_tag="ACG86D_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN3141010.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14193..14540
                     /gene="rbfA"
                     /locus_tag="ACG86D_001873"
     CDS             14193..14540
                     /gene="rbfA"
                     /locus_tag="ACG86D_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN3141011.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14648..15152)
                     /locus_tag="ACG86D_001874"
                     /pseudo
     CDS             complement(14648..15152)
                     /locus_tag="ACG86D_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15317..17680
                     /locus_tag="ACG86D_001875"
     CDS             15317..17680
                     /locus_tag="ACG86D_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFN3141012.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6048 a         3148 c         3908 g         4636 t
ORIGIN      
        1 gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata
       61 tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc agcacattgc
      121 catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg
      181 ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt
      241 gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt
      301 aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag aattgattaa
      361 agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac atacgcaaga
      421 tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat tgaattttgc
      481 tttgaattat ggaagtagag cagagatcgt gactgctgtc caagaaattg ccgaagaagt
      541 agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta agcatttaat
      601 gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta caagtggaga
      661 agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt attttacaaa
      721 agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa
      781 tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg
      841 aaacaacgtg tgatcacagc agctgtggcg ctagtcttgt ttattccgat tatatggatc
      901 ggaggcatag cggttgagct tacagcagca atcttagcgg tagtaggagt ttacgagctg
      961 ttccggatga aaggattgac actcttaagt tttgaaggag ttttatccgc cgttggtgca
     1021 gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa tgtaagtaca
     1081 tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat ttctaaaaat
     1141 acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt gggaataggt
     1201 ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt tggtctattt
     1261 attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg tcagcataag
     1321 ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg tatcggtagt
     1381 gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact attcaatcat
     1441 ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca atttggtgat
     1501 ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa tatcttacca
     1561 ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat
     1621 ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat aaaagacgca
     1681 ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat ggtaaaatta
     1741 tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat
     1801 gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc atgagtttgg
     1861 tcatttcttc tttgcgaaac gatcaggaat cctcgtccgc gaatttgcta tcggtatggg
     1921 tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc gcctattacc
     1981 tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa tggcaccagg
     2041 aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa atttaagtaa
     2101 gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt tagaagatga
     2161 gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct accctgttga
     2221 tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc caaaagacgt
     2281 acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg gacccatgaa
     2341 taactttatt ttggcaatcg tgttattcat cattctggca tttatgcaag gtggtgttca
     2401 agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg cagaagcagg
     2461 cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt catggaatga
     2521 tctgacaaca gtcattacga aaaatccagg taaaacatta gactttaaga tcgaacgaga
     2581 aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt
     2641 tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga tcattggagg
     2701 gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat cactatttac
     2761 aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt cttcagaagc
     2821 agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa tgaatctcgg
     2881 aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct tgaatatttt
     2941 tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga cattagctgg
     3001 ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc aacgattctt
     3061 tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt tgaggaaata
     3121 aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc gaagttccaa
     3181 atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc cgccaagtat
     3241 cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa ctgaaacgca
     3301 ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct gccatcttgc
     3361 cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt
     3421 tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa acttttactg
     3481 atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt tatcaaatcc
     3541 aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga agagagttta
     3601 tcatgaaaga tgcatattct ttccatgcaa cggaagaaag tttggacgag acatataaag
     3661 attacgaaaa agcctatacc gaagtattca agcgttgtgg cttagaattt cgctcgatca
     3721 tcggagacgg cggcgcaatg ggcgggaaag actcaaagga attcatggcg atctcagaaa
     3781 tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac ttggagatgg
     3841 ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag
     3901 ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt gaacctcaaa
     3961 aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt ttggtacgtg
     4021 gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac tttttagaag
     4081 aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct gtcggaccag
     4141 tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat ttagcgaatg
     4201 ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca aaacgcgatt
     4261 tcactgtgct ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct tcaccagaca
     4321 acaacggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatattc aaaatcggca
     4381 ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga gaaaaatttg
     4441 tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac
     4501 attcagatga acaaggaatc aattggccaa aaggaattgc gccatttgat ctgcatatcg
     4561 tccaaatgaa tctaaaagac gaataccaaa caagcctgac gaatgaacta gaagaagcga
     4621 tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca ggagtgaaat
     4681 tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg
     4741 atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag
     4801 aattggcgaa tactttacca atcctcttga accaaggcga ataataagcg ttaaaacaca
     4861 gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct tttaaaattg
     4921 gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa aaacgagtat
     4981 actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt ttctcatcag
     5041 aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt ggaacaaatc
     5101 caactagagg aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat ggatagtgta
     5161 gtggtccatc gtaaatcgcg tttatgggaa tttacgctac atttttccaa aattttgcct
     5221 attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga tattgcacaa
     5281 gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt gcaagattac
     5341 tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca agtattgaaa
     5401 acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag tacaggagct
     5461 atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt cagttacgga
     5521 ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg tgtattgaaa
     5581 ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc agctgaaagt
     5641 ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga
     5701 cccatccagc taggacgaaa tattccagcc gatgaaccaa cgacaccaat gatcagtatc
     5761 gttgaagaag aacgaagagt cacacttgag gggtacgtat tcgacaaaga agtcagagaa
     5821 ctacgttcaa aacgcaagat cttgactttg aaaatcacag attatacttc atcatttatc
     5881 gtcaaaaaat tctcgaacgg cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt
     5941 agctggttaa aggtacgagg aagtattcaa gaagatacgt ttgtccgtga tttggtcatg
     6001 aatgcgcaag atatcataga agtcaaacat acacctcgca aagattatgc tcctgaaggg
     6061 gaaaaacgag tagagcttca tgttcatagc aatatgagta cgatggatgc aacaaacagc
     6121 atctctgatc ttgtggcaca agcaggaaaa tggggacacc gagctatcgc cattacagac
     6181 catggtggtg cgcaagcctt tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa
     6241 atcttatacg gcgtggaagc gaatgtcgta gatgatggtg tcccaattgc ttataatgat
     6301 gcacatgaag cattgagtga agccacatac gtcgtatttg acgtggaaac gacaggattg
     6361 tctgcagtat atgacacgat catcgagcta gccgcagtca aaatgtataa aggaaatgtg
     6421 atcgaaagct ttgatgaatt tatcgatcca ggacatccat tgtcacgaac aacggtagat
     6481 ctgactggta tcacggatga aatggttcga gggtcaaagt cggaagaaga agtattgcgg
     6541 atgttccttg aattttcaaa agatacaatt ttggtagccc acaacgcggc ttttgatatg
     6601 gggttcttga atactagcta cgcaaggtat ggtattcctg aagcggccaa tccagtcatt
     6661 gatacgttgg aattagctag atatctgtat cctcaattca aacgtttcgg attaggtgtg
     6721 ttatctaaaa agtttggtgt tagcttggaa cagcatcacc gagcaatcta tgatgctgaa
     6781 gcaactggtc acttagcatg gatctttgtg aaagaggcaa tggacaatca taacatgctt
     6841 tatcatgatc agctaaatga gcatatcgga gaaggagatt cttacaaaag ggctcgacct
     6901 ttccatgtaa cgattttagc aaaaaatcag gcaggactga aagatttgtt caaactgatt
     6961 tcaatgtcga atgtcgaata cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa
     7021 aagatgcgtg aaaatctatt gatcggctca gcttgtgata agggagaaat attcgaagcc
     7081 atgatgcaaa aaggggtaga agaggcaaga aaccgagcaa aattctatga ttatattgaa
     7141 gttatgccaa aagcggtcta tgcgcctttg atcgagcaag aattggtaaa aaatgaacat
     7201 gatttggaag aaattatcca aaatttggta gaaatcggaa aaagtttgga taaaatcgtt
     7261 gttgcaacag gtaatgtaca ttatctgaac gaagaagatg ccatttaccg aaaaattttg
     7321 atcaattcta tgggcggagc gaatccgttg aatcgtcata gtttgccgga tgtccatttc
     7381 cgaacaaccg atgagatgct aactgcattt cactttttag gggaagaaac agcgaaagag
     7441 atcgtagtag aaaatacgaa taaaatcgca gatatctgcg aagaagtgat tccagtcaaa
     7501 gatgaacttt atacaccgaa gattccaggt tcagaagatg aaatatcaga attgagttac
     7561 acaaaagcaa aacaaatgta cggagatccg ttacctgaga tcattcaaaa acgattagaa
     7621 aaagagttga attctattaa tggtaatggc ttctcggtta tttatctgat cgcccaaaaa
     7681 ctagtgcata aaagtaatga agatggctat ctagtaggtt cacgtggctc tgttggctct
     7741 agctttgttg cgacgatgac aggaatcact gaagttaatc cattagcccc tcattattat
     7801 tgtccagagt gccaatattc cgagtttttt gaagatggta cgtatggttc tggatttgac
     7861 atgccagaaa aacaatgtcc aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt
     7921 ccatttgaga catttttagg attccatgga gacaaagtgc ccgatattga tttgaacttc
     7981 tcaggtgatt atcaagcaga agcccataac tatacgaaag tattgtttgg cgaagactat
     8041 gtttaccgtg ccggaacgat tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa
     8101 ggatacgaaa gggacaataa tctgcagttt cgaagtgcag aagtcgacag attagcaaaa
     8161 ggcgctactg gagtcaaacg gacaaccggc cagcatccag gggggatcat cgttattccc
     8221 gattatatgg atgtctatga ttttacgccg atccaatatc cagcagacga tcaaaattca
     8281 gaatggaaaa cgactcattt tgatttccac tcgattcacg ataatgtatt gaaattagat
     8341 attctagggc atgatgaccc aactgttatt cggatgcttc aagatttatc agggatcgac
     8401 ccacaaacga tccctacgga tgacccggaa gtgatgcgga tatttgccgg tcctgaagta
     8461 cttggagtaa gtcaggaaca aatttattcc aaaacaggga cattaggtat tccagaattc
     8521 ggcactcgtt ttgttcgagg gatgttagaa gaaacgcatc ctacaacttt tgcagaacta
     8581 ctgcaaattt ccggactatc tcatggtaca gacgtatggt taggaaatgc ggaagaattg
     8641 attcgacgag gagatgcgac actggcggaa gtaatcggct gtcgtgatga tattatggtt
     8701 tatttgatcc atgctggatt agatagcggg atggcattta aaatcatgga aactgttcgt
     8761 aaaggacaat ggaacaagat ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa
     8821 gaaaataatg tgcccgactg gtatatcgat tcttgttcaa aaatcaaata tatgtttccg
     8881 aaagcccatg ctgcagctta cgtattgatg gctcttcgtg tcgcttattt caaggtttat
     8941 ttccctattt tgtattactg tgcatacttt tctgtacgtg cagacgactt tgatctagtc
     9001 tccatgtgca aaggaaaaga tgctgtgaaa caagcaatga aagaaatcac ggacaaggga
     9061 ttagatgctt ccgtaaaaga aaagaatcaa ctgacagtgc tggagttagc caatgaaatg
     9121 ctggaacgag gattcaaatt tggaatgatt gatttgtata aatcggatgc cgttaacttt
     9181 gttattgaag gtgatacgtt gatcgcacca tttagagctg tcccaagttt gggaaccaac
     9241 gtggcgaaac aaatcgttga agcaagaaaa gacggtccat ttttatccaa agaagatctt
     9301 gccacccgcg gtaaagtttc aaaaacattg atcgaatata tgaacgacaa tggcgtattg
     9361 aaagatctac cagatgaaaa tcaattatcg ttattcgata tgctataagc atctgttttc
     9421 ctgtctgctt gtaattttaa gcagagtatg gtatagttct tataggtaat gatactaact
     9481 gtgagtgagc ggatttttcg ctcactcttt ttaatggaat tacgcactta atttttaagg
     9541 aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag
     9601 atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct
     9661 tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg
     9721 agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc
     9781 tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg
     9841 cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg
     9901 aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac
     9961 gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat
    10021 tttaatcacg gagaggaatc agaaaaaaat gagtaaagaa atgttaaatg cgttagacgc
    10081 tttagaagct gaaaaaggga tctcaaaaga aatcgtgatc gatgctttag aagcagcatt
    10141 ggtttctgca tacaaacgtc actatggcca agcacaaaac gtcgaagtgg aatttgatca
    10201 gaaaaaagga aagatccatg tctatgcggt aaaagaagta acggaagaag tgatggattc
    10261 acagttggaa gtctcattga aagatgcact tttaatcaat ccagcatatg agatcggtga
    10321 tacgatccgt tttgaagtaa caccaaaaga ttttggacgg atcgctgcgc aaacggctaa
    10381 acaagttatt ttacagcgtg tacgtgaagc agaaagaacg attatttata acgaattcag
    10441 tgcttatgaa aaagacatca tgcaaggaat cgtcgaacgc caggataaac gttatattta
    10501 tgtcaatctt ggtaaaattg aggcagttct ttctaaacaa gatcaaatgc caaatgaatt
    10561 ttatcaacca catgatcgaa tcaaagttta tgtttcccga gtagaaaata cgtctaaagg
    10621 tccgcaagta tttgttagcc gcagccatcc agacctattg cgtcgcttat ttgaacaaga
    10681 agtaccagaa gtctatgatg gaatcgttga aattgtcagc atcgcccgtg aagctggcga
    10741 tcgttcaaaa gtggcagttc gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt
    10801 cgggccaaag ggacaacgag ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga
    10861 tatcgtcgaa tggaatgaag atccagctgt attcatcgca aatgcattga atcctgcaca
    10921 agtcatggat gtgatcttcg atgagacaaa tccaaaggcc tgcacagttg tcgttccaga
    10981 ctaccaatta tcgctagcaa tcggtaaacg cggacaaaat gcacgtttag ctgctaaatt
    11041 gactaaccat aaaatcgata tcaaatcaga atctgacatg acggaatttt atgaaaatca
    11101 agaacaacaa aaagaaacag aagaactgca tgatgaagca atcgttcagt cagatttaac
    11161 agacgatgaa tatgaaacaa ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc
    11221 ttaactttag taaggaggca aagagatgaa acaaaggaaa attcctttac gcaaatccgt
    11281 ggtttcaggt gaaatgaaac ctaaaaaaga gatggtccga atcacccgtt caaaagaagg
    11341 ggaagtatcg atcgatccaa ctggaaaaat gcctggacga ggagcgtatg tctcacttga
    11401 gccggaagaa gtacaacaag cttgggataa aaaaattctc gatcgagtac tcgaaacaac
    11461 attaacagat gagttttatc aagaacttct tgactatgtc actcaccaaa aagcacgtaa
    11521 agagttattt ggaaaatgaa tacagttaat cggcaaaaag ccatgaatct catcggtcta
    11581 gcgatgcgcg cgggaaaact aatcacgggt gaagaactga ccattgctga aatccgcaaa
    11641 caaaaagcaa aattggtgtt tgtggcaacc gatgcaagtg agaatacaag aaaaaagata
    11701 aaagataaga gttcatatta tgaagttcct tgctttgagc tattttctga agcagaaatc
    11761 acgcagatga tcggaaaacc tcgcaaggta ttcggaataa tggatgccgg atttgcgaag
    11821 aagaccaagg agctaattga aggttaggaa ggtgattgca tgggcaacaa aagaatctat
    11881 gaattagcaa aagaactcaa aaaatctagt aaagacgtcg tggaaaaagc acaacaactt
    11941 ggcatcgatg tgaaaaacca tatgggtgcg attactagca gtgatgagaa gaaattgcaa
    12001 caagcgttca aaaacccaag cacgaaacaa caagctgcac aacaagcaag cagaaaacca
    12061 gccaatcaac agccgaacga tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat
    12121 aaaaataacc gcagttatca agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa
    12181 aaaccaacaa caaaccaaaa taatacggga aataccagca gccaaaatcg tcaaggaacg
    12241 agccaaggcg gcaatcaaaa ccgtactaac aataacaaca aaccaaacaa taacaatcgc
    12301 ggcaaattta ataataataa ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag
    12361 caacaatcaa ctaaaccagc tgtaccacca cgcaaattcc gtgaattgcc agatgtatta
    12421 gagtatactg aaggtatgaa cgtagcagat attgcgaaaa aaattcatcg tgagccagca
    12481 gaaatcatta aaaaattatt tatgcttggt gtcatggtca atcaaaacca agcactggat
    12541 aaagatacga ttgaactgtt agcgacggac tatggcatgg aaccacaaga aaaagtccaa
    12601 gttgacattg ctgatatcga taaattcttc gaggcagaag aagtcgatcc agataaatta
    12661 gtctcacgtc cgccagtagt aacgattatg ggacacgtag accacgggaa aacaacctta
    12721 ttggatacat tgcgccattc acgtgtaaca agcggcgaag ccggcggaat cacacagcat
    12781 atcggtgcct accagatcga tattgatggt aaaccaatca ctttcttgga tacaccagga
    12841 catgcggcct ttacaagtat gcgtgcacgt ggagcaagta tcacagatat cacgatctta
    12901 gttgttgctg cagatgatgg cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa
    12961 gctgcaggcg ttccaatcat cgtcgctgta aataaaattg ataaaccagg tgcaaatcct
    13021 cagcatgtta tgcaagaatt aagtgagtat gaattaattc ctgaagcatg gggtggagaa
    13081 acgatttttg ttgagatctc agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg
    13141 atcctgttag tcgcagaagt agaagattta aaagcagatc caacacaacg cgcaatcgga
    13201 acagtgatcg aagcacgtct tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa
    13261 gaaggaacac ttcacgttgg tgacccaatc gttgtcggta atacttacgg acgtgtccgt
    13321 gtaatgacaa acgatcttgg ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa
    13381 atcactggct tgaatgatgt acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa
    13441 aagacagcac gtgcagcggg tgaagaacgc gcaaaacgtg cattattgga aaaccgtgct
    13501 gccacaagcc gtgtgacttt ggacaactta tttgaaagcc tgaaagaagg cgaattgaaa
    13561 gaagttaacg taatcatcaa agcagatgta caaggttcag cagaagcact ggctgcatca
    13621 ttgaagaaga ttgatgtgga aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca
    13681 atcaatgaaa gtgatgtaac cttagcagca gccagcaacg ctattatcat cggtttcaac
    13741 gttcgtccta caccgcaggc aaaagtgcaa gctgatactg aagaagtcga tattcgtctt
    13801 caccgtatca tctataaagc catcgaagaa atcgaaactg cgatgaaagg gatgctagac
    13861 cctgaatttg aagaaaaaat caccggtcaa atgatcgttc gtgaaacctt caaagtttct
    13921 aaagttggta cgattgcagg tgcctatgta acagaaggat atattcgtag agatagcggt
    13981 gtccgtgtaa tccgtgacgg aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga
    14041 ttcaaagatg acgtgaaaga agtcaaaatg ggttatgaat gtggtgcgat gattgaaaaa
    14101 ttcaatgaca tcaaagtaga tgacgtaatt gaaggattta tcatggaaga agtcaaaaat
    14161 gactaaaaac tgataaaagg aggccaatca ttatggctaa ctaccgtgat cgccgagtcg
    14221 gtcaagaaat cttaaaagaa gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg
    14281 tggagaacgt aacgatcaca gatgtgaaag ttacggggga cttacagcaa gcaacgatct
    14341 attacagcat tttatctgac ttagcatcag ataagaaaaa agcacaagat ggattgaata
    14401 aagcatcagg attgattcgt cgagagttag ggcacaatct aagtatttat aagacacctg
    14461 aattgttctt tgaacttgat gaatcagttg tctatgggaa ccatatcgat gaattgcttc
    14521 gcgacttgaa caaagactaa tcaaaaaatc ccgcaaatcc tgtgaaatca ggggttgcgg
    14581 gatttttttg ttttcattaa gacgtattac ctaagagttc catgacttta gtctggtagc
    14641 gtgtcagcta accaaaaaag agaaaaagaa aagtaaaaaa ttcgactttc ttttttctat
    14701 agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt
    14761 aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac
    14821 cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat
    14881 tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt
    14941 agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt
    15001 tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccaacaat
    15061 gtgttaactg gcctagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc
    15121 ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag
    15181 gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg
    15241 aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg
    15301 aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg
    15361 gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata accccttact
    15421 gagacgtaca ctgaaaccag aagatgtcaa aaaacatcca atcggtcatt ggggaacgat
    15481 tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa
    15541 catgttttat atcgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt
    15601 agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa
    15661 attattcaag cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc
    15721 aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt
    15781 attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg
    15841 tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt
    15901 tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa
    15961 atcagatgga gaattagcaa attactttaa cggacttggc tgggagcctt tctttattga
    16021 aggaaatgat ccagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga
    16081 gaaaatcaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc
    16141 aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga
    16201 cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga
    16261 acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt
    16321 atttgatgca caaggacgta ttttagaaga aatcagagaa attgcgccta ctggtgacca
    16381 aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc
    16441 agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat
    16501 gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat
    16561 atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg
    16621 ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt
    16681 catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac
    16741 tggacggcat ggtttttttg caagttacga atcattcttg cgagtggttg actctatgtt
    16801 gactcagcac ttcaaatgga tacggaaatc acatgacctt tcttggcgaa aagattatcc
    16861 ttcgctgaat ctcattgcaa gctcaacagt cttccagcaa gatcacaatg gatatagcca
    16921 ccaagatcca ggaatattga cacatttggc agaaaagaag gcggagttca tccgtgaata
    16981 tctgcctgcg gatgcaaata cattgctcgc agtcatggat aaagcattcc gctcgtcaga
    17041 aaaaatcaat ttgatcatct cttccaaaca tccgcgtgct caattttact cagcagaaga
    17101 agcagccgtc ttagtgaatg aaggcttgaa aatcattgat tgggcttcga cagcaaaaga
    17161 ggaggaacct gaattggtca ttgctgctgc aggaacggaa tctaacttag aagcattagc
    17221 agctgtaact ttgctgctgg aagaattccc aaaactaaaa attcggttca tcaatgtcgt
    17281 agatttattg aaattacgtc atccaagtca agatccgcgt gggttgagcg atgaagaatt
    17341 tgatcaatat tttacaaaag acaaaccgat cttatttgct ttccacggtt atgaaacatt
    17401 ggtccgcacg attttctttg atcgtcataa ccatcattta atgattcatg gctacaaaga
    17461 aaatggagat atcacgacac catttgacat gcgtgtagta aatgaacttg atcgttatca
    17521 tttggcaaaa gacgcagctt taaaaatcaa aggaagtcaa gcagaagatt ttgcggaaaa
    17581 aatggatcag aaattacaag aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc
    17641 agaagtattg gattggaaat ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc
    17701 aaaacagaat tttttacatt gtattatttg tcaaattaag
//
LOCUS       DAZAJZ010000058        17099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000058 DAZAJZ010000000
VERSION     DAZAJZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.058"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            419..751
                     /locus_tag="ACG86D_001876"
     CDS             419..751
                     /locus_tag="ACG86D_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3141013.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            661..855
                     /locus_tag="ACG86D_001877"
     CDS             661..855
                     /locus_tag="ACG86D_001877"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3141014.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            894..1040
                     /locus_tag="ACG86D_001878"
     CDS             894..1040
                     /locus_tag="ACG86D_001878"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141015.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1478..2818
                     /locus_tag="ACG86D_001879"
                     /pseudo
     CDS             1478..2818
                     /locus_tag="ACG86D_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3059..4675)
                     /locus_tag="ACG86D_001880"
     CDS             complement(3059..4675)
                     /locus_tag="ACG86D_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN3141016.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4793..5011
                     /locus_tag="ACG86D_001881"
     CDS             4793..5011
                     /locus_tag="ACG86D_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141017.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5206..5667
                     /locus_tag="ACG86D_001882"
     CDS             5206..5667
                     /locus_tag="ACG86D_001882"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3141018.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5860..6099)
                     /locus_tag="ACG86D_001883"
     CDS             complement(5860..6099)
                     /locus_tag="ACG86D_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141019.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6123..7646)
                     /locus_tag="ACG86D_001884"
     CDS             complement(6123..7646)
                     /locus_tag="ACG86D_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN3141020.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7664..7786)
                     /locus_tag="ACG86D_001885"
     CDS             complement(7664..7786)
                     /locus_tag="ACG86D_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN3141021.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7816..8799)
                     /locus_tag="ACG86D_001886"
     CDS             complement(7816..8799)
                     /locus_tag="ACG86D_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN3141022.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8823..8972)
                     /gene="rpmG"
                     /locus_tag="ACG86D_001887"
     CDS             complement(8823..8972)
                     /gene="rpmG"
                     /locus_tag="ACG86D_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3141023.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8993..9370)
                     /locus_tag="ACG86D_001888"
     CDS             complement(8993..9370)
                     /locus_tag="ACG86D_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141024.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9402..9581)
                     /gene="rpmF"
                     /locus_tag="ACG86D_001889"
     CDS             complement(9402..9581)
                     /gene="rpmF"
                     /locus_tag="ACG86D_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN3141025.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9596..9865)
                     /gene="rpsN"
                     /locus_tag="ACG86D_001890"
     CDS             complement(9596..9865)
                     /gene="rpsN"
                     /locus_tag="ACG86D_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN3141026.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10388..12154)
                     /locus_tag="ACG86D_001891"
     CDS             complement(10388..12154)
                     /locus_tag="ACG86D_001891"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141027.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12114..13868)
                     /locus_tag="ACG86D_001892"
     CDS             complement(12114..13868)
                     /locus_tag="ACG86D_001892"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141028.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13978..14547)
                     /locus_tag="ACG86D_001893"
     CDS             complement(13978..14547)
                     /locus_tag="ACG86D_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN3141029.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(14565..15980)
                     /locus_tag="ACG86D_001894"
     CDS             complement(14565..15980)
                     /locus_tag="ACG86D_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141030.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(15991..16659)
                     /locus_tag="ACG86D_001895"
     CDS             complement(15991..16659)
                     /locus_tag="ACG86D_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN3141031.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(16789..>17099)
                     /locus_tag="ACG86D_001896"
     CDS             complement(16789..>17099)
                     /locus_tag="ACG86D_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3141032.1"
                     /translation="FENKRIFDLILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTI
                     GMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS
                     "
BASE COUNT         5529 a         3264 c         2537 g         5769 t
ORIGIN      
        1 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
       61 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
      121 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
      181 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
      241 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
      301 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
      361 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
      421 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
      481 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
      541 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
      601 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
      661 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
      721 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
      781 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
      841 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
      901 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
      961 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     1021 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     1081 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     1141 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     1201 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     1261 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     1321 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     1381 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     1441 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     1501 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     1561 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     1621 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     1681 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     1741 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     1801 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     1861 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     1921 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     1981 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     2041 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     2101 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     2161 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     2221 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     2281 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     2341 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     2401 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     2461 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     2521 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     2581 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     2641 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     2701 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     2761 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     2821 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     2881 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     2941 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     3001 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     3061 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     3121 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     3181 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     3241 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     3301 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     3361 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     3421 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     3481 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     3541 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     3601 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     3661 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     3721 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     3781 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     3841 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     3901 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
     3961 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
     4021 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
     4081 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
     4141 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
     4201 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
     4261 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
     4321 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
     4381 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
     4441 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
     4501 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
     4561 catttacaat gacagcaatt ttctttccgt gatcaccgta cagaatatga tttagaagta
     4621 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
     4681 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
     4741 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
     4801 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
     4861 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
     4921 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
     4981 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
     5041 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
     5101 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
     5161 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
     5221 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
     5281 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
     5341 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
     5401 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
     5461 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
     5521 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
     5581 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
     5641 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
     5701 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
     5761 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
     5821 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
     5881 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
     5941 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
     6001 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
     6061 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
     6121 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
     6181 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
     6241 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
     6301 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
     6361 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
     6421 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
     6481 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
     6541 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
     6601 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
     6661 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
     6721 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
     6781 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
     6841 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
     6901 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
     6961 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
     7021 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
     7081 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
     7141 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
     7201 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
     7261 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
     7321 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
     7381 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
     7441 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
     7501 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
     7561 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
     7621 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
     7681 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
     7741 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
     7801 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
     7861 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
     7921 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
     7981 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
     8041 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
     8101 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
     8161 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
     8221 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
     8281 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
     8341 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
     8401 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
     8461 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
     8521 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
     8581 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
     8641 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
     8701 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
     8761 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
     8821 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
     8881 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
     8941 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
     9001 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
     9061 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
     9121 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
     9181 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
     9241 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
     9301 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
     9361 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
     9421 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
     9481 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
     9541 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
     9601 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
     9661 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
     9721 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
     9781 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
     9841 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
     9901 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
     9961 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    10021 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    10081 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    10141 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    10201 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    10261 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    10321 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    10381 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    10441 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    10501 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    10561 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    10621 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    10681 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    10741 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    10801 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    10861 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    10921 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    10981 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    11041 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    11101 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    11161 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    11221 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    11281 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    11341 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    11401 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    11461 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    11521 attctatagt ttcttgaaat tcttccgagt tctctctcaa agtatcgtaa tatttagtat
    11581 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    11641 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    11701 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    11761 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    11821 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    11881 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    11941 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    12001 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    12061 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    12121 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    12181 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    12241 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    12301 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    12361 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    12421 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    12481 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    12541 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    12601 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    12661 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    12721 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    12781 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    12841 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    12901 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    12961 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    13021 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    13081 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    13141 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    13201 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    13261 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    13321 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    13381 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    13441 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    13501 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    13561 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    13621 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    13681 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    13741 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    13801 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    13861 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    13921 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    13981 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    14041 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    14101 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    14161 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    14221 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    14281 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    14341 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    14401 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    14461 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    14521 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    14581 ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt
    14641 ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga
    14701 gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc
    14761 atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac
    14821 aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat
    14881 tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta
    14941 actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc
    15001 atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct
    15061 ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt
    15121 ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata
    15181 ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga
    15241 acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt
    15301 aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt
    15361 aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt
    15421 aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt
    15481 tcatccaata taattaattt agagtttagc gataaactac atccaataga aattttctgc
    15541 cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat
    15601 tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc
    15661 gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt
    15721 acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga gttattttca
    15781 aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac
    15841 tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt
    15901 tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa
    15961 acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt
    16021 aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct
    16081 tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat
    16141 agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa
    16201 agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata
    16261 agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat
    16321 tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca accatgagat
    16381 cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa
    16441 aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa
    16501 gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat
    16561 gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat
    16621 atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa
    16681 gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct
    16741 tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa
    16801 ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc
    16861 ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa
    16921 gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt
    16981 atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa caatatcttg
    17041 atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat tctcaaaaa
//
LOCUS       DAZAJZ010000059        16958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000059 DAZAJZ010000000
VERSION     DAZAJZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.059"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG86D_001897"
     CDS             <1..1152
                     /locus_tag="ACG86D_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFN3141033.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG86D_001898"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG86D_001898"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN3141034.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG86D_001899"
     CDS             2728..3003
                     /locus_tag="ACG86D_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN3141035.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG86D_001900"
     CDS             3009..3776
                     /locus_tag="ACG86D_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN3141036.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG86D_001901"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG86D_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN3141037.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG86D_001902"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG86D_001902"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN3141038.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG86D_001903"
     CDS             7920..8222
                     /locus_tag="ACG86D_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN3141039.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG86D_001904"
     CDS             8225..9388
                     /locus_tag="ACG86D_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN3141040.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG86D_001905"
     CDS             9414..12842
                     /locus_tag="ACG86D_001905"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN3141041.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG86D_001906"
     CDS             12863..13987
                     /locus_tag="ACG86D_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN3141042.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG86D_001907"
     CDS             13968..14414
                     /locus_tag="ACG86D_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3141043.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG86D_001908"
     CDS             14398..14736
                     /locus_tag="ACG86D_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN3141044.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG86D_001909"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG86D_001909"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN3141045.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG86D_001910"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG86D_001910"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN3141046.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG86D_001911"
     CDS             15893..16936
                     /locus_tag="ACG86D_001911"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN3141047.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5746 a         2933 c         3744 g         4535 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggt
//
LOCUS       DAZAJZ010000060        16906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000060 DAZAJZ010000000
VERSION     DAZAJZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.060"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..587)
                     /locus_tag="ACG86D_001912"
     CDS             complement(75..587)
                     /locus_tag="ACG86D_001912"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN3141048.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(601..2898)
                     /gene="recJ"
                     /locus_tag="ACG86D_001913"
     CDS             complement(601..2898)
                     /gene="recJ"
                     /locus_tag="ACG86D_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN3141049.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3040..3483)
                     /locus_tag="ACG86D_001914"
     CDS             complement(3040..3483)
                     /locus_tag="ACG86D_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN3141050.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3484..4272)
                     /locus_tag="ACG86D_001915"
     CDS             complement(3484..4272)
                     /locus_tag="ACG86D_001915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3141051.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4269..5210)
                     /gene="rnz"
                     /locus_tag="ACG86D_001916"
     CDS             complement(4269..5210)
                     /gene="rnz"
                     /locus_tag="ACG86D_001916"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN3141052.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5387..5974)
                     /locus_tag="ACG86D_001917"
     CDS             complement(5387..5974)
                     /locus_tag="ACG86D_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN3141053.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6195..7508)
                     /gene="obgE"
                     /locus_tag="ACG86D_001918"
     CDS             complement(6195..7508)
                     /gene="obgE"
                     /locus_tag="ACG86D_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN3141054.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7796..8806
                     /gene="gap"
                     /locus_tag="ACG86D_001919"
     CDS             7796..8806
                     /gene="gap"
                     /locus_tag="ACG86D_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN3141055.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8891..9826)
                     /locus_tag="ACG86D_001920"
     CDS             complement(8891..9826)
                     /locus_tag="ACG86D_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3141056.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVAPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10012..10971
                     /locus_tag="ACG86D_001921"
     CDS             10012..10971
                     /locus_tag="ACG86D_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFN3141057.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11057..12151)
                     /locus_tag="ACG86D_001922"
     CDS             complement(11057..12151)
                     /locus_tag="ACG86D_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN3141058.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12340..13101
                     /locus_tag="ACG86D_001923"
     CDS             12340..13101
                     /locus_tag="ACG86D_001923"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3141059.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13216..13656)
                     /gene="fabZ"
                     /locus_tag="ACG86D_001924"
     CDS             complement(13216..13656)
                     /gene="fabZ"
                     /locus_tag="ACG86D_001924"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN3141060.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13876..14631
                     /gene="fabI"
                     /locus_tag="ACG86D_001925"
     CDS             13876..14631
                     /gene="fabI"
                     /locus_tag="ACG86D_001925"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN3141061.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14741..15793)
                     /locus_tag="ACG86D_001926"
     CDS             complement(14741..15793)
                     /locus_tag="ACG86D_001926"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN3141062.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            15919..16839
                     /locus_tag="ACG86D_001927"
     CDS             15919..16839
                     /locus_tag="ACG86D_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN3141063.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4918 a         3642 c         2965 g         5381 t
ORIGIN      
        1 agaatctgac ttttgagggt cgctttaaaa tcgcttcttt aagaataaca tcaagaatag
       61 cttctttttt ctgcttaata ttccattaat gtaatgatgt catatccttt gattttgtcg
      121 cgtccatgga gatccatcaa ttcgatcaaa aatgcgcagc ctacgactac accgcctaat
      181 tgttcgatca gttcgatcgt cgctttgatt gttcctccag tagctagcaa atcgtcacaa
      241 atcagcacac gttgacctgg tttaatggca tccttatgca atgtcaatgt atctgttcca
      301 tattctaatc cataagtcac ttcgatggtt tctcgaggta acttcccttt tttgcggact
      361 ggggcaaaac cgactcctaa ctcataagca acaggacatc ctacgatgaa tccacgggct
      421 tctggcccaa ctaccatatc aattcgtttt tcttttgcat aatttacgat ttgtttagta
      481 gcttcccgat aagcgtcacc atctgccatc agtggggaaa tatctcgaaa cacaattcct
      541 tttgacggat agtcgggaat actggcaata taatctttta aatccactct ttgtttcctc
      601 ctaggttgtt aaccatttct ttatggtagg aatatcgctc aaaagcaaaa attcctcaac
      661 tttgatttta ttgagccgtc gctggtataa tctgctctca gtcaaaggat gactttctgg
      721 gctagcgact ttttgtaaaa caccgttctc tattgtaaca aatttcaatt caaaaaacac
      781 ctgtatcata aaaattaata atttttcagg gatacgcaaa tattgagcta ttgcttttat
      841 tttatgacgt atatcaattc gttcttgaga atggatcaat ttaaacaatt ttgcgtactg
      901 ttctcttgtg cctaccccat tcaaataggc ctcatcaggt gaaatcccca tcagataaac
      961 acgattgaat gaataagacc gaaaaacttc tttcagatcc gctaaatcat ctggacaatc
     1021 cataacaact agagagtttg gttttctttt ttcaattatt gcctttacag aatctagtcc
     1081 ttcgaaaaca atcgcagctt cctggataat ctcaggcacg aatttcaacg tcgaagaatc
     1141 aaatgctaaa taaaggctac cttccgacgt tcggctctgt tcacggaaac gttttgcccg
     1201 ccaatcaaat acttgcagac cacccacaga aaaatcagat accatcagct gcggtttctt
     1261 acgcccgttc cattcattga tcgaaagctt tcctaccaca tctacctgat tattcagcag
     1321 ttcttcttct tgagtaccaa aaccaaaagc gaccgcatca agctgactcg atgcatcgga
     1381 aaggacaaat ttcagatgct gttggtttgc tccgattctt ttgatatttt ctgcttggac
     1441 ttgtcgaaat aaaaactgag gcaaaggatt atccgtacca aatggcgcca atagtttcag
     1501 ctcatcaatt cgctctactg ttacttcatt tggcagcaat acttcatcaa ttctcaactc
     1561 aggccctctc atcaagtcaa tttgatgatc gactatatat tggttcatct tctcctgtag
     1621 tatagtgaca ttttcacttg gcatcgttaa accgactgct gcatgatgtc ctccgaagta
     1681 agtaaataaa tctcgcatct ggtcaagcat ttcaaaaaga ttcaaggctt caatactacg
     1741 tccagatcct ttagctgatc catcttcttt taaagtaagg acaagcgttg gtttacccgt
     1801 ttcattcatt attttcccag caacaatgcc caaaacgcct tcatgccatc ctcggtttac
     1861 caatagatgg atatgattct ccttattgac catcgctaat gcttcttgtg ttatctgctc
     1921 tacgattgct tttctttctt cattgatctc attcaatttt tttgcttgaa cggatgcttc
     1981 ctcttcatca aacgtcgcaa gtaacgagac ggcaggacgt ggatcgccca tccgaccgat
     2041 tgcatttagt ctgggagcaa tcgaaaaacc gatcgttgtt tcgtctgcgt ccctcatttt
     2101 tacgccgctt tcttcaaata gcgcctgaag tcctatccgt tctgaatgat gaatggcatc
     2161 gattcctaat gaaacaagga ttcgattctc atcagtcaat gaaactaaat cggcgatcgt
     2221 tcctatcgct actaaatcca aaaattctgc aggcggttct tccaataatg cagaagctac
     2281 tttgaatgct actccgactc ctgccaaatc tggaaatgga tagtgccccg ctggatgacg
     2341 agggtgaaca atcgcataag cgtctggtaa attctcaggt agttcgtgat gatctgtaat
     2401 gatcacgtct acaccagctt gctgagcgta tgctaccgca tcatttcctg ctaccccgtt
     2461 atcaacagtg atgatcaact ggattccttc ttcgatcttt tcttggtata ccgattggtt
     2521 tggtccatac ccatgctcaa atcgatttgg caggaatact tctacttctg caccaagtaa
     2581 ttccagtgtt tctttcatta ccgttgcact ggtgatccca tctgcgtcat aatcaccgta
     2641 aacgaggatt ttttctccac tgattacagc ttcttggatt cggctgattg ctttatccat
     2701 atcgtgtatc agatacggat catgtaattc ttgttccttt ggatgtaaaa atcggttgat
     2761 atccttttcc tcatggtaac ctctttgcca cagtaactta ccgacaaatg gagataattt
     2821 cagttcacgg atcatttgga gaaaggattc agaaggctct gtctcgctta acttccaatc
     2881 aaaatttgct tgtctcactt tctcactcct aacttagtta ttatagcaaa ggaaatcagc
     2941 aaaaaaaagc agaaagaaag aataaaaaca gatcagatcc gctagacaga tgttcagcag
     3001 gacctgatct tttgtacgac catttatgaa atatccctat tagtcaaaat aattgaagct
     3061 ttgtttgggc gattgagttt tttgttcttc cggcgctttc aattgactga tttgtgcggt
     3121 tttcgcttgt aactccgcct gataagcagc ttccaactcc gcacgttcgt ttctaaactc
     3181 gcgttgctgt tcttcttttg cttcttcaat tttcttttga agttctgttt gatattctga
     3241 catttcctga gtcagctgtt tgatttgctt tttttgctgc catagtgttg ccgaagcagt
     3301 caaaaataca atcagtgcac caatcagcgc agaaccgatg atcactaaga tcaatggcgc
     3361 agaaaaggaa gtgaagccaa aacttacagg aacattttta ttgttcaata cagcaaaaag
     3421 aacaataatc aaaactaaaa caaatccaag aatcacattg ctttgctttt tcaattcctt
     3481 cacctacttt ttgttaaaaa ttccgctggc taaataatca ccaatcgttg gaaacagcgt
     3541 ataaaatttt gatgcaactt ctaaaataaa cggttgattg atttcccgtt ttggatgtct
     3601 catattgcgg acgatcattt ttgccaactt ggcaggttct aaaacgattt gtccgacttt
     3661 ttccaaatag cttccgcttg gatcagcctt atcgaagaaa tttgtctcga ttggacctgg
     3721 attcactgtc gtaacagcta cgccaagtgg tttcaattct aatcgcaaag cgttggagaa
     3781 gccaaggact gcaaatttcg tggcagaata aaccgttgat tttgcagttg ccatttttcc
     3841 agccatagag gcgatattaa tgatatgacc ttgtcttctt tctgccatat cgatcgcaaa
     3901 tttttgtgtc aaaaccatca ttcctaagat gttgacttca aacatatcgt atgcttttcc
     3961 aaggtcgaat gtaaggaagt cttcgaaaat cccaaaaccc gcattgttta ccagaatatc
     4021 gacgcgcccg acttcttcca tcaccttttc atataattcg tccacgcttt caggattgct
     4081 gacgtctacc ttgaaagcat aagcctcttt gccgctcaag cgtctgcact cgtctctgac
     4141 accttctaca aatgccattc ttcgtgcgca acttataacg attgctcctt gctttgctgc
     4201 ttcataacag atttgtgcgc ccagaccgcc tgaactacct gttactaaaa caactttatc
     4261 ttctaaattc atgcctcttc ctcctcgcga aatggaatct caatgctatc aaaatctttc
     4321 ataatcgtcg tgttttcaaa cacttcttgt gcttcttttt caagctctaa agctgctttt
     4381 cctaaatatc tcgcactgat atgagtaagt agcagccgtt ttgcctgcgc ttcttttgca
     4441 acttcagcag cttgatgagt cgtggaatgg aaatagttgt gcgccattct tgcttcatct
     4501 ttattaaatg tgctttcatg taccagaata tcgctatttt ccgcaagcac aacggaatta
     4561 tgtgtttttc tcgtatcgcc taaaatagtc acgattcgtc cttttttatc tggacctacg
     4621 aaatcttttc cattgatcgt ccgcccatct tctagttgga tcgtttcacc ttgcttcagt
     4681 ttgccgtaca gtggtccagc tggaatacct aattctttta gacgatccac ctgcaattct
     4741 cctttatgat catgttcaac cacacgataa ccaaaactgt cgatcccatg atttagtttt
     4801 tttgtataaa cagaaaattg ctgatcagta aaaatcgggg ctgtttcatt tagctcaatg
     4861 aatttcagcg gataacttaa acgcgtttgc gaaataccta aagagacttt tatgtattcc
     4921 tctatccctt ttggcccgta aatctctaat ggcgtatccc caccttgaaa tgaccggctg
     4981 ctgatgagcc cgggaagtcc aaaaatatga tcgccgtgta aatgtgtaat gaaaatttta
     5041 ccgatttttc tcggacgaat agtcgttcgc aaaatctgca tttgcgtgcc ttcgccacag
     5101 tcgaacaacc aaacttcatt gcgctcgtct aaaagtttca atgctatact tgtcacattt
     5161 ctatgttttg caggaacacc tgcacctgtt cctaaaaatt gtaattccat aatctacctg
     5221 actttcttta agttcctctt taattttagg gtaaaaagaa gggataatca accgtaagtc
     5281 gagaatgggt tcaaattaaa aataacgttc taaagcgata gcgttttctt tgaaaattac
     5341 tataaaaaca gcagcgaacc cgtttgagat cctctgctgt tttttattaa tttgaagatc
     5401 cagaactttc attgtcgttg gaagtttgcg gatgtttgtc cttccaagtc ttttgtgcag
     5461 cttctttcgc atgattttca gcttctttcg catcatgcac atctttttcg acatcttcat
     5521 aatccaagcg agtaatttct ccgcctaatt ccatcattac caattgattc agtgggcgat
     5581 tgtcctcttc atcagcaatc aagatcagtc ctgtagctcc ttcaccgatt ttatttccta
     5641 caaagtcgaa cacggtctgg gcatctttta tttctttggc atcttgtgtc gcaccgatca
     5701 ttcctccggc aaaccatcct aataaaatcc ctagcggacc acctaataca ccaaccaaca
     5761 ttccgattaa tccacccgta gaagatttgt tattaccggt aaagtcgata aaatcttcta
     5821 ttttgaattg atgtgtccca ttaggatcat ggctgacaac tgccatctgt tctcctttaa
     5881 tcgaacgaac agcatgtaac tttttgattt ctgaaaatgc ctgatatgcc tggctttcta
     5941 cttgaaaatt taaaatgatt actcgtttgc tcattaaaaa cgcccctttc ataccttctt
     6001 gttatttatt ttacctttaa ttgaaaaata agaaaagaaa agcactcttt ttttccaata
     6061 aaatagggct gtgacaaaag tcgtatcaca gctccttttt ccgaataaac ggcgttcttg
     6121 aagctgatca cttctgtcac aacctccctt aaatctgaat ctatattttt tggtatttgt
     6181 ttttatttgc ttgcctattc gacaaattca aattcaaact caccgatacg tacaaggtcg
     6241 ccatctttcg caccgcgtgc ccgaagagct tcatccacac ccataccacg taattgacga
     6301 gcaaaacgca tcactgtttc atcatgatcg aaattcgtca tttggaataa tttttcgatc
     6361 ttctcaccag ataagatcca cgttgcatct gaatcacgat cgatttggaa ctcaggacct
     6421 tctggattga atccatagtg tacagtttct tcttccagtt cttcttcgta caatgggaat
     6481 tctggcgtta cttctaacaa atctgctgta gcattcaata acgcgtccaa tccttgtcga
     6541 gtcactccag aaattggaaa tactgggatg tcatcagcaa attcatcttc tttttccttg
     6601 ttcagctgtt ctttgaattt cactagattt tccggtgcat ccggcatatc cattttgttt
     6661 gccacgatga tttgcggacg ctctagcaag cgtaagttat aagtagataa ttctttgttg
     6721 atggcaagat agtcttcata aggatctcgt ccttccatcc cactcatatc gatcacatgt
     6781 aaaattactc gtgttctttc gatatgacga aggaattgtg tacccagccc aacaccttgc
     6841 gaagctcctt caatcaagcc tggcaagtct gctgcggcaa aacttctgcc atcacttgta
     6901 gtaaccattc ctagattagg tacgagcgtc gtgaaatgat aagcgccgat ttttggacga
     6961 gcagatgaaa taacggatag taaagtcgat ttcccgacag atggaaaacc tacaagtcca
     7021 acgtctgcca atactttcaa ttctagttcg atttttcgct cttgtcctgg ttcaccgttt
     7081 tcagcaattt ccggtgcagg attccgagga gaggcaaaac ggatattccc tcgtccaccg
     7141 cgtccacctt ttgccacaac tagtgtttga ccattttcaa ttaaatctcc taaaagagca
     7201 ccagtctccg catctcggac agtggttcct tgtggtactt taacataggt atcttctgaa
     7261 ccacgaccgt gcatcccttt actcatccca ttttcacctg gctgcgcttt gaaatggcga
     7321 ttgaaccgaa aatccatcaa tgtacgtaac ccttcatcga cgatcaagat gacatcgccg
     7381 ccacgaccac cgtctccgcc agccggtccg ccgtcaggaa cgtatttttc gcgacgaaat
     7441 gcaaccattc cgtctccgcc cttgccagct ttcacatcga tcgttacttg atctaaaaac
     7501 atggacataa ttaagtcctc ctattattca tttatttatt tgactgcctg aatttccaca
     7561 ataaacaaaa gtcttactta ttttatcggt gaaatctgat tttgtctatc atttcagcaa
     7621 aaagattgtt taccttcttc tttttttatc atttggtaag gtttccaacc ttttttctgc
     7681 taccaagtat aattttttga caaaatattc tttgcgtagc agaataacag tgtattaatt
     7741 gtattaattg aggatgccgt ttcacagcac ccctaataaa ggaggaactt ttgatatgac
     7801 agtaaaagta ggtattaacg gatttgggcg tattggccgt ctggctttcc gccgcatcaa
     7861 agaagtctca gatgatattg aagtagtagc aatcaacgac ttaacaagtc caactatgtt
     7921 agcacaatta ttgcaatttg actctactca tggtacttat cctggcacag ttactgcaac
     7981 agaagactca atcgtagtgg atggcgaagc aactcgtgtt tatgcagaac ccgacgcaag
     8041 caaaatccct tgggcaaaag aaaacggtgt agatatcgta ctagaatgta ctggttttta
     8101 tacatctgaa gaaaaagcaa aagcgcattt agatgctggt gtcaaacgag tagttatttc
     8161 tgccccagct ggtgctatga aaaccatcgt ctataacgtc aatgatgata cattagatga
     8221 aaacgacaaa atcatctcag caggttcatg tacaacaaac tgtttagcac ctatggctta
     8281 tttcttaaat aatgaatttg ggatcgaagt tggtacaatg acaacagttc atgcctacac
     8341 ttccacacaa atgctcttag atggacctgt aaaaggcggt aatttgcgtg ctgctcgttc
     8401 agctgctgac aatactatcc cacattctac tggtgctgcg aaagcaatcg gcttagttat
     8461 ccctgagcta caaggaaaat tacaaggaca cgcacaacgt gttccagtcg ttgatggttc
     8521 attaactgaa ttggtttcag tattgaaaac aaaagtaact gctgatcaag taaacgaagc
     8581 aatcaaaaaa cacacagttg acaatccttc attcgggtat gatgaccgtc aaatcgtttc
     8641 tagcgatgtc attggaacaa ctcaaggttc gatctttgac ccaacgcaaa cagaagtaac
     8701 aactgctggt gatttccaac ttgttaaaac tgttgcatgg tatgacaatg agtatggatt
     8761 tacttgccaa atgattcgct tactagaaaa attcgctaac ttgtaatttg atagatcaat
     8821 cgataaaaga agcctttgac tggatccagt caaaggcttc tttcttttca tcccctactt
     8881 ttaaactcgt tcaatgaaca tattggctta aaaattgttg gactacttgc tgatattttt
     8941 caggatctgt cttgtatgct tccgcatgct cggctccttt tacaacatac ttttcttttg
     9001 gtcccttcgt tgcacgatag accttatcta acatttcaaa tggtacaaat gtatcttcgt
     9061 ctccatgtat aaagagcatt gggcgctggt ttttcttcaa ttgttccact gcatttgctt
     9121 caccgaaaaa gtagccagca cggacttttg tcactagact cgttacttgc ataagcggaa
     9181 atgctggaag actaaacata tcgtctagct gataagcaag ctcatcatga acggaagaat
     9241 aaccgcaatc ttcaatgatt gctttcacat tatctggtag tttttctccg ctagtcatca
     9301 taactgttgc tgctcccatg ctgacaccgt atagaacgat ttcttgcgat tttccattgt
     9361 tttctagcac tttgtttatc cactgtacat agtctttacg ctctggccat ccaaatccaa
     9421 tgtaatcccc ttcgcttttt ccatgtcctc ttgcatcagg aacaaggaca ttgtaaccca
     9481 tatcatgaaa catctttgcg taattggtca tcgtttcagc gtttcccata tagccatgag
     9541 ccacaagagc tgttttttca cttttcgttt cagcaggaag ataaatcgct gataatttca
     9601 gaccgtcttt tgaagtgatg gtccaatcgc ttcggttttc ttttgaacta aaccatttat
     9661 gtgcatccgt ttctttggat gtctgtgttg taccaggtgt atcttcgtct aaaaagtctt
     9721 tctcagaagg cgcaacagca taattataaa aataattccc tgcaaacact aacgcaacaa
     9781 tcagagctaa agcgacgcct aaaaggattt tcatccaaag tttcataaat catccatctc
     9841 atttctcttt tttcatataa ccttgaatta gtataatcaa agaatctcta ctatgcaatg
     9901 ttcattttta taacccgcga attgtaaaat taagttagaa aaataaaaag gcatttatgg
     9961 tacactcaaa ttgtatttcc gacgaaagaa aacaaaggag tgtaaccata aatgacctac
    10021 aaacatctta ccatagacga actgacaatg atagaatcat attatcttca acataataag
    10081 ccggttgaaa tcgctaaccg aatgggacgt gctatacaaa ctatttataa tgtagtcaat
    10141 aagttcaagc aaggcaagac tgctcttgat tattggcacc agtataaaga aaataagaaa
    10201 aaatgtggta gaaaagtcat tcaattacct gctcatgaag tagattacat taaagagaaa
    10261 gtcactcttg gttggacgcc tgacgtcatt atcgggcgaa aagaaaggtc tgtttcatgc
    10321 ggtatgagaa cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg
    10381 atgaaaggta aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag
    10441 cgctcaatcc atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt
    10501 gaaggtgata cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga
    10561 ttatctaaag tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact
    10621 gcccttaatc aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac
    10681 tgtggaaaag aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat
    10741 tttgcggacc ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg
    10801 cgtcgtaatg gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc
    10861 agtgtcagca atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc
    10921 gaaatatttt tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata
    10981 acctataaaa aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc
    11041 tgcaataact aattatctat tttttatttt caattaattt aaagcttgac catggtttca
    11101 aaaagtcaag gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt
    11161 cgtttttcat atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat
    11221 tgtcaatcag tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat
    11281 ctttgtagta tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat
    11341 aactatgaag gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat
    11401 ctactttcaa tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt
    11461 cacttgcgta cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct
    11521 tcacttgtgt cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg
    11581 taggccaagg gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat
    11641 attttctgaa tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat
    11701 gaggataaaa attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt
    11761 tgtcaacgta attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc
    11821 gcgtcatttt tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac
    11881 ccatcttatg gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg
    11941 gattgatatc aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa
    12001 attctgaaag aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt
    12061 tatacccata cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt
    12121 ctggataaat cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt
    12181 tttgtatcca cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct
    12241 tttaaaataa atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc
    12301 attaaagata acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt
    12361 ttaagtatta gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg
    12421 acttcttcta gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact
    12481 gccagtagcg tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa
    12541 aatgaagatc tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc
    12601 gaacacgcac caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac
    12661 ggactgactt tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc
    12721 gatggcactg gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac
    12781 ctttataata taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc
    12841 agccagttct ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac
    12901 tatcctcaag caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat
    12961 tatactgtct tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc
    13021 gaaacagata gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg
    13081 caagaagcaa aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg
    13141 ctaggattca ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta
    13201 tatcaggttt tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac
    13261 ttttttatca tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt
    13321 gatttcaaaa tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt
    13381 attgatccca ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat
    13441 cgaacctgct tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg
    13501 aaagtgacct tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt
    13561 tcctggttct aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa
    13621 ttccattact tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa
    13681 ttgccatttt cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt
    13741 ttttaattta agaaaaaata gtttgacaat caaaacattt tgactaataa ttaagaaatc
    13801 aagaagttat tttagctatt attctctatt tttttacgac tatcaaattt ttatcagaaa
    13861 caaaggagag tcattatgtc atttctagaa ggaaaaaaaa tcgtcattat gggcgtcgct
    13921 aacaaaaaaa gcattgcttg gggttgtgct gaagcaatgt tgaaacaagg ggcagaaatt
    13981 atctatactt accaaaatga acgaatgaaa aaatcattag tcaaattggt cggcgaagaa
    14041 gcattcactg tagaatgtga tgtttctagc gacgaaagca tcgaaaaagc aatgaatgcc
    14101 atcggtgaat atgctgggga gatccacggc cttgtccatg cagttgctta cgccaacaaa
    14161 gaagaattgt ccggaaatgt gtctgatatt tccagagacg gctatcaatt agctcaagat
    14221 atcagcagct actctttaat tgctgtatca aaatacgcac aaccgtttct tgcaccaaac
    14281 agcgggatcg ttagcatgac ttatctaggt tcagaacgcg caatcccgaa ttataacatg
    14341 atgggtattg cgaaagcttc tcttgaagct gctatccgtt atttggcagc tgagttctcg
    14401 cctaaaggaa ttcgggtcaa tggaatctca gctggggcaa tcaaaacatt agctgtcaca
    14461 ggtgttaaag actatcaaaa actgatccag ttatctgaat ctcgtacgcc tgatcagcaa
    14521 ggtgtgacga tcgaagaagt cggtaatgct tgtgctttct taatcagccc actttccagc
    14581 ggaatcatcg gtgatattat ctacgttgac aaaggtgtcc atctttcata aacttttgtt
    14641 gcattcttta aagtaaaaca tataaaaatc aggaaaaaac tttttaaagt ctcttcctga
    14701 ttttttcttt ttacgttttt ttcaactcat tactttgaaa ttattccacg acttcaatcc
    14761 agccttctgg tgcttctatg tctccaaatt gaatacctgt caattcatca tacaaccgtt
    14821 ttgttactgg tcctacttcc gtttcactat aaaatacatg aagattgtta ttgtgataaa
    14881 tcccaccgat tggagaaata acagccgctg taccacatgc accagcttct gcaaattgat
    14941 ctagatcatc aatgaaaata tctccttcaa ttgcttgcag tcctagtcgt tcttcagcta
    15001 actgcaacaa ggaatatttc gtgatacttg gtaagataga gggcgattgc ggagtgatga
    15061 attttccgtc ttttgtaatt ccaaagaaat ttgctgcacc tacttcttcg attttcgtat
    15121 gtgtggctgg atcaagataa acacaatctg agtaatccag ttcttttgct tctgttcctg
    15181 gaagtaaact cgctgcgtaa tttccgccga cttttgctgc accagtcccc ttaggagcag
    15241 cacgatcgta atcagataca ataaaattcg ttggttttag tccacctttg aaatacgctc
    15301 ctactggcgt acagaagacg acaaataaat attctggagc tggatggaca ccaatgtttt
    15361 caccgacccc aatcagtaaa gggcgcaagt ataatgtagc gcctgttcca tagggcggca
    15421 caaaatcttc atttgctttt accacttgct tgacagcatc aacgaacatc ttttcaggga
    15481 ccattggcat cagcaatcgt ctagcacttt gattcatccg tcgactattt tgttcaggtc
    15541 gaaagagatt gaccttgccg tcttttcgac gataagcttt tagaccttca aaacattgct
    15601 gaccataatg aagcgctgga gagccttcat gaatcgtcag gtaattatct tctgtcaatt
    15661 ctccttcttc ccattgtccg tctttccatt tggcaataaa acgccaagga gttttgatat
    15721 aagaaaaacc tagattgttc caatcgattt gcatatactt accaccttcg ttattttctc
    15781 cacattttat cattatccat attatttgtc attttatttt cagctttttt ccttcattat
    15841 agttattttt gctatttcct tttttgaaaa cttcttggta caatacagaa agaatcaaat
    15901 tttagaaaag aggattttat gttttcatta ttagcaactg ccggtacgac agactcttct
    15961 ggcgagacat tgacagatca agctgtcagc aaagtaaatg cgtttcagcg gttttgggac
    16021 gggattgatt gggacgccat tttggctgcc tttatccaaa aaagtttata tctgattttt
    16081 ttgatcgtcc ttttttggat tcttcaccgt gtgggaaaat atctgattga taagtctttt
    16141 aatcagtatt ctaaaaaagc cattctaaac gagagccgct taaagacttt acatagtctt
    16201 ttaaatacta tttttcatta tacccttggt ttctttttta tctatgcaat tttatctgct
    16261 atcggtgtgc cgatcggatc acttttggct ggtgccggaa ttgcaggtgt ggcaatcggt
    16321 cttggtgccc aagggttcat gagtgatgtt attaccggat tttttattat catggaacaa
    16381 cagatggatg ttggggacta catcaagcta gcaaacttaa cgattgaagg aaccgtcgta
    16441 tctgttggta tccgtaccct tcagctgaaa gcaacggatg gtactgtcca tttcatcccg
    16501 aatcgaaaca tcaccacgat cagcaatctc tctcgtgcaa atatgcaagt acttttagat
    16561 attcggattg ttcctgaaga aggatatgac aaaatctatg agatcattga tcgagtaaat
    16621 cgtacattag ctgaaaaata ccaagaagag ctccaaactg aaccttctat ctttggatta
    16681 gtggatcttg gaaacagcaa ttttgcgatt cgtacggtct gctacgcatt gaacggaaaa
    16741 caatatgcct taaaagaaga gttcttgagc tcctatgtca aagaattgac agcttctggt
    16801 ttcacgattc cgaacagtcc aattgctgtc ggaaaataat ttggacaaac gatgcaacga
    16861 atctcactaa aaagagcgtg ggacaaaagt aaagaacact tttgtc
//
LOCUS       DAZAJZ010000061        16797 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000061 DAZAJZ010000000
VERSION     DAZAJZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.061"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..331)
                     /locus_tag="ACG86D_001928"
     CDS             complement(20..331)
                     /locus_tag="ACG86D_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3141064.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(333..530)
                     /locus_tag="ACG86D_001929"
     CDS             complement(333..530)
                     /locus_tag="ACG86D_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN3141065.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(924..2651)
                     /locus_tag="ACG86D_001930"
     CDS             complement(924..2651)
                     /locus_tag="ACG86D_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN3141066.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2644..3837)
                     /locus_tag="ACG86D_001931"
     CDS             complement(2644..3837)
                     /locus_tag="ACG86D_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3141067.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4024..4668)
                     /locus_tag="ACG86D_001932"
     CDS             complement(4024..4668)
                     /locus_tag="ACG86D_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3141068.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4762..4896)
                     /locus_tag="ACG86D_001933"
     CDS             complement(4762..4896)
                     /locus_tag="ACG86D_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN3141069.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4898..7030)
                     /gene="feoB"
                     /locus_tag="ACG86D_001934"
     CDS             complement(4898..7030)
                     /gene="feoB"
                     /locus_tag="ACG86D_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN3141070.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7027..7500)
                     /locus_tag="ACG86D_001935"
     CDS             complement(7027..7500)
                     /locus_tag="ACG86D_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN3141071.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7901..8125
                     /gene="nrdH"
                     /locus_tag="ACG86D_001936"
     CDS             7901..8125
                     /gene="nrdH"
                     /locus_tag="ACG86D_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN3141072.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8284..10443
                     /gene="nrdE"
                     /locus_tag="ACG86D_001937"
     CDS             8284..10443
                     /gene="nrdE"
                     /locus_tag="ACG86D_001937"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN3141073.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10493..11458
                     /gene="nrdF"
                     /locus_tag="ACG86D_001938"
     CDS             10493..11458
                     /gene="nrdF"
                     /locus_tag="ACG86D_001938"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN3141074.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11547..12686)
                     /locus_tag="ACG86D_001939"
     CDS             complement(11547..12686)
                     /locus_tag="ACG86D_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3141075.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12995..13708)
                     /locus_tag="ACG86D_001940"
     CDS             complement(12995..13708)
                     /locus_tag="ACG86D_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN3141076.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13962..14663)
                     /gene="nagB"
                     /locus_tag="ACG86D_001941"
     CDS             complement(13962..14663)
                     /gene="nagB"
                     /locus_tag="ACG86D_001941"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN3141077.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14897..16429
                     /locus_tag="ACG86D_001942"
     CDS             14897..16429
                     /locus_tag="ACG86D_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3141078.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16620..>16797)
                     /locus_tag="ACG86D_001943"
     CDS             complement(16620..>16797)
                     /locus_tag="ACG86D_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3141079.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5218 a         3453 c         2889 g         5237 t
ORIGIN      
        1 tttaatatta taagtatcat taaaattgag atttatctcc ataatctgct cgatataaac
       61 catttattcc agcagttctt ccaatttcac tcaaatttct tattaattcc tcttgatatt
      121 cagtgaatct tattttatta gtataagcag taaaatatag ataattcacc aatctattta
      181 ttaaaggact gggatctaat tcatattttt gtttttctaa ttttaaatat accttttgta
      241 aaacctcaag aatatcagaa tatttaggat ctttacttat agatatatca ttatataatt
      301 catgaacaat ttgcttagca ttttttttca ttttagcact tccctggaat tgctccacct
      361 aaaaaaccac ctatagacat tcctgcaata caagtagttg ccttggccca atcgaccgta
      421 catttatttt tagtgcaata cactccattt ccataatatt ttccactatg agtggtaccc
      481 ccatagataa gttgtgtctc tttaatagac aaaattttta aatgtttcat aatctaatca
      541 cccctaaatt aatgttttta gactattatt ctaacattca ttaaatttca aaaaacaatt
      601 ccttcttgaa acgtagcaaa ataattcctg aataagaatt taatcacttt taagtatata
      661 aactatgtat attctttaat attttttgag aatgcacttc gtacataata aactttaatc
      721 acattagtct ttcataccta cgatcgatct ctttgactcg cacccataaa ttcataatag
      781 tttgtcggcg tctttgaacg tttcgaagat gagctacgca ttctcttttt ttatagacaa
      841 aaaagataga gacgaacatg gagcttccaa atttggatat ttgatccaat tttggaagct
      901 ccatttattt cgtgtctgtc tttttatttt ttccagcttt cgactaattc ttggttctct
      961 ttgatccagt cttttgctgc ttgctgtgga tcttttccat tgttgatttc caacataact
     1021 ttttccatat ctttttctga ccaatggaaa ttgtctaata ctttgtaagc ttctggctgg
     1081 tcttctttca atccttttcg aaccatcgta tggatctgct cttcgcttcc cattccgttt
     1141 tcaggatctt ctaagtattt cagatcatat ttggcaaaca tccaatgagg tgtccatcct
     1201 gtaactacaa ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt cattgctcct
     1261 gaagaagatg tctctacttt ccagtctttc aagttgtcat atttttggat cgtgttttca
     1321 gcagccgtaa ctacaccagc tcctggctcg attcctatga ttttcttacc tgcctgatcg
     1381 gttaactcat cgatcgaatt tacatccatg taggctggca ctgctaaacc aacttttgct
     1441 cctgtcaaat tggcacctag gtcttcgact tgatctttat attgtgcata ttgtgaacca
     1501 tgagttttag gtagccaagc tgaaaccatg gcatctgact cgccatttga aacagatttc
     1561 cacatgattg aattatctaa tggcgtcatc gttacatcat agcccatttg tttcaatact
     1621 tcaccgacta catttgttga agcaacttct gtatcccatt caacataaga taagttgata
     1681 cgttttgttt ctttggcaga tgaaaaactc cctataccaa tcgcacctaa gatcactact
     1741 actacagcac caatgatcag tccttgacgt tttttacttt tctgtgttgt tgctcctgca
     1801 gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat gattgctaag
     1861 ataactaatg caaccccgtt gacaaatcca ttcccgactt gtgctctttg caatgctgat
     1921 aatacgccgc gtcctaagcc gggtgcaccg atcatagagg caataacgac cattgaaagt
     1981 gacagcattg ttgtttgatt gatccctgcc atgatcgtac ttttagctaa aggcaattct
     2041 agtttgaata gtttttgacg acttgtgctc ccgaatgaat cagaagcttc tactaattct
     2101 ttaggaactt gtcgaatccc taaattcgta aaacgaacgg ttggtggcag agcaaagatg
     2161 actgaggcaa atacccctgg aaccatcccg ataccgaaaa atgcaactgc tggaatcaaa
     2221 taaacgaatc ctggcattgt ctgcataaag tcaaggatcg gtttgataat actttgggct
     2281 ttactgcttt ttgccatcaa gatccctaat ggtaccccaa tgatgatcga gatcacacta
     2341 gataaaagaa caagtgtgac agtattcatc aaatcattcc atagaccttg gttataaata
     2401 aaccatagac cgattaatgt gaacaacgta agtcctggac gtttcttaga aataaagaaa
     2461 gctgctaccg tcaataacaa gataaacaaa ggagctggaa tcactaacag taaatttgta
     2521 atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc agaaaaagtt
     2581 gtcgtaaacc attctgtgat attttctacc cagtttgcca ctggtaattg atagttcaat
     2641 agattattca tgttcactca cctcgcttgt ttctgctaat gcttcaatca cactacctcg
     2701 gatgacgaca ccttttaatt ttccatcctc tacaactgca agtggagtcg gtgagtcata
     2761 aataatattg aaaatatcac ttaccaccat gtctttccca atcaccgtca cattttcatc
     2821 taggaagtcg atcaatggac gtttttgctt cctagcttct aacgcttgat ctgcagtgat
     2881 gatccctttc aggtgtctct ttctgtcaac agccatcagc atgctgactt cttcattacg
     2941 catccttgtc aatgcaacgt ttggtccatc agattcgata ttcgtcgtca atgcaggtac
     3001 catgatgttt tgagccgtaa gcactttgga acgatcgact tcttccacaa attcacgtac
     3061 ataatcattt gctggattgg tcaatatttc ttcacctgtt ccgatctgca tgatttcgcc
     3121 gtctttcatc aaggcgattc tgtccccaat ccgtaaagct tcattcaaat catgggtaat
     3181 gaaaataatc gttttttgaa cattggcttg caggtccagt aattcgtctt gcatttctcg
     3241 ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc agcaagatct caggatcatt
     3301 tgctaaagct ctggctaggc caacccgttg ttgcatccca ccagataact gactaggata
     3361 ttggtcttta aatgaaagca aactagaatt ttctaatgct ttttctgctt tttcttgtcg
     3421 ttcttcttta ggtacaccac ggacttctaa cccgtattct gtattttcca agatcgttcg
     3481 atgtggaaac agaccgaagt tttggaaaac catattgatt ttatgacgac ggacttctcg
     3541 caattcttct ttgcttaact tcgatacatc ttctccatca atatagatac tgcccgaagt
     3601 tggctcaatc agacgattca atagacggat caatgtggat ttcccactcc cggaaagccc
     3661 catgatcacg aagatttctc cttcttttac gtcaaaattc acgtcataca caccaaccgt
     3721 tgcaccggtt ttctttaaaa tctccgtttt cggctggtga tcattcatca tatctaaagc
     3781 ggcttgtgtc tttttaccaa atatctttgt taaatgtttt acttctactt ttaccaaaca
     3841 aattctcctt ttttatgtaa attcattttt ctttctgaaa aaagtgacca cactatgtta
     3901 acatcatgta gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac tgaaaaaaac
     3961 ttggctaaat ctttgtaaat aaaaggttta gccaagttaa tattttttta atatttcaca
     4021 caatcattct gcaagctgat gctccgggta aaaaaagttt gtcatacgct gtagtgtata
     4081 ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg ggccaggtga
     4141 aacaagcagt tcttcattat gagaaattgc gataactgtg gcgccagtcg cctgccagat
     4201 attcaactcg ctaattgaac ggtccagatg cattgctttt tctgataaag ataattcaaa
     4261 cggaagtaaa ggattcatct tatgtattcg cttcgtttga ttgaccagct gatccaataa
     4321 ttgagaaaaa tgattcagct catcttgttg cttctccaca cttgccatga tttctgcttt
     4381 tgtatcttgg atggattgta catcttgata ttgcaatagg aaatcttttg ctttttcctt
     4441 cgaagcaaca aatgccccac ttccatgccg tacttccatg attccaagat ctaccagcac
     4501 attgatagcc ttgcgggtag tctcaggaga agcattgtac gtactggcaa gtgttgagcg
     4561 ggcatggatc ttctcaccca ctttatatcg gttttcaacg atacgttcgg ctaaatcaac
     4621 agcaatctgc tggtagcgcg ggcggacgat ttttttcttt ccagacattt tctttcctct
     4681 ttcttttttt actttccctc atcttactat gctctagcaa aataagcaaa aaaggggaga
     4741 atagcctctt gcaacgactg atcatcgctt gatctgttcc ttttttactg ggcaagctgt
     4801 ttgacagtca tcacaattct taccggattt taaacgagta tagacaattg cgcctgcact
     4861 tccaaagatt aacacactta ataagaatgt cgccatccta ttcctctcct tctaatgaca
     4921 atatagtata aaactggttc ttggattttg gctgacggaa caaaacataa acgagtccta
     4981 tcatcagtaa aatagcaaga tacgtcccta acgctacctg tccaccctcc aataagtgtc
     5041 ccaactgata aatcacaaaa ctaactaaat aagctaaacc gcattgatag cctatggcga
     5101 tccaagtcca tttcttggca ttcatttccc gatggatagc cccaattgcc gcaaaacaag
     5161 gcgcacaaag taaattgaag gctaacaatg aataagctgc tgcgggagta tatgtgtgtt
     5221 gtagcacttg ccagatttct ctgccatttt cggatacttc cgcatgacca aacaaaatac
     5281 caaatgtgcc aataatattt tctttagcga ccaatcctgt aatcgctgcg actgttcctt
     5341 gccagtttcc ccaaccaagc ggctgaaaaa caactgccag gattcgcccg aaaaaggcta
     5401 agatactttg gtcttcagaa acaggttgag cataaaaatt ataagaagag ctgaaccaga
     5461 tcacgatact agaaacaaag ataatcgttc ctgctttttt aacaaaagac ttgctgcggt
     5521 cataggtttg tcttaggacc gctgaccatt gaggaaaatg atatgcgggc aattccatga
     5581 taaaaggagc tgtatctcca gcaaacaaac gtgttttttt caagctgatt ccagagaaaa
     5641 taattgcagt cataccaaga aaataggcag aaggagctac ccaagattga tgaggaaaaa
     5701 aggcccctgc tactaaggca ataatcggca actttgcaga acaaggcatg aaggttgtga
     5761 ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt gcgactggcc atgacgcctg
     5821 gcactccaca tcctgtagca atcagcatag gaatgaagga ttttcctgaa agaccgaatt
     5881 tcctgaaaag tcgatccatg acaaaagcga tcctcgccat atatccgcag tcttccagaa
     5941 agctcaagca taaaaacaat ataatcaatt gcggtagaaa tcctaacact gctcccacgc
     6001 ctgcgatgat gccgttcaag atcagctcct gcatccatcc cgctacttgc cattcagcta
     6061 acaatctgcc aacatgttct ggtaaccatg taccaaacaa tgtgtcattg atccaatctg
     6121 tccccattgt tcctactgtt tgaatagcca gataatagat cagccacatc acaaatgcga
     6181 agatcggcaa ggctaaccaa cgattcgttg caatctgatc gattttgtca ctcatcgata
     6241 attggaaaga atcatttgac acaacacaga gtgtacatag ttgggtaata aattcataac
     6301 gttcattgac cagaatcgtc tcactatcgt cgtccaatag tttctctgtt aactggatca
     6361 gttgctcgat ctctttttgc tgagaaactg ctatctcgta atcctctctt accttctgat
     6421 ctcgttcaaa atatttcatt gcataccagc gtttcttgcc gctttgttct cctagtacat
     6481 cttcaatttc atgtaaagct gcttctaaac gtggatcata tgtacaggga acaggaatag
     6541 aaggccgctt ttttgcagac tcctgaatct tttcgattag tttttcgatt ccttgctttt
     6601 tcaatacgct gattggtaaa actggtacac caagaccata ggaaagcttt tctagattga
     6661 ttttttttcc ttcttttttg atgacatcca tcatattcag accaatgatc ataggaatgt
     6721 ctaaatccaa taactgcgta gttaaataaa gatttcgttc taaattggta gcatcaataa
     6781 tgttgatgat cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc acttcttctg
     6841 gtgtataagg agacaaagaa tagatgccgg gtaggtcctg aatatgaatg ttcggatttt
     6901 ttttcaacga tccttctttt ttttctactg ttacgcccgg ccaattccct acatattgat
     6961 tcgaaccagt caagagattg aacaagctag ttttcccgct gtttggattt ccgactaacg
     7021 cgatattcat tctttttcct ccctttgtat caagattagt tcggcctctg actttcgtaa
     7081 cgtcagttca taccctcgca ctgtcagttc gattggatct cccagagggg ctagttttcg
     7141 gataactatt tttacatttc gcgtcaatcc catatccatt aaccgtcgct tcactgcgcc
     7201 ttgtccgtgg attgctacga cgatcccagt ctctcctgct tttaattgat caagagaagt
     7261 ccagctttca gaggaagcag tctttttatt gacaaagatt tgacctagta tttcccgact
     7321 tactgccact cgatgacttt ttaccagtaa aatggcattg ttgcctgata aattgacgac
     7381 gacgatttta cttccggcaa cgatcccgat attttgcaaa tggcgttttg ttttttcttc
     7441 tgtttcgatt cgttcgactt gataaacgaa attctgcttt gcctgcgcta aggtctgcat
     7501 attgattcct ctcttgtgaa ataaatcatg tattaaaatc attcattata tgttctaatt
     7561 ttcttacgtg ttacgcaaat aaatgagaat cattctcaca tagaatactc tcggtaattg
     7621 agaatgtcaa ttatacttgt gatattatga gcgaatcttt agtattgata gataaaaatg
     7681 agaaaaaaat atttcttcat agttttccac aattttttct ctattaaatt tgctattttc
     7741 ccacacgata cttgttttca gctctttttt caaaaacaca aaacttagta ttccacagtt
     7801 tccacaacac aatatgtagt gtttgttgtt tgactttttt actaagagtt ttatactatc
     7861 tattatagat aataagaatg tgttagagga ggcactcacc atgaacgtaa aaatcttttc
     7921 taaaaataat tgtatgcaat gtaagatggt caaacggttt ttaacagaaa atcaaattgc
     7981 ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta gactggttga aagcacaagg
     8041 attccaaagt gttcctgtca ttacttctga tgcagaaaca gttatcggat tccgtcctga
     8101 tcagttgcgt aaacttgcca gctaacatga ctaaaagaat caaaacgtga tacatggatt
     8161 tgaagggctt gagacagcac cagtttttgt cgcaaacttc caaatccttt tttaaagctt
     8221 aaaaacaatg caatgatttg caatcaagca tcatcaaata cgaaatgaag gttgggcgta
     8281 cgtatgagtc ttaaaaattt acaagatgtc agttatttca agctgaataa tgaaatcaat
     8341 cggccagtca atggtcaaat ccctttgaat aaggataaag aagctttagt tgcctttttt
     8401 gaggaaaatg tcaaaccaaa tacgatgact tttccatcta ttatggaaaa aatcaattac
     8461 ttgatcgatc aagaatatat agaagcagaa ttcattcaat tgtattcacc atcatttatt
     8521 gaaaagctat atcaattttt ggacgagcaa catttttctt tcaaatcttt tatggctgct
     8581 tacaaatttt attctcaata tgcgttgaaa accaacgacg gttccgctta tctggaaact
     8641 tatgaagatc gagtagcctt caatgcgctt tactttgcga acggggatga agaattagca
     8701 cttgctcttg cagacgaaat gatccatcaa cgatatcagc cagctacacc ttctttcttg
     8761 aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca agtcactgac
     8821 gatatgaact ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg tattggtggc
     8881 ggcgtaggga ttactttatc aaatcttcgt gaagccggcg ctcctatcaa aggatatgag
     8941 ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg aagatagttt cagctattct
     9001 aatcaattag gtcagcgtca aggagctggc gtggtttatt tgaacgtttt ccatccagac
     9061 attattgctt tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg cgtaaaaact
     9121 ctttctcttg gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa aaacgaagat
     9181 atgtatttat ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt ctcttatgta
     9241 aatattacag ctgagtatga tcatctagtt gcaaatccaa acattcgcaa acaaaaaatc
     9301 aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac aagaatctgg ctatccttac
     9361 gtaatcaata ttgatacagc gaaccgcgag aatccaatcg atggcaagat catcatgagt
     9421 aatttatgtt cagagatttt acaagttcaa acgccttctg tgattaacgg caaacaggaa
     9481 tatgaagtat taggaacaga tatctcatgt aacttaggct cgacaaatat cgtgaatttg
     9541 atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt gacatttatt
     9601 acagatgcat ctgatatcga tgtcgtacca ccgatccaaa acggaaataa gctgaatcat
     9661 acgattggat taggtgccat gggcttacac acttatcttg caaaagaaca aatggaatat
     9721 ggctcagagg attcattaga ctttacaaat atttacttca tgctgttgaa ttattggaca
     9781 cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag ttttccataa ttttgaaaaa
     9841 tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt ccagccaaaa
     9901 tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc caactgctga agattggaat
     9961 gctttgaaac aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt ggcagtagca
    10021 ccaaatggct caatttcata tatcaacgat acgagtgcaa gtattcatcc tatcactcgc
    10081 atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt attacccagc gccttatcta
    10141 tccaatgaga cgattccgta ttacacttct gcttatgata tggacatgcg taaagtaatc
    10201 gatgtttatg caacggcaca acaacacgtc gatcaaggca tgagtttgac tttattcatg
    10261 cgttctgaaa tcccagaagg catttatgaa tggaagacaa caacgaagca aacgacacgt
    10321 gatttgaata ttttaagaca ttacgctttc cacaaaggaa tcaaatccat ttattacgta
    10381 cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag ctgcgtgatc
    10441 taatatcata gatagattcg cttattcata ttttaattaa aggagcattc tcatgtcaga
    10501 aacatattat gaagcaataa actggaatga aattgaagat atcattgata aatccacttg
    10561 ggagaagctg acagagcaat tttggctaga tacacgtatc ccactttcaa atgacctaga
    10621 tgattggcgt acattgtctc aattggaaaa agatacagta ggacatgtat ttggcggttt
    10681 gacactgcta gatactgtcc aatctgaaag tggcatggat cagttgcgta aagatgtccg
    10741 cactccccat gaagaagccg ttttgaacaa tattcaattc atggaatccg ttcatgccaa
    10801 aagttattct tctattttta gtacactgaa tacgaaaaaa gaaatcgaag aaatttttga
    10861 ctggacaaat acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg aaatctacaa
    10921 acacggtaca ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat tcttgttcta
    10981 ttcaggtttt tatactcctc tttattattt aggaaacaac aaattggcta acgtagcaga
    11041 gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga acctacatcg gttacaaatt
    11101 ccaattagga ttcaacgagt tacctgaagc agaacaagaa acgttaaaag actggatgta
    11161 caatcttctt tatgagcttt acgaaaacga agaacgttat acagaagaat tatacgatcc
    11221 aatcggctgg acagaagaag tcaagacatt cctacgctat aatgccaaca aagcattgat
    11281 gaacctaggg atggatccac tctttccaga tactgccaat gatgtcaacc caatcgtaat
    11341 gaacgggatt tcaacaggca caagcaacca tgacttcttc tcacaagtcg gaaacggcta
    11401 tttgttaggt catgtagaag cgatgaaaga cgacgattat ttgattggat tggaataagg
    11461 atttatttta attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac agatatttta
    11521 aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac ttttggactg
    11581 tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg gatatttaat
    11641 attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa tattcctgcg
    11701 acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt cattgatttc
    11761 ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa aatcgacaac
    11821 gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc tggtaccgct
    11881 ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac cgcaagcaac
    11941 aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc aataatcaaa
    12001 gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc ttttaccgtt
    12061 tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc aataaatttg
    12121 tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa aataaaaaat
    12181 tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa tatgatattg
    12241 aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt gaaattttta
    12301 aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc atacattggc
    12361 aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt catgacagtt
    12421 aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac tctcgggatc
    12481 cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa accagcaaca
    12541 agcaatggta aaaaaattgc cgaaagcatt ttcaatattg gactaaaaat attcggcatt
    12601 tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca gaaaaataat
    12661 tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat acttctattc
    12721 tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa acaaaaaaaa
    12781 taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg agtagtgaca
    12841 aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg catcttcgta
    12901 ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg cttattactt
    12961 gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg actaaatgca
    13021 actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa gtgagccctt
    13081 ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg aattttacgt
    13141 caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta agaaaatctt
    13201 tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt acatggatca
    13261 cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc gcaatggaat
    13321 agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa gcggcagtcg
    13381 taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag aaaccgacac
    13441 cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca gcaagctgtg
    13501 gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc aatgcaatga
    13561 tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga tttttatatt
    13621 gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt aaaataatct
    13681 ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata tatcatgtct
    13741 gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga ctgtgacaaa
    13801 atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc ctcggtatta
    13861 ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa tactcaggtc
    13921 aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt acttgctgct
    13981 tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc tggcaaatga
    14041 ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc gccaaatgcc
    14101 atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac agcttttgtt
    14161 ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga ttctgttaag
    14221 tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc gatatgtcca
    14281 ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat ttgatcatac
    14341 tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt ttctttgaat
    14401 ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc atctgaaccg
    14461 cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc caaatcgctt
    14521 gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc taatactttt
    14581 gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc ttcttcagcg
    14641 ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat agcccaatta
    14701 tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt tttttcattt
    14761 tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc aaacaatcca
    14821 tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc agttttttta
    14881 tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc aatttggcga
    14941 caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc ttgggctgat
    15001 tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg agatcttacc
    15061 agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg atcaatatgt
    15121 ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg caaaagaata
    15181 cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct tcgatgattc
    15241 cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt tttatcaaat
    15301 cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag gtttagattc
    15361 actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa aactactttt
    15421 agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg atcagcatat
    15481 ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga tttcccatga
    15541 ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg gaaagattac
    15601 taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc gcgaaagcta
    15661 tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt atattcgaaa
    15721 atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat tagataaaat
    15781 cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc cattctcgcc
    15841 tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg atcgaccgct
    15901 acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc gaggcttcaa
    15961 cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa aaataagtgg
    16021 cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc tgtactggga
    16081 aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag cgactatcaa
    16141 agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa gtgaacctct
    16201 cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga caatgaacca
    16261 aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa cgaaagaaag
    16321 cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc atgaacaaga
    16381 attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa cgtttgattg
    16441 aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct tctgaataaa
    16501 tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta
    16561 tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc tcttttcgtc
    16621 tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa gaagaacgca
    16681 catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg ctgccacatt
    16741 gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat aaataca
//
LOCUS       DAZAJZ010000062        16714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000062 DAZAJZ010000000
VERSION     DAZAJZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.062"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACG86D_001944"
     CDS             complement(251..1462)
                     /locus_tag="ACG86D_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141080.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACG86D_001945"
     CDS             complement(1581..2216)
                     /locus_tag="ACG86D_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN3141081.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACG86D_001946"
     CDS             complement(2352..3005)
                     /locus_tag="ACG86D_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141082.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACG86D_001947"
     CDS             complement(3108..3314)
                     /locus_tag="ACG86D_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFN3141083.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACG86D_001948"
     CDS             complement(3390..4136)
                     /locus_tag="ACG86D_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFN3141084.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACG86D_001949"
     CDS             4296..4520
                     /locus_tag="ACG86D_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFN3141085.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACG86D_001950"
     CDS             4544..5305
                     /locus_tag="ACG86D_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN3141086.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACG86D_001951"
     CDS             5317..5574
                     /locus_tag="ACG86D_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141087.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACG86D_001952"
     CDS             5666..5917
                     /locus_tag="ACG86D_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFN3141088.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5917..6066
                     /locus_tag="ACG86D_001953"
     CDS             5917..6066
                     /locus_tag="ACG86D_001953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141089.1"
                     /translation="MFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDEAEYRAKKP
                     TEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACG86D_001954"
     CDS             complement(6180..6587)
                     /locus_tag="ACG86D_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141090.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACG86D_001955"
     CDS             6748..6972
                     /locus_tag="ACG86D_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141091.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACG86D_001956"
     CDS             6982..7176
                     /locus_tag="ACG86D_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141092.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACG86D_001957"
     CDS             7179..8201
                     /locus_tag="ACG86D_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN3141093.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACG86D_001958"
     CDS             8164..8979
                     /locus_tag="ACG86D_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFN3141094.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACG86D_001959"
     CDS             8997..9779
                     /locus_tag="ACG86D_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141095.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACG86D_001960"
     CDS             9779..10627
                     /locus_tag="ACG86D_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3141096.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACG86D_001961"
     CDS             10624..10866
                     /locus_tag="ACG86D_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141097.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACG86D_001962"
     CDS             11009..11377
                     /locus_tag="ACG86D_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN3141098.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACG86D_001963"
     CDS             11392..11553
                     /locus_tag="ACG86D_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3141099.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACG86D_001964"
     CDS             11550..11876
                     /locus_tag="ACG86D_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141100.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACG86D_001965"
     CDS             11873..12688
                     /locus_tag="ACG86D_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFN3141101.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACG86D_001966"
     CDS             12690..12815
                     /locus_tag="ACG86D_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141102.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACG86D_001967"
     CDS             12827..13138
                     /locus_tag="ACG86D_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN3141103.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACG86D_001968"
     CDS             13135..13380
                     /locus_tag="ACG86D_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141104.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACG86D_001969"
     CDS             13377..13793
                     /locus_tag="ACG86D_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN3141105.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACG86D_001970"
     CDS             13790..14128
                     /locus_tag="ACG86D_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141106.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACG86D_001971"
     CDS             14125..14355
                     /locus_tag="ACG86D_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141107.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACG86D_001972"
     CDS             14362..14565
                     /locus_tag="ACG86D_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN3141108.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACG86D_001973"
     CDS             14562..14858
                     /locus_tag="ACG86D_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141109.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACG86D_001974"
     CDS             14935..15348
                     /locus_tag="ACG86D_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN3141110.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACG86D_001975"
     CDS             15361..15747
                     /locus_tag="ACG86D_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141111.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACG86D_001976"
     CDS             16298..16525
                     /locus_tag="ACG86D_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141112.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACG86D_001977"
     CDS             16522..>16714
                     /locus_tag="ACG86D_001977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141113.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAZAJZ010000063        16547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000063 DAZAJZ010000000
VERSION     DAZAJZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.063"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1197)
                     /locus_tag="ACG86D_001978"
     CDS             complement(<1..1197)
                     /locus_tag="ACG86D_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN3141114.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQP"
     gene            complement(1184..2872)
                     /locus_tag="ACG86D_001979"
     CDS             complement(1184..2872)
                     /locus_tag="ACG86D_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141115.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3018..4655
                     /locus_tag="ACG86D_001980"
     CDS             3018..4655
                     /locus_tag="ACG86D_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141116.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4800..5540)
                     /gene="truA"
                     /locus_tag="ACG86D_001981"
     CDS             complement(4800..5540)
                     /gene="truA"
                     /locus_tag="ACG86D_001981"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN3141117.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5731..6366)
                     /locus_tag="ACG86D_001982"
     CDS             complement(5731..6366)
                     /locus_tag="ACG86D_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN3141118.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6513..7733)
                     /gene="sstT"
                     /locus_tag="ACG86D_001983"
     CDS             complement(6513..7733)
                     /gene="sstT"
                     /locus_tag="ACG86D_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN3141119.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            7952..8950
                     /locus_tag="ACG86D_001984"
     CDS             7952..8950
                     /locus_tag="ACG86D_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141120.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9146..10981
                     /locus_tag="ACG86D_001985"
     CDS             9146..10981
                     /locus_tag="ACG86D_001985"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3141121.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(11041..11757)
                     /locus_tag="ACG86D_001986"
     CDS             complement(11041..11757)
                     /locus_tag="ACG86D_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN3141122.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(11984..12526)
                     /locus_tag="ACG86D_001987"
     CDS             complement(11984..12526)
                     /locus_tag="ACG86D_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFN3141123.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12531..12758)
                     /locus_tag="ACG86D_001988"
     CDS             complement(12531..12758)
                     /locus_tag="ACG86D_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFN3141124.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(12837..14717)
                     /locus_tag="ACG86D_001989"
     CDS             complement(12837..14717)
                     /locus_tag="ACG86D_001989"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3141125.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(14906..15520)
                     /locus_tag="ACG86D_001990"
     CDS             complement(14906..15520)
                     /locus_tag="ACG86D_001990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3141126.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            15662..16222
                     /locus_tag="ACG86D_001991"
     CDS             15662..16222
                     /locus_tag="ACG86D_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3141127.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         5301 a         3115 c         2574 g         5557 t
ORIGIN      
        1 cggctgagaa attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa
       61 taacaaaaca gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt
      121 gaaaaatatc atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca
      181 aggaaattgt aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt
      241 tgtagctagc caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt
      301 tttaaataaa atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt
      361 agttaagtcg ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata
      421 taaatttgtc acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata
      481 ccaaaaagca ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat
      541 agcaataaaa tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat
      601 aattaatgct gtactcatta atgttgatag tacatggctg tacactagca aactcatact
      661 caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata
      721 aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta
      781 gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa atgccgcttt
      841 tttttgtttt aaaaaccgct cgccataatg ataagctacg agtgttgtac acaagttcaa
      901 taaccaaacg taaataatat agcttacaat caaatttttt gtcatccaat ataacattac
      961 agctggaaaa aaagtcagaa agggataaaa ataatttaca ccatatccag tataattgaa
     1021 tgtctcataa ttaatcggac tggtaaaaat agtcgataac cccttgattc gagataagtg
     1081 aaaagaaaga tctccagtgg tataaatact ctgtgtattt ttgaaaaaga tgaaagaata
     1141 tatactacta actataatta atccactcaa tattactact tttttattta ttttcattct
     1201 tcttgtttat ctccttttta gtcacatatc gagaatcttt caacttatta ggtgcaaatc
     1261 gtttaaaact tgaatacacc actaaacaat aagtcagaat actgatccat aaagccatga
     1321 gagcagttgg agtatatttt gttgtaattt ctatcttatt ttctccagca tttacatcca
     1381 cattcactaa gccatgtttt ttataagagt catttacgac tttaccgttt acttttacct
     1441 gtgtacctaa ataagcatat actggtactg tcactttttg ctttttgttg cttgccactg
     1501 tgtaagtaat gttattatct gcagctttat acttcccttt ttcccacgat tctcctactt
     1561 ttacttcatg tttttctaag tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt
     1621 ataatcctcc catatctgat gttgtataac gtgggagtgt ttcttcattg ataacaatat
     1681 gaccgtgttt tgattggata acttttgcgt aattttcttt tgctgctata gacgtgacga
     1741 gtagaaacac cacagtagcg ccaatgatta accatgaata ttttttattc atagtctttt
     1801 tcataaaaac aagcgttatg atcattgaag aaaaaaagat ttggatacct aagatacgcc
     1861 aaggaaactg gagtaagctt atcggtgttt tttgaaacaa aaaccatgga aataaattag
     1921 ttacgcatac ccatgtagaa actacgccaa catagatgat tttttctttt ttctccattt
     1981 ctttcagtat gaataaagga aaaatcaacg caaccaatcc taataacccc atagtgtatg
     2041 ttgtaatatc gttatttaac gcttgaatga tactatctga aatagaaagt gcttctagct
     2101 gcaaatctgt tttgaaggga ctgtgtattt cttgataatt catctgctgc aacataggat
     2161 accaaaaata gctagttaat gcacaagtca aaaaaacagc ctttactaaa ttcacgacca
     2221 caaattttat atcgtttttt atttgcttgc ttaaaaacaa acgaactata atcatacaag
     2281 caatcaatgc actacacaat agtgtagtta agacatgagt atacacaagt agacttatac
     2341 tgacagataa agcgatgact tttttttctc tattgaatat caatccataa gcataatata
     2401 aaacagctgg gataatcgta agagcgattg cttctgcaat tgcggatcga taatagatat
     2461 caactgttcg ataagttgca aaagtatata aacaactaaa taaaaaagca gcatctatct
     2521 ttttaaaaaa cttttctcca taataaaaac taatcagaat cgtacaaata tttaataacc
     2581 aaatatatag aacataactt aatattaagt tatttgaaat tccataaaaa acgactgcag
     2641 gaattattgt aagaaaagga taaaaatagt tcagtccatc tccataattg ttaaatgttg
     2701 tatagttgat tggcccttca aaaatagaag ataacccttt gattcgtgaa agatgaaaag
     2761 ataaatcacc tgttgtataa atttcttgag catttctcat aaaatttact gaatataaag
     2821 tactaagtaa gataagaact actaaaagaa atgctttttt acttattttc atagatttgt
     2881 tgttctcctt ttttaattct attttcagta tactagaatt gtcttttttt aataagtttt
     2941 gcgttaaatt tttcatagca ctagtatgat atactagaaa agaatcaatt tttattactt
     3001 ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt
     3061 tatttctaag ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg
     3121 tcaatcagga tacttacttt catttatctc gaatcatagg actagataat gtatggagtt
     3181 ctccagtaaa cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat
     3241 ggcttactct ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt
     3301 ataatatcta ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga
     3361 aacaaataaa aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg
     3421 cctatcgtgc cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat
     3481 ttttgccttt gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt
     3541 actggttgtc tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg
     3601 tttctgtatt tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg
     3661 ctagactctt aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct
     3721 taattccttt tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat
     3781 taagtggtat ggctcctagt gatatgctga cacatatttt aaataataac tataacaact
     3841 atactattgg attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat
     3901 taactgctga tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca
     3961 atttatttcc ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc
     4021 ggttaaatgg tttttcttct ttatttatag cctatacgat gagtatagtg atctattata
     4081 ttttttctac taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat
     4141 tgcatctctc aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag
     4201 cacctgaaga cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag
     4261 agtcaatcta tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa
     4321 taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata
     4381 ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta
     4441 atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc
     4501 caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat
     4561 atttatctat cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa
     4621 aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa agacttttta
     4681 gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca
     4741 ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt
     4801 tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt
     4861 tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg
     4921 atttctagta atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca
     4981 aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact
     5041 gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat
     5101 ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg
     5161 tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc
     5221 accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata
     5281 tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt
     5341 gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca
     5401 ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg
     5461 gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt
     5521 gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct
     5581 acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac
     5641 aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc
     5701 cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacca
     5761 ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag
     5821 taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa
     5881 actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata
     5941 gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat
     6001 attatggacc catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc
     6061 tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa
     6121 cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat
     6181 ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa
     6241 tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg
     6301 agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa
     6361 atccatgata cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt
     6421 tatcttgatt taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc
     6481 gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa
     6541 tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta
     6601 aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata
     6661 ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat
     6721 gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa
     6781 gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt
     6841 acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca
     6901 cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc
     6961 atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct
     7021 ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat
     7081 gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga
     7141 tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc
     7201 cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga
     7261 tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc
     7321 cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca
     7381 ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt
     7441 tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa
     7501 agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt
     7561 ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc
     7621 aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata
     7681 ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa
     7741 aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc
     7801 aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct
     7861 aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat
     7921 taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg
     7981 tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg
     8041 tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac
     8101 aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa
     8161 tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca
     8221 ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt
     8281 ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag
     8341 tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac
     8401 agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac
     8461 aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg
     8521 acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa
     8581 ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt
     8641 cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc
     8701 gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt
     8761 atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt
     8821 atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac
     8881 atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt
     8941 ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa
     9001 aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac
     9061 tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc
     9121 agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg
     9181 gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct
     9241 gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg
     9301 gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa
     9361 aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc
     9421 agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct
     9481 attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat
     9541 tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt
     9601 tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa
     9661 gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa
     9721 caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg
     9781 aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt
     9841 aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc
     9901 taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac
     9961 aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg
    10021 actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct
    10081 agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat
    10141 ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga
    10201 caggttctat tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag
    10261 cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac
    10321 ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta
    10381 ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga
    10441 attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca
    10501 agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc
    10561 aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt
    10621 catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc
    10681 tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta
    10741 tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag
    10801 cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca
    10861 agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata
    10921 tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta
    10981 acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca
    11041 ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
    11101 ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
    11161 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
    11221 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
    11281 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg
    11341 agggacttta agaccatata agtccacact catttgattc gtataacttt tgtattgttg
    11401 tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt
    11461 ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga
    11521 agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga
    11581 ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt
    11641 agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt
    11701 ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta
    11761 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
    11821 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
    11881 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
    11941 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
    12001 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
    12061 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
    12121 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
    12181 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
    12241 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
    12301 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
    12361 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
    12421 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
    12481 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
    12541 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
    12601 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
    12661 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
    12721 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
    12781 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
    12841 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
    12901 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
    12961 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
    13021 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
    13081 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
    13141 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
    13201 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
    13261 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
    13321 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
    13381 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
    13441 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
    13501 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
    13561 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
    13621 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
    13681 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
    13741 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
    13801 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
    13861 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
    13921 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
    13981 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
    14041 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
    14101 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
    14161 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
    14221 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
    14281 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
    14341 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
    14401 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
    14461 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
    14521 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
    14581 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
    14641 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
    14701 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
    14761 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
    14821 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
    14881 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
    14941 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
    15001 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
    15061 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
    15121 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
    15181 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
    15241 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
    15301 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
    15361 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
    15421 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
    15481 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
    15541 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
    15601 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
    15661 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
    15721 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
    15781 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca
    15841 attagaaagc aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag
    15901 ctatgaggaa ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg
    15961 cttatttatc ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga
    16021 agaaaactat ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat
    16081 gagattcttt actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga
    16141 aagcaattac aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca
    16201 catagagaaa atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac
    16261 cttctcgtat aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta
    16321 aaatttctat aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa
    16381 gtgatatatc ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct
    16441 aatttattac aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat
    16501 catccagttt attgaattct tctcgtttac ctcaattcat tttcaag
//
LOCUS       DAZAJZ010000064        16316 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000064 DAZAJZ010000000
VERSION     DAZAJZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.064"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..1659
                     /locus_tag="ACG86D_001992"
     CDS             232..1659
                     /locus_tag="ACG86D_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141128.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1751..2116)
                     /locus_tag="ACG86D_001993"
     CDS             complement(1751..2116)
                     /locus_tag="ACG86D_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN3141129.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2113..2478)
                     /locus_tag="ACG86D_001994"
     CDS             complement(2113..2478)
                     /locus_tag="ACG86D_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN3141130.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2670..2918)
                     /locus_tag="ACG86D_001995"
     CDS             complement(2670..2918)
                     /locus_tag="ACG86D_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141131.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2917..3045
                     /locus_tag="ACG86D_001996"
     CDS             2917..3045
                     /locus_tag="ACG86D_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN3141132.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3102..4796
                     /locus_tag="ACG86D_001997"
     CDS             3102..4796
                     /locus_tag="ACG86D_001997"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN3141133.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4896..5069
                     /locus_tag="ACG86D_001998"
     CDS             4896..5069
                     /locus_tag="ACG86D_001998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141134.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5447..6580
                     /locus_tag="ACG86D_001999"
     CDS             5447..6580
                     /locus_tag="ACG86D_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN3141135.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6611..6931
                     /locus_tag="ACG86D_002000"
     CDS             6611..6931
                     /locus_tag="ACG86D_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN3141136.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6960..7424)
                     /locus_tag="ACG86D_002001"
     CDS             complement(6960..7424)
                     /locus_tag="ACG86D_002001"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3141137.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7556..8809)
                     /locus_tag="ACG86D_002002"
     CDS             complement(7556..8809)
                     /locus_tag="ACG86D_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3141138.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8838..9155)
                     /locus_tag="ACG86D_002003"
     CDS             complement(8838..9155)
                     /locus_tag="ACG86D_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3141139.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9181..9501)
                     /locus_tag="ACG86D_002004"
     CDS             complement(9181..9501)
                     /locus_tag="ACG86D_002004"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3141140.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9965..11380)
                     /locus_tag="ACG86D_002005"
     CDS             complement(9965..11380)
                     /locus_tag="ACG86D_002005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3141141.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11397..12713)
                     /locus_tag="ACG86D_002006"
     CDS             complement(11397..12713)
                     /locus_tag="ACG86D_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3141142.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12863..13561)
                     /locus_tag="ACG86D_002007"
     CDS             complement(12863..13561)
                     /locus_tag="ACG86D_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN3141143.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13850..14839)
                     /locus_tag="ACG86D_002008"
     CDS             complement(13850..14839)
                     /locus_tag="ACG86D_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3141144.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14919..15752)
                     /gene="msrA"
                     /locus_tag="ACG86D_002009"
     CDS             complement(14919..15752)
                     /gene="msrA"
                     /locus_tag="ACG86D_002009"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3141145.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15927..>16316)
                     /locus_tag="ACG86D_002010"
     CDS             complement(15927..>16316)
                     /locus_tag="ACG86D_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141146.1"
                     /translation="GMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSI
                     IYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSY
                     TVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5090 a         3282 c         2758 g         5186 t
ORIGIN      
        1 aaataaaatg actgtccttc attcactaaa catattttta tttttttata ttgttaaaaa
       61 ttcgcaattt attttttaat agaattatct gttttccaaa ttttataaca taaaaaacct
      121 tttttaacgg catttatatg ctttttcaga aattaactga tgatttcaaa aagaaaacat
      181 gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa
      241 gaatatattg agaaaaatat tttaagacag ctgtttttat gtgggcaatt ctatgtaaat
      301 aaagaagtca atctagaaaa gctctccaat ctcctgcatg tttgcaaaac cactttactc
      361 aatgatatca ataacataaa aaaagaattt gaagaacaga tcgcttatac acatcgggaa
      421 aaggaccgtt acactttata tttttcagaa catatacctc gatgcaagat catgcagcaa
      481 ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt atttggaaga agatgaacct
      541 gattatcttc aattaactga atgtgaattc atctctgtca gcaaagctta ttcattaaaa
      601 aaacaagtat tagcttattt taatgactgc gggatcgaaa tcgaccgcta ctcccctcga
      661 ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt
      721 aattcttggg agctacctga atcgtttttt gaacgtgccg atcgtttcat tgaatccgta
      781 acggaaaata gtggcagatt ttatgataaa gaaaataaag aaatcttatc gattggtttt
      841 gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa
      901 gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag catgggaaaa cactgatttc
      961 caaacctatt ataaaaaaga agaattcgcc tttattttga cactattcaa tctttgtaat
     1021 tatggtttcc attcttatca gttgattgcg gaagactttc aacagctaca ccaagtgttt
     1081 attgacaata ctcccgaaat aaaagaacta gttgctacct ttgaatctca cttcaatcaa
     1141 gaattattcg gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg
     1201 gataactatc aattattcat gccagaaaaa ttttatcttt taaatgagga acagacaaat
     1261 ttatataaag aagtccagac aattttttcc agctggtcca gccagttacc ttatgatcta
     1321 cggttgaatc ctaattgcat gcgggctttt gtgatcgaat tgagcggaat tctccgtctg
     1381 acaaaagaac atctgactat ctatatcgtc acaaattcgg atgttcatta tctgatttat
     1441 cgggaagcct tggaagctgt aactactttc gattttcaag ttgcaccaac aatttattca
     1501 agtatatctg atataaagaa gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt
     1561 acattatata caccagatgc ggtccaatat gaaaatatta ttcctatttc gatcaatacg
     1621 atcgatcgag ctatcatttc agctgtgcaa aacaaataag gggctgtgac aaaaatcatg
     1681 tcacagcctc ttatttgttg aagatgccca tttctgtaac aatctcttga tacacgtcag
     1741 gctcacaacc tcacttattt tggacatcta cgggtccgat ctctttattt aaaaaataag
     1801 aaatgatggt tcggataact accaacgccg ctagttggaa aatatcctgc aaggtaggtt
     1861 tgataatcga ttcgatgatc ccagctgaaa caagaatttc caaacttaga agtatatagc
     1921 ctcccaacat ctttttaata gcattgttct cgctgactcg gtcgctttgc acttttgtat
     1981 ggacaaataa atatttcaaa aaatccttga ttgatagaca aacaccccaa accaataaac
     2041 agatagcaaa gatatcaagt cccagtacaa gtttttcgaa atatggaaga agaaactgca
     2101 tcaactctgt cgtcatccta cctctcctct tctttgtttt tctttgtctt ctatttcctg
     2161 attcaagaaa taagaaatga ctgttcgaat gataataata agtgcaagct tcaaaatatc
     2221 tgtccaagtc ggcttgataa ctgactcgat gatatcagca acgactagta cttccaagct
     2281 taacaaaata taacttgcta gcagttttct tgtatctgca ttacgagtat tacgttctgc
     2341 atagtttttc ttgacatgag accaatcaaa aagtttgaat agaacaagaa cgatcccgac
     2401 aattaagata ccgatagaac aaatatttaa aatagcaatg atccattcta gaaattcaat
     2461 taaatttgag cgaaacatat acgctaccta ctttctttta atttgttcct attcttttat
     2521 tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt tctaatgata acggttcgct
     2581 taaatttttc tttttcactc tcccattttt ctccctccat ccataaatcg tggaagtatc
     2641 caaaacaacg tagcaagtta aaataaggct tagtccgtca tcagcttttc aaagaagcac
     2701 tgtctcggtg ctttttttgt acccaaattt ctaaaaatag aaccatacac ctcctcaaat
     2761 cttttcttct tacctaccct atttttattt acaggaaaat cagacacttc ttcttttttg
     2821 tcaaaatttc ctttttcttc tcgtcctatc atttggcatt ctaataaaaa cgaactatcc
     2881 ttggatatag aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc
     2941 ggcatcggtt taggtttgct tgcccttggc gctgcatatc taggaaaaaa gacaggacta
     3001 ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa tctagcatcc tatttagttg
     3061 aatcactacg gacatacttg gaagacattg gaggaatcac aatgagaaaa agtaaagcaa
     3121 taatgattgg tgccggtatt gccaatatgg cagcggctgt ttatttgatt caagaaggca
     3181 aatggaaagg ggatcagatc accttttatt cattggatga tcacggttca aatgatggtg
     3241 caccgaccga cactgtagtg gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa
     3301 aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta gatttgatgg
     3361 atctgttaag tcgaatacct tctgttactg agccagggat gactgctgaa gaagatacga
     3421 gagattttga tgcaaaacac cgaacctttg acaaagcacg tttgatggaa ggtggcaaag
     3481 gaatcattga tggcggaaaa ctgggattca ataataaaga ccgtattctt ctgactaaac
     3541 tgattgctat gcctgattca gaagaagaaa aactagacaa tatcactatc gaagaatatt
     3601 tcaaagatga tccacacttt tttgaaacaa acttctggtt tatgtgggaa acaacatttg
     3661 ctttcagaac acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg
     3721 aattcactca aatcgaacat ctagtgggcg tcaatcgaac acgttacaat caatacgaaa
     3781 gtattatgct tcctttgatc gagtacttga aagaacaagg atgcaaaatc attttgaacc
     3841 gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca agatgaaatc actgtgactg
     3901 gtttgaaaat taccaataca ctgactcaag aagaagaata cgttgtggtc gatgaagata
     3961 cagcagttat tttcactaat gggtccatta cagattcagc tactttagga gattacgaaa
     4021 caccagctgt agaaaatatg gattatggtg cagcttctag tctctggaaa aaagccagtg
     4081 aacgtttcta caacttggga aatcctgaca aattctttgc tgatcgagat gcaagcgaat
     4141 gggtcagctt taccctgacg acgaaaaacc atttgttgtt gaatgaaatc acccgtatca
     4201 cgactcaaga accaggaaat gccttgaatt catttatttc aaccacaccg atcacgccgt
     4261 taggccaaaa agatgtcaat atgtctattg tcgtgcatca tcaaccgcat tttacgtctc
     4321 agaaaccaaa tgaaacagtt atttggggtt atttccttta tccaagacga cgtggcgaat
     4381 ttgtggataa acagtatatt aaaatgaatg gaaaagaaat gctagaagaa ctgattggac
     4441 aactttcaaa agtagatcct ggaccagtga atattcgtga aaaagaaaca gaaattttcg
     4501 acagcgtaat aaataatatc cctgtctata tgccttatgc ttccgcgttg ttcaataatc
     4561 gtgccaaaag cgatcgacca aaagtcattc ctaaacattc gaccaatctg gcctttacag
     4621 gagaatttgt cgagcagcct tatcagatga tctttactga acaaagtgct gtacgttctg
     4681 gagaaatcgc tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc
     4741 gttacgacaa agatattcct actctagctc gtgccgcaaa aaaaatgttt gaataatcaa
     4801 gaggttgtga aaaagctgcc ctgcatcaag taataagaac aaccaggcta aaacagcttc
     4861 ttatgttttt gatttggttg gacttattac cgcagatgca agcttttgaa caccgtttac
     4921 caagaggtta tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc
     4981 ttcttatgtt tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt
     5041 taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     5101 ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat
     5161 aacctctttt tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc
     5221 tcttgccaat ccaacatcat ctaacggata caactatttc aatgattacc attacactaa
     5281 aaaatcggaa acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa
     5341 gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag
     5401 tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac
     5461 ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc
     5521 aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta
     5581 ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat
     5641 cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc
     5701 ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga
     5761 tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa
     5821 tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt
     5881 tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac
     5941 agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg
     6001 ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt
     6061 aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga
     6121 aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg
     6181 tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa
     6241 agcgaaaaat ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca
     6301 agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc
     6361 ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat
     6421 tatccatcta atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca
     6481 aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa
     6541 aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt
     6601 gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga
     6661 agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat
     6721 ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt
     6781 aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac
     6841 gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat
     6901 gaagagaaca agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt
     6961 caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt
     7021 gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt
     7081 ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc
     7141 ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg
     7201 cggttatgga agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct
     7261 aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc
     7321 tcatacacaa cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact
     7381 aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac
     7441 taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat
     7501 agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag
     7561 catctaaagc ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga
     7621 tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg
     7681 ccaatacacc gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca
     7741 ctaaacctgt agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa
     7801 atggaatgaa catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa
     7861 tattgaagac acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac
     7921 gtcctactaa ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga
     7981 caaagctgtc aaagaaaggt ttgttcacga taaacggtgc gctatgtcca gctgcgattg
     8041 cattaacgtt tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat
     8101 tcgctccgtg taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag
     8161 atggataaga gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta
     8221 cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag
     8281 tgatcattgc tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag
     8341 gcattttgat gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa
     8401 tcccaacaat aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag
     8461 cgaagaaaga gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag
     8521 ctaaatgata agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc
     8581 aagcagcagc cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt
     8641 ggaacccggg aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg
     8701 gcatactcac cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg
     8761 atgctacagg tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct
     8821 cttttcttta ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct
     8881 aaatctgtaa aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg
     8941 acttcaacgt gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct
     9001 tggactaaag attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt
     9061 ttcgcagcag caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga
     9121 cccatgatgg cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta
     9181 ttatttgttt attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata
     9241 gtcagccata ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg
     9301 attttgcata aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag
     9361 ttgattatcc gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc
     9421 tgctgccttt tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag
     9481 catgattgtt ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa
     9541 gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc
     9601 tataccaagt tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt
     9661 tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact
     9721 tttttcaaat aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa
     9781 aaacataaga tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc
     9841 acaacctctc aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg
     9901 attcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt
     9961 ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat
    10021 accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta
    10081 atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca
    10141 tatagccttc gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc
    10201 cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt
    10261 catattctct tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt
    10321 ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat
    10381 tcattttctt cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct
    10441 cttcattcca ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa
    10501 ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa
    10561 tcgattgttt ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat
    10621 caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag
    10681 aattggatcg agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat
    10741 ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca
    10801 tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg
    10861 tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat
    10921 caaaggcaat ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat
    10981 catatttttc caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta
    11041 cgtctgcttc gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta
    11101 ctatacagtt ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg
    11161 tcagatggat ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg
    11221 ctacggctgg gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta
    11281 taaacgaatc ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg
    11341 cagcaccaag aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag
    11401 atcccatcat tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa
    11461 acaataatac aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa
    11521 gcattgacga tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct
    11581 aaagcggttg caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata
    11641 gctaaaattg gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag
    11701 atcccaggag caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac
    11761 aatactgcaa tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc
    11821 caaaatggca tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt
    11881 acagcaattg ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg
    11941 ataccaatca cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct
    12001 tgtacgacac cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt
    12061 cctgtaagtt ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc
    12121 gggaataaag cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc
    12181 tgtagtttct tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac
    12241 attcccaaaa ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata
    12301 acagtctgat tttcattcac taattcataa acagttggat taacagataa ataagacatc
    12361 actgcgatga taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct
    12421 atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg
    12481 ataggcttta attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag
    12541 tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat
    12601 gcagcaaatg cgttttttat caccatgata taccttaaat tattgattct cgttgccacg
    12661 ttccctaaaa cattggctaa tttatccata aaactcattc cagatcccct cattcctgct
    12721 tcttctcctt gacgtcatct ctttttgtct attagtcagc gcttacattt cgatgaacag
    12781 aaactattta ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc
    12841 ataaaataca tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt
    12901 ctctccgatt tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc
    12961 tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga
    13021 tatcataatt tttattaaac aaagcaattg attcattaat gattgggata atatgcagca
    13081 aggctacgtt tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat
    13141 gttcttgtgt gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga
    13201 cgatttgatt cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat
    13261 atctcagttc aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga
    13321 gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt
    13381 caaggatatt cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct
    13441 ctaaataatc ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt
    13501 cctttaaata tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca
    13561 aaccacatac ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat
    13621 catctgatga ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga
    13681 agatatttac cataaaaaag actaggataa aattcactcc tagccttcga tcatttttat
    13741 tttgaacgac aaaaaatatg atgttcaata acagacgatc ttcattcgat tcttgatgtc
    13801 atcttttgtt ttttttaata tatggataaa aagtttgatt acatcgtttc tattctagaa
    13861 tttggtcgat tctcaattct tcttctggtg agaaatctaa attatccatc attcgaacac
    13921 tttcttgtaa ctgacttaac cgacttgctc cgaccagtac acttgtaaca gatttttgtc
    13981 gcaaattcca tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg gcgattgttt
    14041 gcagtgcttt gattttttcc agagttgatc caacttgttc ttctgaaagg aatggtattt
    14101 ccttacgatg tgctctagaa tcttcaggaa taccatgtag ataacgattt gtcagcaacc
    14161 cttgagcaag cggactgaac gtgattgccc ctagcccttc ttcttctaat actggagata
    14221 gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg catctgatga ataatgaacg
    14281 gtgcttcctt tctttttaaa atttcagtca ttttttttgt atcttctcca ttatagttgg
    14341 atataccaat atataatgct tttccttgtc ttacaagttg catcaaagct tctgctgttt
    14401 cttccaaagg tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc
    14461 ccattctttg gagactttgg tcacaacttg aaatgatgga tttacgagag ccccattccc
    14521 catatggccc ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt
    14581 aggcttgaaa atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc
    14641 ctccataatt attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa
    14701 tctctctttg attatggata ggatcataat caccaaaatt ttgccataat ccgagagata
    14761 aaatcggtaa tttcaaacct gaatgaccgg atttacgata ttccattttt tcataacgtg
    14821 cactatctgc aatatacaat ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt
    14881 tttctatttt tagtttacta cattttaaag aaaaggagtt aatcaaaatg attttttcgt
    14941 atattttttt gccatttgtt ttttactcgt tgatagatca gaaattgatt acgtgcttgc
    15001 tggatttttt tataacgttt cggttgtttt ttgtaaaatt gctgatgata gttttctgct
    15061 ggccaaaacg taccggctgg ttcgattttt gtgacgattg gttggtggaa agttcctgaa
    15121 ctgtctagtt tctgttttga ttgttccgct aattcttttt gtcgttcgac ttgataaaaa
    15181 atcactggac gatattgctt ccctctatct tgaaattgtc caaatgtatc tgtgggatca
    15241 gtaatttgcc aatagattgt cagcaactcg ctataactta tttctcttgt gtcataaatg
    15301 atctccacag cttccacatg acctgtgtaa ccaccactta cttgatcata atttggtttt
    15361 tcaacatgtc cgcccgtgta tcctgacaaa acagatagga tccctttttt cgattcaaac
    15421 ggctcgacca tacaccagaa acatccccct gcaaatactg cacgttcttg atagtcagca
    15481 tcttgcatgg catgcttgga aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc
    15541 aataaataaa agtccattac atcaggcgtc agattcattc ggatagctaa tggacgtagt
    15601 gttgcttcta gttttgataa ctgttcttta acggatatat tttctcttat gtcattcttt
    15661 gtctgaatca atacttttcg ttcccaatct cgtgtattcg gattcaaaat cagattataa
    15721 agtgtttgaa tgaatatttg ttcctcatcc atagattctc ctcctttccg aagaagacaa
    15781 gctagtgact ttagctgtaa tttatcataa agtgaggaaa taagcgatta cttcatcccc
    15841 ctacagattt ttcgtataaa aaagagagaa tccttatcga cagattctcc tttttaatca
    15901 atttatttta taaatgaaaa gttaatttac cttttatgga aaactatttt aaaaaataaa
    15961 cataaatatg cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg
    16021 cttactttaa tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc
    16081 acctgctcct ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca
    16141 ggaagtgtga ttacttggct cttctcatta gttactctat atatgataga actaccagtt
    16201 gttttataag gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc
    16261 tccttattct ccaatgaagc gataggagaa taatcatata aaaaataatc cattcc
//
LOCUS       DAZAJZ010000065        15995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000065 DAZAJZ010000000
VERSION     DAZAJZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.065"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG86D_002011"
     CDS             226..894
                     /locus_tag="ACG86D_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN3141147.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG86D_002012"
     CDS             complement(1094..1414)
                     /locus_tag="ACG86D_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141148.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG86D_002013"
     CDS             complement(1451..2833)
                     /locus_tag="ACG86D_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN3141149.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADQANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG86D_002014"
     CDS             complement(2989..4176)
                     /locus_tag="ACG86D_002014"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN3141150.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG86D_002015"
     CDS             complement(4183..5214)
                     /locus_tag="ACG86D_002015"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3141151.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACG86D_002016"
     CDS             complement(5260..6684)
                     /locus_tag="ACG86D_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN3141152.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACG86D_002017"
     CDS             6859..7764
                     /locus_tag="ACG86D_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN3141153.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG86D_002018"
     CDS             7855..8532
                     /locus_tag="ACG86D_002018"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN3141154.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG86D_002019"
     CDS             8529..8699
                     /locus_tag="ACG86D_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141155.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG86D_002020"
     CDS             8813..9709
                     /locus_tag="ACG86D_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3141156.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG86D_002021"
     CDS             complement(9764..11134)
                     /locus_tag="ACG86D_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3141157.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG86D_002022"
     CDS             11372..12118
                     /locus_tag="ACG86D_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3141158.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG86D_002023"
     CDS             12115..12954
                     /locus_tag="ACG86D_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3141159.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="ACG86D_002024"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="ACG86D_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="ACG86D_002025"
     CDS             14045..14797
                     /locus_tag="ACG86D_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141160.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="ACG86D_002026"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="ACG86D_002026"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN3141161.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5192 a         2995 c         3223 g         4585 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttggtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gtttt
//
LOCUS       DAZAJZ010000066        15900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000066 DAZAJZ010000000
VERSION     DAZAJZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.066"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG86D_002027"
     CDS             complement(52..522)
                     /locus_tag="ACG86D_002027"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN3141162.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG86D_002028"
     CDS             complement(681..1139)
                     /locus_tag="ACG86D_002028"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN3141163.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG86D_002029"
     CDS             complement(1310..2455)
                     /locus_tag="ACG86D_002029"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN3141164.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG86D_002030"
     CDS             complement(2468..3385)
                     /locus_tag="ACG86D_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN3141165.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG86D_002031"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG86D_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN3141166.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG86D_002032"
     CDS             4477..4647
                     /locus_tag="ACG86D_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141167.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACG86D_002033"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACG86D_002033"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN3141168.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACG86D_002034"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACG86D_002034"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN3141169.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACG86D_002035"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACG86D_002035"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN3141170.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACG86D_002036"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACG86D_002036"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN3141171.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACG86D_002037"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACG86D_002037"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN3141172.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACG86D_002038"
     CDS             9222..10223
                     /locus_tag="ACG86D_002038"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN3141173.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACG86D_002039"
     CDS             10220..11581
                     /locus_tag="ACG86D_002039"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN3141174.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACG86D_002040"
     CDS             11541..12692
                     /locus_tag="ACG86D_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3141175.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACG86D_002041"
     CDS             12667..13836
                     /locus_tag="ACG86D_002041"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3141176.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACG86D_002042"
     CDS             14011..15390
                     /locus_tag="ACG86D_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3141177.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="ACG86D_002043"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="ACG86D_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2803 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAZAJZ010000067        15706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000067 DAZAJZ010000000
VERSION     DAZAJZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.067"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..3208
                     /locus_tag="ACG86D_002044"
     CDS             41..3208
                     /locus_tag="ACG86D_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN3141178.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            3276..3773
                     /locus_tag="ACG86D_002045"
     CDS             3276..3773
                     /locus_tag="ACG86D_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN3141179.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            3792..4256
                     /locus_tag="ACG86D_002046"
                     /pseudo
     CDS             3792..4256
                     /locus_tag="ACG86D_002046"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            4253..5011
                     /locus_tag="ACG86D_002047"
     CDS             4253..5011
                     /locus_tag="ACG86D_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3141180.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(5097..5837)
                     /locus_tag="ACG86D_002048"
     CDS             complement(5097..5837)
                     /locus_tag="ACG86D_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3141181.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5898..7151
                     /locus_tag="ACG86D_002049"
     CDS             5898..7151
                     /locus_tag="ACG86D_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN3141182.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            7473..7700
                     /locus_tag="ACG86D_002050"
     CDS             7473..7700
                     /locus_tag="ACG86D_002050"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN3141183.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            7823..8149
                     /locus_tag="ACG86D_002051"
     CDS             7823..8149
                     /locus_tag="ACG86D_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN3141184.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            8217..8930
                     /locus_tag="ACG86D_002052"
     CDS             8217..8930
                     /locus_tag="ACG86D_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN3141185.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            9240..10090
                     /gene="licT"
                     /locus_tag="ACG86D_002053"
                     /pseudo
     CDS             9240..10090
                     /gene="licT"
                     /locus_tag="ACG86D_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            10211..12076
                     /locus_tag="ACG86D_002054"
     CDS             10211..12076
                     /locus_tag="ACG86D_002054"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3141186.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            12090..13533
                     /locus_tag="ACG86D_002055"
                     /pseudo
     CDS             12090..13533
                     /locus_tag="ACG86D_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            13641..14342
                     /locus_tag="ACG86D_002056"
     CDS             13641..14342
                     /locus_tag="ACG86D_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3141187.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            14468..14980
                     /locus_tag="ACG86D_002057"
                     /pseudo
     CDS             14468..14980
                     /locus_tag="ACG86D_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(15378..15587)
                     /locus_tag="ACG86D_002058"
     CDS             complement(15378..15587)
                     /locus_tag="ACG86D_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN3141188.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5248 a         2662 c         3389 g         4407 t
ORIGIN      
        1 taaaataaaa ttgtcaaaac gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa
       61 gctgattgcg tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga
      121 tgtagagaaa acaaacgaat caacgcaatc agagaatcaa agttttgaca agactacttt
      181 ttcagaagaa tcgattactt ctgaaactac agaatctcaa agtgaggctt ctactaataa
      241 aaggcaagaa gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag
      301 cagtgaagcg gcactggcaa gagctgtttc agcggatggg tacagcgaag ctgccacaac
      361 agaagaactg gcgcagcttc tcgcagacga atcagtaact aagatccgac tgattcagcc
      421 tttgacttta gacagggaat tagagatcaa acgagatatc gttattgatt ttggtggatt
      481 cgctcacaat ttcggcacac accatattta tatcaatgag atacctcagt cgatcgagtt
      541 ccagaatttt aaaggaacag ctgcacatcc aggtggattg acaccaaata tcgatggaaa
      601 tgccattatc attgcttata tgaccgattt ttggggaagc agatatcatt ttacgggaga
      661 aataaaattt accgggacac ttgatttgta tgatgggtcc aaattaggac tgatttacgc
      721 tcctcgtgca actgtcacat tagatggtgt ttccggtgtg ttggatgtcc aaccagtgaa
      781 ggaaggactg aatgccgctc cgggtaaagc ctatttttgt agaagctatc aattgaatgt
      841 gatcaatggt agtcaacttt atggaccgta tctagggaaa ttctacggtt ttctagattc
      901 aggagatgag actggtagtg gaaaaacagc gccaggaatc catatcttaa gcggttcaaa
      961 ggtctctctt gattatgatc gggcagatgg cgttagcgat gaaggagagg cagtcgatac
     1021 gctacctggt aatgtcatgt ttgaagtcag tggttcggga agcgaatttt cggtaaatac
     1081 aaagatcaat attaccaatg acaacaatcg cggaatcatc caaatgagag gttcaggatc
     1141 gaaagtcact gtatcgaatg gtgggaaaat caccatcaat acagcaacta cgggcggatt
     1201 tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa
     1261 aatggctgga gatgacgatc aacccgggaa caatgggatg cgttttgtag gaaaaggcct
     1321 ttctttgatc gtagaaggag ccggatcagt tatcgacatc gataagcaga gtgggcgttc
     1381 cagcgcagtt cgttttgaga atggtacgca aaagctgaca gtaaccaatg gaggctcatt
     1441 gagggtaaga aatacaggtg atggcaaatc ttacgtgaac agactcaatc aaggaaatca
     1501 agcgatccaa ttttatgacg cttctggttt tctagaaagt ccaggttcag ctgattttac
     1561 agtcactgga gaaaaatcaa atatcgatat acgcgcagat tccggagcaa ctgtagatac
     1621 aactggagat attgatttaa attttacggc aggtgaaaaa acttatttag tcatggtcgg
     1681 gaaaacaggg gaagataaga ccggcatcat cagtggggat gtgttgaatg tggacttaga
     1741 atctcctacc tattttgatt tccaaaacaa acggattgga agcgagacca atggcatagg
     1801 cggctgggta ttccaaggaa atggagattc ttctttaaag atcgcccatt cagaaatcgc
     1861 tttatggcga aaaacaggcg caacattcga taactttgaa aaagacccgg attatttttc
     1921 tgatatgaca gacttatcga tcaatgggac cgacttaggc aatttcgtat caagccctag
     1981 tgcaggttta acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat
     2041 cagtgccaat aatcagctcc cgatgattga atccttaaga gtgccaacga atgcagataa
     2101 aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg
     2161 gacaggcgaa gtagaagtga ctttacgtat tacttacgca gatacgacga aatcatctgt
     2221 agaaattact gctctcacgc aaggacaaac agatgaaaat gaaggggcag gatataaccc
     2281 atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg atgagagatt
     2341 aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt
     2401 gacagaactg gactctgcca aaacgacagt cgatgtgatt cctcctgaac ctgctaattt
     2461 aagtaccaca ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg aagcaggagc
     2521 cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg atgatacagg
     2581 ggaatttgtt ctttctatcc cagaaaatca actttttgca ggagatgtat tagaaattgt
     2641 attaaacgac caagcaggag aagctactgc taagggtgtg cagaacaagc caagtaccaa
     2701 cgatgaaatt gggaatcaca atccaagtga tacgccagtt gtttatcatg atgccccgcc
     2761 ttttgcacct gctacgaaga tcgtagtaga aggtggtctc tcatttcttg ccccttccaa
     2821 actcgatttt ggtcagatca aagcaactgg tttgacacaa gaagcatttg gccaaatgga
     2881 tatacaggaa aaattatgga tttatgatca gcgagaaacg aaaaatccgt ggatcttaac
     2941 gataaaatta tctcagcctt tcacaaatga tgcaggattt tctatgcagg atgtcctata
     3001 tttcaagaaa ggagaggagt atcaattagt gaatgaagaa gaactggaaa ttgtcagagg
     3061 acaaaattca acgaatgtgc aagcagatta ttcagaatat ttaaataacg gtcgggcgtt
     3121 taaacttgtc ttaaccaaag aaaatcagat agctggtaat tactcagcag agataacatg
     3181 gacattagca gatgcgccga cagaataaag atcaaggagg atgattgatg aatcgaatat
     3241 ggaataaaag tcatcgaaac aactgctctt tacttatgat ccttctcctt ggattcatcc
     3301 tttggcagac acctgtccat gcctccgaat tcaattttgc ggtagatttt gaattaccgg
     3361 ctaatcagat tgataagaac aaaacatata taaatgtaag gatggagcct gggaaaacac
     3421 aggaaatccc ctacacggtc tttaaccgaa cagataaatc agtaacagtt gagacattga
     3481 tccactccgc tacgacgaat tcaaacggtg tgatcgaata tggagaatca aaaagtaaat
     3541 ttgatgaaac attaagttat gatatgaaaa acttagcaaa aacagtggga gaaataactg
     3601 ttcctcctca aagtgcggtc actgagacac tttcactgaa agcgccagaa aaagagctgg
     3661 ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa
     3721 gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt tgaaatagcg
     3781 aaaaagagtc ttccccagat ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa
     3841 gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc ttatatgttc ggcttgacta
     3901 catcggttga aatctacaaa aaagaaacaa aagagcctta cctttcaata aaaaaagaaa
     3961 aaatagatgt agcacccaat acaaattttg atttacttgt cccattagaa ggaaaaaaat
     4021 tagctccggg acattatcaa gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc
     4081 tccataccga ttttgaaata actgatgatg cagcggacca attgaatgaa aaggatattg
     4141 atttaaaaaa tcagccagaa acatttgact ggcgatggct gatacttgtt ggagtcattc
     4201 ttattggctt atttgctgga ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa
     4261 aacaattgca ttaagtaccg ttctagctgg aagtctatta ttatgtacac aaagtgtttt
     4321 tgctgagaat aagagctcag ccactacaga aggaacgatt tctgtgacca aaccagatgc
     4381 tgcaaatcct gaagacgttg ttccagttcc tcctgttgat ccaaatgatc ctgatactcc
     4441 ttttacacct gacgaaccaa atcagggaac atccggatta ctgacgattg accaagctcc
     4501 agatcttgat tttggaacaa tcaagttagg tacagcaaaa acagtatatg ctgcattaca
     4561 aagcggaacg gacagtactg gagcacaaac acaagtacag aatttcgtac aagtaacgga
     4621 taaaagcgga aattatgccg gttggaaatt atcagtcaaa cgaacagaat ttattggtac
     4681 aacatctgat acgaataaac taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg
     4741 aaatagcgca actaatagca gtggaatgcc atctaccgtt tctggaactg gcgctgaagg
     4801 aatcgaaatc ccagcagatc aggcagtaga tctagtaact gcaggagcaa atgaagggat
     4861 tggcacatgg atttattcat taggagagaa tgtggaacaa gggagacaat cagtggaatt
     4921 atttgttcct gtatccaatt atgctgtaga caactacgtt tcaacattca catggtcgtt
     4981 aacagatgct cctacagaag aggaagcgta aaatcatatc aacgatttca gggagtggag
     5041 ccgattcaag gcccactccc tgaaatctgc tatgcttacg tctcagctct tattcatcaa
     5101 ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa gatctttcga
     5161 ccaacgagta aggagtcacc gttgaaaaat tctaacacat aatgagcaga atcaattttc
     5221 tgcaattgcg ataagttgat acagtaagat tttaaaagta aaaagtaagc atacttttct
     5281 aactctgtct tcaattgttt taaagaagta ttcactaatt tatcttctgt agttgttttg
     5341 accataactt ggtttttcaa acctttgata ctttcaatat ataaaatttc ccgaggatac
     5401 aggacgagac ttccaccttt ttgatcaata aagattttat cgacatttac gatctgctgt
     5461 tctttccaat tgatctgttc tagcacttga cgaatttgtt cagataaatt cctgtctttt
     5521 ctgatatatc caatcggaag gattttccga ttgatgatct ctactgcaaa attcgtataa
     5581 gcggtgaaaa aaacaatatt actatgtgca ttttttttac gtatttgttc ggcgacatca
     5641 atacctgtga gtgattgctt caagtcgata tcgataaaga aaacatcgtg attatcaata
     5701 gtgcccaact ggatcaactg attgatttga gagatagaag tgagctgcac gatattctcg
     5761 atatgatgat tgaccaattc tctgatgatc cattcgctgt gaaactggtt atcttctatg
     5821 ataaaatact ttttcatatg caggctccta ttaaaaaaat atgtgtacat tttgaaacaa
     5881 aacaatggag gaataagatg gtcgcaacag tgacacttta tttgaccttt actttgattt
     5941 tagatttagg tatctattta ttcttttcga aacaactgct agtaaaaaac agctttggtt
     6001 gtatagcttt gatggtggtg acacttttac ttcctatccc taaagtgagc tttccttacc
     6061 tagttggctt attattgatg tttctttttt attttactac gaaaaacctg tttatcagct
     6121 gtttgatcag taatcttttt tttgctatca ctggactcag cttttttctg gtttttgatc
     6181 tgatggatta tttaggaaag agacatcttg gaacagcaag tttttttgca ctacttatcg
     6241 catttatttt ctatctatta gtaaaaagta tagacaaaca gacgagattt ctcaacttgc
     6301 tttttactta tccaactgaa cactattttg gaggtatttg tattttatta agctggggcg
     6361 tgatttactt ggtattggga agtgttcact tccgctcatc taattatctg cttctatctc
     6421 ttttgtcgat agtcatcagt tttttttctt tgtttttttc cattatgttg atcagtttcc
     6481 aagtaaaaga aagacaacat caggaatcgc ttcaactgta tcaaataaat gaagaatatt
     6541 accatcatct agaggaattc aaacatgatt ataaaagtct actgttttca ttaaaaatta
     6601 ctcttcagga aaataaaaaa gaaacgttgg aatttttaga aatattagaa gatcattctg
     6661 cagctatttt tgaaaaaccg caaatacaac agctaacaaa tattgtatcc ccagctgtca
     6721 ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg atccaagtag
     6781 ttattcctgt cgaagtgaaa acaatcccga ttgatctggt tgtttttatt cgctgtttat
     6841 ctatttttat ttctaacgca ttcgatcatc gtgtagccga tcagagtccg attacgatca
     6901 atctgctgga agaaaaagag ggatcacgtt ttagtatagc gaacaaggca aatccaacga
     6961 atttgacgct atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag
     7021 gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta aaaatcgaat
     7081 tttcaaaaca gaatcactat ttttttgtag aaatgtatct ggcatctaga aagagcaaat
     7141 cagcctactg acttactgtc aaaggataag tttgaggaaa gtctacacgg agatagagac
     7201 aacaacacag catattcaaa agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc
     7261 ttttgctatg tagttaaaca aagtagttga cacttccgct acttagtatt agtacgaaag
     7321 tatacttcat gatactaagt agataagaag tgaaaagcgg aacccacgat cgtcaaaatg
     7381 ctaactgctt tgcttacacc agataaaggg aagagcaata tcgcttattt taggcgtgga
     7441 gaagcaagca aatccctacg atcacaaaaa aaatgctgac ccagcagtta agagagttag
     7501 aacaagatga aagtatccat cgaaaagcct ataaccaagt cccgcctaaa gtagagtact
     7561 cgctcaccga agaaggaaaa tcattaagag agattctagt agcgttgtct gtttgggaag
     7621 aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga cacgctactg catacacatg
     7681 gtggctacct aaattattga ttttctatga catttatttt aaataaaaga aaacaggagt
     7741 ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg tatgattaat caagtaagaa
     7801 gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt ttagagatga
     7861 acgtaattgg cgacaatttc ataacgaaaa agatttggct atctctattt ctttggaagc
     7921 atctgaattg ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt
     7981 agaacgaatc aaagaagaac tggcagatgt attgatctat tcatacatga tggcagacaa
     8041 ccttcacctg gatctagatg agatcattga agaaaaattg atcaaaaata atttgaaata
     8101 tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat
     8161 tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga gagaaaatga
     8221 aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct cttgttcctc ttgttattag
     8281 taggactagc tttgattttc aatgaacaga tcaaggatta ttttgtcaga gagacaggag
     8341 ataaatatgc tatagcgaat gtaacaaaag aagatctgaa aaagaataac gataaagatg
     8401 tcagttttga ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa
     8461 tgagaggaac agatctacca gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt
     8521 taccggtttt caaaggcttg gataatacgt cactacttta cggagcagga acattatctc
     8581 cagatcaaga aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca acgaaccctg
     8641 aattgctgtt tacaccgcta gaaaatttag agatgggtgc caagatctat ttgacagatt
     8701 tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa agttgctcct acagataccc
     8761 aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg
     8821 acgccgtcac gcgactagtt gtacaaggag aactggaaag tgttacatcg ataaaagatg
     8881 cgacagatga tatgagatct gctttcaatc tagagacaaa aacattttag gatactggga
     8941 cataagtcgt tacagattaa aaacagccaa aggatatgaa tcgataagga aaaaccaatt
     9001 tgctgaaaaa aagagaaacg caaagaggtg catctctgat ttggttgttt tctagataga
     9061 cttgtgttcc agttttttta ttttttatat gaaaaggctt gcataaataa aaattcgatg
     9121 gtatgatgat tttgaataag aaatggattg ttactgtacg gcaggcaaaa ccagaattca
     9181 gctaagaaac agtaggcgaa ttctaggttt ttttattttt cttgagaggg cgaagcaaaa
     9241 tgaaaattga taaaattttg aataacaatg tcgtcatttc gaaaaatgga ttcggggaag
     9301 aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa gattggggac gagatttcgc
     9361 cagaagcggt ccaaaaagaa tttgtttaag agattctttt gctaaaaacc aattccagca
     9421 gctgatggct gatattccgg cagaggagat cgaatgggtc aaacaagtag tcgaactggc
     9481 tgaagataaa ctaaaagttg aattttcgcc taatatttat ctgacattga ccgatcatct
     9541 tcattatgcg atcacacgag caaaagaaaa tatccaactg cctaatccat tattatttga
     9601 aacaaaaaaa ttttatccga aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa
     9661 acaaaagacg ggtgtggaac tttctgagga tgaagccggt tttattgcct ttcattttgt
     9721 gaatagccaa caaggaaacg aagacatgca agtgacgatg accgctacca caatggtaag
     9781 agatatactg agtatcatca gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa
     9841 ttatcagcgt atcattactc atctgcagtt ttttgcccag cgttatctca aaggggaagg
     9901 atctgacgag caagatgaat ttctctacgc attagttcaa ggaaagtatc caaaagcgtt
     9961 tcattgtgtc gaacggatca atgagtatct tcgacaaacg caaaaacagg aaatggggat
    10021 cgcagagcag atttatttaa cgattcatat tcaaagagta gtaagtgaga aaaaaagcat
    10081 ccaatcataa ggattgttac tgtatggcag gcaaaacctg gattgaaaaa ataggttcac
    10141 gaatgtttgt gactgttttt ttcaatctgg gtttttattt tttaaataaa aaagaaagaa
    10201 ggaaaaaaca atggataatc aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca
    10261 aaagaatgtc aaaagtttag tacattgtgc tacgcggttg cgattcagat taaaagatga
    10321 atctttagca gatacgcaaa agttaaaaga agacccggac gtgatccaag tcgtacaaag
    10381 tggcggtcag tatcaagtag tgattggcag taatgtagca gatgtttatc aatcgatcgt
    10441 ggatgaggaa ggtcttgcag tttcggagga tggaaaagaa gagtccaaaa atattttgaa
    10501 tcgtctgatc gatattatct ctagtatatt tacacctttt ctaggggcga tggcagccgc
    10561 cggggtattg aaaggattct tatcattggc aacggtgatg ggctggctga cagctgatag
    10621 cggtacttat caaatattgt ttgctgcagc agatggtgtc tttaccttct tacctgtcat
    10681 gcttgctttt acagcagcta aaaagttcaa agccaaccag tttttagcag tagcaattgc
    10741 tatggcactt gtgtatccag ctatcacagc agttgctggt gcgggagaag ctatcagttt
    10801 ctttgggatc ccagtgattc tttcaccatc tggttatact tcttctgtca taccaatcat
    10861 tctagccgta tgggttcaaa gtaaattaga gccattcgta aaaaaagtga ttccgcaatt
    10921 tctgcaaatg attttagttc cgcttgtcgt tttagtagtg atggttccat taacatttct
    10981 tgctttagga ccaatcggga cggttgccgg taatgcttta ggcggtctct tcaactcgat
    11041 ttatggattt agtccaatcg tagcaggtct aatcatggga agtttatggc aagtattcgt
    11101 gatgttcggc atgcattggg gattcgtacc gatcatgttt ttgaatatcg aacaatatgg
    11161 gtttgatgta ttgatgccaa tgcttcttcc agccatcttg gcacaaggtg gggctgcttt
    11221 agctgtagca ttgcgaacaa aagatacgaa acttcgagca ttagggattt cttctacagt
    11281 gacttcgtta tttggaatca ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa
    11341 accattcatt gccgcttgta tttctggagg tattggtgga gctattatcg gattttccgg
    11401 agtaaaagct ttctccagca gtttggtcag cttattgacg attccgactt tcattaatac
    11461 ggtggatggt gtagagtcaa atgtgacagt agcagtaata gctacaggaa ttgcttttgt
    11521 tcttgcattc gtaggtacat tgattttagg atttgacgaa caaacacaag acaatcaatt
    11581 agaaaacaaa catgcgaatg caggagaacc gattacttca gcacggcata ccttaaaaag
    11641 tcctttaacg ggtaaagtac tgccattgag tgaagtacca gaccaagttt tttcttctgg
    11701 cgtgatggga aaaggtatcg ctattgatcc agaagtagga gaactagttg cacctgcaga
    11761 cggagagatc acgactattt ttccaactgg acatgctgta gggatcacaa cgactgacgg
    11821 agcggagatt ttgattcata tcgggatgga tacagtagaa ttaaatggga atggctttga
    11881 gatcttagtc aaacaagggg atctcgtcaa agcaggagat ttattgattc gttttgacat
    11941 cgaagcaatt agagcagctg gctatagcgt aatcactcca gttgtcatta cgaatactga
    12001 tgcatttgcc gatatacttg aattagacca aaaagagatt attgcaaacg aagatgttct
    12061 ggcaattgta aaataaggag gaaatcagga tgcaatcacg tttttcagaa aaatttttat
    12121 ggggtggagc aactgctgcc aaatcaatta gaaggggctt atgatcgaga cggaaaagga
    12181 ctatccgttg cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa
    12241 gaatttgact ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat
    12301 cattacgatc gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat
    12361 cgtttttcga ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa
    12421 gcgggattag aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct
    12481 gtgatcacga tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg
    12541 aaaaatcgaa aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac
    12601 agcagtaaag tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca
    12661 gctttaagtc aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa
    12721 gcatggcata atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt
    12781 tcggatatcc aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat
    12841 ccagtcaatc aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat
    12901 gtccaagtac gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc
    12961 gatccggaaa aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg
    13021 gactacatcg gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca
    13081 gcaacttcag aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa
    13141 tggggctggc agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt
    13201 taccagaaac cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag
    13261 gatggcacga ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg
    13321 gctaatgcag tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatcgat
    13381 cttgtcagtg catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta
    13441 gacgatgaag gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa
    13501 gaagtaattg cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac
    13561 acttctcaca tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata
    13621 gtaaagcaaa gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata
    13681 aaattaaaga agatattttc tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa
    13741 gtgaattaga agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc
    13801 tggcatcaga tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc
    13861 gtccttacaa tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat
    13921 atgaagtccg gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt
    13981 tgttttcctt tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc
    14041 agccttatat ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag
    14101 aatttggcaa agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat
    14161 tttctgatga tttctcggca gtatttttaa cagaagaaga acaaggaatt ctgaaaacag
    14221 acgcacagat cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg
    14281 aatattcgga ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat
    14341 aaataaaaaa agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    14401 aaaatagctt ctcatatttt tcgtttggtt aggcttatta ccgcagatac acggctgttt
    14461 ctggattacc atttattctg gataaggggg tgtggcacga ttttcagaag gaatctatga
    14521 accaatcact gtattgattt tagcttcgat ggtaacccca ttcggcttga cgatcgctta
    14581 tttcttaggg aaaatcattc gtaaaaatat actcaatcgt caagaaatcg atacattgaa
    14641 gaccgctttt cctatgggga tctgtcagat cacagaggga tgtttcccta tcgtattgaa
    14701 cgacttggca cgcaatgtca tagcaacggg agtcggtggg acagtaggtg gcggattaag
    14761 catgttttgg ggcgcagata gccgtattcc ggcatcggga atgtttgcag tcgctacgat
    14821 gaccagacca tgggcattca ttggtgcatt gttagctggt tcttttgtca caggaataac
    14881 cattttatta ttgaaaaaac cagtagatcc gaatgcagaa atcatccaag aagaaaaaga
    14941 ggaagaagac atatcttggg atgatttgac aattagttag gagaggcaga atggattaca
    15001 agaaaaaagt acaatagatg taccttgaga caggaatggt atgaaagaat cagagttaga
    15061 aagaatcgaa tatacagatt ttggtttagg agcgatcgaa actgtccaga gcatcggcac
    15121 cctccttgaa atgacgatga aggaaaaatg agaacctttc gttgtcgaaa gggtctggta
    15181 taccttatgt cgaaggaaag gaaacaaaaa aactttcctg tactattcca aaaaggaaag
    15241 aaagttatta tacagtaaaa aaagaaatta ttcttcatgc tagagatcag tacacgttcg
    15301 agcccaatat caaacactag ttccaggcag gcgcaacagg tgcggttatc actgaatttt
    15361 cttccagcag tgacgattca agtgatattt ttacaaatcc agctatttat cgttaacaaa
    15421 aagattttta ggagaagctg atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg
    15481 gcttttttat ttagagaaac atcatttatc agtgaaacat aacacgagaa caaaatgatt
    15541 ttttcgttaa taaaagaaac aagtatttgt tgtttaaaat aatacacgtt attggcatat
    15601 aggacgaaat cccagtatct tctataagta agtcatttca tatcgatgaa aacacgttat
    15661 ttatttttgt tttatctata aaatggctga aaaatgattt ttttct
//
LOCUS       DAZAJZ010000068        15420 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000068 DAZAJZ010000000
VERSION     DAZAJZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.068"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..455
                     /locus_tag="ACG86D_002059"
     CDS             105..455
                     /locus_tag="ACG86D_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN3141189.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            554..1084
                     /locus_tag="ACG86D_002060"
     CDS             554..1084
                     /locus_tag="ACG86D_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN3141190.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1238..2389
                     /locus_tag="ACG86D_002061"
     CDS             1238..2389
                     /locus_tag="ACG86D_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN3141191.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2498..4537)
                     /locus_tag="ACG86D_002062"
     CDS             complement(2498..4537)
                     /locus_tag="ACG86D_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN3141192.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(4595..5152)
                     /locus_tag="ACG86D_002063"
                     /pseudo
     CDS             complement(4595..5152)
                     /locus_tag="ACG86D_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5300..5466)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5691..5897)
                     /locus_tag="ACG86D_002064"
     CDS             complement(5691..5897)
                     /locus_tag="ACG86D_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141193.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5913..6527)
                     /locus_tag="ACG86D_002065"
     CDS             complement(5913..6527)
                     /locus_tag="ACG86D_002065"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN3141194.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6674..7357)
                     /locus_tag="ACG86D_002066"
     CDS             complement(6674..7357)
                     /locus_tag="ACG86D_002066"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN3141195.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7466..7924)
                     /locus_tag="ACG86D_002067"
     CDS             complement(7466..7924)
                     /locus_tag="ACG86D_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN3141196.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8008..9771)
                     /locus_tag="ACG86D_002068"
     CDS             complement(8008..9771)
                     /locus_tag="ACG86D_002068"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN3141197.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9992..11452)
                     /locus_tag="ACG86D_002069"
     CDS             complement(9992..11452)
                     /locus_tag="ACG86D_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141198.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11660..12586)
                     /gene="glsA"
                     /locus_tag="ACG86D_002070"
     CDS             complement(11660..12586)
                     /gene="glsA"
                     /locus_tag="ACG86D_002070"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN3141199.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12609..14036)
                     /locus_tag="ACG86D_002071"
     CDS             complement(12609..14036)
                     /locus_tag="ACG86D_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3141200.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14067..15281)
                     /locus_tag="ACG86D_002072"
     CDS             complement(14067..15281)
                     /locus_tag="ACG86D_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN3141201.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4398 a         3085 c         2657 g         5280 t
ORIGIN      
        1 ttttgtcaca atcctttttg tttttttgaa taatcgcttt ttcttctctt tttcttatat
       61 actataagta gtaaataatt tgaaaaaaag aaaggtgact gattatggcg caaaatcgta
      121 tttttacgac aagttttgca agcgtttatc ctatgtatgt agcaaaagcg gagagaaaag
      181 atcgtataaa agaggaagta gatgaaatca tttgttggct gacgggttat tcccaaacag
      241 aactagaata tcaactacaa aaacaagtga ctttcgaaca attttttgcg gaagcccctg
      301 tccccaatcc ttcacgttcc ttgattacag gtgttgtttg tggtgtacga gtagaagaca
      361 tcgaggataa cttgatgaaa gaaattcgtt atttagataa aatgatcgat gagctagcaa
      421 aaggcaaaaa aatggaaaag atcctcagaa gttgattttt ccagagatat tcttctcctt
      481 cgctcatttt gtaacattta atttggtaaa atgactagtt cacgttaaaa taagtcaaag
      541 gaggttttct tatatgtttg agacgattaa acaaaatatt caaaaatacg ccttattaag
      601 agctgctgtt tatattattt ctgggattgc gattgtgcta aacccttctg ctgtttttca
      661 ttttatcggt tatctgatca ccgcctatct tgttttgctt gggttgttga atctttttga
      721 ggcttaccgt tttaaaagga aaacaggttc ttggagtatc ggtctgttca gtggtctttt
      781 tttcctgatc ttagctctga tcgtctatgc ttttgcacct gcaatcgttt ctatcctgcc
      841 gattcttctt ggattagctg ttatcatcaa cagcttgttc cagttgttct tcagcatgaa
      901 tacaaaagct aaaagttgga gtgtttatag tagtattctg ttgttgattg gcggattcgt
      961 cttattgttc aatccattca aaagtttgat gcttctattc caaatattcg gctttatttt
     1021 gattttcatg ggcatccagg agattatcaa tttcatcaga ataagaaaaa tgactatacg
     1081 ttaatcattt ttagttatta aaatagaccg tataaaaacc aacaatccaa aaaaatcttt
     1141 tggattgttg gtttttttgt gagtatattt tcctattttt tcaataatgt atgttatgct
     1201 aagttcgggc tatattttat acaaaggaat gatgagaatg gaatttattg gtattttatg
     1261 cctgatattg atttcaacaa cgattggctc acatatctcg cggagattag gtatcccagc
     1321 agtgattgga caattacttg ttggtgtttt gctaggccaa gctggattga attgggtcaa
     1381 acctgatatt ctagtccatg acttttcgga gattggtgtg ttcttattaa tgtttttagc
     1441 tggtctggaa agtgatttat ctcttttgaa aaaatatttc cgacctggta tgttcgttgc
     1501 tttgcttggg atcatcttcc cggttgtttt tggctggttg acaggcaaag cattccaagt
     1561 ttcgggaaat gaagcgattt tctttgggat tattcttgcg gcaacttctg tcagtatttc
     1621 agtagaagta ttaaaagaac taaacgtagt gaatacaaaa gaaggatcaa caattttggg
     1681 tgcttctgtg gtagatgaca ttttagtcgt tttagttcta agtttcagcc tatcttttct
     1741 aacgggggaa gcttctagca atgcttcttt accgcttgtt ttgattgaac agctaattta
     1801 ttttgttttt attttcttat tagtcaaatg gattgcgcct ttcctacttt catttgcaga
     1861 aaaagtgtac gcaaattctt ctattatcat cgtatcgcta gttatttgtt taggaatgtc
     1921 ttacgtagcg gatcttgttg ggcttagctc tgtcattggt gctttttttg ctgggatagc
     1981 tgtcagtcag acaaaggtta aagaagaagt ataccacaat gtagaggctt taggatatgc
     2041 agtatttata ccagtatttt ttgtcagtgt aggattagaa gttgattttt ctgactttag
     2101 tgaacaacta ctctttattt ctatattgac gcttgtagcg attttgacca aattgcttgg
     2161 aggttatttt ggtgctcaga ttgccggttt ttctcaacat agttcattga tggtcggtgc
     2221 tggtatgatt tcgcgtggag aaatggcttt gatcatttta caaattggac agcaaagtca
     2281 attgatcgaa ccacagtact attcaccttt agttatcgtg attttattaa gtacacttat
     2341 ttctccactt gttctcaaat attttacaaa aaaagtttat gaagtgtgag taaatcaaaa
     2401 agaagttgtg aaagagacat cgtgaaaacc gtttatttga aaaaaggact gtgacgataa
     2461 cgccacagtc cttttactgt acttaaaaag caatttttta ttgataattt tggttgaggt
     2521 attgcaatag ttccgatgct cgtttggttg ctgaatcatc atcatcttta ttttccaaca
     2581 tgctgaccat catataacct tggttatcaa gattgaaagc aaacaagaaa ctgttctctt
     2641 gcccctttac atcctgtttt tctttgattt ccgctgtacc agttttcgct gccaacggaa
     2701 tccctaaagc agaaagagaa tgtgctgtac catttacatc ttgcacaact tctctcagat
     2761 ctggcacgat cgtttgtact gctgtttcgc tgatgacatt cttcttatct tttgtctctt
     2821 tatctgcaat caatttagga tagacaagtg tgccattgtt ggtaaaaaca gaatacattg
     2881 ctgcttgctg gataggagta attagaagtt cgccctgtcc atatccagta tcagctagca
     2941 agatatctga gttaaagcta tcttcattag aaatttgtgc tggattcata ctaattggca
     3001 aatcaaggtc ttcaccaaaa atgaatttat ctaagcctgt acgaaatttt ttctcaccca
     3061 ttttcaacgt ttcttgtgcc gcatatatat tatcggaata gatcaaagca gtttttaagt
     3121 ctacttgtga tacatcacta ctgacacgcg ttgttttaaa agatccccaa gaactgtctt
     3181 tttgccattt aagcccgttg atcgtcaaca cttcatttgg atcgatagtg ccgttgtcga
     3241 gaccgattgc tgctgtaatt attttaaacg tagatccagg agcataacct gtcgcaaatc
     3301 ggctgatgaa tggatgttca ggattttctt cataagcttt gtaatcttct tgtgagatcc
     3361 cgtttgtcat tttgtttgga tcatagcttg gagagctagc aagcgcaaga agatcaccgg
     3421 tttttggcgt tgtcgcaaca gttgatccag cttttcctcc tagactgtca aaagctgttt
     3481 tttgtgcctt cgcatcgatt gtcaatttga tatctttgcc attttgaact tcatgttcga
     3541 tcagaacttt tttctctaca ccgtctgcat ctgtaatgct taatttctca cctgttgtcc
     3601 cacgaagatc cttatcaaaa gccatctcca aacctgagcg accgattttt ccattactgc
     3661 ttaattctgg attcttatca atatcttctg ctgtgatatc tcccacgtaa ccaatcagct
     3721 gagcagctgc ttcacccaaa ggataatatc tgccgtctac ttcttgaatg gtagctccag
     3781 ctggaagttc tggcgttgct ccatcaatga ttttcaatgg gacaaagtaa tcgggttgta
     3841 cccagctttg tgaaatcgcc tgattgatag catcttctgt taagtcgaag gaggaagcaa
     3901 tcgctttgat attggctgtt ttttcgcctc catctccaag tttacttgga acgactccta
     3961 attgttttag tttgcctgtt gttgctaatg gttcaccatt tcgatctata atattccctc
     4021 ttgctgcttc ttgtgtggtc aggcttactt tgtcatttcc ttccatttct ggaaaaacca
     4081 agttgggttg ccagttgatc gtggtttgac catcatttct gtctaatgtt cctttataag
     4141 aaagatcttt caattcacct aatgaggtat tcatctttgc tttatatgaa aaactgtaag
     4201 tagaatcgtc ttttttatct acttttagat tcgatatctg aagtcctttg acatcagcag
     4261 caccgtatat attctggtat tgatctaaga tctctttttc agataatgca cttttatttg
     4321 ccgccttttt tgatgtcatt tctgcagctt tgttataatc tcccttgttc aaagcttgga
     4381 caaattgctc aactgtcttt tctccagctt ctactgcttg ggtttcttga tagtgctggt
     4441 agtaaaaata accgccaccc gcagctgcta ttaagatcac tgcgccggca atataagcgc
     4501 ctgttcgatt tttgccgtgc ttgtcacttc ttttcatttt ttcctccttc tcttgcttgt
     4561 cattccttta tttcactatt tatacctgtt tattctaaca tatgtctttg aggaaatcca
     4621 taccacaatc cagaagcaca ctttctggaa ggttcgtttg gatactacca agtaatttcc
     4681 caaccgcttt gggtagcttg aatattgaag ttacgtcttt cagctgctga gagatagctt
     4741 tgatcacttg ctgctgcctt ctgatcctcc cgaagtctcc ttctttgtct tcccgaaatc
     4801 gagcatactg gagtaaagta ttaccatcca tcacttgctc tcctttgttt atatatacgc
     4861 catctaaatc tagagatttt tctgcatcga tcttgactcc accaggaaac agttcattaa
     4921 cgcaatcgat aaaatcttga aatgtgatac ttgcataata gggagcttca aatttgaaat
     4981 tttcctttaa tgtttgattt aataaatcaa ctcccccata agagtaagct gcatttatct
     5041 tattcattcc gtaacctgga atatcgacat atgaatctct catgatagag atcagttttg
     5101 cttgttgtgt tttctggttg tattgtgcaa ccagaagtga atccgagcga cctccaccaa
     5161 tcgatctttt ttcaccgcat ctcctcctca cacagcaatc cacttgtcag gtgaggagcg
     5221 agtttgtctc atcaaataag tcagtcacaa aaatataaag aaaaggtcag aaaccttttc
     5281 gaacaagaat tctgacctag catcatctca tcgtccgagt tttagcacta tacaacgtaa
     5341 agaaaacatt cttagctatt ggaaaaagcc ttaatccaac agtttctgtt ttacccattg
     5401 gcgactttcg acgtttctgg gcaacagcct atgtttgtat aggagcctca cctaacaagg
     5461 ttattactat attctattta cgagttcatg atagactttt cctaatgatt aggcaatgga
     5521 atttgaaatg aaatagtgaa atttaacaat ctttaatatt ttttagaaaa aaataacaaa
     5581 taagttgaaa gaatcccgtt gcaaattctt tcagttgcac caggattctt tgataatttt
     5641 ttagttttat gtgttttttt atatgttttt tcacttttcg gaataaatat ttatttgtta
     5701 aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt accatctaaa
     5761 gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta ctgcccttgc
     5821 agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt cgatggttta
     5881 tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat cgtgaaaatt
     5941 tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa ttttcttgga
     6001 tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt ctgtacttct
     6061 agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg ttcatataag
     6121 gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat tactgaatca
     6181 gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt ggctgctgtt
     6241 ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc tcttgtctca
     6301 agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc caaggataag
     6361 atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt cactatatct
     6421 tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc aatcagcaac
     6481 ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt ttcgccttct
     6541 ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa tgcttttttc
     6601 atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca agaatctgtg
     6661 ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt ttagtgatca
     6721 attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat tcatatgctt
     6781 tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata tcgacttgtt
     6841 ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa tattctgtat
     6901 agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga agcgcttcct
     6961 caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt tggacaagtt
     7021 cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca atgatttctg
     7081 cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac acttctgacc
     7141 catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc ttgatttcat
     7201 tgatatcttc tgtcgtattt gctcgaatac caactgctcc gccttctttt gcagccaaag
     7261 ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct tgacaagaaa
     7321 cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca gtaatgttat
     7381 ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagac aataatgagc
     7441 tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct tcgtatacga
     7501 ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt ttaaaaaact
     7561 cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct ttccaagcat
     7621 gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg atgatacaaa
     7681 ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt aaaggctcat
     7741 tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt tttacaacta
     7801 gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa cctaattttt
     7861 ctgattgata actaggatat ttttcttgga actttttgac atattcttct acttgctgat
     7921 acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac tagtataaac
     7981 aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc
     8041 aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc
     8101 cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg
     8161 actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc
     8221 gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt
     8281 gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc
     8341 aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc
     8401 tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg
     8461 gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc
     8521 ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa
     8581 tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac
     8641 tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt
     8701 acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat
     8761 agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt
     8821 tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt
     8881 ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac
     8941 ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt
     9001 atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg
     9061 atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc
     9121 cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt
     9181 ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc
     9241 attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc
     9301 aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc
     9361 tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg
     9421 ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg
     9481 gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg
     9541 atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat
     9601 cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg
     9661 caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt
     9721 gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc
     9781 tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat ctattctact
     9841 acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata
     9901 taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag
     9961 ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa
    10021 gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct
    10081 ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt
    10141 ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta
    10201 tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga
    10261 ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga
    10321 tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt
    10381 gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt
    10441 tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac
    10501 ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga
    10561 tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat
    10621 aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt
    10681 tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct
    10741 cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt
    10801 cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt cctcgtatga
    10861 taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa
    10921 tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga
    10981 cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt
    11041 tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata
    11101 ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa
    11161 aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag
    11221 aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt
    11281 ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag
    11341 tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt
    11401 cacacagata cacttttcta aatatatttc gctcgatgta attttccagc attcctgata
    11461 cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt
    11521 cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc
    11581 aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc
    11641 cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt
    11701 tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac
    11761 ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag
    11821 gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa
    11881 ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa
    11941 ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg
    12001 agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac
    12061 ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga
    12121 tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc
    12181 gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg
    12241 cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat
    12301 tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt
    12361 ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat
    12421 cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt
    12481 gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat
    12541 agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct
    12601 ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga
    12661 ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca
    12721 gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga
    12781 cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca
    12841 agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc
    12901 aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg
    12961 cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga
    13021 gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct
    13081 gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct
    13141 aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt
    13201 aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact
    13261 gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca
    13321 aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg actggcagat
    13381 gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc
    13441 gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg
    13501 acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa
    13561 ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc
    13621 cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata
    13681 cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt
    13741 atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg
    13801 acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac
    13861 aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag
    13921 cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc
    13981 aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat
    14041 cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc
    14101 aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt
    14161 aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga
    14221 atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc
    14281 cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca
    14341 tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg
    14401 atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc
    14461 aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt
    14521 gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca
    14581 ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac
    14641 caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct
    14701 aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc
    14761 gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca
    14821 ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata
    14881 atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc
    14941 acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac
    15001 atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca
    15061 ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt
    15121 atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat
    15181 ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc
    15241 atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta
    15301 cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac
    15361 ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt
//
LOCUS       DAZAJZ010000069        15286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000069 DAZAJZ010000000
VERSION     DAZAJZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.069"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    complement(264..497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(574..1245)
                     /locus_tag="ACG86D_002073"
     CDS             complement(574..1245)
                     /locus_tag="ACG86D_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3141202.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(1332..3980)
                     /gene="mgtA"
                     /locus_tag="ACG86D_002074"
     CDS             complement(1332..3980)
                     /gene="mgtA"
                     /locus_tag="ACG86D_002074"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN3141203.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(4322..4496)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            4669..5106
                     /gene="ndk"
                     /locus_tag="ACG86D_002075"
     CDS             4669..5106
                     /gene="ndk"
                     /locus_tag="ACG86D_002075"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN3141204.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(5128..5994)
                     /locus_tag="ACG86D_002076"
     CDS             complement(5128..5994)
                     /locus_tag="ACG86D_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN3141205.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(6154..8247)
                     /locus_tag="ACG86D_002077"
     CDS             complement(6154..8247)
                     /locus_tag="ACG86D_002077"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3141206.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(8996..9619)
                     /locus_tag="ACG86D_002078"
     CDS             complement(8996..9619)
                     /locus_tag="ACG86D_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141207.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(9778..11175)
                     /locus_tag="ACG86D_002079"
     CDS             complement(9778..11175)
                     /locus_tag="ACG86D_002079"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3141208.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(11207..12178)
                     /locus_tag="ACG86D_002080"
     CDS             complement(11207..12178)
                     /locus_tag="ACG86D_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3141209.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            12552..12977
                     /locus_tag="ACG86D_002081"
     CDS             12552..12977
                     /locus_tag="ACG86D_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141210.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(13465..14166)
                     /locus_tag="ACG86D_002082"
     CDS             complement(13465..14166)
                     /locus_tag="ACG86D_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3141211.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(14182..14622)
                     /locus_tag="ACG86D_002083"
     CDS             complement(14182..14622)
                     /locus_tag="ACG86D_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN3141212.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(14622..15197)
                     /locus_tag="ACG86D_002084"
     CDS             complement(14622..15197)
                     /locus_tag="ACG86D_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN3141213.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         4398 a         3220 c         2532 g         5136 t
ORIGIN      
        1 cgtgttacca cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat
       61 actctagcaa gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg
      121 ctcgatggcc attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt
      181 caaaggttct tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaagtcct
      241 tttactaaca taaactatgt cagtaaaagg acgacgtatc gcggtaccac cttttttcgg
      301 aagtttcctt ccctcaagcc caacatctca aaaagagaga ttggcgttcg ttttaacggt
      361 cgaacagttc cgaatttgcc tacttatcga caaattattc tccaaggcca ttttcagctt
      421 ttcttacttg actcttctca ccagccagag cctctctgca aagtttccaa acttactttc
      481 cttttcaacg aaatcgatat tttgttgttg ggattaatct acatgaggaa aactgaaaag
      541 tcaagtacga aattcaaatt attttttcta tatttatttt tctgtttttt tatttaacat
      601 ttcaataaca gttcgaagat cttttccttc cgcaagaagt ttcaattttt ccttttcttc
      661 gacggtataa tttcggacaa aactggaatc aggtatcaaa acaacgccta atcctgctcg
      721 tgaagcagct gttgctccta ctaaagaatc ttccactgca aaggctgaac tagcttctaa
      781 tcctgttcgt tccaaagctg tcagataagg aatcggtgaa ggcttgttct ccgacacttc
      841 gtctccgaat gtaaacgtgt ccaagtaatc agctaattta aagtgatcca agatatctgt
      901 tgctcttttc gtaacagtgg tcgtagcaat gcctagtttc atatgacgtt cttttaagtc
      961 tgccaatatt tcttttgcat atggttttaa gtggatggct ccgcttgtca gctgttgata
     1021 aaaatattct tcacgcttcg ctcggacttc ctgtactttt tcagggctac ctgctgcttt
     1081 cactaaaata tcaaaacttt gtttccagtt ttttccgccc caagcgtcaa cgatttcttt
     1141 tgggatatgg atatcaaatt gctgaaatcc ccaaagccag ccatcacgat acgttttttc
     1201 tgtgtcaact aacaaaccat ctaaatcaaa tattgcacct ttcatctata cattaatcct
     1261 ttccaaaaca atacacatag tttagcacga ctggtccaaa aagaacagaa gcaaagaacc
     1321 agccttctcg tttataagag ttcttggaat cgacggatat agattttctt cacagctgtc
     1381 acaagcgcca gataagcgat gactgtagca aataaccata accagtaatt gccaggaagt
     1441 ggcatcaagc ctaattcagc accaaaactt gtaaaaggaa ggacagaacc gacaataatt
     1501 cctaatgtag tgataacaaa catggcaaat gttgcgttgc tttggataaa tggtactttc
     1561 ggtgtacgca gtgcatggag cactaaggtt tgtgtccata gtgattcgac aaaccatcct
     1621 gcatggaata atgcgatgaa aatcactcgc tgtgaactat ccaatgtatg gaaacttccg
     1681 cccactacag caggacaaat aacaaaatac atcaacaagt aagtagtaat atcaaatatc
     1741 gaacttgttg gaccaaagta aaccatgaat tccccaatac tagatgcatt ccattttttt
     1801 ggctcgtcca aatattcttt atccatccga tcccatggca aggaaatgca ggaaacatca
     1861 tagatcagat tcaaaaacaa aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta
     1921 cttgcaatca gcacggaaaa catattgcca aaattagaac ttgctgtagc cttgatatat
     1981 ttcatgatat tcccaaaagt ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa
     2041 tctttttcta aaaggatcac gtctgcagat tctttcgcaa tatccacagc tgtatctaca
     2101 gaaataccga tatcagccac tttcattgca ggagcgtcat tgataccatc tcctaaaaat
     2161 ccgacagtat gaccattttt acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc
     2221 aattttacga atacttcatg gtcttcagct acttgtgcta attcatgatc actcatttcc
     2281 gagattttct caccggtaac caattcattg atctctagac ctacttgacg gcacactgaa
     2341 cgtgtaacta attcgttgtc cccagtcaga acttttacag ctacaccatg ttctttcaga
     2401 gctttcaacg cttgtttcgt tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc
     2461 aaaaccatgt ctgactcatc tttgactgaa aattctccaa cgacgcttgg attggtcttt
     2521 tgtgcgacac caatcactcg taagccatct tcattaagtg actgtacttt tgcaagtacg
     2581 gcttcttttc gttcttttgt taatggtgta acagtaccgc caagatcaat aaatttagaa
     2641 atgttcagca tttcttcaat cgcgcctttt gtgatcattt gtgttttacc agctggatca
     2701 gcaaccacaa cactcatacg acgacgttca aaatcaaaag gaatttcatc cactttttga
     2761 taattgatct tatctgtatc taaagtttct gttgcttcat caatgatagc cacatctaac
     2821 aaatttttca gccctgtttg atagtagcta tttagatacg catggcgcaa aacacgatca
     2881 tcttcatttc cttcacaatc catatgatat tcaagaatga ttttatcttg tgtcaatgtc
     2941 cccgttttat ccgtacataa cacatcgatt gcaccaaagt tctggatggc attcaaattt
     3001 ttgatcactg ttcctttttt ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg
     3061 atcattggaa gcatttcagg tgttagacca actgctacag atagtccaaa aagaaaagct
     3121 tctagccaat ccccttttgt aagcccattg atgacgataa ctgctggagc catcaatgac
     3181 ataaatttga tcagtagcat ggatgtttta tggatgccta attcaaagct tgattgaatt
     3241 ggtttttcag atagcgtttc tgctacatga ccaaatagtg tttcatttcc aacagaaaca
     3301 atgattcctt ccgcacttcc actgatgaca ttacttccca taaagacaag attttcgtaa
     3361 cttgtttcgc tttgctgatc gtcttcacat actttcgcat gcttttctac tggatagctt
     3421 tcccctgtca atgctgcttg ggaaatgaat aaatcctttg cggacaataa acgcatatcc
     3481 gctggtatca tatcccctgc tgaaagccga acaagatctc cacagacgat ttcttccata
     3541 ggaatctctg tgtactttcc attacgtttt acagctgccg ttactttcac aagtgatttc
     3601 agtttttcag ccgcttgatt ggaacgtacg gactgaatca acgtcattgt cccgctgacg
     3661 ataaccatag tgaagataat ggctacaccg aaaagatctt tatctccagg agctgctaat
     3721 acatattccg tgatgaatga aataattcct aatgcgatca acactaatgt aaatggcgtg
     3781 atgtatgctt tcaatatttc tacaatgaat ggtgtttttt tgccatatga aatcgcattt
     3841 tcaccgtaat tttctcttga acgttcgatt tgttcttctg tcaatccatt tgctgatgca
     3901 tggaatagtt caaataattc ttgtttcgtt tttccagcaa aagctgcata gattttttgt
     3961 gtatctttct ttttttccat ctcaatctct cctttgtgtc caaaagacag ctgttctcgc
     4021 tgcttttggt ctttagctat tcatcggatc gatagagaaa aaactgaaag gggctgagaa
     4081 cagatagata atagccctgg cctttcctct agatccttgt ttttcgaatg actgtcatcc
     4141 atttgttctc atctccttta ctaagtaatt ttaattcatg acttccaatc atttttcgaa
     4201 tagttatttt tccaattaga ccacctcctt tttatttttc accgatgggg atttgcggca
     4261 acaaaaaaac ctctcgccaa atactcagcg aaaggtttga aaaagcatat taaaaaaagc
     4321 ttctcagatc tcatcgttga gttttgacac tgtacaacgt aaaaagcaga tacctgcttt
     4381 tagctacctt atgaaaagcc ttatctcgac aattcctgtt ctacccattg gcgtctctcg
     4441 acgtttttgg gcagtagcct atgtttgtat aggagcctca cctaacaaat cttattcggt
     4501 tttccttcgt taatataaca actaaaaaag aggatgtcaa atcaattttc catgtttttc
     4561 atactttttt taaaatacac acgttatatt cctgtctcac gcggttttat ttgtctcatt
     4621 ttccacctcc cgctataata gaaatatagt aggaaacgag gtgctgttat ggaaagaaca
     4681 cttgttatta tcaagccaga cggcgtgaga cgccatttag tcggaagcat catccaacgt
     4741 tttgaagcaa agggactggc cattgctgaa atgaagtttg aaactatgac tcctgaatta
     4801 gcaaaagaac attatcagca cttgactgag cgcagttttt ttgatgaact gatcgattac
     4861 atgacttccg gtcctgtcgt ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc
     4921 cgtaagatgg ttggagcaac aaaagctgcc gatgctgttc caggtacgat ccgaggagac
     4981 tacgcgctgc cggggactga aaacatcatt cacgcttctg attcccgtga tgctgcggta
     5041 aaagagatcg cccgcttttt cccgccattc gatataaaca aaccgtctga aaaagcggcg
     5101 aagtgataca aattctaaac tgctggtcta gttgctgggt tcagcaatgg ccagcagttt
     5161 tttctctaag gacctccgtt gattctttcc taacatatat tcgagcactg tgaagaattc
     5221 ttggtagtct ttcgtctttt cttcccctaa agcagcagaa atcaatcttt gataataatc
     5281 tgccatcaat gtttcataga tcactgtact tctttcttta tgtgataagc tccgtttcat
     5341 atcttcattc acactacgtg cctttttcaa ccgaccattt tctatataaa acgaagtcag
     5401 gttcattaaa aaagtaaaga gatactgctc atgattgttc aactgtgcta atcgtcgcat
     5461 tctttttttg attttcaaat aattgttctc aatgaaccga tcactgaata tccaaggaaa
     5521 atcagctaga atagatagtt caaattgacc atagtctggt atctgcatca agtattcttc
     5581 aatgattcgg cattcataac gtaaaaattc aaattctgtc tcagatactt ctgttttttc
     5641 gaccatgtga agattcaaaa ccaaataata cagatcttct gttttcttaa aatgcctacg
     5701 gttattgctg atctctttat aaggatcaaa gctgtcttct tgtacccgat ctagcattcg
     5761 tctttttttc tttaacttta ttggttcatc cagctggttt cgcatccatt catattctgc
     5821 aagtgtcatg ttcattcgct ctaaaaatga tctcaatgtt acaaatgaca tttttttcac
     5881 tgaatttgtc tcaatcccga ctagagaagt cttaaaacag attccttccg ctaattggga
     5941 ctgtgtcaac ttcttgttga ctcgaagttt cttgatcaat tcaccatcat acataatagt
     6001 tctccttttt aatttaaatt tttccacatt caataaatag atacgttaaa aaggaaaaat
     6061 acacgaaaaa aataaaaaaa tgtttttttc ataaaaaata ccaaccttca agcattagaa
     6121 tgttgtctaa ctactgaagg ttggtttgat tgtttattga ttattttttt gctgttcaac
     6181 gtctgttctt gttactggat ataagttggt tgttgtttta ttaccatttt ttgaaaagac
     6241 acttgtcgaa tgttcgcctt tttgcttttc gattgcttca ttacgttccg cttcattttt
     6301 atagtcatat tttttcggat caatcgtctc aaatccgttt ggcacataaa accgcaagag
     6361 attttcctga ttcagcttat ctgaaagact taacgcagtc ttgacttttt cctgatcttt
     6421 ggagatttcc tcatcgattc ctgctgcttt cgtatcgatg atttctcctg tctgattatt
     6481 gtaagctttt cctcctagga cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc
     6541 aactacttga tcatgctgag aagataataa gtctgtacca aaatgaagat aatctttatc
     6601 atcgattccc aatagatgta gtaatgtcgg caaaacatcg atttctccac cgtattcatg
     6661 attgactgtc cctttagtat aaccaggcat atggatcatc aatggcactc gttgcatctg
     6721 tgcgttgtca aattcatccc atgtatctgg atctttacca agagcgcttg ctaaatcttt
     6781 attatctgta ttcgagatcc caaaatgatc cccgtaaatc acaaagattg aattttggta
     6841 aataccagat tttttcaaat acgtaaaaaa ttgttccaat gattgatcca gataatgtgc
     6901 cgttcgaaca taattatcta ctgtgctatt gccagtattc aaagatggaa agtcgaaatt
     6961 cttgtcatcc gtataataag gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg
     7021 ctgttgtaga tgctcaagat attgggcaga ttctctgaat aaatctttat ctaatatccc
     7081 ataacctaac gtttcgtcgg attcatcaaa ataagatcga tcaaagaaat tgtcataacc
     7141 taagttttta tatacatgat ctctattcca aaaactagct acattcccat ggaaaacagc
     7201 gcttgtatag ccttgctttt gtccaaggat cgctggtgcg gcttgaaaga cattgtctga
     7261 cccaagttct gtgaataatg agccttgagg taaaccaaat gtgccagttt ccaacatatt
     7321 ttcagcatca cttgtctttc cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt
     7381 tgcttgattc tggaaaatac tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat
     7441 attaatcaaa aactgctgga aactttctaa atggagaacg atcacatttt tccctttagc
     7501 gatcccaaat gtttcgggat ttggctctgc ataatgtttc tttgtataat ctaaaacttc
     7561 atcaatccca ttactgcttg catgcgcgcg aacactgcta gtcattcctg ttttgattcc
     7621 gtcataaata gtatacgcat cgattcctag atatttgaca atatatgaac ggtcaaatgt
     7681 tcgctgcagc aattgtggac ggttggcttc gctcagcgct agatttcctg aaaaaataag
     7741 ccctgaaaga caggtaacag cgatagccat cggtttgccg acagggttgc ttttgatagg
     7801 tattttcttg accttcaacc aaaccagtag cccaatgaac acagcgatat ccagccagta
     7861 gatgacatca tggggcttca tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt
     7921 cgaaaagcct aatacagatt tgactgtaat gaaatctgtg aattcccgat aaaaaataat
     7981 attcaagtac aacaaaagcg tatttaaaat atcaatgatc aaaagaatag ctaacgctgg
     8041 ctttggcttt ttaatataca aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt
     8101 gatccacaag ataaagtact ggatcgtacc ttccacacct aatgaaaaat ctgcatagta
     8161 ggcaatgatc gtcttcagcc agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt
     8221 ttggcacaat tttttaagtt gtgtcacgta tatcacttct ctctattcac attttttctt
     8281 attttgctcc tgaaaaatga aaatttccta aaaaagttgt tatcttaaga tttttttatt
     8341 ctttctaaag attattccgt ccacttttca cagcataaaa ccgctgcgat gtaagcgatt
     8401 aaactaatga agagataaaa gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc
     8461 agtttctcgg tattcgtaca aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta
     8521 tttcttgatg caggacagct ttttcacaac ttcttcctta aacggtgttc tcctgccaac
     8581 tcctcggcca caagtcacaa tatttataaa acatgagaag ctgttttttg aaattgttcc
     8641 ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct
     8701 gaccctcggt attagattat gtcaggacgc taattttaca acctcttggt aaacggtgtt
     8761 caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg agctattttt
     8821 tgaaattgct ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt
     8881 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
     8941 gccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct gtttttcatt
     9001 gtccatagga ttgatattgt ccttgtgatg actcattcgt aataattgag gcttgatttt
     9061 cttcacttcc cgttatcgtt tgtccactgc cagaatctga aggacttgtt cctgtcgact
     9121 cgtttttttg agaagagaca gttgttttct tgacttgttc atttgtcttt ttccattccg
     9181 ctaaacgatc ggcgaccact tttttgtcta ctttttcaga ataagcggtg atcacttttc
     9241 catttttcaa acgggctact ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct
     9301 tgtctttaaa ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg atattaataa
     9361 attgattttc ttcttttcct tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg
     9421 cagttatttt atactccgcc caccaataag ttgagaattc ttttttgaaa ttatctactg
     9481 attctttgct tgctacgatt ttttctgttt ctttctgagc aagatctgct gtcatttttc
     9541 ctgaaaatgc tgcggataaa ataagcagta agaaaaaacc gaataaccaa aagagaagaa
     9601 acggtctttt tttcctcatg ctttcctcca ctcctattta attttgacta ttctattata
     9661 tacaaaaaat gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct
     9721 ttttcacaaa atggtaagaa tggagaattt cacgcatttg ctctcaacta ttctttctta
     9781 agcctcaaat ccgtttttat ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt
     9841 tctttttcca tcttgtgcta agtctactga aataaagcca tatcgattct tatatgcatt
     9901 ggtccacgac cagttatcca tacatgtcca catatgatag cctaaacaat tggcaccttc
     9961 ttgaatggct tgatggacat atttcagatg agattggaca aattcgatcc gataatcatc
    10021 gtggatcatg ccatcttctt tgataaatcg tgcttcgttt tctacaccca taccgttttc
    10081 agaaataaag cagtcgatat ttccataatg atctcgtaca ttgatcaaaa tatcatagat
    10141 tccttgttca tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata
    10201 attgtcgaaa taatcctcag gcattggacc gtttttagta tcgattggtg tttcttttgc
    10261 ttttacccgg cgtggctggt aataattgat acctaaaaga tctactgtat tttctttgat
    10321 aaccgtcaaa tcctctgcat ggatcacagg catgagatca agttctttta ctatctcaat
    10381 caattcactt ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc
    10441 atctgctatt tgcgctgctt taacatcttc agggtttgat tcgtctcttg gatagcttgg
    10501 tgtcagattc aagatgatac caataacacc accttgctcc atttcatggt aagatttgat
    10561 ggctaacgct gaagccaacg cttcatgata gccaacttgg atcgcttttt tcatatctga
    10621 ctcttctgga taatgccatc gatataaata acctgcttcg actggaacga ttggttcatt
    10681 atgagtaaac cattttttta ctttcgaacc aaacaattca aaacaacttt tggcatattt
    10741 ttcataggct tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg
    10801 catatcgaaa tgatacagat tcacaaaggg ttcgataccg ttttcaatca actcatcaaa
    10861 ataacgatga tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa
    10921 acggctccat tggatagatg ttcgaaatga attatgccca gtctgtttca tcaactgtac
    10981 atcctcttga tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa
    11041 cttttgcggt tcttttttgt accaataatc aaaaatgttc tctccttttc catcgcctga
    11101 aatccttcct tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa
    11161 ttgatatttt ttcatcaaag cacctctttt tcttttttag ttcgttttag ttgattccat
    11221 gtatgatatt ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt
    11281 ttaacagctg ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt
    11341 tcggaaggat actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct
    11401 tcccgattct gacaccttct tgattgaaac attcgatggc accaatcgac atattgtaat
    11461 tgctgacaaa aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc
    11521 tttttctaga atagtctcct gtaaagacct ctaattggat cgatggatca ttttggaccg
    11581 cttctttggc tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa
    11641 atccgatttt tttatgtcct tgttcaccat gatgagcgat cacttttgct acactttctc
    11701 gatcattcac taatattgta tcaatacctg ctggtccttc tggacaattc aatgcgataa
    11761 ctggaatact catttttgtg agcatttcaa atccagacca ctcttcttcg gataagaaga
    11821 caatcagccc atcgatcaca tgcttcatca ggtattcaat tttttgttca attttttctg
    11881 gctgatcggc aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga
    11941 tccctgagac aacttccgac ccaaaacggc tggaaatccc gtttatcaaa agtccaatag
    12001 agaagcttcg attattcttc aaccctttag caatgatgtt ttctttatag tccaacgctg
    12061 aaatcgcttc tgctattttt ttcatgttct tttccttcag ttgctgacca ttcaagtaac
    12121 gggaaactgt tcccactgaa acaccagcca tcttagccac atcacggatc gatgccatac
    12181 attctcactc ctgccagtta catttgattt ctctttcttg ttgttcgatt atgatgaccg
    12241 cattctttgc atagtgttgc ttgtcatcaa tatccatata actaaagtct tcagtagagc
    12301 aacttcctct cactattaat tatacttaaa caagtatttg aaccggttca tttttaatat
    12361 agcatttttt taaaagattg caacactttt ttaaaacgct ttctgaaagt ggcggatttt
    12421 ttgccttttt aaaattttat tctaaaattt actctatttg tgggtgtttt gtgcgttttt
    12481 ctcttccatc cgtttgaagt tttacattcc aatggtataa tttctttatc ctatcaaatg
    12541 ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat
    12601 tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt
    12661 ctgtcttaaa agcggaaaaa gcagatacca ccaacagtgt atatacgcta aaagtacgta
    12721 aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac
    12781 gcacgtatca gacacaagga actgattctt ctgaatcatc tgaagaaaaa acagtgctga
    12841 cagatatcaa gatcactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa
    12901 aagaaacaac agtcatattt acaaaaatcg gtaattaccg tttgaaggat gaggatggca
    12961 acatctacag tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg
    13021 ctatagtaaa gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat
    13081 tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg
    13141 agcatatgcg acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat
    13201 cctgttcaaa attatgtaaa aagtagaaga agataaacaa atccaagcaa aactcactat
    13261 ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc atcagtaaaa
    13321 caaacagtgg atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat
    13381 ttgaatataa aataaccaaa cgatagaaag gcttattcta catcacgtag taatttttct
    13441 ttcccgtcgt ttggtttttt atttttagcc taattcttga ccttttgcat gctggttatc
    13501 cgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt ggatctgacg
    13561 gatcaccttt tttgcatctt ctggattttc tacaaaatcg aatttatccc catccacttg
    13621 tattttagga catacatcaa aatcttcata ccattgatta tagcgggtgt tcaacgtttc
    13681 ataataggta tataacgtct catcgctttc tagctgttca tagtcacgcc cacgtttttt
    13741 gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat caggccgttt
    13801 ttttggagca acatcatcta actcgtttag catcgtatct aataagctat cgtactgctg
    13861 tacttcgata tccgttaccc gacctaaatc agcgtttaga tggaacaaca ggctatcttc
    13921 atagatggat cggtctagca cattgtcatc gttcactaaa gcatttttca ttgctaaaaa
    13981 tcgcttgttc aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa
    14041 gagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat caatctcttt
    14101 tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac
    14161 tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa caggcaatgt
    14221 ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtatgga gactttgtgt
    14281 aatcatcaag ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat
    14341 agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct caaagtcaag
    14401 gatagcaaca acaaattctg catttgtcag ttcttccata tctttttcgt atacttcctg
    14461 cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg gagagtagaa
    14521 acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc gatcgatttg
    14581 ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc ctccttcaat
    14641 aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa tggattgttg
    14701 gaaaagatta cttgatccaa ccatttttta tccataaaat cctcttttgc aaaagtttct
    14761 aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat tggcttgtcg
    14821 ggcgtcagac cgattgctat ccaagaaaga agttgtgaga attcctccaa tgtacctaga
    14881 cctccaggga aaacgaggac agcatctccc ttttcaaata ataatacctt tctttcatcg
    14941 atcgtttctg taggaataaa tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc
    15001 ccattcggat agatacctgt gacttttccg ttattttcca gtactgtttt actaatcact
    15061 cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg tgccatgtat
    15121 tttcctaacg tttgcgcaat aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa
    15181 aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt cattctacac
    15241 gtgttttttt aaaataaaag actagatttt gtagtttttt agtggt
//
LOCUS       DAZAJZ010000070        14960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000070 DAZAJZ010000000
VERSION     DAZAJZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.070"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG86D_002085"
     CDS             4..2376
                     /locus_tag="ACG86D_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN3141214.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG86D_002086"
     CDS             2437..3897
                     /locus_tag="ACG86D_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN3141215.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG86D_002087"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG86D_002087"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3141216.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG86D_002088"
     CDS             6128..6277
                     /locus_tag="ACG86D_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141217.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG86D_002089"
     CDS             6352..6945
                     /locus_tag="ACG86D_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN3141218.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG86D_002090"
     CDS             6958..7857
                     /locus_tag="ACG86D_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN3141219.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG86D_002091"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG86D_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN3141220.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG86D_002092"
     CDS             8723..8983
                     /locus_tag="ACG86D_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141221.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG86D_002093"
     CDS             9428..9634
                     /locus_tag="ACG86D_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN3141222.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG86D_002094"
     CDS             9655..9885
                     /locus_tag="ACG86D_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141223.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG86D_002095"
     CDS             9901..10338
                     /locus_tag="ACG86D_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141224.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG86D_002096"
     CDS             10331..11038
                     /locus_tag="ACG86D_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141225.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG86D_002097"
     CDS             11202..11402
                     /locus_tag="ACG86D_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141226.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG86D_002098"
     CDS             11418..11597
                     /locus_tag="ACG86D_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141227.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG86D_002099"
     CDS             11922..12185
                     /locus_tag="ACG86D_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFN3141228.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG86D_002100"
     CDS             12179..12529
                     /locus_tag="ACG86D_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3141229.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG86D_002101"
     CDS             complement(12553..13167)
                     /locus_tag="ACG86D_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3141230.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACG86D_002102"
     CDS             13481..13903
                     /locus_tag="ACG86D_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141231.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACG86D_002103"
     CDS             13913..14116
                     /locus_tag="ACG86D_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141232.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACG86D_002104"
     CDS             14327..14911
                     /locus_tag="ACG86D_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3141233.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5512 a         2429 c         3034 g         3985 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga
//
LOCUS       DAZAJZ010000071        14437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000071 DAZAJZ010000000
VERSION     DAZAJZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.071"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACG86D_002105"
     CDS             complement(227..1114)
                     /locus_tag="ACG86D_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3141234.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACG86D_002106"
     CDS             complement(1111..1980)
                     /locus_tag="ACG86D_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3141235.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACG86D_002107"
     CDS             complement(2117..3403)
                     /locus_tag="ACG86D_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3141236.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACG86D_002108"
     CDS             3676..4254
                     /locus_tag="ACG86D_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3141237.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACG86D_002109"
     CDS             4254..5183
                     /locus_tag="ACG86D_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141238.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACG86D_002110"
     CDS             5180..6916
                     /locus_tag="ACG86D_002110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3141239.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACG86D_002111"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACG86D_002111"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN3141240.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACG86D_002112"
     CDS             complement(7812..8321)
                     /locus_tag="ACG86D_002112"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN3141241.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACG86D_002113"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACG86D_002113"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN3141242.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACG86D_002114"
     CDS             complement(9622..10713)
                     /locus_tag="ACG86D_002114"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN3141243.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACG86D_002115"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACG86D_002115"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN3141244.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACG86D_002116"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACG86D_002116"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN3141245.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACG86D_002117"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACG86D_002117"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN3141246.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4112 a         3144 c         2609 g         4572 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactga
//
LOCUS       DAZAJZ010000072        13971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000072 DAZAJZ010000000
VERSION     DAZAJZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.072"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACG86D_002118"
     CDS             complement(146..1111)
                     /locus_tag="ACG86D_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN3141247.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACG86D_002119"
     CDS             1345..2190
                     /locus_tag="ACG86D_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3141248.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACG86D_002120"
     CDS             2381..3568
                     /locus_tag="ACG86D_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN3141249.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACG86D_002121"
     CDS             3574..3930
                     /locus_tag="ACG86D_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN3141250.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACG86D_002122"
     CDS             3932..4270
                     /locus_tag="ACG86D_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN3141251.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACG86D_002123"
     CDS             4665..5699
                     /locus_tag="ACG86D_002123"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN3141252.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACG86D_002124"
     CDS             5692..6378
                     /locus_tag="ACG86D_002124"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN3141253.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACG86D_002125"
     CDS             6402..7250
                     /locus_tag="ACG86D_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3141254.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACG86D_002126"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACG86D_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN3141255.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACG86D_002127"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACG86D_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN3141256.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACG86D_002128"
     CDS             9516..10751
                     /locus_tag="ACG86D_002128"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN3141257.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACG86D_002129"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACG86D_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN3141258.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACG86D_002130"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACG86D_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN3141259.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12702..13844)
                     /locus_tag="ACG86D_002131"
     CDS             complement(12702..13844)
                     /locus_tag="ACG86D_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141260.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4545 a         2580 c         3014 g         3832 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tgggccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaacc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg
    12601 ttaataacta tttcattgct cgaaacgttg atattaaagc gtttcgagcg tttttttatt
    12661 tgtattttaa tggttggcta cgtttttggc tacgtttgga attattcgcc cataaatttt
    12721 gcaaatcggt caccagtttt cttaacagat tgtggtgtga catgagcata gatattcatg
    12781 gtagttttaa tatctttatg acctaatcgt tcctgtactt cttttatcga cgcaccactt
    12841 tcaaacaata agctacagtg ggtatgccta aatccatgtg gagtaatttg aggctccatc
    12901 ggtgtctttt tgtaaatcca gtctaaccaa tcgtttacta cttgggggta gtacaacttg
    12961 tttgtgtttg tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc atataagatc
    13021 attattttca tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa atcatcaagc
    13081 tgaatcgttc ttgatgaatt tttagtttta ggtacttgta gaactacttg attaaactcg
    13141 tcttgagcga tagttttacc aattgttatt gttttattga aatagtctat atcttcccat
    13201 tgaagcgcca atgcttcgct tttacgcatt cctgtataag ctaataaacg gaaaaaagtg
    13261 tacagtttaa ctgaattatt ttctttagta ttctgtaaaa acagttgtaa ttgatctttc
    13321 gtgtaaaact tcaagggttg ttcttcttca atttttcttg gaagcaatgt tttagacata
    13381 ggattatcct tacagacttc cattgctact ccaaatttaa atatttgagc agttgttttt
    13441 ctcaagaaac catattgttt taatgttttg tgccattcat tcacctgttt ttggcaatag
    13501 ctaacactta ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg gtttttgcaa
    13561 aaacgttgag caacggcaac agaagacggt tttactcttg ttctgtagtt atcaatccat
    13621 aattcataca actcttgaaa agttgtaaca ttgattttaa actcttgttt ttctttaaag
    13681 tctacaagta gtctattaag tgctgtttgc gcctcagctt ttgatttgaa tcttctacgc
    13741 tttgtacgta tatcttttcc agtcttggga tctttcccta aataagtttc aaatccccaa
    13801 tatttttcgc cattttgtaa tttataacta aaaaatgttg ccatttgatt ctctccttac
    13861 ttcattattg caggtagaga agttatttga agaatcaatt atttttaaat ttatcgtgat
    13921 tatcagttaa aaattttgcg attatactat ttttctcata tgggaaattg t
//
LOCUS       DAZAJZ010000073        13957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000073 DAZAJZ010000000
VERSION     DAZAJZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.073"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACG86D_002132"
     CDS             112..306
                     /locus_tag="ACG86D_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141261.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACG86D_002133"
     CDS             575..874
                     /locus_tag="ACG86D_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141262.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACG86D_002134"
     CDS             complement(971..1405)
                     /locus_tag="ACG86D_002134"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3141263.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACG86D_002135"
     CDS             complement(1637..2347)
                     /locus_tag="ACG86D_002135"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN3141264.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACG86D_002136"
     CDS             complement(2337..3197)
                     /locus_tag="ACG86D_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN3141265.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACG86D_002137"
     CDS             complement(3202..3840)
                     /locus_tag="ACG86D_002137"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN3141266.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACG86D_002138"
     CDS             complement(3855..4154)
                     /locus_tag="ACG86D_002138"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3141267.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACG86D_002139"
     CDS             complement(4197..5681)
                     /locus_tag="ACG86D_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN3141268.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACG86D_002140"
     CDS             complement(5701..6162)
                     /locus_tag="ACG86D_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3141269.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACG86D_002141"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACG86D_002141"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN3141270.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACG86D_002142"
     CDS             7465..8235
                     /locus_tag="ACG86D_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3141271.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACG86D_002143"
     CDS             complement(8508..9335)
                     /locus_tag="ACG86D_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141272.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="ACG86D_002144"
     CDS             9903..11138
                     /locus_tag="ACG86D_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3141273.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="ACG86D_002145"
     CDS             complement(12137..12397)
                     /locus_tag="ACG86D_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN3141274.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="ACG86D_002146"
     CDS             complement(12424..13725)
                     /locus_tag="ACG86D_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3141275.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4510 a         2845 c         2185 g         4417 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata
    13801 taattaagaa attttatttc aatatattct cttaaataat caaatttatt tttaagaatc
    13861 ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc
    13921 aaaatgatta aatctagtga ttgaaacata tcatttc
//
LOCUS       DAZAJZ010000074        13819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000074 DAZAJZ010000000
VERSION     DAZAJZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.074"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..2259
                     /gene="acm"
                     /locus_tag="ACG86D_002147"
     CDS             94..2259
                     /gene="acm"
                     /locus_tag="ACG86D_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN3141276.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2531..3016)
                     /locus_tag="ACG86D_002148"
     CDS             complement(2531..3016)
                     /locus_tag="ACG86D_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN3141277.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3106..3451
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3496..4137
                     /locus_tag="ACG86D_002149"
     CDS             3496..4137
                     /locus_tag="ACG86D_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3141278.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4156..4791
                     /locus_tag="ACG86D_002150"
     CDS             4156..4791
                     /locus_tag="ACG86D_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3141279.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4809..5624
                     /locus_tag="ACG86D_002151"
     CDS             4809..5624
                     /locus_tag="ACG86D_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3141280.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5943..7379
                     /locus_tag="ACG86D_002152"
     CDS             5943..7379
                     /locus_tag="ACG86D_002152"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3141281.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7568..10000
                     /locus_tag="ACG86D_002153"
     CDS             7568..10000
                     /locus_tag="ACG86D_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN3141282.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10230..11297
                     /gene="rlmN"
                     /locus_tag="ACG86D_002154"
     CDS             10230..11297
                     /gene="rlmN"
                     /locus_tag="ACG86D_002154"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN3141283.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRSEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11287..11493
                     /locus_tag="ACG86D_002155"
     CDS             11287..11493
                     /locus_tag="ACG86D_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141284.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11490..12593)
                     /locus_tag="ACG86D_002156"
     CDS             complement(11490..12593)
                     /locus_tag="ACG86D_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3141285.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13145..13693
                     /gene="aac(6')"
                     /locus_tag="ACG86D_002157"
     CDS             13145..13693
                     /gene="aac(6')"
                     /locus_tag="ACG86D_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN3141286.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4730 a         2295 c         2932 g         3862 t
ORIGIN      
        1 attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt
       61 ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta
      121 tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat
      181 gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca
      241 gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca
      301 ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa
      361 aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat
      421 tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg
      481 caatttgaga tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc
      541 accgtgacga gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct
      601 tcttctagcg ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt
      661 cgatggtttt tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt
      721 gatgaaatcc aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac
      781 ttaggtgaac agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc
      841 cccagcgcta catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa
      901 aactttgtca atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa
      961 caaataaatt ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt
     1021 gatggagaaa gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt
     1081 acagttagag gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca
     1141 aatgtgactt tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc
     1201 ctgttgactt cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat
     1261 gttgtaaaag aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa
     1321 ctcaaattct ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag
     1381 aaaaaggtta cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg
     1441 agtatttatt ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca
     1501 gaaacaaaac gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat
     1561 gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat
     1621 tatgtccctt caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa
     1681 gaagtcattt cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa
     1741 cgaccgacga cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa
     1801 gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg
     1861 caagaaatca tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac
     1921 gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc
     1981 catataacac cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta
     2041 ttctctcaaa aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat
     2101 tatattagta agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct
     2161 aaaacaagta gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc
     2221 tgtgctggga tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt
     2281 taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt
     2341 ttatatatgc ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa
     2401 tactcgtatt agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa
     2461 tttctgatat attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac
     2521 tactctatca ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt
     2581 caaagtcgga aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg
     2641 tatcttgacg atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca
     2701 tcaatgctaa tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt
     2761 cagaaacaaa agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt
     2821 gcttttcatc aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata
     2881 gattttttac ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag
     2941 gatgatcgac tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt
     3001 cggttgcacg actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag
     3061 ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa
     3121 agaagagtag ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt
     3181 aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag
     3241 cccgttacag ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt
     3301 cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt
     3361 ttgttttcat ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca
     3421 gaggtggaac cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta
     3481 aaggaggaaa agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca
     3541 ctcacaacat tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt
     3601 ttagcattag gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta
     3661 tgggttatgc ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg
     3721 acgattggga tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat
     3781 gcagcttatt ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat
     3841 gaagcagcgc aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca
     3901 caagtgacaa agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat
     3961 tcttccttga tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg
     4021 gaacgcgacg cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact
     4081 ggtattctaa caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg
     4141 ataggaggaa gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa
     4201 ataatcaaag atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct
     4261 tccggtggcg gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc
     4321 ggagaatttt atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa
     4381 gttgtaggag tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac
     4441 attaccttag caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca
     4501 gaagaattag cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt
     4561 tctggcggac aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta
     4621 ttggcttatg atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt
     4681 ttgatcctat cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta
     4741 tttgcagaaa atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga
     4801 agatagagat gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg
     4861 caggctgttc gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa
     4921 aaatcgttat aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga
     4981 agctcacagg atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg
     5041 tagactttca gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg
     5101 atttgatatg gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa
     5161 aaccttatat ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact
     5221 ttggacaaat gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat
     5281 ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg
     5341 atagttttaa tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg
     5401 ataaagtata cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa
     5461 caaaaggttc ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat
     5521 tagtgaaaaa aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa
     5581 tatccgataa atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg
     5641 acaaaagacg tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg
     5701 tgactcggag ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt
     5761 tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
     5821 tactactcga aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg
     5881 gaaggggttt cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat
     5941 gaatggaaca caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg
     6001 cagcgtatca agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc
     6061 gatttgtacg gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc
     6121 actatcatcg ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc
     6181 gcttctctgt agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg
     6241 ggctcgcttt ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt
     6301 tgactttata tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat
     6361 cacgtcagat cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg
     6421 ggaaagtcca ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt
     6481 tgttagcagc acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata
     6541 ttgctaattt agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa
     6601 aaatcggacc aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg
     6661 ttctggctgc tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt
     6721 ggggaaaata tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga
     6781 ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt
     6841 actatcaaac ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga
     6901 atacgacagg aaaaaaaagt tcatcagaag aaactggagt tcctggaatg tataaaaaag
     6961 ttgagaatcc atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa
     7021 ggatcggact gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg
     7081 gcttaggaga gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca
     7141 attacttaca aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc
     7201 taggctattg cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc
     7261 gatatggatt tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata
     7321 agaaagacag ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt
     7381 tcacatactt aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc
     7441 atttagaaat taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt
     7501 tcaaacaaaa ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg
     7561 ataataaatg gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa
     7621 aaagcaaaaa cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt
     7681 atttgctgtc ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt
     7741 tcggatcatt gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt
     7801 ttggattgtc attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg
     7861 catactcttt tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca
     7921 tactggggaa ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca
     7981 aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac
     8041 ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg
     8101 agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg
     8161 agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc
     8221 aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa
     8281 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     8341 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     8401 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     8461 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     8521 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     8581 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     8641 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     8701 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     8761 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     8821 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     8881 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     8941 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     9001 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     9061 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     9121 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     9181 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     9241 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     9301 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     9361 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     9421 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     9481 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     9541 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     9601 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     9661 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     9721 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     9781 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     9841 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     9901 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     9961 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
    10021 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
    10081 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
    10141 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
    10201 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
    10261 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
    10321 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
    10381 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
    10441 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
    10501 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
    10561 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
    10621 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
    10681 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
    10741 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
    10801 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
    10861 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
    10921 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
    10981 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
    11041 gatcagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
    11101 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
    11161 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
    11221 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
    11281 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
    11341 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
    11401 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
    11461 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
    11521 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
    11581 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
    11641 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
    11701 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
    11761 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
    11821 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
    11881 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
    11941 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
    12001 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
    12061 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
    12121 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
    12181 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
    12241 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    12301 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    12361 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    12421 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    12481 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    12541 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    12601 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    12661 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    12721 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    12781 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
    12841 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
    12901 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
    12961 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taatttttta
    13021 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
    13081 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
    13141 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
    13201 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
    13261 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
    13321 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
    13381 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
    13441 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
    13501 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
    13561 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
    13621 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
    13681 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
    13741 aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta
    13801 cttttggaac accgtgtac
//
LOCUS       DAZAJZ010000075        13805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000075 DAZAJZ010000000
VERSION     DAZAJZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.075"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..2130)
                     /gene="glyS"
                     /locus_tag="ACG86D_002158"
     CDS             complement(55..2130)
                     /gene="glyS"
                     /locus_tag="ACG86D_002158"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN3141287.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2132..3049)
                     /gene="glyQ"
                     /locus_tag="ACG86D_002159"
     CDS             complement(2132..3049)
                     /gene="glyQ"
                     /locus_tag="ACG86D_002159"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN3141288.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3433..4245)
                     /gene="recO"
                     /locus_tag="ACG86D_002160"
     CDS             complement(3433..4245)
                     /gene="recO"
                     /locus_tag="ACG86D_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN3141289.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4388..5287)
                     /gene="era"
                     /locus_tag="ACG86D_002161"
     CDS             complement(4388..5287)
                     /gene="era"
                     /locus_tag="ACG86D_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN3141290.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5302..5697)
                     /locus_tag="ACG86D_002162"
     CDS             complement(5302..5697)
                     /locus_tag="ACG86D_002162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3141291.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5681..6157)
                     /gene="ybeY"
                     /locus_tag="ACG86D_002163"
     CDS             complement(5681..6157)
                     /gene="ybeY"
                     /locus_tag="ACG86D_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN3141292.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6174..8366)
                     /locus_tag="ACG86D_002164"
     CDS             complement(6174..8366)
                     /locus_tag="ACG86D_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN3141293.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8390..9361)
                     /locus_tag="ACG86D_002165"
     CDS             complement(8390..9361)
                     /locus_tag="ACG86D_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN3141294.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10067..11446
                     /gene="brnQ"
                     /locus_tag="ACG86D_002166"
     CDS             10067..11446
                     /gene="brnQ"
                     /locus_tag="ACG86D_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN3141295.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11600..12046)
                     /locus_tag="ACG86D_002167"
     CDS             complement(11600..12046)
                     /locus_tag="ACG86D_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN3141296.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12073..12249)
                     /gene="rpsU"
                     /locus_tag="ACG86D_002168"
     CDS             complement(12073..12249)
                     /gene="rpsU"
                     /locus_tag="ACG86D_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN3141297.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12411..12839)
                     /locus_tag="ACG86D_002169"
     CDS             complement(12411..12839)
                     /locus_tag="ACG86D_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3141298.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12931..13791
                     /locus_tag="ACG86D_002170"
     CDS             12931..13791
                     /locus_tag="ACG86D_002170"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN3141299.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3773 a         2875 c         2495 g         4662 t
ORIGIN      
        1 ccagagaaat agcctatttt gtgcctgttc tctggtttat ttacatatta gaaattattt
       61 tgtgatgatt tgatctaaac ttgctagtga caaagccatg gcagcaattt gacttaattg
      121 cgttaatcgg ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata
      181 attttcgatc agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc
      241 agcaaagctt gttttcattg cctctactgc tttgtgcagt tctttctccg cttcgttttc
      301 gaacagttca gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac
      361 gacacgtgtc aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa
      421 caattgtgct gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc
      481 gatgacatca tgacggatat tcttcgttgt caataattgg cgcagacggc ctttgataaa
      541 gtcgatcacc tgttgttgtc ccgctgacaa gccaatgcca aaacgatccg tatctttact
      601 gataacttca ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc
      661 gatgattcga acgacaccat atgcttgacg gcgcaaagca tatggatcgt ttgagccact
      721 tgggatcatc ccaactgcaa agaaagaaaa gatactatct aatttatcag caatcgctaa
      781 aacagaacct actgtagtct caggtaaatc tccttcactg gatataggca tgtaatgttc
      841 acggatcgct gttgctacgg ctggtttctc gcctttaagc agagcatatt tttcgcccat
      901 gatcccttgt agttcaggga attctcctac catgttggtt actagatcaa atttatagat
      961 ttcagcagcg cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgcctat
     1021 gatgcgagcg atgacttgta cccgttgcat tttctcatag atgctaccga ttttttcatg
     1081 gaaagtaaca gtttttaatt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt
     1141 ataaaagaat tccgcatctt ctaaacgagc agtcaatact ttttgattgc ctcgtaccac
     1201 attttccaat tttacccgat cgccattacg agcagaaata aaatgaggaa gcaacatacc
     1261 ttgatcgttc cgtacttcga aataacgttg atgttctttc atagaagtga ctagtacttc
     1321 ttcaggaaca gatagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac
     1381 taaattgttg acttcttcta gtaaatcttc atctagatca atgatccagt gatttttttc
     1441 tgctaattcg tttgcttgtg tgatgatcat ttcttttctt ttttgcggtg aagcaataac
     1501 aaattgttca ttcaattttt cttcgtattg ttttgcatgc gtaaatgtca catcttcgcc
     1561 taagaaacga tgacctcttg aagctctgtc tgttttgaca tccaagatct caaacgggat
     1621 tatttcgtca tctaacaatg ccacgatcca gtgaataggg cgaatatatt caaaatcgta
     1681 ttctgcccag tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt
     1741 caatacttct tttgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc
     1801 ttttaattct ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc
     1861 agctgctttc gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac
     1921 ttcttcttcg ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg
     1981 cggtgtagaa aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc
     2041 tgttttttct tctaattgtt tcatgcttgg tgtgactaca tgagcaggta tttcttccag
     2101 cccgatttca agtaataagt cttttgccat cttattcagc ctccttcacg ttttctggat
     2161 ttgctgtgtt tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt
     2221 tggctacagc acgagccatg ttgcggatac gggaaagata acctgcacgc tctgttacgg
     2281 atactgcacc gcgagcatct aataaattga atgtatggct acatttcaaa atataatcat
     2341 acgctggatg aacaagattt tcatcgatac agcgtttagc ttctttttca aaacgatcaa
     2401 aattgcttag tagcaggtct tggtcgctta cttcaaaaga atatttagaa tgttcatatt
     2461 ctggttgttt gaagatctca ccatatttca cgccatcagc ccattccaga tcataaacac
     2521 tttcaacttc ttggatataa gaagcaagac gttctagtcc ataagtgatc tcagaagtaa
     2581 ctggtttaca aggtagtcct ccgacttgct ggaagtatgt gaattgtgtg atctccatcc
     2641 catcaagcca tacttcccag cccacaccgg cacaacccat cgatgggttc tcccagttgt
     2701 cttcaacaaa gcgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta
     2761 aataaagctc ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct
     2821 gatacaaacg gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta
     2881 cataagcagc attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg
     2941 tacctgctcc tttttctgta tcataagctt gcatcagcat gcagccgttt gatgaccaaa
     3001 atttttgtaa tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc
     3061 ctttcaattt ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc
     3121 ctatgtctga ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata
     3181 aagattcggc agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc
     3241 ggcttccact ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac
     3301 gtattcattt tgcagttttc tggtttgtat aatagatcct ctcagtgaac gatctattta
     3361 atctacggtt aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt
     3421 tgtatctttc atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg
     3481 cgtctttaat acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg
     3541 aataccgaca tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc
     3601 ttctttcaag tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa
     3661 ataaacagcg cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca
     3721 tatcacaccg ccatatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac
     3781 acagtgcgtc caaattggcg cgattccgaa gcgttgcagt atttgcactt caaagatatt
     3841 cgtgatgatt tctgcatcat ctccttggtc catccgctgc agagcttgtt gcgtaaattg
     3901 gaataaatgc gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata
     3961 cgtaccataa ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt
     4021 gacactgctt aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt
     4081 aaaggggagc aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa
     4141 catcattttg ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt
     4201 acggctagat aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa
     4261 aaataaatct ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgct
     4321 gtttttctca acgtacggat ttttatccag tttatttcta tacctaacct ataagaagtc
     4381 cagaatatca gtactcgtct tttcggtagc cgaagtcttg cagatagact tttttgtctc
     4441 gccagtcttt ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt
     4501 cgatatcctt tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa
     4561 tgattccttt ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat
     4621 tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg
     4681 ttaaaaacaa gactttctca cggatcaatt cagaaacgat gaagtattca ggatgatcag
     4741 tcacttgatc atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca
     4801 ttaagcgttc gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca
     4861 tctgcgtcga ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt
     4921 tattgataat cagataaaca ggagaatgca cgttttttag acgttcaata ataaaatcgt
     4981 ctccccggcc acgtttttga tcagcactga tcataaataa gactgcatcc acttctttca
     5041 acgcactgta agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc
     5101 ctggtgtatc gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat
     5161 ttctagttgt ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca
     5221 ataatgtgga ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt
     5281 ctggcatata cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa
     5341 aatcaaccca atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc
     5401 tttgattttt ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc
     5461 aaaaacagta ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa
     5521 ccactctatc cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc
     5581 aacatgtttc ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa
     5641 ttccacagac atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg
     5701 catccaagat ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt
     5761 catacccgtt gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg
     5821 aatggccgta agcttctgct tgttcagcag cacgttcaat agagatcatg atgtctccta
     5881 gtacacgagg taatggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt
     5941 cttcttccag agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat
     6001 tgattacttg gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca
     6061 gctttaaata agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg
     6121 agacttggtt tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct
     6181 tcttgttctt cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa
     6241 tggaaggtgc tacttaaacc gctgattaat gatttttcca cttttcggat ttctttcaag
     6301 gtcagtccac tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca
     6361 aaactttcga ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg
     6421 tcagcaatat tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat
     6481 tgctcttctg ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc
     6541 gtggttccat gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc
     6601 aatattttgg ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
     6661 aagaaattgt gcgggttttc cgctcctgaa ggcaagtttt caacgaaaaa gctagcatgt
     6721 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg
     6781 atttctgcta cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct
     6841 ggcgcttctt ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat
     6901 acaattacac tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc
     6961 aagaatgaga agagtgcacc agtgaacccg cagacgagca ttaaccaagt cgttgcatca
     7021 ccaaagctca ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg
     7081 acccacatgg cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc
     7141 atgagcccag agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa
     7201 tcataaaaaa tgaaaagtgc ggatactgct tggaaaatac cagccatgat ccctgctctt
     7261 cgattcaaga aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata
     7321 taagtagctt gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac
     7381 atagctgtca catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt
     7441 gaattataaa tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aattagcgga
     7501 aaaatcgaag ttgtttgatt agtcagtcct agtaattcca attttttcat agctgaagca
     7561 tcgatttgat taccttcacg aacaatgatt tctccttgga agatcatcac tggttgtaca
     7621 gcttctcgtg cggattgttt caattcttct gtctttttct cattcaatac gtcattcact
     7681 acgatacctt gatccactaa gtagcgaatc atttgttgtt gtgctggtgt gacattcaaa
     7741 tattggattt gctcttctgc ttcttgttta aaagcttcca attcactttc acgaacttgt
     7801 ttgctcatct gttcgtcaat gagcttcaga ctctcatccc tcacagtatg aatctcagtt
     7861 tcagtcattt caaatatcgt cgaataaaaa ttgttcggta gtttttgata aaacacaaca
     7921 ttttctgcat tcacattttc aaaatttttc ttcagcgcgg cgatccgctc ttctgtagta
     7981 ggctttggga tattttcatc ttcctttgcc ttatcaatct tttcgtcata agatttgttg
     8041 atggcatcat tcgtcttatc gatcaattca aatagttgct tgatccgatt gttctggtca
     8101 tctgcaagat ctttttggta agtgtattct ggagtgactg cttctgctgc cagttttctt
     8161 ttttgttcgg tttcttccgt gttctcgatc gttttgttcg cacggatact ttcttctgct
     8221 acttggcctt ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag
     8281 ataatgataa aaaacaagaa cagcaggaaa ggcaatgctg cctttccaag tttttcttgt
     8341 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
     8401 ggagatctgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
     8461 cacttgcttc aaaattaaca aaatcgattt tcttgatttg ctttaatgtt cgttctgcat
     8521 gaacaagacc acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa
     8581 tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt
     8641 tttgtgcttc gtccaagata acgaatgcat catccaatgt tcgcccccgc atgtaagcta
     8701 aaggtgcgat ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa
     8761 tttggtataa agcatcataa acaggccgca gataaggatc cactttttct tttagatccc
     8821 cgggaagaaa gcctagattt tcacctgctt ccacagcagg ccgcgtcaaa atgatttttt
     8881 gcacttcacc ttttttcaag gcagcgattg ctaatactac tgcaagaaaa gtttttcctg
     8941 taccggctgg tcctacgcca aatacgatat catgtttggc taccgattcg acatatttct
     9001 tttggccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
     9061 catacatctc aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacaa
     9121 catcgggcgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg
     9181 aggcagccaa ggaaatctgg gattgctcac caatgatctg aatgatttct ccacgtgtat
     9241 ggatggttgt ttccgttgtt tcctcaatca gttttatatg cttgtcgtgt gcacctaaaa
     9301 gcatgctgac atcatcagca gccgtcaatc taatctctaa agaactagac tcttccgtca
     9361 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
     9421 ctatatagca aataagaaaa ggttctaaaa gagttgctta accataagta ccaagaaacg
     9481 ttaaaaaact agccgcttcc attctctaaa ggtctcgttt gaataaaaaa agctgatcat
     9541 gtaagctatg cataagatag aggacaagta ttccgtctaa cttagcaagt attctctggg
     9601 tatttttctt gtttcaaaaa aagaaaacaa aatatttgaa atattcttgc atttcaaaaa
     9661 aaacaggtgt attataacgt caacaaataa atatacagca aagcgtcgag gaaaacagta
     9721 ggctattcag tgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat
     9781 agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta
     9841 tcaggctagc ggataacttt atcgaaaagc gtccgtgaat gaagaagctt ttttaagcga
     9901 aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt
     9961 tatattttaa ggtggtggtt ttcatggaat ctgatattag tggtatttct agtttctgca
    10021 acgtttattt tgttactcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa
    10081 aacttattca tggaagcaat tattgatggt cagttcactt atcttcggga tgttcttcgg
    10141 gtcaggtaat ctgatctttc ctgtacactt aggtcaaatg gctggatcaa actggatcag
    10201 cgctggtgta ggatttgcca tctcaggcgc tcttttccca ttattagcta tcttagctgt
    10261 agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc
    10321 tgccttgttc ttgatccttg ttcatttaac gatcggacca ttctttggta caccgcggac
    10381 agcagcaact gcttttgaaa tggctgctaa accctttttg cctgaaaaat atgattcatt
    10441 aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta ctgtacatcc
    10501 aacacgtttg gtcaaatatg tcggaaagta tttaaactcc ttgtttcttg cattattagc
    10561 tatcattttt gtcgtttctt tattccaacc catggggaat ctaaaccaat ctgctgcaga
    10621 ttcttatcag acgaacgctt tgacaaatgg cgttttagaa ggatacaaca cggttgatgc
    10681 agtagcttta cttgctttaa gtgtcacttt tgtccatgca gttcgtgatc ttggttttaa
    10741 agataaaaaa gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgttagaagt
    10801 catgatttat tttggtctgg tccttctcgg tgcgatgagt ttgaataaga tgtctttatc
    10861 agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta acttcggtgc
    10921 tagcttctta ggggtaatgg tcacattagg tgtcttcact actgctatgg gactcgttgt
    10981 ttcttttgca caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg
    11041 gttaacgact tttgtctctt ttgtcgttgc gaatgccgga ttggataata tcatccaatg
    11101 gtctttacca gtattgatgc ttttgtatcc attgtcactt gcgttaatct tactttcctt
    11161 aacagcaaaa ttcttccaaa agagtccatt tgtctatcaa gtaacgatgt tcttttctgc
    11221 cgttccagcc gtattggata tgttggcaaa ttcgcctgca ttagtgagtc agcaaactgt
    11281 cgtagcaagc ttactaggat tttatcatca ttatgttcct tttgctgcat taggtcttgg
    11341 ctgggtcgtc ccaacgttgc ttggttacgc tggcagcttg ctcttttatt atgtatatcg
    11401 cgtatcagga tataaacaag aagcaagtga gttaccagaa gaataaccaa atcaaacaca
    11461 atttaacata agagatatta aacaggaaaa aaacagctag aagaaagaat aagattcttt
    11521 cttctagctg ttttttagtt ctttttttac gaacacaaaa aactgcggat aatcactgtc
    11581 cgcagtttaa atgataaaat taagattgta atagttcttt cacaatcgca ttcacttggt
    11641 ttccatctgc ttttcctttg actttaggca taattgcacc cataactttg ccaaattctt
    11701 tcattgatga agcaccagtt tgagtgattg cttcttgaac aagttgtcga atttcttcgt
    11761 cggaaagttg ttccggcata tatttttcga ctattacaat ttcacttttt actttttcag
    11821 ctaaatcgtc acgtcctgct tcttcaaact catgcaaaga atcacgtcgc tgtttcattt
    11881 cacgagacaa caccgttagt tcttcttctt ctgtcaagtc gtgaccttct ttaatctgct
    11941 cgttttgtat agatgacttg atcattcgga tgacttgtaa agattcctta tctttcgctt
    12001 tcatcgctgc tttcatgtca tcgtttaatc tacttagtag tgacacacta ccaactccaa
    12061 ttcatcacag aactagaatt ttttgcgttt tctagctgct tcagattttt tcttgcgtct
    12121 tacacttggt ttttcgtaga attcacgttt acgagattct tgtaaagtac ccgcttttga
    12181 cacggaacgt ttgaagcgac gaagagcatc gtcaagtgat tcgtttttgc gaacgactgt
    12241 ttttgacatg ttaattccct ccctccgagc ttaaaaagta accgtaattg ttattgaaat
    12301 atatctcaaa aaacaagact gtcctgtaaa tattatatct aatgctgttt tgaccgtcaa
    12361 ggaaatcttg ctttttttta aaattatcaa atgttatttt atttaaatcc ttattgacaa
    12421 ttttcacatc ttccgaaaat ctcgaatcta tgcccttcga tcgtgcatcc acctagctgt
    12481 tcttcgaaat aattcatcgg acacatatgg atttctttcg ttgttccgca cactgtacag
    12541 ataaaatgat ggtgatggcc gattttcccc tgacagcaat gaaaacgaaa cttcatttct
    12601 ccatttaaat cggtttcttc cagtaaatcg atctctgcaa aatcatggag attccggtaa
    12661 atcgtatcat aacttacccc tttgaattcc tgattcatat agtcaaaaac ttctttagca
    12721 gatacgtagc ggttacgctt cactaaataa gttaacaatg cttctctttt tttagtatat
    12781 ttaaaaccgt tatttttcaa tgtttctaat gattgttcga tcaatgtctg ttttttcatg
    12841 acttattcct ccaaatcaaa atgattacga agtacgcatt ctagagtatg gtataataaa
    12901 cataaaaaaa atgcaagtga ggttttatcc atgtcttccg attctacaga aatcgttact
    12961 ttttttgtca tctccgactc tgctggagaa actgcgacaa aactagcgca agctactatg
    13021 gctcagtatc ctaccgttga gttcaaccta ttccgcagaa cgttcgtgac agatgaaaaa
    13081 acattaaggc aggcattgca agatgcatta gaagaaaaag cacttgtcct gcatacatta
    13141 atcaatcaag aactgatcga gataacacgt tctttttgtg aagaacatca attatttagt
    13201 tttgatgtga tgacgccacc gatatcagaa attgaacgtt taactggtat caagccaatt
    13261 cggcaacctg gtgccctcca tttattgaat gagaactatt tcaaacggat caaagcaatg
    13321 gaatttgctg taaaatacga tgatgggaaa gatcctcgtg gttttttaga agcagatgtc
    13381 gttttgctag gcgtatcccg aacttccaaa acgcctttga gcttgttttt agctaacaaa
    13441 aatttaaaag ttgccaattt acctttgatc ccacaagcac atattcccaa acagctttgg
    13501 gagatcgatc ctaaaaaaat cgtgggacta acgaataacc cagatatttt aaataatatc
    13561 cgtaaagaac ggatgcgctc ttacggatta aaccctgata ctgcttattc cgatatcgaa
    13621 aaaattagag ctgaacttga ttttgcgaat gatctttatg aaaaactagg atgtgtagtg
    13681 atcaatgtgg catctttatc tattgaagaa acggcttcgc ttattttgaa tgctttagac
    13741 ctagaagatc atagttatta tggaactgaa acaacagaaa aaagcgatta atcatttttg
    13801 tcttt
//
LOCUS       DAZAJZ010000076        13373 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000076 DAZAJZ010000000
VERSION     DAZAJZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.076"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..575)
                     /locus_tag="ACG86D_002171"
     CDS             complement(33..575)
                     /locus_tag="ACG86D_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN3141300.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            710..1099
                     /locus_tag="ACG86D_002172"
     CDS             710..1099
                     /locus_tag="ACG86D_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141301.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1103..1867
                     /locus_tag="ACG86D_002173"
     CDS             1103..1867
                     /locus_tag="ACG86D_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3141302.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            1996..2943
                     /locus_tag="ACG86D_002174"
     CDS             1996..2943
                     /locus_tag="ACG86D_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3141303.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3073..3813
                     /locus_tag="ACG86D_002175"
     CDS             3073..3813
                     /locus_tag="ACG86D_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141304.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4104..4772)
                     /locus_tag="ACG86D_002176"
     CDS             complement(4104..4772)
                     /locus_tag="ACG86D_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3141305.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4906..5193
                     /locus_tag="ACG86D_002177"
     CDS             4906..5193
                     /locus_tag="ACG86D_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141306.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5609..7147
                     /locus_tag="ACG86D_002178"
     CDS             5609..7147
                     /locus_tag="ACG86D_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN3141307.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7144..8109
                     /locus_tag="ACG86D_002179"
     CDS             7144..8109
                     /locus_tag="ACG86D_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141308.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8096..9418
                     /locus_tag="ACG86D_002180"
     CDS             8096..9418
                     /locus_tag="ACG86D_002180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3141309.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9481..9585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9659..10402
                     /locus_tag="ACG86D_002181"
     CDS             9659..10402
                     /locus_tag="ACG86D_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3141310.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10417..11409
                     /locus_tag="ACG86D_002182"
     CDS             10417..11409
                     /locus_tag="ACG86D_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3141311.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11421..12158
                     /locus_tag="ACG86D_002183"
     CDS             11421..12158
                     /locus_tag="ACG86D_002183"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141312.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12148..12870
                     /locus_tag="ACG86D_002184"
     CDS             12148..12870
                     /locus_tag="ACG86D_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN3141313.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGILTEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12954..>13373)
                     /locus_tag="ACG86D_002185"
     CDS             complement(12954..>13373)
                     /locus_tag="ACG86D_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN3141314.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4402 a         2289 c         2785 g         3897 t
ORIGIN      
        1 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
       61 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      121 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      181 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      241 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      301 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      361 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      421 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      481 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      541 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      601 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      661 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      721 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      781 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      841 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      901 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
      961 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1021 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1081 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1141 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1201 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1261 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1321 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1381 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1441 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1501 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1561 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1621 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1681 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1741 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1801 acaaatacaa ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1861 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1921 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     1981 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2041 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2101 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2161 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2221 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2281 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2341 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2401 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2461 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2521 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2581 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2641 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2701 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2761 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2821 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2881 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     2941 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3001 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3061 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3121 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3181 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3241 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3301 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3361 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3421 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3481 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3541 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3601 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3661 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3721 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3781 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3841 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa agtttatatc
     3901 gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta agaaaataat
     3961 ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta
     4021 taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt ctgtaaatta
     4081 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
     4141 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
     4201 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
     4261 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
     4321 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
     4381 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
     4441 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
     4501 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
     4561 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
     4621 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
     4681 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
     4741 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
     4801 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
     4861 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
     4921 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
     4981 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
     5041 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     5101 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     5161 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     5221 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     5281 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     5341 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     5401 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     5461 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     5521 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     5581 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     5641 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     5701 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     5761 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     5821 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     5881 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     5941 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     6001 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     6061 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     6121 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     6181 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     6241 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     6301 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     6361 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     6421 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     6481 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     6541 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     6601 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     6661 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     6721 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     6781 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     6841 caataatgga gatgttaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     6901 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     6961 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     7021 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     7081 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     7141 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     7201 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     7261 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     7321 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     7381 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     7441 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     7501 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     7561 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     7621 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     7681 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     7741 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     7801 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     7861 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     7921 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     7981 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     8041 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     8101 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     8161 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     8221 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     8281 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     8341 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     8401 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     8461 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     8521 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     8581 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     8641 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     8701 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     8761 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     8821 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     8881 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     8941 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     9001 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     9061 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     9121 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     9181 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     9241 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     9301 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     9361 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     9421 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     9481 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     9541 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     9601 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     9661 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     9721 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     9781 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     9841 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     9901 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
     9961 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
    10021 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
    10081 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
    10141 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
    10201 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
    10261 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
    10321 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
    10381 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
    10441 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
    10501 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
    10561 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
    10621 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
    10681 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
    10741 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
    10801 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
    10861 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
    10921 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
    10981 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
    11041 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
    11101 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
    11161 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
    11221 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
    11281 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
    11341 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
    11401 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
    11461 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
    11521 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
    11581 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
    11641 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
    11701 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
    11761 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
    11821 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
    11881 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
    11941 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
    12001 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
    12061 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
    12121 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
    12181 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
    12241 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
    12301 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
    12361 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
    12421 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
    12481 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
    12541 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
    12601 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
    12661 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
    12721 gctttagggg ggattttaac agaaaaagat tatgtctcac tgcttgaaga acagttaaag
    12781 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
    12841 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
    12901 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
    12961 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
    13021 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
    13081 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
    13141 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
    13201 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
    13261 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
    13321 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZAJZ010000077        13369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000077 DAZAJZ010000000
VERSION     DAZAJZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.077"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..2497
                     /gene="leuS"
                     /locus_tag="ACG86D_002186"
     CDS             83..2497
                     /gene="leuS"
                     /locus_tag="ACG86D_002186"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN3141315.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2845..4398
                     /locus_tag="ACG86D_002187"
     CDS             2845..4398
                     /locus_tag="ACG86D_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN3141316.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4779..6563)
                     /locus_tag="ACG86D_002188"
     CDS             complement(4779..6563)
                     /locus_tag="ACG86D_002188"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141317.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6578..8299)
                     /locus_tag="ACG86D_002189"
     CDS             complement(6578..8299)
                     /locus_tag="ACG86D_002189"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141318.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8399..9160
                     /locus_tag="ACG86D_002190"
     CDS             8399..9160
                     /locus_tag="ACG86D_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3141319.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            10127..10395
                     /locus_tag="ACG86D_002191"
                     /pseudo
     CDS             10127..10395
                     /locus_tag="ACG86D_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            11067..>13369
                     /locus_tag="ACG86D_002192"
     CDS             11067..>13369
                     /locus_tag="ACG86D_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3141320.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         4401 a         2289 c         2741 g         3938 t
ORIGIN      
        1 ataaaaataa acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt
       61 caatagaaca ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca
      121 aaaatactgg gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt
      181 ttacgcactg gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga
      241 aggttatacg gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct
      301 gcatccaatg gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg
      361 aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc
      421 tttaggattt agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa
      481 atggacacaa tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt
      541 agcggtaaac tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg
      601 gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa
      661 aatcactgct tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag
      721 cattaaagaa atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa
      781 agtggctggt acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg
      841 tgcaacttac tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga
      901 acaaaaagct gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag
      961 aacggatctt gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt
     1021 taatggcaag gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg
     1081 agccatcatg gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg
     1141 actagaaatt cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga
     1201 tggtacgcac atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa
     1261 aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg
     1321 tgactggcta ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga
     1381 ggacggaaca gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa
     1441 aaacatcaag ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa
     1501 tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg
     1561 ggcaggaagc tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc
     1621 agattatgaa aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca
     1681 tgcggtactt catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt
     1741 tgttcctaca aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa
     1801 caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac
     1861 ttacggtgca gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat
     1921 tgcttggagc gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt
     1981 gatcgttgat gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct
     2041 tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt
     2101 caatacagca atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct
     2161 gccttatgaa tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg
     2221 tgaagaacta tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac
     2281 atacgatgaa gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa
     2341 agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc
     2401 attagccaat gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc
     2461 ggtaccaaac aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact
     2521 gatgagttgt gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt
     2581 gttatttcaa attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca
     2641 cgttcaatgt ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt
     2701 tgtctattaa tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca
     2761 aattcataaa ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat
     2821 gaagtatgag aaaaggagag acaaatggat caagaactat ttattatcga agtattatca
     2881 gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt
     2941 ttaagcttag tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc
     3001 catatgagtg cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac
     3061 ttttcgtggg gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt
     3121 tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa
     3181 cagtttttac ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact
     3241 tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt
     3301 gatgatttac ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat
     3361 atttttttac tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac
     3421 ctgacaacta agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca
     3481 atctattatg ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac
     3541 atgacaatga actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct
     3601 gatcctaagc aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca
     3661 gagacgcact ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt
     3721 gattcgcaat ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt
     3781 tattggatgg acaacacata tgatatttcc gccgatttct catggtttat ctattcttct
     3841 ggggatgatg tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt
     3901 atcacccaat cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt
     3961 ctatttatct gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact
     4021 atggatggcg gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa
     4081 aatgtcagct tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat
     4141 acaaacaacg gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag
     4201 acagggggga tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg
     4261 ttagagacaa aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc
     4321 tgcctcttcc gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa
     4381 gtagaacaag gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa
     4441 gaaacaaatt caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag
     4501 ttgacaagag caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg
     4561 tcttattgcc gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa
     4621 gtcttttgtc actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta
     4681 ttattctttg tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac
     4741 taatcataca tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta
     4801 ttataaagac tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg
     4861 acgatatcgc cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg
     4921 tgtgcaatca caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat
     4981 gcttctgttc gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc
     5041 gctagaatga ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt
     5101 tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt
     5161 gcgatttcag ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata
     5221 ttatcagcta ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga
     5281 cgtagttctc cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta
     5341 ttcagttcat aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga
     5401 ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt
     5461 ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct
     5521 aaaagtcttg catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc
     5581 ctatcgattg ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt
     5641 ggttcagaaa tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca
     5701 cctgatagaa caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg
     5761 aaacgaatgg ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat
     5821 tgctttttat ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg
     5881 accactagat taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac
     5941 tgattctgtt cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg
     6001 ccaacgatca gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga
     6061 ccagcagcag ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact
     6121 tgagaaagtt gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg
     6181 tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat
     6241 gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt
     6301 aagactgaac agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg
     6361 aaagaaagct gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt
     6421 cccatgatca agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat
     6481 ccgtaaaaat atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg
     6541 ttcaacgttt ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa
     6601 actcctgata agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt
     6661 ttccttcatc taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa
     6721 caatcacgag agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg
     6781 ttttatagtc taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca
     6841 acgctcgagc aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag
     6901 caacaaagct gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct
     6961 tgatcgcttt agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta
     7021 aaattgtccc gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct
     7081 gttcttgagt catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt
     7141 cactaagtct caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga
     7201 tcgctgttgt ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga
     7261 tttcagtata tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg
     7321 gttcagctac tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt
     7381 ctatccgtcg taatgaggca aacgatttag ggataatgac caataccata gctgcgatca
     7441 tcaagtaagc aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta
     7501 atgctccttc tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca
     7561 tgagcaaaaa agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat
     7621 tagcatagtc agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg
     7681 cacggatcat attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt
     7741 ttggctggat actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata
     7801 cataaaaaac aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca
     7861 tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa
     7921 tcatttgctg gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa
     7981 aacgactgat atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt
     8041 ggaatccaaa cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg
     8101 ttcctagaaa cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc
     8161 cacttgctat acctgtgtcg atcagctttg ctactaattt tggtacacct aatgtaccgg
     8221 cgatttctaa acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga
     8281 atgtttttaa gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc
     8341 ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat
     8401 gaaaaaaatg tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat
     8461 ccgctattgg gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg
     8521 attgtttgat atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga
     8581 tataccgatc aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac
     8641 acttgatgaa acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa
     8701 agagataagg gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt
     8761 tcctgtagaa gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga
     8821 aatcaaaaaa tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag
     8881 ttcacagacg atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga
     8941 cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat
     9001 gaaccatgcg gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg
     9061 gaaaacagga gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag
     9121 aactgaatat tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa
     9181 ctcgaaaatg atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta
     9241 atgtggggga ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct
     9301 ttaaaaatgg aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa
     9361 agtatggaaa aataattgta ttgggtgtta tgtataattc atggtgaact gagactaatg
     9421 agaagaaaaa ttcatattgt tatgagtgag attgtatcag atgagaatga taaattaaat
     9481 aggtgcataa aagcattaca cttaaaatac attgttgctt acattattac aatatgttaa
     9541 taaggcaaaa ataaatagat ggattgaata aacatatttt ttaatcaaat agtccctact
     9601 gtgactttcc tcagctatcg gtgatactac cgatagcatt tttttgctta taaaataatt
     9661 ataagtattt agagattttt cttctaacat tcttcaaaca agaaaaatca caaaggttat
     9721 tgttattttt atatattctt tttaagaatg attgtttttt tatagaaatt ttaaaaaata
     9781 aaacacgtta ttgatggata gttagtttgt tatttggtaa tattatagaa gttgactggt
     9841 agtgatttat ttttcgtcgt tttataaaaa tgttattttt tggaaaaatt cacaaatatg
     9901 taacgaatgt ttttctggaa tttgctgtgt taaaaagcag tatgactaat ataaatacta
     9961 ttgtcattta tgcaattttt tatggatagt aattttagag gggaatcaat gaaggaagaa
    10021 tagaagggaa aaatagttgc aaatacgcca gttagcaaaa atagcaatac atcttcaagc
    10081 tttaaaattc gagagaatca tcttggtagt ggaatcaact acaacaaaag gattaaacca
    10141 attaatagat agaattacca atggaaaaat agaaaaggta gttattctat acaaaagttg
    10201 tttaattcgc ttcggttatg aacttagtga aaccctttgc aatatatggt acagaatata
    10261 gaaattattg acattacaga aaatgatgaa gatttaatac agattgtcac tatttgtaga
    10321 taccttttac aaggaaaccg agccaataaa gctaagaaga tgattaagga gttgatggaa
    10381 gacgatacaa gttaagaaag tgaaactacg cccaaataaa gagcaaaaag agtaattgga
    10441 cgtggaaaat gcaagaaatg aaccatcgat tcggtggaca gtttattagt aatgacggtt
    10501 tgagaacatc catgacgaga atgaaggaac atcctaaatg cgtttagttg aaccaagtgc
    10561 caaataatat ggccaaataa gcgatcagtt atcgatgaat cccaagtatt ctaaactata
    10621 tattcatttt gatgctaagc attgatatat ttttttggga ttgatgaagg ttaaccaata
    10681 cagggactca ctgatgaaat tctagagtct gaccttggta tcaacgattt agtgattgtt
    10741 tctaatggaa aagtataaaa gtatataagt acattaacaa aataactatt agggttacgc
    10801 tccagcttgt gcaaggcact ggcaggagga atcgtgaaat cataattatg gttaagccac
    10861 acggacaact aaaagtcata caaagctatc gaatcaatca acagaagaca atcggcaatc
    10921 attagctttg tttacagctt acattgcaac gcttgagttg aaccgaaaat acgagggagg
    10981 taacgcagcc attttgagtt ggggaatctt tttagaaaat tatcgggtac cttgttgttt
    11041 agtcaaaatt ataaggggga gtaatagtga ggaacaaatt tcataaactg ttaagtatgt
    11101 ttctagttat gcttattgtg atcaatcaat ttgggaacat aacggttcta gctgagtctt
    11161 tagcagaaca gccagagcgt tcgctgacga tcgaagttga tcaagaggag gctgctttgg
    11221 gtgagcagtt ccagttgcga gtcagaggaa caccagacct gctaaatgca ttggtcattg
    11281 ataagagtga ggatttgatt gaaggggaag tgaagcaact caatgattac gaacgtctga
    11341 ttacttattc ttcccagcat tcaggtaaat acgaattgac cgcaagaagc ggtgatattc
    11401 aatcaaatac ggtctcaata gaaatcagtg taccagaaga aagcacaagt gagtctttta
    11461 atgtaccaga agaaagcaca agtgaatctt ttaatatacc agaagaaagc ttaagtgaat
    11521 cttttaatga agccagatca tctgagccat ctataacgca ggaatctcag attgaaagac
    11581 aagaggagct cttgaattat gacatcggca tctctgctga gcgccccact ttgccgagca
    11641 ggcatacaca aacgttaaag ggatacattc aaggaggcgc tggcgatatt cttgaagaat
    11701 atggggagat ggtcgtacga gttcctagag catcgattgg cttaattgag ttcattagag
    11761 tggaggtcgc tgatgattca cctttaaagc cactggcatt ttaccaagaa ggcacagacg
    11821 ttgtttttcc atttaccttt gattatgaag gattaaatga agccgctcag tcgtatacat
    11881 tcagtatcac gtttgaagtt gctttctttc aatcaggcgg tactgagcca aataatgact
    11941 ttcaagtgag tcttacttat gctgagcgag cagcaaacac ggaattttca attacgccta
    12001 ttccttcatc aagacccatt ttttcgaaat tatactatgg ctctagagaa atcgacagcc
    12061 aaggaaatct tgggaatctg ctgattgacg ggcaagacgt gaccaacagt aataaattta
    12121 ttttgcaggt aaattatgaa ggatcacgaa aagacggtgt agtggttacc gatgtactac
    12181 cgcaaggaac aactttggca ccgaatccga ctacatggag ttccactgga aatagagaac
    12241 ctatttcagg tggggttcgg atcgcagaag taacgaatcc aggtgaagca aacgttagaa
    12301 ctcattatgt ttccgaggaa tttgtggaca aagtatcttt taataaggaa acgaatacac
    12361 ttgttgtgga ctttggtaat ctagcagagg gtcaatcgta ttttattgaa tatgctcttg
    12421 aagtgaccga tgtcgacttg ttcaatgaag gaagacaaga ggatgggttt tctcggaatc
    12481 gagcgagtat gacctacaat aatcaggtaa tgagccgaga ctaccgcttg agaatccctg
    12541 aaagtaatct tccggcaatc tctttaagaa aaagtgtgga caaaaatgtt atcaatctca
    12601 atgaaaatga actaacgtat actctgcatg tctcgatgtt ttctggaaca agaaatacgc
    12661 ttgttattga agatgttttg gaagaaggca tggaatttgt gtcatttcta tcagatattc
    12721 cctctttcat tgatgccgaa gtcaataatg gaatagttcg tttcagtgcg aataatacga
    12781 ttggtggtcc gacaagtttt accctctctt ttaaggtcaa tgtagaggcg tatgcggtcg
    12841 gcgatcaaat tttaaacttt gcaacgcttg tgaatagtga caatcgattg aatacgagta
    12901 ctgtaagaac aaggaaaatc gatgggcgta ttaaagtaat taaaactgat gagaatggcc
    12961 aaaggctggc aggagcaaaa tttgaaattt tatcaagcga ttcacaagtg atacaagaag
    13021 gtataacggc agaaaacgga gaatttttaa gtgaaccgct tcctgtaggt agctatcaag
    13081 tccgagaaac agaagcaccg gaagggtatc ttttagatgc gacggtacat gatgttttta
    13141 taaatgaata ctccatagaa gaagtgacgt taaccattga aaacatcctt gatacgggtg
    13201 gtgttgaact aattaaacat gctgaaggca gtggtgaagt ccttcaagga gctgtttttg
    13261 agttacagaa ccgagaagga gaaacgctac aaaccggttt aactacagga gaagatggaa
    13321 aactggcaat tgacggttta gcaccaggag catatcagct ggtagaaac
//
LOCUS       DAZAJZ010000078        13311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000078 DAZAJZ010000000
VERSION     DAZAJZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.078"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..642)
                     /locus_tag="ACG86D_002193"
     CDS             complement(13..642)
                     /locus_tag="ACG86D_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3141321.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(776..848)
                     /locus_tag="ACG86D_002194"
     tRNA            complement(776..848)
                     /locus_tag="ACG86D_002194"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(813..815),aa:Thr,seq:ggt)
     gene            1062..1135
                     /locus_tag="ACG86D_002195"
     tRNA            1062..1135
                     /locus_tag="ACG86D_002195"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1096..1098,aa:Asn,seq:gtt)
     gene            complement(1273..1437)
                     /locus_tag="ACG86D_002196"
     CDS             complement(1273..1437)
                     /locus_tag="ACG86D_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141322.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            1732..3060
                     /locus_tag="ACG86D_002197"
     CDS             1732..3060
                     /locus_tag="ACG86D_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN3141323.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3262..4095
                     /locus_tag="ACG86D_002198"
     CDS             3262..4095
                     /locus_tag="ACG86D_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN3141324.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4111..4848
                     /locus_tag="ACG86D_002199"
     CDS             4111..4848
                     /locus_tag="ACG86D_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN3141325.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5003..5971)
                     /locus_tag="ACG86D_002200"
     CDS             complement(5003..5971)
                     /locus_tag="ACG86D_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN3141326.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6203..7036
                     /locus_tag="ACG86D_002201"
     CDS             6203..7036
                     /locus_tag="ACG86D_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN3141327.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7070..7909
                     /locus_tag="ACG86D_002202"
     CDS             7070..7909
                     /locus_tag="ACG86D_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3141328.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            7935..8495
                     /locus_tag="ACG86D_002203"
     CDS             7935..8495
                     /locus_tag="ACG86D_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3141329.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            8694..10415
                     /locus_tag="ACG86D_002204"
     CDS             8694..10415
                     /locus_tag="ACG86D_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN3141330.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            10580..11722
                     /locus_tag="ACG86D_002205"
     CDS             10580..11722
                     /locus_tag="ACG86D_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN3141331.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            11738..12949
                     /gene="thiI"
                     /locus_tag="ACG86D_002206"
     CDS             11738..12949
                     /gene="thiI"
                     /locus_tag="ACG86D_002206"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN3141332.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4533 a         2214 c         2729 g         3835 t
ORIGIN      
        1 tcttttttta aattaactat tttcgataaa agatcgcata gcttgtgcca ttatttctgg
       61 cttttctcta ttcaattcat gttttccgtt tttaataaag agtagccggc cattcgttga
      121 aagtttatat aaacgttttg ctgctggttt attcgcatga tctttggtac cgcataaaat
      181 caatttagga agtcgtgatg tacgaagctg ttttttgcag tctaatgttt caagtgaacg
      241 aagtagggta agggtttgtt ttttagatag acctaatttt tcaaatatct tagaaggtac
      301 gcagtaaaaa taaatattct ggaaagtaag caataagcga ttaggagact caaattgtgg
      361 agcgactaaa acaacctttt ttaaatatgg agaaggagtt gcaagatagt ttaaagcaat
      421 aacagcgcct aaagataatc cacataaaat aaaaggttcc tttatctgat tcaattgtac
      481 ttcgatgtat ttggttaagt cgtctaaagt agcagtgtct gtgctgttta aatgattgaa
      541 caatgaaaga cttttgcaag aataactatt gggtagataa gagattacat ctttccatga
      601 ttgttcgtct tggcctagac catgcaaaaa aacaattttc atattgctct cctttttata
      661 gttatcatat agactttctt tgtactatta aaatataaac attcatttct tacgtcataa
      721 aataaaaaaa cagcgcagac acattgttaa accaacatgt tgcactgttt aaggaagcca
      781 cttgccgggc ttgaaccggc gacctcttcc ttaccatgga agcgctctac cagctgagct
      841 aaagcggcat tatcattaat acaaataata ggataacgaa tattaatgta tttgtcaaca
      901 gaattttttc gaaaaaatgt gtttctctaa taggaaaaca gattttagaa aaaataaaaa
      961 taacaaaaaa tgcgaacggt ttctttgata atataataaa aaaattcaaa aaaagtattg
     1021 cgttatttag aagaaattga tattatattc atgtacttga ttccggcata gctcagttgg
     1081 tagtagcgca tgactgttaa tcatgatgtc gtaggttcga gtcctactgc cggagtaaat
     1141 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
     1201 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
     1261 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
     1321 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
     1381 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
     1441 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
     1501 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
     1561 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
     1621 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
     1681 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
     1741 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
     1801 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
     1861 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
     1921 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
     1981 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
     2041 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
     2101 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     2161 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     2221 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     2281 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     2341 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     2401 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     2461 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     2521 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     2581 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     2641 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     2701 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     2761 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     2821 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     2881 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     2941 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     3001 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     3061 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     3121 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     3181 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     3241 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     3301 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     3361 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     3421 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     3481 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     3541 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     3601 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     3661 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     3721 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     3781 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     3841 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     3901 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     3961 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     4021 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     4081 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     4141 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     4201 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     4261 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     4321 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     4381 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     4441 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     4501 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     4561 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     4621 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     4681 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     4741 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     4801 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     4861 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     4921 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     4981 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     5041 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     5101 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     5161 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     5221 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     5281 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     5341 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     5401 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     5461 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     5521 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     5581 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     5641 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     5701 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     5761 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     5821 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     5881 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     5941 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     6001 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     6061 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     6121 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     6181 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     6241 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     6301 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     6361 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     6421 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     6481 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     6541 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     6601 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     6661 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     6721 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     6781 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     6841 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     6901 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     6961 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     7021 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     7081 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     7141 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     7201 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     7261 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     7321 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     7381 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     7441 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     7501 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     7561 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     7621 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     7681 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     7741 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     7801 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     7861 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     7921 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     7981 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     8041 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     8101 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     8161 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     8221 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     8281 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     8341 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     8401 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     8461 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     8521 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     8581 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     8641 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     8701 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     8761 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     8821 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     8881 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     8941 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     9001 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     9061 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     9121 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     9181 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     9241 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     9301 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     9361 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     9421 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     9481 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     9541 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     9601 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     9661 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     9721 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     9781 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     9841 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     9901 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     9961 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
    10021 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
    10081 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
    10141 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
    10201 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
    10261 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
    10321 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
    10381 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
    10441 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
    10501 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
    10561 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
    10621 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
    10681 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
    10741 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
    10801 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
    10861 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
    10921 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
    10981 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
    11041 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
    11101 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    11161 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    11221 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    11281 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    11341 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    11401 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    11461 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    11521 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    11581 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    11641 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    11701 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    11761 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    11821 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    11881 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    11941 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    12001 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    12061 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    12121 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    12181 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    12241 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    12301 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    12361 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    12421 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    12481 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    12541 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    12601 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    12661 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    12721 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    12781 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    12841 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    12901 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    12961 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    13021 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    13081 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    13141 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    13201 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    13261 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAZAJZ010000079        13086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000079 DAZAJZ010000000
VERSION     DAZAJZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.080"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG86D_002207"
     CDS             complement(79..2199)
                     /locus_tag="ACG86D_002207"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3141333.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG86D_002208"
     CDS             2429..3607
                     /locus_tag="ACG86D_002208"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN3141334.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG86D_002209"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG86D_002209"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN3141335.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG86D_002210"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG86D_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN3141336.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG86D_002211"
     CDS             complement(4960..6213)
                     /locus_tag="ACG86D_002211"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3141337.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG86D_002212"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG86D_002212"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN3141338.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG86D_002213"
     CDS             7790..9136
                     /locus_tag="ACG86D_002213"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN3141339.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG86D_002214"
     CDS             complement(9444..9830)
                     /locus_tag="ACG86D_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3141340.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG86D_002215"
     CDS             complement(10098..10247)
                     /locus_tag="ACG86D_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141341.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG86D_002216"
     CDS             10709..10921
                     /locus_tag="ACG86D_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141342.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG86D_002217"
     CDS             complement(11199..12998)
                     /locus_tag="ACG86D_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN3141343.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAZAJZ010000080        12830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000080 DAZAJZ010000000
VERSION     DAZAJZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.081"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..480
                     /locus_tag="ACG86D_002218"
     CDS             <1..480
                     /locus_tag="ACG86D_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141344.1"
                     /translation="YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQL
                     LLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATV
                     LKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(747..1916)
                     /locus_tag="ACG86D_002219"
     CDS             complement(747..1916)
                     /locus_tag="ACG86D_002219"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3141345.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2120..3295
                     /gene="mltG"
                     /locus_tag="ACG86D_002220"
     CDS             2120..3295
                     /gene="mltG"
                     /locus_tag="ACG86D_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN3141346.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3431..3910
                     /gene="greA"
                     /locus_tag="ACG86D_002221"
     CDS             3431..3910
                     /gene="greA"
                     /locus_tag="ACG86D_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN3141347.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4079..4810
                     /gene="liaF"
                     /locus_tag="ACG86D_002222"
     CDS             4079..4810
                     /gene="liaF"
                     /locus_tag="ACG86D_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN3141348.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4807..5874
                     /gene="liaS"
                     /locus_tag="ACG86D_002223"
     CDS             4807..5874
                     /gene="liaS"
                     /locus_tag="ACG86D_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN3141349.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5881..6513
                     /locus_tag="ACG86D_002224"
     CDS             5881..6513
                     /locus_tag="ACG86D_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3141350.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6653..7318
                     /locus_tag="ACG86D_002225"
     CDS             6653..7318
                     /locus_tag="ACG86D_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN3141351.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7346..7639
                     /locus_tag="ACG86D_002226"
     CDS             7346..7639
                     /locus_tag="ACG86D_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN3141352.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(7707..8654)
                     /locus_tag="ACG86D_002227"
     CDS             complement(7707..8654)
                     /locus_tag="ACG86D_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN3141353.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8832..9635)
                     /locus_tag="ACG86D_002228"
     CDS             complement(8832..9635)
                     /locus_tag="ACG86D_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN3141354.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9781..10695
                     /locus_tag="ACG86D_002229"
     CDS             9781..10695
                     /locus_tag="ACG86D_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3141355.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10743..11213
                     /locus_tag="ACG86D_002230"
     CDS             10743..11213
                     /locus_tag="ACG86D_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN3141356.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11298..11906
                     /locus_tag="ACG86D_002231"
     CDS             11298..11906
                     /locus_tag="ACG86D_002231"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN3141357.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12040..12240
                     /locus_tag="ACG86D_002232"
     CDS             12040..12240
                     /locus_tag="ACG86D_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3141358.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4345 a         1956 c         2551 g         3978 t
ORIGIN      
        1 tattcatcag tagttaacat ggtgaaagaa atgaattttc aaggaactta ttctaatgaa
       61 caaatggaaa ataaacttga agaatttgag aattttgatt atgtacctat aggctataaa
      121 aaagatcaat tgcttttgaa aaaccatcaa gttacagaag atggtcaagt tatttatagt
      181 agcttttctc caaataaaga tgaaattaca tttacgttta aatcttcaaa aggcaaaaca
      241 atagttttac ctgtattccg gtataaagga ttaacggtta ctgttaattc aaaaaatgct
      301 acagtactaa aacaaggagg tccttttgtt acagtaaaaa gtgaaagtgg aataaataaa
      361 gtaacaatca ggtattcata cacatttttt tctaaaatct cattatttat ttctataatc
      421 acgctaattg ttcttctttt ttattgcata aaattaaaaa taagaacaag ccatgaatga
      481 tttgctagca catattttag caatctaaag atactatttt agatttaata ggccctttgt
      541 caaataatgt tggtaaagag aaatcttaga ataatttgga cagaacagaa agaagaacac
      601 ctctttctgt tctgtttatt tggaattaaa gtctgtagca aaaattgatt tttagatttg
      661 tcccacactc tttcctacgt ttagtcgagt acttttttta agcaaatgct attcaaagaa
      721 gtagtatttg ctatcttttc atcatatcag ctatttgcca acttagtagt tccttccatc
      781 aggtcataga tttggtttct tagatcagca gtaggcagtt tgatagtttt gtcagctttt
      841 atattgcttt ccatgatgat agcatcctgt gaatctttgc taaatatagc agatgtatca
      901 aaagagtcga tatttccttg cgacaggata taattatctc cgctatatac cccaccacta
      961 tatggatgtc cattcgcatt ttcacgccat aatgagaaga gtaagtcttt ggaaactagt
     1021 tttccagata aaacttgtgt aaacagcgta tttaaatcat agacggacat actgatatta
     1081 ccagtcccta attcgttatg gatctctgtt tcagattctg tcagtggttt aaagtaatca
     1141 tcctctttag acatttgata ggatagtgca tgattggatg aatttttgac ttggtcataa
     1201 aattttgtat gctgtaatcc taaaggttcg atgatatcat cggtcaataa tttttcatac
     1261 ggttgatttg ttaatttgat caaaattcca gtcaatatgg taaagttcaa tgaatcataa
     1321 tgagaggtct gataatctgt aaattttaag tgctgaatcg caaattgaac gacttcttca
     1381 tcagtcatcg gtatggttgg agaagcagtt cggtgcaagc ctgagcgcat atacagcaag
     1441 tcacggatag taatttgctg gctgccagca atttgtggat agaattgagc cagcttagta
     1501 tctaaagaaa ggtgcccacg ctcgacttgt tttaaaatca aaagagtagt caatgctttt
     1561 tgaaccgaac caatctgata ataaagagaa ggatcatttg gttgttgcgt gagtttattc
     1621 gcatagccat agcctttttc catgatgatg cgattatttt tctttacgta aattgagcca
     1681 atataatcat tttttcttaa tagttgatcg attttttcaa cgagctgttg ttcattggta
     1741 gtcagtatca actttttttg agctgattga gcatgggcaa ctatctgaga gtgttcatta
     1801 ttgtaaaatt gccatccaag aatagttcct gtagagaacg cactaaggct aaacaacacg
     1861 acaacgatca ttaaaaatga atattttctt tttttagcat gttttccttt tttcataata
     1921 gtttatttag tctcctttct tttttacaga aagaataaag ctaaaatagc ttgtatcttg
     1981 ataacattta ttttaatctt atagtaatgg atttactact tacatttttg atagaatatt
     2041 taaataaaat cattcacgcc aattcaaata tcatgtataa tgaataaagt tacgaacttg
     2101 atagtaagga ggagttgttt tgtcaggaaa caaacaacac ccaagtaaag ataaaaaaga
     2161 agccagtaag gcagctacag cagatataat cgacaagcgg agaaatctgc gtaaaaaaga
     2221 agataagatt gtgaggaaga tcattttagt gattgctctt acattattga ttattggcgg
     2281 atttcttgga tttaccgtgt atcgttatgt agatagcggg ttgaagcctt tagataaatc
     2341 agatgaccaa ttagtccaag tcgagatccc gagtggctct tctaacaagc agattggtga
     2401 gattttggag aaggacaata ttattaaaag cgggattgtt ttcaattatt atacaaaatt
     2461 taaaaattta actggctttc aagctggcta ttatcagtta gcgcctaata tgactcttga
     2521 cgagattggc aaacagttgc aagaaggcgg tacttctgag ccaacaaaag ttgcagatgg
     2581 gaaaattgcc attccagaag ggtacgatat cgatcagatt gctgaacggg tagcgaaggt
     2641 aactggtaaa gacaagaaag aatttttaga tttagtgaat gatgagactt tctttaacga
     2701 gttgcaccaa aaattcccag aactgttgga aagtgcatct aaagctgaga acacaaaata
     2761 ccgtttggaa ggttacttat tcccagccac atatgattat tacacgagta cttcgcttaa
     2821 ggatctagtg attgaaatgg tgaacaagac gaatacggtc atgcaaaact attattcagc
     2881 tatcaagcag aaaaatttga ctgtacaaga agttttaact ctagcttctc tagttgaaaa
     2941 agaaggcgta aaagaaaatg atcgtaaaaa cattgcacaa gtcttcttta accgaatcaa
     3001 agcaaatatg ccacttcagt cagatatctc tgttttatac gcattaggtg aacacaaaga
     3061 attagtgacg tatgaagata cagctgtaga ttcaccatat aatttgtata ccaataccgg
     3121 ctatggacct ggaccttttg acaatccaag cgaagaggca atcaaggcag tattagaacc
     3181 agcagaaaat gactattatt atttcgtagc agatacgagc actggaaatg tttatttcgc
     3241 taaaacttat gaagaacata tggaattagt ggaaaaatat gtaaataatt cttagaaaaa
     3301 caggctctag acattgatca tttttagaaa aacatttaca tttgaagaaa agcgtggtaa
     3361 tcttttttgg actatattcc tcaaaagatt accttttgcg gtattttatc gaaaaaagga
     3421 gacgttacac atggtagaga aagtttatcc aatgactcta gaaggtaaag aaaaattaga
     3481 aaaagaatta gaagaactaa aaacagtcaa acgcggagaa atcattgaac gaatcaaaat
     3541 tgcccgcggt ttcggagatc tttccgaaaa ctctgaatat gaatcagcga aagatgaaca
     3601 agcatttgtt gaaggaagaa tcacaacaat tgaaaatatg attcgttttg ctcaaatcat
     3661 cgataacgac ggtgtagatg cagatgaggt atcaattggt aaaacagtta cattcatcga
     3721 actaccagat ggagaagaag aagagtacac gattgtcgga agcgctgaag cagatccgtt
     3781 ttctggcaaa atttccaatg attctcctat tgcgcaagca ttgattggca aacgtaaaaa
     3841 tgatgaagta actattgcta ctccaggtgg cgatatgtta gttaaaatcg tcaaagtaga
     3901 acaagcgtag tctgactgaa agatctagag agttcatctt actgatgata cactctagat
     3961 ctttttctct ttgtgtattt tagatttcaa caaacgatct tacatctaaa gtcctatagc
     4021 caacagttaa aaaaataggt atgatgaaat gagaatgtgt caggagggag aaagagcgat
     4081 gaatagttct tggcgttttt ttgtggtagt tgaagcacta ctgctgttat ttgctgtttg
     4141 gcaaatcgta aacaatactg gacttttgct tttagtgata ttcggtattt tcaatatata
     4201 tctagctatg cgcaaacatc ctcgtactaa atttcaaaat tttcaattga tattaggaag
     4261 tttggtcatt ttttttagct tagtcaacag tcctgcttta tggatgatgg ctgtatttgc
     4321 cgttttattt attggtctga aaggtgtaga gatttcaggg atagatttaa ccaaaaatgc
     4381 tttttggcga aagaaacaga tcatgatgat ccaaacagag caattaaaaa cacataataa
     4441 tgagcggaaa agacagcaat tattcggtaa ccaacgtatt ggaaacgacg tctatgaatg
     4501 ggacgatatc aacatcgccg tcatttctgg tgatactatc atagatttgg gcaatacgct
     4561 tttgccaaaa gatgataata ttgtgattgt aagaaaaggg ataggcagaa cacggatctt
     4621 agtacctttg ggcgtagcca tccgattaga gcatgcgaca ttagtaggaa atgtcttgtt
     4681 tgaagaagag caattttctt tgaaaaatga acaaatcaaa atctacagca gtgattatga
     4741 tgaaaaccct cgtagattga agattattac taataccttg attggagatg tcgaggtgat
     4801 acgaatatga tgggaaaaat atccagagcg atgctagctg tttattcggg gattgcggct
     4861 ttccttatta ttctattttc acttttcact tatttttatg ccagcaatca aagtcattgg
     4921 tggggagaat tactgcgtgc acgtttatta tatgttccgc ttatcttcca tttgttagcc
     4981 atttccttag gtgtaggatt aattgtcttt ctattattat cacttattca aaagacaaaa
     5041 tacgggaaaa tcgaagaaaa gctgcgtgca ctttcttctg gcaattatga atccaaattg
     5101 ttgcttcttc cgatcccaag tgcatcggac gatttataca tcaaagatat tgataaggaa
     5161 atcactaaga taaaagaaaa aatgattgaa gtatctagtg aattacaaat tgtaacgagc
     5221 cgtccccaat atgtagatgg acaaaccaaa gaagaaattt tagaattaga aagacatcga
     5281 ttagcccgtg agctgcatga ttcagtttcg caacaattgt ttgcagcaat gatgatgatg
     5341 tcggcattga cggagcaagc agaaaaaagc gagacaccag aaatgtttcg caagcagttg
     5401 aaaatggtag cagagatcat caacgcttcc cagtctgaga tgcgtgcgct gcttcttcat
     5461 ctacgtccag tcaatttaga agagaaaagt ttaaagcaag gtatcgagca gttattgaag
     5521 gaattgcaaa ataaaatcca gatttcgctg aaatgggatg tagaagatgt aaaactatct
     5581 agttccattg aggatcactt gttccgaatc gttcaagaat tgttatcaaa tacattaaga
     5641 catgctaaag ccaatgaatt agaggtatat ttgcacaaaa tagacaataa tcttttgtta
     5701 cgtattattg acgacggaac agggtttaat atgaatgaaa cgaaaacggg aagttatgga
     5761 ttgaacaata tcaaagaacg agtagctggt attggtggaa cagtaaaaat cattagtttt
     5821 aaaggtcaag gtacaagtgt agaaatcaag gtccctttga tgaaggaggc atagataagg
     5881 atgataaaag ttttattagt agatgaccat gaaatggtgc gcttaggcgt ctctttttat
     5941 ttatctattc aagaagacat tgaagtaatt ggagaagctg aaaatggacg acaaggctat
     6001 gagaaggcga tgacacttcg accggacgtc attttaatgg acttagtcat ggaagaaatg
     6061 gatggtatcg aatcaacaaa agcaatctta aaagattggc cagaagctaa aatcattatc
     6121 gtaaccagtt ttattgatga cgaaaaagtt tatcctgcca ttgaagcagg tgcagcaggg
     6181 tatttgttga agacctctac cgcacatgaa attgctgatg ctataagggc aacgcaacgt
     6241 ggcgagcgag tattagaacc agaagtgaca acaaaaatga tggaaaaaat gagtcggcga
     6301 aatgagcctg tattacatga agaactaacg aatcgggaaa atgaaatttt aatgctgatt
     6361 tctgaaggaa aaagtaatca ggaaatagcg gacgaattgt ttattacact gaaaacagta
     6421 aaaacacatg tttccaatat attggctaag ctagaagtgg aagaccgtac acaagccgct
     6481 atctatgcat tcaaacatgg tttggtgaaa taaaaacgat gataaagagg ataatcaaaa
     6541 gctgagaaaa taactattat ttttcttggc ttttttacaa taatgatgct tgcttttgtt
     6601 tttgtaaagg ctttgttata ctgagaaaag aagatactaa aggaggaaaa cggtgaaaca
     6661 aaattttgca attgtaggtc ttggacgatt tggcggaagt atctgccgaa cactgatcga
     6721 agctggtcaa gaagtactgg ctattgatag taatgaagat cgggtcaatg aatatatgaa
     6781 tatagccact catgctgttg tggcaaatgc acaggatgaa atgacacttc gatcattagg
     6841 cataagaaat ttcgatcatg taattgtcgc tattggagaa gatattcaag ccagtatctt
     6901 agttactctg atggtaaagg aaatgggcgt accaaatatt ttagcaaaag cccaaaatga
     6961 gtatcatgcc agagtacttg aaaagatcgg agctgatcgc gtcgtccatc ccgagcgaga
     7021 catgggaata aggattgcgc ataatttagt gtcaaagaat attttagatt acctagagtt
     7081 gtcagataaa tattctttag cagaaatccg agtctccaat cctaaatttt tcaacaaaac
     7141 attagctgaa ttaaatttca ggcaacgttt tgacttaaca gtagtcgcca tacgaagagt
     7201 agatcaaacg gttatcgctt ctcctgcagc agatgaatat gttcgtgaaa atgataatct
     7261 actggttatt ggagagacag gcgatgtgga tattctagac gataagatga atcaatagaa
     7321 agaaaagaga aagtaggttg ttgggatgaa attaacggta acgccaaaag cacaaaagtg
     7381 gtttgactca gaaattgatc tgccagaaac ttacggaata cgtttttttg gaaaagtcta
     7441 cggcaaaaca gaggttcatg atggtttttc gattggaatg tcagttgaac agccagaaca
     7501 tcctgtaaag aaagtggaaa ttgatgggac actttttttt atcgaggaag cagatgaatg
     7561 gttctttaaa ggatatgatt tacttgttga ttataatccc aacctggagg agccaaccta
     7621 tcactttaaa aaacaataaa aaaagagagg gtgacacaat ttttgtcacc ttctcttttt
     7681 tcttttgaaa atattgtttt cagctattaa tatcctgcag acaattttac ttgattggtt
     7741 tgaagctcat gggtggcaaa gtagcttttc agattggtta aaaaaatagc cattaatttc
     7801 ttttgaaaat caggtgtcaa acctgcaatg tgggaagtaa tcagtacatt gtccattttc
     7861 caaagaggat gatcttgtgg aagaggctct tcctcaaaaa cgtctaatgc tgcaaaggaa
     7921 agttgctggt tatttaaagc ctgcaccaga tcttgtgtct taacagaagc accacggcca
     7981 acattgatga atacagattt tggatcaaag tgttcaaaca tatgctgatc aaagagccca
     8041 gttgtttcct ccgttagagg taagatattg actacgaaat ctacttcagg aagaacttcc
     8101 tttagttgat ttaaagaaaa agtcttttcc attccttctg ctggatgacc ggatgtattg
     8161 attccatagc agttcatgtc taatgctttg catttttttg caatcatttt tccaatatgg
     8221 ccagttccaa tgattagtat ttttttccct tggatagatt ggtaatggac cgatgaacct
     8281 aaccatttca tgtcactctg tgccttttgt gcttgaaaaa gaccacgaga atacccaagg
     8341 atgattccta agacatgctc cgtaatcgat tcactgtgga tgccactacc atttgaaagt
     8401 aaaatatgtt tctttgaaaa agtctcaagg ggtagctgat caacaccagc tgagatagat
     8461 tggacccatt tcaaggtaga agaggctaat agtttttctt ggtagtctga gttccagccc
     8521 aaactgatct ctacattttg tagatcctct ggttgtaatt ctgttttaaa gatataacca
     8581 ggcgctatag tatggatttc ttcgataaat tcatcacgaa atggacgatc tgcataaatg
     8641 attctttctg acatagttaa tttcccgcct ttctttactc taagtgtagc gcatacaaga
     8701 agaactttga agcgccttgc ttgttttttg agaaagaaat atttttcaaa taaaaaaggg
     8761 gctgtgacaa aaaaatgtca caacctctaa aagggtctat tgaagtagaa ataacgagtt
     8821 gtctttattt cttaattgat cgatattttt gcccatcctt tgacagcttg agcaaaaata
     8881 aagattcctg caaaagcaat gaaggcgcta gtgtcaaaag aaggaagaaa gaccatcaag
     8941 attgttgcaa ttatagtagg caatgaaatt gaatagatca atgtttttaa acaatcaaga
     9001 aaagtagctt ttgttagtcg aaagcgtgca tagagaaaag cagcaatgtt ggtaaatagc
     9061 aaagtgatca gcagattcag aaaactagga taaatagaaa atataaatgt caggactttt
     9121 atccaaagtg gtatagatgc ttcacttaaa aaagaacgga gctgcttacc tgttaagttt
     9181 tttaaagatt cattggtgta gggaagttct aattggttat tgttcaacaa agcagaagtc
     9241 gtgcctgtat taggaagagc tatgataagt ttgtttttta acatcccgac actaacaaaa
     9301 ttccccatca aatcagtaga gatgtcctgt tctgttcgtt ttccctctgg atcaaatgtg
     9361 aagataatgg agtttgtttg gtaaatgaat ccttcttttt cttcggtttg aagctgcccg
     9421 ttttggattg taaaatcagg aatgcgagta gcaatctttt gcccatccaa ttgaatgttg
     9481 tttaagactt gaaaaacttg aaaagagatt ggcaaagcca taattatact caaaactaaa
     9541 agatagacaa tggctttcca aaatgggaca tttctcgcct cctttaggtc tttgaatcgt
     9601 ataaaggaac tgataattaa ttgttttgtt gtcataagta ctcctcatct agtagtgtca
     9661 tcacttgcaa ttctagcgaa gtatcaagtg aaatacaagt gctttaagga aacagctgaa
     9721 tgattggtga aatatcaaaa aaatgttatt cttgtctaaa gactatccaa tataaataaa
     9781 atggaggaag gaatagtgcc tacacagcca cagagatcat caaaaaaact atttggagta
     9841 gtggttttga atgcggtgat cactttatca gaatttattg gagggattat gtcagggagt
     9901 ttgtctcttg tttcagatgc tttccataac cttagtgata cattggcaat tattttttct
     9961 tacggggcac aaaaaatggc ccgaaaagaa gcaaataaaa aacgtacgta tggctaccag
    10021 cgtttagaaa tattgagtgc tttcatcaac tcattcatat tgatcatatt atccttgttt
    10081 ttaaccgttg aagcattcaa gcggttcact tcaccagaaa agataaatag tcatttgatg
    10141 ctaacagtag cagttattgg attgctcgcg aatttgttct cagtattatt attacgacaa
    10201 gaagcagatg aaagtttgaa tataaaatct agttatttgc atttgttaag tgatacactg
    10261 tcttcggtaa gtgtgattat cggagcaatt ttgatccgtt ttttcgatat ctactggatc
    10321 gatccagtta ttacactgat catttcgata tatatattga tagaagcgat aatggtgatc
    10381 aaaaaagctg ccgctatttt gatacaaagt gcacctgcta tcgactatga aaaaatggaa
    10441 caagaaattg aagcaatcga tggggtaaag gatgttcatc atgtccatat ttggcagtat
    10501 agcgaaaaga taattatttt cgacggacat attgattttg aagatcagct actaagcgag
    10561 attgaaaaga tatatcctcg tataatttct ttattaaaat taaaatatgg tattacacat
    10621 gtgacgatcc aagcagagac aaaggcaaag gatcaaaaaa aattaatttt tttaaataaa
    10681 gatgaaagtt attgaaatat agagtatgat aaataagaat ttttaataag gtaggttggc
    10741 tcatgaagca gtatcgacag ttaaaaggat atttagaagc aaaaggatat tgggagatac
    10801 tagtctatct cttttttggt ggtttggcta cgattgtaaa ttttgttagt tttgctctcg
    10861 cacgccaata ttttgattta agtatggcct tatccaattc tatttcgtgg ttttgttctg
    10921 tattgtttgc ttttgtaacg aacaaacttt gggtattcca ttcaaaatca ccgaatttta
    10981 cacatgcact tattgaatgc ggtaagttct tcttctaccg tatcctttct tacggattgg
    11041 acatgggggc aatggtattg ttgatcaatg ttatgaacag caatgagtat gtggcgaaaa
    11101 tcatcactca aattattgtt atccttgcga actatatttt cagcaagttg tttattttta
    11161 aagaaactga ggtttttgaa gaagaagtag gtcaaagtga aaaagacgac taggcataac
    11221 aattgaagaa tttccttctc tttgttactc tttgtaatgt agacattatt aagaaaacat
    11281 tgaggaggaa tttttttatg acttacacat taccagatct accttatgca tatgacgcat
    11341 tggaacctta tattgacgaa gaaacgatgc atctgcatca tgataagcat cacaatactt
    11401 atgtgacgaa tttaaattca gcaattgaga aatacccaga attaggcgaa aaaacaatag
    11461 aagaattatt atctgatatg gacgctattc caacagatat caagacagct gtacgtaaca
    11521 atggtggcgg acatgctaac cattcatttt tctgggaaat catggcacca aatgctggtg
    11581 gcgaacctac aggagaaata aaagaagcga ttaatgaagc ttttggtgat ttttcttctt
    11641 ttaaagaaga attcaaaaaa gcagccgctg gacgatttgg ttctggatgg gcttggcttg
    11701 taatggaaaa tggaaaatta gctattacct ctactgcaaa tcaagattct ccattgatgg
    11761 aaggaaagac accaattcta ggtttggacg tttgggaaca tgcttattat ttgaaatata
    11821 aaaatgtccg accagattat attgcagcat tctggaatgt tattaattgg gatgaagtaa
    11881 acaaacgttt cgaagcagca aaataaccat ttcctccgtc atatcagtct taaaaataat
    11941 ttggaaaata aaagagcgaa agcttgctag cgttttctaa attgggtata atgttcttgc
    12001 aaggttactt gcaagaactt ttttggagga tatacattta tggaacacgg aacggttaaa
    12061 tggttcaaca atgaaaaagg atttgggttt attacagtag acggcggaga tgacgtattt
    12121 gttcatttct cagcaattca aggagacggc ttcaagagcc tagaagaagg tcaagaagta
    12181 gaattcacga ttgttgaagg tgcacgcgga cctcaagctg ctgaagtgac taaattataa
    12241 taatttgttg aataaagaca cgacttctta gagagtcgtg tctttttgtc tatttatgaa
    12301 gtttttagat tttttggcta agtaaatgca tttgttcact tgacatgtcc taactaatta
    12361 tcaagcaagg aacattttct tcgttagtaa tggattttaa agaaatttca gtttttggat
    12421 gttgaataaa agaccatttg tgaaaccact agtatacata ctcattacca tgtcaattct
    12481 attactttaa aagaaaatcg ttttactttg aagaggagca tatctatcca ttaaaagatg
    12541 atggtaatgc aatcactagt tcgagtcatt ttaagagcca tgcagtgagc tggaaataaa
    12601 acgaatagaa gatgggttta acaaaaggac cagtcatagg aagaaattgt cgtgaaatcg
    12661 aatgataatc tgaacggtag gcaaagactg ttaaaaagat gggaaaggta tgtgcgatca
    12721 ttttactgca cgattatttg gaaaatacct tgttgttcgc tttttctgga aaaaatacat
    12781 aaaaaaagat aaaacgattg cgaactgcgt gtattcggtc tttcgatctt
//
LOCUS       DAZAJZ010000081        12746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000081 DAZAJZ010000000
VERSION     DAZAJZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.082"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..719)
                     /locus_tag="ACG86D_002233"
     CDS             complement(114..719)
                     /locus_tag="ACG86D_002233"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3141359.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            915..1838
                     /gene="coaA"
                     /locus_tag="ACG86D_002234"
     CDS             915..1838
                     /gene="coaA"
                     /locus_tag="ACG86D_002234"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN3141360.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            1897..3462
                     /gene="guaA"
                     /locus_tag="ACG86D_002235"
     CDS             1897..3462
                     /gene="guaA"
                     /locus_tag="ACG86D_002235"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN3141361.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3953..5584)
                     /locus_tag="ACG86D_002236"
     CDS             complement(3953..5584)
                     /locus_tag="ACG86D_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN3141362.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5598..7271)
                     /locus_tag="ACG86D_002237"
     CDS             complement(5598..7271)
                     /locus_tag="ACG86D_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN3141363.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7284..7601)
                     /locus_tag="ACG86D_002238"
     CDS             complement(7284..7601)
                     /locus_tag="ACG86D_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3141364.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7626..9026)
                     /gene="lacG"
                     /locus_tag="ACG86D_002239"
     CDS             complement(7626..9026)
                     /gene="lacG"
                     /locus_tag="ACG86D_002239"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN3141365.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9263..10048
                     /locus_tag="ACG86D_002240"
     CDS             9263..10048
                     /locus_tag="ACG86D_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3141366.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10051..10743
                     /locus_tag="ACG86D_002241"
     CDS             10051..10743
                     /locus_tag="ACG86D_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3141367.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11017..11388)
                     /locus_tag="ACG86D_002242"
     CDS             complement(11017..11388)
                     /locus_tag="ACG86D_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN3141368.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(11788..12018)
                     /locus_tag="ACG86D_002243"
     CDS             complement(11788..12018)
                     /locus_tag="ACG86D_002243"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN3141369.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(12112..12450)
                     /locus_tag="ACG86D_002244"
     CDS             complement(12112..12450)
                     /locus_tag="ACG86D_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141370.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT         3909 a         2707 c         2235 g         3895 t
ORIGIN      
        1 aagaaaaaat agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc
       61 cgtacatgca cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct
      121 ttcacacttt tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat
      181 gtttcttcca ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc
      241 agtgtaccgc ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt
      301 tttccagcac ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct
      361 tcatagatat tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct
      421 tgcgctagac ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca
      481 aatgccaaag ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca
      541 tcaggtaact cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt
      601 tcacgtgaaa atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa
      661 gcccattgtt caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg
      721 tcttccatcc ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca
      781 agtcaagaat aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc
      841 tctcttaagt ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa
      901 cgcaggaggc taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc
      961 gagaatttta tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa
     1021 gtttgaatga ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc
     1081 tgatttatct ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc
     1141 ataaatacgt agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa
     1201 aaagtacaac agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg
     1261 ttcagctgat cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa
     1321 tcatggaccg taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga
     1381 accgtgtaaa atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg
     1441 atatcattcc agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga
     1501 tcaatacgct tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt
     1561 ctgtctatgt cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg
     1621 cgttactgga tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag
     1681 atcgtgaaga agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt
     1741 tacaggaata tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa
     1801 atcatatcat cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat
     1861 tttgagataa aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg
     1921 aacgttgaaa agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga
     1981 cgtattcgtg atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa
     2041 gtaaaagcga tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat
     2101 gagaatgcat ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt
     2161 tgctatggta tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac
     2221 cgcgaatatg ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa
     2281 acaccaaaac aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa
     2341 ggctttgaaa cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca
     2401 cgtaattttt atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa
     2461 ttgctgcgcc actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac
     2521 tttatcgata tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt
     2581 ggtctttcag gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt
     2641 gaccaattga catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa
     2701 gtaatggaaa gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat
     2761 cgcttcttag acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg
     2821 aatgaattcg tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc
     2881 tctttcctag cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca
     2941 cagacgatca aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg
     3001 atcgaaccat taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc
     3061 atgcctgatt caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt
     3121 cttggcgaaa tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt
     3181 gaagaaatcg cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca
     3241 gggatccgct ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc
     3301 cgtgctgtaa cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc
     3361 ttgcaaaaaa tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat
     3421 gacatcactt ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg
     3481 agggggaatc cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa
     3541 ataagatggt tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca
     3601 taaaaagaag ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt
     3661 gctcaaatca tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt
     3721 atctgcacaa ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta
     3781 gcataataca aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga
     3841 aatcgtttct ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc
     3901 taacgatgtg gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt
     3961 tcaccaatga aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc
     4021 caatttcaca aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac
     4081 ttgttttaac aaccgcagta tttagttccg ctccaccata atttttgata tataactgat
     4141 tagtatcaaa ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta
     4201 tgccactaac attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt
     4261 tgtagtcccc aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt
     4321 gaaaccaggt ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat
     4381 ttttaactag atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt
     4441 ttccagcaaa atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac
     4501 tgagataatt catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt
     4561 cccaatagaa gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat
     4621 ccagcaaata ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg
     4681 cttcaccaaa agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt
     4741 caactacgat cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt
     4801 cattcattga accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac
     4861 ctcgactcgt gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta
     4921 agtggagcat tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact
     4981 tcaaaaaagc agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag
     5041 gccagagcat tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca
     5101 gctgcttcat ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt
     5161 caaaggtcag accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat
     5221 cactgtagtg aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt
     5281 caatcgtcgt ttccagatca tttgtccctg ctccataact ttctccatta ctattataag
     5341 gatcctgcca caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg
     5401 catcttttga aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga
     5461 tcgaaatatc tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag
     5521 cccccaaagc ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt
     5581 tcattatggc tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga
     5641 tctctcgtta atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga
     5701 tcggtgtctt ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata
     5761 tcaaaatcct gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt
     5821 gctttcgatt cagatgcacc tttttttaaa gcattagcta acatcccact tgtccctgcg
     5881 ccagcacaaa gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct
     5941 gccacaggta cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct
     6001 gctttttgtg cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag
     6061 atcaggaagt caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc
     6121 gttcctagaa ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg
     6181 ttcatgccca atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac
     6241 aaaaatggaa taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg
     6301 ttgacagcga aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac
     6361 ttagcaaaga gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt
     6421 cctacaaaat tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac
     6481 aattgcagat tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc
     6541 gacggtccat gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt
     6601 cctaagtacc cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca
     6661 aatgagattt ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa
     6721 aaagagaaag gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc
     6781 attttaatgg taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca
     6841 gcgacaaagg aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca
     6901 atcggagaga cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg
     6961 ttgattactt tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg
     7021 gttgctgaaa cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat
     7081 gtggagacag attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg
     7141 aaaatgcttg aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct
     7201 aaataactat tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg
     7261 attccatcca tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct
     7321 tgaatcagat ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg
     7381 aatcccatct ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta
     7441 tgtgcaaggg ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt
     7501 ccttgtctta cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca
     7561 acgatctcaa aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc
     7621 acttattaaa taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt
     7681 gggtaacgct tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca
     7741 tttgtccatg agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg
     7801 gctgttatcg ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga
     7861 tccataataa tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag
     7921 attttgttgt aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct
     7981 ggataaatca gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg
     8041 attcccttca ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca
     8101 ttttctccat cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca
     8161 ttacgcgtgg atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc
     8221 tgatttgctt cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa
     8281 taggtagcat ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta
     8341 tcttccggcg cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca
     8401 cccggaagtt cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta
     8461 tgaagacagg cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac
     8521 aaatattgat tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct
     8581 ttgaattcat gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc
     8641 aaaaaatctc catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac
     8701 ggcgttacgt ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct
     8761 ggatttggtt tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga
     8821 ccatttacac caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca
     8881 cttgccggat cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg
     8941 cctttgccac cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa
     9001 ataaaatctt ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg
     9061 ctctttctaa ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac
     9121 aaataatcat aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac
     9181 cctaaatgtt tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat
     9241 ccttaaatta gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat
     9301 ggtcaataaa aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga
     9361 tatgaccgtt cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca
     9421 tggaggggct caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc
     9481 cactctgcag attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga
     9541 gggagaaacg attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact
     9601 gaacaagaaa attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc
     9661 agatggacca gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt
     9721 cggaccgatc accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc
     9781 aaatggcgtt cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca
     9841 gatcgctcta aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg
     9901 agatgatttt tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca
     9961 gatctcgcgg gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc
    10021 aaccgaaaat accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga
    10081 acaatgtttt tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat
    10141 acaaaagatg gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg
    10201 gagattttga aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca
    10261 gaagaaaaag aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg
    10321 acaaaagggc ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt
    10381 gccacggcta ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc
    10441 tggtttgact gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg
    10501 gatatttttc ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc
    10561 gaagatgctt actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca
    10621 attgatccat tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga
    10681 accatcaaag attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct
    10741 taaacgattc aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa
    10801 aactgccagt gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg
    10861 tatctttgac tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac
    10921 attaaaaata gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact
    10981 tttttgatat acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc
    11041 tccaccaagc tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa
    11101 cgtttccgta tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc
    11161 attaaactta agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac
    11221 aattttgtct ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc
    11281 tgaagaaata atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg
    11341 ggattgattg atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc
    11401 atactccgca aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca
    11461 accaggagaa atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg
    11521 agtgttgatt ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat
    11581 aagctaatta agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt
    11641 gtgtagctac ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac
    11701 tgcattaaag ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg
    11761 tcaaaatttt tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc
    11821 ttctacttca gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga
    11881 tccttctctg taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc
    11941 tttccactca ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt
    12001 tcccttttct accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta
    12061 catctagtaa attttcatca atttctatat cataaagtat ttcactagtg tctacatcca
    12121 caatatatac tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc
    12181 gtcactttca tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag
    12241 ctctaagcct actgtatttt ttactattga gtcattattt accaccataa cttcgtactg
    12301 tatataccat cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt
    12361 ctccggaaca atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc
    12421 atgttcctca cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa
    12481 aataaacact acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc
    12541 caatcacaaa ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac
    12601 tacaactatt catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga
    12661 tacttgtaat agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa
    12721 ttccaataca caacttatcg actatt
//
LOCUS       DAZAJZ010000082        12737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000082 DAZAJZ010000000
VERSION     DAZAJZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.083"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG86D_002245"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG86D_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN3141371.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG86D_002246"
     CDS             complement(323..892)
                     /locus_tag="ACG86D_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN3141372.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG86D_002247"
     CDS             1078..1980
                     /locus_tag="ACG86D_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3141373.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG86D_002248"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG86D_002248"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN3141374.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG86D_002249"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG86D_002249"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN3141375.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG86D_002250"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG86D_002250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN3141376.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG86D_002251"
     CDS             8548..8733
                     /locus_tag="ACG86D_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN3141377.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG86D_002252"
     CDS             complement(8795..9991)
                     /locus_tag="ACG86D_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN3141378.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG86D_002253"
     CDS             complement(10161..11597)
                     /locus_tag="ACG86D_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN3141379.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACG86D_002254"
     CDS             11890..12699
                     /locus_tag="ACG86D_002254"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3141380.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4011 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaat
//
LOCUS       DAZAJZ010000083        12589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000083 DAZAJZ010000000
VERSION     DAZAJZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.084"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..1972
                     /locus_tag="ACG86D_002255"
     CDS             89..1972
                     /locus_tag="ACG86D_002255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN3141381.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2047..2331)
                     /locus_tag="ACG86D_002256"
     CDS             complement(2047..2331)
                     /locus_tag="ACG86D_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3141382.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2321..3055)
                     /locus_tag="ACG86D_002257"
     CDS             complement(2321..3055)
                     /locus_tag="ACG86D_002257"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN3141383.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3184..3807
                     /locus_tag="ACG86D_002258"
     CDS             3184..3807
                     /locus_tag="ACG86D_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN3141384.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3999..5120
                     /locus_tag="ACG86D_002259"
     CDS             3999..5120
                     /locus_tag="ACG86D_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN3141385.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5127..8255
                     /locus_tag="ACG86D_002260"
     CDS             5127..8255
                     /locus_tag="ACG86D_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3141386.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8274..11468
                     /locus_tag="ACG86D_002261"
     CDS             8274..11468
                     /locus_tag="ACG86D_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN3141387.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11614..12321)
                     /locus_tag="ACG86D_002262"
     CDS             complement(11614..12321)
                     /locus_tag="ACG86D_002262"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3141388.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4285 a         2218 c         2652 g         3434 t
ORIGIN      
        1 tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa
       61 aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg
      121 ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca
      181 ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc
      241 tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat
      301 ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt
      361 gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt
      421 tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta
      481 tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg
      541 tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag
      601 aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat
      661 cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt
      721 ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa
      781 catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact
      841 gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct
      901 cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt
      961 gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta
     1021 tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca
     1081 tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg aactttctta
     1141 tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa
     1201 aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt
     1261 acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga
     1321 tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa
     1381 caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc
     1441 caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat
     1501 gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt
     1561 cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt
     1621 gaaagacact gttctgatcg gactaggaac gaatggctca ttctcggaag ctcagtttga
     1681 tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac
     1741 gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa
     1801 tatgacgctg attgattggt acagctacag taatgaccat agtgactggt tttatgatga
     1861 tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt
     1921 gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca
     1981 aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt
     2041 taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt
     2101 ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga
     2161 aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc
     2221 accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata
     2281 aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct
     2341 cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt
     2401 tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc
     2461 caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct
     2521 gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta
     2581 gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga
     2641 ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat
     2701 tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga
     2761 tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt
     2821 ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa
     2881 aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac
     2941 gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga
     3001 taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt
     3061 ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag
     3121 gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag
     3181 actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga
     3241 aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct
     3301 ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc
     3361 agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga
     3421 tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta
     3481 aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct
     3541 acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca
     3601 gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg
     3661 cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg
     3721 gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat
     3781 ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa
     3841 tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg
     3901 ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt
     3961 gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg
     4021 attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac
     4081 tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag
     4141 acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg
     4201 tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca
     4261 gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac
     4321 agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt
     4381 actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc
     4441 aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag
     4501 tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg
     4561 aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac
     4621 taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc
     4681 cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga
     4741 caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa
     4801 cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt
     4861 acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc
     4921 aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc
     4981 gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg
     5041 aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag
     5101 aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg
     5161 gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga
     5221 tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt
     5281 ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac
     5341 cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg
     5401 aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag
     5461 ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac
     5521 gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc
     5581 aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata
     5641 aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat
     5701 taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc
     5761 ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg
     5821 ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag
     5881 aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg
     5941 aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag
     6001 ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc
     6061 aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt
     6121 cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt
     6181 ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa
     6241 aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag
     6301 acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc
     6361 aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg
     6421 aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc
     6481 aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac
     6541 tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac
     6601 aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt
     6661 tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa
     6721 aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg
     6781 agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc
     6841 ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg
     6901 aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa
     6961 aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg
     7021 ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat
     7081 tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag
     7141 aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga
     7201 tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag
     7261 caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg
     7321 aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt
     7381 tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag
     7441 cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag
     7501 ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag
     7561 agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat
     7621 acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt
     7681 atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat
     7741 tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc
     7801 aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat
     7861 ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct
     7921 atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta
     7981 ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg
     8041 tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg
     8101 aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta
     8161 gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta
     8221 gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat
     8281 ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt
     8341 tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat
     8401 tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt
     8461 gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa
     8521 atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg
     8581 agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg
     8641 tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag
     8701 agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat
     8761 atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg
     8821 ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa
     8881 tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac
     8941 aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc
     9001 tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc
     9061 aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag
     9121 aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg
     9181 gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt
     9241 ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac
     9301 cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc
     9361 ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag
     9421 caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg cgaagtcatc tattcgactg
     9481 acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag
     9541 agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg
     9601 aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct
     9661 tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc
     9721 ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg
     9781 atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg
     9841 atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac
     9901 aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc
     9961 atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt
    10021 ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac
    10081 agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt
    10141 tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga
    10201 aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg
    10261 ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg
    10321 ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg
    10381 ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt
    10441 atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca
    10501 gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg
    10561 ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag
    10621 gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga
    10681 ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa
    10741 cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact
    10801 tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga
    10861 tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta
    10921 ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg
    10981 ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct
    11041 tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac
    11101 cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga
    11161 tttccttaaa ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt
    11221 atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg
    11281 gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca
    11341 tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag
    11401 aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg
    11461 aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg
    11521 attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc
    11581 ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc
    11641 taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg
    11701 ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt
    11761 gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc
    11821 tagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt
    11881 gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt
    11941 cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt
    12001 tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa
    12061 gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg
    12121 atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc
    12181 actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac
    12241 gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc
    12301 aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca
    12361 aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc
    12421 ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa
    12481 aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat
    12541 tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttattttt
//
LOCUS       DAZAJZ010000084        12169 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000084 DAZAJZ010000000
VERSION     DAZAJZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.085"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..788
                     /locus_tag="ACG86D_002263"
     CDS             162..788
                     /locus_tag="ACG86D_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3141389.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            811..1515
                     /locus_tag="ACG86D_002264"
     CDS             811..1515
                     /locus_tag="ACG86D_002264"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3141390.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            1707..2540
                     /locus_tag="ACG86D_002265"
     CDS             1707..2540
                     /locus_tag="ACG86D_002265"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN3141391.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            2737..4665
                     /locus_tag="ACG86D_002266"
     CDS             2737..4665
                     /locus_tag="ACG86D_002266"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3141392.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            4803..5258
                     /locus_tag="ACG86D_002267"
     CDS             4803..5258
                     /locus_tag="ACG86D_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN3141393.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            5338..5466
                     /locus_tag="ACG86D_002268"
     CDS             5338..5466
                     /locus_tag="ACG86D_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141394.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            5728..5997
                     /locus_tag="ACG86D_002269"
     CDS             5728..5997
                     /locus_tag="ACG86D_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141395.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            6397..6651
                     /gene="rpsT"
                     /locus_tag="ACG86D_002270"
     CDS             6397..6651
                     /gene="rpsT"
                     /locus_tag="ACG86D_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN3141396.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(6740..7786)
                     /gene="holA"
                     /locus_tag="ACG86D_002271"
     CDS             complement(6740..7786)
                     /gene="holA"
                     /locus_tag="ACG86D_002271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN3141397.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(7824..10097)
                     /locus_tag="ACG86D_002272"
     CDS             complement(7824..10097)
                     /locus_tag="ACG86D_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN3141398.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(10090..10629)
                     /locus_tag="ACG86D_002273"
     CDS             complement(10090..10629)
                     /locus_tag="ACG86D_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN3141399.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(10765..10983)
                     /locus_tag="ACG86D_002274"
     CDS             complement(10765..10983)
                     /locus_tag="ACG86D_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN3141400.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(10980..11225)
                     /locus_tag="ACG86D_002275"
     CDS             complement(10980..11225)
                     /locus_tag="ACG86D_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3141401.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(11341..12069)
                     /locus_tag="ACG86D_002276"
     CDS             complement(11341..12069)
                     /locus_tag="ACG86D_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN3141402.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         3732 a         2319 c         2192 g         3926 t
ORIGIN      
        1 atgaaaactt actttccttc tctttagaat atccaaatac ttttcatata gtctgataat
       61 tcagataagt aaagagaaat aagtacctgt ttttgtttcc tagcaatgaa tgcgttatac
      121 taatggatga aagaagtttt ggctatcaag gaggaatgca catgcatatc gaaagattaa
      181 aaacaggaat gattgaagaa aactgttatt tggtctacaa cgaagaagca cttttgatca
      241 ttgaccctgg agaagatgcg gaacgaatca aacaagaaat caaaaaaaca aaccaacagc
      301 cggtagcgat tttgcttacc catactcact acgatcatat cggtgcagtg gaacctttac
      361 gcgcgtatta tcaaatccca gtttatgtca gcccactaga gcaaaaatgg ctaggtgatc
      421 cgattttgaa tctttctgga ctcggtagac atgatgatat tgctgatgtt attgtctcgc
      481 ctgcagaata tgaatttgaa atgaaaactt atactttagg aaacatgtct tttcgcgtcg
      541 tccctacccc agggcattct ataggaagcg tcagctttat ttttgatgat tttgtcgttt
      601 ctggcgacgc cctgtttaga ggaagtatcg gtagaaccga tctgtataca gggaatttag
      661 aacaattact tcatagtatt cggacccagc tgtttgtatt acctgatgaa tttgcagtct
      721 atccaggaca tggcgatgca acgacgattg aacaggaaaa aaggacaaat ccttttttca
      781 atcgttaaaa cgacttagga ggagaataaa atgacagaaa ctacacttta tatcgtacga
      841 cacggaaaaa cgatgttcaa tacaattgaa cgagtacaag gctggtgtga tacacccctc
      901 acaaagcaag gacaagaagg cattcattat ttaggtcgcg ggttaaaaaa tgtcgacttt
      961 tcatttgctt attctagtga cagtggtcgt gcaatcgaaa ctgcccgtat tattctagga
     1021 gaacattcta agggaaaaga aattccttat tttattgaaa aaaggattag agaatggtgt
     1081 ttcgggtctc ttgaaggtgg atatgacatg gaaatgtggg gtgtgatccc tcgtgtattg
     1141 aattttaaaa catatgatga aatgtttacc acagaagttt cttttgaaca aattgctaat
     1201 gctgtataca acttagatac cgctgggtgg gcggaaactt atgaaacatt gcgagaccgt
     1261 gtatggacag gatttgaaga tatcgcccat cactgcgaaa aaaatggtgg tggaaatgtt
     1321 ttagtcgtat ctcatggttt gacgatcgcc tttttgctta atctgatcga tcctaaacag
     1381 cctgttcgtg ctgggctttt aaatggcagc gtaacaaagg tcacttatgc aaacggtcga
     1441 ttttctatcc acggcatcaa tgatacgtct tatatcgata acgggaaaac acatagtcgc
     1501 gactcgtttc tataatgggg atattttaat ttttaccaga gaatcataaa aggcaaagtc
     1561 tgaacgaggg ctttgtcttc ttttttctct tcgtgtactt atctgcttta tttctcaagt
     1621 ttttcttaat attaaaaaac agattgggtt tctcaaaaaa taacggtata ctataagatg
     1681 tatcttaatt gggaaggaag gaatttatgt tttttgcaaa tattatcaaa gcagtgattc
     1741 tcggtattat tgaaggaata acggaatggc tgcccatcag tagtactggt catttgatcc
     1801 tagcagatga attcatcaaa ctggatgcca gttcacaatt tatgtcgatg ttcaatgtcg
     1861 ttatccaatt aggggcgatc ttggctgtcg tcgttcttta ttttcacaag ttaaacccat
     1921 tttctccaag taaatcaacg gtagaaaaaa aagatacgtg gacactttgg tttaaagtga
     1981 tcgttgcttg cataccagcc atcatcattg gtttgccttt ggatgattgg ttagaagcaa
     2041 atttccacaa gttcttacca gttgccattg ttttgatcgt ttacggtatt gcctttatta
     2101 ttgtcgaaaa acgcaacaaa aacaaaacac ctcgttggac ttctctgaat gactttacgt
     2161 accaagcagc tcttcttgtt gggttattcc aagtcttggc acttgttccc ggaacatccc
     2221 gttctggcgc aacgatccta ggatctatct tgattggtgc ttcgcgtttt gtcgctacag
     2281 agttttcatt tttcttaggg attcctgtca tgtttggcgc aagcggcttg aagatcgtca
     2341 aatatttagc tgaagggaat tcattcaaaa tggaagaaac tgttgttctt cttgtaggaa
     2401 cattcgtatc ttttgtggtt tccgtcttag ctatcaaatt cttactaagc tatttgaaga
     2461 aaaacgactt taccgctttt ggctggtatc gtattatcct tggtatcatt ttgattggtt
     2521 attggtttat tgcaatgtag aaaaaaccag aacacttctc ttatttgaga aatgttctgg
     2581 ttttttattt tctttaataa taatcattat aaataataaa aacaacatca agcttaatca
     2641 ccaaatgata accattatcg ttctcattta gctgttatta ttatcaatta catttttatt
     2701 atgctatcct tgttttgaca aggaggtcta ataacgatga ctaatcagaa aaaagctttt
     2761 gcagcaacaa tcggctgcct tttatttatg atcttcggtt ttatcttcga ccgattctct
     2821 cttaccatca ccccactttt ttatatcagt gccattatca tcggtggata taaacaaaca
     2881 tcggaaggct tgaaagaatt gtggaatgat cggacactga atgtggattt attgatggct
     2941 ttagctgcta ttggtgcctg tttgatcggt aattactttg aaggagcaat gttgacattc
     3001 atcttttgtt taagcggtgc attagaggaa tacacaacta ataaaagtca aaaagaaatt
     3061 actgctttga tgaatcttca accacaaaaa gcccaattat taaaagaaaa tggacaaatg
     3121 caagaagtag atgtttctca actaaaggtg gataacttgg tatttgtcgc taaaggagca
     3181 gctgttccga tagatggctt tttagaaagc acacaggcaa cgatcgatga gtctgcatta
     3241 agcggtgaat ctgtgcctgt agaaaaatat ttaggcgatg agatgtatgc aggaacgctg
     3301 aatcaaggaa atcccatcac gattcgagta acaaaaacga gttccgaaac tgtctttgct
     3361 aagattatcc aattagtaga agaagcgcag aatacgccaa ctcaaacagc ttcatttatc
     3421 gaacgaatcg aaaataatta tgtaaaattg attttgcttg ctgttcctct aatgatcctt
     3481 ctcccccact ttttcctagg ttggagttgg gatgaaagct tttatcgagg aatggtatta
     3541 ttagttgtgg cttctccttg tgcgcttgtc gcatctgcta caccagcttc tctagctgct
     3601 ctttcaaatt ctgcaaaaaa tgggatttta atcaaaggcg gtatccattt agaaaagctt
     3661 tctgaattaa aagcgattgc ctttgataaa acaggaacgc tcactaaagg aaaaccagtt
     3721 gtaactgaca gctttttctt tgatgaaaag gatttggcac aacaacttct tgttgctatg
     3781 gaacaaaaaa cgacgcatcc acttgctcat gcgattttgc aatatttcga ttgcacaata
     3841 cctgaagaaa tccaatactt accaatagaa gaaattactg gttttggatt acaaactacc
     3901 tatttaggtg cgcaatggaa agttggcaag cacgcatatg atcctgagac gatgataatc
     3961 agcaaagaga ttgcggaaat gatcgaacgt ttggaaaatc aaggcaaaac cgtgatttat
     4021 ctttctaaag atcagcagtt aattgctgtt ttaggtctgc tagatattcc aaaagcaaat
     4081 acccaacaag taatttccta ctttaaatcc caaaacatcc atacaagtat gataaccggc
     4141 gaccattctg gcacagcaaa ggccattgct gaacaagtag ggattgaaca ttactatgca
     4201 agctgtactc cggaagaaaa aacacaacta gtcaaaaaag agaatgaact ttaccaagta
     4261 aatgcgatgg ttggtgatgg agtcaatgat gcgcctgctt tagcagccgc ttctttaggt
     4321 gtcgctatgg ggcaagggac agatgccgct atggatatcg cagacatcgt cttaatgaaa
     4381 agcgacttaa ataaatttgt ctacagtcat cggctatctt taaaaatgcg gcggatcatc
     4441 aaacaaaata ttattttttc gattcttgtt attctcttgt tgattgcttc taacttcctg
     4501 caatttttaa atctacctct tggagttgta ggacacgaag gaagcacaat attggttatt
     4561 ctgaatggct tgcgtttact aagaccttta cctgatcttt ctgattcttc aaaaaaatgt
     4621 aagtcttgtc cactatacaa agcgcggctg tcttctgtca aataagcaaa gtgttttttt
     4681 atgattttgt ttattttatg aaaaaaacag cttatgaata ggtcttgaag gatgaattcc
     4741 tacttttgta actttctaaa aacatgttat gattgtttcg aagaaagtga ggaatatatt
     4801 tcatggaaag ttggcttttt ttaggaatta ttttacttgt agcaatttta gctaaaaatc
     4861 aaagtctgat gatagcggca gcttttgttt tgattatgaa gctcatacca gcctcccgtt
     4921 cctttttccc ttggcttcaa ggcaaaggaa tcaatattgg ggtgacgatc atcacagcgg
     4981 ctattctaat ccctatcgca acaggagaaa tcggtctacg tgatctatta gaagccttta
     5041 aatcccctat tggctgggta gctgtttttt gcggtgggct agtggctgtc ttatcttcca
     5101 agggtgtagg attgattgct tctgatccac aaatcactgt tgcacttgtt ttcggcacga
     5161 ttttaggtgt tgtcttctta agaggaatcg ccgccggacc ggtcattgct tctggtatca
     5221 cttactgtat cttacaagta tttgctttag gaaaatagaa caaattttgt ctattttagg
     5281 agattcttaa cacccccaat ttactgatcc gtgatatact gacaacgagg tgatacaatg
     5341 ttcgaaaaat taaagtattt cataggaaca atcggctggt ttcttctagc ggtacttttg
     5401 attctgggct tagcttatct attatttcct gatttgatac aaaatgtgtt tgatactgct
     5461 ttttgattga cctattaaaa cgatataggg gatgctcctg cgatgcaggg gcttttttta
     5521 atcaatgttt tgtaataatt taggccgtgc caatgagact tcctatagaa ttcgatgctc
     5581 ttttttcttt ttggctcttc tgctcttttt tgttatacta gattacagtg gacaaaagaa
     5641 agaatgatcg catccacttc aacgaatatc aaaatggaag aattagtgcc tgttttcagg
     5701 ttactttgaa aagaaaggaa gacgcttgtg gaaacttttg aaggtctttt aattgatacg
     5761 tacgttttac aagaacagcc aatgatgctg actgggatca acttgcagct tgaggaccgt
     5821 gtgatcaatg gcctgcttaa agatcgctat ttggcaaaat atatcttacg tttgccccta
     5881 aataaatatg tgctggaagt aagtggcaaa tggaaccgtc ggaaccagtt ggtcataaaa
     5941 aaaatgttga taaaaaatat cgatgaatac attaaaataa tcggtacacc agaatagcct
     6001 ttactgatca aaggatactg gttataaaat aactgcagtt tcttaataga aaaacaaaga
     6061 acaacaggga atcgtatccc ttttcaaaaa aaactttgca aacggttctc tttgttatat
     6121 aataacttag taccaaacat tcccattcaa actttgttta aatagataaa taaaaatcta
     6181 gtttggtttg acgctattgc ctcccacaat agcgtctttt tgattttttt cagcttgtac
     6241 tatccgtttt tactgaaaaa aaatcctaat aaaagactgt taaggaaaaa aacttgtcat
     6301 gttcatattg caaagcacgc tgcctttagg tataataatg aatgttgaat tatgcggtaa
     6361 cacgcgtaag aacttaagtt atcggaggtg aaactaatgc caaacattga atctgcaatc
     6421 aaacgtgttc gtacaagcga aaacgctaat gtgaaaaatt catctcaaac aagcgctatg
     6481 cgtactgcaa tcaaaaaatt tgaagatgct gtagcttcag gtgctgacaa cgtagatgcg
     6541 ttatataaag aagctgttaa agctattgat atggctgaat caaaaggtct aatccacaaa
     6601 aacaaagcta accgcgacaa atctcgttta agcaaaaaaa tcgctaaata aaagaatcgg
     6661 cttcggccga tttttttatt tgtcaaaaaa aggacaaaaa caaacgacct tcccgcatgc
     6721 tttgccctcg tctttcttct tatcgatttc ttaacaactg ccctgtgcgc aaaacgaata
     6781 gttgaaaact taattctttt tcgatttgtc ccgttttgat ttgataatcc atctcaacta
     6841 attgatcaaa caactgtacc agcaaatgtt catcgaattt ttttacttcc tgcatggcaa
     6901 gttttacacg atatggatgg attttcaatg tttcagcaat atttgcctgc tgataaccaa
     6961 tctttacaag aaattttgtc tgtaaaaata aacgtaattg agaaagcaaa atcgcattaa
     7021 ttttgattgt ttcttcccct tgcataacta aatcttcgta taaacgcaaa gcttgttccg
     7081 tcttcccttt taaaatatat tgggtcatgt caaaaatatt atgttctaac gttttaggaa
     7141 ctaattcttc tacttctctt accgtgattt gctgttgatt ttgtccataa aggaccaatt
     7201 tctgtaactc tctcatcatt ttggataaat ctaagtcggt caacctcaag aataaatcaa
     7261 ttgcacttcg ttccattttg attgaggaat tctctaaggt attcatcaga tattgacgta
     7321 cttctttttc attcaactgt tggacattga tgataattgc tgtttttttc aattgtttcg
     7381 ttactttttt ccgttcatcc aacttttcat atggcgcaaa gaatactaat acagtcgatt
     7441 ctaatggttc tttcaaataa gccgtcaata aatcagtatt ttgttcgatt ccattgttta
     7501 ctttttcacc tgttaaaaaa taaggattct ctacgaatac taaccgttga tctccgaaaa
     7561 acggcaacgt ctctgcttca gctactacct catctaaact tgcattttcc atgtcaaaac
     7621 ttaaaaaatt taagtcatct tcgccctcta attggatttt tttgacaatt tctgtccgaa
     7681 cttgatcttg gagaaacttt tctgttccta aaatcaagta acaagatgca agtttttctt
     7741 tacgtatttt ttgtaactct gcttgtatac tcaaattact caccactttc aaaaattatc
     7801 ctaccatgag cagatgagaa ttactagtct atcattgttt ccactagccc atgaggatga
     7861 aaaactggat gccagcgata ggtgatcatt ccttgttgat ccgttcgcca tgtttttatc
     7921 tgattttctt ctagtgtctc caatacttcg ctgtgcgggt gaccataacg attatttacg
     7981 ccgcaggata taatcccatg ttttgcttca atatgggaaa gaaaatctgt gttacttgac
     8041 gtacggctac cgtgatgacc taatttgact acatcggctt tcagattagg ataaatcaaa
     8101 agcaattgtt tttccattgc ttgattcaaa tctcctaaaa ataaaaagcg cctattgtta
     8161 tattccatcc atagaccaat cgaatcttca tttcttccct ctcccttttc aaaaggagtc
     8221 aatacataaa gatcataata accgctgatc ctgtccccat gtttgatttc aatgatctca
     8281 gtaccatttt tttgagcttc agataaatgc ctttgtaatg gctcatttcg tccagcgccc
     8341 caaccgatat aaattttttt gatagtaaaa tgctgagata acgtgggtaa ttctcccaca
     8401 tgatccgtat cgtcgtgtgt caagatgagt ttatcgatta cacttatccc ctgacctttt
     8461 agatatggaa cgatatttct ttcagcagga ggacgtatca taccttgcgc ccacttttct
     8521 ctttcaaagt gtaactgacc tccagtatcg atcaaaatcg tttcttttcg aaaaggactt
     8581 tgcaaaaaga tactgtctcc ttgaccgaca tcgataaaag cgatcgttga agaaaagcgt
     8641 aaaaatggca aagtcaaaaa aataaaagaa aaaagaccaa cataaaacaa gtgtttcaca
     8701 ggttgttctt tcaacctatc catacaaatc agtaatccga aaatatacat cccagatagc
     8761 acaaggggca aacgtcctat aatgagagac gaccctgaag aaaaatcgat tattcgctca
     8821 aataagatta atccctcttc tatcagcaaa agtaaagttt ccggcagcca gcgattgaag
     8881 agtgtgagaa ataacacacc aggaagaaaa agaaacttga ataccggtgc aatcagtaat
     8941 gtaaaaagac tacctaaaat aggccactca taaaaatgcc atacaatcaa cggcatcgtg
     9001 atcatagtaa acaggacgtg agaggccgtt tttttcaacc aatctccatc aaaagaaatc
     9061 aggataaaca aaaaactcat gaaaaaacta agctgtccac ccgcatgaaa aagaaatcga
     9121 ggatcaaata gtaaaagaag ccaaaagaca actgcaaata agtccatggt ggaataatgc
     9181 ttattcagca atttgactag caatcgcaat aaaaacagca aactagcacg caaaacagaa
     9241 atagaacttc ctgcaataga aacataacag atagttagta gactcaacgg aataatactg
     9301 acgtgtagtg gccatccgat acgtcgaaac aaataataca gccaccctaa ataaaattgg
     9361 atgtgtagac ccgatagact gaacagatgc agcaaaccac tctccctaaa aatttcttct
     9421 ggttctctga atcctgcatc tttatagccc cataataaag aattaatata tacacttgtt
     9481 ttttgcggaa attcagattg gacaaaggag ataaaagatc ctctttttct ctgaagccag
     9541 tttcgccagt gattttgcat ggtgatctgt tcaatctttt gaatttggaa ccgtccagaa
     9601 attctatggt tcctgaagta agcttcttgg tcaaaattat aaagattccg cttttttgct
     9661 ggagagatga actcgccttc agcagtcaat tctattccct ttcctcggaa gttaagccat
     9721 gttttcaatt ctgcttctgt gtttacttga taagaaagct ggagcttttg tttattctct
     9781 gataaccttc cttccattgt caaaaaatta ccatttgcac gaatcgtatc tgtaaataca
     9841 gtaatttgcg cagatgttgt tttttcctct aaaaatgaat actcttgtag cctataagaa
     9901 atggataccg ttatgctaca caaaatacac aagtacgcga taaaacgtcc ttcttttagc
     9961 gaaaaccata aaaaaattcc ggcaagaagc aaacgataac cccaattgtc agtgtaatac
    10021 aacaaacaga taatcatcga cgtaattgcc ggaaaaatca accgatttcg aacatgcctt
    10081 tctaaaacgc tagacatgtt ttcctgttaa ttcattttga agttttgaaa aatgctgtga
    10141 gtctaaagca acttggtgta cagatacacc taattgttcg atcaacgctt gggcataagg
    10201 atcatttcga tagtcatgga gataattgat ccctttcaca ccagcttgca acaaagcctt
    10261 tgtacaggcc aaacatggaa aatgtgtgac atagatttct gaaccttccg tcgggatacc
    10321 aagttttgca cactgtagga gtgcattcat ctctgcatgg atcgttcgca agcagtgtcc
    10381 atccacgacg taacaccctt catctatgca gtggacatct ccactgactg ctccattata
    10441 gccgccagca atgatccttt tttctcgaac gagtgtcgct cctacttcta aacgtgtaca
    10501 cgtactacgt aaagataaca gtactgcctg agccataaag tactggtccc aaggaatgcg
    10561 ttcattatgt gacttatcag taactttttc tttattcgta tcgcatgctt ttgaagattt
    10621 ttctaacatg ttttatattc ctccgttttt tcaagttgat taaaaagtac tcattgctta
    10681 aacaaaatat tctttaaata taacgaatca tcctgtctga caaaagatcc tacccttcac
    10741 tgccgaaatg aagtccgtca tccattagcc cattataaaa attcttcgaa tccatttgtt
    10801 caagaaattc cgtataaaaa tcgatacttc acaatcatgg cgttttgcta cttctgtgag
    10861 aacagagata tattctttga tcaacttgtt attccgatat ttttgttttg tctcatcgac
    10921 tgctggtggc gtgataaaga aagcatgatc agaactatag tcttttttca gctgaactat
    10981 catccactgc agattttttg gtacgtttct ttagaaagtt gtttatgctc tgctagatca
    11041 ttcgtcccaa tgagaataaa tactttatct gctgtatagc taagtacatc tttggcaaag
    11101 cgttctcgta gagcttgact gttattgccg ctgacagccg tattcataat atgaatccca
    11161 gatattttat tttggagata gacattcagc attggttttt ttaatgtttc tttgcgaaca
    11221 atcaagctat ttcctatcca aaaatactac ctcctcgtcc tgccaagctc ttagaattgg
    11281 attattctta gtatccatta gcgtccattt attgctcgtg ccagtcgatt ttgaatcatt
    11341 ctatttaatt gtaacgaagt tttttagttt ttcaactgtc ttttcaccga taccagaaat
    11401 ctcctgcagg tcttccactt tctggaagga tccgttttct tctctaaatc gaatgatttc
    11461 ttgcgctttg actgatccga tcccaggaat ctgctgaagt tctgatggag tggctgtatt
    11521 gatatttatt ttgggaggct cggaatctgc tgtaggctgt tttccttccg aggatgccaa
    11581 ctgatcaaga tgttctggca gctcttcacc tacagcaggg atatatagga cttgctgatc
    11641 ttctatcttc gcagaaaagt tgattcgatc gctatccgct tcttctaata atccacctgc
    11701 acgctttagt acatcataga cccgttcttc tgaagaaaag gaatagatac caggctcctt
    11761 cacacttcct ttgatatcgg tgtacatgac ctttggacga gcatcctctt ctacatcgtt
    11821 tccttcagct gatggatctt ctccactgga actaaacgaa gaaagtattt cttttgcagc
    11881 agtctcttct gtttctctat aaaatagcaa atttgataat aaaaaaccac ttgcgataca
    11941 aaagataaga actatcatag acatccaaat gatcaatttg ggtgaagcta gtattttctt
    12001 gctgattttt tctatcattg ttcttttttc aaagaatgac cagtaaaaat cattcattac
    12061 tcctttcatt taaaattgaa gaaacagtga gctctggcaa aatcgtccct tcaacatcta
    12121 attacgtcaa ttttttcttt tgtttttatt tttatcgtga agatttttg
//
LOCUS       DAZAJZ010000085        12136 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000085 DAZAJZ010000000
VERSION     DAZAJZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.086"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACG86D_002277"
     CDS             <1..610
                     /locus_tag="ACG86D_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3141403.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACG86D_002278"
     CDS             723..1001
                     /locus_tag="ACG86D_002278"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3141404.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACG86D_002279"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACG86D_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN3141405.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACG86D_002280"
     CDS             1458..2213
                     /locus_tag="ACG86D_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3141406.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACG86D_002281"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACG86D_002281"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN3141407.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACG86D_002282"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACG86D_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN3141408.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACG86D_002283"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACG86D_002283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN3141409.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACG86D_002284"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACG86D_002284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN3141410.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACG86D_002285"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACG86D_002285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN3141411.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACG86D_002286"
     CDS             6950..7492
                     /locus_tag="ACG86D_002286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN3141412.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACG86D_002287"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACG86D_002287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN3141413.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACG86D_002288"
     CDS             9085..9987
                     /locus_tag="ACG86D_002288"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN3141414.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACG86D_002289"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACG86D_002289"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN3141415.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACG86D_002290"
     CDS             11430..11852
                     /locus_tag="ACG86D_002290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN3141416.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAZAJZ010000086        12131 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000086 DAZAJZ010000000
VERSION     DAZAJZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.087"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..578)
                     /gene="frr"
                     /locus_tag="ACG86D_002291"
     CDS             complement(21..578)
                     /gene="frr"
                     /locus_tag="ACG86D_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN3141417.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(581..1303)
                     /gene="pyrH"
                     /locus_tag="ACG86D_002292"
     CDS             complement(581..1303)
                     /gene="pyrH"
                     /locus_tag="ACG86D_002292"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN3141418.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1439..2320)
                     /gene="tsf"
                     /locus_tag="ACG86D_002293"
     CDS             complement(1439..2320)
                     /gene="tsf"
                     /locus_tag="ACG86D_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN3141419.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="ACG86D_002294"
     CDS             complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="ACG86D_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN3141420.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3560..4039
                     /locus_tag="ACG86D_002295"
     CDS             3560..4039
                     /locus_tag="ACG86D_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3141421.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4256..5347
                     /gene="phnW"
                     /locus_tag="ACG86D_002296"
                     /pseudo
     CDS             4256..5347
                     /gene="phnW"
                     /locus_tag="ACG86D_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5340..6125
                     /gene="phnX"
                     /locus_tag="ACG86D_002297"
     CDS             5340..6125
                     /gene="phnX"
                     /locus_tag="ACG86D_002297"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN3141422.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6471..8162)
                     /gene="argS"
                     /locus_tag="ACG86D_002298"
     CDS             complement(6471..8162)
                     /gene="argS"
                     /locus_tag="ACG86D_002298"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN3141423.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8584..9531)
                     /gene="arcC"
                     /locus_tag="ACG86D_002299"
     CDS             complement(8584..9531)
                     /gene="arcC"
                     /locus_tag="ACG86D_002299"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN3141424.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9646..10665)
                     /gene="argF"
                     /locus_tag="ACG86D_002300"
     CDS             complement(9646..10665)
                     /gene="argF"
                     /locus_tag="ACG86D_002300"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN3141425.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10756..11985)
                     /gene="arcA"
                     /locus_tag="ACG86D_002301"
     CDS             complement(10756..11985)
                     /gene="arcA"
                     /locus_tag="ACG86D_002301"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN3141426.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3318 a         2529 c         2181 g         4103 t
ORIGIN      
        1 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
       61 ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      121 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      181 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      241 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      301 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      361 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      421 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      481 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      541 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      601 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      661 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      721 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      781 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      841 cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      901 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
      961 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1021 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1081 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1141 cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1201 tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct aaagcttctc
     1261 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1321 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1381 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1441 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1501 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1561 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1621 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1681 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1741 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1801 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1861 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1921 ctccgataac ttgagttgct tcgatcaagt catattcaat tgtaccttta tctgttttga
     1981 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2041 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2101 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2161 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2221 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2281 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2341 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2401 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2461 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2521 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2581 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2641 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2701 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2761 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2821 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2881 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     2941 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3001 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3061 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3121 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3181 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3241 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3301 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3361 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3421 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3481 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3541 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3601 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3661 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3721 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3781 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3841 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3901 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     3961 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4021 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4081 tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac atcatttttt
     4141 attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4201 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4261 taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4321 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4381 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcgg
     4441 ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4501 ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4561 ctataccgtt tatgaggtat cgtaacatca aatcctccaa gctcatgtga ttacacagct
     4621 gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4681 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc ttttgtttat
     4741 agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac tagggattga
     4801 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4861 tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt cacttgatct
     4921 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     4981 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5041 aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa tgacagagat
     5101 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5161 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa acggatttgt
     5221 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5281 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5341 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5401 ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa
     5461 tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5521 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5581 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5641 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5701 cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaagggt
     5761 attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga ccgtttcctt
     5821 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5881 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     5941 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6001 ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct ggcgctgata
     6061 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag
     6121 aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa gtaataagaa
     6181 cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6241 aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6301 gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6361 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6421 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6481 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6541 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6601 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6661 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6721 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6781 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6841 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6901 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     6961 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7021 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7081 ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7141 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7201 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7261 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7321 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7381 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7441 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctcttcatcg
     7501 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7561 gctacttcat ggaattggac atataaacga agcaattcat tgattggttc tgctttaaca
     7621 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7681 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7741 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7801 gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc ataatgttct
     7861 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7921 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     7981 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8041 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc gacttgttcc
     8101 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8161 atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8221 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8281 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8341 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8401 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8461 tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc agatggccta
     8521 aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac
     8581 aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc
     8641 taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcctc
     8701 tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt gtttcatttc
     8761 tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga catacacatt
     8821 gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga
     8881 agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac
     8941 aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat
     9001 gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg
     9061 tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc
     9121 ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc
     9181 cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac
     9241 ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg caacacatgt
     9301 atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt
     9361 accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt
     9421 cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt
     9481 agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca tttcattcgt
     9541 cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt
     9601 ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat
     9661 aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc
     9721 ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt
     9781 gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg
     9841 caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt
     9901 gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga
     9961 tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt
    10021 aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc
    10081 ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt
    10141 gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc
    10201 ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg
    10261 ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac
    10321 cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta gtactttagc
    10381 tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg
    10441 atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt
    10501 ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt
    10561 caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc
    10621 tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct
    10681 ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagaccg
    10741 ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg
    10801 accaccacgg cctcgtgaca attcacttga attgatttct aacactttga tgccatagct
    10861 acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat
    10921 tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt
    10981 accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa
    11041 gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat
    11101 cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt
    11161 aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg ctaacacttt
    11221 ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt
    11281 agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc
    11341 ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct
    11401 tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt
    11461 catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa
    11521 gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat cgtatagact
    11581 gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat ctaccatttc
    11641 ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat
    11701 atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc
    11761 ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtctttgc
    11821 aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg
    11881 atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca gtaaaactgt
    11941 ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct
    12001 tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc
    12061 attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa acgtttttct
    12121 atttttaagc g
//
LOCUS       DAZAJZ010000087        11804 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000087 DAZAJZ010000000
VERSION     DAZAJZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.088"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1830)
                     /gene="uidA"
                     /locus_tag="ACG86D_002302"
     CDS             complement(22..1830)
                     /gene="uidA"
                     /locus_tag="ACG86D_002302"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN3141427.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1853..3619)
                     /locus_tag="ACG86D_002303"
     CDS             complement(1853..3619)
                     /locus_tag="ACG86D_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFN3141428.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3632..4426)
                     /locus_tag="ACG86D_002304"
     CDS             complement(3632..4426)
                     /locus_tag="ACG86D_002304"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3141429.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4436..5812)
                     /locus_tag="ACG86D_002305"
     CDS             complement(4436..5812)
                     /locus_tag="ACG86D_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3141430.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(5932..6579)
                     /locus_tag="ACG86D_002306"
     CDS             complement(5932..6579)
                     /locus_tag="ACG86D_002306"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFN3141431.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(6663..8090)
                     /gene="uxaC"
                     /locus_tag="ACG86D_002307"
     CDS             complement(6663..8090)
                     /gene="uxaC"
                     /locus_tag="ACG86D_002307"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN3141432.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8164..9243)
                     /gene="uxuA"
                     /locus_tag="ACG86D_002308"
     CDS             complement(8164..9243)
                     /gene="uxuA"
                     /locus_tag="ACG86D_002308"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3141433.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9246..10877)
                     /locus_tag="ACG86D_002309"
     CDS             complement(9246..10877)
                     /locus_tag="ACG86D_002309"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN3141434.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11187..>11804
                     /locus_tag="ACG86D_002310"
     CDS             11187..>11804
                     /locus_tag="ACG86D_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3141435.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNE"
BASE COUNT         3793 a         2366 c         1536 g         4109 t
ORIGIN      
        1 aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg atgaccatcg
       61 tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga ttcctttctt
      121 atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat tccacacttg
      181 ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt aataagccac
      241 ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta ctgtatctgc
      301 tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc catctagctc
      361 ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc aaccataata
      421 acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg gcgttgattt
      481 tagataagta acgattgtta caggtctttt ctgcggatca agttctttag ttaattcaac
      541 taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat ttgcaataga
      601 ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga tcaccgtttt
      661 atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat tatcatctaa
      721 taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa taactacaat
      781 accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg atgttctaaa
      841 agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag cctcattcaa
      901 accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat aaaaaggttt
      961 attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa attcttctgt
     1021 ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat ataaataagc
     1081 gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct ttccttcagc
     1141 ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc cggtccattc
     1201 tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat taatagtaat
     1261 atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa tacctgcata
     1321 attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt cctgtactgt
     1381 ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta cagcaactgt
     1441 aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg gtgtaaaccc
     1501 acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat gtgttgcaga
     1561 tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca cttctttttc
     1621 ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat cattataaga
     1681 agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag caagttcttc
     1741 ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt caattacttg
     1801 cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa tattaaataa
     1861 atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat tcagaaagac
     1921 ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa gtattaatat
     1981 aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat acaaaaattg
     2041 ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct ggccagacaa
     2101 ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca tattttttag
     2161 ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta attttttctt
     2221 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
     2281 ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca gcaactactt
     2341 caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta tatttttctg
     2401 gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg tcagccacct
     2461 ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct tgcttaggta
     2521 ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac cctaaaattc
     2581 tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag ccttccatca
     2641 tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat ccagctgcaa
     2701 tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac atatgactag
     2761 catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca gtgattaatt
     2821 cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta aaatgttttg
     2881 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
     2941 ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta ttaatactaa
     3001 aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
     3061 aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa actttatcag
     3121 ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
     3181 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca gaaactctcc
     3241 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
     3301 cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca attaatagag
     3361 gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca gcagttcctg
     3421 gattatatcg tacagctcca atatggtact gattgaccat attactcaag taatttttat
     3481 cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa gtcatagaat
     3541 caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt ttttttcgta
     3601 aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag
     3661 tatctatata tttttgttca atatctacat aatctcccga taaacattta aataaaacat
     3721 cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga taaaagaaaa
     3781 ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat
     3841 ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt cctttatctt
     3901 gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct tcttgaacag
     3961 cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag
     4021 tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc tgttgattga
     4081 cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat tctatttccc
     4141 tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt aatcgattaa
     4201 tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac
     4261 gagtctctga atataaatta atattattcc aatcctgtag gaatttttct ctatgatgaa
     4321 catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa gtcatcgtat
     4381 ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt ctctcctatt
     4441 tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat aatggataaa
     4501 ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat gttgaagcgt
     4561 tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca tagcctataa
     4621 tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt ctagcaaaag
     4681 aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct tgatcatcaa
     4741 taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc atatagccaa
     4801 ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat aagatcaaat
     4861 atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca tattttttac
     4921 ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg ataaaattca
     4981 ttaaaactat tccctgtgca tcattatagt aatcaggaaa tacataatta ttcatactca
     5041 taattaataa ttgagcaagg agtaagaaaa tagccgcacc aattatagaa agtaatgccc
     5101 tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga gattggtcaa
     5161 ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt gcacatatcg
     5221 aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga gcgcctgaat
     5281 catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt gctaaagtag
     5341 ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa gtaatagctg
     5401 atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc cataatatat
     5461 acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata gaagtttgat
     5521 acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac ggtttaaact
     5581 taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca tcagtaactg
     5641 catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt atccccaata
     5701 cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa tcgtttccaa
     5761 aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc atacaattct
     5821 ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac tctgaagcgt
     5881 aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct cctaataatt
     5941 ctttatctca taaaacttat ctatatattt actagctact cgacttactt cttcaaaatc
     6001 acctttttca acagaagcca ataaattccc acctacacca actgcaacaa ctcctacttc
     6061 aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat ttacgtgtgg
     6121 taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg ggaatagttt
     6181 aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca tacaacctgg
     6241 taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa aagatggact
     6301 aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat ctaaaaccgt
     6361 accggctccg attattacat ctttttgatc agcatatttt ttagcaattt ctttgattat
     6421 ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac ccgcaataat
     6481 tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg caatgactcc
     6541 agccgcttct aacttactta aaatttctac ttttttcata tctatctcct tcctttatgg
     6601 atacatactt tgtctagctg tactaactta caaagaataa atttaagctt ttatttcaag
     6661 tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt acgataacaa
     6721 atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc tgctttaacc
     6781 cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg atagcttaga
     6841 aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg ttgtgccatc
     6901 gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc acatcccaat
     6961 tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag tggaagccaa
     7021 tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt ttcagcggta
     7081 tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga atcaccaccg
     7141 acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc atttaaatgc
     7201 atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg aatacctgta
     7261 acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc ttttattaac
     7321 agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa accgtgatct
     7381 gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc ttgcttcaac
     7441 ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat atagttcgaa
     7501 aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa taccttgaaa
     7561 gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg atcatccgtt
     7621 gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata ggaatccgtt
     7681 tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga aattgtcaaa
     7741 tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg attacctggc
     7801 gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc tggaccactg
     7861 atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg atctccagct
     7921 cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc tagcaaatat
     7981 gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa tttatgaaaa
     8041 agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat atttaaacac
     8101 tcctttcctt ccgttagttt ttagaagaga aaatttttcc tctaaataaa ctcccttata
     8161 taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat aaactgcacc
     8221 tagagctcgg tcatataatc cataaccagg tctaccggtt tctccccaaa tcatccgacc
     8281 atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta atatcttata
     8341 catatctaga gaaccatatt ctgatttatg cggcgcttcg taaaaatcga gtccattgtc
     8401 acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt gacaaaattc
     8461 agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag tacataatgt
     8521 aataccatta gaaggagaat ccactacatt acacaaccaa tctaaatcgt cacgattttt
     8581 aacaatacga ggtaatccga aaatggaata tggtggatca tctgggtgaa ctgccatttt
     8641 aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa agttctctcg
     8701 taatttctct tcatcaactt ctttataggc ttctaataat tgcttaattt gtgataaccg
     8761 atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt ctctaataat
     8821 ttcctccgga tcagtaggga tattttttga aatataggct aacgtagaag acccatcatc
     8881 taaacgataa tccaaatccg attttaccca atcaaatact ggcataaaat tataacagat
     8941 aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct caatgtattg
     9001 atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca ttaacactct caataacttt
     9061 caaggtaagt ccattggctt cgacttcatc ttttaaagat ttaattcgtt ctctcggcca
     9121 aacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg ctgggatctg
     9181 acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa aagtcatttc
     9241 catagttact ttcctccaac ttctgttata tatttattga ttgttttacg aactgcttga
     9301 ggacctacta gcatttcgca aaagattttt tcagttttct ttcctacccc aacttgatat
     9361 aaatctaaac caaagatttc tttattttct aaaatttcct gtaccgcaca atgaatcttt
     9421 gaaggatctt gttcacctaa tttcaatact gataatttat cctgcaactc taccaataac
     9481 ggatcaggac ttggcacaaa attttcccct tgatcatcga ctgccagtaa ataacgtaac
     9541 caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc tttttcgaca
     9601 tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc tgttgcaatt
     9661 cgttgtggtg tatctggaat atagggatta ggcaaacgtt tattaattac ttcatcaata
     9721 aattgttttg gatcaataat gcctggattt tcaactactg gtaaaccttc taaataacct
     9781 attcctttga ttaaaccaac caaatcatca tcctgcatct ctgctgaaat tgattgatat
     9841 cctagcagac aacctgttac tgccaaagca gtatgaagtg gattcaagct tgtcgtaacc
     9901 ttcatgatgt ctgctttatc aaccgtctct ctatctgtca taatcacacc agccttttcc
     9961 aatttaggcc ggccatttgg aaaactatct tcaataacta aataatgaac tacctcagta
    10021 ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa atcctcgagc
    10081 cccatcatca atagatcttc ctttacattc tctgaaggat ttggcgtaat tcgatcaatc
    10141 atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa tttttcagaa
    10201 acatatcctt ctttttgcca agcttctgca atcgtaaaaa tactatcttg aaaacgttga
    10261 ccattctgtg agaaattatc agtagaaacc atcgcaatcg gcaactctcc agcattaaat
    10321 ctgctatata acaaggatgt tacaatactc atcgtatgtt gaggattgct aggtccagat
    10381 ttgatatcag ctttgactac cgaaaagaat tgccctttag aatcttttaa actgtaacct
    10441 ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat cttcaaaata tttaaccact
    10501 ctttgatagt ccgctatatt cttcggattg caatataaac tatcagccgt tactgccaaa
    10561 agtctttttt tgagttcacc atctccactc ataacaattt ccaaaatgtc attgttatag
    10621 tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac tactcctgtt
    10681 tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc tcgataaaga
    10741 tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt aacttctttg
    10801 acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga attttgccaa
    10861 tttttaatcg aaaccataat taacctcctc aaactgatat gttagttttg atacatttta
    10921 tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg gaataaactt gcttttttct
    10981 accatatcag aatgattgta ttttttattc agaaaaataa aatactcctt ctttttggac
    11041 tgattttatc gaaacttatt ttttcttttt ttcatgtatt cttttatttt tataactagt
    11101 gtattacttc aaaatttgat attatatggc atgaacagaa ttaaaatatc agttaaaata
    11161 aaagttaagg agcattgtgt atgacaatta aaactagtct ccaaaatcaa gcttaccaat
    11221 ctattcgccg acaaattatc tatgctgact tagaacctgg aaaaaaagta tcagaaaaaa
    11281 aattagaaga aacactttct attggtcgta caccaatacg tgaagcactg attcaactcc
    11341 gacaacaagg tcttgttgat acaattcctc aaagcggaaa ttatatctca ttaattgatt
    11401 tagattcggc caaaaatgct cgatttgtca gagaacattt ggaacgacaa attatgttag
    11461 agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt agaaactata atttcagaac
    11521 aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt tcataatgat aatttattcc
    11581 ataaagcttg ttttgaaatt gcagagcgag aagaagtatg gcaatggtta gaaaataaca
    11641 atacacactt agaacgattt cgatggctac gagtaaaaac ggaaggatta aagtgggaaa
    11701 aaattatgga acaacattat caactatttg aggcattaac aaaacaagat ccagaagaag
    11761 ctaactttct aacatcgttg catcttcatt taatgcttaa tgag
//
LOCUS       DAZAJZ010000088        11759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000088 DAZAJZ010000000
VERSION     DAZAJZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.089"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1153
                     /locus_tag="ACG86D_002311"
     CDS             137..1153
                     /locus_tag="ACG86D_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3141436.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1294..2595
                     /locus_tag="ACG86D_002312"
     CDS             1294..2595
                     /locus_tag="ACG86D_002312"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN3141437.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2641..3303
                     /gene="deoC"
                     /locus_tag="ACG86D_002313"
     CDS             2641..3303
                     /gene="deoC"
                     /locus_tag="ACG86D_002313"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN3141438.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3324..3722
                     /locus_tag="ACG86D_002314"
     CDS             3324..3722
                     /locus_tag="ACG86D_002314"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN3141439.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3788..4870
                     /locus_tag="ACG86D_002315"
     CDS             3788..4870
                     /locus_tag="ACG86D_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3141440.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5082..6644
                     /locus_tag="ACG86D_002316"
     CDS             5082..6644
                     /locus_tag="ACG86D_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141441.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6637..7770
                     /locus_tag="ACG86D_002317"
     CDS             6637..7770
                     /locus_tag="ACG86D_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3141442.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7770..8726
                     /locus_tag="ACG86D_002318"
     CDS             7770..8726
                     /locus_tag="ACG86D_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3141443.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8825..9991
                     /gene="deoB"
                     /locus_tag="ACG86D_002319"
     CDS             8825..9991
                     /gene="deoB"
                     /locus_tag="ACG86D_002319"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN3141444.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10015..10833
                     /locus_tag="ACG86D_002320"
     CDS             10015..10833
                     /locus_tag="ACG86D_002320"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN3141445.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10850..11557
                     /gene="deoD"
                     /locus_tag="ACG86D_002321"
     CDS             10850..11557
                     /gene="deoD"
                     /locus_tag="ACG86D_002321"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN3141446.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         3711 a         2105 c         2737 g         3206 t
ORIGIN      
        1 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
       61 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
      121 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
      181 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
      241 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
      301 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
      361 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
      421 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
      481 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
      541 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
      601 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
      661 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
      721 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
      781 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
      841 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
      901 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
      961 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
     1021 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
     1081 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
     1141 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
     1201 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
     1261 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
     1321 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
     1381 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
     1441 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
     1501 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
     1561 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
     1621 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
     1681 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
     1741 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
     1801 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
     1861 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
     1921 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
     1981 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
     2041 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
     2101 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
     2161 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
     2221 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
     2281 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
     2341 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
     2401 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
     2461 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
     2521 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
     2581 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
     2641 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
     2701 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
     2761 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
     2821 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
     2881 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
     2941 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
     3001 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
     3061 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
     3121 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
     3181 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
     3241 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
     3301 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
     3361 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
     3421 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
     3481 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
     3541 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
     3601 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
     3661 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
     3721 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
     3781 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
     3841 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
     3901 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
     3961 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
     4021 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
     4081 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
     4141 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
     4201 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
     4261 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
     4321 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
     4381 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
     4441 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
     4501 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
     4561 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
     4621 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
     4681 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
     4741 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
     4801 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
     4861 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
     4921 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
     4981 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
     5041 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
     5101 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
     5161 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
     5221 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
     5281 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
     5341 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
     5401 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
     5461 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
     5521 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
     5581 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
     5641 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
     5701 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
     5761 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
     5821 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
     5881 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
     5941 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
     6001 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
     6061 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
     6121 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
     6181 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
     6241 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
     6301 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
     6361 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
     6421 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
     6481 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
     6541 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
     6601 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
     6661 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
     6721 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
     6781 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
     6841 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
     6901 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
     6961 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
     7021 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
     7081 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
     7141 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
     7201 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
     7261 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
     7321 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
     7381 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
     7441 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
     7501 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
     7561 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
     7621 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
     7681 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
     7741 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
     7801 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
     7861 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
     7921 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
     7981 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
     8041 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
     8101 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
     8161 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
     8221 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
     8281 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
     8341 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
     8401 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
     8461 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
     8521 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
     8581 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
     8641 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
     8701 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
     8761 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
     8821 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
     8881 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
     8941 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
     9001 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
     9061 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
     9121 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
     9181 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
     9241 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
     9301 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
     9361 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
     9421 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
     9481 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
     9541 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
     9601 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
     9661 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
     9721 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
     9781 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
     9841 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
     9901 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
     9961 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    10021 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    10081 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    10141 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    10201 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    10261 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    10321 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    10381 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    10441 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    10501 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    10561 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    10621 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    10681 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    10741 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    10801 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    10861 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    10921 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    10981 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    11041 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    11101 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    11161 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    11221 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    11281 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    11341 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    11401 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    11461 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    11521 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    11581 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    11641 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    11701 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatatt
//
LOCUS       DAZAJZ010000089        11755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000089 DAZAJZ010000000
VERSION     DAZAJZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.090"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACG86D_002322"
     CDS             <1..177
                     /locus_tag="ACG86D_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3141447.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACG86D_002323"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACG86D_002323"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN3141448.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACG86D_002324"
     CDS             1199..1771
                     /locus_tag="ACG86D_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3141449.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACG86D_002325"
     CDS             complement(2023..2280)
                     /locus_tag="ACG86D_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141450.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACG86D_002326"
     CDS             complement(2406..3221)
                     /locus_tag="ACG86D_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141451.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACG86D_002327"
     CDS             3738..5063
                     /locus_tag="ACG86D_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN3141452.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACG86D_002328"
     CDS             5056..5406
                     /locus_tag="ACG86D_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141453.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="ACG86D_002329"
     CDS             5388..5573
                     /locus_tag="ACG86D_002329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141454.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACG86D_002330"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACG86D_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN3141455.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACG86D_002331"
     CDS             complement(6724..7677)
                     /locus_tag="ACG86D_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3141456.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACG86D_002332"
     CDS             8289..9782
                     /locus_tag="ACG86D_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN3141457.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACG86D_002333"
     CDS             9917..10213
                     /locus_tag="ACG86D_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN3141458.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACG86D_002334"
     CDS             10314..11165
                     /locus_tag="ACG86D_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141459.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACG86D_002335"
     CDS             11186..11398
                     /locus_tag="ACG86D_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141460.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4041 a         1811 c         2179 g         3724 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DAZAJZ010000090        11619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000090 DAZAJZ010000000
VERSION     DAZAJZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.091"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..356
                     /locus_tag="ACG86D_002336"
     CDS             81..356
                     /locus_tag="ACG86D_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141461.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            783..1238
                     /locus_tag="ACG86D_002337"
     CDS             783..1238
                     /locus_tag="ACG86D_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3141462.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(1318..2067)
                     /locus_tag="ACG86D_002338"
     CDS             complement(1318..2067)
                     /locus_tag="ACG86D_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN3141463.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(2068..2394)
                     /locus_tag="ACG86D_002339"
     CDS             complement(2068..2394)
                     /locus_tag="ACG86D_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3141464.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            complement(2542..2982)
                     /locus_tag="ACG86D_002340"
     CDS             complement(2542..2982)
                     /locus_tag="ACG86D_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3141465.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            complement(3063..4460)
                     /locus_tag="ACG86D_002341"
     CDS             complement(3063..4460)
                     /locus_tag="ACG86D_002341"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3141466.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            4612..5883
                     /gene="celB"
                     /locus_tag="ACG86D_002342"
     CDS             4612..5883
                     /gene="celB"
                     /locus_tag="ACG86D_002342"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN3141467.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6010..6729
                     /locus_tag="ACG86D_002343"
     CDS             6010..6729
                     /locus_tag="ACG86D_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3141468.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            7107..8081
                     /locus_tag="ACG86D_002344"
     CDS             7107..8081
                     /locus_tag="ACG86D_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN3141469.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            8134..8739
                     /locus_tag="ACG86D_002345"
     CDS             8134..8739
                     /locus_tag="ACG86D_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3141470.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(8833..9585)
                     /locus_tag="ACG86D_002346"
     CDS             complement(8833..9585)
                     /locus_tag="ACG86D_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN3141471.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(9599..9859)
                     /locus_tag="ACG86D_002347"
     CDS             complement(9599..9859)
                     /locus_tag="ACG86D_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141472.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            complement(9881..11242)
                     /locus_tag="ACG86D_002348"
     CDS             complement(9881..11242)
                     /locus_tag="ACG86D_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN3141473.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            complement(11315..11614)
                     /locus_tag="ACG86D_002349"
     CDS             complement(11315..11614)
                     /locus_tag="ACG86D_002349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3141474.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
BASE COUNT         3574 a         2360 c         2015 g         3670 t
ORIGIN      
        1 tatttcagta tccttcttgt tttcatataa ccaaaggcgc tatgatttcc atatgaaaaa
       61 aataaagtca ggtgaaaaaa atgaaacttg aggataaaaa agtcattttg aagaaaatca
      121 aacgactacc agaccaaata gatggctata cgatctgttc tttcaaaaaa gacaggagta
      181 taaaagctta tacgcaaaac acagaaaaag gaataatcta tttcctacat gaagaaggtt
      241 acgataccca aacctttatg tttactgaaa aaaaagaatt tcataaacag atgaaaaaac
      301 tgattgaaaa agaatttcca agaagtcata aactctatgt gaatcagcaa ttttaacttc
      361 taactgttct ttattaatta aaaatgttct gtaataaaat aaaactttat aacaactata
      421 acaaaaccga atgcaaaaca gaagttctat aagttataat catagttgtt aggtgattca
      481 tctagtaaaa acaccagatt tcaaatttca cacaatttaa actagatcaa tcatcaacct
      541 tcttcaaatt gttagaaccc tttcccgagg agctgcagca taagcaaaaa aacaaacagg
      601 aagccaaatg gctaagacat tttttctatt agtctagttg cttctcgtcg caagtttaag
      661 actttagaaa gttatttcta atgatgtttg ggttgttttg atcaatgctt atgcacagct
      721 ccttacctat tatttatctc aacagatcta acaaattaat ggactattta aggagttttt
      781 ttatgcttac gatctattct tctcgcacac atagttacca tctgatggca aactggctga
      841 aagaccatca aatgccttac agagaacgaa ttttcacacc taaacagcct ttaacaagga
      901 aagagatatt ttatttattg tcattaacag aaaacggatt tgacgatcta ttagctaaac
      961 gctcgattga ttataaaaat ttgaaagacc aaatcgacca atgtacgacc tcagagcttg
     1021 tcgatctgat cgtctcgcat tttactttgt tacaacgtcc catcattact gacggaaaga
     1081 aattattagt cggattcaat gaagatagca tgcgtctttt tatccctgta caatggcgca
     1141 gaagtcaact gaaatctgtc tataacgatt ttacatgggt aagcaatcta gaaaattcta
     1201 cggatttaga tgatgaattt ttaaattatt atatataaaa aagactgtga caaaagcgtg
     1261 tcacagtctt tttttctacg ccggataaat agaatatttt tacaaatctg aatacattca
     1321 ccagtaataa gcgctttgag tgaattgacg ataagaatga cggactagac tgccataaat
     1381 gatcaagaag acacccgttc ctaccgtaac aaaaaatata gaggcaccga tactaccatc
     1441 catataccac attaaagaag ccaaaagtgg gaaaagtgaa aaaatacata atccaattcc
     1501 tgcaatcagt gagaagccaa aacttcgctg atactcttct tctttttctt ttgcttctgc
     1561 taatactttt tcaggtattt tccgatggtg caaaaagtga tttaaacgtg tgattttcat
     1621 accagaagca ataaaaaaac cgacagctaa tgctataccg aaaatcagac aagaaatacc
     1681 catgaacaca tatcctgctg aacaaaagaa aagaaaactt gcaaaagata agctaatgaa
     1741 tagaacacct gatgccactt ggagacttgc cgctctgtat tctttccagt agttttctgc
     1801 ttcgcttaaa taaatagaag ctgtgtttgt ttccgtctcg tcagcttgat gttttttttc
     1861 gacatctagt tcttctaata attcatcgat tgaaccaaat gtcgaaatta ctgtaccgat
     1921 ggcctcctgt tcattttttc cttctcccat caattcatga taatggtctt ccatatttgc
     1981 caacaaatca gttttcagct tatctatctc ggtactttga ggtactccga taaaaagact
     2041 gtctaaataa ttacggattg tatccattta atttccctcc aaaaattgat cgactacgtc
     2101 tttcgtcgtc agccactctt gctttttttc ttctaaaaga ttacgcccta tttcagtgat
     2161 tttataatat gtccgctttt ttccatgagt cacttctcca ggatacgaaa caatcagttg
     2221 attcttttct aaacgggcaa aaacggagta aagtgttgtc tctttgatca cataggaatc
     2281 atttgtcctc tcacgaatcg ttttagaaat ttcatatcca taagaatcgt ttttgttaag
     2341 gatcgataaa ataatcgcat cattgtatcc gcgtattcca tcacttgaaa tcattttctt
     2401 tcctccatat tactccatct gtcgttgtaa ttagaatagc atgattactt catcaggtaa
     2461 agtaattttt tacaaaaaaa taaaaggaac agaaagcaaa agaagcgtta tgcttttttg
     2521 ctttctgttc ccttaattta tttattttac aaccatgacg ttacatgatg cgtggttgac
     2581 tacataggaa gtagtggaac ctactagtgc acgttctacc gcattcagac ctgtcgcccc
     2641 catgacaatc agatcgattg gttctttcga ttcatggaaa ttagcaatca gtgtctttgg
     2701 ataaccgtaa agaacatatg tctcgatatc tgtcaggcct ttttcaattg ccaagtttcg
     2761 atgttctttc atctggtttt ccgcaacctc atccaattct tcctgaaggc ttgttgtaac
     2821 aaatgcaaaa tcaccaaagt aattgcctgt tacttcattg acatacagga tgtgcaacgt
     2881 tgcctgattt cgtttggcaa tcgtgattgc ctcaagaaat gctttttctg ccttttcaga
     2941 accatcaatt gctactaaaa tattacgata agtatctacc attgtaaacg cctccttcac
     3001 tgttagtgta gcacgattac agtgttagaa caaacatttt gtgaacgttt tatcaaaaaa
     3061 tatcaggcat caaatccgtt tttttcagag accgttttaa accagcgacc tgattttttg
     3121 atcgtccgtt taccgtcttg tgccaaatca acagcgataa agccatagcg attcttataa
     3181 gcatttgtcc aagaccagtt gtccatacat gtccacatgt ggtaacccac acaattggtt
     3241 ccttcttgga ttgcttgatg gacgtatttc aaatgatccg taacaaactc gatccggtaa
     3301 tcatcttcaa taattccttc gctgttaaca aatcgttctt ctccttcgac ccccatccca
     3361 ttttcagaaa tatagcattt gatattacca tagttttctc ttgtattcgt taaaatatca
     3421 tagattcctt tttcgtaaat ttcccatcca cgatatggat tcattttttt attgggcatg
     3481 tcgtaattat caaaataatc atctggcatg gggctatgtt cgtgattgat tggagtttct
     3541 tttgctttga ttcgacgagg ctgatagtaa ttgattccta gcaggtcaat cgtgttttca
     3601 cggatgagtt ctaggtctcc ttcatggatc tctggcatta tagccaattc tttgacaatt
     3661 tcgatcaact cttgtggaaa ctctcctttc actgcagggt caaggaatga tcggttgaag
     3721 aatgcgtcag cgatcttcgc cgcttttaca tcttctgggt tcgtttcatc tctcggatag
     3781 cttggtgtca gattcaagat gattccgatc tcaccatcat gtccgccttc atggtaggct
     3841 ttgattacct ttgcgcttgc tagattctca tgatagccta cttggaccgc ttctttcata
     3901 ttgatatgat ttggatagtg gaattgatac aagtagcctc cttctacagg tacgattggt
     3961 tcattatgag taaaccattt tttgaccaga tctccaaaca gttcaaagca cgctgctgca
     4021 taagaaacat aagcgtcgac cgtttctctt gataaccatc cgcctttttc ttgcagtggt
     4081 agtggcatat cgaaatggta aaggttcaca aaaggttcaa taccattatc cagcaattca
     4141 gtaaaataat ctcgataaaa agaaacagct ttttgattga tttcacctgt cccttctgga
     4201 atcaaatgac tccattggat agatgtacga aatgtgttat ggccagtttc tttcattaac
     4261 tgaacatctt ctttgtattt tgtgtaaact tgcgaagttt tttcaggtcc tacttgatcg
     4321 aagaattttt caggctctaa ttcataccaa tgatcccaaa tattttctcc tttaccatca
     4381 tcacttactc ttccttctgt ttgcggtccg ctagcagcag aaccccacca aaaattttct
     4441 gggaatgtgt aggttttcat ttttttactt cctctctttt ttcttgatac cgtaatcata
     4501 acgaacccta aaaaaaaaac aatacttttt atgaaaatga ctagacattt aaaatttttg
     4561 gcgttatact ttgtatgtaa tcgattgcaa ataaatttga ggtgaataaa aatgaacggt
     4621 tttatggaca agctttctga gaaaatcatg cccttagcta atttattagg gcaaaatcgt
     4681 taccttacag tgctgcggga tgctttcatg ctttccttcc ctttaacgat gttcggttcg
     4741 atcgttgtcg ttatcaataa cttgcctttt ttcagtgacg caacaaaggg aactttatcg
     4801 aatttattcg gtaatgggca aaatgcaaca atgagcatca tgtcggtttt tgttactttt
     4861 ggtatcggct attatttatc aaaatcttat gacgttgaag gtattttcgg tggtgccgtg
     4921 tcttttgcca gcttcttgat tcttacgcct tttgcaatga aaaccgctgg cggtgaagat
     4981 gtgactggtg tgctttcact tgatcgtctt ggcgcaaaag gaatgttcat tggaatgatc
     5041 gctgctttct tggctggaga aatctactgc agaatcacga aacgcggctg gcagatcaaa
     5101 atgcctgatg gagttccgcc tgcagtaaca aaatcatttg ccgctttgat ccctgctgta
     5161 gtgacactaa ctattttctt agtaatcaat gctgtgatga ctggtatttt caatgctaac
     5221 ttacatgacg tcgtttatga agtgatccaa aaaccattaa ctggtttggg aagtagttta
     5281 cctgcaacat tgattgcttt gttctttgtc caattcttat ggttcttcgg acttcacgga
     5341 caaatcatcg tcaactctgt aatggacccg atttggaata cactgatgct tgataactta
     5401 gaagcttatc aacatggaaa agaattacca cacatcatca caaaaccatt catggaaacc
     5461 ttcactgttg gtattggtgg ctctggaatg actttagctg tcgttttatt gatggccttt
     5521 gttcttaaaa agaaacagta tcgtgatgtt ggacgattag ctttagcacc tggtattttc
     5581 aatgtaaatg aaccagcgat cttcggtcta ccgatcgtat tgaatgcaac aatccttatt
     5641 ccttgggttg tggcacctct aatcgtaacg acgttcaatt atttggtaat ggctgctgga
     5701 cttgtccctg caccaactgg cgtctctgtt ccatggactg tgccaatcat tgcaagcggt
     5761 gtcttagcaa caaattcatg gcttggtggt atgctgcaag tcatcgactt tgtcatcgtt
     5821 gcctttatct ggtacccatt cctgaaagta ctagataaac aaccagattt agatattgct
     5881 taatagatca ttaaggggtt gtgacaaaaa cttgtcatga cctctttttt cgaataaaca
     5941 atgttccaga tgctacttcg agttgtcgga agcataatct ttcgttatac tctagaagag
     6001 gtgaataacg tgccaaaata cgaagaaatc gcaaatattt taagagaacg tatccgttca
     6061 aaagagtatc ctctcgatag ttttttgcct aatcaagtcg acttggtaaa agaatttggt
     6121 gcgagccgga tgacaatcaa aaaagccatc aatatcttag caatggaagg acttgtctat
     6181 tcgcagcgag gatcaggaac gaaaatcttg aatcatccat ttatgcaaaa agacacatcg
     6241 cccgctaacg agtatgaagg tttgagtctc caaatgaaac gcagacatcg ttctttgaag
     6301 agtcaaatca ttcattttga tgttgaattc cctaatcaat atatccaaga acgattgatg
     6361 atcgatgccg agcaacctgt ctacaatatt caccggttaa ggatcttaga tggcgagcca
     6421 tatgttttag agcatacctt tatgccagtc catcttgtgc cgggtttaaa aaaagaacat
     6481 ttgttagcct ctatctatga tcatcttcat caagaattga atgtccattt tgcaggggct
     6541 tatcgtgcta tttctgcaga taaaagtgat gaatatgatc agcaatattt gaactgtgca
     6601 gaaaacgatc ctgtactgga agttcagcaa gtcgtctatc tcaaagatgg acagccgatt
     6661 gaatattctt ccagccgaaa ccgatttgat gttcggaact attctgtgct ggatgtgcgt
     6721 tcaacttaac caaagaggtt gtgaaaaagc cgtttaccaa gagatcgtga cagtggtaag
     6781 cagtttcaag taacaagaag agatcgtgaa atcaacattc tattctatcc taagcattag
     6841 ctcaaaaatg aaaaaataac tcagcataaa aagacttatg accacccgta atgattctaa
     6901 agttagcttt ttaaagctag tttttttgaa atcgatacag ctagtcgtaa gtctttttat
     6961 tttattgatc cgtccctcct cttttccatt cttgattcct tcctgatact ttagaagaca
     7021 aattaattgc aaaatagaga tgcaccctct atactatgtc taaatcaact acttactttt
     7081 tgtaagttaa caggaggaaa tttatcatgg atacacaaat cagaggaatt caccacgtca
     7141 cagcaatgac atctagcgct acaaaaatct accgcttttt tacagatatt ttaggaatgc
     7201 gcttaatcaa aaaaacagtt aaccaagatg acatcgaaac ttaccatctt tactttacag
     7261 acgatactgg tgcgccagga acagatatga ctttctttga ttttccaaat cagccaaaag
     7321 gaaaaaaagg aacagatacg atctctcgtg cttctttccg tgtgccaagt gacgcagcat
     7381 tagacttttg gttggaacgt tttgaaaaat atgacgttac tcattcagca atcaatgaac
     7441 gtttcggcaa aaaatatatc gaatttgaag atttcgataa tcagtattat caattgatct
     7501 ctgacgaaaa caatcaaggt gtagcagctg gcattccttg gaaaaacagc aatgtaccga
     7561 cagagtttgc gatcacagga cttggacctg tctttgtccg tgtcagtcaa ttagagcata
     7621 tccgcttgat tcttgaaaaa atcttaggat tcaaagaaac agaccattca ggagatttct
     7681 acttatttga aatcggtgaa gggggaaatg gtgcaagcat cattattgaa catcggacag
     7741 acttgccaaa agcaatcgaa ggatttggaa atatccatca cttagcattg cgtgtagcag
     7801 acaaagaagc attggaatac tggattcaag taatcaataa gattgaattg ccaaattctg
     7861 gtttcgtcga tcgtttctat ttccaatctg agtacttcct agccgctcat cacgtactgt
     7921 ttgaattagc aactgatggt cctggattcc ttgaagatga accagcagaa tcagcaggag
     7981 aaaaactatc tcttcctcca ttcttagaag aacgtcgtga agccattgaa gcttatgtcc
     8041 gtcctttcga tacaagtgat gcaaataaaa aacgtgaata atctcttcat gctacttcat
     8101 ttgcatctat agaaacagaa aagagggaac aaaatgacct acatttttga aaaaggaaca
     8161 agtggcaagc atttgatctt acttcatggc acaggtggcg atgaacattc tttactggat
     8221 atcgctcact ttcttgcacc aaacagtaca ttgctttctt ttcgtggcac ggtccaagaa
     8281 gatgggatga atcgtttttt caagcgtaat ggattgaatc aattcgacct ggacagtttg
     8341 gaaaaagaaa cagataaact ccatgaaaaa atcaaagaaa tcagcgaaaa agaactgata
     8401 ccgttaagtg actggctgct tgtcggctat tcaaatggcg caaacatcgc tgctcattta
     8461 ttattggagc gtgagaccga gctaaataaa gggttgtttt tccatccgat gtctctaggt
     8521 gttcatcaaa gccgttccac attagaggat aaaaccgtct ggatttctta tggtgataac
     8581 gatccaatcg tttcccctgc ttcctttaaa gaattggccg atcaattcga aacacgccaa
     8641 gcaaatgtga ctattcagct aactgtaaca ggtcatcagt tgacgatgga cgaaatcaag
     8701 caagcgaggg aatggacaga ccacctctta actaaataaa agaaaagaac tacgacttac
     8761 ataatacaac ctgaagcaga tatcgatctg gatcagattt tgtgaaagtc gtagtttttt
     8821 gtgtcagata ggttagtctc caccagctgc aataacagat ttattataaa tagccgtttt
     8881 atgtttcaac tcttccaatc ccccgatttt cttacctttt gaataaaaac cgtcagtaat
     8941 ccctaatgct tgaaaaatcg tctgcttgta ggcaatttct gcattccatt caactgtatc
     9001 aaggatttgc agtgctttat ggatagcttc gattcctgat tctccacctg tgttgatcaa
     9061 tacgccatga gagttgagca gcaacatatg cggtaattcg agatgctgtt tttttacata
     9121 gtcgcttgtc aattcagcaa gttcttcaga gcaattaggc tcaaagtcca tgacaggaat
     9181 ctcttttact ttagctgtgg cttctgtcaa attaggcata ggcaacccag tagtcgccca
     9241 gaacattgca ttaggcgcat gagcatgtag gacagctttg atagctggat tagtctcata
     9301 catcgcttca tgcatattga tttcacgagt taaatgaccg actccgtcaa cgattctacg
     9361 cgttcttggt tctactacta gaacctgtgc aggagaaatc tctcctagat aggcttctga
     9421 catcatggtc ggcgtcatga tgatgtagtc tttcccttga tcatcagtgg ttttaaagga
     9481 aaaattccct cctgcgacat ttgtgttttt cctttcgaaa atcactctta cgatacgtgc
     9541 catgtcctgt cgttctctct caaataacat acgtccatct gacataatga cccctccatt
     9601 atctttttat aaaacttttt tctctcaata tttctttggt agcttctttt attttcattg
     9661 attcttctat tttccgtttt cttttttcat cgtactctct gaataaaacg tgatttccat
     9721 atttatagat cactagtgcg aaaaaaatga ccaatagatt gatgatgata ttctgagtaa
     9781 agagtccata tagaaatagc agaagtgtac cacaaagagc gactgtccat tttattttat
     9841 ctgtctcttc tcttttcatt actttcccac catttcaatg ctatttttgt agttcagcat
     9901 aacgttcact cggtactttc gcttttatca tatatttata aagtagaaca aacagtccta
     9961 gaaaaatcac gcatcctaca atagcgagaa tattcctatt aaatgcctcc gcaaataaaa
    10021 ccctgaactc cggcccttct atggatgacc atgtgatcaa ctgtcctttc ttgattccct
    10081 cgactgctcc gcttttttct gctaaatcac tgagtattgg cgcaaggtaa gtggccccgt
    10141 acaaataaat aggcatagtg atgattccca agatcagcat acgagccaag tttcctcctg
    10201 ttaaaagtaa tccgccgacc gcaatcgaat agttgataat ccctgctaat ggcaatactt
    10261 ggtttcctgg caaaataatg gcataaccaa taaacacagg aacaagtaaa atagcagtaa
    10321 cccaaatttc tgaacgacca gctaatatcg gccagtccaa tccgatatat acttcacgat
    10381 ccttaaaccg ctttttcata aattctgaca tagcgtctgc tagaggagac aaagattgca
    10441 tgaacatttt agagatcata ggaaacaaac ttaatgctgt agctgtttgg atcgctagat
    10501 taagagaacc tgaaacgcca tacgcagcag caaagccaag ccccaatcca ataatgaatc
    10561 ccattacaga gttttcagag aagatcccaa ttttcttctt caatgcattc gtatccgctc
    10621 gtttatttaa agcaggaatt ttatccatga tcatattgaa aggcagcaac aagacagcgg
    10681 cagatgtcat aaaatgagtc actgttacta atggaatccc tgtgagatct tgtatatgtt
    10741 tttgaaacat atcaccaagc tttaattcca atataacttg gaccattgca gtaagaaagc
    10801 ctgcagccaa actaccggaa acataataaa ctaaataagc agttaaagct ttcccccaaa
    10861 cattccacat atcaacattt aacgtcttcg tccaattaaa aagtaacagt acaaaattga
    10921 ccccaatcgt tactgcaaaa aatataaaag cataactcca cgaccatgtg atgcttgctg
    10981 cacctgtcca cccaagatca agtgcaggca aactgattcc cgtattttta gccaatgctt
    11041 ctgatgctgg acttactgcg ttagacatat agccaatgac catagacatt ccgctaaaag
    11101 caatccctaa agtcaaagca gacatgaatg ctttttttaa tttcatccct gccaacaatc
    11161 caagtatcaa aataatcagg ggaacaaaaa tggctgatcc taagttcaaa acataattga
    11221 taatggtttg gaaaacttcc ataaataaac cttctttctt tctaaatata gtaaataaaa
    11281 ctatgaacca acttttttat ccaacaatcc tcctttactg tactaactct ttgatactgt
    11341 cataaatagg ttctacaccc attccagtta aaaatggaat tcctggaaat acttcaatac
    11401 ccaaatcttt tgcttgttca agtacttcct catcaggttg tgcgacatac acatagattc
    11461 cagcagttag aagctcattt tgaatcgatt tgtaatcaac tgcttctaca ggaaatgcga
    11521 tgccgtcatc ttctaacata ctttggatct tactagcgat tgtctgactc gttgctactc
    11581 cacttccaca tgcgacaatt aaccgatttt tcatttttt
//
LOCUS       DAZAJZ010000091        11523 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000091 DAZAJZ010000000
VERSION     DAZAJZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.092"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            327..533
                     /locus_tag="ACG86D_002350"
     CDS             327..533
                     /locus_tag="ACG86D_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN3141475.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(735..1736)
                     /locus_tag="ACG86D_002351"
     CDS             complement(735..1736)
                     /locus_tag="ACG86D_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3141476.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1741..3654)
                     /locus_tag="ACG86D_002352"
     CDS             complement(1741..3654)
                     /locus_tag="ACG86D_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3141477.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3822..4328)
                     /locus_tag="ACG86D_002353"
     CDS             complement(3822..4328)
                     /locus_tag="ACG86D_002353"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN3141478.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4488..4928
                     /locus_tag="ACG86D_002354"
     CDS             4488..4928
                     /locus_tag="ACG86D_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3141479.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4954..5982)
                     /locus_tag="ACG86D_002355"
     CDS             complement(4954..5982)
                     /locus_tag="ACG86D_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141480.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(6037..6111)
                     /locus_tag="ACG86D_002356"
                     /pseudo
     CDS             complement(6037..6111)
                     /locus_tag="ACG86D_002356"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6114..6470)
                     /locus_tag="ACG86D_002357"
     CDS             complement(6114..6470)
                     /locus_tag="ACG86D_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141481.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6768..7742)
                     /gene="sgrA"
                     /locus_tag="ACG86D_002358"
     CDS             complement(6768..7742)
                     /gene="sgrA"
                     /locus_tag="ACG86D_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN3141482.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7938..8777)
                     /locus_tag="ACG86D_002359"
     CDS             complement(7938..8777)
                     /locus_tag="ACG86D_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN3141483.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8962..9849
                     /locus_tag="ACG86D_002360"
     CDS             8962..9849
                     /locus_tag="ACG86D_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN3141484.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10443..11137)
                     /locus_tag="ACG86D_002361"
                     /pseudo
     CDS             complement(10443..11137)
                     /locus_tag="ACG86D_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11121..11519)
                     /locus_tag="ACG86D_002362"
     CDS             complement(11121..11519)
                     /locus_tag="ACG86D_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN3141485.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3436 a         2295 c         1726 g         4066 t
ORIGIN      
        1 ttaggttatc atttgtaatt ttatttcaca gttgatatga ttgttatgta aaataaaaaa
       61 gtttaggata catcgaattt ttggggaaat tcttttgggg gtatcctaaa tcttttctat
      121 tatattccca tattttctat tattggtcta gacgtatgat ggtaaacttt gtacctatct
      181 gattgctgaa tcattaattg cttatcatta gccatctttg tttcaagttc gctagatgat
      241 cattttattc acatcctaac aaacttgtca catttataga ttcttaatct atttatcata
      301 caattataaa aaaggaggtg ctgcttatga aagctatcca tatttctttg ctttttttcg
      361 ctagtgcatt gattttattt gctattcatc tttgttccca gccttatatc aataacaacg
      421 gaatattagt ggaaccagca ttcttttgct taccactcgg ctttttgtcc cttcttgcct
      481 ccgcaggctt cggatttgtt gcttttttta aacgatcaaa acaacaaatc tgacttataa
      541 aatttacgta aaagagatcg tgacaataat cgtgtgatat tcccaaattt cgaataaaag
      601 gtgccccttg ccatctctaa ctcctcagtc acaagttaca atattcataa accatgaaaa
      661 gcttgtttat tgaaattgct ccttgtgact tggagctaag tgacagatta caacctcttt
      721 tgcatcataa gactttactc cttgaaaaac attactaact tttgatatgt ttcttgcgct
      781 tgtttattgc tgaaatcaaa ctgatattca tggttcaaaa cagcattttt ctccgcataa
      841 tctacttttg tgactttcac ccccgctttc tcaaatacag aagttacttc ttccatttgt
      901 ttcgtaaacg tatttgtatt tccatccgtt aagaatgtgt tttgcgtcca acgatccgca
      961 tgtctgacca aggctaaact tttgaccttc tctgattgtt caaagtcctt attatcaaag
     1021 taagcccatc cacatttttc ataaagaaag ctagagattt tatcacttac ttgagtaagt
     1081 gcagaaggat ccaacagacc agacaaagaa ataaacttcg taatctgctt gttctccaaa
     1141 gtcggatcaa gatgatcagc ttttttaatt tcttcatttt gaagtgctaa caaatattct
     1201 ccactaattt gtgcacctgc cgaatttcca ccaatcgaaa tattcgacca atctgcttga
     1261 tcttttatct cttttttagc tgcttgaatg gcttcgttca attggataac tggtgtagga
     1321 tgttttgctt ccggtgatcg ttcataatct atatttacga tgatttgctg cgtgtctgaa
     1381 catagatatt ccaaataatc acgacaatca tttttatccc ctcctacata tgcgcctcca
     1441 tgcatccaga aaagaatcgg taattttttc ttttccactt tcggtgtgta gatatccata
     1501 ttattatttt tgtactttga aggataggat atttcttttt ttatatcgat ttgttcgatt
     1561 ttttgctgga aatcatctgg tcgggcatat acttgttctt tttcacttgt gtcaaattgt
     1621 tttctaacaa gcaagctaat cggctttggt gtgatttgat taatcaaaaa tacagtgact
     1681 aaaataacta cgatgataac tggtactgca atcactattc gcttgaacca tttcattcat
     1741 ttaccctctc tttcttcgcc aaatccatgt gataatcgtg ctaacagata aaaatccacc
     1801 tgaataaatg agaagcgaat gtttcttttc tccagttttc ggcaacattc ttttttttgc
     1861 atctgtcttt ttttgtactt tcactgtcat ctgaatcggt tgcgtatcat cacttgattg
     1921 atcctctcga ggttcattct ttcccttatt gtctgcattc tctgtcggat cttgcttttt
     1981 tggtggagct actgggattt ttttgtaata gaaaataata gttggtatcg tctcattaaa
     2041 aatcccttca gtcggtcctt caacatttgc taattcgtat ccttcgaatg tcttttgctc
     2101 tgtcatataa ttttctccat aagctccgta aagctggatt tcgtcctggt ctttgagtag
     2161 acttttattg ttttcatcca catactttac gtttatctga tcatggaaca ctactttcaa
     2221 aaagtccgtt acatgtttcg tgtaggcagc atggtcaact tcctgtgcct ttccatgccc
     2281 tgctccttct actaataaaa gatctttata ccctcctgat aactcatata atttttgcgc
     2341 gttagacaca ggtactacat catctgctgt tccatggata atcaatttag gcaatgaatc
     2401 tgacattcca atcgtaggta gagcagcttt tgtatccaaa tgcatctcct gatcaaaata
     2461 gtattgtttc aaaaggccca taaataaatc ttcatgctct tgatccccaa tgatgtctaa
     2521 ctgatttttt atggcatccg gtagtgcggg ggctactgtc tggctgtacg taaaacgtag
     2581 ttgttcatca attgatgcaa atccgcaatt ttcaataatt cctttgacat tgacaggaat
     2641 atctttcgct aatacagaca tcatcgtagc tgctcccatc gaaccaccat aaagtaaaat
     2701 cttttgagat ggccgctctc gaacttcttg atttatccag taaaggacgt catctgactc
     2761 atattgtcca taggtcacat aatcgcctcc gctattacca gtcgctctgg aatcaacaaa
     2821 taataaatta tacccggcct tataaaaatc attgtattct tctgtaacga tcccgttatt
     2881 ccaattccct cgaaatccgc catggatcat gactgtttta tctgaacctt gatcgacata
     2941 atatgcatat acatccatca ttttgccatc ttgtttgttt agtacagttt gatgaagttg
     3001 ttttgtattt ggatagaagc ctgtgttttc tgtaggcgtt gtcccatcat ctgtacctcc
     3061 aagcatcgac atcaacacac tgttttttgc aaaagcgata tcgtacaata gataagtagc
     3121 aatcagatgt ttgataatct ctttatctac accataatca ttaacagcaa gctcagtgac
     3181 taataactct attaattttg acttgatgtc ttctgttggt ccattaagta tttctgtcat
     3241 ctgtttaccg aagtaattca aatattcttt accattgaaa ttttcacctt taccgatatc
     3301 atcattttct gtcccattct ctttgtcact tactttagca gaagaggatt cgtcgtttga
     3361 catttttgta gctgttgctt cttctgtttg ttggttatct gtggtagcgg tcgtttcttg
     3421 atctgtgcta gtaacagatg tctcaaccgt tgtttcagta tttgtttctg tttgtgaagt
     3481 actcgatttc gtttcatttc cagcatcagt agtttctgtg gtttctacct cccgagattc
     3541 cgattcggaa gaaatgctct ctccatatac tgtccattgc gggacgcttg tacttacagc
     3601 aaataagatg accaacgctc cctttaatac ttgttttgtt ctcaagctat tcactccttt
     3661 ttatgtttct aggagaatta tatgtaaacg caaacatttt ttatacaaaa aataaatggt
     3721 ttatcccata aaaatagaac aggattattt tctcatttta cgtaataaag cagaatggat
     3781 aaaccagtat ccattctgct ttttttaaac gaagaaacaa cttattgctc caaaaatgtc
     3841 agaaatcgtt tatcccaaat tttctcgtaa aaagcgattg tttcctttgc tttcataaaa
     3901 ggattatcat aagttgtcgt tgctcctcta cacatcataa gctggtatcc taatccatga
     3961 gcaaacttca cagtcgtatc tatacaatac tctgcttgtg ccccacataa ttcgatcgaa
     4021 tgacagtcca attcagttaa taaaacactc aaattagtct tataaaatga attcgcatgg
     4081 gtttttctca caaaataact gtgctcagga atagataatt ccgacaatag ctcccatggt
     4141 tttgtttgtg gcactaattg agcatcttcg tgttgaacga aaataactgg tttcccaatc
     4201 gtttcgtatg ttttgattct tttattcact aatgctgtca ctgtttctaa ttcgtgtatc
     4261 ggcgaagaat caaaacatac ccctttttgt aaatcgatca ctactattgc gtcggctgtg
     4321 tttgacatac ttgtgccttc tttctttttt cttgatttaa tagtatttct tatctagcta
     4381 gttccattat acgtgacaac attttcttat tttacacctc taaatggcac ttttcgacta
     4441 tttcttcatg ttattatttc ataaatactt agctattgga ggaatgtatg atgattaaat
     4501 tgtatacagg ttctggctgc tcgtcttcaa gacgcgcaaa acgttggctt atcgtccatg
     4561 atataccgtt tatcgaacag aacttaattc gtcaaccact aaccaaaaat gaatttatcc
     4621 acttactaaa attgacaaac aacggcgtga ccgaaatgct ctctactcat tctattagtt
     4681 ataaaaatct tgttcacaga atagatgaac tcagtttgag tgaactatat attgagattc
     4741 gtacatcccc ttctttattg aaaaaaccga ttatctacga ccaatctgca aaacttttga
     4801 ctggatttag cgaagataaa atcaggatgt tcctttctaa aactaaaaga aagcaagagc
     4861 tcaacgaact cttgctttct ggtcatcttg tcgattttaa agaaaataat ctttttgcaa
     4921 tgtcttaatt ttggcataaa caatctgctt ttcttaatta acgaaatcca atttttccaa
     4981 tatggatcta tttttctcat tcctgatttt tatgatttca gctttcagac gcgcccaatc
     5041 attttttgta ggttgtccat tccagtaaaa tatctgtgta ttcgtttgat catatgcctt
     5101 tttgtcgata ggataatcgc taatgacaat atcaggtaac tgatcagcgg atgcactata
     5161 ttttattggg cgttcagtca tagataatat ccttttcttt gcccataatt cctgtgtttt
     5221 accatacttt gattgaatat agaatatgac aggttgttcg tgttttataa aaatatccct
     5281 gactaacata cagtattgaa aaatcaaccg tctgttcttt tcataataag tattaagctc
     5341 tggaattctg cgagctaccc cactaaaaaa ctgttgcatg attggataca tcactggaaa
     5401 tatatgctga aaatctctgt cttctgctga ttttccaaaa gcatccacct tcttttttgt
     5461 cccaaaaacc cttgaataca aatggatcat tgccaaattg acatatagat aatttttttc
     5521 tgaagttgta aaacaaatct tgaagttttt ttcaaaattt tctacaatag acgattcaat
     5581 ccagccccaa ctttcttgta ctttatctgg agtatccaac aaatcttctt ccaaataggt
     5641 gttcattaca ccaaacataa aataaaggta agcttgctct gttggagtga tgtgattcat
     5701 cgaccagaag tttgcttcaa aaaaaggctc tagtatgtaa tgcgtaaact cttcttttgt
     5761 tatcaatgga tgcacaaatt tgttcatctc ttctgggaca tacgtaatcg agtggccttg
     5821 tttcattcgt tgcaacgaaa ttcttagtag gctgatccat ttctttctat ctatactacg
     5881 cgtatatggc gcatgtttcg caaatttttc taatagttga cgtttatttt ttatacttga
     5941 taatatatca gaatgatttt cgtcgaatga ctgccaatac atcaagtgaa aaaagtatcg
     6001 gatacggtat tcttctcctt ctatcggatt accttttttc aagctgcaag ataaatgata
     6061 gtttttcaaa atagtattaa gttctcttaa ttttctttga atcgtacgca aacttatata
     6121 attttttgac taagattttc taaggattga taagtacctt tgtaaagtga atccagtaaa
     6181 atataaccaa tcgttccctc taaaagtata gaataaactt tactcagtaa aaactgagaa
     6241 gaaaactcga cttttatcat cttgttttct gaaagaaaag ataatttata ttctggatac
     6301 ctttcgcaaa acgattctgc ttgttgaatc accgaattca tcttagccag ggtgtagttt
     6361 agatctaaag cgatatcctt caccacatat aatttctcgt ctctttcata acaatagttc
     6421 aaaaattgat atacttgcct ttcattgata tcaataaatt cttcaaacaa agtacatact
     6481 cctttttatc tattatttag acttattccg acagctacat gaataacgta caaatagatt
     6541 cgttactgat ttttcagaga tactaccatg cgcagtgtgc attgtcaatt attcttgctc
     6601 ttattttcca tttgtgttcc tgttatttcc tgtttctcaa atatgttagt aaacaaaaat
     6661 agatctggca aagctacaac ttcgccaggc aatacagcac tctcgaatga aatattcact
     6721 caaaaatcta tcgttttatt caattttaga tctgttgctt ttattcttta tttcactcga
     6781 tggaagagag aacgaaaata gcttgctcct acaatcaaaa tgagtaacac acccatccat
     6841 gtaattagct tgttcaaggt tctactacca gtttctggta aatcgctttt tctattatct
     6901 gtttctttcg ctttttcttg atgatcaatt ccacgagaaa caaaatcagg ctgttttagc
     6961 acacttttta ggttgctccc tgcatttctc ttttgttcct tagttggtat gataggtacc
     7021 aaatcaagtt cattatttac ttttcctgtg ctgctttcgc tgctcgtgct tcctgtgcta
     7081 ctctcactac tcatgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg
     7141 ctgctcgtgc ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc
     7201 gtgttttctg tgctaatttc actgctcgta ctctctgtac tgctttcacc gctcgtgttt
     7261 tctgtactgc tttcactact tgtgctatcc gtactgcttg gcggtccaac aactgtccaa
     7321 ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat cataaaaaac aatcaaatca
     7381 actttatctt tactcttgat ctcatcaatc gatagttctg cagaaatcat tattgtatac
     7441 tcaataccag cggtccattg tacgtctttt aaataaactt ccccaataac tttttctgaa
     7501 cttccattac catcccaacc atatgttaga acagcatgta catcactagc attcacgctt
     7561 ttgtttggtt tgactggaat agtaatgact tgttgtgtca tgtcataaac aatccgatca
     7621 gttttcacag taaatgaaag attctcattt gcccgttcat ttgcagaagt actgattcca
     7681 atcattgctg caattatact cagcccaaga ataacaaata atacggtcgc tgttttcttc
     7741 atttctatca ctctccatat aattaccttc tatttgataa aggaattata taaaatgaaa
     7801 atgatgagaa cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg
     7861 aatttgttta ctttttcgtt taatcaatga cagacttaaa aaagcaagac cgatatcagc
     7921 cttgctttct tcatctttta ttgataaaca gcagtgtatt tcttcatccg ctttgtttta
     7981 tatgcataga gtaacaaacc aacaatcaag actaacaaga ataatgtagt tgatatagat
     8041 gcttcagccc caaagtttcc cccagtaatt attgtatttt gcgattggat cagagtggag
     8101 aaaaaacctt gttcttgcgt tccgctgact ggtataccca aaatagctcc ttgtgtccaa
     8161 ttccaagtaa tatgagcacc aatagccgta aataaacttc ctgtactgta gataagtgcg
     8221 ctgaaaaagc aacctgctaa aaagatattg aaaactgcca gaaatgtcat gttaggattc
     8281 aatccgtgca aaagagcaaa taatgcagca gatccaatca ttgctattaa cggaccaaat
     8341 ctaagcatct gatgctgaaa aaagccacgg aataaaagct cttctgatgc ccccactgca
     8401 ataaataaaa taaaactcgt tatcagcggc acaatgtttg cgtttcctga atctaaccga
     8461 tacgcccccg tcaaatataa tggactagta ataagcagaa acactccaat tccaacaacg
     8521 agacctcccg ctaaataccc ccagtctttt cctcgaacag aaacaccata atcttctatt
     8581 ctttttcctt cgaccttact agcaagaaat aaagctagta aaaaaacagc taaatgtccg
     8641 accgtaccgg caattgctcc aatccattca ctatttccaa taaacatatc cgcaagtgca
     8701 ttattgacaa gaattcctaa aacaaaacca acaaagacta ttaaaacaga tccaatcaac
     8761 cgaataatcc tgatcatctt tcatccctcc tttatattaa gtttaacaac ccttttcttt
     8821 cttgtctcgt gaaaactatg taaatgttct ccatcataga gagaatagat ttttacgaac
     8881 taagaatatc tacagcaacc ttacgaaaat gaaagactgt ctccctaatt cttctgttag
     8941 actgttttta ataaggaaag gatgggaaat tttttgaaaa aacaactact aattatgtgc
     9001 ctttcagcat gtgcaatttt actattctca ggttgtagca agtccaatga aacgattgca
     9061 acgtttaaag gtggaacact tacagttcag gatttttatg ataacccaaa agttaaatca
     9121 aataatcaaa cactattaaa aaaaatgatt gtcttcaaag catttgatga tgtatacgga
     9181 aaagagattt cttcaaagga agtaacaaaa gaatataatg aacaaatcaa aaaactcggt
     9241 ccaaactaca aggaacaatt aaaagcagtt ggccaaacag aagagacata taagaaactc
     9301 tttaaacaga tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt aacagataaa
     9361 gatctagaca ctgcttggaa agaattctat ccagaagtta gtacacagat cattctattt
     9421 tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa atgaaggaga aaatttctcc
     9481 aagcttgttc aagcttatgg aaaaaataaa acattaaagg aaactgatgg aaaaatgaat
     9541 tttgattcaa ccaatccaga gattcctact gaagtaaaaa aagcagcatt taaactaaaa
     9601 aacggggaag tttctgatat cattcctgta acagatccaa cgacttatca gcaaagctat
     9661 tatctagtaa aaatggtaaa aaaacaggat aaaggttcta ataaagacaa atacaaatct
     9721 gagctagaaa aaattgctac cgaagcaaaa actcttgata cggaattcat ggataaaaca
     9781 atccgtaaag tgatgaaaaa agacaatgta actattaaag atccttatgt aaaaaatata
     9841 ttcaaataaa caaatttgac ccaattgggg atgtggcaaa atttgagaag tgatttttgg
     9901 aaattccttc ttatttttga ggcaaaccac ttttgccaca acttcatcgg ggcctttttt
     9961 tatttgtacc aaaacaaatc actggatttg ttacaatcat gaggatataa tttaaaaaag
    10021 ttttttattt ctatcacatg aatcaatgcg ccattaagta gattctttca tgcttcccta
    10081 ttttttcact tgccgtatcg cttcaaaaac aactatcata aactcaaact cgactgaaaa
    10141 gttattttaa aaaagactaa aaatatccct tctggacttg ttcttcttat tctgttatac
    10201 tagagaaaca cgaaagatat ttttatgcga cacttttcat ttgtttttat gaaatttatt
    10261 caaaactggc gtaacttcag agttacaagg atgtattaga ggttagggaa tacatcgttt
    10321 aaatgagaaa taagtaatag ttagtaatag tcacccaaac tttaaattaa tgaatattga
    10381 atcgtcagat agcctcctcg agttttcgag aaggctatcg tttattttat ggagaaattg
    10441 attcagaagt aatcagcgac cttttttagt tcaccaatcg tttctgcttg gaaagtttgc
    10501 acccaatctt cttggaagcc cctcactgct ttatcaatcg agggcatctc taatacttga
    10561 tctagtagct ctttacctaa cgttatcgct gagctccaga tcgaatggct gtagtgactt
    10621 gttcacaatt tttgaaacta gctgctaata tttttgttgg tctttgcgta cgttcgatct
    10681 cagtccgcaa cgaatcaatc actttcatag catcaatgcc ttgattttgc atcctgttat
    10741 aataaggtgc aatataatct gctccattat taatagctaa atacccttgt aattctgtgt
    10801 aaatagctgt accggtaatc aaataacctt cttttttcaa ctttttgatc gcttgaaggc
    10861 ctgcttgatt caccggcacc ttgataaacg tatcttttcc cagttcttct atgataactc
    10921 gagcatcctc tatcatttct tcttccgttc ttcctattgt ctgaatatgg ataggaatct
    10981 gtccgattat ttttttgatt tctttcatat gcgagaagaa gtttgacgct ccttcttttt
    11041 tcagaattgt aggatttgtt gttacgcctg ttaaattcaa aaactgttga caagaataaa
    11101 tactttttgt atttgctgta tcaagcatga tttccatata gctttccccc attctgatcc
    11161 tcttgatcga ttttaatgat tgtccccttt cttaaatgcg gcatttcctt atcttctaca
    11221 tacattgttc ctggaagttc cggctcatga gatccatcaa attttatcgt gatatgtcct
    11281 aattcattca aattagtttg gacaacatct cctactgctg tgattacata catttgttta
    11341 tcaaaacaga gcttcatatc acaagttatt tcttttctta cctgattcaa ttcaatctta
    11401 taacaataat ctgttaatgt ttccggtgct tcatctccaa ataaaatcat tattttttct
    11461 ttcctgaata cttctgcttc tataccaaca tctgttacaa ccgtttcaaa gatcgtcatc
    11521 tct
//
LOCUS       DAZAJZ010000092        11396 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000092 DAZAJZ010000000
VERSION     DAZAJZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.093"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACG86D_002363"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACG86D_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1426..2100
                     /locus_tag="ACG86D_002364"
                     /pseudo
     CDS             1426..2100
                     /locus_tag="ACG86D_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            2093..2254
                     /locus_tag="ACG86D_002365"
     CDS             2093..2254
                     /locus_tag="ACG86D_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141486.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2738..3334
                     /locus_tag="ACG86D_002366"
     CDS             2738..3334
                     /locus_tag="ACG86D_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFN3141487.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            3315..3761
                     /locus_tag="ACG86D_002367"
     CDS             3315..3761
                     /locus_tag="ACG86D_002367"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3141488.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            4104..4427
                     /gene="relB"
                     /locus_tag="ACG86D_002368"
     CDS             4104..4427
                     /gene="relB"
                     /locus_tag="ACG86D_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN3141489.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            4747..4974
                     /locus_tag="ACG86D_002369"
     CDS             4747..4974
                     /locus_tag="ACG86D_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN3141490.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4990..6156
                     /gene="hemW"
                     /locus_tag="ACG86D_002370"
     CDS             4990..6156
                     /gene="hemW"
                     /locus_tag="ACG86D_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN3141491.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6391..7434
                     /gene="hrcA"
                     /locus_tag="ACG86D_002371"
     CDS             6391..7434
                     /gene="hrcA"
                     /locus_tag="ACG86D_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN3141492.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7528..8091
                     /gene="grpE"
                     /locus_tag="ACG86D_002372"
     CDS             7528..8091
                     /gene="grpE"
                     /locus_tag="ACG86D_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN3141493.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8142..9971
                     /gene="dnaK"
                     /locus_tag="ACG86D_002373"
     CDS             8142..9971
                     /gene="dnaK"
                     /locus_tag="ACG86D_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN3141494.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10122..11288
                     /gene="dnaJ"
                     /locus_tag="ACG86D_002374"
     CDS             10122..11288
                     /gene="dnaJ"
                     /locus_tag="ACG86D_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN3141495.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4004 a         1834 c         2333 g         3225 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatatt
     2641 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     2701 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     2761 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa
     2821 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     2881 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     2941 ttcagagaag tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac
     3001 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     3061 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     3121 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     3181 accaaaagaa cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa
     3241 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     3301 tcaagagcaa ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata
     3361 ttcgtaattt atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata
     3421 aaaaaatggc tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat
     3481 ataaacgtaa tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac
     3541 tttgccaaga aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata
     3601 tagaatattt gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca
     3661 attgtcatgc tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga
     3721 tcgttgaaaa aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa
     3781 taagctaatg tgagctatgc cttctatata agactaagtt ctattataag taacgaagta
     3841 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
     3901 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
     3961 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
     4021 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
     4081 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
     4141 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
     4201 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
     4261 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
     4321 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg
     4381 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
     4441 tcaattcgtt gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac
     4501 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
     4561 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
     4621 taaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     4681 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     4741 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     4801 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     4861 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     4921 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     4981 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     5041 atcccatttt gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg
     5101 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt
     5161 catcctacag cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct
     5221 gctaaacaac ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt
     5281 aatgagttta ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg
     5341 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg
     5401 aaaaaaattg gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa
     5461 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     5521 gaaggatatc gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     5581 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     5641 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     5701 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     5761 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
     5821 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     5881 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     5941 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     6001 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     6061 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg
     6121 aacaatgtgt ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc
     6181 gaaacttgaa aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa
     6241 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     6301 gtctttctct tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga
     6361 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     6421 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg
     6481 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     6541 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     6601 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     6661 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     6721 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     6781 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     6841 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     6901 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     6961 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg
     7021 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt
     7081 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     7141 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     7201 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     7261 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     7321 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     7381 ctagcagcta aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa
     7441 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     7501 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     7561 atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt
     7621 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     7681 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     7741 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     7801 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     7861 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     7921 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     7981 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     8041 aaattacatg atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga
     8101 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     8161 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     8221 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     8281 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     8341 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     8401 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     8461 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     8521 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     8581 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     8641 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     8701 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     8761 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     8821 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     8881 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     8941 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     9001 caaaagtacc agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg
     9061 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     9121 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     9181 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     9241 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     9301 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     9361 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     9421 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     9481 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     9541 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     9601 tgaaagatgc agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac
     9661 gtaatgatgt agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     9721 aagtggacga agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg
     9781 ttgaagcaaa caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     9841 aaaacttgac tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag
     9901 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     9961 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
    10021 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
    10081 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
    10141 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
    10201 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
    10261 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg
    10321 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
    10381 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
    10441 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
    10501 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
    10561 agtataatcg ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac
    10621 aaccaactac ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc
    10681 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa
    10741 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
    10801 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
    10861 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa
    10921 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
    10981 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
    11041 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
    11101 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
    11161 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
    11221 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
    11281 ggaagtagtt tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta
    11341 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctgg
//
LOCUS       DAZAJZ010000093        11388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000093 DAZAJZ010000000
VERSION     DAZAJZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.094"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(560..1600)
                     /locus_tag="ACG86D_002375"
     CDS             complement(560..1600)
                     /locus_tag="ACG86D_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN3141496.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="ACG86D_002376"
     CDS             complement(1949..2275)
                     /locus_tag="ACG86D_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141497.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="ACG86D_002377"
     CDS             complement(2262..3050)
                     /locus_tag="ACG86D_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3141498.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="ACG86D_002378"
     CDS             complement(3418..3708)
                     /locus_tag="ACG86D_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN3141499.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="ACG86D_002379"
     CDS             complement(3844..4023)
                     /locus_tag="ACG86D_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141500.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="ACG86D_002380"
     CDS             complement(4020..4370)
                     /locus_tag="ACG86D_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141501.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="ACG86D_002381"
     CDS             complement(4363..5688)
                     /locus_tag="ACG86D_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN3141502.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="ACG86D_002382"
     CDS             6138..6752
                     /locus_tag="ACG86D_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3141503.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7159)
                     /locus_tag="ACG86D_002383"
                     /pseudo
     CDS             complement(6917..7159)
                     /locus_tag="ACG86D_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7363..8682)
                     /locus_tag="ACG86D_002384"
     CDS             complement(7363..8682)
                     /locus_tag="ACG86D_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFN3141504.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8816..9424)
                     /locus_tag="ACG86D_002385"
     CDS             complement(8816..9424)
                     /locus_tag="ACG86D_002385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141505.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10062..10319)
                     /locus_tag="ACG86D_002386"
     CDS             complement(10062..10319)
                     /locus_tag="ACG86D_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3141506.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10312..10581)
                     /locus_tag="ACG86D_002387"
     CDS             complement(10312..10581)
                     /locus_tag="ACG86D_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3141507.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10712..10891)
                     /locus_tag="ACG86D_002388"
     CDS             complement(10712..10891)
                     /locus_tag="ACG86D_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141508.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            10989..11225
                     /locus_tag="ACG86D_002389"
     CDS             10989..11225
                     /locus_tag="ACG86D_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141509.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3491 a         2058 c         1721 g         4118 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgcgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatcc ctgaataatt catagcttta attctttggc acattttatt
     7201 gaaatatgta tcactaatat ccctatcaac tgattttgac taaaaagtta cttcaaacat
     7261 tcactgttca aaaatattga ggaataaatt ttgggtgtaa ggcattttga gcatacttct
     7321 ccctggataa cttcccagta aaaaaatcgt tagattgttg gattagatcc attgactggc
     7381 tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga tacacatgat aactagataa
     7441 tttgagatag actcgtctac ccgtttggac taatttccca gcaactttca gaaacttcaa
     7501 tcgaatagaa tgaatggtca ttcccttttg gacttcttca aagccaatcg ttcttaagaa
     7561 gttgactaag ttgtaagcta tcaagctgag catcattcgg acatgattct ccaaaaagcg
     7621 aggactgtct gttttgtcaa agtaaaagcc agctttcgct tctttaatga agttctccat
     7681 tgtgccacgt ttggcataga tagaaaagat ggtatcagga gaaacatttt ctgatagatt
     7741 cgtcacgata aattcatgtc gaaagagtag ttcgcccgct tcacgtgttg agcgtataca
     7801 cacgcgacga ctttgtgacc aggattgtgc ttgataagtg gtcgagaagt actgaacttc
     7861 tcgttcttcc cacttttgat tatcgccata aagtactgat ttctcagcta tttgacctag
     7921 tctacgatta ttcttcagtc gaataacata atgactcttt tttgattcac acgaatcata
     7981 cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga
     8041 gtgattatag tgctctaata aaggtgtgag aaactccttt acgccttttg atgtatattg
     8101 atttcctgaa cgtagttcag cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa
     8161 tggatggtaa ccataggttt ggtagtgtgt attataattc gtttgttctt ggtgaccaaa
     8221 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
     8281 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
     8341 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
     8401 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
     8461 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
     8521 gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc
     8581 tttcactagg acgagacccg aatcactcga taattgacca cctgtatgag aaatagtgat
     8641 atttgaattg aattttactt ggtttttgtg taagctagtc atgaaaagaa ctcctttctt
     8701 ttggttggtt tagtcacttt aaccatagca gaaaggggtt cttttttcat cacttaacgg
     8761 gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa
     8821 agtatgaatt attcaggcta tcattaattt tataaaaaaa attaaatgtt tttagcactg
     8881 ccatataaca ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca
     8941 atttattacg tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa
     9001 tattaaagta ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat
     9061 tctcaaatat tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt
     9121 cacaggctgt aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt
     9181 taaggagtat aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt
     9241 gagcttcaga agttatatct ctaattatat agttaacgga attaggaagt gtctttaact
     9301 ctaatgaaat ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg
     9361 attcgtcagc aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa
     9421 tcatttcagc aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta
     9481 tatcagaatc aataaattct atttttttat tactatatcg tcatcattat cttgaccatt
     9541 tatgtttatt gttccctgag tggggagtac ttctttcaaa ttcaagttaa atttatattc
     9601 cattacttct cctccaatat ataaaaatta cttaactgta tccaaattta atatgtaaat
     9661 aaatcattaa tttttagcta aattctatga gaagtttatt tttgttgttt ttgctaatta
     9721 actaaaacta gtacaaaaaa tttaaaatag attattaaac ctattgctta ttctattcag
     9781 tcatatatat aagcgtcatc ctttagtaac taacatccca cactacaata gtattataac
     9841 atatctattc aacttcaaat tgttgaaatt taagccattt atatttactt ttcctcaact
     9901 ttagaaatga ttttactaac cttatatccg taatacgcgt aacaaacaca ttcattaata
     9961 acaatcaaca ctaagctata ttagcttttt aaatgactag ggtaaataaa ataaggatca
    10021 aaaactttta agtttctgac cctttcctta cttccgattg gttaatagtg atcttttgca
    10081 gaataaataa atatcgtttc attttcaact ctatatatca gtctatgttc atctgtaatt
    10141 cttctggacc attttccaga taaatcatgc tttaatggct caggttttcc taatccagca
    10201 aagggggaac gatcgatatc ttttattaac ttgttaatct tttttatatt gcttttgttt
    10261 ccttgctcat gccaataaag ataatcatcc caagcatcat cagaccaagc cttaatcatc
    10321 agattcaacc tcgattaagt catgtgtttt aaatgcacct ttggagaatt gttcatctcc
    10381 tcgacgaatt ttttccatga cgtaattatt agaaagtgtt ctcaacgttt cttgcataga
    10441 atcataatct ctttttgata atacaacaac tgtatcttct acatctttac ttgttacaat
    10501 aagtgtttca gcatcctcat taacttgttt catataacta cgtaaatttt ggcggaaatt
    10561 tgaataagct actgcttcca ttcctttcac cccttattta tttctataaa acaattgtac
    10621 attatattgt acaattaagc aaatgattaa tattctaact tcttataatt aaaagtgcta
    10681 tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt acctcaaatc
    10741 ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg gattcacttt
    10801 tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg
    10861 gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat cgaaataacc
    10921 ctatgaaaaa tatggctgca atcatcttga atagtagact cacaagggct aatacaaagc
    10981 gccaaaagat caaacaaaat accagtattg ccaaaaatac tacgaagttt aaatgcacag
    11041 ctgcagcatg gcgcataagt atttcgccaa caaataggac taacaataga ggaatgattt
    11101 tattcatatg gatctccttc tttctcttag gttatcgggt gttgcccgct tatttcgcac
    11161 cacctaagcg gcgggggcgg ttggagcgat tggtgcgaaa taagaaccat tcccttagtt
    11221 actaggtttt ttgtccttat ttcacacatt ctcacttttc ggtataaatg attattgcct
    11281 ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat tttggatttc
    11341 ttctgtatcg tcgtaagtat aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DAZAJZ010000094        11307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000094 DAZAJZ010000000
VERSION     DAZAJZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.095"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG86D_002390"
     CDS             64..972
                     /locus_tag="ACG86D_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN3141510.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACG86D_002391"
     CDS             972..1592
                     /locus_tag="ACG86D_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141511.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="ACG86D_002392"
     CDS             1596..2045
                     /locus_tag="ACG86D_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141512.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="ACG86D_002393"
     CDS             2045..2539
                     /locus_tag="ACG86D_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141513.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="ACG86D_002394"
     CDS             2553..2984
                     /locus_tag="ACG86D_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141514.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="ACG86D_002395"
     CDS             2986..3123
                     /locus_tag="ACG86D_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN3141515.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="ACG86D_002396"
     CDS             3160..3453
                     /locus_tag="ACG86D_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141516.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..3674
                     /locus_tag="ACG86D_002397"
     CDS             3450..3674
                     /locus_tag="ACG86D_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN3141517.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            3671..4690
                     /locus_tag="ACG86D_002398"
     CDS             3671..4690
                     /locus_tag="ACG86D_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3141518.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            4879..4952
                     /locus_tag="ACG86D_002399"
     tRNA            4879..4952
                     /locus_tag="ACG86D_002399"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4913..4915,aa:Ile,seq:cat)
     gene            complement(5467..5766)
                     /locus_tag="ACG86D_002400"
     CDS             complement(5467..5766)
                     /locus_tag="ACG86D_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3141519.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(5772..6002)
                     /locus_tag="ACG86D_002401"
     CDS             complement(5772..6002)
                     /locus_tag="ACG86D_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141520.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(6438..7004)
                     /locus_tag="ACG86D_002402"
     CDS             complement(6438..7004)
                     /locus_tag="ACG86D_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3141521.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(7004..7960)
                     /locus_tag="ACG86D_002403"
     CDS             complement(7004..7960)
                     /locus_tag="ACG86D_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN3141522.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(7960..8616)
                     /locus_tag="ACG86D_002404"
     CDS             complement(7960..8616)
                     /locus_tag="ACG86D_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN3141523.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            8986..11004
                     /locus_tag="ACG86D_002405"
     CDS             8986..11004
                     /locus_tag="ACG86D_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3141524.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    11074..11280
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3829 a         1982 c         2247 g         3249 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa atgaaaaaga aaattactat tactgcgatg agcctgttaa cggctctttt
     3721 tttattgcca attaacggat ttgcctatac tatcaacaat gaatttaatt tgggcgcaaa
     3781 tgaaggtagc tcacaagtag caaataatca gtatatttta ctgcatgaaa cggctaatga
     3841 aacagcaaca ggacgcaatg aagcgcagta tatgcaacgt tcatggacta gcgcttatac
     3901 tgcttatatt gtgggagacg gcggaattgt ttatcaagtc ggtcaacctg gttatgtaca
     3961 gtacggtgct ggttcgtatg ctaatgccaa cagtcctgtg cagattgagt tacaacacac
     4021 acatgataaa gcaacgtttg agaaaaacta caaggcatac gttgaattgg ctagagattc
     4081 agcaatgaaa tatggtattc cattaacgtt ggacactcct tataaccaac cgggaatcaa
     4141 atcgcattta tgggtaacac aaaacatctg gggcgatcat acagatcctt acggttatct
     4201 ttctgaaatg ggcgtaagta aagaaaaatt agcatatgat ttagctcatg gatttaccga
     4261 tgaaaatcca acaacttctg aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc
     4321 taatcctaca ctgacagatg gaacgaatta cgcccacatt gatcagtttg gagaaatcga
     4381 aaatgcaaat ttgcatgtag ctggatggca cattgctaac tataaatacg agtatatctt
     4441 cattatggat tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
     4501 agcagatgta aatcaagctt ataatacttc tggaaatgtt ggctatcatg tatcttttaa
     4561 catgcgtaac ttccctaata agaaagtcta tgtcatgatg cgggcaacga atgatccaga
     4621 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
     4681 taaacgataa aaatagctcc tcgttgagga gcattacata actatattga caactataaa
     4741 aatcattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aatagtcaca
     4801 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     4861 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     4921 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     4981 ttagaatttt ttgaagagta ttatacaagc taaagctttt cttcattgcc actcaaatga
     5041 gtggcttttt tatgtatcct tttatggatt aatgaaagga tgtttcacat agttatattt
     5101 ctgtatattt gaaaagtttt actttgattt ttaaatagaa agacatttgg gttatattgt
     5161 gagataataa taaagaagag tttaaagcgt tccccaaaaa ccactgcccc ataagtgtgt
     5221 tacgctttaa actcttttat atttgaagcc attaaaaagc ataccatatt tttgaaaaaa
     5281 agtgagaaaa aaggcttata attggagtgg tagttaatta gtgacttatt tttgatttta
     5341 tagcactgat actataaaat atagatatca tcatattaca caatcttaat actaacttaa
     5401 aaaatatctc ctttcataag tatggtgata aaatccgttc cgggctacct ttttaggtag
     5461 cctactttaa tctttgtatc tttctggatc aacgaaagta tactttatat agtcataacg
     5521 ccgatgatcg cttcgagcgt ctagcacatc agtcacgata tcaaacaaaa aatatacgtc
     5581 tttcttcatt ctagttttcg cagcagggat tttaaagtag ttcttattag aatagtagag
     5641 attaattaat aagctatctt cgattgctaa aaagaaaact tctgaatccc acaccttata
     5701 aaaatctttg acaaatctat tcgaaggatc aaatttaaac cataattgcg tctcattaaa
     5761 aagcataacc attactccga tctattttta aacttagtct ctacctctaa tatatatcga
     5821 gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg ttgtttattt
     5881 tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc ttttttactg
     5941 ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa ttcttccatc
     6001 ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag cgatatttga
     6061 ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat atgaatgaca
     6121 tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc attcttttat
     6181 atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa taaagaggtt
     6241 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     6301 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
     6361 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
     6421 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
     6481 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
     6541 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
     6601 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
     6661 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
     6721 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
     6781 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
     6841 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
     6901 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
     6961 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
     7021 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
     7081 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
     7141 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
     7201 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
     7261 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
     7321 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
     7381 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
     7441 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
     7501 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
     7561 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
     7621 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
     7681 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
     7741 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
     7801 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
     7861 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
     7921 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
     7981 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
     8041 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
     8101 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
     8161 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
     8221 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
     8281 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
     8341 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
     8401 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
     8461 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
     8521 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
     8581 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
     8641 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
     8701 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
     8761 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
     8821 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
     8881 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
     8941 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
     9001 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
     9061 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
     9121 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
     9181 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
     9241 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
     9301 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
     9361 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
     9421 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
     9481 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
     9541 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
     9601 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
     9661 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
     9721 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
     9781 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
     9841 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
     9901 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
     9961 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    10021 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    10081 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    10141 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    10201 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    10261 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    10321 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    10381 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    10441 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    10501 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    10561 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    10621 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    10681 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    10741 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    10801 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    10861 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    10921 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    10981 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    11041 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    11101 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    11161 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    11221 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    11281 aaagagcaat ctttttatag gattttt
//
LOCUS       DAZAJZ010000095        11150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000095 DAZAJZ010000000
VERSION     DAZAJZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.096"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..439
                     /locus_tag="ACG86D_002406"
     CDS             <1..439
                     /locus_tag="ACG86D_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141525.1"
                     /translation="LMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGN
                     DHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDA
                     VLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(500..865)
                     /locus_tag="ACG86D_002407"
     CDS             complement(500..865)
                     /locus_tag="ACG86D_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN3141526.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(941..1186)
                     /locus_tag="ACG86D_002408"
     CDS             complement(941..1186)
                     /locus_tag="ACG86D_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3141527.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1256..1510)
                     /locus_tag="ACG86D_002409"
     CDS             complement(1256..1510)
                     /locus_tag="ACG86D_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141528.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1674..1997)
                     /locus_tag="ACG86D_002410"
     CDS             complement(1674..1997)
                     /locus_tag="ACG86D_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141529.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2184..2257
                     /locus_tag="ACG86D_002411"
     tRNA            2184..2257
                     /locus_tag="ACG86D_002411"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2218..2220,aa:Ile,seq:cat)
     gene            complement(2642..3139)
                     /locus_tag="ACG86D_002412"
     CDS             complement(2642..3139)
                     /locus_tag="ACG86D_002412"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN3141530.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3179..3988)
                     /locus_tag="ACG86D_002413"
     CDS             complement(3179..3988)
                     /locus_tag="ACG86D_002413"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3141531.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4322..5179
                     /locus_tag="ACG86D_002414"
     CDS             4322..5179
                     /locus_tag="ACG86D_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN3141532.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5172..6074
                     /locus_tag="ACG86D_002415"
     CDS             5172..6074
                     /locus_tag="ACG86D_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN3141533.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6107..6457
                     /locus_tag="ACG86D_002416"
     CDS             6107..6457
                     /locus_tag="ACG86D_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3141534.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6506..7429
                     /locus_tag="ACG86D_002417"
     CDS             6506..7429
                     /locus_tag="ACG86D_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3141535.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7507..8400
                     /locus_tag="ACG86D_002418"
     CDS             7507..8400
                     /locus_tag="ACG86D_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3141536.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8438..9001
                     /gene="amaP"
                     /locus_tag="ACG86D_002419"
     CDS             8438..9001
                     /gene="amaP"
                     /locus_tag="ACG86D_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN3141537.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9015..9203
                     /locus_tag="ACG86D_002420"
     CDS             9015..9203
                     /locus_tag="ACG86D_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN3141538.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9216..10118
                     /locus_tag="ACG86D_002421"
     CDS             9216..10118
                     /locus_tag="ACG86D_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3141539.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10150..10392
                     /locus_tag="ACG86D_002422"
     CDS             10150..10392
                     /locus_tag="ACG86D_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3141540.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10648..11019)
                     /locus_tag="ACG86D_002423"
     CDS             complement(10648..11019)
                     /locus_tag="ACG86D_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141541.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3944 a         1918 c         2116 g         3172 t
ORIGIN      
        1 ccttatgata aaaaaactat ttttactatc aatattaacg ctagcaatgg caggttgttc
       61 tgactctggc ttagcaactg aactgatcac aaaatcaact gaagccacga cagaggagtt
      121 aaaatgtgga aacgatcaca gtgactacag aggcttaaat cgacgatgca tgggaatatt
      181 aggcagaagc aattgtaagc atcgatgtag taatcgaaga aggtgtagaa gaagcaacag
      241 aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga
      301 agatgcggta cttttaaacc acgtcaggag atgttcattt atgtctaggg aagttggcga
      361 tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag gatgaagata accctaaaac
      421 ttacgacatt ttagcttgat ctaagatggt taaagctttt taatgaagta agaggagata
      481 gaaggctttt ctagttatat taggtaagat aaattccttt ttgctccaaa tttgctaaac
      541 attctgctag ataaacttca tcgtgttccg gataaatcgg cacgttgcgc tctacagtag
      601 gtaaatcaaa aaatgcttcg catgatttgc cacgatacat aggatttttc cactctggtg
      661 ctgggctgta accacgtttt tccatttcat ccatgacaag ctgatgatat tggaataatc
      721 tataaggaga atagtgaaaa acataatcca cagtagcatg tttctttccc cagccatttc
      781 ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa
      841 tcaatttttc atgccataat cgcatagaaa tgtctccttt atatatagat tgttaacctt
      901 agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg
      961 tttctgagcg accaattagg taatcaacgg aaatatcgta aaagtcagcg atatagatta
     1021 attcaatgat gcttggaatt tcttctcctt cttcgtactt acgatatttg aataaaggaa
     1081 tacttaacgc gctagaaaca gctacttgag ataaagatcg gccagttctt aagaaattca
     1141 gacgacaacg taaagtatcc agtaaaatag cttcttcgat actcatttaa tcacctttca
     1201 ttatagtaga atagttataa tgactataga aagctacaca tgcaatcaat agtactcaat
     1261 gttttttatt taaacgtatc aaataaagaa tgacgatcaa taaagacaaa accagtcctg
     1321 ataattcttc tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa actttccaca
     1381 taaagtagat agccggaaga gtcaaaatag ctaaataaaa ccgttctttg ttccaattca
     1441 taagataaac aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa actctcacca
     1501 acgtaatcat gtaacggact cctcaacgat tcatataata aaagataaca tgttattgca
     1561 tacatcacaa atgaaatgct aggaaaataa aagaagaata gatcaatgaa aaatgaaaaa
     1621 gcagaacagt tttctgctga taagcaggaa ctgttccgct gaatgtgtct tatctatcga
     1681 gaaaaaatca atggattgat atctaagtaa ttcaatacat ataaaaagag gacgatcgca
     1741 ttcactaaaa aggaaagaat gatgatccca tagcgaatgg aaacatattt aacaaatact
     1801 tttgtttgaa tacgggcata tctttttcct ttatagctga gcatcatgac aagaagcaaa
     1861 agggtaatca caccttgaaa aatcattcca ataaacagaa ttcctgaggt ttgataattt
     1921 aattggagaa aataagctgc tgccgcaact aaatcgaata aaatcgcaat aaatggcaaa
     1981 ataatcactc ctaacattga gaataaataa aagaataaca ttttttctaa aataagcaaa
     2041 taaaaattaa agtaacaaaa tagttatgac acaagtaaaa gaaataagaa taacaatatt
     2101 attttactta tattttgaaa cttgtgagag tttgtcattc gtgctatagt tgaaaagtaa
     2161 caaaaataaa ttttatttat gatgtacctt tagctcagtt ggttagagca gacggctcat
     2221 aaccgtccgg tcgtaggttc gagtcctaca aggtacataa gaggttgtga aaaagctgcc
     2281 ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt acttggctgt
     2341 tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg tgaaatcagc
     2401 gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
     2461 ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt cgtgagcctg
     2521 ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc acgtaggact
     2581 aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa gatagatatt
     2641 tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg gattctccgt
     2701 gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga aaagcactct
     2761 gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact acttcgatct
     2821 ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa gtttcaggtg
     2881 gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa agaagaggga
     2941 tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt atatcccgtg
     3001 tgaagagaat atattcatta ttttggcgtg ctttagctaa tctttgagca atacgttctg
     3061 ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata tgattttgca
     3121 tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt tattcttttt
     3181 aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt gttacttggt
     3241 ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac ccgctgtaat
     3301 tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc gattcgattc
     3361 caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta ggcgtgattt
     3421 ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga tctctttgta
     3481 gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag ctaagagaag
     3541 gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg aaatgttcat
     3601 aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca tcttgggttt
     3661 ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct tttctcagtg
     3721 tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc ttcatttcag
     3781 caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg gaaaaaatat
     3841 ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca ttttcatcaa
     3901 atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca agcaaggtac
     3961 cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt acatatctat
     4021 tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat gaaactaact tgctttgtca
     4081 ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg ctatatatag tagtttttgg
     4141 tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt
     4201 gtgataaaaa aattttaaaa tattcaatga aaacaaagca tatattttgc tttaaaacca
     4261 aatgaggaat attctagatg tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt
     4321 gatggagaag agatacggcg tagttcctgt ggtaactgct tcagacgaaa attatgctcc
     4381 ttacttgagc gtgatgatcg caactgcatt agaaaattgc aataaaacaa gacgcataaa
     4441 attttatgtg attgacgatg gtctatcaga atatagtaaa gaaggattgg aagaaacggt
     4501 aaataaatat tcaagtaatg caagtatcca attcctgaca gtggaaaaag atatctacga
     4561 agacttccta gtcagtgacc atatcacaac taccgcttac ttaagaattt ctttaccaaa
     4621 cttattagct aaagaggatt ataagaaagt cctttactta gattcagatg tgttagtact
     4681 agacgatatc gtaaaactat atgatgagcc gttaaatgga aaaacaatcg gcgctattat
     4741 cgatccaggc caagtgaaag ctttggaaag attaggaatc gattccgatg acttgtactt
     4801 caattcagga gtgatggtga ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa
     4861 aactatccat tacctcagtg aaaatggcga tcgaatcatc tatcacgacc aagatgcttt
     4921 gaatgctgta ctttatgaag attgggaaca acttcatcca aaatggaaca tgcagacttc
     4981 gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg
     5041 aaatgaaaaa ccttcgatcg ttcactttac aggacatgat aaaccttgga atacgttgaa
     5101 agatcatccg tataccaatt tatacttaaa aaaattagca cacagtactc taacgaaagt
     5161 aggcgagatc aatgaataaa aaagaaatag cagtagtcgc aagttgtaac acaaaattcg
     5221 taccacattt agctgcatta tttgtctcag tattagataa ttgcaacccg tctaaatttg
     5281 ttcggttcta tgtgattgac gatgatatcg attttgaaag taaacagctt ttacgcttct
     5341 cagtgaaaaa cgcacgaatg aactcagatg tggaattctt gaaaatcaat aaagaattct
     5401 ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc ttattatcgt attgcgattc
     5461 cggaattgtt ccgcggcaca gaagttgaac gtatcttata tatggattgc gacatgatcg
     5521 ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg
     5581 ttgaagacgc tgggttccat caacgactgg aaaaaatgaa aatttcagct aaatcaatgc
     5641 gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa atggttagat gaaaatatta
     5701 cgcaaaaagt attagatttc atcgaacata atccagaaaa attacgattc catgaccaag
     5761 atgcattaaa tgctatctta catgaccgtt ggttaccact tcatccgcgt tggaatgcac
     5821 aaggctatat catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg
     5881 aagaaactcg caacaatcct tacattatcc atttttcagg acatgtgaaa ccgtggagca
     5941 aggattttga agggccaacg aagaaatatt acgaaaaata tgcagggatg acggctttcc
     6001 gttgtgtagc taaattccct aaatatccaa aatatgcaaa agttcaacca aaaacagatg
     6061 tagttgttaa ataatagaag accacaactg aagaggggga gtaggaatgg aaattacaat
     6121 gtctaaaaat gcagtagaga cgttaattga aaaggttggg gaaaatacaa aaatcgcact
     6181 cgcgcttatc aatgattccg atccatttct aagggacaaa ggtgcttttg caaaaggcag
     6241 cttttttcaa atcattcctt ttgtttcgga atttggagaa tatgcgacta aaatcgagca
     6301 tccattattg gacatctata catctaaatt agagcaaaat tatttcggta aacgattgaa
     6361 catggacttc aacaaacagc ttgacagctt ttcattagaa aatgaaatcg cagtactcga
     6421 ttataatatc aaattaaaga actgcttctt cagctaatag tttcgagaaa gtccaaaaag
     6481 gaggtataca ttgagatact actttgtgga tgaagaaaag aatgtaatag aaacagatga
     6541 aaaaaaatat aattggctcg tacttgatag tgcaaagccc gaagagattg agcaagcaat
     6601 cgaagaattc gagcttcctg atgatatttt cgtaggaacc gaatatccgg aagaagtttc
     6661 tcggatggag cgcctttatg agacgaaact agagcatcca ttttctttgg tagtgattaa
     6721 tctagatcag tcagataatc gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc
     6781 cgataatctc ttgattactt gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac
     6841 aaagcatgga aatcatttaa actcgtttga aaagatcatc acgtatacat tattagatat
     6901 ttacacccat tacgtgaaag aactaagaga gatgaaaaag cgaattgacg ctttggataa
     6961 agcagctcgt aaaacaacgg aaaatgaaga actgtataaa caggcagatc ttgaaagaga
     7021 catcgtatat atcgatcata ccttaagaga tcaacgggaa acaatggatc aattatggaa
     7081 ttctgaaact ttcatcaaac gtttagacga tgaacagcta ctctatgatg tccatttgag
     7141 acagcgacaa acagaaaaaa tgatcgagat ctatcgagat ttattagaga gtattggtga
     7201 tctattcaac gggatgatgg acaataatct aaatcatatc atgaaatatc tagattcagc
     7261 tgcattaatc atttccgttc cggcgttgtt cgcagggatt tggggaatga atacaggagg
     7321 attaccgggt aaaagttctg ctctaggctt tatactcgta gtctttggct ctttgatagc
     7381 agcagtagtt ttaggttacc atttataccg gaaagattat acaaattaaa gaatctattt
     7441 aaaacaggta tcaaacaaag aaatttgttt tggcaataca atttaaaaac taggaggaat
     7501 acagcaatga cagaaccaaa attaatcgac ccgcgaaact tgtaccatac ggagaaattt
     7561 cctaaacaag atcaggatac accagcttta cagcagaata tgattcctgt tcctgattgt
     7621 ggagaagaat cctatgaagg caataatcaa ctagaaaacc gccgtgtact aatcactggt
     7681 ggagattcag ggatcggtcg tgcagcagcg attgcatttg ctagagaagg ggcagatatc
     7741 gctcttcaat tcttcccagg agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa
     7801 gatgcaggac gtaagattgt cttactacct tttgatcttc gtgacgaaca agcacctaaa
     7861 gaaatcatag ataaagccgt gagtgaactt ggcgggctag atacgatggt actaaacgca
     7921 ggacagcaaa tttcttgtca gtctattgct gagttaccac ttgagcaagt caaagacaca
     7981 tttatgataa acatcatcgc tatgtttgct ttagtgaaag aagctgttcc gcatatccca
     8041 gcaggaggag caatcgtaac aacaacttct gttcaagcat tcaatccaag cgaacattta
     8101 ttagattacg cagcaacaaa agcttcaatc gctaacttta cgatcggttt agcaaaacag
     8161 cttgcaccta aaggaatccg tgtaaatggt gtagcacctg gtccaatttg gacaccatta
     8221 caactagacg aaggacaacc ttctgattcg atccctgaat ttggtcagca aagtttacta
     8281 gaacgtgcag gtcagccagg agaattagca ccagtttatg ttttcttagc atcgaataaa
     8341 gcaagttatg ttacagccca aatttacgga gtaactggtg gagaagcgat caacttataa
     8401 gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat
     8461 cggagtgatc ggaacattac ttttgctatc tgtgcttttg tttgcgatac tattaagtgc
     8521 aacctatcca ttgccttttc agctagagaa gtttcgattc ttttccatca ctaattcata
     8581 cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct attttgacaa ttatcacgtt
     8641 acttgttttc ctgttttatc caaaaacaat cggaaacttt ctactgaaac atggtgatgg
     8701 ggagttgacg ttggataaaa aagcaattga aggaatggtt cgttctcact tgcatgaaga
     8761 tgaattcatc cattcaccaa aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa
     8821 tgtaaaaggg gagttgaaac gaacttcagc actcattggt aaaacaggaa cattgatgaa
     8881 agaaatcgaa aatgaagtac aacacatatt gggaacaaaa gagccagtga aagtatcggt
     8941 gaaatacaca ggctttgaag aaccagctaa acgaagcaat gaccgcaaac gagtagaata
     9001 gggggggacc aaaaatgaaa gaactatttt cgacatacaa gctccctatc atctttgggg
     9061 tacttggatt gatattagcc gttttatgga tcaccgtggg ctttttcaag accttattga
     9121 tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta tctgaaacaa acgaaaatct
     9181 tagatcacta ttttaaatct taggaggaaa catccatgaa taacaataac aaaaataata
     9241 acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc agacccaacg acaccaaacc
     9301 aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc agtatctgca catccagatc
     9361 cagtagtccc taacgttcaa caaggagtag aagagaagaa acatgatgca gaaaagcatg
     9421 cacctcatca tccaggaact ccagaatcta aagttccaga acaacatgat gcaatcgatc
     9481 cttctaaacc tgtggaagag caaaaacatc cttctgacca tgcgaaagca caagaaaaac
     9541 ttagttctga ccacacaaac ggacaaggaa aacttggttc tgatcataca aataaccaag
     9601 gaaagcatga ttctgaacag gaagattcaa tgcgcggtga gctgaattat gaagataaag
     9661 ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga cggtttatta tctgcaaacg
     9721 gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac agataatgta acggctggga
     9781 tcgaaacaga agtcggtaaa aaacaagtag cagttgatat ggatgtcatt gtggaatatg
     9841 gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt aatcggcaaa gaagtaaaaa
     9901 atatgacaca tttagaagtg atcgaagtca acgctaacgt agtggacatc aaaacaaaag
     9961 aagaatttga aaaagatcaa gaaacggtgc aagacaaagt aactgacgca gcgaaaaaaa
    10021 ccggtgaatt tgcttcgaat caaacagata aagtaaaatc agcagctgga agcggcgcac
    10081 aaaaagtaaa agaaaataca gaaccacggg ttcagtaata aggaagtaaa acattaggag
    10141 gaacgaaaca tgggatttat ttggtcactt atcgtcggag gaattatcgg agcaattgca
    10201 ggagcaatca cgaataaagg ttcttcgatg gggattattg caaatgtcgt agcaggttta
    10261 gttggatcag cacttggtca agcattatta ggttcttggg gaccaagtat tgcaggaatg
    10321 gcaatcgtgc catcattgat tggtgccatc attttagtac taatcgtatc attctttgct
    10381 cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa agctgttctt catcaagtaa
    10441 taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttgggc atattatcga
    10501 agatgcaagc ttttgaacac tgtttatacg gaataagagg ctgtgacaag acttttgtca
    10561 cagcctctta tttatgcggg caaattccca attagatttt taaaagtttt tccttaaatg
    10621 tgtctttatt gatacagaga agcttcttta ataaaacaat gcctccctaa attttcttga
    10681 gataggaaag gtgaacatac aaaataatac gaccactttg aaaacccaga tgatccacaa
    10741 atggatcgta tgatactgat gaagtaaaaa gcaatggatc agaaactcaa agagattgaa
    10801 aaagacaagg aagaaataat atctcttttt tactcttgtc tgttttagcc aataatagct
    10861 cgtaaagtaa tctagacaaa caaatagaag taaacaaagt ttcgtaagag cagcaatcgt
    10921 atcatgttgc tctgctagac gattgacgag caggaaaaat gttggtaatg gaatatagat
    10981 cacgagtaat gaaagaaaaa agtaaacaat aaatggcata ctattcctcc taattgtgtg
    11041 agttcgccct cattttaaaa taacgatatt tgtttgtgaa gctttttcgt gtcgtaacaa
    11101 atgttatcta atgtattttt aaataaataa tcattattat caaaataatg
//
LOCUS       DAZAJZ010000096        11050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000096 DAZAJZ010000000
VERSION     DAZAJZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.097"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..568
                     /locus_tag="ACG86D_002424"
     CDS             113..568
                     /locus_tag="ACG86D_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141542.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            930..1205
                     /locus_tag="ACG86D_002425"
     CDS             930..1205
                     /locus_tag="ACG86D_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141543.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            1218..3296
                     /locus_tag="ACG86D_002426"
     CDS             1218..3296
                     /locus_tag="ACG86D_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN3141544.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            3459..5342
                     /locus_tag="ACG86D_002427"
     CDS             3459..5342
                     /locus_tag="ACG86D_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3141545.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            5354..6301
                     /locus_tag="ACG86D_002428"
     CDS             5354..6301
                     /locus_tag="ACG86D_002428"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN3141546.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKRPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            6321..6848
                     /locus_tag="ACG86D_002429"
     CDS             6321..6848
                     /locus_tag="ACG86D_002429"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN3141547.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6911..7564)
                     /locus_tag="ACG86D_002430"
     CDS             complement(6911..7564)
                     /locus_tag="ACG86D_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN3141548.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7696..8538
                     /locus_tag="ACG86D_002431"
     CDS             7696..8538
                     /locus_tag="ACG86D_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3141549.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8696..9592
                     /locus_tag="ACG86D_002432"
     CDS             8696..9592
                     /locus_tag="ACG86D_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3141550.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9595..10308
                     /locus_tag="ACG86D_002433"
     CDS             9595..10308
                     /locus_tag="ACG86D_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN3141551.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            10328..10846
                     /gene="msrA"
                     /locus_tag="ACG86D_002434"
     CDS             10328..10846
                     /gene="msrA"
                     /locus_tag="ACG86D_002434"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3141552.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10843..>11050
                     /locus_tag="ACG86D_002435"
     CDS             10843..>11050
                     /locus_tag="ACG86D_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN3141553.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYL"
BASE COUNT         3840 a         1885 c         2293 g         3032 t
ORIGIN      
        1 aagtgggcag atacgtcatt agaagaaaaa acataagatg tgaccactta cataaatttg
       61 tgctaaactg aggtagtaca agaataaatt acatcataaa ggagtctttt taatgagtaa
      121 agaaatgaca gcgctaaaat tttattttag aaatggagaa acctggacga tcaaccgccg
      181 tcatatcggc gatctttgga tcaaacaaat cacaacaagt tttggacgaa tcaatggcag
      241 tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc gaaatcttcc atgaagggga
      301 cgcagttgcg acacatgata tcaacctagg cggactggaa atgggcatgt ttaatcgcgc
      361 attgaaatac gaagatatcg agcgaatgga aatcctttac cgaaatggca cacctgatct
      421 tgtgtacttc ccatatctag ataaaggaac agaaggatta gacaatcaat atcaatcaac
      481 aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc aatccagatc aacgtgtaga
      541 agatgtatac ggggaatttt tcgaataaat cgatttttac gcaaaaatga ggctgtgaaa
      601 gagatgtgtc ttattactac tgataggctg tctcttccac accgtttatt cagaataagg
      661 gactgtgaca agatttttgt cgcagcccca tttttttgtt gtgataaaag aacaagaaag
      721 gcaggaatgg gaaagtaaac atccttttgt cagaaacttt tatctttctt aagttttact
      781 tgatatctga tactatttgg aatagaatga ggcgttgaaa aatttatgga aatttctaac
      841 tagcaataaa cgatgaaaca atgaaatatt tttcatccaa aaagaacttt gctataatga
      901 tggaagtttg tgtataggag atctggaaaa tgagagagag ttttgaacaa caaaaaaaac
      961 tgttgcatga tcgctatggc gccctttcga tggatgaccg ccgtcaaata ctttgtaagt
     1021 tgcgtaaaag aaatatattg atgtatcgtc agttggaacg tctgaaacat gatctgcttc
     1081 gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag gttgaagtag
     1141 tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa aatgcttaca caaaataaaa
     1201 aataaagggg atggacgatg gaatttgctg agttagttat tgtatttgca gtaacgatca
     1261 ccttatcgaa catagctagc cgcattttgc caatgattcc agctccactg atccaaattt
     1321 tccttggagt ggtattaggt ctgacagagt ggggacaatc aatcgatttt gaacctgaat
     1381 tgttcttagt tatgattatt gcaccattgt tatttagaga aggagaaaaa gcagatattt
     1441 cttcgattct caaaaatttc gggacaattt tgttcttggc atttggagga gtcattttga
     1501 cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca cttgctgcct
     1561 gctttgcttt tggagcagcg ttaggtccga cagatgcggt agcagtcagt tctctttctg
     1621 gacgagtgaa tatccctaaa aaggcgatgc atattttgga aggagaaggt cttttgaatg
     1681 atgcatctgg tgttactgcc ttccagtttg ctcttggtgc gctgatcacc ggatcttttt
     1741 cagcagtaaa cgcggggatg agtctagtag tttctagtat tgggggagct ttgatcggct
     1801 ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca tcagcacaag
     1861 atgtcacagg gtatctgctg atcgaacttt tgcttccttt tctagcctat gtattggcag
     1921 aggcatttga tgtatctgga atcattgctg cagtggcagc aggtattctc caagcttctg
     1981 gttttaggaa aatctctgtg tttgatgcag aattgtcgag cttgtcccac agtacatgga
     2041 caactattgc ttttacactg aatgcattag tatttatatt tttaggaatc gaattgacgc
     2101 aagtgttctc gccagtatgg ggagacggtt tatatccaaa cggtctttta ttagcgatta
     2161 tcgtattaat ctctgtcatg ctttttgtga tcagattcat ttctatcagt cttttttatg
     2221 tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg atcttaacat
     2281 ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt tattttgcca ttttcaatca
     2341 ataatatgat gttttaccag cggtctttgc ttttgtttct gacggcgggt gtcatcttag
     2401 tgactttggt catagggatc attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa
     2461 gcacggattt gaatgctttg atgattttag aagaggtcgt tgaagttttg cgaaaagaaa
     2521 taaaggaaat tgatcgtaat acaaaagaat ttttagccac tgaagcagtg atagaaaatt
     2581 atcaagaacg gatacgtgat ctgtacttgg aagatttaac cgatgatgaa aagcaagaag
     2641 tacaagaaat acaagcactg attttgtcga tcgaacggga tggattggat gaaagttatc
     2701 gttcgggcaa gctttcttct aatggttatc gtttttactc ccgttttctt tctcgattcg
     2761 aacattctat cacaagccag attttgtctt ttatcggctt ttggttcatt gttgtccgta
     2821 ggttaatgcg aatcgttctc catccgaaaa tgttttggca aagaagatat gctgatcgtc
     2881 agacatttat tagtgatgaa gatatccaag aaatcagaga gacatatcaa aaaaatactc
     2941 agttgattat tgaaagttta gataacttga ctgatattta tgatgaaacg atggttcaat
     3001 tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt
     3061 cgtggatgat ccagcaagat agtctgttta cgaaaaaaat gctaagaggt tattatcttg
     3121 aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca
     3181 gctatcgcag gaatatcaat ttgttagaat cttatacgat gaacaaacca tctgataact
     3241 tttctttccg atttgcgttt cgagcaaagt caaaaaaaac gagtccaata aaataaaaag
     3301 gtaaaaattt tcaagtttta taaaaaacaa aacatatcag aacaggatcg aatcggtcct
     3361 gttctttttt tgagaacaca aaaagaacaa ataccaaaaa aattttcctg aagttcacta
     3421 tttttatgat acaatatcca agataaggag ggaacatgtt gaaagaatta aaagtaactg
     3481 acctcaaaaa aacttatggt gaaaaagatt tgtttgacca gatttctttt ttggtccacg
     3541 aaaaagaccg tatcgggttg attgggacga acgggacggg taaaacaagt ctcttaaata
     3601 ttattgccgg tatcgatagc agcgacggtg atcggcaaac agtgttttat ccaactgatt
     3661 accgtattgg ttatctttct caagcagctg aattttccga tgaattaact gttctccaag
     3721 cagtctttca aggaaacagc cccttgatcc aaactgttcg agcatatgag gaagcattga
     3781 tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa acgttatgct aaagcagaag
     3841 aacaaatgaa taaagaagat gcttggacta cagataccaa cgcaaagatt attttgcaaa
     3901 aattaggaat cagtgaatta gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac
     3961 gagttagtct ggcgcaagtg ttgattgatg aacccgattt actattacta gacgaaccaa
     4021 caaaccattt agattatgaa gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg
     4081 gcgcactatt gatggttacc cacgatcgtt attttctaga tagagtagca aaccgaatct
     4141 tcgaactttc atttggaaaa ttatacgagt ataaaggaaa ttatgaagct tacgtcttag
     4201 aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga aaagcgcaag cggctttata
     4261 aacaggaatt agcttggatg agggctggcg tacaggcacg tggcaccaag cagcaagcac
     4321 ggatcaaccg tttcgaagat ctaaaggaga acttgtacca agtaaaccaa gaagatgatt
     4381 tagagctgaa cctagcgaca caacgtttag ggaaaaaagt actggaaatc aaagatggca
     4441 gttaccgtat aaatgaccaa acactgctag aacatttaga tttattgatt caatctcggg
     4501 aaagactagg tattactggt aaaaatggtg cgggaaaatc gacattgctt aatattctag
     4561 ctggcagaat tcccctggat agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt
     4621 attatacaca agaaaatgaa gaaatggcac cagataaacg gatgattgct tacttacaag
     4681 aagctgctga agaagcaaaa acagcagatg gcagtcaaat cggagtagca gaattacttg
     4741 aacgattttt atttccaaga tttatgcatg ggacactgat aggcaaactt tcaggcggag
     4801 aaaaacgccg gttatttttg ttgaaactgc tgattcagca acctaatgtt ttattattgg
     4861 atgaaccgac caatgatttg gacatagcaa cattgacgat tttggaagat tattttagaa
     4921 gttttccagg tgctgtgatc actgtttccc atgaccgtta tttcttggac aaggtagcag
     4981 ataagttatt agtattccaa ggaaacggaa aacaagaact ttattacgga aacatgtcca
     5041 gttatttact gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa ccaaaaatac
     5101 agagcaagga acctgctgaa aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa
     5161 cgattgaaga tgagattgca gaacttgaag aaaaaatctc attgctccaa gaagagatga
     5221 atcatcaagg agataatttc actcgattac aagaattgca aaacgacgta tctgaaacag
     5281 aagcacaact tgaagaaaaa atggcacggt gggaatactt gagtgaatgg gtagaagatt
     5341 aggaggaaaa aggatggaac aagcgtatct tgacttagga agaaaattgt tggaagaagg
     5401 gcatcgtaaa gaagacagaa caggcacagg cacattaagc ttattcggat accaaatgcg
     5461 gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt ttggattgat
     5521 caaaagcgaa ttgctttggt ttttaaaagg agatacgaat atccgctatc tattacaaaa
     5581 taaaaaccat atttgggatg aatgggcatt tgaacgctac atcaaaaggc cagactataa
     5641 tggaccagat atgactgatt ttggccatag atgtttgaca gatcctgaat tcaatgaaga
     5701 atataaaaag cagagcaagc ttttttgcga gaagatccta accgatgatt cctttgctga
     5761 aacatacggc gatcttggac acatctacgg ctatcaatgg cgtcactggg aaacaaaaga
     5821 cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc
     5881 tagaagattg atcgtttctg catggaatcc ggaagatgtt cctagcatgg ctttgcctcc
     5941 ttgtcatacg atgtttcaat tttatgttca agagggacgg ctaagctgcc aattgtatca
     6001 acgcagtggg gatgtgtttc taggcgttcc gttcaacatt gcaagttatg cgttgctgac
     6061 tcacctaatt gcacatgaaa caggtttagc ggttggcgaa tttgttcaca cattaggaga
     6121 tgctcatctt tatatcaatc atatcgaaca aatgaaagaa caattgagcc gagaaatacg
     6181 cgcatttccg actttagttt tgaatcagga aaagcagtca gtatttgatt ttgagatgga
     6241 agatatccaa ttagaaggat atgacccaca tccggcgatc aaagctccta tcgctgttta
     6301 gaatcagaaa ggatgagaaa atgttagctg caatttgggc acaggatgaa aacggattga
     6361 ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa
     6421 tgacagaagc aaatacacta gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc
     6481 cgttgccgaa tcgtcagacg attgttttga cgagagattc ttcctataaa gcagaaggcg
     6541 tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca gacggtatct
     6601 ttttcatcgc tggagggtca gctgtttatc aagaatttct gccattttgt acgatattat
     6661 accgtacagt catccatcat gtattcgatg gagatgctta ttttccacct gttgactggt
     6721 ctgattggtc actgatcaat actagtcaag gagaaatgga tgaaaaaaat cgttatccgc
     6781 atcaatttga aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga
     6841 cgatataaaa aagctatgga tagactagct gttatttaga gctgtcccat ccatagcttt
     6901 atttttttta ttaagtaaag aataaaacag agaagtacat aaaacctgca cctaacataa
     6961 caaacaaatg ccaaacaaca tgcatatagc ggatagaatt caagctataa aagaatgcgc
     7021 ctatcgtgta agaaagcccg cctgctacca tcaaaataac accagtcatt ccaagagagt
     7081 gataaagagg aacagcagcc acgacacaga gccaccccat aaaaatatag atgatcgtag
     7141 atacttttga tactctctcc cgtttatgca acgtcaacgc tttatatata atgccaagaa
     7201 tcgcaaacaa ccagattaat acaaataaag tccaacctag ccatcctcgg atactcagca
     7261 aacaaaaagg agtataactt cctgcaatca aaagaaaaat cgaactatga tcgaatactt
     7321 ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa caaagtagac gttaaaaata
     7381 aaagaatcat catcgatcca tagatagcat aagagacaat atggagggag gaatgcaagt
     7441 gggcaccttt tactaaaaga accactagtc ccgcgatact caatccagtc ccaattccat
     7501 gggtgactgc attaaacact tcatttaata tttcatacgt tcgattaaat ttcgtttttt
     7561 ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa atcattattt gtctgttata
     7621 ctaaaaatag aaattgtctt acatgtagtt taacatgaat aaggcatctt gttaaagaaa
     7681 gagagagaga aaataatgac aaatgtaaaa atcgtaacag attcttcatg tacgatggaa
     7741 aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc tatccatcat gatcgacgga
     7801 acagtatatc ccgatgatga ccaactagac ggcgaaagat ttatggaaat gatggcgaaa
     7861 gcaaaaaatc tgcccaaaac aagccaacca ccaattggag aattcatctc gatgtatgat
     7921 gagctaggag aagacggcag tgaagtgatt tcgatccata tgacaaaagg cttgagtgga
     7981 acagtagaag cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac
     8041 agtgatacca ctgaccaagg attatctttt caggtaattc gagcagctga attagcaaaa
     8101 gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa
     8161 ttatacatcg gaatatccac actagataac ttggtaaaag gtggaagaat cagccgaaca
     8221 actggtttgc tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc ccattctgaa
     8281 ttaatccctg taactaaggg gcgaggaaag aaaacgttcg ataaatggtt taataatttg
     8341 aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca
     8401 aaagaattgg cgcaagaatt caaagagggt ttgcaagaat tattcccgga catgcatatc
     8461 cctgtactgc atacgaatcc ggttatcgct acgcatactg gtaaagacgc gtttgcaatc
     8521 atgtattatg tagattagtc gaaaagagga taagccctca gcctgaaagg cttgcagctt
     8581 attctctttt tctttataaa attaggaaaa taattgtttt cttttttcat aaagactacc
     8641 tttttgataa aatttttgat aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa
     8701 attaacgaaa cgacatttga tcctcgtttt gttcccatta ttaattgcga tcatcatcta
     8761 tgcaggttta gctgcttcta taccgaaagc tggcccacta atgaaaccag taactactgt
     8821 gagagaagat ccaaataaaa aagaaaaact tcactacata gctatcggag attcgctgac
     8881 tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg caaatgatct
     8941 aaaagaccgt tataatttga ccgcggttga aacagaaaac tatggtgtaa atggtgaacg
     9001 aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac
     9061 agctgatttt atcacactga ctgttggcgg taatgatttg atgaaagtta tccaaaataa
     9121 tttattcggg ttaacggtca attcctttaa aaagccactg aaaaactatc aggaaaatgt
     9181 cacagaacta ttggaagaat ttcgatccct caataaaacc gcacctattt acattttagg
     9241 catctacaat ccattttatt tgaactttcc tgagatcact gacatgcaga caattgttaa
     9301 caattggaac aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct actttatccc
     9361 aatcaatgat ttgctttatc aaggattgaa tcaggaagta ggtgtcacag aagaaggaac
     9421 agatcaaaca actagcacga cagaaaatct aaatggcatc aaaaataatg tgctctatga
     9481 agaagatcat ttccatccaa ataatttagg ttatcaattg atggcaaatg cagtgagaga
     9541 tcagctgatc gagacacaaa accaatggtt agagaaggag gatgatcaat aaacatgaaa
     9601 acaagatctg acgtatcttc aaacaaaaca aagaaaacaa attactggaa attcgccttc
     9661 cttatccttc ttggggtagt acttggttcg accgtatttt taggcagccg tatttttgct
     9721 aatcgggaac cagaactccc agaaatacca gctcttacgg aaagacaagg agatcctgtc
     9781 ttaacgatca attcaaataa agaaaaagtc aatcaaatca tcagcttttt ccttagtgag
     9841 tatcaaaaag atagtgatat tgaatataaa ttttatttgg aaaatgaagc actactcaat
     9901 ggtactttcg aagtactagg ttttcctatc gatttctatt tatattttga tccgtatgtg
     9961 atggaaaacg gaaatgtcca actaaaagct agaagtcttt ctatcggaac attgagtttg
    10021 ccaatcaaag atgtgatgaa tatgatcaag agaaactata aattaccaga ttggatcgaa
    10081 gtcaatacag atgatctgac tatcatgctt aggttggatc aattccgaat gcaaaacggg
    10141 atgtatatta aagcagataa aatcgactta gtaaatgatg acattcggtt tagtctatat
    10201 ttgccaaaag atacctctga tgcttcaaac aactcaaata ccaacaaaac tagcaccagc
    10261 agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg atgagtagta ttagaggggg
    10321 aatcataatg gaacgagcaa tatttgcagg aggctgcttc tggtgtatga tccagccttt
    10381 cgacacgctt cctggtatcc atacagtgat gtcaggatat actggagggc atgtaccaga
    10441 tccgacttat gaacaagtaa aaagccaaac gactggacat acagaagcag tggagattat
    10501 ctatgatcca acattgattt catatgaaca attagtagaa ctttactggc agcaaactga
    10561 tccaactgac gctttcggac aattcgagga tcgcggtgac aattatcgcc cggtgatctt
    10621 ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag
    10681 tggacgtttc gaacagccaa tcgttacaaa aatcgaacca gcccaaccat tttatctagc
    10741 agaagaagac catcaagagt attataaaaa gcatcctgaa aattttaaaa gaaaccatgc
    10801 acgccgggct gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg aagctttttt
    10861 cattatttaa tgacactgcg aggcggtggc acacatgatg ctttatctgt tttcgctaca
    10921 gaagccggta aagacatcca atttcctaag cacagctcta gctacgaaga aatttctaat
    10981 tatctggaac tgaatgtcga ctatttgccg tccatggata tctttgacga agcatgggaa
    11041 aaatatttag
//
LOCUS       DAZAJZ010000097        10697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000097 DAZAJZ010000000
VERSION     DAZAJZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.098"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(215..1063)
                     /locus_tag="ACG86D_002436"
     CDS             complement(215..1063)
                     /locus_tag="ACG86D_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3141554.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1227..2129
                     /gene="gnd"
                     /locus_tag="ACG86D_002437"
     CDS             1227..2129
                     /gene="gnd"
                     /locus_tag="ACG86D_002437"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN3141555.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2131..3132
                     /locus_tag="ACG86D_002438"
     CDS             2131..3132
                     /locus_tag="ACG86D_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN3141556.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3129..3413
                     /locus_tag="ACG86D_002439"
     CDS             3129..3413
                     /locus_tag="ACG86D_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN3141557.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3446..4243
                     /locus_tag="ACG86D_002440"
     CDS             3446..4243
                     /locus_tag="ACG86D_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3141558.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4257..5090
                     /locus_tag="ACG86D_002441"
     CDS             4257..5090
                     /locus_tag="ACG86D_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3141559.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5108..5965
                     /locus_tag="ACG86D_002442"
     CDS             5108..5965
                     /locus_tag="ACG86D_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3141560.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5987..6496
                     /locus_tag="ACG86D_002443"
     CDS             5987..6496
                     /locus_tag="ACG86D_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN3141561.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6533..6934
                     /locus_tag="ACG86D_002444"
     CDS             6533..6934
                     /locus_tag="ACG86D_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141562.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7046..7399
                     /locus_tag="ACG86D_002445"
                     /pseudo
     CDS             7046..7399
                     /locus_tag="ACG86D_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7433..7876
                     /locus_tag="ACG86D_002446"
     CDS             7433..7876
                     /locus_tag="ACG86D_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3141563.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7893..8978
                     /gene="uxuA"
                     /locus_tag="ACG86D_002447"
     CDS             7893..8978
                     /gene="uxuA"
                     /locus_tag="ACG86D_002447"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3141564.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8971..9684
                     /locus_tag="ACG86D_002448"
     CDS             8971..9684
                     /locus_tag="ACG86D_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3141565.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9891..10040)
                     /locus_tag="ACG86D_002449"
     CDS             complement(9891..10040)
                     /locus_tag="ACG86D_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141566.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10436..10636
                     /locus_tag="ACG86D_002450"
     CDS             10436..10636
                     /locus_tag="ACG86D_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3141567.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3569 a         1694 c         2315 g         3119 t
ORIGIN      
        1 aattttttac tactgtatac taactttgtt atcgcatcta acaagtaact cgaataatac
       61 caatacaacc ttctccaaag atgcgatgac atacgttcta acccaccgac tattgatgtt
      121 ctgtaccacc cttgcagaac caaaaaagcc actaccaaat atttggtagt ggcttttttt
      181 gttttatgga tttttcgctt tttctgttat ggtgctattc tttgatttca gaaatagtat
      241 gtcgaacttt agcgatagaa cgacgtgatt ctttttcgtt gtaaaacatc aaaatcacat
      301 atagagagtc caaaatgctt agttgagaaa tacgggaagc taaagcttca gatcggaatt
      361 ctgtttcttc agaaatagaa ataaaaacga catcgccaag tttagcaagg gaagaattag
      421 cttggctagt aataacgatt gtttttgctc cagtttttct tacggtttca gcaatatgga
      481 ttgtttcttt tgattgccct gtgtgagaaa tcaaaacagc acagtctttt tccgtcaata
      541 aagaagcttc catcaattga atatggtaat ccgtgctgtg agaagttgcg ataggggaac
      601 gcaaaaattt atgataagcg tcagttgcta caatctctga accgccaaca ccgaaaaaat
      661 agacacgttt agattcattg ataatagtag ccgctttttt tagatccttt tgttgtaaca
      721 acttttttgt gtcatttaaa gttgaaatac tagactcgaa caccttttgc gccatagtga
      781 gctcagtatc tgttttctga atattttcgt ggatagaaat agcagataga tcattttctt
      841 ggatcaacat ggccatttta aaatctttaa atccattata accaagtttt tttgtaaatt
      901 gaaaaaaagt agagtcagca atgcccagtt cacttgctaa attgctaata ggactatgac
      961 ttactttgct tggattttct aaaatataat cggcgatagc ctgttctttt gggctgaagt
     1021 cgttgtacat tgttttgatt tgtagtctta tggagacgct catgagaatt cctcattttc
     1081 ataataagat tatcattttt tccgttctca ttaatgttaa agatacaagg agagtaggaa
     1141 aaaatcaaac aaaaaatatt tttgtaaaat ctatttacaa aaaaattttt ttgtataatg
     1201 agggtataag aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg
     1261 gattgaattt agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt
     1321 caagtacagc tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg
     1381 aatttctagc tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga
     1441 taaccgaaaa catgttaaga aaactttttc caaaactcgg accaggagac attattattg
     1501 aaggcggaaa ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg
     1561 aaatcggtta cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct
     1621 gcttaatgat tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg
     1681 tagcaattga aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa
     1741 tgatccataa tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg
     1801 tacaaaaaag tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt
     1861 cggtgattcg cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt
     1921 taagtaatta tcggggaatc gtttctgcat caggtgaagc aaaatggacg gttgaaacag
     1981 cgctagaaat ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt
     2041 cacaggaaga agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg
     2101 gacatgaaat agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat
     2161 tccagcagtt tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac
     2221 gttcatttct ttactgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa
     2281 ggattcaata tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac
     2341 aaagatgaac tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa
     2401 gcagcaaaag aacaccagac aatcgtatct atcatacctg ctttaattga acgaaatgct
     2461 gtagcagaaa ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa
     2521 gcaagagtca aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt
     2581 aacaaaacag ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg
     2641 cgtaacggtt ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc
     2701 gaaagctttg gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt
     2761 tgtttatgtg cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat
     2821 cagatgaaag aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa
     2881 gcgatgatta atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa
     2941 gaagaaccgg gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca
     3001 catatatcgg cctacacaag ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat
     3061 gtcgaacaga tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat
     3121 ataaaaggat gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt
     3181 atttatatga agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga
     3241 aaagagtact ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat
     3301 ttttgaatga cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg
     3361 ctgaagccag acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt
     3421 tggcggagga aaactgaccg atctagtggg ttatagtgcc aatcttgcaa gaatcgattt
     3481 cggagtcatc cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta
     3541 taaggagaat ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt
     3601 tataacagat ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc
     3661 agatacacta gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga
     3721 tgaatcattt ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa
     3781 tcattctgct aaagcaatcc aagatatggc acaaagaaaa ctgacatcgg agttcaaaca
     3841 gctttcagag attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc
     3901 aagaaatgcg gcagcacatg ctatgcatga cgctatcagt aaatatattc cggaaagcca
     3961 tcgttttcta catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg
     4021 tcgctgggaa aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc
     4081 tctagaacaa atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt
     4141 tattgattcg aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat
     4201 acagtcaatg atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg
     4261 aaacattaag tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc
     4321 gctggttagg tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag
     4381 tcgtcgggtt gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa
     4441 tgatttatat gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag
     4501 cagctatcgc agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg
     4561 ttgcggtgcc agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata
     4621 cttttttagg taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc
     4681 gatcaattat tctttatcca acggttgcct catttttatt attcgttccg cttgttttca
     4741 ttgctttata tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag
     4801 tcgttatcca cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa
     4861 ctgtatacgt tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga
     4921 gtgttgtttt gaaatcttta gatattacga tggttactta tgccattttt gggattatta
     4981 tcgctttgat ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata
     5041 acaattcagc atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga
     5101 atgggaaatg gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa
     5161 agatgtaaca aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga
     5221 acgttacttg gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa
     5281 agatgaagac tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt
     5341 aagctggggt ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga
     5401 agaagagtat gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc
     5461 gggattgatg ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata
     5521 tatttttatc gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt
     5581 tccttttatc tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc
     5641 tttcactatg ggccgtaatg ctacaggcct aatgcaaaac actggtgttc agacagcgat
     5701 tgaaatgcta tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa
     5761 agtagaatct tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct
     5821 tgatcaaatc gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta
     5881 tacgaaaaaa ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt
     5941 gctagcagga atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa
     6001 tttagcgcgt gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa
     6061 aagtggagtg aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag
     6121 agatctttac aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga
     6181 gaaagctaaa ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc
     6241 aaaaacagtc caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa
     6301 tattggaggg attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta
     6361 tttaaataaa tcagatgctg aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa
     6421 tatttatttt caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa
     6481 gtttgatttg tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa
     6541 gaacgagctg tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac
     6601 aaaaaagagt aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac
     6661 agatgtgaca gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg
     6721 agatatcgaa aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt
     6781 aggaagttat ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt
     6841 attgtccttc attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa
     6901 tcctcatcac gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg
     6961 gagctacttt ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat
     7021 tcgaaatact tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag
     7081 tgtagcgtat gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag
     7141 atcgggtatg aaaaaagagg ctattctttt ttatctagac tctgttgggg cagatacacc
     7201 gctacagttt tcgggaaatt atttttcaaa taaagaacag tgactgaaac aagttgataa
     7261 gttgaaagaa aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt
     7321 ctatttgacc aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat
     7381 tattgctctt tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga
     7441 aatcatgatc gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat
     7501 tgtcgggaca tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac
     7561 attcggagag aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt
     7621 atttgttgat ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact
     7681 gactgaagaa aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt
     7741 agaagcaatc aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt
     7801 caaacaagga aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga
     7861 tgaggacgat ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg
     7921 aaaaagggga ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa
     7981 ttggaacttt gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt
     8041 tgaaagcatc tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc
     8101 acgatgcaat caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa
     8161 caatcaaaaa cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt
     8221 ttggatgggc gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat
     8281 acgatcagaa aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc
     8341 agtcaaaggg attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc
     8401 tggaaaaact gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt
     8461 tagaaaaagt gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg
     8521 atccgccatg ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga
     8581 aaattctaaa tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag
     8641 gggcaaatcc ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt
     8701 ttgtccactt cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc
     8761 cctctacaga aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt
     8821 tcgacggtcc tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg
     8881 gatatggcct gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga
     8941 taagtaagga aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat
     9001 atggatggcc ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca
     9061 caaaagtatg attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa
     9121 atggcgataa gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg
     9181 cgagaagcaa tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg
     9241 aaaaaaggag cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg
     9301 gcttcgtcta atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc
     9361 ttgtttgatc aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc
     9421 tttttgaaag cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac
     9481 tcgattgcag ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat
     9541 catttgatag ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta
     9601 tatgaatcca ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta
     9661 gaaagtaaag ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg
     9721 tcctgcacga ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc
     9781 ttatttgtag tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag
     9841 atgcgtttaa agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat
     9901 ttttttaatt cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt
     9961 tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata
    10021 tctttgttat agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg
    10081 aacgcgttga taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt
    10141 taagaaattt taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg
    10201 aatcaatcgc ggttattcaa ctttttcttc gattcgaaga caatagaaga caatgaattt
    10261 tatatttcat tgcattatcc ttgtgttatt agaaaaagca tgatatatta aatgagtaat
    10321 ctattttgaa acgtaatttg agcgatatat ctacatagat aaaactcctt ttgcctatca
    10381 aatatttatt gcaacgaaaa aacgtacatt gatacgtatc aggaggaatt tatttatgaa
    10441 taacggtaca gtaaaatggt tcaacgcaga caaaggtttt ggctttatca ctggtgaaga
    10501 tggaaatgat gtatttgcac atttttcagc tatccaaggt gacggcttca aaactttaga
    10561 agaaggccaa gcagtgtctt acgatgttga agatggtcaa cgtggccctc aagcaacaaa
    10621 cattgttaaa gcttaataaa aattgacttt taaccgtctt ttcgaaagaa aagacggttt
    10681 ttttgtacga aaaaatt
//
LOCUS       DAZAJZ010000098        10212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000098 DAZAJZ010000000
VERSION     DAZAJZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.099"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG86D_002451"
     tRNA            8..81
                     /locus_tag="ACG86D_002451"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG86D_002452"
     tRNA            101..173
                     /locus_tag="ACG86D_002452"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG86D_002453"
     tRNA            201..274
                     /locus_tag="ACG86D_002453"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG86D_002454"
     tRNA            288..361
                     /locus_tag="ACG86D_002454"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG86D_002455"
     tRNA            385..474
                     /locus_tag="ACG86D_002455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG86D_002456"
     tRNA            487..560
                     /locus_tag="ACG86D_002456"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG86D_002457"
     tRNA            579..654
                     /locus_tag="ACG86D_002457"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG86D_002458"
     tRNA            656..726
                     /locus_tag="ACG86D_002458"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACG86D_002459"
     tRNA            740..813
                     /locus_tag="ACG86D_002459"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACG86D_002460"
     tRNA            838..925
                     /locus_tag="ACG86D_002460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACG86D_002461"
     tRNA            947..1018
                     /locus_tag="ACG86D_002461"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACG86D_002462"
     tRNA            1041..1130
                     /locus_tag="ACG86D_002462"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACG86D_002463"
     tRNA            1143..1216
                     /locus_tag="ACG86D_002463"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACG86D_002464"
     tRNA            1235..1307
                     /locus_tag="ACG86D_002464"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACG86D_002465"
     tRNA            1311..1383
                     /locus_tag="ACG86D_002465"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACG86D_002466"
     tRNA            1388..1458
                     /locus_tag="ACG86D_002466"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACG86D_002467"
     tRNA            1471..1544
                     /locus_tag="ACG86D_002467"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACG86D_002468"
     tRNA            1556..1627
                     /locus_tag="ACG86D_002468"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACG86D_002469"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACG86D_002469"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN3141568.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACG86D_002470"
     CDS             3216..4688
                     /locus_tag="ACG86D_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN3141569.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACG86D_002471"
     CDS             4771..5559
                     /locus_tag="ACG86D_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3141570.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACG86D_002472"
     CDS             complement(5626..6294)
                     /locus_tag="ACG86D_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN3141571.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACG86D_002473"
     CDS             6409..8121
                     /locus_tag="ACG86D_002473"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141572.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="ACG86D_002474"
     CDS             8124..9896
                     /locus_tag="ACG86D_002474"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141573.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9978..>10212)
                     /locus_tag="ACG86D_002475"
     CDS             complement(9978..>10212)
                     /locus_tag="ACG86D_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141574.1"
                     /translation="YNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDR
                     VGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2971 a         1811 c         2320 g         3110 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag ttgattttgc
    10021 atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc atgtcctaca
    10081 cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat gtgggaatgc
    10141 cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac tgctcttaaa
    10201 gattgattat at
//
LOCUS       DAZAJZ010000099         9938 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000099 DAZAJZ010000000
VERSION     DAZAJZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.102"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACG86D_002476"
     CDS             32..388
                     /locus_tag="ACG86D_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFN3141575.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACG86D_002477"
     CDS             404..754
                     /locus_tag="ACG86D_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFN3141576.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACG86D_002478"
     CDS             783..1562
                     /locus_tag="ACG86D_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFN3141577.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACG86D_002479"
     CDS             1573..2235
                     /locus_tag="ACG86D_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFN3141578.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACG86D_002480"
     CDS             2254..3360
                     /locus_tag="ACG86D_002480"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFN3141579.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACG86D_002481"
     CDS             3350..4441
                     /locus_tag="ACG86D_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFN3141580.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="ACG86D_002482"
     CDS             4454..5194
                     /locus_tag="ACG86D_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFN3141581.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="ACG86D_002483"
     CDS             5482..7422
                     /locus_tag="ACG86D_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN3141582.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="ACG86D_002484"
     CDS             7509..9431
                     /locus_tag="ACG86D_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3141583.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9711..>9938)
                     /locus_tag="ACG86D_002485"
     CDS             complement(9711..>9938)
                     /locus_tag="ACG86D_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFN3141584.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3244 a         1649 c         2207 g         2838 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttatattt ataataattc tatctattta
     9481 tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg ccaaagttgt ggcacatcct
     9541 tttattttga gtttcttata attttttata gttgcttacg atgtcttgac tactggtaaa
     9601 cggtgttcaa gagacaattt gtcggcaata aggcacaatt ttgtaaaata tggaaagcta
     9661 tttttttaac attgtttctt agtgcttgaa aaatcatttt gaatacttct ttatacgatc
     9721 gatgtacgtt gatttaagtc taaaactttt cttttgattc cttctctgac ttttctgaat
     9781 tccgttaata tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga
     9841 aggtgttcgt gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaagtgta
     9901 atgatcaagt ctgcctgagt aaagtaatct ctatcaat
//
LOCUS       DAZAJZ010000100         9751 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000100 DAZAJZ010000000
VERSION     DAZAJZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.103"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            326..1375
                     /gene="trpX"
                     /locus_tag="ACG86D_002486"
     CDS             326..1375
                     /gene="trpX"
                     /locus_tag="ACG86D_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3141585.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1372..2253
                     /locus_tag="ACG86D_002487"
     CDS             1372..2253
                     /locus_tag="ACG86D_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3141586.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2256..3026
                     /locus_tag="ACG86D_002488"
     CDS             2256..3026
                     /locus_tag="ACG86D_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141587.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3305..4159
                     /gene="aroE"
                     /locus_tag="ACG86D_002489"
     CDS             3305..4159
                     /gene="aroE"
                     /locus_tag="ACG86D_002489"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN3141588.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4337..5500
                     /locus_tag="ACG86D_002490"
     CDS             4337..5500
                     /locus_tag="ACG86D_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3141589.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5617..6027
                     /locus_tag="ACG86D_002491"
     CDS             5617..6027
                     /locus_tag="ACG86D_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3141590.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6201..8429
                     /gene="topB"
                     /locus_tag="ACG86D_002492"
     CDS             6201..8429
                     /gene="topB"
                     /locus_tag="ACG86D_002492"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3141591.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8600..8884
                     /locus_tag="ACG86D_002493"
     CDS             8600..8884
                     /locus_tag="ACG86D_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141592.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9044..9280
                     /locus_tag="ACG86D_002494"
     CDS             9044..9280
                     /locus_tag="ACG86D_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3141593.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9277..9675
                     /locus_tag="ACG86D_002495"
     CDS             9277..9675
                     /locus_tag="ACG86D_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN3141594.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3384 a         1653 c         2070 g         2644 t
ORIGIN      
        1 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
       61 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      121 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      181 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
      241 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
      301 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
      361 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
      421 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
      481 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
      541 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
      601 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
      661 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
      721 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
      781 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
      841 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
      901 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
      961 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1021 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1081 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1141 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1201 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     1261 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     1321 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     1381 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     1441 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     1501 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     1561 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     1621 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     1681 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     1741 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     1801 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     1861 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     1921 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     1981 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2041 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2101 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2161 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     2221 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     2281 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     2341 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     2401 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     2461 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     2521 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     2581 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     2641 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     2701 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     2761 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     2821 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     2881 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     2941 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3001 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3061 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3121 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3181 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     3241 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     3301 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     3361 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     3421 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     3481 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     3541 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     3601 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     3661 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     3721 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     3781 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     3841 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     3901 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     3961 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4021 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4081 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4141 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4201 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     4261 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     4321 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     4381 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     4441 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     4501 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     4561 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     4621 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     4681 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     4741 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     4801 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     4861 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     4921 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     4981 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5041 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5101 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5161 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     5221 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     5281 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     5341 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     5401 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     5461 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     5521 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     5581 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     5641 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     5701 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     5761 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     5821 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     5881 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     5941 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6001 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6061 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6121 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6181 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     6241 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     6301 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     6361 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     6421 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     6481 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     6541 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     6601 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     6661 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     6721 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     6781 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     6841 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     6901 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     6961 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7021 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7081 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7141 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7201 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     7261 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     7321 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     7381 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     7441 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     7501 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     7561 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     7621 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     7681 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     7741 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     7801 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     7861 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     7921 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     7981 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8041 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8101 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8161 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     8221 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     8281 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     8341 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     8401 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     8461 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     8521 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     8581 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     8641 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     8701 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     8761 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     8821 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     8881 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     8941 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9001 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9061 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9121 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9181 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
     9241 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
     9301 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
     9361 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
     9421 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
     9481 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
     9541 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
     9601 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
     9661 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
     9721 ggaatatgct gattttttta tgtttaatca t
//
LOCUS       DAZAJZ010000101         9602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000101 DAZAJZ010000000
VERSION     DAZAJZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.104"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG86D_002496"
     CDS             complement(<1..613)
                     /locus_tag="ACG86D_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFN3141595.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            814..2028
                     /locus_tag="ACG86D_002497"
                     /pseudo
     CDS             814..2028
                     /locus_tag="ACG86D_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2207..2542)
                     /locus_tag="ACG86D_002498"
     CDS             complement(2207..2542)
                     /locus_tag="ACG86D_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3141596.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2526..2789)
                     /locus_tag="ACG86D_002499"
     CDS             complement(2526..2789)
                     /locus_tag="ACG86D_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN3141597.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3249..4052)
                     /locus_tag="ACG86D_002500"
     CDS             complement(3249..4052)
                     /locus_tag="ACG86D_002500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3141598.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4030..5154)
                     /locus_tag="ACG86D_002501"
     CDS             complement(4030..5154)
                     /locus_tag="ACG86D_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141599.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5170..5985)
                     /locus_tag="ACG86D_002502"
     CDS             complement(5170..5985)
                     /locus_tag="ACG86D_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3141600.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5975..6847)
                     /locus_tag="ACG86D_002503"
     CDS             complement(5975..6847)
                     /locus_tag="ACG86D_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3141601.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6932..8221)
                     /locus_tag="ACG86D_002504"
     CDS             complement(6932..8221)
                     /locus_tag="ACG86D_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3141602.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8365..8877)
                     /locus_tag="ACG86D_002505"
     CDS             complement(8365..8877)
                     /locus_tag="ACG86D_002505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141603.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8944..9093)
                     /locus_tag="ACG86D_002506"
     CDS             complement(8944..9093)
                     /locus_tag="ACG86D_002506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN3141604.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
BASE COUNT         2967 a         2027 c         1554 g         3054 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct
      841 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      901 gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac
      961 aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt
     1021 acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt
     1081 cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt
     1141 tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca
     1201 aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct
     1261 tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct
     1321 gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca
     1381 gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt
     1441 gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag
     1501 gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt
     1561 caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac
     1621 cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa
     1681 gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt
     1741 tttcatgaat tagtcgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1801 gcagaacttc ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa ccttctgacc
     1861 tatgaagaag gtatcaccag cgcaatgatc tatccttatt ccaatggaaa aattgaagcg
     1921 aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaatc attcgagaac
     1981 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
     2041 accagaatgt ggttcattct gacccggatt tgattttgca caactcatca gtccttattg
     2101 acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta
     2161 caacccatca gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca
     2221 tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga
     2281 cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt
     2341 ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt
     2401 tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa
     2461 tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta
     2521 tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt
     2581 tggacaggca atttttccgc taatctttga atattatgga ttcgtttact tttttccgac
     2641 aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg
     2701 gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt
     2761 tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat
     2821 atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt
     2881 gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt
     2941 ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa
     3001 gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat
     3061 gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc
     3121 tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc
     3181 aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc
     3241 aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt
     3301 ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat
     3361 cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat
     3421 tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg gatatgtcct
     3481 acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca
     3541 gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt
     3601 tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct
     3661 tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta
     3721 caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg
     3781 cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac
     3841 attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat
     3901 agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt
     3961 ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta
     4021 aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata
     4081 atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc
     4141 tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat
     4201 ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt
     4261 cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc
     4321 taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa
     4381 aaattgactg gctaccttta atgttccttg atcataagta actggcataa tattcatgcc
     4441 tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg
     4501 cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat
     4561 cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt
     4621 taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc
     4681 taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg
     4741 ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa
     4801 agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa
     4861 aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc
     4921 gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc
     4981 actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga
     5041 tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat
     5101 ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg
     5161 tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat
     5221 tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa
     5281 atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc
     5341 tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca
     5401 aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt
     5461 tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat
     5521 tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca
     5581 agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt
     5641 aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg
     5701 gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat
     5761 tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata
     5821 gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc
     5881 tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat
     5941 gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt
     6001 ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa
     6061 ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca
     6121 cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat
     6181 actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag
     6241 gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga
     6301 tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa
     6361 ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca
     6421 taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta
     6481 atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata
     6541 atggtttata caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat
     6601 atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca
     6661 ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt
     6721 tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat
     6781 aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc
     6841 gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt
     6901 tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt
     6961 gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca
     7021 acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct
     7081 tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc
     7141 cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt
     7201 tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct
     7261 tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc
     7321 cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt
     7381 ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac
     7441 cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat
     7501 aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc
     7561 cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc
     7621 catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt
     7681 ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg
     7741 gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga
     7801 tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta
     7861 aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca
     7921 cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc
     7981 ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt
     8041 atttatctac taattcattc actgtttttc cacctagggt atccgcatta acatgccaat
     8101 actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag
     8161 ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca
     8221 taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa
     8281 ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg
     8341 gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat
     8401 cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc
     8461 tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc
     8521 aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg
     8581 ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac
     8641 gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag
     8701 ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc
     8761 ccaagataat tgtacatagc cactaagaac aatttgtggc cagatttgat aggtttgtgc
     8821 cagcgaaaaa acggccattt ccaatccctc ttcttttaaa tcacagttaa tggtcatggt
     8881 tgccttctca ttggccactg cttgaaacac ctgctcaagg gttaccgaat ctgggacaac
     8941 ttcttacctg tcatgggcta gtattagcgt accatctgtt tgctgacgca catctacttc
     9001 aaaacaaagc tggcgatcca aacaaaagcg aagaaatgcc cagctattct cctccgtatt
     9061 gtcactacca gagtgtgcag tgatcatggt cattttgacc cctcctttaa ttccgtgatt
     9121 ttctcccaca cgtccactat accgatgaag cgttaatttc ttgttaattt cttgtaaatt
     9181 ccctgcgttt tttcagtaaa ctagttgtaa atcttgcatg ctccttccta cgaactcatg
     9241 aatcgtcgat tttgcccttc tagctagttt caccaattaa gtagttctac attccccgct
     9301 tttaacctac tacacctttt tttgttttca gctattcctt cttcttgctg aaagtccttg
     9361 aggttctttc ttacaggata ccatcaaggt gttcaatggc ttattcccat tgaatgaccg
     9421 agacttacta tcagatttct gctcgttgaa ccaagtcttt gatacaggat ttcttccgtc
     9481 atcttctcac aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc accctcccag
     9541 tggactgtca ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt cctcacagac
     9601 ag
//
LOCUS       DAZAJZ010000102         9379 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000102 DAZAJZ010000000
VERSION     DAZAJZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.105"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACG86D_002507"
     CDS             complement(<1..244)
                     /locus_tag="ACG86D_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN3141605.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACG86D_002508"
     CDS             complement(237..1829)
                     /locus_tag="ACG86D_002508"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN3141606.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="ACG86D_002509"
     CDS             complement(2339..3064)
                     /locus_tag="ACG86D_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141607.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="ACG86D_002510"
     CDS             complement(3218..3544)
                     /locus_tag="ACG86D_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141608.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="ACG86D_002511"
     CDS             complement(3581..3946)
                     /locus_tag="ACG86D_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN3141609.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="ACG86D_002512"
     CDS             complement(3983..4198)
                     /locus_tag="ACG86D_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3141610.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="ACG86D_002513"
     CDS             4327..4662
                     /locus_tag="ACG86D_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3141611.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="ACG86D_002514"
     CDS             complement(4738..6735)
                     /locus_tag="ACG86D_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141612.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="ACG86D_002515"
     CDS             complement(6833..7108)
                     /locus_tag="ACG86D_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141613.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="ACG86D_002516"
     CDS             7340..8074
                     /locus_tag="ACG86D_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3141614.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="ACG86D_002517"
     CDS             8113..9255
                     /locus_tag="ACG86D_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141615.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3067 a         1772 c         1360 g         3180 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactcttt
//
LOCUS       DAZAJZ010000103         9081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000103 DAZAJZ010000000
VERSION     DAZAJZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.106"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..1539
                     /gene="arcD"
                     /locus_tag="ACG86D_002518"
     CDS             118..1539
                     /gene="arcD"
                     /locus_tag="ACG86D_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN3141616.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1553..2671
                     /locus_tag="ACG86D_002519"
     CDS             1553..2671
                     /locus_tag="ACG86D_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN3141617.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2731..4023)
                     /locus_tag="ACG86D_002520"
     CDS             complement(2731..4023)
                     /locus_tag="ACG86D_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN3141618.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            4173..4733
                     /locus_tag="ACG86D_002521"
     CDS             4173..4733
                     /locus_tag="ACG86D_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3141619.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            4862..5239
                     /locus_tag="ACG86D_002522"
     CDS             4862..5239
                     /locus_tag="ACG86D_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN3141620.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5172..7244
                     /locus_tag="ACG86D_002523"
     CDS             5172..7244
                     /locus_tag="ACG86D_002523"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3141621.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(7455..8402)
                     /locus_tag="ACG86D_002524"
     CDS             complement(7455..8402)
                     /locus_tag="ACG86D_002524"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3141622.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            8648..>9081
                     /locus_tag="ACG86D_002525"
     CDS             8648..>9081
                     /locus_tag="ACG86D_002525"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN3141623.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         3011 a         1685 c         1915 g         2470 t
ORIGIN      
        1 gttcataata tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa
       61 atagaaggga atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg
      121 gaaaagaaat cgacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca
      181 atcggaagcg gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca
      241 gcgatccttt catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat
      301 catttaggag aaaagcgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca
      361 tttggtaaat taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga
      421 aatgttgctt ttgcaacgat gctgatgagc gccatgggag aatttttccc gatattcaaa
      481 ggtggacaaa atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta
      541 ttagtaaaca atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa
      601 ttagtgccat tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc
      661 actgctgatt tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga
      721 acgatctggt tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc
      781 gaaggcgcaa gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg
      841 atcattggtc tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc
      901 gtcttatctc aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa
      961 ggaatcgtgg gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt
     1021 ggttcttggt tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac
     1081 ggtctgttgc cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta
     1141 gttgttactg caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc
     1201 tatcaatttg cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg
     1261 atctatcaag tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg
     1321 attgctgtag tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta
     1381 tgtagtattg cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa
     1441 catcaaatta cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt
     1501 tcaatcgggt tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat
     1561 cgcaaatttc ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat
     1621 tgcacaaagc tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag
     1681 cgtaagtaaa tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg
     1741 atcagatgtt ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat
     1801 cttacaaacg aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc
     1861 aggagaccat gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc
     1921 tttaggtgcc gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt
     1981 agaacagcta gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa
     2041 caatcctaca ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg
     2101 tacagtagat gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga
     2161 attcttgtcg atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac
     2221 ctactccatt ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt
     2281 ttttcgaaag tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc
     2341 tgtacatgca ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga
     2401 gaaccttgtg attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc
     2461 caattacgtc tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg
     2521 tatcaattta ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc
     2581 gaagcatgcc cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct
     2641 cttatctgag ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac
     2701 aactgttcaa gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga
     2761 agctatggag actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt
     2821 attgtgtcgt acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc
     2881 gcgataacca aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt
     2941 tgcttggaaa gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt
     3001 tgtgatcaat gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg
     3061 taaggcagct aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc
     3121 aaaacccaaa ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa
     3181 tcttggagcg gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa
     3241 cgtaacattc ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat
     3301 ggaattgttg gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg
     3361 atatttcaag tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac
     3421 aaatttttca tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt
     3481 aatgaaaaag gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc
     3541 aagtaatgtt cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc
     3601 ttttggcgtc ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc
     3661 ttgaagcaga cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat
     3721 gacgattgcc attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc
     3781 taattttccc caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa
     3841 tccgctccaa aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga
     3901 cataaaaggc atgactaaac taaatccgat accagccata aatgttccaa accataagac
     3961 aaacaagttt tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc
     4021 caattagact ccttctttct gaaaattttc atctacagct ctttattata aactacaata
     4081 tgtagtttat caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt
     4141 gaaggattta ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga
     4201 aaatcatcga ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc
     4261 agcatttagc caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata
     4321 ttcgtgatgt caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc
     4381 gacgggctct tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc
     4441 gatcctttgc tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca
     4501 atgaagctgt tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg
     4561 aggcttttca attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga
     4621 tccacggtta tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc
     4681 cagaagacag ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca
     4741 gattcatttg taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct
     4801 tgcaactttc ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag
     4861 tatgtatcaa gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg
     4921 ggaagaagat attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta
     4981 cgaaaaacaa tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt
     5041 tctcagcgcc ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt
     5101 acagcaatac aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag
     5161 aaaggaattt tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa
     5221 gcaaggtgct tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg
     5281 ttacttgggc ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat
     5341 ggcagagctg gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac
     5401 ttcctaaaac aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct
     5461 cagaagcagt cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct
     5521 tagagtatgt gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata
     5581 aagcaatcaa aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg
     5641 aatctgctct tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct
     5701 taactgtgaa gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat
     5761 tagtgagaga ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca
     5821 cgttgcaagc tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa
     5881 cacaagaaga agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag
     5941 aagtccaaga aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc
     6001 aaagagaagc caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa
     6061 gtttgtatga gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa
     6121 atgacatgaa aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg
     6181 tcaagcagta tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta
     6241 aagtgaccga tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa
     6301 gtaatgaaga gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc
     6361 aaccccataa agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt
     6421 ttcggcaaaa tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa
     6481 cagtcaaatg ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat
     6541 ctgcaccgcc taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc
     6601 tgggaacccc agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg
     6661 aacgtacccc tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa
     6721 atccctcttt agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg
     6781 ctgcaggaaa gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct
     6841 tggtgaatga gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga
     6901 aatgcccaga atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg
     6961 tctgcaccaa ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc
     7021 gatgcccgca atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc
     7081 gttgtaaata cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa
     7141 tgaccaaaca tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag
     7201 aaagcccatt agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg
     7261 ttgtgataga agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa
     7321 atttttgtga aatttcggct tatttccgaa gaagctactt ctggaacacc atttattcgg
     7381 aaaaaaggac tgtgacaaga cttttgtcac agtccttttt catttaaaaa aatgtttact
     7441 tcatttgacg tatattattg gtgaggcttc aattcatttg gcttcacttt ttctgtttct
     7501 cttatgatat aaacaggacg ctgtttcgtt tctaagaaga ttttcccaat atatttcccg
     7561 atgattccaa gacataacaa ttggattcca ccaatgaaca ggaagatcga gaccatggaa
     7621 ggccatcctg atgtaggatc accaaacaac aatgcccgca aaataatgaa caaaattgca
     7681 ataccagaac cgacacaaga caaagcgccg ataaatgaag caatcgttaa cggtgcatcc
     7741 gagaagttca cgataccatc aatcgagtag ttgaataatt tccagaagga ccaagatgtt
     7801 tcgccagcca cacgttcgcg gttttcataa gaaatatatt ctgttttaaa tccgacccag
     7861 ctaaataatc cttttgaaaa tcggttgtat tctgttaatt ctaggacggc atccaccatt
     7921 tgacgagtca tcaagcggaa atcacgagcg ccatcgacca tttctgtatc gctgatttta
     7981 ttgatcaact ggtagaattt tttcgcaaag aaactgcgta tcgcaggctc gccttcacga
     8041 gtcgaacgcc gggtgccgac acaatcgata tcagaagtag tgatccggtt gatcatctca
     8101 ggaagcagtt cgggcggatc ttgcagatca acatccatca cggtcactaa atctcctgta
     8161 gaagcttgca atccagcgta cagagccgct tctttcccaa aatttctgga aaatgagaca
     8221 aagcggacat aaggataagc tgaggctagc ttcttgattg ctgctaacgt accgtctttt
     8281 gatccgtcgt caataaacaa atattcgaac tcatgggcac ctttgaattt ttcaacctca
     8341 tcaaaaaaaa gaggaacagt tttttcttca ttaaaacatg ggacaacaat tgatactttc
     8401 atcgtttcta tcctttcaat cgtatgttta tatatggttt acattcaaaa tttaataaac
     8461 cttaaataac acctttaaaa tttgatttta cgtaaaagaa caggtattct ttttgtaaac
     8521 taacgtattt agtttaccat gtttcctcgg tttatcaaac ttttaagaac aatttttatt
     8581 agttggcaag tagttttgga tagagaaaaa ggaggaagtt ccagtctatt gactaggagc
     8641 aaacaaaatg aaaatacaca cacctatcgg ctttttatgg ttagatgcag atgaatccgg
     8701 attgactgct gtatcttttc agccaatttc agaaagcgaa tcagcgaatc agcgaatttt
     8761 aacgcgcaca caagaacagt ttgaagaata ttttgcggga aaaagaaaag aatttgatgt
     8821 tcctttatct ataaagagag gtacgccttt tcagcaaaat gtctggaaag ctttgtcgac
     8881 cattccttat ggagaaatcc gaacgtataa agaagtagcc gttgctgttg atagtccgaa
     8941 agctgtgcga gcgattgggc aggcaaatcg aaccaatcca ttgccaatcg ttattccttg
     9001 tcatcgagtg atcgggcaaa atggaaaact gacaggatat atgggaaacg caatagatgg
     9061 ggttgaaatc aaacgccaat t
//
LOCUS       DAZAJZ010000104         8944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000104 DAZAJZ010000000
VERSION     DAZAJZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.107"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACG86D_002526"
     CDS             complement(13..1152)
                     /locus_tag="ACG86D_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN3141624.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACG86D_002527"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACG86D_002527"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN3141625.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACG86D_002528"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACG86D_002528"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN3141626.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACG86D_002529"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACG86D_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN3141627.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACG86D_002530"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACG86D_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN3141628.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACG86D_002531"
     CDS             complement(6166..7257)
                     /locus_tag="ACG86D_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN3141629.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACG86D_002532"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACG86D_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN3141630.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACG86D_002533"
     tRNA            8800..8871
                     /locus_tag="ACG86D_002533"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2320 a         1897 c         1648 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 agaa
//
LOCUS       DAZAJZ010000105         8671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000105 DAZAJZ010000000
VERSION     DAZAJZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.108"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(269..2650)
                     /locus_tag="ACG86D_002534"
     CDS             complement(269..2650)
                     /locus_tag="ACG86D_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN3141631.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2640..3287)
                     /gene="recU"
                     /locus_tag="ACG86D_002535"
     CDS             complement(2640..3287)
                     /gene="recU"
                     /locus_tag="ACG86D_002535"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN3141632.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3351..3890
                     /locus_tag="ACG86D_002536"
     CDS             3351..3890
                     /locus_tag="ACG86D_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN3141633.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3980..4399
                     /gene="gpsB"
                     /locus_tag="ACG86D_002537"
     CDS             3980..4399
                     /gene="gpsB"
                     /locus_tag="ACG86D_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN3141634.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4421..4788
                     /gene="rnpB"
                     /locus_tag="ACG86D_002538"
     ncRNA           4421..4788
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG86D_002538"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4997..6163
                     /locus_tag="ACG86D_002539"
     CDS             4997..6163
                     /locus_tag="ACG86D_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN3141635.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6209..7705
                     /locus_tag="ACG86D_002540"
     CDS             6209..7705
                     /locus_tag="ACG86D_002540"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN3141636.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7786..8469
                     /locus_tag="ACG86D_002541"
     CDS             7786..8469
                     /locus_tag="ACG86D_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3141637.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8478..>8671
                     /locus_tag="ACG86D_002542"
     CDS             8478..>8671
                     /locus_tag="ACG86D_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141638.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2727 a         1571 c         1843 g         2530 t
ORIGIN      
        1 atgggattat taagaagtgg cactaaataa aagactagtg tgtatttagc cgatttaatt
       61 ataaacaagt aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt
      121 ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa
      181 tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac
      241 cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg
      301 atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag
      361 gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg
      421 aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt
      481 cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag
      541 tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg
      601 tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc
      661 agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg
      721 ctacgtgtga cgattctgaa gtgacaggcg tcattttttt attgtaacct gtccacacgg
      781 aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa
      841 taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat
      901 ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt
      961 ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt
     1021 cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc
     1081 cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc
     1141 catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga
     1201 tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga
     1261 ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt
     1321 gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa
     1381 tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt
     1441 tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt
     1501 cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa
     1561 gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga
     1621 tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg
     1681 tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat
     1741 cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta
     1801 cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca
     1861 aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag
     1921 gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct
     1981 taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt
     2041 tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca
     2101 tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg
     2161 aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt
     2221 tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc
     2281 gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt
     2341 tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg
     2401 aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc
     2461 agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga
     2521 tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat
     2581 tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt
     2641 catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg
     2701 tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc
     2761 aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa
     2821 tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag
     2881 accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta
     2941 aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga
     3001 tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca
     3061 gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt tttatggata
     3121 accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc
     3181 atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga
     3241 gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt taatcacact
     3301 cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt
     3361 caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg
     3421 atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag
     3481 aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa
     3541 tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt
     3601 ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat
     3661 tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga
     3721 atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat
     3781 tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct
     3841 acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt
     3901 gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat
     3961 gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca
     4021 agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa
     4081 tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag aagaaaacga
     4141 tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt
     4201 gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt
     4261 gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa
     4321 accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga
     4381 aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc
     4441 tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg
     4501 tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag
     4561 gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc
     4621 catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt
     4681 tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct
     4741 ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat
     4801 cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag
     4861 ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg
     4921 ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata
     4981 aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag
     5041 cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga
     5101 gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt
     5161 acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga
     5221 attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt
     5281 tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat
     5341 cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc
     5401 actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat
     5461 ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg
     5521 tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa
     5581 agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt
     5641 aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg
     5701 gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga
     5761 tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc
     5821 caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga
     5881 cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct
     5941 aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt
     6001 aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca
     6061 aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta
     6121 ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca
     6181 ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa
     6241 agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat
     6301 gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt
     6361 ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga
     6421 agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga
     6481 acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg
     6541 acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca
     6601 aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta
     6661 tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt
     6721 tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga
     6781 acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt
     6841 gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca
     6901 cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac
     6961 agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca
     7021 agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca
     7081 tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa
     7141 gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa
     7201 attttatcgt tcattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt
     7261 gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg
     7321 ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat
     7381 acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt
     7441 tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt
     7501 gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa
     7561 ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat
     7621 ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta
     7681 ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa
     7741 tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa
     7801 gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat
     7861 cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac
     7921 cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa
     7981 aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag
     8041 gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct
     8101 gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca
     8161 ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt
     8221 tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg
     8281 acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat
     8341 tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt
     8401 tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac
     8461 aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc
     8521 gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg
     8581 agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg
     8641 attcggaatt ggcaggagaa caccgtttat t
//
LOCUS       DAZAJZ010000106         8334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000106 DAZAJZ010000000
VERSION     DAZAJZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.109"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..616
                     /locus_tag="ACG86D_002543"
     CDS             <1..616
                     /locus_tag="ACG86D_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141639.1"
                     /translation="KKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGI
                     EEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEK
                     NILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTAL
                     SEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            603..2024
                     /locus_tag="ACG86D_002544"
     CDS             603..2024
                     /locus_tag="ACG86D_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3141640.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            2037..3044
                     /locus_tag="ACG86D_002545"
     CDS             2037..3044
                     /locus_tag="ACG86D_002545"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN3141641.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            3056..4060
                     /gene="neuB"
                     /locus_tag="ACG86D_002546"
     CDS             3056..4060
                     /gene="neuB"
                     /locus_tag="ACG86D_002546"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFN3141642.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            4057..5205
                     /gene="neuC"
                     /locus_tag="ACG86D_002547"
     CDS             4057..5205
                     /gene="neuC"
                     /locus_tag="ACG86D_002547"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN3141643.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5178..5855
                     /locus_tag="ACG86D_002548"
     CDS             5178..5855
                     /locus_tag="ACG86D_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN3141644.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5806..6987
                     /locus_tag="ACG86D_002549"
     CDS             5806..6987
                     /locus_tag="ACG86D_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN3141645.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            7000..7977
                     /locus_tag="ACG86D_002550"
     CDS             7000..7977
                     /locus_tag="ACG86D_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3141646.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            7977..>8334
                     /locus_tag="ACG86D_002551"
     CDS             7977..>8334
                     /locus_tag="ACG86D_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3141647.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKN"
BASE COUNT         3037 a         1049 c         1515 g         2733 t
ORIGIN      
        1 caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt atgatgtaaa
       61 aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa acatacaatc
      121 aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg atcaaccttt
      181 attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa atagtatatt
      241 ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa atcgttatgg
      301 tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg aaaatgactt
      361 ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat ctatgatgtt
      421 tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat atacagcatt
      481 atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac attataaccg
      541 tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa agttggtaga
      601 gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt ttctggtata
      661 ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt tttgtaaata
      721 ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta tcaatttttt
      781 cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt gactataaag
      841 atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg ttaattgtgt
      901 tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat atgaaccaac
      961 ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt tatatccagt
     1021 cttattttac gattaacttt aaccatttta aatatttgat tttatctttt atatctactt
     1081 ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat gataaatatg
     1141 ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta atttatttaa
     1201 gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac gctttaaaaa
     1261 tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa ttcgatagaa
     1321 ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac attagtaaca
     1381 taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt ccatggttct
     1441 atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac tattatttag
     1501 tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta gcaaaattta
     1561 tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc ttaggctatt
     1621 tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa aaaacatcgt
     1681 ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac atgattttca
     1741 tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat tttttacttt
     1801 ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt gacacaaaaa
     1861 aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat attttaatca
     1921 atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta gttatatatt
     1981 ttttaaagat taagaatcga attatagatg actataattc ataggaggag atataagtgt
     2041 ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt gaaattgttc
     2101 gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt gaagaaaaac
     2161 aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta attggtgata
     2221 tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt tttcatgctg
     2281 ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta aaaacaaatg
     2341 tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa cattttgtag
     2401 gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca aagttattga
     2461 gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca atattttctt
     2521 gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt tttagtaaac
     2581 aaataaaaaa caataaagat attactttga cagatgttga tatgacacga tttatgatgt
     2641 caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa tttggagaaa
     2701 catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca ttaataaaaa
     2761 tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta ggtatcaggc
     2821 caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc tcatatgaaa
     2881 atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg tacaaagatt
     2941 atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg agtggacagg
     3001 aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt ttatgatgaa
     3061 atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc tagatttagc
     3121 tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat ttcaaacatt
     3181 taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga aggataatct
     3241 taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc aatctgattt
     3301 tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta ctccttttga
     3361 tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa ttggctcagg
     3421 agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc cagttttatt
     3481 atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt tcatagatag
     3541 tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag atattaattt
     3601 attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact ccgatcattc
     3661 attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa ttgagaaaca
     3721 ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag atcctcagga
     3781 atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg atggtgttaa
     3841 gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc tagtggtcac
     3901 tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa aacgtcctgg
     3961 aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta aaagaccaat
     4021 aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact tatagtaagt
     4081 ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact acaagataat
     4141 tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg atttactaaa
     4201 gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt gtttttagag
     4261 aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt cacaaatata
     4321 tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga aatgttggca
     4381 gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg aggcgaagtg
     4441 tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc tgttcatttt
     4501 gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc ttggagagtt
     4561 tttcccgtcg gagctcctag aatagatact attttaaata atagattacc tagttttaga
     4621 gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt gcaagtattt
     4681 catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt gatcaattca
     4741 atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc tggtagcgaa
     4801 attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat tacgaattta
     4861 gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg aaattcttct
     4921 tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg aaccagacaa
     4981 aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa tgaaattgaa
     5041 actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa tgttaagaac
     5101 atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca aatattgaat
     5161 gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat actagtgatc
     5221 catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc ttaagattag
     5281 aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa gatctgaaat
     5341 atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa atctataaat
     5401 ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa ttgaatatgc
     5461 caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa gaaatttcaa
     5521 ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct gtaattggag
     5581 agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt aataactatg
     5641 ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta ggaactcaaa
     5701 taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt atgataggcg
     5761 cagggagcac tattgttaag gatattccct cacattcctt agcttatggc aatccagcta
     5821 aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag tcctcatatg
     5881 ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat agcgccatta
     5941 ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc taagggcgga
     6001 gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact gcttaatgtt
     6061 ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt aaatcctatt
     6121 ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg gaatatgagc
     6181 cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt acctaaggca
     6241 atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt agctattgca
     6301 aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc tacttataag
     6361 ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg taataagatc
     6421 atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt tgagcattca
     6481 agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag cgaaataggt
     6541 tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat ggaagtattg
     6601 aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc tttttctgga
     6661 attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg ttggttgtct
     6721 actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat aaataaacta
     6781 aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca accagtattt
     6841 caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa gttatttaat
     6901 agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga atttgttatt
     6961 gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg aattattggt
     7021 ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa tgatatttta
     7081 ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga atttggaata
     7141 ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc agtttttgtt
     7201 tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt agattgtaat
     7261 atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga agtagctaaa
     7321 aagaaagata gattacttta tctatcttct acatttttga agagaaaaga aattcaatat
     7381 attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt aggacagtat
     7441 ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa caaaaaaact
     7501 aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac atttgggaaa
     7561 gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga ttattcggac
     7621 agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag tgtagatgtt
     7681 gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt aatagaatgg
     7741 gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat gacagatatt
     7801 caattgtacg atgaggttat gcatgataat aattattcac atactattat tgaagatgca
     7861 tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga accccaatat
     7921 acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt aaattaatga
     7981 tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa aatctaataa
     8041 gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc agtggaaaaa
     8101 aagagtttga agaaataacg tatattcaaa atagagacga tttggataca cattatgata
     8161 tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta attgttgaaa
     8221 aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat cttactcaat
     8281 ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa aata
//
LOCUS       DAZAJZ010000107         8153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000107 DAZAJZ010000000
VERSION     DAZAJZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.110"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACG86D_002552"
     CDS             387..575
                     /locus_tag="ACG86D_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN3141648.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACG86D_002553"
     CDS             653..1666
                     /locus_tag="ACG86D_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3141649.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="ACG86D_002554"
     CDS             complement(1733..2767)
                     /locus_tag="ACG86D_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3141650.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2882..5107
                     /locus_tag="ACG86D_002555"
     CDS             2882..5107
                     /locus_tag="ACG86D_002555"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3141651.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5125..7008
                     /locus_tag="ACG86D_002556"
     CDS             5125..7008
                     /locus_tag="ACG86D_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN3141652.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6989..7963
                     /locus_tag="ACG86D_002557"
     CDS             6989..7963
                     /locus_tag="ACG86D_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN3141653.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2644 a         1480 c         1712 g         2317 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2821 aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg ggagtgaaat
     2881 gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg aaaagagccg
     2941 agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag aaagcaatgt
     3001 attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata aaaattatcc
     3061 ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt ctctgtcgaa
     3121 agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa cgcccgcatc
     3181 gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca gccattatat
     3241 tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg acaagtcaga
     3301 ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt tcgatttgtt
     3361 ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa gaaatgaaac
     3421 cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg cccatactag
     3481 gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac agatttcacg
     3541 gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga gcagtcacca
     3601 tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg gagaagcgat
     3661 cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag atcagatcaa
     3721 tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat tgaatgcagg
     3781 ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat tgaacaaaat
     3841 gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc atcaatttgc
     3901 tgaacggccg attctggtca ataactggga agccacttac tttgacttca atagtgaaaa
     3961 aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg tattagatga
     4021 tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt tcgagtatga
     4081 aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca aaggattgaa
     4141 atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt atcgaacaca
     4201 tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat cacaacatgt
     4261 actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga gaaagatttt
     4321 agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt cagatgtcta
     4381 ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata tgctcgggct
     4441 ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg aaggctgttc
     4501 tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt catggacaag
     4561 tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc ttgcttatcc
     4621 gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg acagatctac
     4681 ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg aactggattt
     4741 gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat cctatcaaac
     4801 gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt ttgaagaaaa
     4861 tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt ttttagggag
     4921 gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt tagatgatga
     4981 ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat tgatgacagt
     5041 tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc atttcaaaaa
     5101 gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga tgatccacaa
     5161 gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc aacaagggaa
     5221 gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg tttttgttat
     5281 tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga tgcctgttct
     5341 tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa gatccattac
     5401 atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt cacgacaagc
     5461 cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt tggttcgtat
     5521 ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat tcattttgct
     5581 ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta tgccgaagat
     5641 tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa tattctagct
     5701 gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt tttccgattt
     5761 tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt agaagatctt
     5821 tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt agatttgtct
     5881 tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc tgatgggaag
     5941 gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat cgatggattt
     6001 gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga attggagacg
     6061 atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt tcgtaaaatc
     6121 gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa cggggtaaat
     6181 cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat ggaaaaagat
     6241 ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta ccccaaccag
     6301 atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga aacgaacctc
     6361 gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa cgtaccaggt
     6421 gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt cgaaacatta
     6481 aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc ggaagaaaac
     6541 attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt ccattatgaa
     6601 gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag ctgcatgtat
     6661 gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc gttcatctta
     6721 tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta catgacgttg
     6781 cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga tcaggcgatc
     6841 caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga ctttgacgat
     6901 aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg acaagaaaaa
     6961 cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa gtcatatgga
     7021 tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat cggggaatac
     7081 gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa acggaaaaga
     7141 atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata atgacagagg
     7201 tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc caaaagtcaa
     7261 agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc cagtaaataa
     7321 tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac tggaaatcaa
     7381 cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg gtaaaatgct
     7441 tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat tgggtttaag
     7501 gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag gggaaacata
     7561 tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac tccccgtttc
     7621 cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg tagaactaac
     7681 aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt tgcgatttga
     7741 acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag aactggaaca
     7801 ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga tttttgcaaa
     7861 agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac cttaccgaat
     7921 cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt tagattatta
     7981 tttgaaaaac ggagtttaca agacaaagct gctttattct atatgtcatg aacaagagag
     8041 taaaaaagtt ctcagatact tttgttcatg actattttct tcaataaaac caattgttat
     8101 ttttctgaaa aacaaagtta actatcagaa aattaaaaaa agtttcataa aaa
//
LOCUS       DAZAJZ010000108         8056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000108 DAZAJZ010000000
VERSION     DAZAJZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.111"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..1166
                     /locus_tag="ACG86D_002558"
     CDS             12..1166
                     /locus_tag="ACG86D_002558"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN3141654.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1188..1940)
                     /locus_tag="ACG86D_002559"
     CDS             complement(1188..1940)
                     /locus_tag="ACG86D_002559"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3141655.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2304..4970)
                     /locus_tag="ACG86D_002560"
     CDS             complement(2304..4970)
                     /locus_tag="ACG86D_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3141656.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5311..6762
                     /gene="cls"
                     /locus_tag="ACG86D_002561"
     CDS             5311..6762
                     /gene="cls"
                     /locus_tag="ACG86D_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN3141657.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7091..7906)
                     /gene="thiD"
                     /locus_tag="ACG86D_002562"
     CDS             complement(7091..7906)
                     /gene="thiD"
                     /locus_tag="ACG86D_002562"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN3141658.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2339 a         1684 c         1445 g         2588 t
ORIGIN      
        1 gaggtgttga aatgaaaata gggattgatc gtctttcctt ttttattcct aatttatatt
       61 tagatatgac agagttggca gaaagccgtg gggatgatcc tgcaaaatac catataggga
      121 ttggtcaaga ccaaatggca gtcaatcgtg caaatgaaga catcattaca ctaggagcaa
      181 atgctgccag caaaatcgta acagaaaaag accgtgagct aatcgacatg gtcatcgtcg
      241 gaacagaatc cgggattgat cattcaaaag cgagtgcggt aattatccac catttactga
      301 aaatccaatc ttttgctcgt tcttttgaag taaaagaggc ttgctacggt ggcaccgcag
      361 ctttgcacat ggcgaaagaa tatgtcaaaa atcatccaga acgaaaagta ctagtcatag
      421 caagtgatat tgctcgttac agcttggcaa gcggtggtga agtgacgcaa ggtgtcggtg
      481 ctgttgcgat gatgatcact caaaacccgc gtattttatc gattgaagac gacagcgtat
      541 ttctgacaga agatatctat gatttctggc gtccagatta tagcgaattt cctgttgttg
      601 atggtccttt atctaattct acgtatatcg aatcattcca aaaagtttgg aatcgacata
      661 aagaattgtc gggtcgagga ctcgaagatt atcaggcgat tgctttccat attccgtata
      721 ctaagatggg aaaaaaggca ttgcaaagcg tattagacca aacagacgaa gaaaatcagg
      781 aacgtcttat ggctcgctat gaagaaagca tccgttacag ccgacgaatc ggtaatcttt
      841 acactggttc attatacctg gggctaactt ctctactgga aaattcgaaa tcactacagc
      901 caggagatcg catcggtcta ttcagctacg gttctggtgc agtaagtgaa ttttttacag
      961 gttatctgga agaaaactat caagaatatc tcttcgcaca atctcatcaa gagatgctag
     1021 attctcgaac acggatcact gttgacgaat atgaaacgat tttcagtgag acgcttcctg
     1081 aacacggaga atgtgcagaa tatacttcag atgtaccttt ttcgattaca aagatcgaaa
     1141 atgatattcg ttactacaaa atataataaa atcataaaaa gaacccttca cgagtcgtga
     1201 aaggttcttt ttttatctgc ggaccaaagg agcagtctat aggtcagctt agtcgtacta
     1261 aggatttctc gtttttcact gaatagtttg cgaatgtacc tggtcacaac aggatctttt
     1321 gcgtacgcgt catagtatag cttcatcaat tcttcagtaa ttgattcctg tgtaaaataa
     1381 gtaaaatctt ttttcttcac tttgaatcct tcatcaatta agtatgtttc aaattgatcc
     1441 attacaactg gcgatgtatt aggatcttct tctatcattc ctagttgctt ttcgatagga
     1501 taaaaaacat catcgattga ttccactact ctgattatca cgcaccattt gcttgacttt
     1561 ttcatcaatt tttctaattg aataagatgt accaatgctg gatttgctgc tgtaaaaacc
     1621 atttcatatg tctcatttga ttggataaat tcttctaacg actgtttgat aaaggaaaca
     1681 tttggcaact catttttttc tgcttctttt tttgcatttt ctagcattcc tgaagaaaaa
     1741 tcgatcaaat catagcgatt aatttctttt gctaaggaaa gaaatctgcc agatcctcca
     1801 gcaacgtctg ctgctgtttc agcaggcagg actttctccg ttttcagata attttttaca
     1861 tcttcagcaa tagttacttg cgattcttgc tggatttcat aatattcttc agcaaattca
     1921 tcccattctt tttcattcat ttgatttcct ctaatttttt gttatatgtt ttcacttttc
     1981 ggaataaatc attctcctct tttatctatt ctatctcttt tgatgaataa taaaatgatt
     2041 ggctttccgt ctatcataaa taaaaaaagg gactgtgacc aaagtcgtgt caaagtccct
     2101 ctttccgaat aaacagtgtc caatcagatg accctcagta ttagctcaaa atgctgattt
     2161 tacaacttct tccttaaacg gtgttctcct gccaactctt cggtcacaag tcacaatatt
     2221 caacaaacat gagaagctgt ttgttgaaat tgcaccttgt gactcagaat taaacggcag
     2281 gctcacaacc tcgttgattc gttttattta tttccttgta ctcgtacaat gatcaattta
     2341 gtgatttcca tcaaaacaat tgtcacaagt gcgattccca tacatgtaaa gacttctgtc
     2401 aatccaaatg ctgatgggat agagaagact tctttcagtc ctgggatcat tgttaagcag
     2461 taaagcgcgc tacatacaac tacagctagt aagacacttc tgttagagaa gaaccctgct
     2521 tggatagctg tttgtgtgtt tgaacgtgct gggaatgttt gcaatgtccg acaccaaatc
     2581 aatgttgaaa atgacatggc tacaccgaat tcaggtgaat gctgcatgcc aagccattga
     2641 gcaaagataa cagcaatccc aatcagcaca ccacgatacg ttacagaaat caatgttttg
     2701 tccgtaaaga ttcctgaatt tggatctctt ggcgggcggt tcatcgtagc aggttcagct
     2761 ttttctgtac ctaatgcaat tgctggcagt gaatcattga ctaaattgat gaacaacagt
     2821 tgtaatgctg taaatggatt ggaccagtca gcaactaagg caaagatgat cgcaatgatc
     2881 gcacctaaat ttcctgcaaa tagataagaa atagctttct tgatattatc aaacacatta
     2941 cgtcctactt ctactgcgct gacaatagaa gcaaagttat catccgtcaa taccatcgct
     3001 gctgcatctt ttgccacatc tgtacccgtt cccatagcaa ttccgatgtc tgcttgtttc
     3061 aatgctggtg cgtcatttac accatcacct gtcatagctg agatatgacc tttattttgc
     3121 caagcacgaa cgatacgaat tttattctca ggtgaaacac gtgcataaac agtgactttt
     3181 tctaaaactt cgtttagttg atcttctgtc aattcatcta gttccgtccc ggtcaaagcc
     3241 aggtttcctt cttcaaaaat cccgatttgt tttgcgatag caacagctgt tgttttgtgg
     3301 tcaccagtaa tcatgattgt tttgattcct gcacttcttg cttctgttac cgcctgagtc
     3361 acttcagcac gtggaggatc gatcatagca agcaaaccaa caaggatcaa atcgtcttca
     3421 tcttcaaatg ataaatcaaa attatcaagt ggacggtaag caaaagctag tacacgtaat
     3481 gcgcgtgttg aaaaagcttc attttgctct tggaattttt tcatcaattc atccgtcatc
     3541 ggaacgactt caccatttag caatactttt ttactgcgtt ggaagacgat atctggtccg
     3601 cctttaacaa acaagtaatt tttgttatca attgtgtgtc cagtagacat taattttcga
     3661 tctgagtcaa aaggaagttc tgcttgtctt gggtagttgt ttcggatttc ctgataaggc
     3721 tggtttaatt tttcactaaa gtcgataaac gctacttccg tagggtcgcc caatttagta
     3781 ccgtcttcac tgatcgatgc atcattcgct aaaacactga tttcgatcaa gcgacgttcg
     3841 tcagctgtcc attttgaagg gtcatttgtg aaatccccag tatcgccatt acctaaaaag
     3901 tagtcaacaa ctgtcatttt gttttgtgtc aatgtaccag tcttatctgt acagatgata
     3961 cttgtcgaac caagtgtttc aactgctgga agcttccgaa taatggcatg acgttttgcc
     4021 attttattcg ttcccaaaga taacacgatt gttacgatag actgtaatgc ttctggaata
     4081 gcagctacgg ctacggctac ggcaaacatc actgcgctga cgatatctgc agtcatatct
     4141 cctgtacctt ctccaagata aatacgaaac agttggatac ctaaaatcaa taaagacaat
     4201 gccaaaatag ccaaacttag ttttttactg aatttatcca gacctcgttg taacggagtg
     4261 acttgctcag ttgttgattc caaaaggcca gcaacttgtc cgatctccgt attattccca
     4321 gttgaagtca ctaagaattt accgcgtccg taagttacga tcgttccact gaataccata
     4381 tttgtacgat ctccgattgg taccgtactt gtgatatccg ttacttcttt ttctgcagaa
     4441 gtcgactctc ctgtcagcat accttcatct acttttaatg atcccgcatc tagtaaacgt
     4501 ccatcagcag gtacataatc gcctgcgtct aacaacacga tatctcctac cacaagttcg
     4561 tttgccggaa tcgttttttc cttaccatca cgaatcactc gagcatcggg cgctgaaagg
     4621 tttttcaacg cttctaaaga accttctgct ttttttgttt ggacaacact gattatagaa
     4681 ttcaacatca atactgcaaa aataactaag aattcgatgt agtcacccat gaccatctga
     4741 atcagtgcga caatcagcaa tacgataacc attgcatctt tgaaagtttc taagaacaat
     4801 ttccaagttg gcttcttcgg cttttctttt agctggttca agccatctct ttctaatttt
     4861 tccttcgctt gcgtggtgct caatccatcg gttgagctct gcgtttgctg catcaacgag
     4921 tcgatcgttt ctttatagac cgcttgttgt gcttgttcct tgttttccaa atttcatccc
     4981 ccattttttg atcttggccc tagtcctgtc tgaaaaaaaa gagacctaag acaaatacgc
     5041 atttgtctta ggtctcacca tttcatacaa taccagaaaa ccatttggtt aacttactgt
     5101 tgatattatc gcaacaaacg ttgccggtta ctcccctaag gagatattca actgtactaa
     5161 tatcctacct tattaaccta caaaaagcaa ctactttagc ccgatttacc tacattttca
     5221 tgaaaggagt gaaaagtata ttcaccttgg taggcttctt tcttctatca tgctatacta
     5281 atgttattgt atgtaaggag gaacattttt gtggtatcta gtattataac cgccctttat
     5341 ctattaaatg cacttattgc tttggtggct attttgatca aaccccgaga tgtagcagcc
     5401 atttgggcat ggctcttagt attgtttgcc cttcctgggg tgggctttgt attatattta
     5461 tttttcggac gtggattaac ggataagaaa aaattctatc tccgacaaag tgacttgaaa
     5521 gaattagaaa actttcagtc ttttagagga gataccattg aacattacga tcctgacatg
     5581 ggcgataaag acaaacagca atttgttgac ttcttctctt cattaaatcg tatgccgctg
     5641 acaagaatga attctgtcac tcttctcaca gacggacaag agaaattgga ttcactgctt
     5701 caagatctaa aaaaagccaa acattcgatc catatcgaat attacgcatt tgtgacagat
     5761 aatatcggcc agcaagtctt acatgtttta gaagaaaaag ccgcagaagg cgtggaagtt
     5821 cgaatattat atgatgcatt tggctctcat ggcacaaaag caaaagattt caatcgtcta
     5881 atcaaaaatg gtggacatgt ccatacattt gttacctcac aaagggcatt acttcgtttc
     5941 cgattgaatt accatgatca ccgaaaaatc gttgtgatcg atggaaagat tagttatacc
     6001 ggtggtttca atattgccaa tcaatatgta aatacaacaa aaaaattcgg ctattggcgc
     6061 gatacgcata tacggatttt cggtgccgct tcttctttgc tccagcttcg cttcttaaca
     6121 gactggaacg tctcggtacc tgaagaaaaa aaggtcggct atcatttgaa ttatttcttt
     6181 aaaaaagcag atcgagatga atctaagctt gctgatacat ccatccagct tgtttcaagc
     6241 ggaccgaata acgaaaggga acaaatcaag ctttcattta tcaaattgat tacttctgct
     6301 aaaaaacgtg tttggataca gacaccttac cttgttcctg atgaaagtgt cattgctgct
     6361 ttaaaaatcg caactgcctc tggtgtagat gtgaaaatca tgattcccaa caaaccggat
     6421 catcctttta tttatcgagc aacacaatat tatgctcggc agctgatcaa ggaaaatgta
     6481 caaatccttg tctatgagaa cggcttcctc catgcaaaaa cattgataat ggatgatgaa
     6541 atctgcatgg taggttcagc aaatcaagat attcgtagct accgattgaa ttttgaaaca
     6601 agtgctgtca tttacgatcc tgagttttta gaagaacttg ctactcagtt caaagaagat
     6661 gagacacatt gttcatccat gacaactgaa acagtcaagg aaatgtctaa ctggctatta
     6721 ttcaagcaac aaatttctcg attattttct ccaatcctat aattttttta ttcaagtaga
     6781 gaaaaatata ctaagattaa agggactgtg acaaaaatct tgtcacagtc ccttcttccg
     6841 aacaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
     6901 gaagctgttt tttgaacttg ttccttgtga ctcggagctg gacggcaggc tcacgacctc
     6961 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac
     7021 ataagaagct gtttttactt ggctgttctt attacttgat gcagggcaac tttttcacaa
     7081 cttctttctt ttactctaat tcaccagctg cccaatgatt gatggggcca tatttgtgcc
     7141 ctacatttat ttcttcacga attgcacggt ttacatagtc ttttgccaat cggatcgctt
     7201 gttcaactgt ttttcctttc gctaactcag ctgcaataca tgcagatagc gaatcgccag
     7261 ttccgttgat ccggtcggta ggataaaatg gtgcatttaa ccaaaatgat tttcctgatt
     7321 cgaacaatac gtagtcattg actgtttctt gctgcgcatt tttagcatga tgtcctttga
     7381 tcataacgtt ttttgcaccc atatcttgca gttcttttgc tgcttgcaca aaatcttctt
     7441 cagctgctaa tgtccgtcca gtcagctttt ctgcttcata aaaattaggt gtcagtactt
     7501 cggccaaagg gacaagcttt tctttcaagc tttgcaaagc agattcttct agtaaaagat
     7561 ttccatgttt cgttacgatt acgggatcca cgactaatgg tccaaaatca tatttttggt
     7621 aattttttac aactgtctca atcaactttg aatctgctag catacctgtt ttagctgcac
     7681 gtatttgaaa gtccttcgca atcagttcaa attctttgtc gataaaatct gttggcaatg
     7741 tcacacttgc tccgattccg taagaattac ctgcaacaca tgcggtcatg acagaaacac
     7801 cgtaaacatt tcttgtaaag aatgtatgca tatctgcttg catccctgcg cttccatcgc
     7861 tatctgatcc tgcgatcgtc attgcttgta catactgatc ttccatcaaa tcgattcctc
     7921 catttttata aaaagatttt tcataatact gttactgttt ctatcatatg tttttcaaag
     7981 tatccctcag taaatatacc actacctttt cttggtaatc aaatttattt gaaaaaacat
     8041 ttgcaataaa tcagaa
//
LOCUS       DAZAJZ010000109         7897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000109 DAZAJZ010000000
VERSION     DAZAJZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.112"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1005
                     /locus_tag="ACG86D_002563"
     CDS             142..1005
                     /locus_tag="ACG86D_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3141659.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1134..2756)
                     /locus_tag="ACG86D_002564"
     CDS             complement(1134..2756)
                     /locus_tag="ACG86D_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3141660.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3005..3526
                     /locus_tag="ACG86D_002565"
     CDS             3005..3526
                     /locus_tag="ACG86D_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN3141661.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG86D_002566"
     CDS             complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG86D_002566"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN3141662.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5607..6425
                     /locus_tag="ACG86D_002567"
     CDS             5607..6425
                     /locus_tag="ACG86D_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN3141663.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6528..6725)
                     /locus_tag="ACG86D_002568"
     CDS             complement(6528..6725)
                     /locus_tag="ACG86D_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141664.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6894..7472
                     /locus_tag="ACG86D_002569"
     CDS             6894..7472
                     /locus_tag="ACG86D_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN3141665.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7567..7704
                     /locus_tag="ACG86D_002570"
     CDS             7567..7704
                     /locus_tag="ACG86D_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141666.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2371 a         1405 c         1568 g         2553 t
ORIGIN      
        1 ttcttgaagc tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa
       61 accggttaaa gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt
      121 aacgagaagg ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca
      181 taccatgtat tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat
      241 caagagttag tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg
      301 aaaaatggtg cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc
      361 cgttcttatg cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc
      421 ggctcttaca gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct
      481 gagatctatg tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa
      541 gctgtaagat tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt
      601 aattatttga aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc
      661 ggtaggattt ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt
      721 acggtggata aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg
      781 atccagacaa tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt
      841 tatgtggctt acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag
      901 cagataaaga tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca
      961 ggctatggct gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt
     1021 ctgtaaaaaa gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag
     1081 catcctatct cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc
     1141 cataactcgt tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta
     1201 tcagctcgat cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg
     1261 ataaattgat ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca
     1321 ttcaatgctg tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg
     1381 ttagctttcg aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa
     1441 acattcactg gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt
     1501 aagaaggtgt tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt
     1561 agatcagtat cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca
     1621 ccccaacgta ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt
     1681 gttgttgtta tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa
     1741 gatatatcat ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat
     1801 aagtcgtttc caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa
     1861 ggttggatat cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt
     1921 gattttgatg cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct
     1981 tcttttttgg catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa
     2041 aaatcataat ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta
     2101 catactttgt tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg
     2161 atcaaaaatt cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct
     2221 agcaaaagaa catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt
     2281 ccaccagtta attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc
     2341 agtaaaacag ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct
     2401 aattcagctg ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct
     2461 ttttctttca gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta
     2521 tagtcttcat agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata
     2581 gtaacggaac ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta
     2641 cctgcaccat ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca
     2701 tcatcaaata gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt
     2761 tttcctcact tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg
     2821 taaaagattc aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat
     2881 aaaaataaaa gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat
     2941 ttttgacgtt atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt
     3001 gaaaatggat gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt
     3061 acttgtctca ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt
     3121 ctttgcgatt tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa
     3181 agagctaaca ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt
     3241 tggcgtcttt ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc
     3301 agtatggttt attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt
     3361 aagtgaagga tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat
     3421 tttcttattg tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta
     3481 ttttatgtta tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa
     3541 ataaaaaagc cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat
     3601 catggacaaa tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg
     3661 agaagctata tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct
     3721 cttttcatat aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc
     3781 ttggatttct tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg
     3841 gcgcttgtta ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc
     3901 aaaggcaaat cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc
     3961 tccaacttta ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc
     4021 ttcttgttct gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg
     4081 aagctgatat ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc
     4141 acttaaggta aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac
     4201 accattaccc atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt
     4261 caataaacga atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc
     4321 ttcagcaata cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg
     4381 atcttcaaaa gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta
     4441 ccagctatca gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc
     4501 atcaccgagg atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc
     4561 gatttggata aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc
     4621 gacaaataag atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg
     4681 tccagcaaaa ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga
     4741 agcgacaaca ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg
     4801 cgttccttga ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc
     4861 tagcaaatct ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa
     4921 aggttgttcg tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc
     4981 tttatgctca taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt
     5041 catgactgta cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg
     5101 gaaagagtta tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac
     5161 atggtccatc tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga
     5221 gccgaagaca gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc
     5281 gacttcttca tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc
     5341 cataattgaa ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc
     5401 aagaactttt accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag
     5461 caatgatgat acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa
     5521 ctaattcgtc ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat
     5581 agatagattc aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc
     5641 agcaagcact tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg
     5701 tttttttctt taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg
     5761 gtgtcatgat tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag
     5821 aaaatggaga tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt
     5881 tatatatggc agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag
     5941 gattgcctac ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca
     6001 ttcctgaagg tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga
     6061 cagccgctat attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag
     6121 aaggagcagc tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg
     6181 tttacggaca agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag
     6241 taacgaaagt ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt
     6301 atgtcgtggt ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt
     6361 ttgcagtttt tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag
     6421 gataaataaa aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc
     6481 tattttttta gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt
     6541 tttctatgtg tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt
     6601 agaactttga ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg
     6661 aagctagctg caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga
     6721 cgcatacaaa accctcctct tgttcattct ttactataga aaaagtataa cagggattgt
     6781 ttaaaaacac ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat
     6841 tgctttttct ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta
     6901 aaaatcgtct tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt
     6961 tagaacttca tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat
     7021 tttttatcta tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt
     7081 tatttcaagt tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt
     7141 ttgcgctttc gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg
     7201 ctccagaaat gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg
     7261 caaaagaact tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata
     7321 attacaagaa atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt
     7381 tagtagcgcc agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc
     7441 caagtcgtac catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa
     7501 aaatatagaa aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg
     7561 atgcagatgg atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt
     7621 atttatttgg atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca
     7681 ttattcacag caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag
     7741 aaaagcatgt ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt
     7801 aatttttaga aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg
     7861 taagagataa aaataaaaaa ttagtcaaag aagggcg
//
LOCUS       DAZAJZ010000110         7883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000110 DAZAJZ010000000
VERSION     DAZAJZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.113"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG86D_002571"
     CDS             complement(220..972)
                     /locus_tag="ACG86D_002571"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN3141667.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACG86D_002572"
     CDS             1085..2005
                     /locus_tag="ACG86D_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3141668.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG86D_002573"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG86D_002573"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN3141669.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG86D_002574"
     CDS             3311..4789
                     /locus_tag="ACG86D_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141670.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="ACG86D_002575"
     CDS             5066..7126
                     /locus_tag="ACG86D_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN3141671.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="ACG86D_002576"
     CDS             7209..7778
                     /locus_tag="ACG86D_002576"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3141672.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         2806 a         1318 c         1505 g         2254 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagaaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcagtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaatca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg cca
//
LOCUS       DAZAJZ010000111         7710 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000111 DAZAJZ010000000
VERSION     DAZAJZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.114"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACG86D_002577"
     CDS             <1..107
                     /locus_tag="ACG86D_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFN3141673.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACG86D_002578"
     CDS             complement(176..904)
                     /locus_tag="ACG86D_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3141674.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACG86D_002579"
     CDS             complement(917..2197)
                     /locus_tag="ACG86D_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN3141675.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACG86D_002580"
     CDS             complement(2338..2940)
                     /locus_tag="ACG86D_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN3141676.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACG86D_002581"
     CDS             3185..3391
                     /locus_tag="ACG86D_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141677.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACG86D_002582"
     CDS             3703..4422
                     /locus_tag="ACG86D_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3141678.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACG86D_002583"
     CDS             complement(4503..5186)
                     /locus_tag="ACG86D_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN3141679.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACG86D_002584"
     CDS             5405..7591
                     /locus_tag="ACG86D_002584"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN3141680.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2768 a         1299 c         1453 g         2190 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca
//
LOCUS       DAZAJZ010000112         7593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000112 DAZAJZ010000000
VERSION     DAZAJZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.115"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..1623
                     /locus_tag="ACG86D_002585"
                     /pseudo
     CDS             70..1623
                     /locus_tag="ACG86D_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1731..2813)
                     /gene="uxuA"
                     /locus_tag="ACG86D_002586"
     CDS             complement(1731..2813)
                     /gene="uxuA"
                     /locus_tag="ACG86D_002586"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3141681.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2804..4450)
                     /locus_tag="ACG86D_002587"
     CDS             complement(2804..4450)
                     /locus_tag="ACG86D_002587"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN3141682.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4634..5296
                     /locus_tag="ACG86D_002588"
     CDS             4634..5296
                     /locus_tag="ACG86D_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3141683.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5440..6003
                     /gene="ahpC"
                     /locus_tag="ACG86D_002589"
     CDS             5440..6003
                     /gene="ahpC"
                     /locus_tag="ACG86D_002589"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN3141684.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            6013..7542
                     /gene="ahpF"
                     /locus_tag="ACG86D_002590"
     CDS             6013..7542
                     /gene="ahpF"
                     /locus_tag="ACG86D_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN3141685.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT         2432 a         1397 c         1382 g         2382 t
ORIGIN      
        1 gtctaaattt atgaaaaaaa cagtaatatt ttcaagtttt aacaatttaa gtaaaaagga
       61 gcctctttca tgcaaactca agaagcgatt aaaacgttta tcttgaagaa aaaaagcaat
      121 acaaaattag atatttttct tttcctatct gaacatcgtt tctttattac tacacaatat
      181 ttagcagatc atttccatat gtctgaatca aattttttac tatacatcaa agaattagaa
      241 caagactttg agcgtctcaa tctaacagaa ctgcatattg ataagcaaaa acctttttta
      301 aagttgaatt ttgaaggaat tgatccggct tactgctatt atcgtctatt tggaagatac
      361 tgcaacgaat cagtcagtta tcagatcttg acttctttgt tcagctgtca gacaaattcg
      421 attatcagtt tcagtcagca aacaaactat agtgcctctt atttgtatac gaaaatgaaa
      481 aagattaatg catttttagc atagtacggt ttgtctatct ccttttctaa taatggcaga
      541 aagtcttttt ctggaaaaga ggtacaaatt caatatgcag cattagatat ttattggaac
      601 atcttttcaa gtactgctac agaattttat gatgaagatc cgcaagtagt ggcttttatg
      661 atgaatactt tttttaaaaa agaagtactt gaccgactaa atagcggtat gatagataaa
      721 ttatattttc tattaaaaat atgtaaagag aattttcctg caacttctgt gaaagacgta
      781 caaaaagaat tcacagagta tgaatatatt gactttttta ttgatctctc tgtagatatt
      841 ttgcgaccta atctgccaat ttgtcgcgaa caacgcatct tagtgaatat tttagctcga
      901 ctttctatca cgaaaatcga atcggaagaa acaagcttgg agcaatacaa acttttaaaa
      961 aaagcaaacg ttcctcatgt tctttattca agagaaatgg tggagtccct ttgtactgta
     1021 tttgatttgt ttatacctga aaaagaaaag attctttact cattggcatt cgcacgaaat
     1081 aaactttata atggatttct taacttcaat caaccaaata cgccccttcc tactttcatg
     1141 ctttatcaag aacatagcgt attgaaagaa gtccaaaccg ccattgaaca attttatatg
     1201 gatttcagag aacaaaatca acacttgtta ccagttattt tagaaaagga aaatgtgcaa
     1261 tggatggttg aagacctagt acatttgtat gatcgctaca aaaaacagcc gcgaattgtg
     1321 attggtgtga actatacgcg tgatttttat atcagtaaag atctgatcgt taagatagaa
     1381 caaatttttt caaatgaatc gatcaaaata caaaatgatt ttatggagaa ctgcaacatc
     1441 gttatcagcg actgcccttt gaaacaattg ccttctcata tcaaaaaaat ctatatttta
     1501 aaaggaatca tcacaccaga agactggaaa aaagtgatta tgaggatctc agaatatatc
     1561 tttgaattaa aagaagaagc actcatggat caatcgaaaa aagaaaatca agttacttcc
     1621 tagaccaaat aagatacata aatccgaatg cttattaaca acctccggat ttatgtatca
     1681 agaatttgaa catccttctt atttacataa taaaattaca gtttttttca ctaattatct
     1741 ttacttttgt ttcctttttc gattgcttcc cataatccat ttagataaac agcacctaat
     1801 gctcgatcat ataagccgta tcctggtctt cctgtctcat tccagatcat tcttccatga
     1861 tcaggacgga tgtatccgtt aaatccgttg tcatacattg tttttagtat gcggtacatg
     1921 tctaatgaac cgtattcgga tttatgtggt gcctcataga aatctcgatt agctaaaaat
     1981 ttaatatttc gaacatgtgc aaatgggatt cgatctcttt ggcaaaattc tgctaaaatc
     2041 ccatatacat cattttctgg atcttctgca atactccctg tacaaagagt gattccgtta
     2101 taagggctgt ctactatgcg acacaaccaa tctaaatctt ctcgattctt tacgattcga
     2161 gctaatccaa aaatcgagta tggcggatca tctggatgta cagccatttt gacccctacc
     2221 tcttcacatg ttgggatgat tgattggaga aaataggcaa aattttctcg aagtttttct
     2281 tcgtctactg atgcataggc ttcaagcaag gatttgatag gagctaagcg ctcaggttcc
     2341 catcccggca atccaaattc attgctggaa tgttcgatgc gcgcgacgat ttctcttgga
     2401 tcatctggaa tatcatcaga tataaaagct aacgtggatg agccgtcttc taagcggtag
     2461 tcaaggtcag atttcaccca gtcaaataca ggcataaaat tatagcagat gacttctatt
     2521 ccatattctg ccaagtttcg gattgtctgc ttatagtttt cgatatactg ctcacgggaa
     2581 ggtaatccaa ttttaatggc atcatgcaca ttgacgcttt caattacttt tagtgttaac
     2641 ccacgagact caatttcttc ttttagttct ttgatcctct ccctaggcca tacctctcct
     2701 actggtatat ccatcaatgt gcctacaatc cctttgaccg caggaatctg tctgatttca
     2761 tctaaggtaa tcttttcttc ctgtgatccg taccatctaa aagtcatttc catgataaat
     2821 ttcctccatg ttcttgtaag taatcctgta atgtttttcg tacggcattt ggtcctgcta
     2881 ccatcttgat gaacatttct tctatttttc tgccaagctc tgcttggtaa agatctacac
     2941 caaaaatttc tttatttgac aaaataggag ccattttctc atgaatatgt gatgtatctt
     3001 gcgtacccag ttgaatatcg gaaagcttat gctgaaggtc tgtcaatagt gggtctggac
     3061 tcgggatgaa tggttttcct tgatcatcaa tgccaataag gtatcgaagc caagcagcga
     3121 gagtcaatgg tatgaataca agtgtctgcg ggtctttaac ttctatttcc atatattttt
     3181 taatcgtctc accataacga atcgccacct tttgagaggt atccgtggcg attcgctgag
     3241 gcatattagg aatatttgga ttagaaaagc gcacagtaat tacttcttcc agaaagatct
     3301 ttggatcaat gatacctgga gactccacaa ctgggaggcc ttcattgtac ccgatttcag
     3361 aaactagcag cgataaatct ggatctttca tctcctcagc tatgctccta taccccagta
     3421 aacaacccat aatagctaaa gacgtatgca aaggattaag gcaagtcgtc accttcatcg
     3481 tatctgcttt atctaccgtt gcacgatcag tcaagaatac cccagctttt tcgaatttcg
     3541 gacgtccatt gggaaaatga tcttccatga ttaaataatg gatgtcctct gtattgacaa
     3601 atggagcaat attatttcct tttgcggttt ggaaaatcga catatttgaa aagccctctt
     3661 ttgccaacat tttgctaact gacaaggaag gattcggggt gatacgatca atcatcgtcc
     3721 atggaaacgc aacaatttct tcattcgata agtattggat aaatgtattt tgtacataac
     3781 cttccttttg ccattctttt gcgatcgtta gcaaagaagt acgtaattga ctaccgtttt
     3841 gtgaaaaatt atctgtagag acaagtgcaa tcggccaagc tccagataaa aaacgtttat
     3901 aaagtagcac agcaagaata ctcattgtat gtgatggctt ctttggacca ttttttatat
     3961 cgactgcaat cggtcctata tatttattat ttgtatcttt caatcgataa cctttttcag
     4021 taatagcaaa agtaactaat tgtaaactag gattttcaaa gtgtcgaatc atcttatcaa
     4081 agtgttcaga acaatctgga tgacaataca gactgtcagc tgtcgcagct aatatacgtt
     4141 tgacgaactc tccactgctt ggcatgacca cttcaagaat caaatcatca aaatcttggt
     4201 aagccctttc aattatttct tcatcgaatg tttcacaaac aatgacacca gtggacaatt
     4261 cgcctagatc cgctaaacgt tgagcgattt ctgcatgaaa tcctcgataa agatttccgc
     4321 caccgaaatg gatccagaca ggattttttt gtccattttc tcttaatgat gcacggtcaa
     4381 aatgaggtat ttctacttct ttttgagata attcagggat aaagttcctc cagttgttta
     4441 aatcaatcat ttttcccctc ctaaactaac atattagttt ttgtattcaa gtgaatttta
     4501 tgcaataaaa atcgctatgt caaccaaaat tttactattt atacaaattt actagttaat
     4561 gtgacatacc agattgtttt tcagacctct tctgttatac tacgttcata gaaatgattt
     4621 tggaggaata aagatgaaaa gcagcttgca atcacaagct tatcaagcga ttcgtaaaca
     4681 aattatctat gctgacttag aacctggaag gaaaatatct gaaaaggcac tggaggaaca
     4741 tcttgctatc ggccggactc ccattcgcga agcattgatt caactacgtc agcaagaact
     4801 cgtgtatacg atacctcaaa gtggtacgta tgtttccaaa attaatcttg tttctgcaga
     4861 aaatgctcgc tttgtccgag aacagttgga agagcaaatc atgattgaat gttgttccaa
     4921 attggacaca caaacacgaa tagtgctaga aacgatttta gaagaacaag aaaaagcagt
     4981 caaaataaat gacgaacgcg cttttttccg tgcagataat ttatttcatg aagcttgttt
     5041 cgaaattgca ggaagaaaag aagtttggaa ctggttagat gaccataata cccatttaga
     5101 acgtttccgc tggttacgag tgaccattca ggggcttcca tgggaaacga tcatggaaca
     5161 acattatgat ttactcaagg ctttagttga taaaaaacca gaagaagctc gatttcaaac
     5221 gggattgcac ttacacatga tgctgaaaga acaggaaact gttactcaac gttatcccga
     5281 gtatttccag cagtagcatt ttcttgcgac ctcttctatt tttataatta accttcgttt
     5341 ttgagtaatc tttattaaat aacaaaatat ccattgactt tgcaactcca actaaattac
     5401 aataattata aataaataat tattattgga ggttttacta tgtcattgat tgggaaaaaa
     5461 atcgaagaat ttaatgcaca tgcttatcat caaggagaat ttttagaagt cagtgaaaaa
     5521 gatcttatgg ggaactggag tattctttgc ttctatccag ctgattttac ttttgtttgt
     5581 cctactgaat tagaagactt acaagaacaa tacagtacat tgaaatcttt aggtgttgaa
     5641 gtcttttctt gctctaccga tacccacttt actcacaaag catggcatga tacatcagat
     5701 gctatcggta aaatcgaata cgtgatgatc ggtgatccgt ctcatcagat ttctcgtatt
     5761 tttgatgttt tggatgaaga gcaaggatta gctcagcgag gaacatttat tattgatcca
     5821 gacggaatcg ttcaagctat ggaaatcaat gcagatggaa tcggtcgtga cgctagtgca
     5881 ctgattgata agattcgcgc tgctcaatat gtacgtaccc acccaggtga agtttgtccg
     5941 gcaaaatgga aagaaagtgg cgaaacattg aaaccaagtt ttgatcttgt tggaaagatt
     6001 taggtggtgg atatgttaga caaagaaact aaacaaaatc tcgaacaata tttagcactg
     6061 attgaatctc ctattgtttt ttctgttagt ttggataata gtgaaaatag tcaaaaacta
     6121 gctgaattta cgaaggaaat agcagaaatg tctcctaaaa tctcttggga aaaaagagat
     6181 tcgatgcgca cacctagttt ttctatcaat ccagttggca aagaaagtgg aatcgttttt
     6241 gctggtatcc ccctcggtca tgaattttcc tcttttttac tggctatgtt acaaattagt
     6301 ggacgggcac cgaaaatcag tgaatctttg agtcttaaaa tcaaaaaaat aacacaaaca
     6361 ttgcattttg acagttatgt tagcttgact tgtcataatt gccccgacgt tgtacaggca
     6421 ttgaatatat tagctgtatt aaatccaaat attactcata cgatgattga aggtgggatg
     6481 ttccaaaaag aaatcgatga taaaaaaatc atggcagtgc caactgtgtt tttaaatgga
     6541 gaagaattta gtagcggacg catgacgatc gaacaaattg ttgaaaaaat cactggccct
     6601 ttgattgaag aagagttgtt tgaaaaagaa ccttatgatg tattagtgat tggcggtgga
     6661 ccagcagcta gtagtgcagc tatctatgca gcacgtaaag gaatccgtac aggacttgtc
     6721 gctgattctt tcggcggaca agtcgttgaa acactaggga ttgaaaatat gattggtacc
     6781 tcttacacag aaggtccaaa attgatgcag caagtagaaa gtcatgtgcg tagttaccct
     6841 atcgacatta tgatgaatca acaggccgtt tccttaaata aagagcaaca ttttattaat
     6901 attggcttag ctaacggtat atcacttaaa gcaaaaaccg ccatcatcgc gacaggtgcg
     6961 cactggcgtt ctatcaatgt accaggcgaa aaagagttta aaaataaagg aattgcttat
     7021 tgcccacact gtgacggccc tttatttaaa ggaaaagaga ttgctgttat cggtggtggt
     7081 aattctggtg ttgaagcagc aatcgatttg gctgggttag caaaacatgt atatgttcta
     7141 gaattcttac cagaattaaa agcagatcaa atcttacagg ataaactagt ctctcttgcg
     7201 aatgtcactg ttatcaaaca tgcagcgacg aaagaaatca gcggtacgaa tcaggtagaa
     7261 tctctctcct atatcgatcg ccgtacgatg gagatgcata cactagctgt ttcaggagta
     7321 ttcattctag tcggcttact ccctaatacg gattggctag atggaacgat cgaaatgaat
     7381 ccaagaaatg aaatagtgac cgagaaaaat ggcgcaacga atatccccgg cgttttcgca
     7441 gctggtgatt gtacagatag tccatacaaa caaatcattg tttcaatggg ttcaggagct
     7501 actgcagctt taggagcatt cgattatctg atgcgcactt gagctatgaa gattgtaaaa
     7561 agtcagagag cggaaaaaag gatggtgaca aga
//
LOCUS       DAZAJZ010000113         7242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000113 DAZAJZ010000000
VERSION     DAZAJZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.116"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..575
                     /locus_tag="ACG86D_002591"
     CDS             <1..575
                     /locus_tag="ACG86D_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3141686.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            587..1507
                     /locus_tag="ACG86D_002592"
     CDS             587..1507
                     /locus_tag="ACG86D_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3141687.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1548..3005
                     /locus_tag="ACG86D_002593"
     CDS             1548..3005
                     /locus_tag="ACG86D_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3141688.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3350..3964
                     /locus_tag="ACG86D_002594"
     CDS             3350..3964
                     /locus_tag="ACG86D_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3141689.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3957..5681
                     /locus_tag="ACG86D_002595"
     CDS             3957..5681
                     /locus_tag="ACG86D_002595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3141690.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5678..7129
                     /locus_tag="ACG86D_002596"
     CDS             5678..7129
                     /locus_tag="ACG86D_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3141691.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2477 a         1206 c         1447 g         2112 t
ORIGIN      
        1 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
       61 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
      121 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
      181 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
      241 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
      301 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
      361 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
      421 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
      481 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
      541 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
      601 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
      661 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
      721 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
      781 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
      841 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
      901 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttaat
      961 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
     1021 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
     1081 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
     1141 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
     1201 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
     1261 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
     1321 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
     1381 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
     1441 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
     1501 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
     1561 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
     1621 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
     1681 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
     1741 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
     1801 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
     1861 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
     1921 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
     1981 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
     2041 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
     2101 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
     2161 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
     2221 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
     2281 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
     2341 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
     2401 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
     2461 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
     2521 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
     2581 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
     2641 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
     2701 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
     2761 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
     2821 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
     2881 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
     2941 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
     3001 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
     3061 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
     3121 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
     3181 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
     3241 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
     3301 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
     3361 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
     3421 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
     3481 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
     3541 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
     3601 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
     3661 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
     3721 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
     3781 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
     3841 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
     3901 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
     3961 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
     4021 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
     4081 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
     4141 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
     4201 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
     4261 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
     4321 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
     4381 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
     4441 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
     4501 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
     4561 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
     4621 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
     4681 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
     4741 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
     4801 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
     4861 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
     4921 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
     4981 aggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
     5041 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
     5101 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
     5161 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
     5221 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
     5281 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
     5341 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
     5401 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
     5461 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
     5521 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
     5581 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
     5641 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
     5701 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
     5761 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
     5821 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
     5881 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
     5941 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
     6001 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
     6061 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
     6121 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
     6181 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
     6241 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
     6301 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
     6361 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
     6421 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
     6481 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
     6541 acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga
     6601 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
     6661 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
     6721 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
     6781 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
     6841 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
     6901 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
     6961 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
     7021 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
     7081 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc
     7141 aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt
     7201 acttgaggca aggcagcttt ttcatgacct ctcgataaac gg
//
LOCUS       DAZAJZ010000114         7141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000114 DAZAJZ010000000
VERSION     DAZAJZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.117"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACG86D_002597"
     CDS             <1..615
                     /locus_tag="ACG86D_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN3141692.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACG86D_002598"
     CDS             612..1385
                     /locus_tag="ACG86D_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN3141693.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACG86D_002599"
     CDS             1382..1780
                     /locus_tag="ACG86D_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN3141694.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACG86D_002600"
     CDS             1965..2636
                     /locus_tag="ACG86D_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141695.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACG86D_002601"
     CDS             2695..3135
                     /locus_tag="ACG86D_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141696.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACG86D_002602"
     CDS             3147..3815
                     /locus_tag="ACG86D_002602"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN3141697.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACG86D_002603"
     CDS             3879..4502
                     /locus_tag="ACG86D_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN3141698.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACG86D_002604"
     CDS             4558..4944
                     /locus_tag="ACG86D_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN3141699.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACG86D_002605"
     CDS             5157..6548
                     /locus_tag="ACG86D_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN3141700.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DAZAJZ010000115         6909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000115 DAZAJZ010000000
VERSION     DAZAJZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.119"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG86D_002606"
     CDS             complement(<1..182)
                     /locus_tag="ACG86D_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3141701.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG86D_002607"
     CDS             517..951
                     /locus_tag="ACG86D_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3141702.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG86D_002608"
     CDS             974..1294
                     /locus_tag="ACG86D_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141703.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG86D_002609"
     CDS             1435..1626
                     /locus_tag="ACG86D_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141704.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG86D_002610"
     CDS             complement(1697..1954)
                     /locus_tag="ACG86D_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN3141705.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG86D_002611"
     CDS             2112..2375
                     /locus_tag="ACG86D_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141706.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG86D_002612"
     CDS             2399..2587
                     /locus_tag="ACG86D_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141707.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG86D_002613"
     CDS             complement(2670..2855)
                     /locus_tag="ACG86D_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141708.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG86D_002614"
     CDS             3070..3441
                     /locus_tag="ACG86D_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3141709.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG86D_002615"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG86D_002615"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG86D_002616"
     CDS             complement(3709..4137)
                     /locus_tag="ACG86D_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141710.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG86D_002617"
     CDS             complement(4197..4955)
                     /locus_tag="ACG86D_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN3141711.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG86D_002618"
     CDS             5250..5447
                     /locus_tag="ACG86D_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141712.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG86D_002619"
     CDS             5553..6035
                     /locus_tag="ACG86D_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141713.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG86D_002620"
     CDS             complement(6093..6689)
                     /locus_tag="ACG86D_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3141714.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2341 a         1061 c         1357 g         2150 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aacagctga
//
LOCUS       DAZAJZ010000116         6835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000116 DAZAJZ010000000
VERSION     DAZAJZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.120"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..747)
                     /locus_tag="ACG86D_002621"
     CDS             complement(<1..747)
                     /locus_tag="ACG86D_002621"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFN3141715.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKL"
     gene            complement(719..1615)
                     /locus_tag="ACG86D_002622"
     CDS             complement(719..1615)
                     /locus_tag="ACG86D_002622"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3141716.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1605..2765)
                     /locus_tag="ACG86D_002623"
     CDS             complement(1605..2765)
                     /locus_tag="ACG86D_002623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141717.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNA
                     EKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGIN
                     YNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
     gene            complement(2758..3387)
                     /locus_tag="ACG86D_002624"
     CDS             complement(2758..3387)
                     /locus_tag="ACG86D_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN3141718.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(3380..4498)
                     /locus_tag="ACG86D_002625"
     CDS             complement(3380..4498)
                     /locus_tag="ACG86D_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN3141719.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(4500..5132)
                     /locus_tag="ACG86D_002626"
     CDS             complement(4500..5132)
                     /locus_tag="ACG86D_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN3141720.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(5143..>6835)
                     /locus_tag="ACG86D_002627"
     CDS             complement(5143..>6835)
                     /locus_tag="ACG86D_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFN3141721.1"
                     /translation="ILISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSAS
                     AASSIIILLFINKQYSLRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNT
                     LIVGAGEGGRILYNSFLGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELI
                     EKYDIQMVTIAIPSLSRKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDV
                     VELLGRDEVELDIESIKDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGEN
                     SIYLIDRELRTHHQKCPTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLME
                     YNPKEAVKNNIFGTKNVAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGL
                     NEEGCTKFSAVRFGNVLGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLV
                     IQSGALAKGGEIFILDMSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEEL
                     LLDKERNDEAVYEKIFVGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
BASE COUNT         2125 a         1308 c          953 g         2449 t
ORIGIN      
        1 taatttatga actaaaaaaa gatttgctga aaaaaagaga gcaaacatca ctacgtgaga
       61 aatcgatgta ctgtttctat caaaatatgc atcatacttc gtatttataa atattgaact
      121 gatataactt tgtccaatag agaataggag caagctaagg attgtaacat atccagtaat
      181 ctttttatta atttttacaa taaaaaaagg gagataaaaa tatgcaagat tatggaacag
      241 tccagccact aagataaaaa aaatatactt aaatatagct ttgctgcctc gcaatttagt
      301 tagatgataa aaagctagta atatgaatat caatgcgcta aacatacgta cttgtattgc
      361 ttgtgaagta aaaccaaaaa taaaaaacaa gaacaaaact aaaaaaacat tagttgtgaa
      421 taatctgatg acatacaata aagctgagag taagacagta gaaataatca ataaaaaaat
      481 ctgatactga ctggaaataa aggtacatac gtacataaca gctctgaatc ccggttcaat
      541 actatctgaa ctaaagtcca taccaaattg tgctatccca ttatacaaga cagaatatgc
      601 ataataatca gcgttttgtg tgttaaatgc catgatgatc cacattatca aaagtagcac
      661 cccagacaaa atcttgttcg atgtactcca ttttttttgt gaaacactct tttcttgatt
      721 aatctccaat gacgcctctg tatccatctc gaacccccct tatagccgct ctaaatctac
      781 tgagcttatc agtctcaaaa gctattgcaa taaaaaattt caataatctt ttattcataa
      841 ggcgggaaaa ttccttatca cttttataca ttcttttcac caatgttgaa tttcttgcaa
      901 taaaatactt tcgtgttgaa ttatgattgt ctattttaat tttttttgat cctattttta
      961 aattttctcc atctccaacg tgatgataaa aatagacttg atttgttaca taaatgttat
     1021 agccattatt tgcaaccctt ttacaaaatt caacatcaat ctgatcaata aaaaattcat
     1081 ccttcatatt tccaacatcg tcaattaatt cacttttgaa aaacattcca gaggaatttg
     1141 caaatgctat tttcatggaa ccactagttg aagcaaagtg tttttttacg tatctccctc
     1201 tctcattttt gcaggtaaaa gagacagatt catcattgct attctcaaag taattgggaa
     1261 caataatccc aatgtcctta tctttcgctt caatattggt aaactcttct aataatttta
     1321 aagaaagatt cttttgtatg atggtatctt gatctagtaa aaggatataa tcacatttct
     1381 cttttttggc aatttcaata cctatgtttt gagctgtcgc aagcccaatg ttctcattca
     1441 ttttttttaa aattaagttt tccatcattt caaattgaaa agagttaata ttatctgagc
     1501 cgttatccac gataataagt ttgttctgtt tctcttgtat taacgtatcc actaataact
     1561 ttatgttatt atctggattg taggtaacaa taattatccc aattttattt ttcatcgttc
     1621 atcaccttgt acacttcatt ccatttcatt ttcaatactt gctcagagaa tatagtggcg
     1681 taaatttttc taacattttc atttatgttt ttattacact gataaaattt caacatctca
     1741 ttttcatcaa cggtactatt ataattgatt ccgacattat atcttttaac tatttctttc
     1801 gtatctcctg ataaatttga aattataggt aatccaaacc taaaatactc aatcgatttc
     1861 agacttaaac caattatggt atttgactta aataaattta gtccaaaatg acatttatcg
     1921 agtataactt ttttttctgc attgtcatat ataacaccgc agtcaacaaa ttcaatatga
     1981 tttttttcac aaatatcagc taatttagtt ttgctttcac cttttccaat aaagtaaaga
     2041 tttattttgt ttccaaattt tcttttgcat gttttcaata attcattaat cgcttctaaa
     2101 tctacgatat tattaatcgt acctaaataa gcaaggttca acgtagactc taagctttta
     2161 ttgactattt tctctccata atcattttgg tcatctacat tgaaagaaca tggataaatg
     2221 gtcatatttt ttgattctcc ggaaattttt atgaattttt tgaaataatc gcattctgtt
     2281 ataactagac tagaattact gagatattga tctcgtagct ttttccataa atcagtaaaa
     2341 aatttatatt tgttccctat tcccggcatt gactctggcc ataaatcata cacatcaaaa
     2401 ataactcgat tgcatctact aaacttagca ataaaaaaaa ctaaggtatt aggtggtatt
     2461 ctacaataaa taatttcagg cttttctttt cgaataaata ataatgtttt tattgaaaac
     2521 catatatgag ataaaattcg cattacggat atattttttt tatatccgat cgtttttatc
     2581 acatgacgat ttttaatcat atgcctttct ttactggata gaaattgttt tcttatatgt
     2641 gagtaatcag atacaacatt aaaatattcg atactgtttt ccgtcagaat ttcctctaat
     2701 atattcgttc gactgatata atcaaaagga tgaataagca gccacttttt tttattctca
     2761 ctcatctatc ttcgcaccct caaatttctc agtagtccca cttgctactg atgttattcc
     2821 atcacggaca ataactttct ttagcgttaa aaaaaatatt tttatgtctt tcatcattga
     2881 aatgttctcc acatattcaa tatctttttc aaatttttct ttccaactaa ttgcattacg
     2941 accactgact tgagctaatc cggttaaacc tgggcgcaca aaatgtcttt ttcgttgttc
     3001 attactgtat agtgaaagat actcaactaa cagaggacgc ggtccaataa tactcatatc
     3061 tccttttaaa atattccata attctggtag ttcatcaaga ctagtcgaac gcaatatttt
     3121 accaaattga gtcaatctga tatcgtctga taataaatta ccttcttcat ctctttgatc
     3181 tgtcattgtt cgaaatttat acatcagaaa aatcttttca tgttttccag gtcgttgttg
     3241 tttaaagatc actggcgatc caagctttat tcgaactagg attgcaacaa acgtcatcag
     3301 tggccataac attattaaac ctaaaattga aaaaagaata tctatcaaac gtttaaagaa
     3361 cttttcgtag atacctctat tagacatttt gcatcactcg cttaattgta ttagttactc
     3421 gatacaaatc atcatcggtc attttagaat ctgaaggtaa acagatacct ttttcaaaca
     3481 atgtctgagc aacatctgta ccaatatact catacttatc gaaaactggc tgtaaatgca
     3541 ttggtttcca tatcgggcgg gcttctatat tatttttatc taactcatca ataagatgat
     3601 tcgtatttat tccctttact gttatgacac ttaaccaata atttggttca ttccaattat
     3661 tttctggcat gaattgtata tttgacaatt tagacaattc ttttttataa aaactatgaa
     3721 tatatcgttt tttctcaatt ctttcattta aaactttcaa ttgtcctcga ccgatacctg
     3781 caacgatatt gctcattcga taattatagc caatttcatt gtgttgataa tggcgtgcct
     3841 tctcacgaga ttgcgtagcc caaaacctca ctttttccac cttttctttg tcattagaca
     3901 caagcattcc gccacctgaa gtcgtgatta tcttatttcc attaaaagaa taaattccat
     3961 agtcgccgaa tgtaccagta tattttcctt tatatgttgt tcctaatgat tctgcagcat
     4021 cttcaattaa aacaacatta tgttctttgc atattttagt aatttcatct agttcagctg
     4081 ataaaccgta caaatgcact actaatacag cttttgcgtt aggatatttt tctagcgctg
     4141 ttcgcaaatg ttcagggttc atattccaag tttcgtaatc actatcaaca aatactggaa
     4201 tagctccttg atagatgatt ggattggcag tagctgagaa cgtcaaagac tgacaaatca
     4261 ctatatcatc tttaccgacc ccagccgctt ttaaagctag atgtatagct gctgttccag
     4321 aagatagtgc agctgcatga ctagcaccga catattctgc taattcctct tcaaaaccat
     4381 tgacattcat acccaaagga gccacccaat ttgtatcaaa tgcttctttt acatactctt
     4441 gttcatatcc ttcatcactc atatgaggag atgctaataa tattcgttta ttttccaact
     4501 taatcccatc tttctatgac tttggtaggt acgccaacat ttttagtctt attattaata
     4561 ttttttatta ctacacttcc cgcacctata atgttcatat ttccaattct tagattttgt
     4621 attattttag tacctactcc aatttcattt ttatctccta atagaacatg tcctgaaata
     4681 tttacacttg gaaaaattga attataatta ccgatttgag tatcatgtcc gatagtagag
     4741 ccgatattga tcatattgaa atttcctaaa actacgtcag cagtgtaagt cgtatagggc
     4801 atcaaaatat tgccaattcc taactgaaca tttatgccca ctagtgcact tgatgcaact
     4861 aaattaggga aaatcaattt attattttta tttatctttt gatatatttt ctctttgata
     4921 tctggcgaag cgattcccag aaaaatagca gtttcttctt ctaattctaa taacgagtct
     4981 attgttccca atacagtttt tcccactaat tcttgatttt gcatttttaa cgaatcatcg
     5041 acaaaaccaa tcaattcata tctagagaaa ctttctagta gaaaagcaat ctctttgcca
     5101 aatccaccag ctcctacaat aattgctttc actttactcc ccctacttat tcgaagcatt
     5161 cgcaaaagtg acaatgtctt tagccaattg ttcatcgtct tgcggcaggc tcttgacaaa
     5221 ttccatcact gtttgcatgg aataaccttt gatgtttccc acaaagatct tctcgtaaac
     5281 tgcttcatcg tttctttcct tatctaacaa tagttcttca tatagttttt cccctggacg
     5341 gatacctgtt tcaatgattt cgatctcgtc ttcgctatat ccgctcaatc ggatcatgtt
     5401 ttttgctaga tctacgattt tcactggttc actcatatct aaaataaaga tttcgccgcc
     5461 ttttgctaac gccccagatt gaataactaa acgacttgct tcaggaatcg tcatgaaata
     5521 tcgggtcata cgaaagtctg ttacagtgac tggccctcct tgagcaattt gttcccggaa
     5581 tactggaatc acactaccgc gagaaccaag gacattccca aatctgacag ctgagaattt
     5641 cgtacatcct tcttcattca acccagtcac gatcatttca gcgattcgtt ttgttgaacc
     5701 catgacgttt ggcggattgt tggctttatc tgtcgagacc atgacgaagt tttttacttt
     5761 cgctgctttg gctgcttctg ctacattttt tgtaccgaaa atattgtttt tgactgcttc
     5821 ttttgggtta tattccatca atggtacatg cttatgagcg gctgcatggt agacgatatc
     5881 tgggtggtat tgttccatga tctcattgat tttcttgcga tcttggatat ccgcaatgat
     5941 cggcacgatt tctgtaggac acttttgatg atgcgtgcgt aattcacggt caatcaagta
     6001 aatcgagttc tccccatgac ctaaaagtaa tagttttgca gggtcaaatt ggatgatttg
     6061 ccggcagatt tctgaaccga ttgatcctcc tgcaccggtg acaagaatga ctttgtttgt
     6121 gatctggtct ttgatggatt cgatatccaa ctctacctca tctcttccca gtaattccac
     6181 tacgtcgatg gtttttaatt ttgatacgct gattttccct gaggcaagtt cttccataga
     6241 aggcatcgta ttgactttca catgagcaga ttcgactaac tcgaagattc ttcgtaattt
     6301 ttttcttgat aaagaaggga tagcgatcgt taccatttgg atgtcatatt tttcgatcaa
     6361 ttccggtata tcttttaatg accccagtac tttttttcct gaaagatagg tatttctttt
     6421 attcggatca tcatccacga atccaatgac atggatatct tgagccgttt tagaaccaag
     6481 aaagctgttg tataaaatac gtccaccttc gccggcccca acgatcaagg tatttttagc
     6541 ggaatctgtt gatagatacc tcatattttt tgtttcgata tagatacgcc agatcaatcg
     6601 gctgccaatg atcagtaata gcgaaagtag gtatgcaaag atcactaaac gtaaactgta
     6661 ttgtttattt atgaaaagca atatgatgat acttgaggcc gcacttgcag ataacgaacc
     6721 aaaaatcgca atgatctctc ttaaatttgt ataacgattg attcgagtaa aaactttgaa
     6781 taaactacca tagaataaat aaaaaccaat cgacaaaccg atagagatta aaata
//
LOCUS       DAZAJZ010000117         6675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000117 DAZAJZ010000000
VERSION     DAZAJZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.121"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG86D_002628"
     CDS             <1..435
                     /locus_tag="ACG86D_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN3141722.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG86D_002629"
     tRNA            complement(759..842)
                     /locus_tag="ACG86D_002629"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG86D_002630"
     CDS             1370..1870
                     /locus_tag="ACG86D_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3141723.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG86D_002631"
     CDS             complement(2060..2404)
                     /locus_tag="ACG86D_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN3141724.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG86D_002632"
     CDS             complement(2558..3478)
                     /locus_tag="ACG86D_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3141725.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG86D_002633"
     CDS             3665..4156
                     /locus_tag="ACG86D_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN3141726.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG86D_002634"
     CDS             complement(4180..6555)
                     /locus_tag="ACG86D_002634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN3141727.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
BASE COUNT         2050 a         1332 c         1118 g         2175 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttg
//
LOCUS       DAZAJZ010000118         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000118 DAZAJZ010000000
VERSION     DAZAJZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.122"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG86D_002635"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG86D_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG86D_002636"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG86D_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN3141728.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG86D_002637"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG86D_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG86D_002638"
     CDS             complement(2143..2373)
                     /locus_tag="ACG86D_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN3141729.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG86D_002639"
     CDS             complement(2603..3421)
                     /locus_tag="ACG86D_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3141730.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG86D_002640"
     CDS             3582..4271
                     /locus_tag="ACG86D_002640"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN3141731.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG86D_002641"
     CDS             4285..5787
                     /locus_tag="ACG86D_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3141732.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG86D_002642"
     CDS             5800..6276
                     /locus_tag="ACG86D_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN3141733.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG86D_002643"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG86D_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZAJZ010000119         6621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000119 DAZAJZ010000000
VERSION     DAZAJZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.123"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG86D_002644"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACG86D_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG86D_002645"
     CDS             complement(1878..3080)
                     /locus_tag="ACG86D_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141734.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG86D_002646"
     CDS             complement(3140..3343)
                     /locus_tag="ACG86D_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3141735.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG86D_002647"
     CDS             complement(3485..4552)
                     /locus_tag="ACG86D_002647"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3141736.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG86D_002648"
     CDS             complement(4610..4717)
                     /locus_tag="ACG86D_002648"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141737.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG86D_002649"
     CDS             4880..5692
                     /locus_tag="ACG86D_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141738.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG86D_002650"
     CDS             complement(6066..6257)
                     /locus_tag="ACG86D_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141739.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         2016 a         1331 c         1034 g         2240 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat g
//
LOCUS       DAZAJZ010000120         6607 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000120 DAZAJZ010000000
VERSION     DAZAJZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.124"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..474)
                     /locus_tag="ACG86D_002651"
     CDS             complement(16..474)
                     /locus_tag="ACG86D_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN3141740.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(487..1818)
                     /locus_tag="ACG86D_002652"
     CDS             complement(487..1818)
                     /locus_tag="ACG86D_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN3141741.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1829..3271)
                     /locus_tag="ACG86D_002653"
     CDS             complement(1829..3271)
                     /locus_tag="ACG86D_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3141742.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3258..4022)
                     /locus_tag="ACG86D_002654"
     CDS             complement(3258..4022)
                     /locus_tag="ACG86D_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN3141743.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4126..5001)
                     /locus_tag="ACG86D_002655"
     CDS             complement(4126..5001)
                     /locus_tag="ACG86D_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3141744.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5256..5340)
                     /locus_tag="ACG86D_002656"
     tRNA            complement(5256..5340)
                     /locus_tag="ACG86D_002656"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5304..5306),aa:Leu,seq:caa)
     gene            complement(5358..5429)
                     /locus_tag="ACG86D_002657"
     tRNA            complement(5358..5429)
                     /locus_tag="ACG86D_002657"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5395..5397),aa:Gln,seq:ttg)
     gene            complement(5439..5511)
                     /locus_tag="ACG86D_002658"
     tRNA            complement(5439..5511)
                     /locus_tag="ACG86D_002658"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5476..5478),aa:His,seq:gtg)
     gene            complement(5573..5657)
                     /locus_tag="ACG86D_002659"
     tRNA            complement(5573..5657)
                     /locus_tag="ACG86D_002659"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5621..5623),aa:Leu,seq:caa)
     gene            complement(5678..5748)
                     /locus_tag="ACG86D_002660"
     tRNA            complement(5678..5748)
                     /locus_tag="ACG86D_002660"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5714..5716),aa:Cys,seq:gca)
     gene            complement(5794..5865)
                     /locus_tag="ACG86D_002661"
     tRNA            complement(5794..5865)
                     /locus_tag="ACG86D_002661"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5831..5833),aa:Gln,seq:ttg)
     gene            complement(5877..5949)
                     /locus_tag="ACG86D_002662"
     tRNA            complement(5877..5949)
                     /locus_tag="ACG86D_002662"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5914..5916),aa:His,seq:gtg)
     gene            complement(5952..6025)
                     /locus_tag="ACG86D_002663"
     tRNA            complement(5952..6025)
                     /locus_tag="ACG86D_002663"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5991..5993),aa:Trp,seq:cca)
     gene            complement(6032..6112)
                     /locus_tag="ACG86D_002664"
     tRNA            complement(6032..6112)
                     /locus_tag="ACG86D_002664"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6076..6078),aa:Tyr,seq:gta)
     gene            complement(6114..6189)
                     /locus_tag="ACG86D_002665"
     tRNA            complement(6114..6189)
                     /locus_tag="ACG86D_002665"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6154..6156),aa:Phe,seq:gaa)
     gene            complement(6225..6297)
                     /locus_tag="ACG86D_002666"
     tRNA            complement(6225..6297)
                     /locus_tag="ACG86D_002666"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6262..6264),aa:Asp,seq:gtc)
     gene            complement(6307..6379)
                     /locus_tag="ACG86D_002667"
     tRNA            complement(6307..6379)
                     /locus_tag="ACG86D_002667"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6344..6346),aa:Val,seq:tac)
     gene            complement(6395..6466)
                     /locus_tag="ACG86D_002668"
     tRNA            complement(6395..6466)
                     /locus_tag="ACG86D_002668"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6431..6433),aa:Glu,seq:ttc)
     gene            complement(6481..6570)
                     /locus_tag="ACG86D_002669"
     tRNA            complement(6481..6570)
                     /locus_tag="ACG86D_002669"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6534..6536),aa:Ser,seq:gga)
BASE COUNT         1978 a         1460 c         1083 g         2086 t
ORIGIN      
        1 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
       61 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
      121 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
      181 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
      241 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
      301 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
      361 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
      421 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
      481 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
      541 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
      601 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
      661 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
      721 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
      781 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
      841 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
      901 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
      961 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     1021 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     1081 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     1141 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     1201 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     1261 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     1321 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     1381 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     1441 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     1501 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     1561 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     1621 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     1681 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     1741 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     1801 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     1861 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     1921 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     1981 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     2041 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     2101 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     2161 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     2221 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     2281 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     2341 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     2401 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     2461 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     2521 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     2581 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     2641 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     2701 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     2761 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     2821 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     2881 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     2941 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     3001 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     3061 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     3121 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     3181 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     3241 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     3301 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     3361 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     3421 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     3481 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     3541 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     3601 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     3661 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     3721 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     3781 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     3841 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     3901 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     3961 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     4021 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     4081 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     4141 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     4201 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     4261 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     4321 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     4381 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     4441 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     4501 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     4561 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     4621 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     4681 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     4741 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     4801 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     4861 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     4921 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     4981 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     5041 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
     5101 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
     5161 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc
     5221 aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc
     5281 acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
     5341 aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
     5401 cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc
     5461 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa
     5521 agatgttatt taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg
     5581 gccggggtcg aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc
     5641 aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg
     5701 gtgatgaagg ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta
     5761 tcaatctata aaataaagaa taaattatag aaactggggt agctggattc gaaccaacgc
     5821 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg
     5881 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
     5941 cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc
     6001 tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac
     6061 cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc
     6121 gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc
     6181 taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat
     6241 cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt
     6301 tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
     6361 ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg
     6421 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta
     6481 cggagagtaa gggattcgaa cccttgagac agtttggcac catctacatg atttccaatc
     6541 atgctccttc ggcctctcgg acaactctcc agagtgaaag aagctcaata ctctttctca
     6601 aaatgag
//
LOCUS       DAZAJZ010000121         6604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000121 DAZAJZ010000000
VERSION     DAZAJZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.125"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACG86D_002670"
     CDS             complement(1273..1938)
                     /locus_tag="ACG86D_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141745.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACG86D_002671"
     CDS             2064..4100
                     /locus_tag="ACG86D_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN3141746.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACG86D_002672"
     CDS             4104..4742
                     /locus_tag="ACG86D_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141747.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACG86D_002673"
     CDS             4770..5198
                     /locus_tag="ACG86D_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141748.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACG86D_002674"
     CDS             5655..6566
                     /locus_tag="ACG86D_002674"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3141749.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2272 a         1000 c         1219 g         2113 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aata
//
LOCUS       DAZAJZ010000122         6347 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000122 DAZAJZ010000000
VERSION     DAZAJZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.126"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACG86D_002675"
     CDS             290..1135
                     /locus_tag="ACG86D_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3141750.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACG86D_002676"
     CDS             1416..2636
                     /locus_tag="ACG86D_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN3141751.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACG86D_002677"
     CDS             2633..5353
                     /locus_tag="ACG86D_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3141752.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACG86D_002678"
     CDS             5337..6281
                     /locus_tag="ACG86D_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3141753.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1777 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttc
//
LOCUS       DAZAJZ010000123         6322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000123 DAZAJZ010000000
VERSION     DAZAJZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.127"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2513
                     /locus_tag="ACG86D_002679"
     CDS             <1..2513
                     /locus_tag="ACG86D_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN3141754.1"
                     /translation="KLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPK
                     RKEGSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETI
                     LTIQNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLS
                     IPDNIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTY
                     TIKEGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFV
                     KKYPLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNI
                     EQKVITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQR
                     ENAPIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALV
                     HKYVLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASL
                     FGNPTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERL
                     RLDSNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEK
                     VKSLSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLAL
                     IYSGALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHK
                     FLTNSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDS
                     GNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2695..2934
                     /locus_tag="ACG86D_002680"
     CDS             2695..2934
                     /locus_tag="ACG86D_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141755.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2950..4323
                     /locus_tag="ACG86D_002681"
     CDS             2950..4323
                     /locus_tag="ACG86D_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3141756.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4327..5961
                     /locus_tag="ACG86D_002682"
     CDS             4327..5961
                     /locus_tag="ACG86D_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3141757.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6124..>6322
                     /locus_tag="ACG86D_002683"
     CDS             6124..>6322
                     /locus_tag="ACG86D_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141758.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2212 a         1033 c         1284 g         1793 t
ORIGIN      
        1 agaaattgtc tgaaggtaca gtaaacataa cttataatcc tattaaacaa gaggtgatta
       61 cacaaaaaaa gcattctgtg agagacaatg atattgaaaa aattcggttt gtatttattg
      121 atattgaccc taaaaggaaa gagggaagtg caacagatag cgaaaagaaa aaggcagaaa
      181 atgttatgga acaagtagaa cgttatttga aagaaaagag aatagaatca tttgtaaaag
      241 ttgatagtgg gaatggctac catatttttc ttcccataaa tgaacaacca aataatcacg
      301 agacgattct tactattcag aattttcttc aattacttca ccgaagattt ggagtagaag
      361 atgaagttga tattgatact tctgtttata atccttcaag attatgcaaa ttagttggta
      421 caccagcggt caaaggaaag tccacaaaag agcgtccgca tcgtttatct agtttccttt
      481 ctattcccga taacatagat agaagtaatt gttttaaaat tcttgaacag atagttgaag
      541 aaaattccat gatgtcaacc tctagagtaa aaagccatcc atcttttaga gaagtgttac
      601 cttttattca agcagactat aaacaatggt tagctcacta tcctgaatta acttatacca
      661 taaaagaggg cgatcataaa ggagttacct tacttatatt tgacgaatgt ccattaagaa
      721 aacacacgaa taattctaat ggttcttgtt tgtctgttac ggctgataat aagattcgat
      781 ttcattgtct gcatgcttcg gataaaaata aagatattca agactttgta aaaaaatatc
      841 cgttgccaaa acctgtaaat ccttctgtag aatcattaga atccaatcaa gaatattatt
      901 ttgaccagtt taaactgaat caagaaggag tatttttggt gaaagaaggt caagaagaaa
      961 aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa aaagtcatta
     1021 cacttgttct tgcctattat gtcgatcatt gttgggatga aatagaaata gaaggtggat
     1081 gtttacaaac taataatttt aaacaattag caaaacatgg cttaatcatt aaagctagaa
     1141 gagaagctga agtcgttgac tttttacaga ttcaacggga gaatgctcca atcttttttg
     1201 aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat gatttttcag ttacaagatt
     1261 cttttggacg tgaaattcct tctgtattaa ctaaaaactc atgctaccaa ttaacaacta
     1321 atggtacaga agcggaattt gacgcattgg ttcataagta tgttttagga acgaacttag
     1381 aacttgcttg gtcgattggt attgcttcta ttgtgttagg ctatttaaac gttacgaagg
     1441 ctaatacttt tttgagttta ctaattaacc tacagggcaa gagcacaacg ggtaaaacca
     1501 ctacgttaca ctttattgcc tctttatttg gaaatccaac tacggttctt agaaatatga
     1561 atgctaccaa taaagccatt ataaagctag catcaaacaa tcagggaggt gttccactaa
     1621 tactagatga gttaggagca gcaactcaaa ataactttag ctcgttgatc tatcaattag
     1681 cttcaggaga agaaaggcta agattagatt ctaattgtca gcttatgcca caagagactt
     1741 tttctgtttt gacccttacc tcttctgaag agcgtttgga aaattatttc aatgagaaat
     1801 taactggttt gaaagttcga tatatcgaat tttctgatat caagtttaca aaggatgcga
     1861 atcacgcaga gaaagttaaa agtctttctt ctaatacttt tggttggggt ataaagttat
     1921 ttcttttgaa actctttgaa caagggatta caaaaattga agcaactttt gtcgaagctc
     1981 aaaaaaaact atgggctgtt ttacctgaac attctttgaa gcaacgcatt ggtaataatc
     2041 tagctttgat ttatagtgga gccctcctaa ttcaagatat actgggctat cctttacgat
     2101 tagagcatat acagacggtg cttattaatt cgtacgaaca gttaattgta gatactagtt
     2161 taggaagtga gcaaacggta tttgaccgag tgaaaacatt gttggtgatg aatagccata
     2221 aatttttaac aaacagtgac aacaaacata cgcccagtac gatttgggga aaagtatcaa
     2281 ttcaaaaaaa tggggatatt aaggtaaata tttttcctat cccgtttgag gacttgttaa
     2341 ggcgagaatt tcaagtaaag gatctaacct atctttttag gcaattgctt gattcgggta
     2401 atatgtgtgc agaaaaaggg agaagaacga aacgtattca catgaatcgt caagttcttc
     2461 caacatacga gattctatta ccttcttcac tcaaatcgta ttttcgattg taatttagaa
     2521 taaaccaaag tatgaaagtg aggcaccatg ttatggaaat atttcttatt gatttagcaa
     2581 aacaactgat tacttatctg tatgatagcg agcaaaccca aacggaagta aatgaagctt
     2641 gatctatgct tcatttactt cattcaatca aacatgaaag aaaggaaaat tctaatgact
     2701 aaaaataaaa tgacactaaa agcagaggtt cttctgtata ttcaagaaca tttttcaaat
     2761 caagcctttt ttacaaaacc aatttacttg gatttcgaaa taagaggggt atctgctgga
     2821 tctatcgggg gtactcttca agcgttgaag aacgaaggat atttagaaaa tcactttgtg
     2881 caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt tagttcattc atagagaggg
     2941 gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga agcgaaaatt
     3001 agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt tagagaaata tgaacaaatt
     3061 ccccaacgat tttcagacaa aggagaatca ggggaaaaag cggagcgtcc agcttttcga
     3121 cagcttagag cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt ttggcgttat
     3181 gatcgattgg cacgtcaagc caaaatagcc ttagaattta ttgagttgtg ccaacaacat
     3241 caggttcaag tgtattcgat ttctgaacct cttcctgctt ctttctcaca cgcgtcaacg
     3301 aataaactat ttgtgcaatt attgttcact tttgcagaac aacaacgaaa cgtcattatt
     3361 gagaatgttc gtgccggact ttcctataaa aagcaacaac ggcaatattt gtcttctgcg
     3421 gtaccatatg ggtatcgttt agtgaatggg aaaatccaac aagaagtaca cgaagcaagg
     3481 attgttcgct atatttttca actttatttg accaaaaaat atggctacaa aaagctttgt
     3541 caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc ctatcatatt
     3601 tatagtatcc taaaaaatcc attgtactat ggcgaaatca aaggaggatc tttggggaag
     3661 tatctaggaa cctttgaacc gattctctcg aaagcaacct ttttacaagc ccaagagatt
     3721 cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt acttattacg tcaaaaaatt
     3781 cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc aatggaatac aaagaagacg
     3841 aagacgcttc attattatca ttgtaccgac cgaagctgcc aaggaatttt tcttcgggca
     3901 gaaagggttg agtcagaagt attggaaaag atacaggctt tttttcaata cgaaggcgtc
     3961 tatcaagcct tgattgccca attaacgcaa caattagccg aaaagcgaaa agatgaaaaa
     4021 cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt tgaagagggc
     4081 aattgttcac tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc gatgtctact
     4141 tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga aagaatggct tcaactaaaa
     4201 gacctctcta gtcgacaatt attatgtgac tatgtttgtc ggattgaggt aacaaaggat
     4261 aaaacagttt gtgggattta cttccgacaa ctgacacaaa atattttaga aaggaacgaa
     4321 tgatctatgg aaatgataca aaccaaacga gtcgcattgt atattcgcgt gagtacacaa
     4381 gaacaagctt tagaaggaca tagtattcaa gcacaagaac aaaatggacg acagtttgcc
     4441 gaacggatgg gctatgaagt ggtagaagtt tatgccgatg aaggaatcag tgggaaatcc
     4501 acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg ctcggaacca tcgttttgat
     4561 ctactagtga tttggaaatt gacccgtctc ggacgcaata tgttagatgt cttacaaaca
     4621 gtcgaggaat tattgcgata tgacgtagga ttacattcga tctcggaaca atttgacgtg
     4681 accacttcga caggtaaatt gatgttacaa ctccttggga gtttcggtga atttgaacgc
     4741 aaccaaattg cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga taaaaagcgt
     4801 tatgcaggag gccgacgttt aggctatgtc agtggtattg acacgaatgg tcaaaaacaa
     4861 ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa atatttaggc
     4921 ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc aaggatatca aacagtcaaa
     4981 aagaatgctt tttctaccac ggcagtcaaa gatatcttac gcaataaaat ttatgcaggg
     5041 tatttagaat atgcgcgcta catcaattgg gaaaccaaac gacggaaggg gaagaatccg
     5101 agaccgattt tagtgaaagg aacccatgaa ccgattattt cggaagaaga ttatcaaaaa
     5161 gtacaagaac gccttgcttt agaaggaaaa caacccaaat ggaaccatac aggtgaaaat
     5221 gtattgacag gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc gagtaatgtc
     5281 acaaataccc taaaggatgg cacaaagaaa cggattcgat attattcttg ttcagtcttt
     5341 cggaataaag gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca agcggaaaca
     5401 tttgtcgccg accgattaaa ggagatcgtt cagctaccag aagttcttcc ccgattggtg
     5461 gcagcgttaa atgaagaaat agtgcgacaa agccaaccat tggaacaaga actcgtggtg
     5521 ttactggaac gaaaggaaga attgaagact aaaattgaaa aatgggaagc tgctttggaa
     5581 gatagtcctg aattgtttcc gatgttaaaa gaccgcttag acgaattgac tgaaaaacga
     5641 cgacagttgc acattcgaga aaatgaaatc ttagggatct ttcaacaagg agagccgatt
     5701 caagtcaaag atgtccaacg gatattgact agtttggatc gttttcttgc ccagagtgag
     5761 aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga aaataacctt tgatcctaac
     5821 cataaggaga tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca acaattgaat
     5881 gagcaatatc aaatagccgt atccaaaaca aaggatacgg ctatttttgt gttaaaaaca
     5941 ccctttacgt taacgatcta gtctaggtta tatcaaacaa cgttagaggg gcgctaacgc
     6001 ttctctagcg atttgaaatc gttagagaag tagttaggga ggtccttatc ataaataggg
     6061 atatttatta atgaatattt gaaatataat tgtatataag aattaaaaag agtggggata
     6121 gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat acactgaact tactaaagaa
     6181 catgtaccac ctaaaaaatc agggaatact ggaggtaaaa agaccagaac tggtaattta
     6241 gatgatttcc ttaagagcga ttttacaaaa ggtgattttc cgaaggggat taaaaggaaa
     6301 cctcaaggaa atgtttatta ta
//
LOCUS       DAZAJZ010000124         6224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000124 DAZAJZ010000000
VERSION     DAZAJZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.128"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG86D_002684"
     CDS             complement(207..1169)
                     /locus_tag="ACG86D_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3141759.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG86D_002685"
     CDS             complement(1171..2610)
                     /locus_tag="ACG86D_002685"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN3141760.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACG86D_002686"
     CDS             2795..4741
                     /locus_tag="ACG86D_002686"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN3141761.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACG86D_002687"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACG86D_002687"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN3141762.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAZAJZ010000125         6200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000125 DAZAJZ010000000
VERSION     DAZAJZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.129"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACG86D_002688"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACG86D_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN3141763.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVTLGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="ACG86D_002689"
     CDS             complement(1378..1896)
                     /locus_tag="ACG86D_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141764.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="ACG86D_002690"
     CDS             complement(1912..2238)
                     /locus_tag="ACG86D_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN3141765.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="ACG86D_002691"
     CDS             complement(2251..4230)
                     /locus_tag="ACG86D_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3141766.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="ACG86D_002692"
     CDS             complement(4251..4565)
                     /locus_tag="ACG86D_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN3141767.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="ACG86D_002693"
     CDS             4733..5026
                     /locus_tag="ACG86D_002693"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN3141768.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6174)
                     /locus_tag="ACG86D_002694"
     CDS             complement(5104..6174)
                     /locus_tag="ACG86D_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3141769.1"
                     /translation="MDFYQSLQLDPFILKQKIREAASKKEKCMYICSLFLRSLFIVLF
                     AICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSIIGLAIVMFSLLVAPY
                     VQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFIVYSLPKDSLNKNFF
                     TQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQEKIWMLIYAFGISLL
                     IVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGSLIGCVLFIGISQFI
                     PFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAVTIRIFENILGVCLG
                     IMYIGVTEILIRKIREKHGLNH"
BASE COUNT         1990 a         1267 c         1003 g         1940 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgtaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt
//
LOCUS       DAZAJZ010000126         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000126 DAZAJZ010000000
VERSION     DAZAJZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.130"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG86D_002695"
     CDS             725..811
                     /locus_tag="ACG86D_002695"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN3141770.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG86D_002696"
     CDS             complement(932..1621)
                     /locus_tag="ACG86D_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141771.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACG86D_002697"
     CDS             complement(1866..2780)
                     /locus_tag="ACG86D_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN3141772.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG86D_002698"
     CDS             complement(2762..3142)
                     /locus_tag="ACG86D_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN3141773.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG86D_002699"
     CDS             complement(3592..3879)
                     /locus_tag="ACG86D_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3141774.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG86D_002700"
     CDS             complement(3899..4123)
                     /locus_tag="ACG86D_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN3141775.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG86D_002701"
     CDS             complement(4578..4748)
                     /locus_tag="ACG86D_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141776.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG86D_002702"
     CDS             complement(4763..5296)
                     /locus_tag="ACG86D_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3141777.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG86D_002703"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG86D_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN3141778.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZAJZ010000127         6172 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000127 DAZAJZ010000000
VERSION     DAZAJZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.131"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(450..1454)
                     /locus_tag="ACG86D_002704"
     CDS             complement(450..1454)
                     /locus_tag="ACG86D_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN3141779.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1525..1821)
                     /locus_tag="ACG86D_002705"
     CDS             complement(1525..1821)
                     /locus_tag="ACG86D_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141780.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1814..2107)
                     /locus_tag="ACG86D_002706"
     CDS             complement(1814..2107)
                     /locus_tag="ACG86D_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFN3141781.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(2253..2804)
                     /locus_tag="ACG86D_002707"
     CDS             complement(2253..2804)
                     /locus_tag="ACG86D_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFN3141782.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2978..3277)
                     /locus_tag="ACG86D_002708"
     CDS             complement(2978..3277)
                     /locus_tag="ACG86D_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFN3141783.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(3304..3612)
                     /locus_tag="ACG86D_002709"
     CDS             complement(3304..3612)
                     /locus_tag="ACG86D_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN3141784.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3729..4688)
                     /locus_tag="ACG86D_002710"
     CDS             complement(3729..4688)
                     /locus_tag="ACG86D_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3141785.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4832..5608
                     /locus_tag="ACG86D_002711"
     CDS             4832..5608
                     /locus_tag="ACG86D_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3141786.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(5706..>6172)
                     /locus_tag="ACG86D_002712"
     CDS             complement(5706..>6172)
                     /locus_tag="ACG86D_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3141787.1"
                     /translation="NSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIKMAI
                     ASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEECLVI
                     EDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT         1880 a         1127 c          911 g         2254 t
ORIGIN      
        1 aataaaagat acgtcaattc acactttatt cttaaaaaaa aactggtctc tctaccggta
       61 atatcgattg agtccgcctt tatcgacttt atcctttttt tatattaaat tttttcaccg
      121 gtagacaggg agagaaagaa taaactaaaa agcccttaat tcttaatgtt ttcacctcct
      181 taccgttcct accgctaaaa tttaatataa ctaaattcta taacattaga agaaatttta
      241 aatacctttg ataaaaaata ttgatgtttt ttctctcatt gtttacaaac tagatagctt
      301 atgtattctt attaaaaata atagatattt cgaacatcat cactctcatc tacttaattt
      361 cgcattttta ctttataaaa aaactgattc taagaaaatt aaataatttt tctcaaaatc
      421 agtttttctt tgataaataa tattttcaat tatttaatca tttgaaaatt gttagtcatt
      481 ccaatacgtt gagaaattct tccatctgta taaacacgga attgttttcc accagccatt
      541 gttgtatttc cattatcatc cgttacaata ttaattgaat agtagcttcc attttcatct
      601 tgcattagat ctttcatata ttcatttgtg ctaattaaat tagtatttga tgcattttta
      661 tcaggagaat catacaacca taaagcttta ttatttagta cagtttcttt agcagcttcc
      721 caacttgaaa ttgctgagct acttgtagat tgtgtcgttg tagttgttgc ttgttgagta
      781 gattcttcta ctactggagt ttgaggagtt tgtccattca caatatcaat gaaaccttgt
      841 tttaactgtt ggttctgagt ttcattgaag tacatgtagt ttttatcatt gccttgattt
      901 tgttgcgtta aataaacttt aggttgtcca ttttgaaagc caaagaaata tacatgctgc
      961 cctaaatatt ctccagtttc tacatcagaa taaactgcta ctaaattgaa atctgcatga
     1021 ccagttccat cttctgacca ttgtacggta actggtgctt cattgattgc cattttccaa
     1081 tttccatcaa taactgcact tggtaaaggc gtaccataca gacttacatt gttctgagga
     1141 ttgtagcttt tatattcttg acctaatgtc tttccccatt gtgggacaaa aattgctagt
     1201 tgagtagatt tctcactatc ccacaaagta tttttaacct cttcagaaga agattcaatg
     1261 attgaacttt cacttgtact tgcttccaca gattgtttca tttctgttgt agtttgttca
     1321 gttgattgcg ttttactttt tttagcctca attgttgtat cagtttctac agtctcttta
     1381 gtcctcgtat tctcagatgt acaaccagtg aataaaatcc ccattccaat tagtaataac
     1441 aaccattttt tcattttatt tccccctttc aatttctccc tcgcgtactt attttatcag
     1501 tcaattaatc tcaatggtgt tcatttagtt aatcctttcc caagaatgtc gatagccagc
     1561 atgacaacga tctcttcctt gagggctttc agatggatta aaactagtag aaatttccct
     1621 tccacatcct ctacagcgat aagttctcat tatttttggg ctattagtat tatttttctt
     1681 agttagagct atgagtccaa ctatgattac cccaactgtc ttaattactt ttcctttatc
     1741 tatattcgga tgcgcaagca tatatttata taaccaatat ccaccgtaaa ttaaaccacc
     1801 taatacaatc agcttagcca tgtactcgtc tcctttcttt ttgtttaatt actttttgaa
     1861 tccatcttgc agagaaacca taaacttctg tcaattgacg gatatctaca gttttttcct
     1921 gttctatcat gttttcgatt ctttttctaa ctaactcacg atcgtacatt ttcattggaa
     1981 aagaaatctg ttgccctcga aaagcaagat aaaattgata gagcaattct gaatccagtt
     2041 ccatttcctc aaaaaagtca ttcatacttt tataagagtt actcaaacaa gtttcatcaa
     2101 tctccatcta cacaccccct gttatttttt acataatgaa aatagcatat tactgggtac
     2161 attgataatt catataacat gtatcatcac atgtattttt aaagtcaaaa agcatgtaaa
     2221 actacttata agtaacataa gaaaataaaa tgttattttt cttgaaataa taattgacta
     2281 tctgccaatt gccattcatt acgaacatga atacttagtg ggtgggataa cttaccttct
     2341 gcatttatca atactttggt catctttttt tgtttcgcac tcaattgatc gtaaatggtc
     2401 ttcgcttgtt cataagccac ttgactcgtc gctgtaatcg aaccacaagc aaaaataatg
     2461 gtgttgattt tcttttctgt gaaaacagtt gttaatattt ccaaagattg ttcatcattt
     2521 ccacgaggaa atacccgagg acttttcgct tttgtatacg aaaaaagatt cacagcaata
     2581 aaaccagtaa atcctaattg tcggatattc ttttcgatta ataacatact caaatcattc
     2641 gtaaatggtt ctgtatctgt tggacgaata gtaatgacta ccgctaattt atcttccgct
     2701 tcttttcctt tgatcgccca ttgtcgtttg aaaacatagc gatgggtatg atgaggatct
     2761 cgccaaactt ctgtttgtgt ggtcactaat tctttttcaa tcatctcttt ttctcctatg
     2821 ctaatttttg ttcaagcaat tcaatcacca ttgccgttat aagtgtcaat actagttgaa
     2881 aatatttcat attctttctc cttttttctt cagaatttga tgaatcgctg taaggatatc
     2941 ttctaaacat ttcgcaatca ttctcaaata atcgatctta ttcattcgtt cctccaatct
     3001 gactccaaac caatcgtcct tctaccagta acccacgaac aaattctcgc tggctggtaa
     3061 tatctgtaat gaaataaact acatagtctt ttccatcttc tggtgcgcaa taaatataaa
     3121 gatgtgtcat cactaaacgt ggtagagtaa catcttcagg aagcataccc atttcaattt
     3181 cctctttttc catcgtaacg gattcatagc cccactcttc aggagtagtg actagctgtg
     3241 cacacttttc cagtacttcg atagccgttt ctaacaagtc ctttcgttcc tttcttttag
     3301 atatcagtat tcttctggta ataacatcgt ttgatactca cccgaatcaa tgacccatat
     3361 tttttgagac actgatttca aacgaagctt aggtgaaatc agatactctt ttcttgcttc
     3421 tggttcttct tgacgatgaa ccaacttttg atttcctttt tccgtagaaa attcaaagat
     3481 ttgaagatag tctaaatgaa attgcttttg aacgttttga tcgattagat tccaacataa
     3541 agcttgtaat tccaccggca aactgtccat tactcccttt gtgacataac gttgtccttt
     3601 attttgaaac atttcttttc cctcctttgc cataaaaata gcccaatctt ctattaaaga
     3661 agattgggct tcatggatat aatatatatt tgatattcaa tttgattcat tttatactat
     3721 ctgatttgtc aaattaagct agacaatatg tctttatcta tgcagctcaa aaatacttcc
     3781 aatggtgttc gataatttaa tgattttctt ggaatatggt ttcttttcga ggcaacggat
     3841 tgaataaagg tttcatccgt gtccctaaag tccattgttt taggtaagcc atctcgtcta
     3901 agcaagccgt ttgagtgctc atttagccct ctttgcgaag gtgttcctgg atctgcgaaa
     3961 aagatggcaa tatcgttcga gttacttata gatcgccagt tggaaaattc ttttccacaa
     4021 tcaaatgtca tggatttaaa caagtgttta ggcatggatt tgaaccaatc atttaacctg
     4081 ttttcgatat cttcggctcg tctgcctgtc ggtttcaaag taataataac cttggataaa
     4141 cactcaacca atgtaataac agcgctcttg tgtttaactc ccacaatggt gtcgacttcg
     4201 agatgaccaa attctttagt caggtgtgga tagagactgt tccgttcatg aatcgttctg
     4261 cgaaaggcct gttttccacg cttttcttga tgattattcg gtttacgttt ccccttcata
     4321 ggtaacgact gtgtgtcaaa aaggcctttt ttaaacatcc gatagagtgt acgaacagaa
     4381 caatcaattg gaaactcgcc acggccgata atcacatcgg gtgaccatcc ttgagctacc
     4441 ttttcttgaa catatgttga ttgttcatca gataaaacgg ttgaacgtcg tccacaacga
     4501 gatttatttt gtttgtattg acggtaataa tctagcactt tttttccttg tttgagaaac
     4561 gaataaacat tataaatggt ttgtctagag cgatttaaac tttgtgcgac aagagcgaca
     4621 ggtttctcga tattgaaata agactctatg attactagtt cgtctgttgt aagatgggtg
     4681 taagtcattt gtactcactc cttattaatt ctttggtcgg aactattttg agtgtagcac
     4741 aaatgatctt tttagttgtc tagcttaatt ttacaatcga cgttttaata aattgtgata
     4801 taatgaagta taacttttta gggagtgtac tatgaatttt caagataata gaccattata
     4861 tttacaagta gaagcttcta tacgtaacga tattcgtgaa aaaaaatatc ttccaggaga
     4921 gcaattacct acagaagaag aattatgtaa aatttatgga gtaagtaaga ttaccattcg
     4981 aaaagctttt aaacttttag cagaaagtgg aacagttgaa cgattaaggg ggaaagggac
     5041 ctttgtaact caaaaaaaag aaagtttaaa attagctgga gttcatggat ttagcgattc
     5101 tctttctacc cgaggacata aaattagata tactgtttta cattctaaaa ttataaaggc
     5161 tgatgagttt ttagctaata aattacaaat caatgtaact gatagagttt acaatattaa
     5221 acgtttaatg tgggaagacg gtgccccgat gggaattgat aattttttcg catcagaaga
     5281 aaaatttcca actttatttg aaaaaggtag ccaatatact tcattatata aacttctaga
     5341 agatgactat gatgtcaaag ctacgagctc tattatggaa ataaacggtt tatctgcaac
     5401 acctgagctt tccgacttat tgcaatgtat agtaggagat ccgctattta ttttagaaaa
     5461 ggttggcaaa gataaaaata atcaaccatt tcattattct caatccacag ttcgttgtga
     5521 tagaattagt tatgttattg aagtcacaga aaatatcact ttagatacgg tgcataaaaa
     5581 tgattcagat cctaaagtaa tagattgata ttaaaagaaa tcatacccaa agcaatcaat
     5641 tttgattaga tttgggcatg atttttattt ttactaatat tctctaaata catcaaaaaa
     5701 cttatttata ttccaaattt cacctgtact tcttgtcgca gatatttaat atcgtaaata
     5761 acatcatctg ctagacttac atcttgagaa aaacgtttat ctttaattgc aagaaccttc
     5821 atatttgctc ttttaccagc ttttatacca ttataagaat cttctatcac cagacattct
     5881 tcaactggaa ttctgagctg ttctgaagct ttaaggaata tagtaggatt aggtttactt
     5941 tctggcaaat ctcttccgct aattaaacaa tcaaaatatg actctatttt acaagtggta
     6001 acaacttcat ttatagtatc cattggactg gaagaagcaa ttgccatttt tatttttttc
     6061 ttttttaacc attcaagctg ttcaattagt atattaggaa aaatatccaa gtaatcaatc
     6121 ttttgcgatt tccaatattt agtaaatttt cccttcaatt ctaaactgtt ac
//
LOCUS       DAZAJZ010000128         6138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000128 DAZAJZ010000000
VERSION     DAZAJZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.132"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..318)
                     /locus_tag="ACG86D_002713"
     CDS             complement(<1..318)
                     /locus_tag="ACG86D_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3141788.1"
                     /translation="MNDFTTEILKTLANKGDLNGLFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIH"
     gene            775..2286
                     /gene="gadC"
                     /locus_tag="ACG86D_002714"
     CDS             775..2286
                     /gene="gadC"
                     /locus_tag="ACG86D_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFN3141789.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            2299..3699
                     /locus_tag="ACG86D_002715"
     CDS             2299..3699
                     /locus_tag="ACG86D_002715"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFN3141790.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3864..5299)
                     /locus_tag="ACG86D_002716"
                     /pseudo
     CDS             complement(3864..5299)
                     /locus_tag="ACG86D_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5692..>6138
                     /locus_tag="ACG86D_002717"
     CDS             5692..>6138
                     /locus_tag="ACG86D_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFN3141791.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         1937 a         1007 c         1174 g         2020 t
ORIGIN      
        1 gtgaatgacg gtctcctcta atgtgtcatt cgtccgtcta taagcaggaa cagtctgttg
       61 cttgaactcg ccattgcggt cgcgcggaat ctggagatga agttccccat actcggtctt
      121 gaccgtacgg tcataggagc cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta
      181 cttttcgtaa tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc
      241 caaatggaca cggaataatc cattcaaatc gcctttgttc gctagagtct ttaggatttc
      301 tgtagtaaaa tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat
      361 tctacagaag ggacttcctt ttttctattt acacaaaata ttttacactc tcaacaaaag
      421 tatcttcgat taatacttgc tcaagttatg tgcagctacg aaaaagtagg taccgaggta
      481 ctttcttttt cgcagctttt tttggactta tgagaagtac attcgctgga agaatacgat
      541 ataaaacgag ctttttcact aaagatatta atagagtgaa agaaacaagc ttttgttgtc
      601 agttttttca cttttctaaa aatgaaaata aaaaattctg gaagtggaga agaattttgc
      661 ttgctatact ttgtcttgta gaaaagaggt agacaaaata atgcaagctc aaataagcga
      721 ttttccataa ccttggtagg aatgacaaat taaataggaa agggaagtaa taatatgaat
      781 aaaaaaaagt tatctctatt cggttttttt gccctgacag cttccatggt tttgactgtt
      841 tatgagtatc cgacctttgc aacatcacaa ttacatttgg ttttctttct tcttttggga
      901 ggtattttat ggtttctacc agttgcttta tgtgctgcag aaatggcaac agttgaagga
      961 tggaaagatg gtgggatttt cagttgggtt agtcaaactt taggagaacg ttttggtttt
     1021 gcagctattt ttttccaatg gtttcaaatt acagtaggtt ttattacaat gatctacttc
     1081 atactaggag cgctgtcgta tgtaattaat tttccagagc ttaatacaga tccatttatc
     1141 aagtttattg gtgtattagt cattttctgg gtactgactt tttcacagtt gggcgggacc
     1201 aatcgtaccg ctaaaattgc taaagcagga tttgtcattg gaattgttat tccttcgatc
     1261 attctctttg gtttagcagc agcctacttt atcggcggta atccgattca aatggctatt
     1321 aatgcgaaag cttttattcc tgacttttct cagctttcaa ctttagtagt atttgtatct
     1381 tttatattgg cttatatggg cgtagaagct tcagcatcac atattaatga gctggaaaat
     1441 ccccaaagaa actacccatt ggcaatgttt ttactagtga ttttggccat cgccttagat
     1501 gcaatcggcg gtttctcagt agcagcggtt attccacaac atgacttgtc tctcagtgct
     1561 ggtgtaatcc aaacatttga aacgcttatt ttacatttta acagtcattt acattggttg
     1621 gtaaaagtga ttgctctcat gattgcctgt ggcgttatgg gtgaagttag ttcttgggta
     1681 gttggtccat cacgagggat gtttgcagct gctcagcgag gtctgttacc agttttcttc
     1741 cggaaaacta ataaacaaga cgttccagtt ccgttgatta tgatacaagg aattatcgtt
     1801 actatttggg cagcagtctt aacttttggt ggaggaggca acaatttatc attcttagta
     1861 gcaatctcct taactgttgt catttatctc gtaggatatt tactattttt cctaggttac
     1921 tttgtattag tttttaagaa aaaagactta aaacgaactt acaatgtacc aggaaaaact
     1981 gttggcaaaa cgattatcgg tggaattggt tttattttat cagtgttcgc attgcttatc
     2041 tcttttgtac caccagcttc aattgcagct agtgagcacc gaacatacca aatcatacta
     2101 cttattagct ttgtagtgac tgttctttta ccttttatca tttatgaagt acatgataaa
     2161 agtgctcaca caactattga ggaacctact cacgtgaaag ctgaagacgt taatcccgct
     2221 gtttatccag ctgctagagg agagcaccac gttacaaaaa atgatgaaca tattttaaaa
     2281 cattaggagg aattcattat gttatacgga aaagataatc aagaagaaaa aaactatttg
     2341 gaaccaattt ttggctctgc aagtgaggat gttgacttgc ctaaatataa gttaaacaaa
     2401 gaatccattg aaccacgaat tgcttatcaa ttagtacaag acgagatgtt ggatgaagga
     2461 aatgcgcgat taaacttagc tactttttgt caaacgtata tggaacctga agcagtgaaa
     2521 ttgatgaccc aaacgttaga aaaaaatgca attgataaat cagaataccc acgaacaacg
     2581 gaaattgaaa accgctgtgt aaatatgatt gctgatttat ggcatgctcc aaataatgaa
     2641 aaattcatgg gaacttcaac gatcggctct tcagaagcct gcatgctggg tggtatggcc
     2701 atgaaatttg cttggcgtaa acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa
     2761 cctaacctgg tgatctcttc tggttaccaa gtttgttggg aaaaattctg tgtatattgg
     2821 gatgtggaac tgagagaagt cccaatggat gaaaaacata tgtcaattaa tctagatact
     2881 gtcatggatt atgtggatga gtacacaatt ggtattgtag gtattatggg tattacttac
     2941 actggtcgtt atgatgatat caagggtctg aatgatttag ttgaagctca caataaacaa
     3001 actgactata aagtatacat tcatgttgac gctgcatcgg gtggctttta tgcaccattt
     3061 actgaacctg atctagtttg ggattttcaa ttgaaaaatg ttatctcaat taattcttca
     3121 ggtcacaaat atggtttggt atatccaggt gtgggttggg tcttatggcg tgaccaacaa
     3181 tacttaccag aagaattagt atttaaagtt agttacttag gtggagaaat gccaactatg
     3241 gctatcaact tctctcatag tgcagcacaa ctaattgggc aatactacaa ctttgtacgc
     3301 tatggctttg atggttatcg tgatattcac caaagaactc atgatgttgc tgtttattta
     3361 gccaaagaga tcgaaaaaac tggtattttt gaaatcatta atgatggatc agaattgcca
     3421 gttgtgtgct ataagctgaa agaagatccc aatcgcgaat ggacactata tgatttatct
     3481 gatcgtctgt taatgaaggg atggcaagtc ccagcctacc cattacctaa agacttggat
     3541 caattaatta ttcaacgctt agttgttcga gcagactttg gaatgaacat ggctggtgat
     3601 tatgtacaag atatgaacca agcaattgaa gagttgaata aagctcatat tgtttatcat
     3661 aaaaaacagg atgtgaaaac gtacggcttt actcactaaa attcttcaga gaaatataaa
     3721 agaagttaat taactaataa aaggagttgt gattaccaca cagctcctta aatctatttg
     3781 aataaatata tgatattcat ttgttacact ctcaatctta gctgttgttc tattcatcat
     3841 tactcttatt gtaagtagtt cagtcaaatt tgcttgttca attgatcgat aacagtatga
     3901 attgtttgaa aagtaatcgg atatataatt gttttctcac tacctaagcc ttcttggtat
     3961 aaagaactat cgcaaagtat aatccgctga gtacaaaaga aagtgtcgtc tactatttct
     4021 ctaagttgtt tacaaatgat attactaata gaaaagtgtt ctcccaaaat gtcttctaac
     4081 tgttgtcgat agtataaata cctaaagtct gacggagcta ctatgaacac ttcaattgga
     4141 gatttccgtt tatgctcatt taaaagactt aaactaatgg ttagtttacg aatagaattc
     4201 tcattccaac gcagaaataa attgttttgt tttcgccatt gttcaagaat ttcatgaact
     4261 tctaagtaca aagcgcgttg ctcttcattt aacaagtaag tcttatcagg ttgaaatatc
     4321 tgaatatcag cccaaagtga gcggataaac gggagaagtg ctttatcaaa aagtatctga
     4381 ttttcttcag atatattcag agataagata agtttttgac tgagctcaat caccaaaggg
     4441 ttatctgcta catggttttg ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta
     4501 tcgcatagat aatttctaga gttgaataga aagtagatat aaaggcattc atcttcgtca
     4561 tgacataaag agaaagaaag ttgctctaat cctttctgta ttagctgaaa caagggagta
     4621 tgtcgggcaa ctcttttttc ttcctgacta aaatatattg ggttagtctt agatctctgc
     4681 aaaccgatag cgatacctaa taaaataaat tgtttttcaa ctgtagggta acttcttgaa
     4741 aaaaatgttt ttctacataa tcaatcaatt gttgaccgct ttgaataatt gatttattta
     4801 gctggatcaa ctctgtccga tttgtctcat agattatcgt tagtaacaaa cttcgataat
     4861 ccttttcagg aatttcaaaa tggccatcag catttaagta ctgcatttgt ttaaaaaaat
     4921 taatcaactc atttcgtaag ttgtaaactt tgcttaacga aatgaaggta gtatcagcta
     4981 tattactcca attgtgttcc cctgataaat aatgtcctag tacattgaaa aagtcagagc
     5041 gctgatagat gagttgagtg agtgccaacc tcgaaaacga agagtcgaat gcgactgtaa
     5101 agctacttct agatttctcc actgaaataa tttgctgatg gagtatgtcg gtaatgcatt
     5161 ccaagtcgtt agtgacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     5221 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     5281 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     5341 taccaatcgg aatagtcctg taatatattg aatcaaaaat ataaattata aaataattag
     5401 atggggtatt tggtttaagt atcccttata tagtaataaa tttataggtg tgattctcga
     5461 attaataccc gaaaatgtga gagaattttt atggattttt taggatacaa attaattatt
     5521 tttgctttta tcctgattac cagtccagta ctgtcggtat cccattttgc agcaaaatca
     5581 tgttgataaa gtgacaagaa gactgatgag atatattatc caaattgaga agataattaa
     5641 tcggattctt gagtataccc tactttttaa gaaagtaggg tatacaagcg tatgaataaa
     5701 gacataactt tccctgtgat ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac
     5761 gattataatc ccaatcgagt agcgtctccg gaaatgaact tattggagct ttctatcatt
     5821 gaagatggct ttactcaacc attagtttgt tattacaagg aaaaagaaga gaagtatatc
     5881 ttggttgatg tgcttcatag gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta
     5941 ccggtaacag ttattgataa acccatagaa gaacgtatgg cttcaacaat tcgacacaat
     6001 cgtgcaagag ggacccatga aataagagga atggaaattg tcatccaaag attagtaaaa
     6061 aatggatggt cagacaagag aatatccaaa gaattaggca tggacattga agaaattttt
     6121 cgctttaagc aagtgtct
//
LOCUS       DAZAJZ010000129         6136 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000129 DAZAJZ010000000
VERSION     DAZAJZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.133"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..764
                     /locus_tag="ACG86D_002718"
     CDS             156..764
                     /locus_tag="ACG86D_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN3141792.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            764..1105
                     /locus_tag="ACG86D_002719"
     CDS             764..1105
                     /locus_tag="ACG86D_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141793.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            1347..4136
                     /locus_tag="ACG86D_002720"
                     /pseudo
     CDS             1347..4136
                     /locus_tag="ACG86D_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            4126..4866
                     /locus_tag="ACG86D_002721"
     CDS             4126..4866
                     /locus_tag="ACG86D_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3141794.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            4863..>6136
                     /locus_tag="ACG86D_002722"
     CDS             4863..>6136
                     /locus_tag="ACG86D_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN3141795.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNG"
BASE COUNT         2217 a          775 c         1229 g         1915 t
ORIGIN      
        1 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
       61 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
      121 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
      181 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaattttc
      241 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
      301 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
      361 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
      421 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
      481 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
      541 gacagtttgc tctagattca gatgaaacat caacgattga aggaagtaaa gtaagtttta
      601 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
      661 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
      721 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
      781 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
      841 tgctgaattt ctaccaggcc cagtcgtaga agaagcagca gaagtaatgg agggaatgca
      901 aaatgctgta gatggaaaat cagtatcaga agcattaagt cgagcttatt catttattgc
      961 agatattttg tttgaaggac agtttactgg tgaagattat cgtcaaggga tcgatgctcg
     1021 agaaattgct tcgttgacag gtaagttatt aaaatctgtt actgcaggct ttgatgaaac
     1081 ttatacagaa acgaaaaaaa agtaagcgaa gctctcaaat atcctttttt taaacattcg
     1141 attacttatc gagaattaga tttaaagacg caattactcg aagcaggttg gacgttacca
     1201 gaaattgaac gtactgactt tgacgagtta atgcgtcttt ttgcttttcg agatgcagta
     1261 aaagagcatg aggatgttga gtactacgat aacttcaccc aattttagga ggtgatactt
     1321 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
     1381 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
     1441 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
     1501 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
     1561 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
     1621 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
     1681 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
     1741 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
     1801 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
     1861 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
     1921 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggttta tctcaaggta
     1981 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
     2041 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
     2101 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
     2161 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
     2221 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
     2281 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
     2341 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
     2401 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
     2461 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
     2521 tagctgtttt cggttacttt atggcaacca atgaagagct tagaaacaag gttatatcta
     2581 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
     2641 ttgatacttg gaattctgct aaataaatgg catcaaatgc agttgagggt gtcaaagatg
     2701 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
     2761 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
     2821 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
     2881 gagaaaaatg gaatggaatc aaaggttcta ttatgtcagt tgctggtcca ttgattacag
     2941 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
     3001 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
     3061 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
     3121 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
     3181 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
     3241 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
     3301 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
     3361 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
     3421 ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg gcatacaata
     3481 cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag gactctttct
     3541 ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat ctaaaaaatg
     3601 gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg gaagtcaatt
     3661 tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa gaaaagtgga
     3721 atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg attaaaggag
     3781 ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa aatattgttc
     3841 aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc atgacaaatt
     3901 tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta ccaattacac
     3961 aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat ttaaatgttt
     4021 atggagattt acctgattca atggttcgaa agattactac aaaaattaaa acagaattga
     4081 caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca acttaagaga
     4141 ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat tcaaaacagg
     4201 cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg aaatgattct
     4261 atcattattg ataaaggcta ttacaaaaat gtgactagaa agattgagtg ttactataaa
     4321 gcaccttcaa ttgatatggt gcaggagtgg gaagatcgaa tcactgaatg gttagatatg
     4381 tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca agctgtagta
     4441 atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc ttttgaattt
     4501 acagtgagta ttcgaccatt taaggaaaat tacaatggta gatttactat tcgacaaaca
     4561 gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat taaattgagt
     4621 ggttctggag atgcttcttt ttatattaat aaggataaat atgatttgaa atcgttggac
     4681 agagaattat ttatagattc taaattagaa gaggcttatc gaaaattaga tggtaattta
     4741 gaacatcagg atcaggtcac cttattttta gattttccat ttttatatcc tggaaaaaat
     4801 gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa
     4861 gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa
     4921 ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag
     4981 agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa
     5041 aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag
     5101 atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac
     5161 gggtagtcac tcttgtggaa gtggactcta aaactggtca agaagcgata tcaattattg
     5221 aaactaaaat ggataaaaaa tctgacatac gactttattc tgatattacg gctgtttcta
     5281 gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc
     5341 ttcaatattg gggcggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa
     5401 gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt ttgaaggtca
     5461 aattagattg gacaggtata aaaacaagaa ttattccata tgctgatcct caaagtgaag
     5521 taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc
     5581 ctgatgtcta tacagaacat gttcagttta cagaagaaca aggagtaaaa gacattaaga
     5641 gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta
     5701 aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg
     5761 cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata
     5821 tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga
     5881 aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta actattcagg
     5941 ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt
     6001 caatgattac aggtcaaggc agtgcaggag gatatgtagt tttatggcca aaagaaaaac
     6061 cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa
     6121 tgaataaaaa tgggat
//
LOCUS       DAZAJZ010000130         6092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000130 DAZAJZ010000000
VERSION     DAZAJZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.134"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..547
                     /locus_tag="ACG86D_002723"
     CDS             191..547
                     /locus_tag="ACG86D_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3141796.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            557..1456
                     /locus_tag="ACG86D_002724"
     CDS             557..1456
                     /locus_tag="ACG86D_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3141797.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1976..2704
                     /locus_tag="ACG86D_002725"
     CDS             1976..2704
                     /locus_tag="ACG86D_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3141798.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2849..4876
                     /locus_tag="ACG86D_002726"
     CDS             2849..4876
                     /locus_tag="ACG86D_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN3141799.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            4984..6081
                     /locus_tag="ACG86D_002727"
     CDS             4984..6081
                     /locus_tag="ACG86D_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN3141800.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2110 a         1007 c         1260 g         1715 t
ORIGIN      
        1 caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt
       61 ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa
      121 cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga
      181 ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt
      241 agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag
      301 ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca
      361 aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa
      421 agacagtgca atactttaca tggatgatcc atccacactt gctttagaac caacttataa
      481 tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa
      541 agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa
      601 taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac
      661 tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga
      721 atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga
      781 tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta
      841 tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt
      901 ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg
      961 gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa
     1021 aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt
     1081 tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa
     1141 tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca
     1201 agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta
     1261 cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa
     1321 agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca
     1381 cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac
     1441 aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata
     1501 aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat
     1561 aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg
     1621 tgtattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt tttatttaat
     1681 tataattaat attgattgaa gtctaggcag tcaagtcaca ttttgataga taggaggggg
     1741 acttacgaat tattttaaag gctatgacag ctgtatttct agattgtgtt tgcatcaagt
     1801 tgcaataaaa actagaaatc aaatcgggct gataatcatt tataactaat cgatacatag
     1861 gaccaaatat aggtctatgt tcttttattt cataagtatc gtaaaacaga attttgctat
     1921 ggattaatcc aatcgactga tatgtttagt aaataaagaa agaggaatgt caatgatgaa
     1981 aaaagaaatc gtattaacag cagcaatgat gttattcagc atggtagcaa gtacgacttt
     2041 tgtttctgca actgaagtat acccaaagga atataacaca gaaggaacga ttacatttga
     2101 agcaggagat gagggtgtaa ctcctcctgt tgatccagag aatcctgatc ccaataaacc
     2161 tgtcgatcca tcagatcctc caagccctgg aacaggaggg gcattatcga ttgattatgg
     2221 atcaaaattc aaatttggga ctcaaaaaat ttctactgcg gataaaacgt attatgcagc
     2281 tgctgatgtg atgaacgatg ggtctagaaa accgacatat gtccaagtga cagataggag
     2341 aagtacctta tctgggtgga aattaagtgt ctctcaacca gaacagttta aaactgccag
     2401 tggggatgaa ctagttggag cacagctgaa atttacgaaa gggcaggctg tttctttggt
     2461 tgatccaaca tatacgccgc aaacggtgaa ttctgaatta acgttgacac cgggaggaaa
     2521 caatacgtta gcaattaatg ctaaatcagg aacaggtgtg gggacttggg tctatcgatt
     2581 tggtgcaaat gaaaatgaaa atcaagatgc ggtacagctt tcggtacccg gtaaatctgt
     2641 caagttagcg caacaatatt ccactaaatt agtttggaca ttagaagata cacctaacaa
     2701 ctagtttact gaaaagaact gttaaaaata aatacgtagt catattgtct tgcgaggtaa
     2761 tcgagcaaaa attgtcaaag gctaggctaa atttgtttgg atacgaatag ttcatctagg
     2821 aatttaacga tcaggaggaa aagatatatt gaaaaagagg aggatcgtgc tcattttaag
     2881 cagtctctta gtgactaata tctttccgat catagggtac tctttggaat tgaatcagtt
     2941 ggagcatgga aatagaagag acgaagaaag caaactcact tcgacattcg acagtttaat
     3001 agaatccaac cagacacaag aaagcagtga ggatttaagt atttcgagta gtggagagac
     3061 cactgacaac gtagaagctg agggttcgga caaacaagga ctagaagatg caccaatttc
     3121 tgcggttgag ctgcctgacg atcccaatat tatttctatc gataaaattt ttcaaacacc
     3181 tatcggtact agcacgtcca tattggaaaa tgggaagtta ctgcagttga acccagcaga
     3241 aaggtctcaa agaggagcta tttggtcaaa taagaagatt agtttactct cggattttac
     3301 atttaaatct tatttatact taggaaatca acgagataga gcgggagatg gcatgacctt
     3361 tacattgact ggcgatccta gaatggctag taatcccagt caagttattg gatcgccagg
     3421 tatgggaatt ggctcatatt ctactcgttc tggccaacct tacattgcga atgcgctctc
     3481 gattgagttt gatacgtata aaaatagtgg ttcttctgat cgaatggatc gagagataac
     3541 tgctgataat agagggcatg gacatgtcgc ttttgtcacg cctaaaccaa ataataataa
     3601 ttattctgga gagcatactg gcgtgacggt agctcctaca tttctttcta atggcacatg
     3661 gcgcttgtta ataattaaat gggacgcgga gcaaagaagt cttacttatg atttggaagg
     3721 tgtcggtcgc tcttctcgga ttatacctga tctcattagg cagtttggtt ctacagaagt
     3781 aaattggggg tttacttctt caacaggtac tttttttcaa gaaaatgctg tcgctgtcac
     3841 gcgacttcca tcgaatgtca catcaacagc tgatgtaaaa gtcaacgata gtgattatgg
     3901 ggccatcgct gaagctggag gaaacgataa gataactatc agaaatacat tgaaggtcga
     3961 gggggatttt cttgaatata agaaaatgca gactagtatc gacctcccac aagaattaac
     4021 tattcccgag catatcttga tcaatggaaa tcaaattgat gccaaagata tcttgataac
     4081 cggaaatcga gtcacgctaa atctgacaga ttattttatt gctggagata cgttaataat
     4141 cgaattggag acaactttgc aggaagtaat tcctgaaaaa gtactcacta tgcattttga
     4201 ataccttgaa aacgggaatt tgattgagca atcgaatgaa ataaacctaa agatcgcaaa
     4261 gctgaaggag atagatatcc atgtttatta taaagataaa gacagtcaga ttgatattgc
     4321 tgaaatgaaa acaatcactg ggaaaatagg agagaagtat aaagaaactc ctattgatat
     4381 tgaagggtat gtttttgact cggattcagc gaatacagaa ggcatcttct ccgaaaatac
     4441 agaggatatc tatttttatt acagaaaagg caaactttat ctctcaactg ctcctcaata
     4501 tgtcaatttt ggaaaacata aaatatccag tagaccatta agtgaattcg gacagttttc
     4561 aggtgggttg aaaattatag atgaacgggc ttttggaagt tggaaattac aattgaaaca
     4621 agagaaagtt cttacaaatg gagattttga gcttccagaa gctctttcct ttgttacctc
     4681 tgaaaataca acggttatag ggacatccgc tgttacgatt ttcgaaagtg atcaaaaggg
     4741 tgaaagcgat ttgtcctctc ttttagattc tacagctcat cgtggaatca gaataaatat
     4801 tcctgtagaa aaccaaagaa tcggaacctt tgaggggaaa ctatcttgga tacttgcaga
     4861 tgtcccagga aattaacagg aagagaagaa tataacaaaa ttattgagtt tcaagaaggg
     4921 cacaggtacc ataggctctt gtgtcccaca gagacaagat tttgttatag tcttctctta
     4981 catatgatta aaggagcgat tttcttgaga ccaaaacaac aattatttat aacactccta
     5041 gttattttag gggcactttt taacaatata tcagtgtcag ctagtgcatt taatttttca
     5101 gtgacgccga taacttcaga gaatcaaatc gacaagagaa aaacttactt cgatcttcaa
     5161 cttgatccgg atcaggaagt agaagtaaaa gcggaattga gaaatgatac agaaaaagaa
     5221 gtgaaaatcg atatttctgt gaatagcgca accaccaatt caaatgtgat ggttgaatac
     5281 ggaaaaaacg agattgaaaa agacatgagc ctgatctttg atctggtaga ttatgtgagc
     5341 tatccacaga tagtgacgct aaaacctaaa agtgtccaaa ccgttgcttt tcatgtcagg
     5401 atgccgaatg agcgatttga tggtgtattg gctggtggga tcacattcca agaaatccag
     5461 acggaaaagg accaaacaga aactaaggat caaagcttat cgattgagaa tgaatacacc
     5521 tatattattg ctttgttgat gcaacagaca ctgaacgaag ttgctccgaa ccttactctt
     5581 catgaagtta aaccagatca aatcaatgca agaaatgtca tcttagcaaa cgttcaaaat
     5641 gatcaaaaaa cctatatcaa tcaagtagtt attgaaacaa aaattacaaa aaaaggacat
     5701 tctgaagttt tatatcaaga ggaaaaagaa ggattgcaga ttgcgccgaa tacaaatttt
     5761 tcttttccaa ctgctttaaa tggtcaaccg ctaaccccag gtgaatatca ccttacaatg
     5821 actgttttag gaaatgaaaa tgaaaatgga aaattttcga gaaaaaaagg aaatactacg
     5881 atcaactata ccaatcaatg ggtatttgaa aaagatttta caatagacgg aaaagtagcc
     5941 aaggaattga acacgacgga tgttacgatt aaaaaagaca ataattggat ttatatctta
     6001 gtagcttcac ttttcttatt gtttatttta ttatgcatac ttttgtttgt tttgagaaaa
     6061 aaagaagaga aagaaaagta aggttaagat aa
//
LOCUS       DAZAJZ010000131         6069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000131 DAZAJZ010000000
VERSION     DAZAJZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.135"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACG86D_002728"
     CDS             complement(<1..229)
                     /locus_tag="ACG86D_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3141801.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
     gene            548..1273
                     /locus_tag="ACG86D_002729"
     CDS             548..1273
                     /locus_tag="ACG86D_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141802.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            1263..1772
                     /locus_tag="ACG86D_002730"
     CDS             1263..1772
                     /locus_tag="ACG86D_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141803.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1842..3290)
                     /locus_tag="ACG86D_002731"
     CDS             complement(1842..3290)
                     /locus_tag="ACG86D_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3141804.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3290..4006)
                     /locus_tag="ACG86D_002732"
     CDS             complement(3290..4006)
                     /locus_tag="ACG86D_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN3141805.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3987..4337)
                     /locus_tag="ACG86D_002733"
     CDS             complement(3987..4337)
                     /locus_tag="ACG86D_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN3141806.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4481..5254
                     /locus_tag="ACG86D_002734"
     CDS             4481..5254
                     /locus_tag="ACG86D_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3141807.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5425..5791
                     /gene="ssrA"
                     /locus_tag="ACG86D_002735"
     tmRNA           5425..5791
                     /gene="ssrA"
                     /locus_tag="ACG86D_002735"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5837..>6069)
                     /locus_tag="ACG86D_002736"
     CDS             complement(5837..>6069)
                     /locus_tag="ACG86D_002736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141808.1"
                     /translation="FEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLG
                     ISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1773 a         1187 c         1057 g         2052 t
ORIGIN      
        1 cagacacatc attcagtaac aaccgaacgg ttgtcgtccc ccaacgaatg atcctagagg
       61 ggacgacaca aagacaatgg gaacactctt ctggcttgta cagaagagtt cctttgtaaa
      121 tcaagcaaat ttgccctttg tatttttctt tagttaaaca attttcctcg aaatagatat
      181 ttaggtctag aatatttagg gtatctttga taagctgagt atgagacatc ttgaatcctc
      241 ctatgaattg gtagccaaat taattctaaa ggatttcagg gtgtcttttc tatatttttt
      301 tatacaaaaa agatgttggt gaagttcacc aacatcaaaa agtatagagc ccaaaattat
      361 tgtcgctgct cctcttttcg aataaacagt gttcaaaagc ttgcatctac ggtaatagaa
      421 tagggcagct tcttcacaac ctcttgcttt ttacaaaata ttttctggtt ttaattcgtg
      481 atttcactaa aggaatttca tactttattc atattcattt tctatacttt ttatagaggt
      541 gaaaagtatg aaaataatcc gctatgctac tgctcgatta atttattaca aaaagcagac
      601 aatactctat tgtttatttt caagcttttc tgctttttta ttgatagcat gcctgactct
      661 ttaccattcg caatcggctt tagccaatca agtccaagat cggctttcat ttattggcgc
      721 ttctgcatct acagagcttc ttccggcttt agaattagct agccgatttt atataaaagg
      781 catattcagt ttattttttc tatttgcctt cctttttatc tttttctttt actattcagt
      841 caggcaaaac aagcaggaat tgatcaattg gcgcctgact ggtttttcta aaaccaaatt
      901 attccttttt attttctggc agctgttctt tcctctcttt ctttgttgta tcttggtttt
      961 attgtggatc gtcagcttcc agtcagttta tgaagcgctt cttcaaagga tcaattttct
     1021 tgtcttaaag actactgaac tgccagatat ccgtgatatc ttgacttcgg taagcggact
     1081 tacaattccg atggatcaac agactttttt taccatcgat ttccaaaatg atcttttttt
     1141 cacagatata cttaagggat ttgcccaaac tcttattggg ctcagtggat gctgtctcat
     1201 tcttagtcta ttccctttca ctttttttat ccatcatttg caaagtagga ggcctggacg
     1261 taatgaaacc tgaatatttg attcatgctg aacacttgac cgttattttg tgtcctctcg
     1321 aaaaacagca taaaatattg gagcacttga aaaaagaggt tcttgttgca aaactggata
     1381 attttgcgat catccaaaaa aattggccga tgtttcctta tctgaatctt aaagaccatg
     1441 tcctgttgga tgtaccggaa aaagaaatca aacaagatcg tttggcctat caagaaaaac
     1501 ttgatatctc tccttccttg cttaattgtg cggcagctga gttgacgtct tttgaaaaag
     1561 taaagctgca attacttcat gccttgcttt ccaagcgaaa caatattgtt attgaagata
     1621 catttgatga acttagtgta ttggagatcc aagagctact ccacttgctt tcttatttag
     1681 ctcatgaaga aaatcaaggt atcttactct ttactcatga tgctactatt gctcaaagtc
     1741 cttatattga tcgtttagag cccgcgggct aaatcaatca ttttatagta aaaacaagcg
     1801 gtatagaaga tcgattcatg atcttttata ccgcttgttt tttactcttt tttgatttct
     1861 acgtcaggca aacgaacagc gaattttgtt ccttttccta gttcgctttc tacttctaat
     1921 tttcctttat gcaagcgtac aagttgctgc acgattggta aacctaatcc tgattcacca
     1981 tattttgtat tttttcttga aggatctact ttataataac gatcccagat acttttcatt
     2041 tgttgttcag acattccaat tcccgtatct tcaatcgtga tgatcgtttc aagatagcct
     2101 ttttctaaag taatcgtgat ttggccgttt tcagtaaact gtatcgcgtt ttgaataata
     2161 ttgaccatga tttggacaaa tcggtcataa tcagcgtaaa catcaatatc atcaacggct
     2221 ttcaatgtca atgtatctcc tgctgcttct gctttagctt ctaactgtgc aacaatattt
     2281 ttcaatgttt ctgttccatt gaatttttta atgaccatac tgatttgatt cgtacggatt
     2341 ttttcataat ccaagttttc attcaccaaa cggatcagac gctctgtttc atttttcatc
     2401 aatttaatcg cgttttctcg ctgattttca gggattgcat tgtattctaa cccttccaga
     2461 agtccgttga ttgtcgtaag aggtgttctc atctcgtgag aagcatctgc cataaattgt
     2521 cttcgacgat tttcttgttc ttcaatttct gcttgagatt ctttcaatga attcgtcatt
     2581 ttattgaaat catctgctaa ctcatcaaat tcatctttgt catgtacggg gagctgtaca
     2641 tcaaaattac cattcgtcac ttctttcgtc gcattcctta accgattgat acgcttaact
     2701 tggaatgctg caaaagcata gctggcaata actgcaacga tactggaaaa aataaatcct
     2761 ttgaacaggt tcagtgtaat tggacgaacg ctgttatcga tatttcgcgc aggttgacta
     2821 actacaagtg ctccatagaa ttcatgattc aaattaaatg gcacaagtgc gtaagaggtc
     2881 gcctgtgctt gtcctaaaat atttttattc gatgtgaatt tttcttgacg tccattcctc
     2941 agatttttcc attgatcagc tgttaaggta aaattcagta aagctgtatt agtcggataa
     3001 atgacacgtt cgtttttatc gataaatata aagttcacat tttgttctgt cagtacttgc
     3061 tctgtcagca gcaatgcatt ttgaaacgcc tgatcttgac taaactgcgg gagcgtatcg
     3121 gcataggttt gcgttgtctt ttcaactgat tcagcatatc caaacagttg ctggtaattg
     3181 ttttcttcaa tcgtttgttt tgtcagttgt gtaaaagaaa ctccaacgat caataagatc
     3241 aacgcgacca ttccgcaaaa agctaagagt tgctgataca aataacgcat taggcaacac
     3301 cagaatcatc gaatttgtag ccgactcccc aaacagtttg gatgacttgt ggtccgacct
     3361 tttcgatttt ttgtcgtaat ttcttgatat gggcgtccac tgtccgttca tcaccaaagt
     3421 actgataatc ccagactaat tcaagcaact gctcccgaga gaatacttga cgtggttttt
     3481 ttgctaaagt aaacaataga tcgaactctt taggcgtcag tccttcaatt ggccggttgt
     3541 ccagatacgc ttctcgtgtt ttcgtattca tcttgaaatg actagtctcg atatcaaaat
     3601 gatcatctga aactgctgct tcttctcttg cttctgccaa ctcactacgg cgatgcaaag
     3661 ctttcattcg agcaataagc gtcagaggac tgaaaggttt tgtgacataa tcatctgccc
     3721 ccatctctaa gccgatcacc tgatcgcttt cagaatctct agctgtcagc atgataatcg
     3781 gcacagtact tgaaactttg cggatctctc ttcctactag cataccgtcc agtgtgggca
     3841 aattcaggtc tagtgtaatc atatcccaat gttgtgggtt ttctaaaaag gcatcgagcc
     3901 cttcttttcc atcatacttg aaagttgctt cccaaccttc atttaagaaa aacatctgca
     3961 tcatttcaga aacagattga ttatcttcaa tcattaaaat attcattatc gttccctcca
     4021 gtttccaggt catatttctg taatttcttt tctgtcataa taaaaatagg cataattgga
     4081 aatactagca gaagcatttc aatcagaaaa taattcgtat gcccagatgt ctttaaataa
     4141 tagccgatca atgccatcag taagcccata agaaaaaacc agtttccgct tgtcttttga
     4201 gccagccgcc aatgagcgtc tgtttttgcg gcaagaggcg aatgataccc ataaaaaggc
     4261 agatttcctt ttgagggcag aatccgaaaa agactgccaa ttattaagaa taagatacca
     4321 atactgaaaa aaatcattct ttccctccta aattctcttc ttatagtata ctctaaattg
     4381 ttgactgaac caatatgttc aggaattctt cacgaaaggt ttgtcttttg ttcattcttt
     4441 gttaagataa acttattcca ataaaaaagg agttttattt gtgtcaaatt atcgtgcaat
     4501 cactttcttt gatcttgatg gaacattact agatgcgaaa tcaaaaatca cacccgaagt
     4561 ggcaaaagct atgaacgcat taaaagaaaa caatgttctc cccgtcatcg ctacaggacg
     4621 tacagaagca gaaatccact ctatcatgga agatgcgggc attacttctg cggtcaccat
     4681 gaatggttct ttcattcgag tagacggcga agaaatctat tctcaaacat tttcttcaga
     4741 agaatgtcaa aaaatgtatg atcatgtaca gttgaacggt catgaacttt cttactataa
     4801 cgatcagaaa atctggtgta ccggtcatag ccaaacaatg atcaatgctt acgacttcat
     4861 tcattcagat gttccggaaa ttgatccttt aggctttaaa gaaaacacag taaatatgtt
     4921 gctgatctta tctcaatcag gtgacgaata ctatcacgaa cattttcctg agctaacttt
     4981 ttatcggaat ggtccttact cgatcgatac agtgaagaaa ggaatctcaa aaggggcagg
     5041 tgtcaaacgt ctgcaaaaag aacttcagct ggaaaaagtt cctacttttg gatttggcga
     5101 tggtccgaat gattttgcct tgttggaagc ttgcgacaac aaaatcgcta tggacaacgc
     5161 ctatgacgga ttaaaagaac tttctacctt tatcacaaag aaaaatacag aaggcggaat
     5221 tgtccacgca ttgaagtatt ttgatctgat ttaagatctt tttttgaagc tacctctttt
     5281 cttaagaaaa tcgcttattt tttcttaaga aaggggcagt tattatataa ataatgggat
     5341 atgattcgct tttttattga atttatccca atatatgcta tactaatttt gtatgaaaaa
     5401 tacacgtaac ccttgttagc taacggggac gttacggatt cgacaggcac agtcgagctt
     5461 gaattgcgtt tcgtaggtta cgtctacgta aaaacgttac agttaaatat aactgctaaa
     5521 aacgaaaaca actcttacgc tttagctgcc taaaaacagt tagcgtagat cctctcggca
     5581 tcgcccatgt gctcgagtaa gggtcctaac tttagtggga tacgtttcaa ctttccgtct
     5641 gtaagttgaa aaagagaaca tcagactagc gatacagaat gcctgtcact cggcaagctg
     5701 taaagtgaat ccttaaatga gttgactatg aacgtagatt tttaagtggc gatgtgtttg
     5761 gacgcgggtt cgactcccgc cgtctccata tatgtatggt tgagacagat aaacatgtat
     5821 gaaaaacgtt gttaaatcaa cgttttttta ttttggtcaa ggagagaaaa gtagagaaat
     5881 acaaaatttt ttgcattgat tttgcattca aaaaaacatt caaacattta gaaatcccca
     5941 atacgtattc aggaaaatct attttaaggt ttacaaaaag aacttttgtt cgtacactct
     6001 ttttgaggtg aacttttatg ctgatggaag gaaaaacaca attatggttt aagcatgttc
     6061 cttcaaata
//
LOCUS       DAZAJZ010000132         5924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000132 DAZAJZ010000000
VERSION     DAZAJZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.136"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..621)
                     /locus_tag="ACG86D_002737"
     CDS             complement(136..621)
                     /locus_tag="ACG86D_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141809.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(760..975)
                     /locus_tag="ACG86D_002738"
     CDS             complement(760..975)
                     /locus_tag="ACG86D_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN3141810.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1199..1999
                     /locus_tag="ACG86D_002739"
     CDS             1199..1999
                     /locus_tag="ACG86D_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3141811.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2018..3886
                     /locus_tag="ACG86D_002740"
     CDS             2018..3886
                     /locus_tag="ACG86D_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN3141812.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3879..4370
                     /locus_tag="ACG86D_002741"
     CDS             3879..4370
                     /locus_tag="ACG86D_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN3141813.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4358..4912
                     /locus_tag="ACG86D_002742"
     CDS             4358..4912
                     /locus_tag="ACG86D_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN3141814.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4930..>5924
                     /locus_tag="ACG86D_002743"
     CDS             4930..>5924
                     /locus_tag="ACG86D_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN3141815.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2070 a          946 c         1195 g         1713 t
ORIGIN      
        1 atattcataa gagaaatagc tgtaacgaat tattaggtta atgcaaatgc ttatttatac
       61 tccgtataac tttctcagaa aaaatgatac caataatgac ttgctagtac tgtaaatccg
      121 tataatatca tctaatcaaa atggtgttcc atttgcatct gtcatcgtaa tcgatccttc
      181 gggtgacact cgataaaaac caacgcttcc agatcctcct tgggaacgga tttgtttgct
      241 ggaagctgtg aacaaataat ctcgatcaat tttttgataa aagctaataa caatatcgtc
      301 actttgtaat tcggaacggt gggcaataag aaatagaagt gctttgtcag agttttctaa
      361 tggaagagaa ggtacagtgc tttcttgcat ttcagatgtt gaagaaatgc taaacgactg
      421 aatacttgat tggctatgtt gcgaatctac attaccagta gaagtattag taatggaagt
      481 agtagaagta cttttagttg aaccaacctc agttgttgaa atggctgctg aagattcttt
      541 aacagaattc tttgttttat atccacaggc agaaaaaagt attacagttc ccaacaagcc
      601 tgcgtaaatc cctttatgca tgatagctcc tcctaaaata gattatcttt attcagtata
      661 tcacttaaac aaagaaaagc agatggttta aaatgaaaaa accttcccaa taaagggaag
      721 gttcatgaag ataataactt ttttaataat tatagctttc taatatcttt catcaacttt
      781 tctagtaatc attgggaaaa atttcaagca gtagatcgca caaataccta cgacgatata
      841 aatgatcttc gcaaaaatag ttgttgaacc accagcaatc atagctacta aatcaagttc
      901 gaacaagcct accaacagcc agttcaaccc tcccacaatc aataaagtta atgcaatagc
      961 atctaaagtt ttcattagaa ttctcctttc ttaaatgata aaacaattat atatcaaaaa
     1021 gagaaacatt gcttatataa cgctcaggaa ttttagcaat ctatgcaaaa agatgaaaat
     1081 tttttttgct cttccattag tacacatttt ttagctgaag ctgtaaattt catgtcttaa
     1141 ttttttttag tgagctttta tactggattt gtaagaaaaa agaaggagag gatcacatat
     1201 gtcggattgg ttggatattg caggaaaagt agtaattgta actggaggtt cctcaggaat
     1261 tggaagaagt atcgtcgaaa atttgttgaa gcagaatgca caagtagcga attttgatgt
     1321 aactgaatgc cgtatacagc atgaaaacct actatcttta aaagtcgatg tgtcttctaa
     1381 aacggacata gaagaaggga tatataaagt aatgaaacat ttcaagacga tcgatggtct
     1441 agtcaataat gcaggaataa atattccttc tcttctaata gatagaaatc atccaaaaag
     1501 caagtatgaa ttatcagaac aagtttttga taagatgatc gctgtcaatc aaaaaagtgt
     1561 atatttgatg agccaagcag ttggaagaat cttggtgcaa aagggatcag gagtgatcgt
     1621 gaatctttct tctgaatcag gattggaagg atcagaagga cagagctgtt atgctgctac
     1681 aaaagcagcg atgaacagtt ttactcgttc ttgggcaaaa gagttaggaa agaggaatgt
     1741 tcgtgtggta ggtgttgcac cagggatact ggaagaaacc ggtttgagaa cacaagaata
     1801 tgaagaagct ctttcttata cgagaggtat aagcgtcgaa cagctgagaa acggttacag
     1861 tcgaacaagt acgatccctc taggaaggag tggtaagctg caagaagtgg cggatctagt
     1921 ctgctattat ctttcagaaa gatcaagtta tatcactgga gtcacgacta atatttcagg
     1981 aggtaaaaca agaggataaa ggataggggg gacgcacgtg acgctagtaa acagatggta
     2041 ccaattattg aaattactag tcactcacaa gaaagtttca atccaggaag tgcaaaaaga
     2101 aatcaaaacc agtaatcaaa ccataaaaaa agatattggg gagttgaatg aagaaattgc
     2161 cggtattgca aaaatcatcc aaaaaaacaa acattttgaa cttgaaattt atgattttga
     2221 tttttttgat gagatcatgc aaggaaaatt gaaaacagcc agtgatttca attcggcatc
     2281 caaaagaatc gcttatttac taaataggct gatcgaaaca gaggatttct tacgtatgga
     2341 tgatttgtct gaagaagtcg gtgtcagcag aggaacagtg gtcaaggatc taaagacatt
     2401 aaaagagatg atcaatgatt ttgatgtaaa gattacaggc acaccaaata aaggactaag
     2461 aataatagga gatgagctag aattacgact acttttattt tattttgttt ctccctactt
     2521 ttcagagacg aaaacagaac agtttctaga agaaaaacaa gcaatcaaac attttcagaa
     2581 gaagacgaat gctagtaaac aagaaatcac ccttttaacg aaagttatcg gtatttcttt
     2641 gaatagaatc tctagtaact atctgttgaa gaagcccatc aatcgttaca gtgggtgttt
     2701 tgaatatatc caagaattca atgaactgat caatcagttg gaagaaatct atgaactaac
     2761 tttgagccaa tatgaaaaag attttatcag ttttcctttg aacatcatca acacaagtat
     2821 aacgatgata aggaaagcta actctgattt gcgcttatat ttcgacccca tgatgatgcg
     2881 tattcgacga ttagatctca ttgatctgga tgagtccttc ttatatcaag agatgaaaca
     2941 gcacctctct ttattgataa atcgggtaat ttttcgttat cgattgactg atttatttta
     3001 tggtgagata gaaaaaaagt atcctttttc ttaccaaatc gctagcgaat gcatacaagc
     3061 attagaagct atcttgcaac ataagatctc taaagtagag atcagctacc taacgttgta
     3121 tttggagtta gcatttcgat atcaaaaaga gcatttttcc tctaaaaaag tagccattgt
     3181 tcatacgaca ggaaaaggaa cagcattaat gatccaaaga caaatcaaaa ggatattggg
     3241 gagggatata caaataacac atgtttcaga agaacaatat gaaaaagtag atctaaacaa
     3301 atatttcgct gtttttacaa cgattccgtt aaaaaatgta aatgaagaga caccggtgat
     3361 ccatttgact aatctttttg atgactcttg gttaagggca caatggcaaa aagcaaaaaa
     3421 aaagcgatta gccgggtttc attctactgt ttttttctat caaatatgga aagaaaaaaa
     3481 gacgtatcaa gaatatttaa acaaattagt cgctcctttg atcagaggct attatgtgga
     3541 tgaagaattt gaaaaaagga tcttgaaaag agaatatcag caaagcacat ttgttaacaa
     3601 agaatttgct tttcctcaca caaccaacca gctatctcaa cacattgttt tatcagtagg
     3661 gctaataact gatcaaggtt tagcagaaac aggaggaaat ggagttattt ttttatttgc
     3721 catacctgaa gaaatgacgg ctgaagttga gaatgaatta tttgagctat gcgatatcct
     3781 atttgaactc ataaaaatgg gaatagatga gtttattgaa ttagcaccga cacaagaatc
     3841 atttttaaaa ctaatcaaaa gtaaaggaga gagacttcat gaatgatatg tatctattca
     3901 tagttttcgc agcttcagct tatgtgctgc aagcagttat cagttggaag caactaaagc
     3961 atttcaatca aacatatagc gaactgcgaa gaaaaggaaa agtagcgatt ggtaaacgac
     4021 ctgggaaaat ccgttcaggc acgattgtta tgctagctat agacgatcat gatcaagttt
     4081 tagaagtcag aaaaatgcaa ggggttacat ctttggcaaa atttcattca ttacctgtct
     4141 atgcgggtga agatatccat tacatcgatc gttaccatcc ctcagttcga gcggaagatc
     4201 gtttgacaca agcagcttta gaaaatgcca gagaagtata cttacgaata gaggcaggaa
     4261 atgaaaaaag tgaacaacct tcattgcctt ttattgctat gctcaataat tttcggatat
     4321 ggaaaagtac gatcaaagaa aaaaggagtg acaaacgatg gatatcataa caaaatttgc
     4381 tgagggattt atgaggctgt tccaaacagg tgcagaaaca ttcataagct ggatgacagg
     4441 aatcgtacca gttgtcttga tgctgatggt tgcaatgaat acgctgattg cttttttagg
     4501 tgaagaccga gtaacgaaag tagccaaatt atcagcgaaa aatccgctta ctcgttacct
     4561 tgtgttaccg ttcttatctg cttttatgtt gggaaatcct atgtcattta caatggctcg
     4621 tttccttcca gaatactaca aaccaagcta ttatgctgct caagctcaat tttgtcatac
     4681 gagcaatggt gtattccccc atattaatcc tggagaattg tttgtatgga tggggattgc
     4741 tcaagggatc gaaaccttag ggctaaattc gatggagctg gctatccgat acttgcttgt
     4801 gggactgctg atgaacttta ttggtggttg gataactgat tttactactg gatttgtatg
     4861 cagacagcaa ggaatcgttc ttagcaaaaa agtagagctt tcagttgatt agagaaagga
     4921 gagttttaaa tgtatggtga tattaagatc gtaagaggaa acggcgggta tggaggtcct
     4981 ttgattatta ggccgacaaa agataaacac aagttcatct atgtcacagg tggaggtgag
     5041 aggccaatga tcgtagataa aatagaagaa ctgacaggta tggagtctat caatgggttc
     5101 aaaacatcaa tacctgatag tgaaatcgcg cttgcaatcg ttgactgtgg agggactctt
     5161 agatgtggaa tttatcctaa aaaagggata ccaaccatca atatcgtttc cacaggaaaa
     5221 agtggcccat tagcacaata tatcactgaa gatatttatg tttcagcagt agggttagaa
     5281 caaatacagc cactaatgga aactgatgat aacaaagaaa cagtaaatac tgcatctcca
     5341 gataaaaagt acaaatatga tacttctaaa aagataaccg agcaaaaagc agagcagcaa
     5401 aactggatag caaaaattgg aatgggcgcg ggaaaagtag ttgctacatt caatcaatca
     5461 gcaagagaag cagtagatac gatgatccgg acagtgatcc cttttatggc tttcgtttcc
     5521 cttttgattg gtgtgataca aggttctggg atcggtaatg tatttgctaa cattatggca
     5581 cctttagcag gaaatgcagt tggattaaca atcattggct ttatttgttc actgccattt
     5641 ttatctcctt tgcttggtcc aggggcagtt attagccaag tgattggaac attgatcggt
     5701 gtagagattg ggaaaggaaa tattcctccc caattagcat taccggcact atttgctatc
     5761 aatactcaaa atgcatgtga ttttattcca gtcggtctag gtttggaaga agcaaaagct
     5821 gagacagtag aagtaggtgt accatcagtt ctttattcaa gatttcttaa cggcatccca
     5881 cgagttatca ttgcttgggt agcaagtttt ggattatata gctg
//
LOCUS       DAZAJZ010000133         5769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000133 DAZAJZ010000000
VERSION     DAZAJZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.138"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG86D_002744"
     CDS             complement(<1..300)
                     /locus_tag="ACG86D_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN3141816.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG86D_002745"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG86D_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG86D_002746"
     CDS             complement(1014..1304)
                     /locus_tag="ACG86D_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141817.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG86D_002747"
     CDS             1706..1942
                     /locus_tag="ACG86D_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141818.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG86D_002748"
     CDS             1949..3901
                     /locus_tag="ACG86D_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN3141819.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG86D_002749"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG86D_002749"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN3141820.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG86D_002750"
     CDS             complement(4797..5489)
                     /locus_tag="ACG86D_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3141821.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1893 a          997 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DAZAJZ010000134         5737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000134 DAZAJZ010000000
VERSION     DAZAJZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.139"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            312..1037
                     /locus_tag="ACG86D_002751"
     CDS             312..1037
                     /locus_tag="ACG86D_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFN3141822.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            1027..1701
                     /locus_tag="ACG86D_002752"
     CDS             1027..1701
                     /locus_tag="ACG86D_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFN3141823.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1989..2273)
                     /locus_tag="ACG86D_002753"
     CDS             complement(1989..2273)
                     /locus_tag="ACG86D_002753"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFN3141824.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2567..3874)
                     /locus_tag="ACG86D_002754"
     CDS             complement(2567..3874)
                     /locus_tag="ACG86D_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN3141825.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4363..5469
                     /locus_tag="ACG86D_002755"
     CDS             4363..5469
                     /locus_tag="ACG86D_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141826.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         1883 a          982 c         1093 g         1779 t
ORIGIN      
        1 tgtagataca aataactgta acattgaaaa tgaatgcgaa aacgatattt ttttttattg
       61 gaaaatatcg gtcaagcaat aaaccagttg cataataaaa tgttaacggc aacaaaatct
      121 gtggaaggaa tgaaccggat gatttgatgg aaaaaacaag atagacaaga agtatacata
      181 gtaaaacgtg gaacaagtag gccagaatga aaaatcagtt tttatccact cgcttttcta
      241 tacattcact gtcacaaata taatcgaaag taattctatt ttgagataaa gtattttata
      301 ggagatagta tatgaacaaa aaaagattta aacgtattga aagaaaattt gcattaactt
      361 atgaccaagc taagtatttg aaaacactat ttccaaaaca ggtagttcgg gataaatata
      421 gtgatcattt tatttcttcc ttgtattttg atacaaacga tcatcacttt ttaaaagaaa
      481 tcactacaga aaagtcctat cgagaaaaat ttagaatcag aaaatatgga gatcctcagg
      541 acatatcgcc tatctactta gagataaaaa gaaagtttca aggaatgaca gataaacgta
      601 gaataaaatt agcgcatcat gagctcgatg atatgttgga aaatatcgaa ttggcagaaa
      661 ccacattcag taatttagaa gaagagtata tttttaatga aatacagtgg ttgttcagaa
      721 ggttggcatt ggagcccaaa gtatatatct attatttaag agaagcattt gtaaatagag
      781 attcagaaga tttgcgaatc acttttgatc aaaatatcca ttaccgtcta atgaacttaa
      841 cgacctggga acactgtaca gaagacaatc tggtagcgcc agaaatcgat gtgctgatgg
      901 aagtaaaaac gacaggagaa atacctgaat ggttacaggt aggattggat aggatgggta
      961 aaaaagagat aaaattttct aaatataaac aagtctataa aagatatatc caagaggacc
     1021 cgctctatgt tcaatagtgt cttaaaaaat gagccttctc aactcacact agatgatttc
     1081 tttttatgta tggtggtgtc gattttgcta ggcattctga ttgcttacac ccatatgtat
     1141 caaaacaact gtacaaaaaa cttcatgttt gccttgattg ggttacctac tttagtacat
     1201 accgtcattt tgttgatcaa cggtaactta ggcacgggga tcgctgttct aggcgcattt
     1261 aacttagtgc gatttcggtc tgctcaaggg aattcaagag atattttcag tattttttgg
     1321 gcaacgtcga ttggtttagc cacagggacg ggatatattg tctatgcact attcgtgtct
     1381 agtatagttg ctgctatcca agtcatcatc ttcacgcttc caatcaaaaa taaacataca
     1441 gtttttaata ctgaaaaaac gatcaagcta aaagtttcta aatccttgtt tctttcgagt
     1501 attattgaag aaaaaattgc agaatataca gaaaactgca atttaaaaaa agtaaaaatc
     1561 aataaagaaa cttgttcctt acactatata gtaaaagtaa aaacgagtat gcttgaaaaa
     1621 gaattggttc gaacgatcat ggatcatcca gaagtcattt cgatcactgt aggtatggat
     1681 aaatcgaata aattattgtg ataaccaaga aagtacttaa caatgaagta agaccccagt
     1741 tccaaagttt agcggggaca cttcaataaa gtactttctt ttttgtcttc agcaactact
     1801 ggtaaaactg agagcaagct ttcgtgagag tacagcgata aagatcaacc tcccttttta
     1861 tctcgagcaa tttccttgca aggctgtttc gttgatctta aacgagttag caacagtcaa
     1921 agatgcgatg gccataaaaa aattgcatta agttggctgg tatctgatag ctgggaatct
     1981 aaggaagact atccattctg tttcttaaat tccgctagtt gatagatatc agccaattgt
     2041 tccagatgat caacatcaaa gataaccgaa agatgtttcc ttttattccc atttgcttga
     2101 agagttttta tcttctcttt ggttccttca ctcaatgaga gtgtgactaa tttctccctc
     2161 gttttttctg ggaaatgaaa ggatactttg aaaaatccgt cccaaataga aagccagaag
     2221 atcggtttta ctttctgaga gtgggacaaa agtaagaaac acttttgtct cattctttaa
     2281 aaacgaataa acggtgggag cagaagcaac tccttcggaa ataagccaaa attcacaaaa
     2341 atttgaaaag caattttcgt gaatttcttc ttatttctcg gagttaaaca cttctgtccc
     2401 aacctcttgt attcaccttt tgatagccaa gcttttccat cattataaaa ccgcaaattt
     2461 caaaaaaaaa cgaacaatac aaaaagcgta ttgctcgttt ttctcttcag tttctcacac
     2521 ctaaacgact tttgtctcga cctctttttg gtgactattc atgatgttaa aataaattac
     2581 tgaaaaaatc tgtgacatgg cgccaaccaa tgacaaaaat attagctttt tcaacagtag
     2641 aagtggtaat gatgtctgtc gaaggctctt ggtttgcttc caaatagcct aatttgtcat
     2701 cagctaattg gatcgttgct gagccaactg tagtgccttt gttgatagga gcggtcactt
     2761 ggtcatccgt tactttcttt ttatcaattg ttggagtaat cgttaatttt ccagtatcca
     2821 ttccattttc gacccagact tttacagggc tttttagcgc aacattgacg gaggtttctt
     2881 ttccatcttt cactttgatg tcttttaggc ttgggatgct tgcatttgct tttccaagtt
     2941 ctttttctga ccagttgtca taacaataat ccatcaatcg tgatgtttca ttgaatcggg
     3001 cacttggatt ttcttcatgg ttatctgcat ttaaaacgac tgtgatgatt cgtcggccat
     3061 ctttgttgat cgttccgaca aagcatgcac ccgcaaaatc agttgtgcca gtttttaatc
     3121 catcgacacc ttcgtgatag tttacaaaac caggtagcat ccagttccaa ttgaccattt
     3181 caaccggtga ctgtgtgttt tcgccgaatg tttgatttgt ggtactcgtg atttttaaga
     3241 cttcaggata atctaagatg aggtgacgag caacaatagc gacatctttc gcagacagtt
     3301 gattttcatc tgtttcacca gtattctcag gtcgattctc acctaggtaa gaattgttta
     3361 aacctgatgc attgacgatc gttgcatctt tgatacccca atcttttagt tgtttcttca
     3421 ttagctcaag aaatttactt tcactgcctg caactttttc agctaaagca accatggaag
     3481 cattggctga ttggatgaaa gctgagtcaa caagttcttt cactgtataa tcattttctt
     3541 tatgcaaagg aacattcgat agatccggca cgacacttaa tttttctgca taatccgaaa
     3601 tggctacctt atcttcccaa gataatttac cctctttgat ttgttcaagt acgatatata
     3661 gaccaatgat ctttgtgatg gaagcaattc ccatcgtttt ttcgccatcc tgatcataaa
     3721 ggatttttcc actttctgca tctacggcaa aggcagcttt ggcatttatt ttaaatgcgt
     3781 cgtctgcttg aacagttgcg ctaggcaaga aagaagtgat acataagata agacttgcta
     3841 atagtacaat aaaacgggta ttttttttga acatctttgt tctcctcatt tttaaattga
     3901 ttcttacaac ttagaatatt atcagagaga acaaaaggtt tcaatggttc tgaaaaaaac
     3961 cttataaaga acaagtctta ttaatgaaga atcatagaaa gttatctttt gggttcattg
     4021 acacgaactt tgactggctg aacttcgggt tcggttaggc gtgatgctgc aaaaagggcg
     4081 cctgctgaga gtgcgaccaa actaaaaatc atgaataatt gcataagatc acctcgttct
     4141 tttttgtttt tttatagtat agcaagagag tttgaagcta ctgttaagga aatgagcata
     4201 taattgatga aagtatgaaa aaaatatgaa gtaggacaac acaactaaaa atcgatcagg
     4261 tatttctctg gtagggattc gttttattgt attgataaaa tgggatgtgg tagactttat
     4321 ttacagatac aaagaaatga tttttttagg aggggtacgt ttatggcaaa aaaagaaaaa
     4381 gaattattat cacaattgac ttcagaaggt accaaaggac cgtttgtcac gattatgtta
     4441 aacacccatg ttgcgcatca agacgtagaa aaagatcaaa tcaaattcaa gaattttgca
     4501 aaggaagcga aaaagcgctt tgaaaagaaa tactctgagc atgattggtc tccattccaa
     4561 gaaaaaatta ctagtttgtt ggacgatccg tctttctggc gtagtgccac aacgagtgta
     4621 gctgtcatag tatcaccaga acaagtgttt atccaccgtt tgggtattcc tgtagatgac
     4681 cagtattatg tgagtgatct accttattta ttagcgatca tcaaaaatgg acatttcaac
     4741 tattcatact atttattagg attgaatcgt gattcgatga aattgtacga agtaacgaat
     4801 aaacaagtca aagaagtggc tttgcctgaa gatgcaccaa gagatgtcgt cactgcttta
     4861 ggatctgaac taacaggagg cgggttgaac tacgcatcaa atggtagtga aggtacgacg
     4921 ttccatggag tcaatcctaa agatgaagaa gtcgaaattg attgggtcaa ttattatcaa
     4981 gcagtcgatg actatttgaa agatacattt gaaaatccag agaaatggcc tatcttctta
     5041 tttgcattac cagaaaatca gacgatgttc aaaaaagtgg cgaagaaccc ttattatcgt
     5101 tccgatgttg cgatctcagc ttcacctgca caactttcat taaaagaaat tgaagcaggc
     5161 gcaaaaaaac tatcgcaaga attagctgtc aaagaagcaa gtgactacaa taagttgctt
     5221 gataaaaagt tcattgatca actcgttgat attataccgg cagcaaaaga agggaaaata
     5281 tcccatttat tcattgctac atctaattta gcagatggtt ttggcgaaga tccagaaaca
     5341 gaatatgacc gccgtcaggt gctgaatgaa ctagcagata atgtgatcac gaacggtggt
     5401 caggtcttta tccttgaaca aaaagatgca ccagacgaaa agagtttagt tgcgatctta
     5461 cgttattgag tgaaaactac caaagtagat gaatggtcag gcgttgatag aagtgtatag
     5521 ctccgagaga aggagtttcc tctgtttcag ccgttggatt catcttgagc gagagacaaa
     5581 agctagcttt tgtctctcgc tcattatttt tgtgctttag tcccattggc tttgcgaggg
     5641 gaagatactt ttctcaaaaa aacgaacctt gcagttgaga gggggataga tgcgcatact
     5701 aatttttatc agttaaaaaa tgatgtggat cattaga
//
LOCUS       DAZAJZ010000135         5503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000135 DAZAJZ010000000
VERSION     DAZAJZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.140"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACG86D_002756"
     CDS             complement(56..1561)
                     /locus_tag="ACG86D_002756"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN3141827.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACG86D_002757"
     CDS             complement(1653..2243)
                     /locus_tag="ACG86D_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3141828.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACG86D_002758"
     CDS             2422..4068
                     /locus_tag="ACG86D_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3141829.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACG86D_002759"
     CDS             4117..4836
                     /locus_tag="ACG86D_002759"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN3141830.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..>5503
                     /locus_tag="ACG86D_002760"
     CDS             5315..>5503
                     /locus_tag="ACG86D_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN3141831.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRES"
BASE COUNT         1660 a         1003 c         1113 g         1727 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agt
//
LOCUS       DAZAJZ010000136         5341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000136 DAZAJZ010000000
VERSION     DAZAJZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.141"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG86D_002761"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG86D_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG86D_002762"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG86D_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACG86D_002763"
     CDS             2090..3130
                     /locus_tag="ACG86D_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN3141832.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /gene="traE"
                     /locus_tag="ACG86D_002764"
     CDS             3954..4286
                     /gene="traE"
                     /locus_tag="ACG86D_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN3141833.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="ACG86D_002765"
     CDS             4905..5246
                     /locus_tag="ACG86D_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN3141834.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1771 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata
     4861 taaaacatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
     5341 t
//
LOCUS       DAZAJZ010000137         5317 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000137 DAZAJZ010000000
VERSION     DAZAJZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.142"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..822
                     /locus_tag="ACG86D_002766"
     CDS             31..822
                     /locus_tag="ACG86D_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3141835.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            788..1741
                     /locus_tag="ACG86D_002767"
     CDS             788..1741
                     /locus_tag="ACG86D_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFN3141836.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2139..3113)
                     /locus_tag="ACG86D_002768"
     CDS             complement(2139..3113)
                     /locus_tag="ACG86D_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3141837.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3228..3662
                     /locus_tag="ACG86D_002769"
     CDS             3228..3662
                     /locus_tag="ACG86D_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3141838.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3787..4809)
                     /locus_tag="ACG86D_002770"
     CDS             complement(3787..4809)
                     /locus_tag="ACG86D_002770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFN3141839.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5201..>5317
                     /locus_tag="ACG86D_002771"
     CDS             5201..>5317
                     /locus_tag="ACG86D_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFN3141840.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIE"
BASE COUNT         1676 a         1013 c          948 g         1680 t
ORIGIN      
        1 attgactttt atgttaaagg agtaattcat atgctgaaaa aagaaagaca agaacaaatc
       61 attacattat tagagaaaga tttttattta actaattcag aaatcgctaa caaattaggc
      121 gtatcagaaa tgaccatacg ccgagatgtc actgaattag caaaaaaaaa taaagtaaaa
      181 aagctatatg gtggcgttga acgaaaggaa ccttataatc gagaactatc gactcaagaa
      241 aagattgata cgaatgtagc cgaaaaaaaa tatattggtc aagtcatgaa cagtatgatt
      301 cccgataatt ctactattta tgtcggtgct ggaacaacga tcctatatgc acttcctttc
      361 attcaaaaga aaaatctatt tgtaattaca aacagtttga ttgctttcaa ctatttaaag
      421 aatcatacaa gttatcggat tttacttact ggtggcgaat tttctcctat tacagaagag
      481 ttcattgggg aagttgcaga aagatctttt gaaagtttga atgtcgatat ctctttcgct
      541 gcaacaaacg gcatatttga taacaatgtg acaaccgctc aatttattga gggaaatatt
      601 cagaacgcag cgctcaatgc agcaaaaatc aaatgcattg ttgcagatta ttcaaaatta
      661 aataaatcag acgtctatac ttttagaaaa ttaaccgatt ttgattatct aataaccgat
      721 aatcatttat cagaacagga tttcaatcat tattcaaaat acttaacaat attaaaggag
      781 cccaaaaatg atactgacaa tcacgatgaa cccatccatt gattccattt attttacaaa
      841 acactttgaa ttaggtacga tgacacgctt tgattctccg gtacgctatg taggcggcaa
      901 gggaatcaat tgcggacgag cttctgctct actaggttca tcagttattt taacagggtt
      961 tttgggcggt tattttggca gtatgattca agatatgctg aaaaatgaaa atttgtttga
     1021 tttgcatttt ttacctattg cagaagaatc aagaggtgcc gtcacgatca tgcatgatga
     1081 tgaaacgcag acagagctag tggaagctgg tcctgaaata gcagatacta ctatcaaaaa
     1141 attaatgaat gatatcaaac taatcagtaa aaataattac ctttcagtca ttacaataaa
     1201 tggttctgtt aacaatccag atcccttttt gtatgctaaa ttattacaat atatcaaaga
     1261 acaaatagat ccttccacct atatattaat ggatatatct ggacaacatc tccgtgaagt
     1321 cttagcaagt aagtataaac cgatgttcat caaacctaat atccatgagt tttcagatat
     1381 catccatcaa aaaattactc aaaaaagcga cataaaacac catctaaaaa caaatccact
     1441 atttaaagat atcgatgtta tcatgatttc ttgcggctcg tatggtgcag tagtcaaagt
     1501 aaaagaagaa ttttggaatg ttgagatccc taagattcct atcattaata caacaggctc
     1561 tggggatgcc actgttggag gttttgctca ttcattagaa cagaattatc ctttaaaaga
     1621 atgtgtaagg tacgcaatgg cttgcggcat gtcgaatgca cagcaatcag aagtcgggaa
     1681 agttattcca gctgatgtaa aaaatttagc aaaaagcatt aaaatcacag aaatgaaata
     1741 aatagtacaa agtaaataat tgtttatatg aaatgagaca caaagagact gtgacaaaaa
     1801 acgtgtgtac cgacccccaa aagttagatt ttttggtcta acttttggga gtcgatacat
     1861 tgtcacagtc cttttttcga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     1921 caatattcaa caaacatgag aagctatttt ttgaaattgc tccttgtgac tcagagttaa
     1981 acggcaggct cacgacctct tggtataaac ggtgttcaaa agcttgcatc tacgctaatg
     2041 agtccaacca agtcaaaaat gaggagcttt tttggctgtc cttattactt gatgcagggc
     2101 agttttttca taatctcttt tagattaaat ccttttggtt acacaaggtt ctcttcaaag
     2161 aatgttgcca ttttttcaaa tggaatgatg tctgtctggt cgtatagatc tgtatgggat
     2221 gctcctggaa tgatcatgag ttccttgttg tcacctttta gttctttgaa agcgtcctcc
     2281 ccaaagtaac gtgaatgagc tttttccccg tgaatgacta agactgcatg acggatttct
     2341 ggaatataat gcaatagttt tgcattcata agtgacaacc ctgatgtcct attccaacca
     2401 tcattggagt ttaaagaacg gacatgatat ccgcggtctg ttttataata cgcatgatag
     2461 tctttgacaa attgaggagc atcttcaggc aacggatcaa ccacacctcc agcacgtgcg
     2521 tagtcccctt gtttgaaatc aatgatgcgt tgtgcattta attcttcacg catttcatat
     2581 cgggcatttt catccataac atcgaagtag ccatttgctg ttactcgagt catgtcatac
     2641 attgtggaaa caactgttgc tttgacccgc gtatcgatag ctgctgcatt caatgccata
     2701 cctccccatc cacaaatacc aatgattccg attctctcag ggtttacatt tttttgtaca
     2761 gataaaaagt ctactgccgc ttggaaatct tccgtattga tatcaggcga agcaacatat
     2821 cgaggtactc ctccgctttc tcctgtaaac gatggatcaa acgcaatcgt taaaaagcct
     2881 ctttcagcca tcgtctgcgc atatagacca gacgattgtt ccttcactgc accaaatggc
     2941 ccgctcacag cgattgccgg caatttcttt tcacttactt cttttggttc atataaatca
     3001 gcgactaatg tgataccaaa acgattcggg aagctcacct ttcgatgatt gacttgattg
     3061 ctttttggaa atactttgtc ccattcttgt gtgatgttta attctttatt catattctag
     3121 actctccttt gatcaaaatt gacatttgtt tttgaatgta ttattatgat aaaacttaaa
     3181 cttgacttta ggtcaaggta aaaattttat tttaggagga tttttttatg tattccattg
     3241 gacaagtgtc tgaaatgttt ggattgcctg tttctacttt acgttattac gacaaagagg
     3301 gattatttcc tgatatccag cgtgtttctg gtattagaaa attttcagag aatgagttag
     3361 aggctttaag agtcattgac tgtttgaaaa aatctggttt agaaataaaa gacattaagc
     3421 aattcatgga atggagcaaa gaaggcagta agacatttga ggttcgtaaa gaactatttg
     3481 aaaaacaaaa agaagtagta gaaaaagaaa tagcaaaatt agaaagagta ctcgctatgc
     3541 taaactacaa aagctggtac tatgaagaag caataaaagc cggcaatgaa gaagccgtat
     3601 tgacaatgat tcctgatgat ttaccccaac atgtaaaaga agcttatgta cattctcatt
     3661 gaacaaacgg ataactcact ctccttctct atgaacttca tgaaaacatg aataaacacc
     3721 tctaactgat tgttagaagt gtttattttt tcactcaagt atcctgcact tacaaaaata
     3781 tcctttttac cgccatccta attctggagc gatatctttt acgattgttt caagcaagtg
     3841 catgttatat tctaccccca aagtattagg aatcgtgaca agtactgtgt ctgcttcttt
     3901 gattgcttca tcttcagcta gctccttgat taattgatca ggttcaccag caaaagtttt
     3961 tccaaaaatc gttgggtttc ggtgataagc aagatagccg acagcatctt gttgcggact
     4021 accgtcttgt ccaaataagc gtcgatccaa atcagtggtg attggctgaa tcgagcgagt
     4081 aactaatgtt cttggttcaa aatcatgacc tgcttgcttc cacgcttctt tatacgcacg
     4141 taattggttt gcttgttgga tatggaatgg ctcgtgggat tcgtaattga ctaatgtaga
     4201 tgattgcaga ttcattccat gttgtgctgc ccagattgcc gtttgctgcg aaccagctcc
     4261 ccaccagatt ctttgtcgta atccttctga ataaggttct atccgtaatt ttcctgatcc
     4321 ttgtgggaac attggttgag gatttggttc ttcaaatggt tttccttcaa gtaattccaa
     4381 gaattcaagg gtgcgttccc gtgttacctc tttgatttct tcttcactgt aatcataacc
     4441 aaagtatttc cagccatcta gaacttgttc aggtgaacca cgccccactc ctagttgcac
     4501 tcttccttgc gtgatgatat ctgtcaatcc ggcattttct gccatataat aagggttttc
     4561 atatcgcata tcaataagtc ctgtaccgat ttcaatctta cttgtctttg cgccgattgc
     4621 cgctagcaaa ggaaacggcg acccaatctg aggggcaaaa tgatgtacgc ggaaaaacgc
     4681 tccgtccact ccgatttttt ctgcttccac cgctaaatca atcgattgca aataagcatc
     4741 ttgagctgat ttcacaagcg atccttgctc catccaatgt ccaaagctta aaaagccgat
     4801 ttttttcaag gtaatgacct cctttaaacc tacaaaaatg gaaggttttt tatttttaat
     4861 atgaactgat tctactactt tttttcttaa aagacgaata attgttctcg cctcacaatc
     4921 aagaaatgta tcggcttact acacccctac tttcttcatc atctgccgtg tcttaatctc
     4981 ttttaacagt ctgttacatc gttgattttt gactcaaaat gattgtttct aacatgtatt
     5041 tatggtaaaa aagacaagat tagttctttg tacacatcga tttatacgct gaacaaaaag
     5101 aaatatataa actaagaaac ccattgtttt ttcgctccat cacatagagt ttactttaga
     5161 taaacagtga catttactaa tccataacgg aggaaatcaa atgaaaaaaa tctatttctt
     5221 atgtacgggc aattcatgcc gcagtcaaat agcagaaggc tacgcgcgta aatacttgtc
     5281 tccttcaatg tttgagataa gaagtgcggg gatcgag
//
LOCUS       DAZAJZ010000138         5312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000138 DAZAJZ010000000
VERSION     DAZAJZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.143"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG86D_002772"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG86D_002772"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN3141841.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG86D_002773"
     CDS             complement(1297..2133)
                     /locus_tag="ACG86D_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141842.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG86D_002774"
     CDS             complement(2317..3534)
                     /locus_tag="ACG86D_002774"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3141843.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG86D_002775"
     CDS             complement(3683..3844)
                     /locus_tag="ACG86D_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141844.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG86D_002776"
     tRNA            3917..4000
                     /locus_tag="ACG86D_002776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACG86D_002777"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACG86D_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAZAJZ010000139         5192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000139 DAZAJZ010000000
VERSION     DAZAJZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.144"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(336..1205)
                     /gene="scrK"
                     /locus_tag="ACG86D_002778"
     CDS             complement(336..1205)
                     /gene="scrK"
                     /locus_tag="ACG86D_002778"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN3141845.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1225..2346)
                     /locus_tag="ACG86D_002779"
     CDS             complement(1225..2346)
                     /locus_tag="ACG86D_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN3141846.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2348..2743)
                     /locus_tag="ACG86D_002780"
     CDS             complement(2348..2743)
                     /locus_tag="ACG86D_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3141847.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2762..3559)
                     /locus_tag="ACG86D_002781"
     CDS             complement(2762..3559)
                     /locus_tag="ACG86D_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3141848.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3561..4349)
                     /locus_tag="ACG86D_002782"
     CDS             complement(3561..4349)
                     /locus_tag="ACG86D_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3141849.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4363..4839)
                     /locus_tag="ACG86D_002783"
     CDS             complement(4363..4839)
                     /locus_tag="ACG86D_002783"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3141850.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4911..>5192)
                     /locus_tag="ACG86D_002784"
     CDS             complement(4911..>5192)
                     /locus_tag="ACG86D_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3141851.1"
                     /translation="ERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLE
                     DEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1629 a         1129 c          767 g         1667 t
ORIGIN      
        1 tataagagag ggaggtaatg gtctgaacac caagaattgt tccaacatcg aattgcttaa
       61 ctaatgagtt caaagcttaa ttatgtggct cttatattta ccgagcgtgg attgcgatct
      121 ctttagcgaa acaaacatct ttcataaaaa aaacagccat atccgctttc ttggatatga
      181 ctatttacta ctactttact atctttcatt cttcttgtct cacttatgat aaggcgctgg
      241 gtatcatgag tgaatgatat tgctgaatac aaatagtccg accacattaa gttgatcgga
      301 cttcataagc attttattga ctaatacttt tttgcttaag aattgattct aacatctttt
      361 gctaaagtta agcatcctat agttcctgca ttatctgcta acttaggtgt gacaatgtat
      421 tcttcgatat ttggcgtatc gacatagccg ttaattagtt ctaaaaaacg tctttgtact
      481 ttctttttca gatgttcttg ttgcatgaca ccaccaccaa aaattataat atctggtgaa
      541 agcatcaggg tcgtattaca ggcacattgt gcaatataat ccgcctcgat ttcccagaac
      601 ggatcttcta tagaaagatc ttgtcccttg gttcccgtac gcatctcgat agcgggtcca
      661 gatgccaacc cttcgagaca atcatgatga aaaggacaag taccagagaa agtatctcct
      721 gaatgtctct taactaacgt atgccccatt tctggatggc taaacccttc aacaaaaatc
      781 ccatcttgta tagctcctcc accaattcca gttccaacag tgtaataaac aacactggat
      841 aaaccttttc catgacctga aacatactcc ccgtaacaag cagcattcac atcggttgtc
      901 caggcaattg gaatttcgaa atgcttattc aacgttccaa caaaatcaaa atttttccag
      961 gcagtttttg gagtagaggt gatatgtcca taggtctttg attcacgatg gatatcaatc
     1021 ggtccaaatg accctactcc aatgctttct aattgttctt tatagtgatt aaagaaatca
     1081 attaccaaac tcattgtttc attcggtgta gttgtaggaa aactcactct ttctaaaacc
     1141 tttaaatcat cattgccaat ggcacaaaca aatttagtcc cacctgcttc tattgagcca
     1201 tacatttctt actccaccgt ccttttatct aggaattcgt tcttccctgt ctaatagttt
     1261 ttcgaaaaac tcatgtggtt ccttttggta ccaataggca acagaagaaa tatcgtcttg
     1321 acgctcataa agcttgatgt catcatttcc tagggtttgg aaagtaacac gtaaattttc
     1381 tttgaaacta atggggtcgg gtaaatgcca gcggtacaac ccatgcatcg gtaaagcatc
     1441 atcgccaaaa ccatgaactg gatttggatt aggttttcca gcactaaaat aatcccgggt
     1501 gtgatctctt ttagattgaa aaggatagcc cataaatagc gtattaaaat tttgagcata
     1561 tgccctttct ccaaagttct ttttatggaa agcccaagcg ccgccaaaat aatcttctgc
     1621 tcctgtagat gttattgtag ggaaatcttc atctccatct atataaaact taaattctcc
     1681 ttccccccac cagtatcttt ccaatgcgca caaagcaaag taggttccta cataataccc
     1741 agtgcctttg attccatcaa taagggtgta atcttttcct tttgtcgtga tgggttcacg
     1801 gttccattta gcatgaaaat acatcgcatc atccggtact tcagtcaatg cataattaaa
     1861 tgtataaaaa atgtgcttaa taaactcagg atgttcattc gttaaagtaa tcttagcttt
     1921 ttttcgaaat ggcatctcaa aatagcaatt catccctgat gttggattca ctacgattgg
     1981 taaggaattc acttcgcatc ttgtcccaaa cccattacaa aaaaaatctc ctaatggact
     2041 ttctacagaa ggagtggatt catcatccca ataaatacgc aaaattaaat cacgtaagac
     2101 gaaactccca gcgattgttt tgtctgtcac tgtcatccaa aaatgacgta aaattccagg
     2161 gccttccatt tctgctaaag taattgtctc tccagcattt atcggaacag aaggtgaccc
     2221 ttttctccca attcctagat gactcgctgt cattgccgct ttcccttttt caccattttt
     2281 attttctggt gaaatagttc tactttcctc tcccttgtgg agccatatat tatttaaaca
     2341 attcatttta ttcgtcctct atcctttctt cacttttttg attattcata tagatgattt
     2401 gttgttgtgc ctccgcaata attgctccaa tatctaaatt atcatttcct aactgcaaag
     2461 cgattgacat tgccaaggga agattcattc ctgcaattac gtgcactctg tcagaaatat
     2521 aggtaaaaaa cttttgattt acacttcctc cagccatgtc agttaaaaca attatttcgt
     2581 ctgtagtatc cactaactct ttaatttgtt catctatatc atcaatttct tcgacatatg
     2641 cacataagct actaatttga ttatgcatac ctgttaaaaa ctctagtgtc tctttaaaac
     2701 cctcagctag tattgaatgg ctagcaatga tcaatcttct cataattcaa atccttttcc
     2761 tttacgctaa tattccaaat gcagccataa ccatcgcaaa aacaataacg agtagaatca
     2821 aaatggtcat actgattttt ttcttactga gtagataata tgctgcagca gtaataatta
     2881 ctggaattaa tgttggtaaa attttgtcca acattggttg gattttcata ctaacttcac
     2941 caaaagtgaa tttaagtggc gttgaaacac tgataactga agcaattaat gatccaacta
     3001 ctgttagccc caatactgaa gcagcttcag ttaaataatt aatcttttca ccgtattcag
     3061 taacaatctt cattccctgc ttataaccaa attcaaagcc tctcattcgg aaaaagaaga
     3121 ttaccatgat taaaatgaac cataaaaaga ttcctacagg attattttct aaagccatat
     3181 aagccgcaat tgatcccatg atggttggga tcatcgtcat caaaagagag tccccaatac
     3241 cagcaattgg acccatcgta ccaactttaa agtcattaat cgcatctttt gcttctaatc
     3301 cttgcttttc ctccattgca acacaagcac ctaaaataat ggctgccagc caaggctgtg
     3361 tattgtaata ctgaaagtga ttatttaaag cttcgatgta ttcatcatca tctttgtata
     3421 attttcttaa aataggtgac aatgcgtaag tcacagatgc accttgctgt gtttgataat
     3481 taaaggtaca aactgacatc aaccaacgat agttcgcttt cctaagatcc tttttcgtta
     3541 cttgatattt ttcgctcatt ttattcatcc tccccaataa acccaacatc agaagctccc
     3601 gcagaagcag tgatttgtgc atctttttca gctctatgat acacattaaa tgctaaagcg
     3661 aatcctatga tcgccgctcc aataataggt accttcaaaa aagcggctaa gaaaaatcca
     3721 acaagtaaat aactaatgta tcgacctgta ggcataaagc gtaacaacat cgcaatacca
     3781 acagcaggca acatttttcc ggctaatgtt agcccatcca taaaccaatt aggtaaaaca
     3841 tccaaaacgt aattaacagc agactgtcct aaagtaacgg caataaatac tggaattgct
     3901 gcagataaac caaagaaaat tggccccaac cacaaaacac tattcatttt cttaaattct
     3961 cgtttattcg catattcctg tgatttgcga acaacaaaac catttaagat tttgacaatt
     4021 acatctagtt gcacccctaa catacctaca ggtagtccta ttgctaatcc ggcttctact
     4081 ccaagctggc taccaaatgc cgttgcaata attgcgcacg ttccatagtc cggtacagat
     4141 gagccaccaa aaccggcaac tcctaatgac atcaattgaa ctgtaccgcc aactatcaaa
     4201 cctgttttta tatcacccat caaaattcct gtgaataatc cccaaaaaac tgcgtaattt
     4261 acaaagatca tcggaatccc ataataatca acatgtttaa taaatgctaa tatagtcagc
     4321 ataataattt gaaaaatagt aaaatgcatt atccttactc ctttatccta attttgattg
     4381 aatttcctgt gccttatccg ccggaacaaa ttgtgtaaca atttctacac cagaatccaa
     4441 caatttttta tacgtgctga tattttcatc tgatgcatag gctctagaag atagctgtaa
     4501 tgcattatct ttaacaacag taccgccaac aattagtcgg tcaattctaa taccgttatc
     4561 tactaaatga aggattgttt ccggctcttt cacgatgaca aatacttttt cctgatcgta
     4621 tttccccttt ttaaaattcg caatcgctgt ttcttcagtg ataattgata cagccattcc
     4681 agcaggtcta gctaaacgca tacttgattt ttggatgtcg ttatttgcga cagaatcatc
     4741 gatcaccatc actctgtttg ctttagacat tggtgcccat tgcgtgacaa taatcccatg
     4801 caataaacgg ttatcaactc tcgacataac aatactcata aatacttcct cctttatctt
     4861 tcattttctt acaagcaatt accatgccaa aacaaaaaga acttgataaa tcaagctctt
     4921 tttacataat cataaacgta tttaacctca tgaatcggga tgtcgacact ataataatgt
     4981 tcaacgacac tgaaagattg cttaaaatgt gtctgaaatt tttgaaattc ctcactcgtt
     5041 tcattgaaat aaatttcatc ttccaaagta ttttgtttta ctaatcgttc aattaagcaa
     5101 ctaatgtgaa catataatcc gaaacacgta ttattatcaa atgaaattcc tagatcattt
     5161 tgtaagatgt atatagcatc tgaaacccgt tc
//
LOCUS       DAZAJZ010000140         5085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000140 DAZAJZ010000000
VERSION     DAZAJZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.145"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1859)
                     /gene="pepF"
                     /locus_tag="ACG86D_002785"
     CDS             complement(51..1859)
                     /gene="pepF"
                     /locus_tag="ACG86D_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN3141852.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2035..3033)
                     /locus_tag="ACG86D_002786"
     CDS             complement(2035..3033)
                     /locus_tag="ACG86D_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN3141853.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3259..3912)
                     /locus_tag="ACG86D_002787"
     CDS             complement(3259..3912)
                     /locus_tag="ACG86D_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN3141854.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4244..4642)
                     /gene="spxA"
                     /locus_tag="ACG86D_002788"
     CDS             complement(4244..4642)
                     /gene="spxA"
                     /locus_tag="ACG86D_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN3141855.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1512 a         1038 c          769 g         1766 t
ORIGIN      
        1 gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc ttataaacga
       61 tcgatcaatt cttctaattc atttagacgt tgttcaaaca tcgacattgc atcttcaata
      121 tatgcagcct gtgtcatatc aactcctgct tttttcatta cttccacagg ataatcgctg
      181 ttacctgctt ttaggtaagc caagtagttc tctagtgcct ctggttcctg gtttagtatt
      241 ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt attggaaaac atagtaatta
      301 taataaaaat gcggtatccg tgaccattcg aatttaattt ctgggtcttc ttcgactgct
      361 ggaccataat attttgcatt caatttacca taactatcac ttaggtattc actagtcaat
      421 ggcacacctt tttcatcttc cgtatgcatg aaatgctcga attcagcgaa ttgtgtctgg
      481 cggaaaactg ttcctttaaa cccatccaag taatggttga gtacataagc ccgtacacga
      541 gggtcttttt ctgtttccaa taaatactcc gttaggatat tttcattcgt tgtcgaagca
      601 atttctgcta aaaagatgga gtagtcgccg tacacataag gctggttcga acgagtgaaa
      661 taactatgaa cactatgtcc catttcgtgg acaagcgtaa atagctgatc cagcgtatca
      721 tgccaattca ataaaatata tggatttgtg tcatagcttc ccgaagaata agcaccgctt
      781 cgtttccctt tattttcgac aacatcgatc caacgttcag agaatgcttt ttctacgatg
      841 gccatgtatt cttcacccat tggtttcagt gcttctaaag ctttttcttt tgcttcttcg
      901 tacgtaaagg taattggagc ttcccctaag accggtgtat aaagatcata catgtgcagt
      961 ttttccactt ctaataaacg tttccgtaat tccatgtatc gatgtaacaa aggcaaatgc
     1021 ttgtttacca cgtctaccaa agtatcgtat acactttcag gaatatgatt attgctcaaa
     1081 gacgcttctc tggcagagct gtaattacgg actttcgctt taaaattatg tccttttata
     1141 tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt cgtaaacact gtacaacccc
     1201 ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc atacacgcca
     1261 tgagataatt ggactatttc accattttct ccttcaatcg ttggaaaaac tagatctgca
     1321 ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc tccagcaaga
     1381 agagattcct gctcagcaga taagacatgg gctcgattat ctactatttg ctggatataa
     1441 tggcgataga cttccaattt tggttcttct ttgaaatatt gccaaatctg gtcatctgac
     1501 aattgcaata tttctggttc aaaccaggaa acggcttcac taactttcga aaacagactg
     1561 cttgctcttg cataaagcgc ttggtaatct gtatttccag tgtcttgatc gtttttaagg
     1621 tgcgcatata cataaatgac ttcagtttgg cgataaaccc tcaatacgaa ttcaatcgca
     1681 tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga ttgttttaac
     1741 tcttctgata attccaaata tttctcatca aactcttggt cgcttgaaaa gatcttggtc
     1801 aagtcccaag ttaaattttc aggcaattct tctcgttttg gcaactgctt tacttccatg
     1861 aaatccactc cttttcttcc cttagctcta tctatcaaaa tagatcatag tctaacatcc
     1921 acatgtcaca gcatctaact tttctactca ttcatgtgtc tgcttccatc aattttagca
     1981 taactaaatc ttgttttaca tagaaaaccg attcctagcc tatttttata cttactattt
     2041 tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc tgatagacca tctcttcatc
     2101 aatcatagga aaaagccatt cccttttatg ttcttttatt tggcaaagcc atgagttgaa
     2161 atcaggaact ttctggtttt ccaaggcttc ttcgtacaac attcgataaa gaaaaacctg
     2221 ttcttgtaaa taaaagaaaa accgactttt ttgatagatc cacgatgata gatgcaaaag
     2281 atgtttatga cgcaagtagc attgttcttg tatggatact gtattctttt gcttgtgaat
     2341 aagttggcgt gtcagccatt tcttcccatc ttcactcact ttttctattt ttttcatttg
     2401 gttcatttta atttcgttac tagtgaatat ttctttaagg tccctactag aaaaagtcca
     2461 gcagctactg caaaaggaaa tatgccctga tacagtctct gtaatatgat gatacaaata
     2521 aagcttttgt ttcttgagat cagctttcca gcaatgcact cctctttttc tattatagta
     2581 gcagcaactt ttttctatca atgagaattg cgattgtcca agaaaagaat taccaagtat
     2641 ccaccatact tggtaactgt tattcaaata attttgggtt ctttcaataa atctctggtg
     2701 agacagtcta gaacattgga tttctattgc tagattcgcc aacaaaatat ccggcctttg
     2761 ccgcaacctc ggcaaatagg cttccaaaaa tactggttct actgactgtc ccagccaatc
     2821 catgaagcat tcctttaact gtaagtgctc ttttgattct ctttcactaa agaccgaaca
     2881 gttatctgat ttttgatggg caaaatgagc acattttttc tttccctttt tcaaaatcac
     2941 tctttttcga cagccacgac aataatattt ctttcctttt tctgcatgtt tagcaaaaca
     3001 gcacttatta tattcattca ttgcaagcag catttttctc cctctgaact tatttacgtc
     3061 taagaattta tttacacgaa aaaagagggc gtgaaaaagc tattctgcat caagtaataa
     3121 gaacagctaa acaaaaatag ttcccaatat ttttcgtttg gttgggcttg ttaccgcaga
     3181 tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg acacaatttt tgtcacagtc
     3241 tcttactata atgctgattt atacaaaata aaatttcgtt cgctcgatag cattacgcgc
     3301 catcagacat tgtccatatt cattcaatgt ttctggtgta cgatcgctct tttcagcaaa
     3361 ctccaaaatt cgagcaatct gattctctac atccgcttgg ttgagatttt ccatccagaa
     3421 gtgaacgact aggtaatagc gctcttcgaa taaatacaaa tcagtccaag cagactctag
     3481 ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa aagttgcttg
     3541 tctttcctgt tcttctaggg cgcttccaga taattcttcc tccggatcgc tgccaataat
     3601 ctgctgacga agaaagtcac tcacatcttc tgaatcaaca gtagaatctt ctgattgaga
     3661 aatttcttca tccggaagat ttttactgat aaacagttct aatccgtctc cttttggcaa
     3721 aacttggaaa gtgacagctt cgcttcctcg gaactcctct tcgatatcaa cttcttccaa
     3781 aatactataa aagaaatttt cgatttcttt gtgattgcca agtaaatcca aaaatgaaac
     3841 gccccgttcc tctaaatctt catgaccaat tagtacacgg atcgtatttt cattaatatg
     3901 ttccatttcc attttgctac acctcacttt gccacaatgt ttaatataga ttcattgtag
     3961 cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt aataaaacca
     4021 gcggattttt caacctttca agctatctta tttaactata acatttttgg cagacgaata
     4081 ctatatagcc gacttttcag ctaattttcc ctctctattt ttcgtgcaat catcatcttc
     4141 tactaccttt ttatacatct aatcaagaat actttttgta aaaacaaaaa aacttggaaa
     4201 aactatcttt gtaatagtct tttccaagtt tcgtattatc cttctataat ccagccatca
     4261 attgtgcttg acgtaattct aattgtcgta catcgcgcgg taggaaccga cgaatttcat
     4321 cttcattaaa accaacttgc agacgtttat catcaatcat gattggacgg cgaagcaatc
     4381 caggattgtt ttgcacaagt tccaataatt cttgtaacgg cagatcatct aaatccatgt
     4441 tcagtttttg gaatactttg gaacgtgtcg agatgatttc ttctgttccg tcttctgtca
     4501 tttgtaaaat cgcttttaat tcagaactgt tcaatggttc tgaaaaaata ttacgttcaa
     4561 caaatggaat ctgatgttct tgcaaccacg cacgagcctt tctgcaggaa gtacaacttg
     4621 gggaagtata taatgttaac aacgtgcacc actccttttc gtgataagaa tcactttttt
     4681 gtaacagaag atcataataa tctcccatct gtttctatta tacataatca acttttaaat
     4741 agctaccttt ttttcataat ttttttaaaa aaatgaaagt tatctaaggt taatatacag
     4801 taaaaccagc attctcgctt tttttatcat ttaaaaatta gagttttcaa atctaattca
     4861 aattttctaa acatatgaaa cagcgtataa aactgtacta ttattttatc tcaaaagcta
     4921 aatgaaaccg atttcttaag attttttatg ttttttattt ctttaatagt aatgttttct
     4981 gtcattttaa ataatactgt tctttattat aacgcttatt ttctctgtta tataagcgta
     5041 gtattttaaa gcaatgatta ctaaataaaa tttactataa acaaa
//
LOCUS       DAZAJZ010000141         4961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000141 DAZAJZ010000000
VERSION     DAZAJZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.146"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACG86D_002789"
     CDS             complement(139..1338)
                     /locus_tag="ACG86D_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3141856.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACG86D_002790"
     CDS             complement(1468..2844)
                     /locus_tag="ACG86D_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3141857.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACG86D_002791"
     CDS             complement(2844..3500)
                     /locus_tag="ACG86D_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3141858.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACG86D_002792"
     CDS             complement(3510..4787)
                     /locus_tag="ACG86D_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3141859.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..>4961)
                     /locus_tag="ACG86D_002793"
     CDS             complement(4806..>4961)
                     /locus_tag="ACG86D_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN3141860.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1556 a          903 c          608 g         1894 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat c
//
LOCUS       DAZAJZ010000142         4959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000142 DAZAJZ010000000
VERSION     DAZAJZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.147"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACG86D_002794"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACG86D_002794"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN3141861.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACG86D_002795"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACG86D_002795"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN3141862.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACG86D_002796"
     CDS             2594..3379
                     /locus_tag="ACG86D_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3141863.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACG86D_002797"
     CDS             3392..4243
                     /locus_tag="ACG86D_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN3141864.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACG86D_002798"
     CDS             complement(4367..4819)
                     /locus_tag="ACG86D_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141865.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1678 a          811 c         1036 g         1434 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattc
//
LOCUS       DAZAJZ010000143         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000143 DAZAJZ010000000
VERSION     DAZAJZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.148"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG86D_002799"
     CDS             85..972
                     /locus_tag="ACG86D_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3141866.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG86D_002800"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG86D_002800"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN3141867.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG86D_002801"
     CDS             2274..2720
                     /locus_tag="ACG86D_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3141868.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG86D_002802"
     CDS             2993..4345
                     /locus_tag="ACG86D_002802"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN3141869.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG86D_002803"
     CDS             4516..4803
                     /locus_tag="ACG86D_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3141870.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1696 a          813 c         1029 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gaactgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZAJZ010000144         4806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000144 DAZAJZ010000000
VERSION     DAZAJZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.149"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(523..1026)
                     /locus_tag="ACG86D_002804"
     CDS             complement(523..1026)
                     /locus_tag="ACG86D_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3141871.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1385..2410
                     /locus_tag="ACG86D_002805"
     CDS             1385..2410
                     /locus_tag="ACG86D_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3141872.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2733..3611
                     /locus_tag="ACG86D_002806"
                     /pseudo
     CDS             2733..3611
                     /locus_tag="ACG86D_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            4087..4203
                     /locus_tag="ACG86D_002807"
     CDS             4087..4203
                     /locus_tag="ACG86D_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN3141873.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1604 a          772 c          860 g         1570 t
ORIGIN      
        1 taacatatct cattttacac ttttgttatt ttatacttaa aaaataacga cacagaaaat
       61 accgtgtctc agatttctct actacttcta aaaactactg tttttttcct ttgaaatgcc
      121 ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg agcctaaata
      181 aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt ttatagaaaa
      241 ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt gagatattga
      301 ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt ttgttaaata
      361 aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata atatagtaga
      421 ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg tcatagatac
      481 acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa gacggacagt
      541 tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc ccaaaataat
      601 ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat aggcccataa
      661 acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta cgccaatacc
      721 gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc ctaatttttt
      781 agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa aaaagacaat
      841 caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag tagaaaaagg
      901 gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa atgatagctt
      961 gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt ttgatacata
     1021 ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga aaaatcataa
     1081 aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag cttttttttg
     1141 ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa agttgctatc
     1201 ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc caagttgttt
     1261 actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag gtaatgctga
     1321 caaacgggat tggcatttca tcttggttca gctttttttc caattactag gaaggaggga
     1381 tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat
     1441 gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc atgatttttt
     1501 gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa acataccgat
     1561 gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg ctttcgtttt
     1621 ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt tggttgcttt
     1681 ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct tgttttttgc
     1741 gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac cgatcgtctc
     1801 actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta caaaattact
     1861 ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat ttcacgaaga
     1921 gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt
     1981 cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg catcgatttt
     2041 gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga ttggatcgat
     2101 gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt tgcagaccat
     2161 ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa attatgcagt
     2221 tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc gtggagcata
     2281 cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt tcatcggttt
     2341 aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg ttcgtcaaaa
     2401 attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt tttgtcacaa
     2461 cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt cacacaaaaa
     2521 tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt ttgtttatga
     2581 tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc ttacagatat
     2641 tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta tgtttatacc
     2701 atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg aaaaggggaa
     2761 atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta actcttccca
     2821 aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag gtgtttgagc
     2881 aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag aaaatagaca
     2941 aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa
     3001 gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga
     3061 atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc
     3121 gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt
     3181 acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc
     3241 tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg
     3301 aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca
     3361 cagatacttg gttactatca cccaatctta ctaaatagtt gaaaaaagag tcaaaaatct
     3421 gtctttttgc agccgattat cgattattat cagttgatga acaggacgat agtggagtcc
     3481 cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac
     3541 agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc
     3601 ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa
     3661 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga
     3721 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     3781 ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt
     3841 caataggtaa actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt
     3901 aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc
     3961 tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa
     4021 atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc
     4081 gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc
     4141 aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg
     4201 taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc tattttcaat
     4261 aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat
     4321 gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc agttatatag
     4381 ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag ttatttttca
     4441 attccctttc aacctcttat tttgaccagt agtattgatc actttacctt ttaacacctt
     4501 tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa tcaaaggttt
     4561 tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc aaataaccat
     4621 tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc tcactccatt
     4681 tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa aagttaactt
     4741 taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta ttgctagaat
     4801 aacatc
//
LOCUS       DAZAJZ010000145         4677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000145 DAZAJZ010000000
VERSION     DAZAJZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.150"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..224)
                     /locus_tag="ACG86D_002808"
     tRNA            complement(137..224)
                     /locus_tag="ACG86D_002808"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ser,seq:cga)
     gene            complement(369..935)
                     /locus_tag="ACG86D_002809"
     CDS             complement(369..935)
                     /locus_tag="ACG86D_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3141874.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1190..2521
                     /locus_tag="ACG86D_002810"
     CDS             1190..2521
                     /locus_tag="ACG86D_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFN3141875.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2487..2837
                     /locus_tag="ACG86D_002811"
     CDS             2487..2837
                     /locus_tag="ACG86D_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141876.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2929..3348)
                     /locus_tag="ACG86D_002812"
     CDS             complement(2929..3348)
                     /locus_tag="ACG86D_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN3141877.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(3349..3693)
                     /locus_tag="ACG86D_002813"
     CDS             complement(3349..3693)
                     /locus_tag="ACG86D_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN3141878.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4043..4639
                     /locus_tag="ACG86D_002814"
     CDS             4043..4639
                     /locus_tag="ACG86D_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3141879.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1266 a          868 c          815 g         1728 t
ORIGIN      
        1 aacaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaaatag
       61 gaaaagctat ttttgtttgg ccgttcttat tacttgatgc aggacagctt cttcacaacc
      121 tctaatcgtt gatataagga aggtacagga tttgaacctg cgcgccggta ttagccggtt
      181 cgccggattt cgagtccggt gcattaccac tctgccaacc ttccgtaagt actattttta
      241 aacttttttc acgaaatgtc aagcctattt ctaatagata aataacagaa atcgcttttt
      301 ttagcaaaaa agtccattga cagtttgcag ccaacggact taccgatcaa attcatactg
      361 atagaacact atctgattat ttcaggatta tctaatgcta aaatcgagtc aattgtccgt
      421 ttgatcagtg caatgatttg ttcttctgtt ggatgcatga tgtttgatga catcaatgac
      481 gctaagccag tcactgctac ccatgtacca gtatgcagag catcgacata ttcatccgat
      541 aattgattgt atctttcatc ttctttaact atttcacgga aataattgta tgaaaaatca
      601 tgcattttct ttccgtcccc atgttcatca atgtacaatg ccatgtaaag actatgatgc
      661 ttttttgcaa agtagatata gttcaaaccg agatcaatga ttttatcacc agtacgttca
      721 atagggaaaa catcttcttt tagttgttca aagatagaat ccagcaacgt attttttaaa
      781 tcttccatgt ttttgaattc cagataaatc ggctgtgtcg aaatccccat ctttttagca
      841 atatttctcg cagtaaatcc tttaaaccct tcctttgcaa caacatcata agcagctttt
      901 agaatttgat ccttggtata gacttttctt ctcattaaaa ctcccccact tctttactgc
      961 agtatatgtt gttttattgt accctatttt tagcattttt tgcaaacatt ttactcaaaa
     1021 cgttatattt taaatttttt tcagtcttct ttaacttttt tttgcaaaat taaaaaaatt
     1081 tggttaaatt caatgggttc ccttacattt ttagttttga cctaaatgat agttgtccaa
     1141 atctttcatt tcaagttata gttaaagaaa atgaaaaaga aggtgtgtaa tggaaaacga
     1201 aactgacgtt atttatattc atcctcaaaa acgaatcgtt tcacagaaaa gaaagtattt
     1261 ttatcttggt tttacaggtg ttttttttct ttttattggc ttgctttcta atactcctac
     1321 tgataattgg agtggattgc taactattct tacctcgcca agcaatcttt tgacagatta
     1381 ttttgcttta ggtgttttcg gaagcgcatt cataaatgta gggattttga ccttactttc
     1441 tgtgctttta gcttatcggc acaaggtgat tttaaacggt cccttatttg cttcgattct
     1501 aacagttacc ggtttctctt tttttggcaa aaacctttat aattctatct caatcatttt
     1561 aggtgtgtat ctatacgctg tatttgtcaa taaacctttt tctcagtaca tcatgatcgg
     1621 actgtttggt agcgtattat caccggtcgt tagttatatc acttttggta tgcgttttcc
     1681 tttactgata ggtattttat tggggaattt agctggcata gcaattggta tacttcttcc
     1741 gcccttagct gctcaaactt tagttttcca tcgtggattc acactttata atattggatt
     1801 cacctctggg ttgattgcga tgacttttac tgctgttttg aggttattca gctattcaat
     1861 cgtcgaaaat acattggttt tcaatgaata tcactttcct ctcatttgga ttatttttgg
     1921 ctttttttcg ctgacagtcg gaattggttt ctattataat tcctttcggc tttcggggat
     1981 tcgagaaatt ttcgattcat ctggaaaatt gacaactgat ttcattgcta attctggtat
     2041 tggtgctact ttgatcaaca tgggcttagt tggtctgatg ctgtcttctt atgttttgct
     2101 ggttggcggt cagttaaatg gacctgtcat cggtgctatt ttatctgcgg tgggtttcag
     2161 tgcttttggt tgtcatttaa aaaacagttt tccgatacta gtcggaatat ttattgcttc
     2221 tttgtttgga acttttcatg agatcaccag tactggcatg cttgttgcag ctgtgtttgg
     2281 gacaggtctt gcgcccataa gcggttttta cggcagtttt tacggtgtaa tagctggtgt
     2341 gctacatatt gctcttgtcc ataacgtcag cactttgcac ggaggactaa atctatataa
     2401 cagtggtttt tcaactggtt tcgttgcagg tatccttgta ccgatactag ataactttac
     2461 cgctgtcaga aaggaaaaaa agacacttgg aaaaagaatt atcaaaaaga atcatcgatg
     2521 agcttcttac ttatttatat tcccatgaaa taactgaaat acgtattgga gtagaatttg
     2581 ctgaggaagg gcttttcata gagatacagg gtacggcaga aactcagcca gaagatttag
     2641 atgtacttgc tgatttgctg aacaatcctc gggaccctag tatggaaatc tactatcaag
     2701 aactattagg taatccccga catgatcggg aagattatca tctgttaggt agtttgattg
     2761 atgacgtgga aatcatgtat gcccagccga ttatcaatat taagatcttt cgacgtgctt
     2821 ctcattccta ttattaaccc tattttctat ttataaaaga ctgaatcaaa aaagcagctc
     2881 ctgaaagttc gattaaaatc tactttcagg agctgttcaa gcttgacatt attgattttc
     2941 caatccttca tacacttcat ctgttacagg ttctaaaaac tctgaattac cagcagtgat
     3001 cgccaaatgt tcaaaccaac tgtcttttgt cgcaccatgc caatgtttaa tccctttgtg
     3061 cgttacgacg acatctccag ccattaatcg tcttgctggc tttccttctt cctgatacca
     3121 gccttctcct ccggtcacaa gtaagatttg atagccgtct gtatggacat gccagtgatt
     3181 gcgacaacct ggttcaaaag ttacattacc tacaccaaca gaaacttcgg gatcattcac
     3241 taacatattc agatagcttt gcccggtaaa tacttgagcg taagcttcgt ttttttcacc
     3301 tgctggaaaa atcacgccat tgtgtatttc ttcgtgtttt gtcatcattt acgcctcttt
     3361 cttatcttcg ctattttctt ctaaatagat ttcttttgct aaactaaaag ctgaccaagc
     3421 ttttggccat tctacataaa aagctaaatg ggtaatgatt tctgctattt cttcactagt
     3481 aatgccattt tgcttgccaa tcttcagatg agctggcaat tgttcaaaat ttcctcctga
     3541 aattaatgca gatatggtga tgatactacg ctgatgagca gataattctt ctctggacca
     3601 tacttgtcca aataaaacat catcatttaa ttctgcaaat tttggtgcaa aatctcctaa
     3661 ttgatctcgt cctgcggttt gtttttctgc catttctctt attctccctt cacgactgtt
     3721 aggtgattca actatggttc taaggcaact ttattttcta ttgtttcttc gcatttcatt
     3781 ttaaatgtag tctttcagcc tgtccaatgc ttagtttatt cagtcgatat gctttttaag
     3841 catagttgct aacgcttttt ttattttaaa aaaaacaagt tattaaataa gtctttagtt
     3901 ggatgttttt ttgagatcaa tcttttatac ttatgatgaa cttacaaaat tgtttgtttt
     3961 ctgtcatatc aaaaaatggc gcagttagtt gcttttctag taaagtatag ttaacaaaga
     4021 acctctaagg agttgatgaa taatgaatat tgctgcctct cgaaaactga taaatttcat
     4081 atccattatc ggattaggcc tatcaatcgc gctgacgatt tattttatta atttaggtgt
     4141 tttcaaagat ttgaatgcgt taagaggatt agtaggtgat tccatcattt taggaccgat
     4201 tatttttgtc ttcatccaaa tcctgcaagt agttattcca atcattcctg gagggatcag
     4261 tactgcagct ggtgtcctga tatttggccc ttatgctgga tttatctata actatattgg
     4321 aatctgcatt ggttcgatta tcatttttct gctagggcgt cgttatggaa agccattcat
     4381 actttccatg attagcgata agacatataa caaatatatt ggctggctgg ataatcaaaa
     4441 ccgttttgag aagctgtttg ctctagctat ttttcttcca gttgctcctg acgatgcgtt
     4501 atgtttgatg gctggattga caaacatgtc agtgaaacga tatacactaa tcattttgat
     4561 tgctaaacca ctgtcgattt tcttatatag tatggccttg atttatggag gtcaatatct
     4621 aagtggtctt cttggataat atttattttg taaaattagt cattgattga caatgtt
//
LOCUS       DAZAJZ010000146         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000146 DAZAJZ010000000
VERSION     DAZAJZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.151"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG86D_002815"
     CDS             186..2855
                     /locus_tag="ACG86D_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN3141880.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG86D_002816"
     CDS             complement(2946..3560)
                     /locus_tag="ACG86D_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3141881.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACG86D_002817"
     CDS             3680..4219
                     /locus_tag="ACG86D_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141882.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACG86D_002818"
     CDS             4216..4554
                     /locus_tag="ACG86D_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFN3141883.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAZAJZ010000147         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000147 DAZAJZ010000000
VERSION     DAZAJZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.152"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG86D_002819"
     CDS             127..579
                     /locus_tag="ACG86D_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141884.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG86D_002820"
     CDS             593..1282
                     /locus_tag="ACG86D_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141885.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG86D_002821"
     CDS             1407..3758
                     /locus_tag="ACG86D_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN3141886.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG86D_002822"
     CDS             3838..4233
                     /locus_tag="ACG86D_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141887.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DAZAJZ010000148         4326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000148 DAZAJZ010000000
VERSION     DAZAJZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.153"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1155)
                     /locus_tag="ACG86D_002823"
     CDS             complement(<1..1155)
                     /locus_tag="ACG86D_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3141888.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="ACG86D_002824"
     CDS             complement(1158..3407)
                     /locus_tag="ACG86D_002824"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3141889.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3504..4007)
                     /locus_tag="ACG86D_002825"
     CDS             complement(3504..4007)
                     /locus_tag="ACG86D_002825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141890.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
     gene            complement(4089..>4326)
                     /locus_tag="ACG86D_002826"
     CDS             complement(4089..>4326)
                     /locus_tag="ACG86D_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3141891.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1386 a          792 c          621 g         1527 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaagattgt
     3601 actgttgaga atcctatctt gtcactgatt tgctggattg aatattcagt tgatgataac
     3661 agatcgtttg caatagaaat tttgatttca ctaatatact cttttattga tattcccata
     3721 tcattcttaa acaatcgtga taaataattt tcgtgtatcg atagactgac agcaatatct
     3781 ttaacagata atttttgata cgcattctca cgtattattt caattgtttg acgaacaaaa
     3841 ggattcatct ttttagtaat tatcccttcg ttcagataac tagctgaatt taagaggagt
     3901 tctaaaaatt tataaacttg tttttgtctg gacaattcac tgactagatt gtcctgatct
     3961 ttcagctgag tgttaggttc tgttactaaa tatatagaat tttccatatc caccctccta
     4021 tgttagattt ggataatttt tgttttatta tgtttatatt attacataac ccgtggtgct
     4081 agttaatttt aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga
     4141 cttaatagaa cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca
     4201 gaacgtgagt tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact
     4261 gtttttcttt gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta
     4321 cgtggc
//
LOCUS       DAZAJZ010000149         4282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000149 DAZAJZ010000000
VERSION     DAZAJZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.154"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..520)
                     /locus_tag="ACG86D_002827"
     CDS             complement(35..520)
                     /locus_tag="ACG86D_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFN3141892.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(522..1193)
                     /locus_tag="ACG86D_002828"
     CDS             complement(522..1193)
                     /locus_tag="ACG86D_002828"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141893.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1456..2649)
                     /locus_tag="ACG86D_002829"
     CDS             complement(1456..2649)
                     /locus_tag="ACG86D_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141894.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2733..2933)
                     /locus_tag="ACG86D_002830"
     CDS             complement(2733..2933)
                     /locus_tag="ACG86D_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN3141895.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3357..3590)
                     /locus_tag="ACG86D_002831"
     CDS             complement(3357..3590)
                     /locus_tag="ACG86D_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141896.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3597..4028)
                     /locus_tag="ACG86D_002832"
     CDS             complement(3597..4028)
                     /locus_tag="ACG86D_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3141897.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1057 a         1068 c          832 g         1325 t
ORIGIN      
        1 aacatgtcat tagatagtag acagttccaa ctctttattt accttttttg tatgctgcac
       61 tgtcaatcca agttcctaaa ttgttgtatc catcatcact tatataatca tattggccat
      121 caataatttc gtcaaaccac aaatattctc catctggttt ttgcccaatt attttaatta
      181 ctgattgact agtagttgtc atctggttaa aatcttttat tttacctata ttaacggcca
      241 aaatagcatt tcctcgctta taactctcct ggatttcata tttaacatat tctcgatcaa
      301 gagtttcttt acctatcagc accacagtca ctgatgttcc aattaattga ttatctatcc
      361 agttttttat agcaatatct ccattacgtt taactttctc aaattcagcc ttatctacaa
      421 aaccagcttc ctttgttcct cgcaaagtcc agctgtttct aactaccatt gctcttgtta
      481 catctgcata atggaaacta aaaaaaaccc ttctcaccat attaatcctc ctgaatacta
      541 tttattaaac tgacaatttg ttcaactttt ttatctactt ctccttcagt atctaatttt
      601 tctagcgctt gaaatttact ttttaaggaa tcatcaaaat aatcactgaa attctcttga
      661 atcttatcaa acaaaacctt acttgcacct ttggtaaatc caatcggaac tactatattt
      721 tcattttgaa cagataattc aaattcttca atcattccat ctgctgtaac tatttcacct
      781 gattcaagtt tgtttccaaa aacaaaaata ctaatcccag actctgcaat aatgttttct
      841 cgatatcttc tccactcatg aggcatatca aggggaagtg gtcttaaaga aagtacgtct
      901 tgtatgttat tttttcttaa atttcttctc tgctttagaa ctcctgaaac aatactatta
      961 ccaattccaa gtccgtaacc tgatactatt ctataaccgg attcagaaag cctatatgct
     1021 aatttctcga caaattttaa tcctttcgat tcagatgtaa atggagtata atcaacagca
     1081 cttcccgaaa taaaaatagt ttttctcaaa tacttttttc taatactttt caaaatatca
     1141 gtaatctcag aataatcatc tacttcaaca acgtttatcg aaaatctgga caaatcattg
     1201 atttgtagtc ttctttttgt cagttcataa ttatatctct cttgatcaaa aaaatcctca
     1261 gaatcgacaa aattttcctt taaaattata tgggatttta gattatcttt ttagaagttc
     1321 ttgaatgtcg gtatagcccg tgtttccggg cttttccggc atttttgata ttgttagata
     1381 cttacaaatg ttcttattac ctctcaccat ttccccctca aaagtagtaa atggagtagt
     1441 aaaaccggcc ttgtgtcagg ctgccagccg ttccatttct gcccttgcgg aatcgaatgt
     1501 ggcgtgtgca taatagttca gcgtcatggt gatattggaa tgtcccataa tgtactgcaa
     1561 ggctttgggg ttcattcccg cattggctaa ccgggtgcag aatgtatgac gcatagcatg
     1621 gggcgtcgtc gttttcggca acgcttcttc atggctcttg ttgtacttct tcacaagcct
     1681 gccaaacatc ccgccataat ctaccgccgt catgggatag ccgttctgtt tgagaaacag
     1741 gaaattggca tacccgtcaa taatgaacgg ctttgcacct ttgcggtttt ttatcactcg
     1801 ctggaacgcc tgatacactt cctcattcat ggggattttt ctgacgccgc tttgggtctt
     1861 gggttcgtca atatagtaac cgacgccggt atttctcaaa agctggtggc tcacgatgat
     1921 tacccggttt tcaaaatcca aatctttgtc cgtcagtccg cacagttccg aaatgcgaag
     1981 ccctgtcccc agcagaataa cgattgcgtc ataatgcttt tggtaaacct tatcaccttg
     2041 aataaaggac agaaaacttt cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc
     2101 gtcgtccaga acgtcactaa gctggaagtc aaacgggttt tttcggatgc aatcgtcctg
     2161 tatggcggtg taaaaagccg ctttcaggga acgcttgtcg ttattgatgg tcttataaga
     2221 aaggcccttt tctttcatcc gaagcgccca ttcctttgcg tcggacagct tcacactgtc
     2281 aatcgggcag ctccccagcc gatcttgttc cagaatacgc atcagccgtt cccggcccag
     2341 tttggtgttg tgcttcacat tgccccggtg cctgatctgc ttggcgtaga gctggcagac
     2401 ggtcattttc ttgccgatgg tgtctatccc gtcgtcaagg tctttctgga tttctttctc
     2461 cttttcccgg agtgaaatat cctcccgttt acctgccggg gtcttatccg taggcactaa
     2521 cttccatgag taaacaaact gcggttttcc aaaggtatca acatatttgt aagcatatcg
     2581 tccgtctttt ctctggctct ctccagaccg caaaacgcga tttttgctgt ctcgtctttt
     2641 ttctgacatc tcgtcatgct cctttccatg acggaaagag ccgcatatat gcttataaat
     2701 tattataaca tatctgcggc cctttttcac tattagattg tgtccaaagt gtcaattatt
     2761 ttctcaaact gcttgcgttt gatctggatg cggttgccat tcataatgac ccagccagag
     2821 gccggatttt cctcggccag cttacgcaat ttgttttctc cgatacggaa atatttagac
     2881 gcttcctcaa tcgtcagcgt gtatttttcc caaataggca catcatggtt cataatgcag
     2941 ccccctttca caaattcaaa ggtttgtgtg atttaggggc atagacggac ggctgctggc
     3001 acagctccac gggactttca cccccgcgtg gttctcacgc ggccctaccc attgcctgcg
     3061 acgcttttaa cgctcggact gtggctataa gggagtatca tagtcctgcc tgtacgtcgt
     3121 cgcccgcaag ccgccacgct cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta
     3181 gggaaaagta tcatggcgca ccagccgtcc ggctcggcac agacagaatg tgtccgtatg
     3241 ccctatgatg cgccgccgtt atcttgcggg catatttatc aaggtgcgat caggccaccg
     3301 ggaaaacacc gaaacgggga aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag
     3361 ttcgtgacga tagcgtggat gagcttgatt tccagacggc gcttcatggc ctcgtccaga
     3421 cacacatgcg gatagccgtt ttcgtcatag agcgtccggg tacacagctt gttcatgtaa
     3481 ccgctgtaat actgcaaaac gcgctctacg gctccggcat ccccagcgcg ggcggcctcg
     3541 atcaccgaaa ggggcaaaag ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa
     3601 cctcccttcg gcatcaacgc ctttagctgt ttccgcagct ccgataaggt atttgtcctg
     3661 tgccgctgaa cggcactccg ggacattccc acaaggccgc cgatctcacc atctgccata
     3721 tccagaacac agtgcaggat taaaatactc tgctccatgc tgggcagcgc ggcaaaggct
     3781 tcggcaacaa gctcattgtt gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg
     3841 tcgctggggt agtgatctac tgtacagagc ttgtccaaat ctgcctgtga caaagagctt
     3901 agtgacactt cacggtcacg gcggcgcttc atttcagcca agtagctttt ggcctcgtta
     3961 cgcagtaccg tcttgcaaaa agcatcgaac atgcaccgcg cttcatagtc gcgggggata
     4021 gcatccatct tctcaccccc tttctgtggg ggacttaggt ggttctctcc ctttccgcta
     4081 acaagacttt gacaaggccg ccagctaccc gctaaatacc tcctttttga aaatttctca
     4141 aaaaattttt ttggggacag ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc
     4201 aaacctaccg ggcgagaaaa tgctttgcta tgctgctgat ttacatggta tagtacatac
     4261 tcataaccgg aatagccctg tg
//
LOCUS       DAZAJZ010000150         4262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000150 DAZAJZ010000000
VERSION     DAZAJZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.155"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..767
                     /locus_tag="ACG86D_002833"
     CDS             66..767
                     /locus_tag="ACG86D_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3141898.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1339..2355)
                     /gene="tsaD"
                     /locus_tag="ACG86D_002834"
     CDS             complement(1339..2355)
                     /gene="tsaD"
                     /locus_tag="ACG86D_002834"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN3141899.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2352..2816)
                     /gene="rimI"
                     /locus_tag="ACG86D_002835"
     CDS             complement(2352..2816)
                     /gene="rimI"
                     /locus_tag="ACG86D_002835"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN3141900.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2823..3365)
                     /gene="rimI"
                     /locus_tag="ACG86D_002836"
     CDS             complement(2823..3365)
                     /gene="rimI"
                     /locus_tag="ACG86D_002836"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN3141901.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3349..4071)
                     /gene="tsaB"
                     /locus_tag="ACG86D_002837"
     CDS             complement(3349..4071)
                     /gene="tsaB"
                     /locus_tag="ACG86D_002837"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN3141902.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1259 a          914 c          735 g         1354 t
ORIGIN      
        1 aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact
       61 ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt
      121 ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt
      181 ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga
      241 ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact
      301 atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt
      361 atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa
      421 aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag
      481 ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa
      541 aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca
      601 ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa
      661 ctgctggaca agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc
      721 gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat
      781 aagcagtgac cctgatattt atcagggtca ctgcttattt tcctccctaa aacttctgaa
      841 agatccggca gttttagatc actgggcaag actcaaagat tttctatgtg tgtttcagtt
      901 atgtaatatt cactgtttgc aaaaaatact gatagattaa ctagaggaag cgtcttgtcc
      961 tcatccgacc tagattattc gatattcctc gacaataaac gattaatcag tgagggcaat
     1021 cagaggaacg gataatgatg aataagctgt aaaaaatagg gctttgacaa aagcgtgtca
     1081 cagcccttat ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg
     1141 ttcataaaca tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca
     1201 ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag
     1261 ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc
     1321 agatttttca caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata
     1381 gctagcaaaa tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt
     1441 atctccacaa agtcgaagag ggggaatcac tagttctacc tcagggagtt tagttgccaa
     1501 agcatcctgc aatccttcac gcaacccttg attggccgct acacctccag ctacaatcag
     1561 ttgcttgact ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac
     1621 acttgcttga aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc
     1681 attatgcacc aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc
     1741 ttcatggatc atcgctcgtg gaaaatggta attatctttt ccctgatgag ctagctgatc
     1801 gatttctttt ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc
     1861 tcctgctgcg tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata
     1921 gactaattct gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt
     1981 aaccagacgg gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg
     2041 gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc
     2101 tggtccataa gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag
     2161 tgcatcttcc aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggaac
     2221 tacgccacca aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc
     2281 tgttccattt ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat
     2341 caattcttca ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct
     2401 tccgtcggat tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca
     2461 taaagttttc ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct
     2521 tttatttgtt ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct
     2581 gaaactgcaa cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata
     2641 aagccacaga tctggttatc ttcaactaaa agcagataat cagagtgagg ctgttggatg
     2701 tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc
     2761 atccatacgc attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa
     2821 tctcactcat tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa
     2881 tatctttttt tgatcgttgt tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct
     2941 tgattactca tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc
     3001 tcgatttcgt caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca
     3061 atattcgtga tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt
     3121 ccattatcaa agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag
     3181 gcactttttg tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct
     3241 ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgattgtt
     3301 ttttctgtat aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc
     3361 aacataactt tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg
     3421 ttttaaatag cgaggaagaa aagcttgttt ctctgcaact ggttcagcta aagcacctaa
     3481 ttgagccaca gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact
     3541 aggcaatatt tcctttattt catagaagaa tttgtgacaa tcactcccga cgaaataaat
     3601 agcttcattc agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc
     3661 aagtacaatt tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct
     3721 tcgagcatca aacatgggca caataatttt atccacaccg atgcaattag ccgcgatggt
     3781 tttcagacta gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt
     3841 cgttacacct atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc
     3901 aatgtctttt ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt
     3961 tacactgtgg ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac
     4021 cacaccgatc gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat
     4081 cctttctgct atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa
     4141 aataccaaag gcaaaactag tacaatgata catgattatc tgctggcggg caaattttgc
     4201 cccttcctga aacacaaaaa agctgcggta tcacttcatt tccccacagc ttttccatct
     4261 tt
//
LOCUS       DAZAJZ010000151         4196 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000151 DAZAJZ010000000
VERSION     DAZAJZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.156"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..242)
                     /locus_tag="ACG86D_002838"
     CDS             complement(<1..242)
                     /locus_tag="ACG86D_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141903.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRE"
     gene            complement(573..806)
                     /locus_tag="ACG86D_002839"
     CDS             complement(573..806)
                     /locus_tag="ACG86D_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN3141904.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(821..1771)
                     /locus_tag="ACG86D_002840"
     CDS             complement(821..1771)
                     /locus_tag="ACG86D_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN3141905.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1768..2610)
                     /locus_tag="ACG86D_002841"
     CDS             complement(1768..2610)
                     /locus_tag="ACG86D_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN3141906.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2612..3331)
                     /locus_tag="ACG86D_002842"
     CDS             complement(2612..3331)
                     /locus_tag="ACG86D_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3141907.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            3879..4181
                     /locus_tag="ACG86D_002843"
     CDS             3879..4181
                     /locus_tag="ACG86D_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141908.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         1403 a          760 c          534 g         1499 t
ORIGIN      
        1 ttctctctcc aacttttctc tatatctata ttttctaagt ttaagaggta tttggtaaac
       61 tcttcattat acatattaat aagcgcatta atatgcggct gaataatttc ttcaaataac
      121 gcttccttag aagtaaaatg tttataaaaa gcacccgtag ttacgcctgc ggtcaggcac
      181 agttgcctta tcttagtttg ctgaaatcct tctttttcaa aatggcttag tccactttct
      241 attaactttt ggtgagttat aatataatct ctggtttcta tgggtacttc ctcctaataa
      301 gatgaattaa ctttattatc ttacacagga cttgaagttt caatcgttaa cgtttattac
      361 tgaactcatt ccttagagct aaactaatga gaaagagcag aataactgat acaaaaagca
      421 atcctacaag aattaataga ctcatctaaa tcattcacct tttctaaaat aatccgagaa
      481 agatttaaaa aaattatatc cagctattag gataagtcct atcataatca ctccaatagc
      541 cattttctta tcctccctaa aaataaagtc ttttacttaa aacctttttt gtaatcgtca
      601 ataaattttt tccctttatc agttaattga aatcctatat ctaaatagtc ctcatatcta
      661 agtttagcca gcatctcact tacagatgct ggactaacac ttagaacttt tgctaaatca
      721 ttatttttta atttcttttt tctttttgat aattcctgaa ttgctagtaa ataaattagt
      781 atagtagtcg ttttaatttg taccatacct accctccaaa ttatttcgaa aggccttcag
      841 caattttatc caggttccat ttcatcatcg catagtaact atcaccatct tgtccttttt
      901 ctgcaattga atcagtaaaa atcgttgaat agataggaat attggtatct tttgatactg
      961 ttttcattgg tctatcatcc acactgcttt ctacgaataa ggagggaact tttgttgtgc
     1021 gtaatttttc aactaagtgt tttatttgat ctggtgttcc ttcttcttca gtgttgattt
     1081 cccaaatgta agcagaaggc acattatacg ctttcgagaa atatttaaag catccttcac
     1141 ttgtaacaat cattttttta tcattcggaa ttgaagcaaa tttagattta gcttctttgt
     1201 ctagagaatc caatttttca atatacttat ctagattttc tttatagaat tttttattat
     1261 caggatcttt ttccgctaac tgtttttcaa tatttttagc gtaaataata ccgttttcta
     1321 aatttaacca agcatgagga tcttccttcc ctttctcact ctgaccctct aagtaaataa
     1381 catctatgcc atcacttgct gcaaaatagt ctttgttttc ctctttgttc gcatttttaa
     1441 ctaattttgt aaaccaagca tttcctccag tttccaagtt aacaccgtta taaaaaatca
     1501 aatctgcttt tgaagttttt tgaacatctt caggcaaagg ttcatattcg tggggatctt
     1561 tcccaatagg tacgatactg tgtaaatcaa ttttatcttt tgctatattt tcggtaatat
     1621 ctgctaaaat cgaattagta actactactt ttaatttttc gttcgaagcc actttatctt
     1681 tgtctttact atttattgaa catgccccga atataaaaag agcaatcaaa atccccagac
     1741 ttcccagtac tgctttccat tttctcatca taacgatctc tccttctttc ttaaaaattt
     1801 ttgttttggt gagattagaa aactaattac aaataagaat gccgctgtta aaacaatact
     1861 tgatcctgct gctatgttaa aactataacc aataaacaat ccaagcaatg atgaacttgc
     1921 tccgactgtt gcagaaataa atatcatagt tttcaagcta tttgcatata aatatgctgt
     1981 agctgctggt gtaattaaca ttgcaacaat taatatagtt cccacacttt gcatagctgt
     2041 tactgctact aaagttaata gaaccatcaa tagataatga taaaaactga cattcatccc
     2101 tattgctttt gccattaatg gatcaaaaga agtgattaat aattctttaa aaaatagaac
     2161 tactataacc aacactaaaa ccgaaacgcc aattgtaatc cacttatcaa tatcttgtac
     2221 agctaaaata tttccaaata aaatatgaaa taaatccgtt gagctatttg caactcctat
     2281 aaggataact cctagtgcta aaaaagaact gaaagtaata ccaattgctg tatctccttt
     2341 tattacacta ttgcttttta taaaagtaat aatgatagaa gctaatagac caaatacaat
     2401 cgcaccaata aaaaaattaa tacctaaaat ataagaaatt gctactcctg gcaaaacagc
     2461 atgagaaata gcatcaccca taagagacat tcctcttaaa ataataaaac aacctactgt
     2521 tccagaaaca atccctataa ctacagatgt aattagagca ttctgtaaaa agtgaaagtc
     2581 atacagacca ttaataaaat tttgaagcat attattcccc ctctccaata aatatagtat
     2641 ctccataggc tttttttaaa ttttcttcat taaatacaga ttctacactt ccgtgagcaa
     2701 ttagatttcg attcaaaaga ataatattat caaaatactt ctttaccttg cttaaatcat
     2761 gatgcacaat tagcactgtt tttccctgat gtttcaattt ttttaacgta tccataatta
     2821 ttcgttcact aactaagtct atccctacaa atggttcatc taaaaatatg aaatccgcat
     2881 tttgagcaag acatcttgct aaaagtacac gttgaaattg tcctccagat aattctccta
     2941 tttgatgatt agaatatttt cccatatcga ccttttccaa agcctttgaa actctttgcc
     3001 attctgcatt tttaattctt tgaaaaactt tcattccggc atatgttccc atagatacac
     3061 attcttttac ttttattgga aaagtaaaat caatatcagc tttttgctct acatatgcaa
     3121 tcttcaccag ttttttattg atatcttcat tattaatgga gacctctccc tgatgaggga
     3181 taatatttaa cattgcttta agcaatgtgg attttccagc accatttgga ccaataatac
     3241 ctgttaaagc accttcttgt atttttaaag aaatctcgtt caatgctaaa aaatcgttat
     3301 aacaaacatt aagctttttc acttctaaca taactttctc cttctaaatg ataatgatta
     3361 tcacgatcat atggtataca aaaattatat tttagtcaac cctaaatttt atagtttaga
     3421 tttttttctg ttgctgaaga ttagaatgac aaaactattt gaaactacgc gtatagtaag
     3481 tagctttcaa aatatataaa aaaggagcgt tctcaaatga gaacatgctc ctaaaactac
     3541 tttattggcg tatggaaaaa ttatacttga agtggatagc gggttgaata agttgataag
     3601 catcaaaaca tttataaaaa ttattcaaga ctatgtctgt tccatataca aatatgaaaa
     3661 tcagtaaact ccatttaact tttttcgtag tgtgtctttt tatacatgtt tatcgttatt
     3721 aatagtgaat aggaaaaaaa ctatttttga tcattgacaa ttaaatacaa tttttgaata
     3781 acgatccctg ctattcgagc tagaaactca agtacgccat gacctgatta gcgacaaata
     3841 cttagagatt tctttttgtt ctgggaaaac aaaatgccat gacaatagca gcaaataatg
     3901 atacttccgt aaggctcgcc gtaaactggg gagaggtgaa attcctatgt gcttacccaa
     3961 agcaattcaa aaatttcata actactattt taggagatga ttttatagaa tccagagtct
     4021 acctgaataa aaaacaaacc tgtgcatttc taggaatcga taataatact ctagataagt
     4081 ggattcgtat atataatttc ccagtaataa gagttggtgg tgtagttcgc tttgacgtag
     4141 aagatattaa agactttatg aatacattta aaataacttg aaattttctt ctcgcc
//
LOCUS       DAZAJZ010000152         4104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000152 DAZAJZ010000000
VERSION     DAZAJZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.157"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..1719)
                     /locus_tag="ACG86D_002844"
     CDS             complement(214..1719)
                     /locus_tag="ACG86D_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3141909.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1716..2402)
                     /locus_tag="ACG86D_002845"
     CDS             complement(1716..2402)
                     /locus_tag="ACG86D_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3141910.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2595..4016)
                     /gene="gndA"
                     /locus_tag="ACG86D_002846"
     CDS             complement(2595..4016)
                     /gene="gndA"
                     /locus_tag="ACG86D_002846"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN3141911.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1129 a          864 c          696 g         1415 t
ORIGIN      
        1 tccctatttt tatttattat ttttaaacac gaatattaca gcattttatt gttttttcgt
       61 tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt gttcgctagt
      121 ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct tattctttgt
      181 tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct ttgcgatagg
      241 aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt
      301 atagttttca accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct
      361 agctcttgct ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg
      421 tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga
      481 gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat
      541 taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc
      601 taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac
      661 ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa
      721 gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc tggcgcttaa
      781 tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc
      841 ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg
      901 ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc
      961 caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat
     1021 agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa
     1081 acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag
     1141 cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt
     1201 ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt ttccttctag
     1261 tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt
     1321 tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag
     1381 ctcagctata aagggatcga tcttcattaa gtttccttcg accagtgaag tatgatctac
     1441 gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag
     1501 ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt
     1561 tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt
     1621 aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc
     1681 tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt
     1741 ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa
     1801 cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt
     1861 agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg
     1921 tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt
     1981 tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca
     2041 atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt
     2101 ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct
     2161 cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct
     2221 aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc attatttaat
     2281 gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc
     2341 aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc
     2401 atttattatt ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat
     2461 aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa
     2521 gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa
     2581 cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg
     2641 acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc
     2701 agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt
     2761 acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg
     2821 atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca
     2881 tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg
     2941 atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat
     3001 ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat
     3061 tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt
     3121 acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga
     3181 agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca
     3241 gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc
     3301 agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta
     3361 aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat
     3421 tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt
     3481 ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc ttttgcagaa
     3541 atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt
     3601 gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg
     3661 ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg
     3721 tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt tgcatctgtt
     3781 gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat
     3841 tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt
     3901 gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag
     3961 gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga
     4021 actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca
     4081 ttgcaaacta aagaacaaca tatt
//
LOCUS       DAZAJZ010000153         4098 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000153 DAZAJZ010000000
VERSION     DAZAJZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.158"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1625
                     /locus_tag="ACG86D_002847"
     CDS             201..1625
                     /locus_tag="ACG86D_002847"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN3141912.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            1711..2139
                     /locus_tag="ACG86D_002848"
     CDS             1711..2139
                     /locus_tag="ACG86D_002848"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFN3141913.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            2227..3393
                     /locus_tag="ACG86D_002849"
     CDS             2227..3393
                     /locus_tag="ACG86D_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN3141914.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1379 a          544 c          767 g         1408 t
ORIGIN      
        1 ggattctgac tgccaatatt gattgatgag attgaagaaa tatatagttg atcatggata
       61 tctttatttg aaaagatctt agctattcac atattcaaaa aaatttttac tacgaggact
      121 cacacgatac agaggaattt aggtagttct aattaaacca gaaagtcatc tatagatgtg
      181 agttgaggga ggtcatacgg ttggatagta gcagtaataa atcacattca atagctaaaa
      241 aagcttttat ttacgcaata ggaacattat catcaaaaat gatatctgtc attataatcc
      301 ctatctatgc agtatatatt tctgttgccg atttaggaag ctttgattat caaaatacaa
      361 ttgctcaatt tctttctcca gttatttttg gagcaatatg ggaagcaatt ttacgcatgg
      421 gattaggcaa agaaaaaaat attcagaaaa aagtagtgac gaacagtatt tgttttgtta
      481 tagcaaatac agtgatatta gccatttttt taccaataat atatagaatg attttcccga
      541 atttagagta ttatcaattg tttgtttttc aattaatttt cactcctatc ctaagtgttt
      601 ttcaatatat tgtaagagca ctaggtgaat caaagatttt tgtgatgagt ggtataatcg
      661 ctgcgggtat caacctgtta ctccttacta tttttgttgt tacaatgggt atggggatac
      721 ttggtctctt actctctatc ataatttcta atgttataaa tttgattttt ttgattttca
      781 gggggcagat ctttcaatat ttcgcaacga gttcttttga ttttttattt ttaaagtcca
      841 tgctgaaata ctctatccca ctcactttga atctcacttt tggatggcta atagttggtt
      901 tttcaagatt ttatattgga acgtacatcg gagtaacaga aaatggtatt ttttcttttg
      961 cgatgaaatt ttcatctata attttaactc ttacaagtat tgttagtatg tctatgatag
     1021 aggatgcggt tatcacgtct gagaatgatg atttcatcga aagattcgtt gagaaaaata
     1081 cctatatttt taaaatgtac attaggttag cgactctttc tattcctatc atttgctgtg
     1141 catactcttt tataaccaac cctgattata aaaaatcatt agttctagtt ccaatattag
     1201 ttattatggc atgtttcagt gggatgacaa caaatatcgg aaatatattt gcagtataca
     1261 ataaaacaaa tcagttatta atgatttcag tagtgtcagg tctattgagt gttgttggca
     1321 cctatttatt aggctattct tttggagtaa ttggtgtctc atttgctact ttgctcggta
     1381 ctttagtatt atttggactt agatattatt ttggaaggaa aattgcttat tatgcatttg
     1441 attggaagcg aatatcgcta cttttagttc tctattccgc aatcgctttt gtatgcattc
     1501 atagaggctt ctattttaat cttttcttaa cgattatttt tagtgctatt ttcgttttta
     1561 cgtataaaca attaattata acgtatctaa aaaaattttt cagaataaaa atggctaaaa
     1621 ggtgagggta tacgaagtaa gaatagaatc tttcgaagaa taaagagaaa actatttgga
     1681 aaaaatctgc gagaatgtat taatgaaggt ttgatattcg gaaaaaaaca tagtttagta
     1741 gaggttcctg aatttgggag cgagccatat ttaattgaaa taggggataa tttcagatgt
     1801 tcatttggtg ttaaatttat aacacacgat ggtggagtct gggtcttaag gaagatgagt
     1861 tctgtgagcg agtcaacttc aaaaataggt aggatcaaat tgggaaataa tatttttatt
     1921 ggtatttact ctattgtttt cccaggagtt tcaattggcg ataatgtaat tattagagcg
     1981 aacactttag taaataaaga tatcccaaat aatagtgttg tagcgggtag ttcatgtaaa
     2041 gtgatttcaa ctatagagga gcattatgag aaaaataaaa atgaatttat taaatatcga
     2101 acctcgatga ttttgaagca aaaaaatttt ttctattaaa taatattgat tgaataagga
     2161 atatgttcaa aaggataaca gctaaaatac tataaaatga aatttgattg gagcgtatgg
     2221 aagaatatga aaatttcagt tttcggatta ggctatgttg gtctagcaaa ttcactatta
     2281 ttagcacaaa aagagcaggt caaagcttat gatatcgtgg aagaaaaaat cacgatgtta
     2341 caaaatggca tctcaccaat agaagataaa gaagttcatg aatttttaaa acgggatgac
     2401 ttaaatgtcg aatttacgtc aaattttact gatgcagtaa acttcggtga ttacttgatt
     2461 attgcaactc caacagatta tgacgaaaag aaaaactatt ttaatacatc aacagtagaa
     2521 gatgtaattg agaaggcttt agcaattcgt ccagattcaa cattcattat taagtcgaca
     2581 gttccagttg gctatgcaaa tgaaattaaa gcgaaatttg gtacagaaaa catcattttt
     2641 tcacctgaat ttttgcgtga aggaaaagct ttgtacgaca acttatatcc atcaagaatt
     2701 atagttggag aatgttctgc tcgtggtcgc gagattgcaa ctatgtttca aagaaacgca
     2761 tttaacaaag atgcatcagt tcttttgact cactcaactg aagcagaagc tattaaatta
     2821 ttctcgaata catatttggc attgcgagtg gcgtatttca atgaattaga tacgtatgca
     2881 gaatttcgtg ggcttgatag taagcaaatt attgaaggtg tcggtttgga tccacggatc
     2941 ggttcgcatt ataataatcc ttcttttggc tatggtggat actgtttacc caaagacaca
     3001 aaacaattaa gagccaacta tgaaaaagta ccaagcaata tcatcagtgc aattgttgat
     3061 gcaaatacaa caaggaaaga ttttattgct ggacagattt tggcgaaaaa accaaatgtt
     3121 gttggaatct atcgtttaac gatgaaggcc ggttcagata attttcgata ttcgtcgatc
     3181 caaggaatta tgaaacggat taaagccaaa ggaattgaag tggttgttta cgagcctaca
     3241 ttggatgaag atgaatttta tcattcacaa gtattgaaag atattaatga atttaaaaat
     3301 atttctgatg tgatcgtatc taatcggttt gaagatattt taaaggatgt tgaagaaaaa
     3361 gtttatacca gagatatctt tggtgataac taaataataa tgaacgggta catggaggaa
     3421 aatattttag atggtgactc gaaataaatg tttgttctca ttgcatctgt atctataccg
     3481 ataagtttaa aaattccgat taggaaaatg aagttggcta tccttgcgag gaatttattg
     3541 ctggattatt ttatgaacta aagcaagaca tcatcagaaa aaaatccaaa atttgacaat
     3601 gggattgatc gtttaccagc tgtaagcgaa gtatctgaca cgaaaatgga aaatgcaatt
     3661 actgttatta ccaatagctt tttttcatcg atcaagtatt tgaaaacatt atttgtttgc
     3721 caagctgatc aatgttcttt cagacttgga agaacattga tcagctcaca agtaatgatt
     3781 gaagtttagg ttatgggata aaggataagt gaaataaaag tcgtgtctgc atctttgtta
     3841 gtaccagtaa atttttctac tacggcgcta gtcacgacag tccaggcatc tgaaattgaa
     3901 gatgcaaata gagtttctgc tagtttaggt attgatgtta ctgtattagc attaatctta
     3961 gagccagtag catccaatag gtgtagctat agttctaata gtataaaaca atgcagagga
     4021 tatgattgct ttatgtctcg gtgtgtcagg tcaaaataga gttccatatg gggcgatttc
     4081 tcaatgaata atcttata
//
LOCUS       DAZAJZ010000154         4071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000154 DAZAJZ010000000
VERSION     DAZAJZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.159"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACG86D_002850"
     CDS             85..1434
                     /locus_tag="ACG86D_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3141915.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACG86D_002851"
     CDS             complement(1562..2152)
                     /locus_tag="ACG86D_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN3141916.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG86D_002852"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG86D_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN3141917.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          745 c          789 g         1362 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaac t
//
LOCUS       DAZAJZ010000155         3965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000155 DAZAJZ010000000
VERSION     DAZAJZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.160"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACG86D_002853"
     CDS             2..460
                     /locus_tag="ACG86D_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141918.1"
                     /translation="MHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVL
                     LSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLII
                     FITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(586..1263)
                     /gene="nth"
                     /locus_tag="ACG86D_002854"
                     /pseudo
     CDS             complement(586..1263)
                     /gene="nth"
                     /locus_tag="ACG86D_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1270..1968)
                     /locus_tag="ACG86D_002855"
     CDS             complement(1270..1968)
                     /locus_tag="ACG86D_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN3141919.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2236..3420
                     /locus_tag="ACG86D_002856"
     CDS             2236..3420
                     /locus_tag="ACG86D_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN3141920.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3426..3869)
                     /locus_tag="ACG86D_002857"
     CDS             complement(3426..3869)
                     /locus_tag="ACG86D_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN3141921.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1039 a          780 c          645 g         1501 t
ORIGIN      
        1 tctgcaccat ctttttttat cgaaaacaat acctcgttat tccaagaata aaaaagctga
       61 aaaattttgt ttattattaa ataaatttac tttaattgct cctatccttg catttattat
      121 tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa gctctcatgc
      181 aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc tgatgagcct
      241 gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt tattatcact
      301 tatcattttt attacacctt tagatagata cgtcacttat ttatataatc ctttggaata
      361 ctggacttat tttataggta tattggaagg cattattttc tacatcgggt attttcccaa
      421 taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac
      481 tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa
      541 taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt
      601 cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag
      661 caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatcaac caaaaaaaat
      721 cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc
      781 tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac
      841 gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt
      901 ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc
      961 gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa
     1021 accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg
     1081 tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg
     1141 ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg
     1201 tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa
     1261 catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg
     1321 agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc
     1381 cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta
     1441 attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg
     1501 ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg
     1561 gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt
     1621 ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta
     1681 aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc
     1741 atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat
     1801 agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag
     1861 aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt
     1921 agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc
     1981 gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt
     2041 acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt
     2101 tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa
     2161 tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca
     2221 taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc
     2281 tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg
     2341 ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt
     2401 tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg
     2461 cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt
     2521 ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga
     2581 gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa
     2641 gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg
     2701 ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc
     2761 ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca
     2821 gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt
     2881 tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt
     2941 caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg gttggttggt
     3001 ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga
     3061 ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc
     3121 gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg
     3181 attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc
     3241 aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct
     3301 ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt
     3361 ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag
     3421 tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg
     3481 tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga
     3541 tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt
     3601 tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt
     3661 gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta
     3721 ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca
     3781 aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa atccagatat
     3841 tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg
     3901 tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc aatctgaaac
     3961 caaga
//
LOCUS       DAZAJZ010000156         3878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000156 DAZAJZ010000000
VERSION     DAZAJZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.162"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACG86D_002858"
     CDS             complement(718..3867)
                     /locus_tag="ACG86D_002858"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFN3141922.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAZAJZ010000157         3816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000157 DAZAJZ010000000
VERSION     DAZAJZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.163"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG86D_002859"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG86D_002859"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN3141923.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACG86D_002860"
     CDS             complement(2262..2513)
                     /locus_tag="ACG86D_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN3141924.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACG86D_002861"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACG86D_002861"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN3141925.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACG86D_002862"
     CDS             3463..3681
                     /locus_tag="ACG86D_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141926.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1055 a          813 c          710 g         1238 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcgga
//
LOCUS       DAZAJZ010000158         3756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000158 DAZAJZ010000000
VERSION     DAZAJZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.165"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG86D_002863"
     CDS             <1..1087
                     /locus_tag="ACG86D_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141927.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACG86D_002864"
     CDS             complement(1305..2447)
                     /locus_tag="ACG86D_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141928.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACG86D_002865"
     CDS             complement(2452..2637)
                     /locus_tag="ACG86D_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3141929.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACG86D_002866"
     CDS             complement(2991..3236)
                     /locus_tag="ACG86D_002866"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3141930.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACG86D_002867"
     CDS             complement(3230..3631)
                     /locus_tag="ACG86D_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3141931.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1241 a          678 c          567 g         1270 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaa
//
LOCUS       DAZAJZ010000159         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000159 DAZAJZ010000000
VERSION     DAZAJZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.166"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACG86D_002868"
     CDS             complement(<1..353)
                     /locus_tag="ACG86D_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141932.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACG86D_002869"
     CDS             complement(613..1161)
                     /locus_tag="ACG86D_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141933.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACG86D_002870"
     CDS             complement(1162..2019)
                     /locus_tag="ACG86D_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN3141934.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACG86D_002871"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACG86D_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACG86D_002872"
     CDS             complement(2581..2910)
                     /locus_tag="ACG86D_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3141935.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACG86D_002873"
     CDS             complement(2987..3259)
                     /locus_tag="ACG86D_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3141936.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACG86D_002874"
     CDS             complement(3259..3522)
                     /locus_tag="ACG86D_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3141937.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAZAJZ010000160         3717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000160 DAZAJZ010000000
VERSION     DAZAJZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.167"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACG86D_002875"
     CDS             72..155
                     /locus_tag="ACG86D_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN3141938.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACG86D_002876"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACG86D_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN3141939.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACG86D_002877"
     CDS             complement(1280..2143)
                     /locus_tag="ACG86D_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3141940.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACG86D_002878"
     CDS             complement(2145..2417)
                     /locus_tag="ACG86D_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3141941.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACG86D_002879"
     CDS             complement(2434..2643)
                     /locus_tag="ACG86D_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN3141942.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACG86D_002880"
     CDS             complement(2721..3617)
                     /locus_tag="ACG86D_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3141943.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAZAJZ010000161         3696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000161 DAZAJZ010000000
VERSION     DAZAJZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.168"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1190)
                     /gene="mnmA"
                     /locus_tag="ACG86D_002881"
     CDS             complement(69..1190)
                     /gene="mnmA"
                     /locus_tag="ACG86D_002881"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN3141944.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            1594..2193
                     /locus_tag="ACG86D_002882"
     CDS             1594..2193
                     /locus_tag="ACG86D_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3141945.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2331..3617)
                     /gene="efmA"
                     /locus_tag="ACG86D_002883"
     CDS             complement(2331..3617)
                     /gene="efmA"
                     /locus_tag="ACG86D_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN3141946.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT         1128 a          818 c          648 g         1102 t
ORIGIN      
        1 gctatgctat ccaaaagaaa aggtgtttct tttgttagcc atagctgttt tgattcattt
       61 aatatcgctt aaacgtattg taaaactttt gtttcttgat atgcgtggtc gatcaaacca
      121 ccacccagac attccattcc atcataaaat acaactgctt gtccaggagt gattgcacgt
      181 acaggttcgt cgaatacgac ttctgcacga ttatcatcca ataagcgaac tgtcacagga
      241 acgtcttgct gacgataacg gaatttcgct gtacacttga attcttttgg cattggttca
      301 ttcgtcgtaa agtggatttc acttgcatcc aagcttgtgg catacaatgc aggatgatgg
      361 aacccttgcc ccacatataa tgtgtttgtc gctagatctt ttcctacaac aaaccagggt
      421 tcttgcgaat ctcctccgcc accgatccct agtccttgac gttgaccaat cgtataatac
      481 atcaagccag catgttgtcc tttgacttca ccatcaagag tgaccatatt tccttttttt
      541 gctggcagat agttgctcaa aaattgtttg aagttctttt caccaataaa gcagattccc
      601 gttgaatctt ttttcttagc agtcgcaagg cctgcgcgtt cagcaattgc ccgtacttct
      661 gatttttcca tgccgcctag agggaacatc gtttttgaaa gctgttcttg tgataattgg
      721 ctaaggaagt aagtttgatc tttattattg tctactccac gaagcatgtg gaccacgccg
      781 ttttcatcgc gagtgacttg cgcataatgt ccagtagcta cataatcggc gccaagatcc
      841 attgcataat ccaagaatgc tttgaatttg atttctttgt tgcacatcac gtctgggtta
      901 ggtgtacgtc ctgcgcggta ttcggctagg aaatattcaa atacacgatc ccaatattct
      961 ttttcaaaat tcacagagta ataaggaatc ccgatttgag cagctacttt cgctacatcc
     1021 ttataatctt ccgtggccgt gcatacaccg ttttcgtccg tgtcatccca atttttcatg
     1081 aatattccga tcacgtcgta tccttgttcc tttaacaaaa gtgctgttac agatgagtcg
     1141 acaccgccgc tcatccctac gacaacgcgt gttttgctgt tgtctgtcat gatatcacca
     1201 tcatttcaat tagattctga aacatctacg agttgaaggt cgcaactttt cagtatttta
     1261 cattatacgt catatttctg aaaatttcat cccttaatta tcaagtcgta aaaatactgt
     1321 tctactggac ctattcagta cacaaacatg atagtcaacc aaaaaatcac tcatcttttg
     1381 agcattcgat ataaaaaatt ggcatactca taaaaagggc cagcctttcg gcaataagga
     1441 ataattttat aaattgtgaa aaactatttt tcaaaattgc ttcttatcgc ttaaagctgc
     1501 acgtctcatt tacaaccttt tctttttaga tgttatgagt tgctcagata agtgattttg
     1561 tttactctaa gaaaaaaggg aggcaaaaga tacatgaatt actttatcag tgatatgcac
     1621 tttttccatg aacgcttatt aggaaaaaac gactttgctc cgcggctctt tgcaaatgtg
     1681 gatgaaatga accaacagtt gatcactagc tggaatgcag taataaaaga aaccgaccat
     1741 gtttatcatc taggcgacct tgctatgcat ccgcaatatg aaaaagcaaa tgcagaaatt
     1801 ttggaactgg tcactcaact gcaaggaaca atccacttga tcaaagggaa ccatgacagt
     1861 cgcgcttttt tccgttattt agaaaaaaat gatcctaggc ttactgacgg aagtcccaaa
     1921 ttttattttt atgatgtagg tgccatcgtt aaattcgacc atcaacaata ttacttgact
     1981 cattatccga tgttgctcgg gcctaacgac actatccgca atcttcatgg acatatccat
     2041 cattacagcg tacctatcga gaacgatgtc aatgtaggcg tagacgcacc ggaaagagaa
     2101 ctcttaaagg aaaaactacc atttggcact ccgttatctg agcaacaaat caaagaaatc
     2161 tatgaagcaa agaggaagta cgataggtcg tgaaaaaact gccctatatc aagtattaaa
     2221 caaataaaga taattactca tatttttcat ttgagtaggc ttattgccac aggaacatta
     2281 ggatcagaaa tggagttgta acaagacttc tgtcacaact ccattccata ctattcgtct
     2341 ttttgctcat ggtgttcttt taccagtttt ttctgtaatt ctttatcata atttcgggca
     2401 gctgacgtta aaaataaaac gattccgcat aataaaatcc cgattccgga aaacaagaac
     2461 attttttcta cccctaaacg atcagctaat ggtcctgcaa ataataatcc aattggacct
     2521 gccaggttca tcaaagagtt gaagttccct aagacacgac ctaaaacatt cgattcataa
     2581 ctctgctgga tcattgccat cagcagtgta ttgaaatatg gcgttgcaca acctgctcct
     2641 gcattcaaga taagaaagta caagaaccct tgactatcgt ttggaaccaa tccgcttgca
     2701 ccgatcgtca ggcccataat gatgtaggct ataataatag gtttcattcg gtctttccat
     2761 gttcctataa aactgatcag tgcaccgcct aagagcattc cgactgcgta gactacttcc
     2821 actgctcccg cctgtccaac ggaaccacca aagtaatcta gcgtcatcaa tggatacaga
     2881 ctgattgctg gcatgaacaa cagcatcaca aatgcaccat tgatcgttat gtaccataac
     2941 cctttattat ccatcaattg ctgcagccca aattttgcat ctttaaaaat agtaagcgga
     3001 acttccaata tttcaggaga aacttttggg atcttcacaa aaatcaataa gccgacacca
     3061 aaaactgccc ccaatacatc taataaaata aggtaattga caggaacgac tgaaaacaaa
     3121 gccgcaccta gagctggcgc aatgatgaaa ttcgctgatt ggaccattcc taactgtcca
     3181 ttcgtccttg ttatgtgaga agatggaacg attgtcggca taatcgactg gatcgttggc
     3241 atttggaatg tttgtgcgac cgaacgcata aacaaagaca caaaaactag ccaaatcggg
     3301 aaggattctg aaatcgttcc gactactgct aaaataagcg cgaaaatcgc tacaataata
     3361 tccgtgacaa tcaaaagagc ttttttatcc cagcgatcca ctagcggtcc tacaaatggg
     3421 cttagtaaaa ccatgggaat cattcctagt aaagtagcaa aactcaaaat tgtcgccgaa
     3481 ccggtttctc ttgtcaaata ccagatgatc gcatattgga cgaccatact agtgattcct
     3541 gacaaaaact gtcccgataa aaagaacaaa taatttctct tccaattcgt taaaacgacc
     3601 gactgttcat tttccataaa catccctctt ttctttttca ttttgtatag cgtttgcaca
     3661 acaatcatta tacgggattc ttttttcaaa aaatac
//
LOCUS       DAZAJZ010000162         3191 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000162 DAZAJZ010000000
VERSION     DAZAJZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.169"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..611)
                     /locus_tag="ACG86D_002884"
     CDS             complement(243..611)
                     /locus_tag="ACG86D_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN3141947.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            761..1243
                     /locus_tag="ACG86D_002885"
     CDS             761..1243
                     /locus_tag="ACG86D_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN3141948.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1280..2290
                     /locus_tag="ACG86D_002886"
     CDS             1280..2290
                     /locus_tag="ACG86D_002886"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN3141949.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2405..3118)
                     /locus_tag="ACG86D_002887"
     CDS             complement(2405..3118)
                     /locus_tag="ACG86D_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141950.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1079 a          515 c          620 g          977 t
ORIGIN      
        1 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagtg
       61 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
      121 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
      181 caaaacctgt ataagctgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
      241 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
      301 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
      361 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
      421 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
      481 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
      541 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tctgattgat gatcggctga
      601 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
      661 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
      721 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
      781 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
      841 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
      901 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
      961 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     1021 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     1081 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     1141 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     1201 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     1261 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     1321 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     1381 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     1441 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     1501 tgaaaatatc caaatgatct ttccgccttg gggaggagaa ttattgattg agattctcga
     1561 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     1621 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ccaaccttat
     1681 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     1741 aagagaagga gaatcgattc tacagcaacc ttctgaaaag tatcaaaaag agtggcccca
     1801 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     1861 cggagatgca gtaaggatga agggacgatt attaggtggc tgtattgata cgatcagtca
     1921 tttaatcggt accccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1981 gccgatcttg tggtttctag aaaattgtga gctatcgtca gttgatttaa gaagatcact
     2041 tgttcagatg aaactggcag gatggtttga tggcagctcg gggatcatgt tcggtagaag
     2101 tgcagcagag gatttgttag attataaaat gaaggatgta tatgaagatc tgtcaaagga
     2161 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     2221 gatcaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     2281 gtttatttaa aaaatttttt cctgccttgg agtttatcaa agaccaacaa atctaaatag
     2341 aaaagaaaat gaaagaagct gacagtattt tgccagcttc tttttgtatg atctattaga
     2401 aattttaatt atccataact atcacattga tcagctgttt cacatcattt ggattacctg
     2461 taggaatagc tactacagat gcattatcaa taatttcttg agttgtttct ctaatgaacg
     2521 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     2581 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     2641 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     2701 ctgcaggata atgaataaca tgtagttcga aatcacccga tgtatctcta aaagtattac
     2761 ctctaattgc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     2821 gatctattga ctgagaagac tgtgaggaag ttgtgtctat tgttgaagaa gaaatatttg
     2881 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     2941 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     3001 tttgtttgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     3061 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     3121 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     3181 aaaccttgtc t
//
LOCUS       DAZAJZ010000163         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000163 DAZAJZ010000000
VERSION     DAZAJZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.170"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACG86D_002888"
     CDS             complement(63..2555)
                     /locus_tag="ACG86D_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN3141951.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACG86D_002889"
     CDS             complement(2562..3029)
                     /locus_tag="ACG86D_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN3141952.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZAJZ010000164         3108 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000164 DAZAJZ010000000
VERSION     DAZAJZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.171"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1338)
                     /locus_tag="ACG86D_002890"
     CDS             complement(<1..1338)
                     /locus_tag="ACG86D_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3141953.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIER"
     gene            complement(1402..1761)
                     /locus_tag="ACG86D_002891"
                     /pseudo
     CDS             complement(1402..1761)
                     /locus_tag="ACG86D_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(1939..2259)
                     /locus_tag="ACG86D_002892"
                     /pseudo
     CDS             complement(1939..2259)
                     /locus_tag="ACG86D_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2375..>3108)
                     /locus_tag="ACG86D_002893"
     CDS             complement(2375..>3108)
                     /locus_tag="ACG86D_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141954.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT          962 a          576 c          416 g         1154 t
ORIGIN      
        1 tctctctatt tccttaacat actgtctaaa tcgaatctct tgataggtgg gatatgttaa
       61 agctgaatta ttttggatat caacattctt ttgtctaaaa tatctatcat atttctcaaa
      121 aagctgctgc ttatcagtgt ctttcttcca cgtttgataa agatactcta taagttcgtt
      181 gatattaaaa acttcgtcta ctttttccaa atcatccatt tgaatatatt ttaattcatt
      241 ctgtcttttc ataaccttca tgccgttgaa attcacccat tgtggtaaat gatacatctg
      301 aatataaagt tcttccaaat catctaagaa ataaaaggca ttggcacagg tcaattgaat
      361 gatatttttc aaattgccaa cattttcact aaagcgatag tttaataaaa aatctaataa
      421 ttgttgagat atcagtattt tctttgctag tctttcttct tctgatttaa aaaatatctt
      481 gattaactgt tttctttcat aaagagaacg actgtctaat gttggaagtg ttagctttac
      541 aggtattctt ctcaacaaag ttgataatag tttatcctca ggtggttctg atgttgcaaa
      601 tataatccag caacttgatg tgtaccagtt ttcattatca cccatccgat gaaatttcct
      661 cgtatccata aactgaaaaa gtttctcctg acattcattg ctcaatgcat gaatttcatc
      721 taaaaataag actccattat tagctaattc tgctaaaccc tttcttccat cttcggctcc
      781 tgtatatgca ccttttacat gcccaaaaaa attggacaag aaaagttctg gattattggc
      841 atattcacta caattaactg aaatgaaaga tgccttctct tctatataat tattggccaa
      901 caaatcctta tacacataat ttgctaaata gctttttcca gttccagtct cgccatgaag
      961 taaaatgggt aagccatttg gcggatagta gattgctgct ttaatttgag caattacatt
     1021 tcgcagactt ccttgacttc cgataaccct ttctattcca accctaaaag agctatcata
     1081 ccatttttcc gcttcctctg aagagctaaa aatttgttga tttggaactt tctccggtaa
     1141 agaaaagaag attactggac gagtattgat ttttccaaat tctccatctt ttacgaattc
     1201 attcaaatat tgagagacaa tattacgact aacatttaat ttttcagata ttttctgtgc
     1261 tgtattgttc ccccatttat ttttaatctc agagttctct atgacataat tgacaatgcg
     1321 ttcttttaat tcttccatca cagcacctcc tcgacactat tcgaattttg ccgacagtat
     1381 ttttccataa atatgacttg ctaaaaaggc tttgtttaga tcattcaatt ggaacgttgc
     1441 ttgttttgct tgatccgcta agacgttgaa aagatactct ggcgaaatcg cataccccaa
     1501 aatatcaacg attgttgcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
     1561 atacaacatc gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
     1621 aaccacttgt tccaacaaac ggtcggataa atatttatag aaaatcagcc ccaataaata
     1681 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataactt
     1741 ctgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
     1801 ccctcttatt ttagcatact cagttttaga aaaagcaaaa aaataaagaa acttattctt
     1861 gataacaagc tacgtgacgt atatccctaa cacaaaaata gccatatcct ttggtttgga
     1921 tacggctatt tgatattact cattcaatta tcaaatcaca gtaggtgtga atctcatcgg
     1981 gatcggcaat atctacttct gattaggatc aaaggtgatt ttctcaataa aggtgtgata
     2041 caaagcttta acttgcttct tctcactctg gataagaaag caatccaaac tagtcaatat
     2101 ctgttggaca tctttgactt gaatcggttc cccttgttgt tgaaagatcc ctaagatttc
     2161 attctctcga atttgtaact gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa
     2221 catcggaaac aattcgggac tatcttccaa agcagcttcc tgtttttgct ctaatagtta
     2281 gattttgcgt accattttct tcttttattt tccttcgatt gctgatttta atatagtcta
     2341 aaggaaattt atatttattg tattctttat aacttcaaga gaacttcgtt tgagtattac
     2401 tgtatttttt taattccata agaaaattag ttattacctg ccacctaatc agcaaatctg
     2461 aacctgagtc aatataatta atattatttt ccatctccaa cattgtaagt actgttggtg
     2521 ttaatatagg gactttgtac aattgtattt ttttgatttt atcatctgca caaaggagat
     2581 tttttaaatc taatccttct tttatttcaa aagttgaatt ttttaaatct ataagagtaa
     2641 aaccaattcc ttcatactga atttctgaaa atcgaatttt tctcccagtt gatagatcaa
     2701 ggggcagaaa accttttatt acattgtgag tgtcaccatc atttacaatg tcattaggaa
     2761 ttttgatttt agaattctgt ggagcaccta ttggaagata ataattcatt ataattttgt
     2821 agtcttcatg aggaaataaa gtacttttag gattgctaag atattctcgt accgaatcta
     2881 agaatggctt atccattgaa tcttcatccc catttaaact aaaaaacatt cctacaattg
     2941 cttttgcaac tctaagtgaa ttcattttct ttattgaatg tgttaaatca gacttaccat
     3001 tctttaaact tgtattgtta taaaggaaat ttttattgtc ttctgcaaat ctaatatatt
     3061 cctcaacata ctcactacca aaaaaactat tacatttact acataacg
//
LOCUS       DAZAJZ010000165         2942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000165 DAZAJZ010000000
VERSION     DAZAJZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.172"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            616..>2942
                     /gene="esp"
                     /locus_tag="ACG86D_002894"
     CDS             616..>2942
                     /gene="esp"
                     /locus_tag="ACG86D_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN3141955.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1071 a          447 c          573 g          851 t
ORIGIN      
        1 tattcatgta gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat
       61 caataattta atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa
      121 aatgttgata actccaatca ttcgtttaag attctaggaa tcattcagaa atgctgtgaa
      181 accgatactt tataattata aagaaggtga tttcaaattc cgaagaagga atttgaagtc
      241 acctttttac ttatgtcaca gtctcattca gtaatgcggg ataaggctcc ttattccgtt
      301 atttgaatac tctgaagttt aatctaagaa agggtaatat tttgaaaaca tgcttcatcg
      361 catagaaatg aacttaattt cagaaagaat acatacatat ctttttatat gttttcagct
      421 gctgaatggt taaaaagcac aaatatccga aaaaaacatg aagaaatgta ctttttttgt
      481 tgtaatgttc atgaaattct ctaagtaaat tagttaacat aaggttagta agattcctat
      541 tctgtgaata atacgtatca gatttatctt ttattcttac taggaaagta attattaagg
      601 gagttgattt gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag
      661 aaacgagtat tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt
      721 ggtgttggtc ttgtcttggg aacaactgaa ctagttaaag cacaagatga aatttctcca
      781 agcagcccac ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg
      841 aatacttttc aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt
      901 agtcctcaag aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac
      961 ggaaaacata atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa
     1021 cgattagcta aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct
     1081 agacggacct ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc
     1141 aattatattc gtgcaagtga tggattattt gctggggcag aaacaaccga tacgcaaacc
     1201 gctcctaatg gggtaattac gtttgaaaag agcctagatg aaatttttaa agagataggc
     1261 atagacaatc tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac
     1321 gatgataaaa ttgtaccggg tattgatagt acgggctact ttttaacaga ttcagatgat
     1381 ttctataata gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta
     1441 agtgcaaaat atgagaaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct
     1501 aatggagcta taatcttaga tcataagtta acgaaaaatt ataatttctc atattcatct
     1561 tccgcaaagg gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat
     1621 gtagcgggta tacagatgca catggttcag gctgacaaag taacatatga tgtttcgttt
     1681 gaatccggaa caaaagtagc tgatttagca atcgagcgac gggaaggtca tgaaaattat
     1741 ggcataggtt caataactaa taatgattta actaaactta tcgattttgc taatgcaagt
     1801 ccacgtccag tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta
     1861 gaagatatga aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc
     1921 tttgattctt ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt
     1981 tattattatt tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga
     2041 gacacgaatc catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca
     2101 gttaagggga aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt
     2161 gataaagaag gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct
     2221 ccaatttcag atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg
     2281 aaagatgaat taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca
     2341 accgttatta aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa
     2401 ccagatgcta aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca
     2461 tggaaaacag agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt
     2521 aagatcggaa atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct
     2581 atggaaaatg atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt
     2641 ggaaaaccta catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttca
     2701 gatgtctata ataagtactt cggtaatgca agtgttgatg caaacactgg tattgttaca
     2761 ttcactccag ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa
     2821 attgtttacc aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat
     2881 atttatgaaa atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca
     2941 gg
//
LOCUS       DAZAJZ010000166         2675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000166 DAZAJZ010000000
VERSION     DAZAJZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.173"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACG86D_002895"
     CDS             699..785
                     /locus_tag="ACG86D_002895"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN3141956.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACG86D_002896"
     CDS             complement(1087..1311)
                     /locus_tag="ACG86D_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141957.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACG86D_002897"
     CDS             1677..1961
                     /locus_tag="ACG86D_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141958.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACG86D_002898"
     CDS             1964..2380
                     /locus_tag="ACG86D_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141959.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACG86D_002899"
     CDS             complement(2517..>2675)
                     /locus_tag="ACG86D_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3141960.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAZAJZ010000167         2636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000167 DAZAJZ010000000
VERSION     DAZAJZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.174"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..2568
                     /locus_tag="ACG86D_002900"
     CDS             127..2568
                     /locus_tag="ACG86D_002900"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN3141961.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          890 a          461 c          617 g          668 t
ORIGIN      
        1 taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct tagaaaaaaa
       61 taactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt gtaggaggtg
      121 gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg aaagtatcgc
      181 ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa aggattatta
      241 gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tatttggaaa tgtcctgcaa
      301 gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct ccctgtagat
      361 gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt gatcttagca
      421 cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac agagagtatg
      481 tctcaagcac ctatgctgaa acagtatcag tcagaaacaa atgaatatgg tgaaccaatt
      541 tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat gggattaacc
      601 gcagagaatg ttgcaacaca attttctgtg agcagagaag agcaggatcg ctatgccttg
      661 tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga ggagatcatc
      721 ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg tggaaatagt
      781 acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac tgtaacagca
      841 ggaaatgctt ccccgttgaa tgacggtgcc tctgtggtga tccttgcatc caaagaatac
      901 gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga ggtcggtatt
      961 gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac agatagatca
     1021 ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc agcttcttct
     1081 atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata tggaggagca
     1141 atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac attagcttac
     1201 ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg tggaggttta
     1261 ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt gcaaaaaaaa
     1321 aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa aaatgtattg
     1381 acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct ctccgatcac
     1441 atgatcgaaa accaagtgag tgaagtggaa atcccaatgg ggatcgcgca gaattttcaa
     1501 atcaatggca agaaaaaatg gattccgatg gcaacagagg aaccatctgt cattgcagct
     1561 gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca gcggctgatg
     1621 cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa tgcagttaat
     1681 catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat cgtgaaacgc
     1741 gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca tgcatactta
     1801 tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat caactcgatt
     1861 cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat tctattcagt
     1921 attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat cccattcgaa
     1981 cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca acaggcaggg
     2041 gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat catgaatggt
     2101 atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc ttcgatccac
     2161 gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat caaaggggat
     2221 aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg agcttccaat
     2281 atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc caaggaacta
     2341 gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg tgcattagtt
     2401 acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc gatctcaatt
     2461 ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc agaaaagatg
     2521 aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa aagcaatatc
     2581 tttagaagaa taaagcaaga tttacagtta ttttatttac tgcagatctt gctttt
//
LOCUS       DAZAJZ010000168         2633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000168 DAZAJZ010000000
VERSION     DAZAJZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.175"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACG86D_002901"
     CDS             120..626
                     /locus_tag="ACG86D_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN3141962.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACG86D_002902"
     CDS             643..1542
                     /locus_tag="ACG86D_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN3141963.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACG86D_002903"
     CDS             1629..2513
                     /locus_tag="ACG86D_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN3141964.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          958 a          489 c          515 g          671 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAZAJZ010000169         2505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000169 DAZAJZ010000000
VERSION     DAZAJZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.176"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1149
                     /locus_tag="ACG86D_002904"
     CDS             211..1149
                     /locus_tag="ACG86D_002904"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFN3141965.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1401..2411
                     /locus_tag="ACG86D_002905"
     CDS             1401..2411
                     /locus_tag="ACG86D_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFN3141966.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
BASE COUNT          910 a          316 c          462 g          817 t
ORIGIN      
        1 agtaattata agagaacaaa agtaaagaat tatatctact tgcttgcgta tttctttttt
       61 atcattgtgt gtttgatatt catcaatata agaggaactg cttttgtaaa tggaatccta
      121 ccattattta gagaaaatat tatttttaag gctgtttact atttttttga gtaagaataa
      181 atagcactta aaaatagaga tgagggattt atgggaagac aagaattata tagtttagct
      241 atcaatgaat tggaaaaaag aaatcttgaa atacatgaat taaatcaaaa aaataattct
      301 attttaggca tgaaaatacg aaataaaatt aatcaactta accaaatgaa aaaacgagat
      361 aatctgttgc cagcaaaata tacccttaaa tcgatgaccg gtaaggggcc tattaaagac
      421 tattcaaaca gagagaatcg cagtgatgaa ctaagaaaaa aagagttaac taatcaagga
      481 acacctaaaa ggattgctgt gtatagttgt atcactggta attatgattc tattaaagaa
      541 ccggattttt ctcatccatt ggtagattac tatttgtata cagataatgt gattacaagt
      601 gagcattgga atattagaga gatacctaaa tatatatcgg aattaaaaaa taatattttg
      661 atcaatagat atattaaatt tcatcctaat gagctattta ctgactacga ttttactttg
      721 tatgttgatg gtaatgtaac aataacttct gatattaccg gattactgaa cagttcttcc
      781 aaacttgggt taagtatgca taaacatagt gaaagaaaat ctttatattc tgaggcagat
      841 gcattaattg cagttggcaa aggtaataag gaaagtatta tagagcaaat tagtaagtac
      901 agagaagaag gatttcctga taattttgga ttggctgaag ccacggttat tttagttgat
      961 ttaaataatt caatcgctga gaatcttcta gatttgtggt ggagagagta tttgaactca
     1021 gaaagtgggc gagatcagct cgcatttcct tacgtgttgt ggaagtccgg acatgcaatg
     1081 aaggaagtga ctaccttagg caataatgta tataaaagcg ctaaattaag aatcataagt
     1141 cattattagc cctaaatgat ttctaaacta ttctttcaaa tcctagtaaa tatttggaga
     1201 tagaagattt cctttttaaa gtcataaaaa attaagttat attttttgag tgaatggaga
     1261 agacgcatag tatcataaac ttcgttatat cttaaattat ggggtatgcc taaataataa
     1321 atgtaatacg agtataaaga agaaagcaat tttgggttta accctattgg ttcctttcag
     1381 aaaaaggtgg atgaataaaa atgaaaatat taataacagg tggagcagga tttattggtt
     1441 ttcatttgtc tagaaaatta ctcaaggaaa ctgaaaatca aattgtagcg attgataatc
     1501 tcaatgatta ttatgatgtc caattaaaag aagatcgatt aacgatactg tacgattata
     1561 ataattttaa atttatcaga atggatctat tagagaaaga aaaattagat tctctatttg
     1621 aaagtgaagg ttttgacata gtaattcatt tagctgctca agcaggtgtt agatattcta
     1681 ttgaaaaccc ttatgcttat attgattcta atattatcgg ttttcttaat attcttgaag
     1741 cctgccgaaa cttccctgta aagcatctat tatatgcatc atctagttca gtatatggtg
     1801 ggaacacgaa agtaccgttt tcaacagaag attcggtgga tcatccagtt agcctttatg
     1861 cggctaccaa aaaatcgaat gaattaatgg ctcatactta tagccacctt tataaaatac
     1921 caacaactgg gctgagattt ttcactgtct atggtccatt tgggcgacca gatatggcct
     1981 atttctcttt taccaagaat attttatctg gaaaagaaat taagattttt aaccatggaa
     2041 agatggaaag agatttcact tacatcgatg atatcgtaga gggaatcact agattaatca
     2101 ataaagtacc tgaacccaac tccgactgga cagacatcga tgatttgagt agtgaaagtt
     2161 gggcaccata taagatatac aatattggaa ataataagcc ggttgcattg ttggagtttg
     2221 ttgaaacaat tgaaaagtat ttaggtgtta aagcgaacaa gatttttttg gacatgcagc
     2281 cgggtgatgt cgttagaact tatgccgata ttgaagattt agaggaaaaa atcaacttta
     2341 aaccgaatat aaccttggat gagggcatta aaaaattcgt tgaatggtat acagtgtatt
     2401 atttgagtta attttgaaag gaatattgaa gatgggtaat tcatacgatt tttttagctt
     2461 tgttaggtat tttaggaaat tctactttca aacgtgacga acttt
//
LOCUS       DAZAJZ010000170         2441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000170 DAZAJZ010000000
VERSION     DAZAJZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.177"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..2291
                     /locus_tag="ACG86D_002906"
     CDS             138..2291
                     /locus_tag="ACG86D_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN3141967.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          833 a          427 c          541 g          640 t
ORIGIN      
        1 gtgattgctg tgaaacaaaa agttgatttt atcttgaaat taggtaaaga atttcttgct
       61 ccgcccattt tttctttttt attaagaagt ttttgggtac aatgaagcat gaatgtaaat
      121 aggcggaagg ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt
      181 tagaaaaaat aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg
      241 ttccttttcg cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg
      301 ctagattggg ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta
      361 cttctacgat tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa
      421 aagctttagt tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta
      481 cggctaaaga tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag
      541 atgaaaatct gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga
      601 aagaagccac aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata
      661 gtgcgatggt caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa
      721 ttgctaccat ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa
      781 actctggtgt tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag
      841 gagtttcgac tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta
      901 ttcttggcag tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc
      961 tagcaaaagg ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg
     1021 aagatgtcct gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg
     1081 tttcgaatca aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga
     1141 atgcagaatt ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca
     1201 acaacggcaa agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag
     1261 gagaagtgct agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga
     1321 atgctttagg cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt
     1381 tgacagcagg ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga
     1441 ttcgtctgca aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc
     1501 ttgatgcagt caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa
     1561 agatgttagg actagactat acacctggaa tgggactacc tagtttagat gaagaagcta
     1621 aagcttatca gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga
     1681 tcgatttgcc gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca
     1741 atgcggataa tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac
     1801 agtttgacac atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg
     1861 ggaaacgagt acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact
     1921 tgggtgaagt gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag
     1981 aaaatatggc gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca
     2041 caacagccaa accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag
     2101 aaacggttgc ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg
     2161 gaccaacaga caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac
     2221 aagaccacat aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca
     2281 tggcaaaata aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt
     2341 tgtgaaaata gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt
     2401 tgaacaccgt ttactcggca aaaaaggttg tgacaaaaat t
//
LOCUS       DAZAJZ010000171         2399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000171 DAZAJZ010000000
VERSION     DAZAJZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.178"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG86D_002907"
     CDS             207..2069
                     /locus_tag="ACG86D_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3141968.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="ACG86D_002908"
     CDS             2069..>2399
                     /locus_tag="ACG86D_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3141969.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DAZAJZ010000172         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000172 DAZAJZ010000000
VERSION     DAZAJZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.179"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG86D_002909"
     CDS             316..570
                     /locus_tag="ACG86D_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3141970.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG86D_002910"
     CDS             571..888
                     /locus_tag="ACG86D_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN3141971.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG86D_002911"
     CDS             1447..2382
                     /locus_tag="ACG86D_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141972.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZAJZ010000173         2237 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000173 DAZAJZ010000000
VERSION     DAZAJZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.181"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1687
                     /locus_tag="ACG86D_002912"
     CDS             74..1687
                     /locus_tag="ACG86D_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3141973.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          697 a          398 c          444 g          698 t
ORIGIN      
        1 aattttccgg cacaattatt tgctgttttt taccataaca tagtacgatc aacgtagaaa
       61 aggagatgtt tttatgaaag ttgttgtcat tggcggtgta gctggcggtc cttcatttgc
      121 cactcgtttc cgtcgattga atgaagcgca cgaaatcatt atctatgaac gcggagaaaa
      181 tatttcttat gcaagttgtg ctttgcctta ttatttagga ggtgtgatca ctgaccgtga
      241 ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg atgtctttac
      301 taaacacgaa gtaacagcaa tcgatccttc tactaagcga ttaacagtta aagacctatc
      361 cacaaatgaa gaaataaaaa cagattacga taagttgatc atctcttctg gtgctagacc
      421 agattatccg gatattcccg gagtttttga agcagaaaac ggctttgtcc tccgtagtgt
      481 gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa aatccacaac atgtcgtcat
      541 tcttggtgca ggtgttatgg gtctggaatt agctgagaat ttcaagcatc gcggcttaga
      601 cgtgacttta atcgatcaat tgccacaagt cgctttccct tatgatccag aaattgctaa
      661 tttagtttat gacaaattgc tgaaagaagg attagccgtt catttagaaa caagagttac
      721 tgagatccgt gataaaggtc gagaaatagt attatcagat ggctccgtcc tttctgctga
      781 tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag caaccggcat
      841 acaattatca gatacaggtc agatcattgt tgatgaccag ttacaaacca atctgccgga
      901 catctatgcg attggcgata ttatcgaaac gactagtgta gtgactggtc agccgatcca
      961 aagtatgctt tccagtgcgg ccaatcgtca aggacatatg ctggcagata ttttaaatgg
     1021 tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct ttgatcatac
     1081 agcaagttat gcgggaatga cagaacatga attaaaagca tcaggcataa caaattataa
     1141 aactgttttt atcactccct ttgaccatgc ctatttctat ccagaagcta caagattaaa
     1201 tctaaagctg atttttgatg cagatagcgg tcgtatttta ggtggacaag catttggaga
     1261 aaaaggtgtc gataaacgga tgggagaact ttctgtagca atcaccggaa acttgacagt
     1321 ctttgatttg cctgatttgg agttgcctta ctctccaccg tattctacta cccgtgaccc
     1381 gttgaatata gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag aaacgatcaa
     1441 agcaagtgat atacccgaaa acgatttgaa agaagcgttc tttttagata tacgtgaacc
     1501 taataaagca ccttctggca gtatctcggc aacaaaaaat attccaatga atgagttacg
     1561 agatcgaatc aatgaaatcc caaaagataa aaaagtttac attactttca gaagaggatt
     1621 gaatacttat acttctgccc gaatcttggc aggtttgggt atcaaagcga ttttgattga
     1681 agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg agttattttt acttgaggag
     1741 taaaaatgaa aaagtatata gggttgttgg ttggtatgta tatagaatag aagataaatg
     1801 tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga aagtttaaac
     1861 attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt gtgactctga
     1921 gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt acgaggcatc
     1981 atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt ttttcttata
     2041 taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc gttgtgtgat
     2101 actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt cgccatcctc
     2161 gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa tttaattttt
     2221 ttgttcctat ttattta
//
LOCUS       DAZAJZ010000174         2109 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000174 DAZAJZ010000000
VERSION     DAZAJZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.182"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG86D_002913"
     CDS             complement(<1..426)
                     /locus_tag="ACG86D_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN3141974.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACG86D_002914"
     CDS             complement(535..1566)
                     /locus_tag="ACG86D_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3141975.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          658 a          338 c          361 g          752 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaaa ccatcacaac gagtggaaat tatatgaaat ttttcataaa
     2101 aattcatat
//
LOCUS       DAZAJZ010000175         2058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000175 DAZAJZ010000000
VERSION     DAZAJZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.183"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..1694
                     /locus_tag="ACG86D_002915"
                     /pseudo
     CDS             59..1694
                     /locus_tag="ACG86D_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          679 a          310 c          364 g          705 t
ORIGIN      
        1 tttaaaattt aagttatact agataaagca atccatgaaa ggagtatata attaagtaat
       61 gcaagtaaag aaaaaaataa tattgtccat tctcctagca attattgctg gactttattc
      121 aatcaattat tttagaaatg ttcatactat ttctacatcg ggggatattg ccttccattt
      181 tgctagagtc aaagggttat cttcaatttt ttctggtcca atcaatttta cgacgtttaa
      241 tcattatggg agcggagtga attattttta tccctacttg accttttttc cggcagtcat
      301 tttttattgg ataagtaata atttaatcgt tagttatatc ttatatgttt ggctattgaa
      361 tgtttgtact atcatgctga tgtttcatta tggactaaaa tttttaaaaa gaatcgatgc
      421 ggcttttata tttagctgtt tatatacatt ttatgggtac cgtacgattg atatttatca
      481 tcgttctgct attgctgaag cgattgcact tacggttata ccgatagtta tgtattatgc
      541 ttacgcactg atttatgaga aaaagccatg tgcaattcca ttagcagtca gtatgagctt
      601 actcatttat acacatgttc tttccacttt tatgagtgta atagccgttg gcttactagt
      661 cttaggcggc tttgtagtaa gtgaaacaag aaaaaaagaa agcatccggt tgattgtgga
      721 gttgtcaaag gcagtaggaa tgacgattct tttaacagca tacttttggt atccgatgtt
      781 ccgacaaatc atggttcaat cgattaatga gccagataaa acgaccttac aacaccaagc
      841 attaaatgtc tcggatagtt tgattggtgc gatgaataat gacctgacaa cctttacggt
      901 aggaatcatt ggtattattt ctttgatctg tccactcttc ttttttaaag gatttaatcg
      961 caaagaaaaa gtgatttacc ttagtgcggt agtctcttgg ctcttatcca ccaaattttt
     1021 tccatggtct ttattacaaa atacacccat tcaaatgatt caatttcctt ggcgaatctt
     1081 aggctttcaa gtactgtttg gctcgttgat tttatcgttc gttttcatga agagcacatc
     1141 agctaagcaa agaaaagtag ttaatataac ggcaattgtt ttattactgt tggctgtatc
     1201 tgctgcaaca aaagcaaact atgaacataa agttgtcact cagagagatc acttggtaat
     1261 gaatcaagaa aatcttattc gttacacgac actccttccc ggaggacttt atgactatgc
     1321 acccacggaa gcattacaat acaaagaaca tttgcagaat catgaagtaa gaattggcaa
     1381 ggaatggcat actatgcctt atcaagtgat ggattcgaaa atagtctata cagtacatga
     1441 atctaaagga caaaaaataa cgcttcctgt atttgattat tggggaacag tcgcaaaagt
     1501 aaatggacaa aaaagagaga ttgataatag gactagtgtc gattgaagga acacaaggag
     1561 aaacaatcat tgaagtttct tccatttatc ctccaactat gaagttagct tttttaatta
     1621 gtttatgtag ttacctcttt cttttaatta gttttcttat caggaagtta caacatataa
     1681 aaataatgat ctgattacat ttttttcaat attagcttac tagagaataa gaaaaagtaa
     1741 gtaataaacc aaatttaaac tttatattat aataatgaag cttaatcttc tttatagaga
     1801 attgggctat ttttaatggc aaagagggga agaagatgtt tcaaaacaaa ggacaacgct
     1861 atgttgtaaa aggagtaaag gaatcttact ggaatcaaaa atctttattg agaaaattat
     1921 ctttgaattt aagcacaaag aaatgaaggt acactagata gcaatgaaga tatctttgtc
     1981 attaaacgtc gctattttac acaagtaagt gatgatttga ttcatgtctt ttccaatgag
     2041 tggtttgttt ctgagaaa
//
LOCUS       DAZAJZ010000176         1928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000176 DAZAJZ010000000
VERSION     DAZAJZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.184"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACG86D_002916"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACG86D_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN3141976.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACG86D_002917"
     CDS             853..1041
                     /locus_tag="ACG86D_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN3141977.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACG86D_002918"
     CDS             1054..1611
                     /locus_tag="ACG86D_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3141978.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACG86D_002919"
     CDS             1643..1885
                     /locus_tag="ACG86D_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3141979.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          710 a          296 c          396 g          526 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttg
//
LOCUS       DAZAJZ010000177         1925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000177 DAZAJZ010000000
VERSION     DAZAJZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.185"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..170
                     /locus_tag="ACG86D_002920"
     CDS             15..170
                     /locus_tag="ACG86D_002920"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3141980.1"
                     /translation="MSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSI
                     ENVDFSK"
     gene            184..933
                     /locus_tag="ACG86D_002921"
     CDS             184..933
                     /locus_tag="ACG86D_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3141981.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            933..1763
                     /locus_tag="ACG86D_002922"
     CDS             933..1763
                     /locus_tag="ACG86D_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3141982.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT          603 a          258 c          384 g          680 t
ORIGIN      
        1 ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca atatggaaag gcagtctttt
       61 taactcctga agaattagaa gccacaaaaa aaattcttga tcaagaagtg aaaatatttg
      121 tgcaacaagt cccatcttca agtattgaaa atgtagattt tagtaagtaa ggggggaaaa
      181 taaatgttat taaaagccat tttattaggt ttagttggtg tggtagctgt tattgactcc
      241 aggttaattg gtcgacaaaa tattggtaga ccactaattt tgagtacact tgccggactt
      301 gtgctaggtg acttgcatac agggattgtt cttggggcat ctttagaatt aatttctatg
      361 ggattcgtct caattggggc ggctggacct cctaatatgc aacttggtag tattattgcc
      421 actgcttttg caattttaac taatagtagt acagaaacag ccttaacaat agctatccca
      481 gtggctgtag ctggtgaatt tatgagtatt atcatgcgca tgataattgc tcaatttgga
      541 catgctgcag ataaagccat tgaaaataat aaatttagaa aagctcaaat tattcatatt
      601 tattggtcat ttatttttaa tgcttttgtt tactttattc caatcttttt aacagtatat
      661 tttggtgctg acgttgtggc agatttagta gataaaattc cagaaatgat tacagacgcc
      721 ttgactgtct ctggaaatat gctttccgca ttagggtttg caatgctatt aagcacaatg
      781 ttgagtaaaa aattatatcc ttactttatt tttggattct ttattgttgc ctattcaggt
      841 ctttcactaa ttggcgtgac aatttttgca gctctgattg cctttgtaat ggatcaagtt
      901 aaatatggaa aaagagaaga ggcgcatggt taatggaaaa tagtacagaa aaaaaacgtt
      961 taatcacaaa aaaagatctt aataagatgt gtcttcgttc atggacaatc atgtgttcgt
     1021 ggaattatga aagacaaatg catatgggat ttatgtatgg gatggcgcca attttaaata
     1081 aagtttataa aaatgatgaa gagaaaaaaa gagaagctta tactcgtcat atggcatttt
     1141 ttaactgtac gcctcaaatg acttcattta ttatgggact ttgtgcttct atggaagaac
     1201 aaaatgctca agcagaagaa ggaaacttta atactgaatc tatctccatg attaaaacca
     1261 gtttaatggg tccttttgct ggaattggtg attcattttt ccaaggtact ttaagaatta
     1321 ttacatttgg gattggattg tcttttgcta agcaaggtag tattttagga ccgattttag
     1381 ctgttggcct ttttgctata ccttctattt tagctgtttt ttacggcggt aaattaggat
     1441 atgagtctgg caataaatac cttactaaat tgtatcaaga aggtcttatg gatcgagtaa
     1501 tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg aatggtggct agcatggtgc
     1561 caattgtcac ccctttaaaa ttcactacag ggggaacaga acttgtttta caagacttac
     1621 ttgattccat tattcctgga ttattaccac ttgtatgtac tttgggaata tataaattga
     1681 ttaagaaaaa tgttagtaca aatatgctct tattaggttg tattttggca gggtttgttt
     1741 taagtataat aggtatctta taatgattaa ggctctaatt tttgatatgg acggagtttt
     1801 aattgatagc gagattatgt atcaaaagtg ggtatttaat tttttaaaac aagaaaattt
     1861 aagctatcct ttgtattctt atcaaaaaaa gattggaact acagcaagta tttttgatga
     1921 tatag
//
LOCUS       DAZAJZ010000178         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000178 DAZAJZ010000000
VERSION     DAZAJZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.186"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACG86D_002923"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACG86D_002923"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN3141983.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAZAJZ010000179         1850 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000179 DAZAJZ010000000
VERSION     DAZAJZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.187"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG86D_002924"
     CDS             288..695
                     /locus_tag="ACG86D_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141984.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG86D_002925"
     CDS             709..1110
                     /locus_tag="ACG86D_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141985.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACG86D_002926"
     CDS             1112..1483
                     /locus_tag="ACG86D_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141986.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="ACG86D_002927"
     CDS             complement(1519..1818)
                     /locus_tag="ACG86D_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3141987.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          689 a          243 c          311 g          607 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
     1801 tgtctcatta aaaagcataa ccattactcc gatctgtttt taaaattagt
//
LOCUS       DAZAJZ010000180         1839 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000180 DAZAJZ010000000
VERSION     DAZAJZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.188"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..239)
                     /locus_tag="ACG86D_002928"
     CDS             complement(<1..239)
                     /locus_tag="ACG86D_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3141988.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMIL"
     gene            complement(289..750)
                     /locus_tag="ACG86D_002929"
     CDS             complement(289..750)
                     /locus_tag="ACG86D_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3141989.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            complement(732..1706)
                     /locus_tag="ACG86D_002930"
     CDS             complement(732..1706)
                     /locus_tag="ACG86D_002930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3141990.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          603 a          338 c          234 g          664 t
ORIGIN      
        1 attaaaatca tgtaattatc atttggggat gtttccataa tccctttaac atcactaatt
       61 ttaacaatgt ctaatttgca atcagcaggt ttagcaattg tcaaagccat cacagacatt
      121 ggatctcctg gaactttgtt atcagccaca ataatatggt taatatcata ctgtttagtc
      181 caagaaaata ttacttgtcc atgaagtaat ctgtggtcta cacgcactaa tttaatcatt
      241 ctatttcctc cttcaatttt taaaatttat agaatagaaa ttgccacctt aactgacatc
      301 ttttaaaagc gaattaatat gaaccatact tttttgagag ttattcagta attccttaaa
      361 atctatttct tctatagatt taccagctga ctgggccatt aataactcca gtaaaagagg
      421 taaattaatc ccagcaataa tttgaattct attgttgttc aactgtttaa tagaaacatt
      481 attaggtgtt cctccaatta aatccgttaa aataattaga ggttgcaaat gatgctgact
      541 aattatttct ttcatactct cctcaaaatc atctggacta gtattttctt taaatggaac
      601 agcactaata cctgtggcgt cattcattaa aattaatttt gttgccttta aataaccctc
      661 acaaagggta gaatgtgttg taatcacaat gtgaaataat tgatttgcca tcttttgcaa
      721 cctcctatta attaatattt catttgccac atatagcgtc taacatctaa ggaatggcct
      781 ctttcaaaag caatcgcttc taccatttgg cgaataacaa cgccactaag tacagctgca
      841 atatattctt tcgctacact atctatagat tctaagtcaa aatcagcttg gtctaaaacc
      901 attaatttat tactgaattt tttagtgaag tcttctaccc gttgatctaa aaatcttgta
      961 tctccaatac ttttcactaa gatgaatggt acatcataat cagtgatttc aaaaggacca
     1021 tggaaaaatt ccccagaatg aatagcatta gaattaatcc attgcatttc catgaaccag
     1081 caagctgctg tggagtaaat ttctccgtaa ttgataccgc taccaattgt gtaaataatt
     1141 ttatctcgtt tttggttttt accccattct ttagcagtta tttcaaattt gctcttttca
     1201 gcaataacaa gatcatttaa cttatcaaat tcattcaagc aagtttccca ttttttagag
     1261 ccatctaaaa caagtaaaat attaaataat aaaccataaa gaattccttt atttaaatca
     1321 ctagcatcaa catctttacc ccaatcatag tgaacaggat attcagctgc ttcccataga
     1381 ggagatcctt cttcattaga taaacaaata gtcattgctc caaattctct tgcttttttt
     1441 gttgcttcta cagtttctgg tgttgtgcca gaatgggaac aagtgataac taaagaattt
     1501 tcagataatc cttttggtat agcatagtta aattcgttag cagtataaac atgacaaggt
     1561 acagttaatt ctttatcaaa tagatattga ccagccatta aaacggcttg tgaaccccca
     1621 catgctgtaa aatatacatg atcaattgtc ttttccttta acgctgccat tgctgtttca
     1681 atctgttctt tgtgtagttg tctcatttct ttatccctca ctctaataag tattataaag
     1741 tcatataact ttacaatacc tattatctat gaaattgctt tattttgcaa tactattttt
     1801 caaaaaaatt tttttataac aattttatat aataaaatg
//
LOCUS       DAZAJZ010000181         1773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000181 DAZAJZ010000000
VERSION     DAZAJZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.189"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACG86D_002931"
     CDS             <1..1604
                     /locus_tag="ACG86D_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN3141991.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DAZAJZ010000182         1764 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000182 DAZAJZ010000000
VERSION     DAZAJZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.190"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..413
                     /locus_tag="ACG86D_002932"
     CDS             <1..413
                     /locus_tag="ACG86D_002932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141992.1"
                     /translation="LFGGIIMLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNC
                     NEVIKRKLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRF
                     YNLSGNICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(586..789)
                     /locus_tag="ACG86D_002933"
                     /pseudo
     CDS             complement(586..789)
                     /locus_tag="ACG86D_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            755..952
                     /locus_tag="ACG86D_002934"
     CDS             755..952
                     /locus_tag="ACG86D_002934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141993.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(995..1570)
                     /locus_tag="ACG86D_002935"
     CDS             complement(995..1570)
                     /locus_tag="ACG86D_002935"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN3141994.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
BASE COUNT          591 a          291 c          250 g          632 t
ORIGIN      
        1 gtttatttgg agggattatt atgttagatg atagattgac gtgttctaat tgtggtacat
       61 tattttacta tgaggaagtt agaaatattg taaaacatag aaaaaaagaa atgcctatac
      121 gttgtccaaa ttgcaatgaa gttattaagc gaaagttgtc tcatggttat tttgtcagct
      181 acgaactata tgaagaaagt aaaataaatc tgaaaaaaaa accaaaatac tataaaaatt
      241 tgaaaactgg agctattata actgaaaaag aatttaaaaa tttagtgact gaagaagaaa
      301 gcaggtttta taatttgtct ggtaatattt gccctggatt tatggatcag attattgaaa
      361 atgagtcaat tgaatcaatt gaaaactatg ttcctgttga cttaccaaaa tagaaaaaat
      421 aatcctgttt ttacagtgaa ttattttaat tttgattttc aataaaaata tattacaata
      481 ataatcgtaa tagctaatga aacaatttct caatcttttt accactccaa acagggagcg
      541 tcaataattt tgtgtaaata actcgtcctt ctgcaaaata atgggttact cagtaaacat
      601 tgaagctaat gtatccgtca cttgttggaa gcctttatgg cttctattta agaatttttg
      661 attgtatgta tcaaagatgg atactaggaa acgctctagt gattcttcat tttgaaactg
      721 ctcttttctg cggctgtatt tcttgatttg tttattgaaa gattcgattg tagaaccaga
      781 aggaatggag aacacttttg aactttacaa caaaactact gttgataaat taattccaat
      841 tttagttcca actgatttaa atggtcatga tgttttactt cgttttaacg gatttttagg
      901 tgaggattat agatacctaa aaatttctga tcctcaacct ttaacagaat aataaaaaac
      961 gtgtaatcaa ttatttgatt acacgttttt tatttcaatc ttcgttgtag tagtggttgc
     1021 gacataccga aaggtaatct tcattacctc ctactacaat ctgttcacct gaataaactg
     1081 gaataccatc aacaatacga gcattcatta cagccttttt attgcaaaaa tggcaaatag
     1141 ttttaatttc ctcaatttta tcagcaaatg tcaataatgc ttcggatccc ttgaagagat
     1201 tattttgaaa atcattcttc aatccatagc aaattactgg tacttcctca ttcattacga
     1261 taagtgatag atcaataata ttctctctac ttaaaaattg agactcatcg accaaaatac
     1321 aatcaacttt tccgtactga tcaataatct ccactaattc tccgtacaca tccatttcag
     1381 aagtaattgc aatagctggt ttcgaaacac ctatacgact ctcaactttt ccaaatcctg
     1441 atcgagtatc tattgcgctt gtcattatga taactctctt cccttgttct tcataattat
     1501 gagccacttt cagtagctca atactctttc cactattcat tgttccatat ctgtaaaata
     1561 attgtgccat taaatttatt cccttctttt acaattcaat tattccttaa ttccatctgc
     1621 ttttatgcag ctgctcgttt attccttgat atgtctataa catctactat ccctaaaacc
     1681 aatttataag ccattagaga tggtattcct gaacatctct catattgttt tttgagtggt
     1741 atatcatctg aaaaactgaa ggaa
//
LOCUS       DAZAJZ010000183         1683 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000183 DAZAJZ010000000
VERSION     DAZAJZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.191"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..399)
                     /locus_tag="ACG86D_002936"
     CDS             complement(<1..399)
                     /locus_tag="ACG86D_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3141995.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQ"
     gene            complement(396..1049)
                     /locus_tag="ACG86D_002937"
     CDS             complement(396..1049)
                     /locus_tag="ACG86D_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3141996.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1337..>1683
                     /locus_tag="ACG86D_002938"
     CDS             1337..>1683
                     /locus_tag="ACG86D_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN3141997.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
BASE COUNT          539 a          289 c          193 g          662 t
ORIGIN      
        1 ttgcatatca gctgtagaaa caaattgtag atttgttttt ttaccatcat taagctttat
       61 ttctctttct tcaattatca aagaattact atcactttct aaattaacat ttatattatc
      121 tttgatttgt atttcattct tattattttt ttcttttatg aatactttta tttcactact
      181 cttggaataa aactccaaag tttgttctat atattttatt tcttttccat tcatattact
      241 agaaatttca tttgccatat tatgaatctt ttcttttctc gtctccaaat atgttttagg
      301 aaagataaaa aaaattaatg aatgaactaa tataattatt attccaagaa cagaaaatat
      361 ttgtatgaac atttttggaa atatctttaa ttttttcatt ttctctccaa tttatatcct
      421 acatttctta tagtagttat acagtctaat tgtaattttt ttctaagttc ttttatatat
      481 acatctatta ctcgatcata aggagtttct tcgctatctt tccatacata atcaataatt
      541 tgcattcttg ttaacacttg tccattatta tccaataaac attttaatac ttctagttct
      601 tttgcattta catctatttc ttcaccattt attttagctg tatagctatt aaaattaacc
      661 gatagatcct tatattcaaa cttctctaaa tgtccataat tcttcttaat taaagaatct
      721 attctagctt ttaagaccgg caatgaaaat ggcttttcta catatccgtc tactaaatta
      781 gtaaatgcat caattttata ttcttcatca ctaaatgcag tcaagattag aattggtaaa
      841 ttgcttttct ttctaatttc tttcaacact tctaaaccat ttataaaagg tatctgaatg
      901 tctaagataa ccaaatttat atcgctatta aattttgaca aggcttctct tccatcttta
      961 gcttgaataa cagtatatcc aaattctgaa agatattcac ttattccctc tcttatcata
     1021 ttatcatctt caacagttaa tattttcata tatacctcca cttaagccta tacactagat
     1081 attatacagc atattatggc tctataatat ctagtgttgg tgacaccttc tgttgccaat
     1141 actttttctt ttttctaata aatgcaaaca gacaccttga aatcctttag aataaagggt
     1201 tctataatat tttgtgttgt tggaaaatcg aaggcgattt tctactaaca ctttttcttc
     1261 gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa catcacccta
     1321 ccaaaggagt cgctctatgg ataatcatac tagaaaatta ctcgtattaa cagataaaca
     1381 tttatttttt gatgaagaat ggctgattga aaaagaatat aaaggcgttc aagcgcagtt
     1441 tattaaaggg agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat
     1501 tatccgcaac ggttcctata cgacacgtac tcagatattg aaagtcaaag aaaagttgac
     1561 tattttagaa ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc
     1621 gaaaacggac ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct
     1681 cat
//
LOCUS       DAZAJZ010000184         1676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000184 DAZAJZ010000000
VERSION     DAZAJZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.192"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..1009)
                     /locus_tag="ACG86D_002939"
     CDS             complement(125..1009)
                     /locus_tag="ACG86D_002939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3141998.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1104..1541)
                     /locus_tag="ACG86D_002940"
     CDS             complement(1104..1541)
                     /locus_tag="ACG86D_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3141999.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT          538 a          339 c          255 g          544 t
ORIGIN      
        1 atttttctat taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga caaaagtctt
       61 gtcacagtcc cttatcccga acaagcggtg ttctcccgcc aactcctcgg tcacaacctc
      121 cattttaaca ataaacctgt aaatgtgagg gcaatacttt gattgtcaca ggtaatttat
      181 ccccttcatc tccatcaaca ttcgtcgtca attcttcctc atcagctaat gacacaacta
      241 attgctcaca agtaaggtag gctagatttt tcgtagattc atctactcct tttaataggt
      301 ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt taaataaacc aaatggagct
      361 taccatcatc cacttttgca tttggaagta atgtttcaaa accgccgact gagttagtta
      421 gtcctatcag caaggtactg ctctcaattt cttccttctt tccatcaact tctacgtgaa
      481 acgagtagga ttgtgtggaa gctaactgtt tgattcctga catgaaataa gctagttttc
      541 caaatttcgt cttcttctcc gcatctacat cattgattga ttcagggata gagccaattg
      601 cgacgacatt cataaaatag tcgtcattta tttttccaat atccaatggc ttgactgatt
      661 caatcgaaaa atgattgatc gcttcctcag gttctagcgg tatccctaat gctcgagcca
      721 agtcattcac cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct tgttcagcga
      781 ttccactgat tccttcgttg actgttccat ctccgcccat gacaaacaca ctatgatacc
      841 cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt gtatgcaaga
      901 cgaccacttc atcaaacacc gattccaatt ttgctatcgc taattgctca aattcctttg
      961 cttgttcacc gcctgaactg gggtttacca ctaataaaac ttttttcatc actttctttc
     1021 ccttctgatt gatttcttta atataacgat agcataaaga aaaaaattag gctgtgatga
     1081 agtactgttc atgaaacatt catttatttt ttgacaagat aaaacatcca tactatgaaa
     1141 ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa atgataggca ttgtatacct
     1201 aataaagtaa aaatcttagg tggcagataa aaacacaaaa atagcaagag aatgtaatag
     1261 cgtttcttcc actttttgtt atcagacctt atagcgatca gaggaatacc aaatgcataa
     1321 ctgaaaaact cttgatgtac tgttaatatg tcacggcctt gaataataaa tgcgacaatc
     1381 cccgctataa ttagtgtgcc taacaccaaa acggaaaatt gatgaatata ttttatatac
     1441 aaatgatcca ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc gtatacgtga
     1501 ataacttcta gaaataaaag aaatttgcaa aaccactcca tttatctact tctacgtgca
     1561 ttttttgata ttgttttctt tatttaagat tatacagaaa aatatctagg gtcgctggat
     1621 tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc aagtagtaat taaact
//
LOCUS       DAZAJZ010000185         1588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000185 DAZAJZ010000000
VERSION     DAZAJZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.193"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1542
                     /locus_tag="ACG86D_002941"
     CDS             76..1542
                     /locus_tag="ACG86D_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN3142000.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          441 a          291 c          333 g          523 t
ORIGIN      
        1 ataagttttt tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga
       61 attggggtat agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga
      121 ttgtcaactt tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca
      181 ttacttatct attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca
      241 acagcattag ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc
      301 ggatgggtat ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc
      361 attatgcttg gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg
      421 atggcattga ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga
      481 catctttatt caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt
      541 gtcaacttag gggcattatt agcaccacta atcgttggta caatcggtca agaatataac
      601 tatcatttag ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc
      661 atccaaggac gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact
      721 gaagaacgta aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta
      781 ttcggcgggg cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt
      841 gtattgggga ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta
      901 actgctgaag aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt
      961 ttctggtcat tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca
     1021 caatctacgc tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt
     1081 ttcatcttta ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa
     1141 ccatctacag tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta
     1201 ttgatgttgc cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg
     1261 ggaagtttct tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg
     1321 acaacaaaat tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct
     1381 gatgcttctt cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa
     1441 tctgcttact tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact
     1501 gtgaaagaac caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca
     1561 ggaattgctt atttgcagca gttcctgt
//
LOCUS       DAZAJZ010000186         1568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000186 DAZAJZ010000000
VERSION     DAZAJZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.194"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..593
                     /locus_tag="ACG86D_002942"
     CDS             183..593
                     /locus_tag="ACG86D_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3142001.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            607..963
                     /locus_tag="ACG86D_002943"
     CDS             607..963
                     /locus_tag="ACG86D_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3142002.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          582 a          220 c          280 g          486 t
ORIGIN      
        1 ctcccttatt tattttgaaa atgagaatac tattatcaaa atagagaata tatagaaaaa
       61 ttctcattat tcaaacgtaa ataatcagtt attttgattt tttttatatt ttattagcta
      121 ataattggta ttttaaaaga aattactgta gaattggatt ataattaaaa aggagtgaga
      181 aaatgataaa agtatatact tcgcctagct gtacttcctg tagaaaagca agagcatggt
      241 tgcaagcgaa tcaacttgaa tttgaagaga agaatatttt tgccgaaccg ttaacagaaa
      301 gtgagatcaa aaagatcttg gcagcgactg aaggaggagt tcaagatatt atttccactc
      361 ggtcgaaaat ttacgaaaag ctgggcatag attttaacga attaacttta aagcaaatga
      421 tcgtcttatt caaagaatat ccttcattat tgagacgacc tattttgatt gatgaaaaaa
      481 gaatactaat cggatttaat gaagatgaga tccggtcatt tgtccctcgt gaaattcgat
      541 gtgttgccag aaatctgatc tatttacaaa acaataacat cttaaattca tagggaggga
      601 gccttcgtgg aagtgaaagt ctatggatca gcgcactgtg taacaagtct gaaggcgaaa
      661 caatggttag aacaccatca attgccttat caatttgttg acttagaaga acgcgattta
      721 agtacagaag aagcagaaga attatgcagt tttgaagaag tgcaagcaga acagctattt
      781 gcgacttggt ctgaggcttt tcacaacatt gcgattgacc tttcctgtgt aggaaaaaaa
      841 caactgattt ttttatgcac acaacatacc aatctcctca gacgtccttt gattattatc
      901 gatgaagttc tatttattgg atacaatgag cagttgatgg aacagcttat cttaaataaa
      961 tagactttaa ctgttttaat caagtcactg agcatttcat acagttgtaa cagtcgaaca
     1021 cttagcatca agcgtgttat gtgttctttt tttgacttta tttttctact atagagacat
     1081 gctcaattta tgtggtcaaa attcaaatct aggtggggat atagagttaa aaatatattg
     1141 acatgtgtaa atttacatga tatattcatt tctgttgttt taagtagcaa agcaataaaa
     1201 tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca tgataagata
     1261 actgagttgt taaaaaagaa ccacttgtga agcttgattc tatcgaaaaa aacttcaaaa
     1321 aagttcttga caaataacaa atgataagtt atgataaatg agttgctgta agatttcgaa
     1381 aaacgaaata aaaaaaactt ttaaaaaaga gttgacaatt acttgtctct ctgatatgat
     1441 ataaaagttg ctacggcagc caaaaacgaa tcggggaaca ctcctgatga agtagacctt
     1501 tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc aattcaagac
     1561 aacaaaca
//
LOCUS       DAZAJZ010000187         1525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000187 DAZAJZ010000000
VERSION     DAZAJZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.195"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(497..1111)
                     /locus_tag="ACG86D_002944"
     CDS             complement(497..1111)
                     /locus_tag="ACG86D_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3142003.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          501 a          288 c          245 g          491 t
ORIGIN      
        1 atttcattgc aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt
       61 tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta
      121 aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga
      181 agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt
      241 ctctttttcc actacttttt atttttctga cactacatat tcttataact catttctcat
      301 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
      361 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      421 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      481 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      541 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      601 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      661 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      721 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      781 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      841 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      901 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      961 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
     1021 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
     1081 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
     1141 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
     1201 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
     1261 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1321 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1381 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1441 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1501 caagtgcaag caagaaaata caaaa
//
LOCUS       DAZAJZ010000188         1522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000188 DAZAJZ010000000
VERSION     DAZAJZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.196"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            767..1153
                     /locus_tag="ACG86D_002945"
     CDS             767..1153
                     /locus_tag="ACG86D_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3142004.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1247..1438
                     /locus_tag="ACG86D_002946"
     CDS             1247..1438
                     /locus_tag="ACG86D_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3142005.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          567 a          234 c          209 g          512 t
ORIGIN      
        1 aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat aagacttctt
       61 tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag tattttatac
      121 gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta taaaatatcc
      181 acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg agaactcatc
      241 atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat aattattatg
      301 atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta aatattccaa
      361 tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc ttttctagtt
      421 gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa gaatattatc
      481 caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat tttcttagcc
      541 ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag tataaagata
      601 actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg
      661 catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt
      721 tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga
      781 cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa
      841 tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc
      901 atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac
      961 tctccttgtt gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca
     1021 aatttttctt atcgaaaaat ttatctttga taaaactgat aaagaaattg gaaaggttct
     1081 ggggattaca agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa
     1141 aatgctggtt taatatctgc ataataacta attttgtatg cttctttgct cgttctcctc
     1201 cttcccaaag aaatagatta taagtccatg ggaagaaggt agatttatga attctttaaa
     1261 agaagatttc attttagcaa aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag
     1321 atttagctct ttgattcata agcaatcctg gagaactgga aaatatgatc aagattgtta
     1381 tcaagaatgt atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta aagaataact
     1441 caactaaggg agtctgtgac ataagtaaaa agatagtttc aaatttcgaa caataaattt
     1501 gaaactatct tctttataat ta
//
LOCUS       DAZAJZ010000189         1507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000189 DAZAJZ010000000
VERSION     DAZAJZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.197"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..918
                     /locus_tag="ACG86D_002947"
                     /pseudo
     CDS             70..918
                     /locus_tag="ACG86D_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1271..>1507)
                     /locus_tag="ACG86D_002948"
     CDS             complement(1271..>1507)
                     /locus_tag="ACG86D_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3142006.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          480 a          271 c          246 g          510 t
ORIGIN      
        1 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
       61 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
      121 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
      181 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
      241 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
      301 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
      361 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
      421 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
      481 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
      541 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
      601 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
      661 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
      721 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
      781 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
      841 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
      901 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
      961 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
     1021 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
     1081 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
     1141 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
     1201 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
     1261 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
     1321 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
     1381 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
     1441 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
     1501 tcgcatc
//
LOCUS       DAZAJZ010000190         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000190 DAZAJZ010000000
VERSION     DAZAJZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.198"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG86D_002949"
     CDS             64..972
                     /locus_tag="ACG86D_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN3142007.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACG86D_002950"
     CDS             972..>1480
                     /locus_tag="ACG86D_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142008.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAZAJZ010000191         1447 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000191 DAZAJZ010000000
VERSION     DAZAJZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.200"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG86D_002951"
     CDS             complement(138..428)
                     /locus_tag="ACG86D_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142009.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG86D_002952"
     CDS             complement(546..806)
                     /locus_tag="ACG86D_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142010.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG86D_002953"
     CDS             complement(943..1356)
                     /locus_tag="ACG86D_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN3142011.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          531 a          238 c          214 g          464 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctt
//
LOCUS       DAZAJZ010000192         1421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000192 DAZAJZ010000000
VERSION     DAZAJZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.201"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG86D_002954"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG86D_002954"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN3142012.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          496 a          184 c          236 g          505 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta t
//
LOCUS       DAZAJZ010000193         1421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000193 DAZAJZ010000000
VERSION     DAZAJZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.202"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..696)
                     /locus_tag="ACG86D_002955"
     CDS             complement(64..696)
                     /locus_tag="ACG86D_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN3142013.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1047..1346
                     /locus_tag="ACG86D_002956"
     CDS             1047..1346
                     /locus_tag="ACG86D_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3142014.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          510 a          199 c          227 g          485 t
ORIGIN      
        1 ataggcaatg tttttttatg gatatttgta ttgctaaaaa tttatcttat atattcattt
       61 tgattatact acttttattt gtaaaaattt tctagcaaaa ccaagtgctt gcaactgatt
      121 aagcgtacaa ttttttagat gttccgagcg tacttcaatc atttcaaagg agttgctact
      181 taaatcgata ttttttaaat cagtatcaaa aaagctagca ttttctaaat cattagtaga
      241 aaacattatt ttagtgaggg aacagtctac aaaatctgcg cttgataaga cagagtgctc
      301 aaaggtactt tcttttatgt ttgaaccaga taaatttaca tagtccattt tacaattctc
      361 gaataacgtg ttcccaatat aactttccat caatttagta cctgttaatt tacaattgac
      421 aaatttcacg cgatgcaaca tgctttttgt caatatacag cctgaaaaat cacagttttc
      481 gaatatacag tcggtaatct ccattctttg aaaatcattg gaagcaaaaa atgcatactt
      541 gaaaatagat ttgtaaataa ttggatggca gattatttgg ttttcgatga cctctttttg
      601 tgaaaggata ttatacagtt caaaatcttc gttttcatag atatgaaaaa aggatccttt
      661 attcaagctt gcttcattaa ctttaggaaa aatcatattt aattacctcc attttcataa
      721 agtcatatta acaaaagcta aaccaactta cattagcaga aaatgagata ggttttcgcc
      781 tttttcgata acgatagtgt cgctatctct tgaaacgttc ttagaattgc cctgagagac
      841 acaaattagg cgtaattcta ataaaaatct tagaataaaa atttttactg atttaattta
      901 gaatgttttt aaaataaaaa tcgttgccat aacagtgtat tgaaaggttt taacaatcca
      961 gtaatgattt aaaaaaatct agatgtagcg tatactttgt ttatagaatg tagtgggaag
     1021 aaatatattt ttgggaggaa atcgtgatgt ctaaaagtga agaaatagaa aacatggtac
     1081 taaaattata tgatctcgtt ttaaatccag agatcaaaga aaaggaacgt atacttttga
     1141 tagacgcaaa gacaggctta gaaaaaggtc agtattatcc taaagtcata aataatttgg
     1201 aacgtagttt acgtccgtta gcaatcaggg gagaattgtc taaaccagta tcaccgtttt
     1261 atatggagat atcaacaata ggaaaatttg aaaaagaatt aggtcgagga atggcttcgg
     1321 caccgattac ttttgggcat ttataaaaac gagttattca tctcaatgaa ttaaaaagta
     1381 tagtcaatct cataatgcag gctaggttca tttatttgaa c
//
LOCUS       DAZAJZ010000194         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000194 DAZAJZ010000000
VERSION     DAZAJZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.204"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG86D_002957"
     rRNA            <1..1355
                     /locus_tag="ACG86D_002957"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZAJZ010000195         1286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000195 DAZAJZ010000000
VERSION     DAZAJZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.205"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>1286
                     /locus_tag="ACG86D_002958"
     CDS             6..>1286
                     /locus_tag="ACG86D_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN3142015.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          431 a          250 c          247 g          358 t
ORIGIN      
        1 gctatatgaa tgattctatc aaaaaaatgc tgagaataac agagaaagat ttgatgatta
       61 cagaggtctc ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat gctgttctct
      121 cgcctactcc tcgtgcttgt agaagttgtg gttctactgt ggtagatgga aacgggaaag
      181 caattatagt gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa tacaatcata
      241 tgcctttggt tatgcgccta aaaaagcaac gctatacctg taaaaactgt cgcacccatt
      301 ggacggctca aagttatttt gtccaaccca gacattcaat cgcaaatcat gttagatata
      361 aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa agctgtcagg
      421 tatctttgac caccgttatt cgtacattga aagagtttaa aagctattta ccaaagcaat
      481 ctaagaagat cctccccaga gtattgatgg ttgacgaatt tcgttcgcat gcttccatag
      541 aagataagat gagctttatt tgcgcggatg gcgaaacagg aaaattaata gatgttttgc
      601 ctacgcgtaa attacctcga ttaacaagct atttcttagg ttgtaccaat ccagaagaag
      661 tagaattctt ggtgacagac atgaacgccg cctacttcca gctcaccaaa cgtgttctgc
      721 caaatgcgaa agtcgtgatt gatcggtttc atattgtcaa acacatgaat caagcgttca
      781 atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa agccaggcag
      841 aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc aaccattatg
      901 aatacaaaac atggaaaagt ttccgagcac caaaataccc atttcttact gaagcaatga
      961 tgattgatcg attgcttgag ttttctccgc ccctaaagga ggcgtatccc ttttttcatg
     1021 aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta ttggcagaac
     1081 ttcccgaaac gttagatgac agctttcggg aaaagcttca aaaccttctg acctatgaag
     1141 aaggcatcac caacgcaatg atctatcctt attccaatgg aaaaatagaa gcgaagaata
     1201 cccacataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag aacatgagaa
     1261 ttagaatctt tttgatcaat caatta
//
LOCUS       DAZAJZ010000196         1260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000196 DAZAJZ010000000
VERSION     DAZAJZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.206"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACG86D_002959"
     CDS             complement(93..1136)
                     /locus_tag="ACG86D_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN3142016.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          433 a          251 c          238 g          338 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
//
LOCUS       DAZAJZ010000197         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000197 DAZAJZ010000000
VERSION     DAZAJZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.207"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG86D_002960"
     CDS             200..742
                     /locus_tag="ACG86D_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142017.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG86D_002961"
     CDS             898..>1246
                     /locus_tag="ACG86D_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN3142018.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZAJZ010000198         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000198 DAZAJZ010000000
VERSION     DAZAJZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.208"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG86D_002962"
     CDS             414..947
                     /locus_tag="ACG86D_002962"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN3142019.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZAJZ010000199         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000199 DAZAJZ010000000
VERSION     DAZAJZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.209"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG86D_002963"
     CDS             <1..1076
                     /locus_tag="ACG86D_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN3142020.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZAJZ010000200         1162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000200 DAZAJZ010000000
VERSION     DAZAJZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.210"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..546)
                     /locus_tag="ACG86D_002964"
     CDS             complement(<1..546)
                     /locus_tag="ACG86D_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFN3142021.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVG"
     gene            complement(539..>1162)
                     /locus_tag="ACG86D_002965"
     CDS             complement(539..>1162)
                     /locus_tag="ACG86D_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFN3142022.1"
                     /translation="SSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFK
                     IFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFK
                     IGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSM
                     NKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          421 a          181 c          137 g          423 t
ORIGIN      
        1 tccaacattt ggacttgtat agacaccaaa taaccgatgt tcaataaaac cttgtcttgc
       61 aagtaatcct ggcgttcctg ttgggacatg ataagaaatc aaaaaaataa attgatagat
      121 agatattagc gcagctaaaa aaattatgat aatgaaaaca ttattaaaat acaccatttt
      181 tttctttttt tcctcttcac tttgtgaaaa gtctgcagga aaaattacta tcaaagtaat
      241 cagcaaatat ccaaaattat aaatagatgg aattaaaaaa ttttctctgt taatgattat
      301 agttaagata aaactaatca ttgctccaat cagtagccaa gaaaaacgca ttttaaaaaa
      361 atttttcttt ataaaaacct catacaaaac tataattcca ccccaagcaa aagctatttt
      421 catcgggata ttagttacat taaaagtact aggcataaaa taaagcagac aaatgattaa
      481 atttattact cgaaacgcat ctcgacttaa taaactcttc tctaaaacag gtactattct
      541 attcatctat atttccaact ttcaaaattt ctttatcaat tctatttaat agtttatcag
      601 agtaatccga tttaactttt cttacaaata atttttcact ttcttttatt tgatctatat
      661 ccctttcatc aaaccatttg ttcatggaag gatcaataat atgaatattt gctaatttcg
      721 aagtttgtaa agtagtttct tcaatatttt tcaattcaag tggtaaatct ggtcctccaa
      781 taacattttt atttttgaac gaggaattaa aaaaaagtgt atgaaaaaat ttttcatctg
      841 gggcaaacat acctatttta aaattcaggt agtcttgttg attttgttca tacatttgta
      901 ttaattctgt taggcattct ttcgacaaag cccaccattg agaaccatga tacaaatcaa
      961 atttatggtg tctaattttt gggggaagtt taatcatcct cagacaacga ttaattatag
     1021 ctctaaaaat tttaaatgca gttgtgttat tcctattaat tctaggataa tcatgttttt
     1081 gatatacatc tatatgttta taataaaatt ctttatgttt aatttgatct agatctattc
     1141 cacgaataaa ctccactgaa ct
//
LOCUS       DAZAJZ010000201         1146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000201 DAZAJZ010000000
VERSION     DAZAJZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.211"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..274)
                     /locus_tag="ACG86D_002966"
     CDS             complement(<1..274)
                     /locus_tag="ACG86D_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142023.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISS"
     gene            complement(291..>1146)
                     /locus_tag="ACG86D_002967"
     CDS             complement(291..>1146)
                     /locus_tag="ACG86D_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN3142024.1"
                     /translation="NLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTI
                     EQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTI
                     RSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVY
                     YTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHI
                     TIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGL
                     VDNFNNIS"
BASE COUNT          394 a          196 c          129 g          427 t
ORIGIN      
        1 aacttgatat tttagcatct agcttaatca gattaaataa tgtttgttta aaattgctac
       61 cattaatatg actgatagat tctctagctt cttcacataa actttttact ttattaatta
      121 ctactttgtc atattcattt aacacactat tattaccatt acttagacgc tcagattcta
      181 cgaaacaact caaatcagaa atttcccaaa aaaatttttt gttaaaatcc aatttcctca
      241 actcctccaa actacgtaaa tgatatagtc tcataataaa ataaaacctc tcagctaatg
      301 ttgttgaaat tgtccactaa acctaattga tattgcttag gagatacacc tgcgtacttt
      361 ttgaatactc tcgaaaagtg tgaagaatct gaaaatccta aacctaaaga aacttcactg
      421 atagtatggt ttgatttcaa cattatttta gccgtagaaa tttttcgctt agagatataa
      481 ttataaatcg taatatgcat ttcattctta aagctttctc ttaatttagt ttcacttaca
      541 gaaaaataat tagctatttc ctttacagta attcttttat agatatttaa gccgatgaat
      601 tgaacgacag aagaaattaa gggagaaaga tgctctatct tagtttttcc gatttcctta
      661 gtataataaa caatcccaga atccctcact tttaagacct caggtaacga tacagctaat
      721 tcgttttctt gtatcaaagc atctctactt tgatacgctt ctattatatc cattcctaat
      781 gttatagacg tttgtgctaa cttttcaaaa acaatgatag aataattttt ttcagaccga
      841 atcgtatctc cactaacact tggaattatt ccattactca gattaaaaac catgttttct
      901 aaatttcgaa tatctccatt tttaacatat tccagtattt tattttcata aagaaacaga
      961 tatatttctg gatcgtaatt ttgtgataac agattatcta ttttgatttg ctcgatagtt
     1021 ttacttagat tttctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca
     1081 gtaaaaaaat aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc
     1141 aaattt
//
LOCUS       DAZAJZ010000202         1127 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000202 DAZAJZ010000000
VERSION     DAZAJZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.212"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..88
                     /locus_tag="ACG86D_002968"
     CDS             <1..88
                     /locus_tag="ACG86D_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN3142025.1"
                     /translation="FKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            93..1040
                     /locus_tag="ACG86D_002969"
     CDS             93..1040
                     /locus_tag="ACG86D_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN3142026.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          360 a          170 c          239 g          358 t
ORIGIN      
        1 ttttaaatgg ttagaatttt tgtttcctaa atttttagaa gattcttcgg agcttcaaga
       61 tatctataat tatttatatc ggaggtaaaa ggatgagctg ttatcttatc accggtgggg
      121 ctggcttcat tggttctacc ttagccaatc atttaggaga agaacatgtg gtaatcgtcg
      181 tcgatgactt atcaatggga aaagtagaaa atcttgatat gacgaaaaat atcactttta
      241 ttgagggaga tgtcgctgag aaatccttga tggaagagat catgaaagct aataaatttg
      301 attatatctt tcatttagca gcagttgcta gtgtggcaga ttcagttgcc cgaccagtag
      361 aaacacatcg tgtgaatttt gaaagtgtat taatgatttt agaattgatc cgcaagtatc
      421 aacctgattt aaaacggatc gtgtttagtt cttcagcggc tgtttatggg gatgaaccga
      481 ctttaccaaa aaaagaagag tccgtgattc gcccattgac gccttatgcg gttgataaat
      541 tcgctgcaga acaatatgtg ctagattatt gtcatttata tgatgtaccc ggtagcgcag
      601 ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa ttctccatac agtggtgtga
      661 tttctattct cgtagatcgc tataagaaac aaatcgtagg agaagcaact tcatttacat
      721 tatttggtga tggaagccag tctagagact ttgtttatat agatgatgta atccaagcat
      781 tacttttagt tgcgaatgag gaagctgcac tggggaaaca attcaatgtt ggaacgggta
      841 aagcaaccac acttttggaa ttgattcatg caatcaatga aattctcggt gtagaattga
      901 cacttgaata caaagaagag cgttcgggtg atatccatga ctctctagca gatattacta
      961 agatccagtc aattggctat caaccgaaat atggtatttt aagtggaatg actaattatt
     1021 tgaaaactga aatcaattaa aacatactaa gataagatta gagggtaaat tcaggctctt
     1081 tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg gatagaa
//
LOCUS       DAZAJZ010000203         1065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000203 DAZAJZ010000000
VERSION     DAZAJZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.213"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>1065)
                     /locus_tag="ACG86D_002970"
     CDS             complement(38..>1065)
                     /locus_tag="ACG86D_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFN3142027.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          178 c          160 g          381 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caact
//
LOCUS       DAZAJZ010000204         1022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000204 DAZAJZ010000000
VERSION     DAZAJZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.214"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..461
                     /locus_tag="ACG86D_002971"
     CDS             3..461
                     /locus_tag="ACG86D_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFN3142028.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            470..697
                     /locus_tag="ACG86D_002972"
     CDS             470..697
                     /locus_tag="ACG86D_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3142029.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          167 c          180 g          356 t
ORIGIN      
        1 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
       61 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
      121 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
      181 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
      241 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
      301 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
      361 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
      421 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
      481 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
      541 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
      601 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
      661 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
      721 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
      781 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
      841 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
      901 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
      961 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1021 gt
//
LOCUS       DAZAJZ010000205         1003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000205 DAZAJZ010000000
VERSION     DAZAJZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.216"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG86D_002973"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG86D_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZAJZ010000206          998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000206 DAZAJZ010000000
VERSION     DAZAJZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.217"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..901
                     /locus_tag="ACG86D_002974"
     CDS             2..901
                     /locus_tag="ACG86D_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142030.1"
                     /translation="MNFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQKSSERFMS
                     NQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFANSFWFLKN
                     INKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIM
                     SQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLK
                     LAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIG
                     TLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          405 a           97 c          174 g          322 t
ORIGIN      
        1 gataaatttt gatgtggata aactttataa aagtttgata gttgttccca aaaataaatc
       61 agaagatggg aattatgagt tagaagtttt gcaatttaag attgaacaaa agtccagtga
      121 aagatttatg agcaatcaat tagtgaaaat caaaaaatgg ctccctatga ttgatcaatt
      181 agtatctaat aataaggaag ctaaaaattt atgtattttt gtcttattat tacatggaag
      241 agttattaac ttagttaatc gaaaaagtac gactaaattt gcaaactcat tctggttttt
      301 aaaaaatatt aataaaacag tggaaaagaa tggagtttat aaaggtgatt ttcattttca
      361 aaatgctgag actattgaac gagaacatca ttatttcgat ttattagtga atacagtggt
      421 agaattagcg aaatgggcaa agtcaaacga aacttatagt caagtactta aaagaattat
      481 gagtcaagaa acttcattta tctatgagtt aagcaagtca aatttgaaat atacttataa
      541 tgaagatgaa cttgaaattt tagataatcc agaaaattca caatggatta aagagttgaa
      601 gagagaagac tttattgaga cactaagaat tctaaaatcg ttatttgatt tgaaattagc
      661 caaagctacc atcatgggaa ttgaaagaga ttcatattat tgggaggaaa ttggactata
      721 tgctccccag attgaaggtg aagataaaga gaaaaggata gacaacatta ttaactatat
      781 taatagttta agtgaacctt taaaatgtag aggacttgat tttataggaa cattaggatg
      841 gacagaaaca tactatgaga aaatatataa agaaataatt gaacaatatt ttcctaaata
      901 atttaataag gattgattta tcgtaaaaga aaaaagttgt gatttccaaa atcacaactt
      961 ttttcttttg tcaagatttt gagtgggata tatttttg
//
LOCUS       DAZAJZ010000207          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000207 DAZAJZ010000000
VERSION     DAZAJZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.218"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACG86D_002975"
     CDS             <1..>986
                     /locus_tag="ACG86D_002975"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN3142031.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAZAJZ010000208          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000208 DAZAJZ010000000
VERSION     DAZAJZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.220"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG86D_002976"
     rRNA            <1..>943
                     /locus_tag="ACG86D_002976"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZAJZ010000209          922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000209 DAZAJZ010000000
VERSION     DAZAJZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.221"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>922
                     /locus_tag="ACG86D_002977"
     CDS             <1..>922
                     /locus_tag="ACG86D_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3142032.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLNN"
BASE COUNT          280 a          187 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca ac
//
LOCUS       DAZAJZ010000210          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000210 DAZAJZ010000000
VERSION     DAZAJZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.223"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG86D_002978"
     CDS             <1..888
                     /locus_tag="ACG86D_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN3142033.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZAJZ010000211          877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000211 DAZAJZ010000000
VERSION     DAZAJZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.224"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..877
                     /locus_tag="ACG86D_002979"
                     /pseudo
     CDS             1..877
                     /locus_tag="ACG86D_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          335 a          147 c          162 g          233 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
      661 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
      721 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcgatcattg agtcataaag
      781 gctgtccttt tgataatgcc gtagctgaat caacttataa gtcgttgaaa gtagaatttg
      841 tctatcaata cacatttgaa accttacaac aattgga
//
LOCUS       DAZAJZ010000212          872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000212 DAZAJZ010000000
VERSION     DAZAJZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.225"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>872
                     /locus_tag="ACG86D_002980"
     CDS             <1..>872
                     /locus_tag="ACG86D_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN3142034.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYV"
BASE COUNT          295 a          149 c          178 g          250 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gt
//
LOCUS       DAZAJZ010000213          867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000213 DAZAJZ010000000
VERSION     DAZAJZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.226"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>867
                     /locus_tag="ACG86D_002981"
     CDS             <1..>867
                     /locus_tag="ACG86D_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFN3142035.1"
                     /translation="NSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSN"
BASE COUNT          318 a          110 c          152 g          287 t
ORIGIN      
        1 gaattcttgg atttatcaaa tccaattaac atcggacgat aaattatatt ccttcatcaa
       61 tgatattaat agttggtgta gtcgagaagt tcaattagtc aatcgtttta ttcaaatagg
      121 ggatatttat ttaacaaaaa ttcaaaaaaa tgttttggtt attttattta agttaatgtg
      181 tagtaaagaa tttcagcaaa aacgtttagc actgattttg gctcatggag atctaacggc
      241 aacctgtata gcaagtactg ctaataggat cgttgagaga tatatatacg acgccattga
      301 tatgccacta atgacttcaa gtagtgagat tgcagacaaa attaatagat acttacaaga
      361 gtgtaccgga tatgaagaaa ttattttact tgttgatatg ggttcactgg agaaaattta
      421 tctaaatttg gaactagacg ctcaaaaaaa attcggacta ctaaacaatg tttcaacaag
      481 aatggctatt gatgttgcaa atcagatatt attagattta ccgcttaaac agattcttga
      541 acaagtaaaa gaaaatcaaa atgtggatta tatttatgaa tcatcaaata ttaagaagga
      601 tatgattgtc tgttcttgtg caagcggatt aggaacttcg tttaaattaa aaaggatact
      661 tgaagatagc tttccaaggg atactgatct tgtcatagaa acgtgtgact attactcttt
      721 agtgaatgaa aactatttat cggagcttcg taaagagaac aatattcttt ttattgttgg
      781 aacactaaat ccacatctag aagatgtaga attcttttca attgaagata tgattttagg
      841 aatgaatata agaaaattct ccaatca
//
LOCUS       DAZAJZ010000214          843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000214 DAZAJZ010000000
VERSION     DAZAJZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.227"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACG86D_002982"
     rRNA            complement(<1..>843)
                     /locus_tag="ACG86D_002982"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAZAJZ010000215          822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000215 DAZAJZ010000000
VERSION     DAZAJZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.228"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..683
                     /locus_tag="ACG86D_002983"
     CDS             <1..683
                     /locus_tag="ACG86D_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN3142036.1"
                     /translation="RHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQK
                     IDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQ
                     RTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWY
                     GRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGL
                     RLHSMGLA"
BASE COUNT          300 a          130 c          153 g          239 t
ORIGIN      
        1 cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa attatcgaat
       61 tacctaaaac taattccaca attggtattg acttaggtat tatggacttt gcgattcttt
      121 ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag aaactaaagc
      181 gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt accaatctat
      241 ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat gaaaaagtaa
      301 tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa aatcacgata
      361 ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa ttagctaaaa
      421 gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag gctgactggt
      481 atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt tgttcagagt
      541 gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt cctgtttgtc
      601 atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa ggtctaagac
      661 tacattctat gggattggct tagatccaat catcaagaac cgtaggaact acggggatag
      721 cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta tgctctattc
      781 ccaagaagct cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DAZAJZ010000216          812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000216 DAZAJZ010000000
VERSION     DAZAJZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.229"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>812
                     /locus_tag="ACG86D_002984"
     CDS             <1..>812
                     /locus_tag="ACG86D_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFN3142037.1"
                     /translation="DFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEK
                     YDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTI
                     IQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFM
                     DATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLE
                     EVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSD"
BASE COUNT          277 a          118 c          150 g          267 t
ORIGIN      
        1 gattttacta cagaaattat ggaaatacta atcaataaag gtgatttgga tgatttgttt
       61 cgtcgtcacc tagaattggc tattaacgct ttattacaag ctgaattaac ggtatttctt
      121 gactatgaaa agtatgatag agctggattt aattcaggta attcccgtaa tgggaattat
      181 tcgcgctctt ttaaaacaga gtacggtgaa ttgaatttag taatacctag agataggaat
      241 ggagactttt ctcaacaaac tttacctgct tataaaagaa ccaatgactc tttagaaacc
      301 actattattc agctagttca aaaaggaatt actatgtccg aaatctctga tctaatcgaa
      361 aaaatgtatg gtcattatta caaaccacaa actatttcaa atatgagtaa aattgtgtct
      421 gaagatgttt cggcatttaa agaaagaact ttagaagcta aatactcagt catttttatg
      481 gatgctactc atattccctt aaaaagacaa accgtatcaa aagaagctgt ctatatagta
      541 attggtatca ggttggatgg aactaaagag gttctaggat ttactatagc tccaactgaa
      601 tctgcttatg tttggaaaga gattcttcaa gatctaaaag accgtggttt agaagaggtt
      661 ttgttagtcg taacagatgg tttaagtggt attgacgata gtattcatag tgtttatcca
      721 aatgctcaat ttcaacaatg ttgtgtccat atatctagaa atatcgctca taaggttcgt
      781 gttagtgatc gacaagaaat ctgtagcgat tt
//
LOCUS       DAZAJZ010000217          797 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000217 DAZAJZ010000000
VERSION     DAZAJZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.230"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..>797)
                     /locus_tag="ACG86D_002985"
     CDS             complement(27..>797)
                     /locus_tag="ACG86D_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN3142038.1"
                     /translation="YITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKW
                     MVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSS
                     DNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRA
                     HGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSI
                     FIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          303 a          143 c           97 g          254 t
ORIGIN      
        1 tccaagcaat gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg
       61 cttcccaaat aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga
      121 gcctccacca tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac
      181 gcataattca ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag
      241 aaagcataga tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca
      301 ttatgcatgt ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact
      361 tttttcaaaa attttgcatc gctattccca tagaaatcgg tctctccata accaaataac
      421 aaagttggtc ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt
      481 tcggagttat cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct
      541 gattcatttg actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa
      601 aaatccttag cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt
      661 accatccatt tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg
      721 gcaaacaaca aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac
      781 gaagaaagtg taatata
//
LOCUS       DAZAJZ010000218          748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000218 DAZAJZ010000000
VERSION     DAZAJZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.231"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG86D_002986"
     CDS             33..656
                     /locus_tag="ACG86D_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142039.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          269 a          113 c          156 g          210 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaa
//
LOCUS       DAZAJZ010000219          740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000219 DAZAJZ010000000
VERSION     DAZAJZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.232"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>740
                     /locus_tag="ACG86D_002987"
     CDS             2..>740
                     /locus_tag="ACG86D_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142040.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELL"
BASE COUNT          232 a          105 c          111 g          292 t
ORIGIN      
        1 catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt taagtttaga
       61 atctattttt agctccccta ttaattttga aacgtttaga tcatatggca tgcaagtaaa
      121 taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt ttactaattt
      181 agcaataggc tataaccttt ttctttatat tgttacgctc attactctat ttatatgtca
      241 ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg ctattatata
      301 tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg gggaattact
      361 cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg actcaaaaaa
      421 agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc atgttctttc
      481 tgtagctatg actatggtaa tgattataat tgctttaatt attcaatttt atcggaaaaa
      541 gaaaatagaa atcttcttaa ttattaaatt aataaaagct gctatagtta ctttacttct
      601 ttcactagcg tttataggtc ctatgattga gcagacattg tatcaagatc tgaatagacc
      661 ttatgtggat atattacaaa aaagagcaat ttatctgggt aaagagtttt tgattggaag
      721 tatagattca gaattactat
//
LOCUS       DAZAJZ010000220          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000220 DAZAJZ010000000
VERSION     DAZAJZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.234"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG86D_002988"
     CDS             complement(54..629)
                     /locus_tag="ACG86D_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN3142041.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAZAJZ010000221          662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000221 DAZAJZ010000000
VERSION     DAZAJZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.235"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..639
                     /locus_tag="ACG86D_002989"
     CDS             <1..639
                     /locus_tag="ACG86D_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN3142042.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSK
                     EEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQI
                     KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          243 a          121 c          114 g          184 t
ORIGIN      
        1 attgccattg gtatacgaga agacggcact aaagaagtac tgagttatgc gattgctcca
       61 actgaatcaa catacgtttg gaatgagctg ctacaggata ttaactccag aggagttcaa
      121 gaagtcttgc tttttattac ggacggctta aaaggcatga aagatactat ccatcaaatt
      181 tatcctaaag caaaatatca gcattgttgt atccatgtat ctcgtaacat cgctcataaa
      241 gtacgtgtca aagaccgaaa agaaatctgt gatgacttta aggctgttta tcaagctaac
      301 tcaaaagaag aagcgaatac cttcttatcc ggcatgattg agaaatggaa gaaaaactat
      361 cctaaagtga cgcagtcact catagaaaac caagacttat taacttttta tgattttcca
      421 cctagcattc gtagaaccat ttactcaacc aatctaatcg agtctttcaa taagcaaatt
      481 aaaagataca gccgtagaaa agagcagttt caaaatgaag aatcactaga acgctttcta
      541 gtcagcattt ttgatacata caatcaaaaa tttctaaaca gaagccataa aggttttcaa
      601 caggtaaccg atacattagt ttcaatgttt actgagtaac taattatttt gcaggaggac
      661 aa
//
LOCUS       DAZAJZ010000222          625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000222 DAZAJZ010000000
VERSION     DAZAJZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.236"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>625)
                     /locus_tag="ACG86D_002990"
     CDS             complement(109..>625)
                     /locus_tag="ACG86D_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFN3142043.1"
                     /translation="IVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKE
                     AFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYK
                     SVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDN
                     ERGSDTSGEAA"
BASE COUNT          183 a          144 c          115 g          183 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatg
//
LOCUS       DAZAJZ010000223          624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000223 DAZAJZ010000000
VERSION     DAZAJZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.237"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..436)
                     /locus_tag="ACG86D_002991"
     CDS             complement(<1..436)
                     /locus_tag="ACG86D_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3142044.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          184 a          112 c           87 g          241 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atcaaacctg ttat
//
LOCUS       DAZAJZ010000224          606 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000224 DAZAJZ010000000
VERSION     DAZAJZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.238"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..485
                     /locus_tag="ACG86D_002992"
                     /pseudo
     CDS             252..485
                     /locus_tag="ACG86D_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(533..606)
                     /locus_tag="ACG86D_002993"
                     /pseudo
     CDS             complement(533..606)
                     /locus_tag="ACG86D_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          232 a          116 c           93 g          165 t
ORIGIN      
        1 ttcataggag gattcaagat gtctcatact ctgcttatca aagataccct aaatattcta
       61 gacctaaata tcaatatcca tttcgaggaa aattgtttaa ctaaagaaaa atacaaagga
      121 caaatttgct tgatttacaa agggactctt cagtacacac cagaagaata tccacatctt
      181 ccaatatatt aaaagaacct tcttgaatca ttgagtgaaa gaaaagagat tcgtcactat
      241 ttgaggactg tttaactaga gacattagcg atctgcctga aacctataca accactttac
      301 gaacgtttaa aaattttctg ccacagattc aaaatgcgct taactgcagc tattcaaaca
      361 gaccattgga atgtttaaac aaccacatca aagtattaaa acgcaatacg tatggctttc
      421 gcagctttta caatttcaaa ctacggatca tgattcgtca tggcaaaaca tttttaacca
      481 aataaacaga agaatgtggg ataaatctaa aaatcagttt tgctacagac tctcgagtaa
      541 acggcgggag cagaagcaac tccttcggaa ataagccgaa attcacaaaa atttgaaaag
      601 caattt
//
LOCUS       DAZAJZ010000225          599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000225 DAZAJZ010000000
VERSION     DAZAJZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.239"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..599
                     /locus_tag="ACG86D_002994"
                     /pseudo
     CDS             1..599
                     /locus_tag="ACG86D_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          218 a          121 c          124 g          136 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata tttctcgtca gacctactat tatcaagcga aaccgatcga
      361 aaatgagtcc gacttagaag agatcgttca ggaagagttt attcgaaacc gaaaggctta
      421 cggtacccga aaattgaaga agtgtttagc aaagcgtggg cttcaactca gtcggcgccg
      481 aatcggtcga atcatgaaac gccgcggatt gacatctacc tatacgatcg ctcattttaa
      541 agggcaacga acagcttgta atgaagcgaa aacagcgaat gtattagatc ggaccttta
//
LOCUS       DAZAJZ010000226          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000226 DAZAJZ010000000
VERSION     DAZAJZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.240"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG86D_002995"
     tRNA            complement(65..150)
                     /locus_tag="ACG86D_002995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG86D_002996"
     tRNA            complement(167..238)
                     /locus_tag="ACG86D_002996"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG86D_002997"
     tRNA            complement(250..322)
                     /locus_tag="ACG86D_002997"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG86D_002998"
     tRNA            complement(340..421)
                     /locus_tag="ACG86D_002998"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG86D_002999"
     tRNA            complement(429..501)
                     /locus_tag="ACG86D_002999"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG86D_003000"
     tRNA            complement(505..577)
                     /locus_tag="ACG86D_003000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZAJZ010000227          585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000227 DAZAJZ010000000
VERSION     DAZAJZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.241"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..225
                     /locus_tag="ACG86D_003001"
     CDS             <1..225
                     /locus_tag="ACG86D_003001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142045.1"
                     /translation="VFRRKLIISKYSLKKLITIMPFLIYNTVIFKNKNAKSLYNNGES
                     TKNKKIITLLKKQMKDQVLLDLILSSLEGI"
BASE COUNT          202 a          106 c           63 g          214 t
ORIGIN      
        1 gttttcagac gaaaattaat aatttctaaa tattctttaa aaaagcttat aactattatg
       61 ccttttttaa tatataacac agttattttt aaaaataaga atgctaaatc actctacaat
      121 aacggcgaaa gcacaaaaaa taaaaaaatt attacgttat taaaaaaaca gatgaaagat
      181 caggttttat tagatctcat tctttcatca cttgaaggga tttaatgaac tactttttcc
      241 tctttgtcgt ttttatatat tcgtattcct tcaaacagta ggatagcctc tttcaatgct
      301 tgatatttga actgtccgtt acttaaaaat ttagatgtgc cgccctcttc ctagaacata
      361 ttctttttta ctttttcatt aattgacaga ccaatcgaac gaaactgtaa atttcgatag
      421 gataatgatt ggtcaaactc attcattttc aagacatctc gattcatatt caagatagga
      481 atctttttat caacatcact ttattcgtat tttcctctac cataaaagct tcacgctcca
      541 caaaattgtt ttatacacta ctattcagcc cattcaacga ccaat
//
LOCUS       DAZAJZ010000228          555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000228 DAZAJZ010000000
VERSION     DAZAJZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.242"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG86D_003002"
     CDS             131..505
                     /locus_tag="ACG86D_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3142046.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
BASE COUNT          196 a           74 c           97 g          188 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaa
//
LOCUS       DAZAJZ010000229          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000229 DAZAJZ010000000
VERSION     DAZAJZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.243"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG86D_003003"
     CDS             <1..>534
                     /locus_tag="ACG86D_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN3142047.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZAJZ010000230          506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000230 DAZAJZ010000000
VERSION     DAZAJZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.244"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..152)
                     /locus_tag="ACG86D_003004"
                     /pseudo
     CDS             complement(39..152)
                     /locus_tag="ACG86D_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          166 a           79 c           92 g          169 t
ORIGIN      
        1 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
       61 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
      121 atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa
      181 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag
      241 attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt
      301 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
      361 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
      421 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
      481 tagatccttt attctatgct ttatat
//
LOCUS       DAZAJZ010000231          497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000231 DAZAJZ010000000
VERSION     DAZAJZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.245"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..451)
                     /locus_tag="ACG86D_003005"
     CDS             complement(<1..451)
                     /locus_tag="ACG86D_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3142048.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          142 a           94 c           62 g          199 t
ORIGIN      
        1 aatttgtaat aagttcgtat atgttttgcg gtgtttcttg tttcttgtaa ttttccacga
       61 atgtttcatc catgaataaa ttcataaacg cttgaagtaa tcctacttgt tcttttggtt
      121 ctttgattcc taaaaaaaat agattttcta cagtcatttc ctgattatcc cccatttgat
      181 aaaaataaat tgggttcttc agctgcataa caaaaacaaa tggcttttcg acatattcac
      241 attccgaatg tggcagcgcg atatttaaat acttagtaat caatccagta ggaaattctc
      301 tttctcttgc aatcagtcca ttcaaataat tctcattgac gtaccccatt ttttctaatt
      361 gttgtgcagc gattaaaaaa gcttcttctt tattttttac ttctaccgct aattgaatca
      421 aactaggaac gaacatctct ttatatttca ttccaacctc ctcctttttg attaagtttg
      481 ttaatttatt gttctac
//
LOCUS       DAZAJZ010000232          488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000232 DAZAJZ010000000
VERSION     DAZAJZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.246"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG86D_003006"
     CDS             complement(<1..132)
                     /locus_tag="ACG86D_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN3142049.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          173 a           91 c           90 g          134 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaa
//
LOCUS       DAZAJZ010000233          487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000233 DAZAJZ010000000
VERSION     DAZAJZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.247"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACG86D_003007"
     CDS             complement(<1..>487)
                     /locus_tag="ACG86D_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3142050.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAZAJZ010000234          481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000234 DAZAJZ010000000
VERSION     DAZAJZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.248"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..>481
                     /locus_tag="ACG86D_003008"
     CDS             46..>481
                     /locus_tag="ACG86D_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN3142051.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          179 a           60 c           88 g          154 t
ORIGIN      
        1 ttatatcatg caaacgaagt attgaaaata gctgaggaga aataaatgga aattttattt
       61 actatttgtg gtagagctgg gtctaagggg tttaaaaata aaaatttgaa atatttggca
      121 ggaaagcccc tagtccattt cgctttagca atgatagata tctatcaaca aaaacatcct
      181 gaacataatt gcacgacttg tttgaatacg gacagtaatg agttagtaga attgacgact
      241 aagttaaatc cgtctgtgaa aattgtagaa cgtaaacctg aattagctac agatactgta
      301 gggaaaattg aagtgatacg tgattcattc ttacaactag aaagagaaga taatatattt
      361 gatttaatta tagatttaga tttgacttca cctttacgtg aagtttctaa tcttgaagaa
      421 ttaatagatg aacatctaaa aaatttagat aaagacgtct acttttcagt tgtttctgca
      481 a
//
LOCUS       DAZAJZ010000235          477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000235 DAZAJZ010000000
VERSION     DAZAJZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.249"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>477
                     /locus_tag="ACG86D_003009"
     CDS             <1..>477
                     /locus_tag="ACG86D_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142052.1"
                     /translation="LIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEF
                     WNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLL
                     GNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          175 a           59 c          103 g          140 t
ORIGIN      
        1 attaatcaaa gcagatatct ttcaaaattc ttttaacaag actggagatg tattgaaatt
       61 agttaatgga ctactccaaa tttggaacaa caaaaagaaa ataatggagt taactaaaaa
      121 aggaatggaa ttttggaatt ctaagggttc aattggaacg attggaacaa ctgattctgt
      181 tggtaatcct tttcctgggg cttctactcc aacccctatt gaagataatt ctttagttat
      241 tcgtacaaat ggagacggca aatatatttt gatttctcct aaagtaggaa aaggattagt
      301 tttattagga aatggtaaag caatttattt tggagactta gatgtacaag gaaaacttac
      361 agttaaagga aaagaaataa ttcctggaca aggtggtggt ccatcgggag gtggagaatc
      421 tactggtggg tatcctagtg aattaaaaac agatgcagaa aagagagctt ggagaat
//
LOCUS       DAZAJZ010000236          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000236 DAZAJZ010000000
VERSION     DAZAJZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.250"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG86D_003010"
     CDS             <1..>468
                     /locus_tag="ACG86D_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN3142053.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZAJZ010000237          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000237 DAZAJZ010000000
VERSION     DAZAJZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.251"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG86D_003011"
     CDS             complement(<1..430)
                     /locus_tag="ACG86D_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3142054.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZAJZ010000238          453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000238 DAZAJZ010000000
VERSION     DAZAJZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.252"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>453
                     /locus_tag="ACG86D_003012"
     CDS             <1..>453
                     /locus_tag="ACG86D_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN3142055.1"
                     /translation="IESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPIT
                     ADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWK
                     EKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          183 a           67 c          105 g           98 t
ORIGIN      
        1 tattgaaagt ggaacaacag tctttgcagt gaaagacggc gtaagcttac cagaagataa
       61 acttccagtg ttgaaagcaa aagatggtta tacagatgcg aaatggccag aggaagcaac
      121 tcaaccaatt acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgataa
      181 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
      241 accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      301 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      361 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      421 tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DAZAJZ010000239          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000239 DAZAJZ010000000
VERSION     DAZAJZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.253"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACG86D_003013"
     CDS             <1..>451
                     /locus_tag="ACG86D_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3142056.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAZAJZ010000240          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000240 DAZAJZ010000000
VERSION     DAZAJZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.254"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG86D_003014"
     CDS             complement(<1..339)
                     /locus_tag="ACG86D_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3142057.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZAJZ010000241          388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000241 DAZAJZ010000000
VERSION     DAZAJZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.257"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          135 a           75 c           36 g          142 t
ORIGIN      
        1 cctcttctgt ccaaattatt ctaagatttc tctttaccaa catcatttga caaagagctt
       61 cttttttgaa tactatattc gttctgtttt tcatcttcaa atacgaactc atccgcagaa
      121 atatttgtca caactccatg aatcttcttt tcattaccac ctatatacca caggattcct
      181 acacagaaac aacaaaaaaa tagcataaaa taggttttct ttattttata tccctccttt
      241 tagattaaaa gctcacagaa aacgctttct aaaaagacaa tagaataatc ataatttttc
      301 attttgtatt atttatacaa ataatacgca tgttactgaa aaatgatatg gttccctact
      361 ttttgagata agttaaataa aatctgtc
//
LOCUS       DAZAJZ010000242          354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000242 DAZAJZ010000000
VERSION     DAZAJZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.260"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>354
                     /locus_tag="ACG86D_003015"
     CDS             <1..>354
                     /locus_tag="ACG86D_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN3142058.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEP"
BASE COUNT          119 a           55 c           74 g          106 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaa
//
LOCUS       DAZAJZ010000243          338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000243 DAZAJZ010000000
VERSION     DAZAJZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.261"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>338
                     /locus_tag="ACG86D_003016"
     CDS             <1..>338
                     /locus_tag="ACG86D_003016"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3142059.1"
                     /translation="KLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLP
                     MVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQEL
                     KSYDVSYTTE"
BASE COUNT          139 a           44 c           64 g           91 t
ORIGIN      
        1 aaactgaaag ccaaagcagt agaattggat atagagaaaa gagtaaaatt tttaggagaa
       61 agaaacgata ttcccgagtt attatctgtg atggatgttt ttgtaatgcc ttctctttat
      121 gaaggtctgc caatggttgg attagaagca caagcagcaa aattaaaatt agtcttagca
      181 gatactattt ccaaagatac aaagttaaca aataatgtgg attttgtatc attagatgaa
      241 acaccacaag aatgggcaaa aaaagtaata aaagagccgt atgctaataa aataacacaa
      301 gaactaaaga gttatgatgt ttcgtacact actgaaca
//
LOCUS       DAZAJZ010000244          337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000244 DAZAJZ010000000
VERSION     DAZAJZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.262"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           50 c           63 g          111 t
ORIGIN      
        1 ccaacacaat ttgacgaaga gccactcttt tgggaatcag aggaaagaat caggctttat
       61 agagatattt catctaacaa attactttag actgacagta tatatcagca ggtactgtca
      121 tcgggaacgt agccaatatc gtatccaaaa cagcaggtag agaagctatt tgaggattca
      181 agttattcta tttcaaccat gaagttaagt tcattaaaaa taaagatagt tggtatttca
      241 ataatttttg tatagagtta atcactttat tgatgctaat tcatcttgat tggtattata
      301 atcgtattca agttgcgtgg cgtgcttttg ctagaaa
//
LOCUS       DAZAJZ010000245          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000245 DAZAJZ010000000
VERSION     DAZAJZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.264"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG86D_003017"
     CDS             complement(<1..>321)
                     /locus_tag="ACG86D_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3142060.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZAJZ010000246          307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000246 DAZAJZ010000000
VERSION     DAZAJZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.266"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG86D_003018"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG86D_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN3142061.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAZAJZ010000247          292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000247 DAZAJZ010000000
VERSION     DAZAJZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.268"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          118 a           47 c           34 g           93 t
ORIGIN      
        1 attgaaacat ccatctggaa tttgtctttt tcgttgcttt gctcataaaa atctgcatat
       61 tcatcactaa tattttttct aataaattct tccatcataa agatcacctc gaaaagagta
      121 tacgaacaaa cgttcttttt gtaaaggtgg aatttattat actaaataaa taggtgaaca
      181 agtacttgtg ccaaattatg tgccaaaaaa aatcgaattt aataaaacac aaacaaaaag
      241 aaatcctatt atgataacat ttcttataat aacaaacaca acagaagacg tg
//
LOCUS       DAZAJZ010000248          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000248 DAZAJZ010000000
VERSION     DAZAJZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.269"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG86D_003019"
     CDS             <1..>282
                     /locus_tag="ACG86D_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN3142062.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZAJZ010000249          268 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000249 DAZAJZ010000000
VERSION     DAZAJZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.270"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>268)
                     /locus_tag="ACG86D_003020"
     CDS             complement(36..>268)
                     /locus_tag="ACG86D_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3142063.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           79 a           39 c           47 g          103 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaata
//
LOCUS       DAZAJZ010000250          266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000250 DAZAJZ010000000
VERSION     DAZAJZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.271"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           28 c           49 g           77 t
ORIGIN      
        1 aggtgtcact aaaaattata aaaaatagtg ttgacgcact tttgatgtca tgttataata
       61 acgaagtcgt ttggatgaga tagctaataa agcttgattt tatcgaaaaa aacttcgaaa
      121 aagttattga caaataacaa taaagatggt atgataatca agtcgctgct aatgagtggc
      181 aaagacgttt agaaaaaata aacaaagaag ttgttgacat tctcttctga atctgataag
      241 attaaagagt tgttaaaaca aaaaga
//
LOCUS       DAZAJZ010000251          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000251 DAZAJZ010000000
VERSION     DAZAJZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.272"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG86D_003021"
     CDS             <1..>253
                     /locus_tag="ACG86D_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN3142064.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZAJZ010000252          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000252 DAZAJZ010000000
VERSION     DAZAJZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.274"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>233
                     /locus_tag="ACG86D_003022"
     CDS             22..>233
                     /locus_tag="ACG86D_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3142065.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEI"
BASE COUNT           87 a           44 c           44 g           58 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat cat
//
LOCUS       DAZAJZ010000253          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000253 DAZAJZ010000000
VERSION     DAZAJZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.276"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZAJZ010000254          228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000254 DAZAJZ010000000
VERSION     DAZAJZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.277"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>228
                     /locus_tag="ACG86D_003023"
     CDS             <1..>228
                     /locus_tag="ACG86D_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3142066.1"
                     /translation="LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSI
                     SNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           82 a           42 c           41 g           63 t
ORIGIN      
        1 cttgaaacca gaaggccgta aggcagttga tattgaaaat tcgattaatg aatggcttca
       61 atccgtaccc aaaaatcttt tcaaatcaat tacctttgat tgtggaaaag aattctctaa
      121 ttggaaaagt attagtaata cgaatgatat tgatatttat ttcgcagacc caggaacgcc
      181 ttcccaacgg ggattaaatg aacattcaaa cggactcctc cgaaaaga
//
LOCUS       DAZAJZ010000255          227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000255 DAZAJZ010000000
VERSION     DAZAJZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.278"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACG86D_003024"
     rRNA            complement(<1..>227)
                     /locus_tag="ACG86D_003024"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAZAJZ010000256          224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000256 DAZAJZ010000000
VERSION     DAZAJZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.279"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG86D_003025"
     CDS             <1..>224
                     /locus_tag="ACG86D_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN3142067.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZAJZ010000257          220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000257 DAZAJZ010000000
VERSION     DAZAJZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.280"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG86D_003026"
     rRNA            complement(<1..175)
                     /locus_tag="ACG86D_003026"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAZAJZ010000258          205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000258 DAZAJZ010000000
VERSION     DAZAJZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.denovo.281"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>205
                     /locus_tag="ACG86D_003027"
     CDS             <1..>205
                     /locus_tag="ACG86D_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3142068.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DAZAJZ010000259        13087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.079, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000259 DAZAJZ010000000
VERSION     DAZAJZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.079"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2036)
                     /gene="nagE"
                     /locus_tag="ACG86D_003028"
     CDS             complement(78..2036)
                     /gene="nagE"
                     /locus_tag="ACG86D_003028"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN3142069.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2033..2905)
                     /locus_tag="ACG86D_003029"
     CDS             complement(2033..2905)
                     /locus_tag="ACG86D_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3142070.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3247..4119)
                     /locus_tag="ACG86D_003030"
     CDS             complement(3247..4119)
                     /locus_tag="ACG86D_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3142071.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4247..4816
                     /locus_tag="ACG86D_003031"
     CDS             4247..4816
                     /locus_tag="ACG86D_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN3142072.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            4883..5620
                     /locus_tag="ACG86D_003032"
     CDS             4883..5620
                     /locus_tag="ACG86D_003032"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3142073.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5851..7119
                     /locus_tag="ACG86D_003033"
     CDS             5851..7119
                     /locus_tag="ACG86D_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3142074.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7606..8070
                     /locus_tag="ACG86D_003034"
     CDS             7606..8070
                     /locus_tag="ACG86D_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3142075.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8054..8683
                     /locus_tag="ACG86D_003035"
     CDS             8054..8683
                     /locus_tag="ACG86D_003035"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3142076.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9037..10515
                     /gene="msr(C)"
                     /locus_tag="ACG86D_003036"
     CDS             9037..10515
                     /gene="msr(C)"
                     /locus_tag="ACG86D_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN3142077.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10562..11008
                     /locus_tag="ACG86D_003037"
     CDS             10562..11008
                     /locus_tag="ACG86D_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN3142078.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11207..11722)
                     /locus_tag="ACG86D_003038"
     CDS             complement(11207..11722)
                     /locus_tag="ACG86D_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3142079.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11839..13041
                     /locus_tag="ACG86D_003039"
     CDS             11839..13041
                     /locus_tag="ACG86D_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3142080.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4076 a         2405 c         2678 g         3928 t
ORIGIN      
        1 gaaaaaggag ctgagacaag atttttgtct cagctcctgt ttgtttatcg ataaaaaagt
       61 ctggggaaga cgatggctca ggctttgttg gaaacagagc cgattttatc atttgctttt
      121 acatcacgtg cgggttcgat ttctaagctt tggactttgt cgccatttgt gaatacaacg
      181 accatatccg tgtttttgcc tgcagattga atagcggcaa gatcaactaa agcaatcaat
      241 tgacctcttg cgactttttg accttctttt acatataaag tgaatggttc gccttttaag
      301 tcaacagtgt tgatgcccat atgaagcaag acttcgatac cagcatctgt ttggattccg
      361 acagcatgtt tcgttgggaa aatgtttgtg atcgtaccag cgattggaga gaagatttct
      421 ccattttctg gtaatacagc atagccgtca cccatcattt tttccgcgaa tacatcatct
      481 gaaacttcag tgattggaat cagtttccca tttgcgatag cgtaaatatc ttgttcgatc
      541 gttccaccgg caaatgtttc tgttttaacc acggtgtttg tttgtgcagg tcgtgttgca
      601 gcaccaccat gtagttttgc catctcatct gcaacgaatt gtacttctgt acctacgatg
      661 acttgcgcat tttttccgtc gatgatcttc ataccaggta cgccagtggc tttgatttta
      721 ttttgatcga tcaggcctgt atctttgact tgcaaacgaa ggcgtgtagt acagttatcg
      781 atcgaagtta cattgtcggc tccgcctaat gcagcgtaga ttttgcttgc ttgtgccgca
      841 tatttattgt ctgaattagt cgttgcagta tcgtcatctg aaagatctgc ttcctcgcgg
      901 ccaggagtca tcagatggaa ttttttgata gcaaagttaa agccaaagta gtaaatcact
      961 gctgttacta atccttggac gatcaacatc aatggctgat tcgcaatcgg atttttcaaa
     1021 gataggaagt aatcgacaaa tccggcacta aaggcaaatc ctgctgtcca atggaagaat
     1081 gcactcactg ctaaagaaat cccagtgaaa attgcatgca agacatataa tggccaagca
     1141 acgaacataa atgagaattc aagtggttct gttacaccag taaagaatga agcaaaagct
     1201 gctgctaaca tcaaggaagc tgtttttgct ttgttttctg gacgtgcatt tctgtaaata
     1261 gcatatgctc ctgctggaag cccgaacatc atgattggga aaaatccagc ttgatacatt
     1321 cctgttacgc cttttgttcc ttcagaagcc aaaaatttac caatatcatt gattcctgct
     1381 acgtcaaacc agaacacgga gttcattgct tgatgcaatc ctgtagggat cagtaaacgg
     1441 ttgaagaagc cataaagacc tgcaccaccc cagcctaaac tcaaaatgaa tttagcaaaa
     1501 gaaactaaag cagagtaaac cggaggccat acgagcagta gcactgcact gatcagcaac
     1561 atgacaaagg cagccaagat tggcactaga cgcttgccgc taaagaaaga taaagccatc
     1621 ggtaattttg cttcgtggaa acgattataa agagctgccg caatcaatcc ggcaataata
     1681 ccaataaata cgttactgat ctgagcaaaa gatggatcaa ctgattctgg tttgatattc
     1741 aataaagtag ctactgaatc agtttttaaa acatttactg gaacttcaaa tgcgaccaaa
     1801 cctgccaagg cagcagcacc tgatttgtcc actgacatcc ctagtgctaa cccaacggca
     1861 aacagcaaag gtaattgacc taaaactgcg ttaccgccag cttgtaaaaa atgagcgaca
     1921 atgtcgttac tataagatgc ccaccagttt ccgatcccta ctagtaaagc agcagcaggt
     1981 aagaccgcaa ctggaagttg aagggaacgt cccattcgtt gtagatatgc tttcaatttt
     2041 tttcctcctc atgattgtta acatttgttt gtacgattgc gcttcttaaa cgctcgatat
     2101 ggatggccaa gtaacccagt tcttctttcg tgatcgcgag ctggtattct ttggtcagca
     2161 acacacggat ttttttagaa atcttatagc tttctgggta ttttcgtttg accatttcta
     2221 cgatttccgt gtctaaaaca gcatattgtt gcgcatgggt tcgttgtaga agcagtcgta
     2281 aatgattcac cagtcttgaa taattcagtg aaaatggttc tgaatggata tctgtttcca
     2341 attctgattc cactaaatgg atgatgtcag atacaatcgt cacttcacgg atactgcgat
     2401 ggttattgtt ccgaccgctt cgtccgctgt gaatatggat tgcgatatat cctgcttcgt
     2461 cataagtata tggaatccca agctcttttg tcagatactc tactgcccat tgagcaatac
     2521 tgaattcatc actgtaaagc acttcgattt ctcttaaaag tttgttgcgg atgacgatac
     2581 cgttattaat gttctccgca gaaaaagcaa tgtggtcggt caaagcgatc aatacatgtt
     2641 cattcaactt ttccatcaaa acagtttccg catgatcaat gatcgtttct gctgcaaata
     2701 aaaagcgttc atcaatctga ttaagtaaat tttgcaactt gatttgacct tctggttcca
     2761 tgacaaatat ccgttcgaca tcacgatcaa aaatcaaatc atttcttttt ctgtcaaatc
     2821 caatcccttt tccaatagct actttttcct gtccaccgtc atctaccaat actgcatttt
     2881 gattaaagat ctttttgatt ttcatgcgcg cacccttttt gaagatttgg ttttcacgtt
     2941 tgctagacca gttgattgcg gccaaacaaa aatagacacg aaaaacctcc tagggaaacc
     3001 atgttcctca gagatttttc atgcctagta tgatctagtc acatgaaacg aattattgtt
     3061 aacgtaatca attttatata gacagttatg ttttgtcaat tcttttttta taattatagt
     3121 aatttactgg aattatcaga aataaaagga aggaacaaat taagaaactt atgttattta
     3181 atcattggat cttttgctta acttattcgt tgttaaaaag ctgaaagcga aggaaaaaaa
     3241 ctctacttaa ctgtatttct ccgttaggac atctaaaaat ttacgagcag caggagaaag
     3301 aggctggtct tttttccaaa tgagattcag tgaagcttgt aatggcggat cgagtgggat
     3361 gaatactttt tccgtcccat aagtattgat gatcccatct agagctaatg caccaaaagt
     3421 cccgttatcg accatcaaag aagcattgta caataagtta tatgttccga cgatcgataa
     3481 ctgatctatg ctgaaaccta gccattcagc tagttgactg tccactgacg tttgccggga
     3541 aatgatcaaa ggaacagttt tcagttcctg cggatggatg actgtttgct ttgctaaagg
     3601 atgattccga tgtatcaaaa gtccccaacg atcttgggct ggcagtcgaa atgactggta
     3661 tttttgtttg tttgtcggtt cgattacgag tccaaaatca agtaatccgc tatccagttt
     3721 gtctatgata tcatctgcat tcccgctata taaatgaaac tggatatcag gatgcgcctt
     3781 gatcaacgaa tgaaggctat cagaaatgaa agagattgct ttagtttcgc ctccgccaat
     3841 gtagatttct ccagagatga ttgcgtcatt tgttaaattg ttcgccgttt tttctaccaa
     3901 cgacaagatt tcttttcctt ttgaataaag atatcgacca gaatccgtca actgtatctc
     3961 tcgggagcct cgagtgaaaa gtgtcacacc aagttcttct tctaaatctt tcagttgttt
     4021 tgagagagta ggttgcgtga tatgcagaga ttttgctgct cggctgatat ttttttctct
     4081 agctactgct aaaaaatacg tcagtatacg aatatccatt ttttccctcc tgtttttaaa
     4141 aaagcatacc actaagtata gttaaaagga atagttaagt atgttttata agtattagac
     4201 atcataagcc gtccgttata aagtaaggat aaaaggaggg agcaaaatga caagcctcat
     4261 cgaaaaacaa atcgtcaata aagagattcg agtaacggat ccgctctttg aagtgatcca
     4321 tcagatccaa gcggaaaacg aagagccttt agcagcgctg aatactgggt accatgaacc
     4381 agcagatatc cgcaaacggc tagaaaatat cattagtgac aaagtagatg atacggtgac
     4441 tgttctgctg cctttttata cagatttcgg caaacatatt agtataggga aaaatgtctt
     4501 tatcaatcgt caagtgatgt tcgttgactt aggtggcatc tgtttagaag attcagtatt
     4561 gattggtcct cgtgtcaacc tcatcacggt gaaccatcta accgatccaa aagaacgaag
     4621 aggcttgtca gtaaagccaa tccacatcaa gaaaaatgct tggatcggag caggagcaac
     4681 tattctgcca ggcgtaacga ttggcgagaa tgccattgtt gcagcgaatg caactgttac
     4741 gaaagatgtc ccagacaata ccatcgttgc aggaatccct gccaaaatcg tcaaacctgt
     4801 cgaacggatt atttgaacag taagatgaaa taagagatag tgagtataag caagagattt
     4861 ttcaaaacag gaggagaata gaatgaatga tttatcagaa aaagtgattg tcatcatggg
     4921 cgcttcaagc ggaatcggtg aagcaacaac gaagctatta gcagaaaaag gggctaaact
     4981 agttatagct gcacgccgag aagatcgcct aaaagcaata aaagaatctt taccagaagc
     5041 tgagctatat attcaaacag cagatgtaag agattttgca caagtccaag cagttatcga
     5101 tttagcgatg gaaaagttcg gtcgaatcga tgtattgtac aataatgcag gaatcatgcc
     5161 aaccgctcca ttagttgaag gtcaccgtga tgaatggcaa aatatgcttg atatcaacat
     5221 catgggtgtg ttaaatggaa tttccgccgt acttccaatc atggaaaaac aaaaatctgg
     5281 gcatatcatt tcaactgatt ctgtggcggg acatgttgtt tatcctgatt ccgccgttta
     5341 ttgcggaaca aaatttgctg tccgtgcaat catggaagga ttacgtcaag agcaacggca
     5401 aaataacatc aaatctacga tcatctctcc aggagctgtt caaactgaat tatatcaaac
     5461 catcagcgac aaaaaggctg ctcttgaact tcatgaagcg caaaaagaat ggggattaac
     5521 atctgaagat atagcttctg ctgtagcatt tgcgatagaa acgccagacc ggatgtctgt
     5581 cagcaacatg attatccgtc cgacgacaca ggaagtataa tccataacgg ataatatcca
     5641 gagagaatta cttgttaaat agttattcaa acgaccatca atttaaggcg tgaatggaga
     5701 agaatattct tcatttacgt ctttttatgc agaaaaagca aatttattaa aaagaaacaa
     5761 atgttattta aatatggaca ttgacttgct tttacttttt tagttacact gttatttagg
     5821 aaaagtttca taattcaaag gagaaaaaag atgaaagcaa agaatttatt atttagcgtt
     5881 attcttttag gaaatctagc agttgccaac acaacagtgc tagctgaaga atcgacagaa
     5941 actgtcgcaa ctcagacatc gagttcttca gaaacaggaa ttgaaaacga tacagaaagt
     6001 actttgcagt cagctactgt ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa
     6061 acaacagaac aaacacaaac cgaagacagt acatcagcag aagctcagaa caatccagag
     6121 aatgcagaag aaatcacgaa ccaattgaca gatgggcaaa cattgacaca tacccatcaa
     6181 gcagatttgt ctgaattcga aaactatact ggctatgctt tttatgctcg ctcatctgct
     6241 aattctcaac aagcgttcat tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc
     6301 aatgacttat atgcatcagt catgattgct caagccatcg ttgaaagtgg ctgggggaac
     6361 agtactttgg cttctgcccc taattacaat ctatttggga ttaaaggaag ctataatggc
     6421 caatctgtta cgatgcctac ttcggaatat gtgaatgggc aatggattac ggtgaatgcc
     6481 gcttttagaa aatatccttc gtacaaagaa tcattgcaag ataacgtaac ggtattgaag
     6541 acgacttctt ttcagccagg cgtttactac tattccggtg catggaaaag caatacgaac
     6601 tcttacaaag atgctacggc ttggctaaca ggtcgttatg ccactgcgcc taattatggt
     6661 tctacattga acaatgtaat cgaaacctat aatctgacac agtatgatac agcaccaact
     6721 tcaacttctc cgatgtatcg tctgtataac cgtcatacag gagaacattt atatacattg
     6781 aatgccggag aaaaaaatta tctgccaact gtaggctggg aatatgaagg gattgcttgg
     6841 caagcaccaa acagcggaca acctgtctac cgtttataca atccttacag tggagaccat
     6901 cattacacaa tggcacaatc tgagatcaac tctttaacaa aaatcggttg gcgttatgaa
     6961 ggtctaagtt tttattctgg tggttccaag ccaatttatc gactatttaa tccaaatgaa
     7021 aaaactggta cccatcacta taccttgagt gctaaggaaa gagattggct aacaccgatg
     7081 ggctggagat atgaaggaat aggattttat ggttattaaa aagaggtagt gaaaaagctg
     7141 ccctgcatca agtagtaaga acagccaaac aaaaacagat tttcatgttt tttgtttggt
     7201 tgaacttatt accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca
     7261 acatcttgtc ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg
     7321 acgggattct tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt atctactgca
     7381 gaagagctat cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt
     7441 ctttatcatt ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga
     7501 gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta ttctattatt
     7561 gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag
     7621 attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa
     7681 gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat
     7741 ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt
     7801 catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac
     7861 cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcaagca ttgtgaaatg
     7921 gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa
     7981 tttctaacac ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa
     8041 gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc
     8101 cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa
     8161 agaagcgacg actgcacctt cttctgtcaa tatgaaacca tggcgctttg tggtcgctga
     8221 aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga
     8281 tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga
     8341 aatctacaac caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt
     8401 agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa
     8461 agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga
     8521 aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg gtttggataa
     8581 agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga
     8641 gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa taaaaggttt tcagttacta
     8701 ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga
     8761 aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca
     8821 aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt
     8881 agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta
     8941 ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat
     9001 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
     9061 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
     9121 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
     9181 aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc
     9241 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
     9301 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
     9361 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
     9421 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
     9481 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
     9541 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
     9601 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
     9661 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
     9721 gtgtccagta aaaagaaaaa gcaacaaatc agactggatc ggttgtcttc ctctaaacaa
     9781 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
     9841 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
     9901 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
     9961 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
    10021 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
    10081 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
    10141 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
    10201 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
    10261 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
    10321 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
    10381 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
    10441 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
    10501 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
    10561 attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt
    10621 cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt
    10681 gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt
    10741 gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat
    10801 cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt
    10861 gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat
    10921 gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt
    10981 gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc
    11041 atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg
    11101 ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga
    11161 taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact
    11221 gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc
    11281 tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca
    11341 attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc
    11401 cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt
    11461 tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat
    11521 cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac
    11581 cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa
    11641 ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata
    11701 atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc
    11761 acagtaaagt ttccacgttt tacatgtgca tattgacatg taaaataaat catgtatgat
    11821 gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca
    11881 aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct
    11941 tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct
    12001 acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg
    12061 ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aaacattgat gacaggattg
    12121 tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt
    12181 gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg
    12241 atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt
    12301 ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt
    12361 tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt
    12421 ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta
    12481 agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt
    12541 actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt
    12601 gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt
    12661 gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt
    12721 ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta
    12781 gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca
    12841 ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct
    12901 ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat
    12961 tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt
    13021 gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc
    13081 ataagaa
//
LOCUS       DAZAJZ010000260        10038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.100, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000260 DAZAJZ010000000
VERSION     DAZAJZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.100"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..903
                     /locus_tag="ACG86D_003040"
     CDS             7..903
                     /locus_tag="ACG86D_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN3142081.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1053..1994)
                     /locus_tag="ACG86D_003041"
     CDS             complement(1053..1994)
                     /locus_tag="ACG86D_003041"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3142082.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2120..3241
                     /locus_tag="ACG86D_003042"
     CDS             2120..3241
                     /locus_tag="ACG86D_003042"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3142083.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3499..5001
                     /gene="eat(A)"
                     /locus_tag="ACG86D_003043"
     CDS             3499..5001
                     /gene="eat(A)"
                     /locus_tag="ACG86D_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN3142084.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5068..5595
                     /locus_tag="ACG86D_003044"
     CDS             5068..5595
                     /locus_tag="ACG86D_003044"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3142085.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5806..6867
                     /locus_tag="ACG86D_003045"
     CDS             5806..6867
                     /locus_tag="ACG86D_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN3142086.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6870..7766
                     /gene="murQ"
                     /locus_tag="ACG86D_003046"
     CDS             6870..7766
                     /gene="murQ"
                     /locus_tag="ACG86D_003046"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN3142087.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7780..9762
                     /locus_tag="ACG86D_003047"
     CDS             7780..9762
                     /locus_tag="ACG86D_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN3142088.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9827..>10038)
                     /locus_tag="ACG86D_003048"
     CDS             complement(9827..>10038)
                     /locus_tag="ACG86D_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN3142089.1"
                     /translation="MREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPF
                     LADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3285 a         1783 c         2191 g         2779 t
ORIGIN      
        1 gaaaagatga acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg
       61 ccttccatcc gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt
      121 aaatcagcta gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt
      181 gatatggaga tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt
      241 tggaatcact tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca
      301 atcgatgaga tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat
      361 agttcatttt attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt
      421 tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata
      481 aaaagagatg ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga
      541 tatcttactt atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg
      601 ttgcgggagg gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag
      661 aaactagtga cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt
      721 tttttgcttc gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag
      781 ccaatcctat cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc
      841 actaacgatt ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa
      901 taaaaaacct acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc
      961 atcatgagag ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct
     1021 gctgattcag ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt
     1081 gcatggacta attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct
     1141 tttcgccaaa tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg
     1201 tcttggtaca tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata
     1261 tgttggtaca cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat
     1321 tcagatacga ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata
     1381 ttacaatcca aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa
     1441 atggaataac gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct
     1501 acaacttttt ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca
     1561 tcacctaaca atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca
     1621 aataagatat ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt
     1681 agtaaacaca aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca
     1741 tctaattgat tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg
     1801 atttcttctg gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca
     1861 atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc
     1921 tcatttccgc tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct
     1981 attgcgattt tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa
     2041 aaatggaaga aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag
     2101 aagaaagtga gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc
     2161 ttcttcattt gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa
     2221 aatcaatgtg ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct
     2281 caggggaaca tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag
     2341 ctatttattg acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt
     2401 ggggttagat ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc
     2461 cttaggagaa cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt
     2521 aggaggaagc gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt
     2581 agaagggtac acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag
     2641 aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac
     2701 aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct
     2761 agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt
     2821 ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt
     2881 gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa
     2941 aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat
     3001 ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat
     3061 cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt
     3121 cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt
     3181 agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta
     3241 gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat
     3301 ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag
     3361 acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt
     3421 ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt
     3481 tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac
     3541 agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta
     3601 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
     3661 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
     3721 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
     3781 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
     3841 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
     3901 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
     3961 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
     4021 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
     4081 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
     4141 caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
     4201 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
     4261 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
     4321 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
     4381 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
     4441 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
     4501 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     4561 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     4621 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     4681 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     4741 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     4801 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     4861 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     4921 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     4981 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     5041 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     5101 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     5161 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     5221 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     5281 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     5341 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     5401 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     5461 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     5521 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     5581 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     5641 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     5701 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     5761 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     5821 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     5881 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     5941 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     6001 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     6061 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     6121 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     6181 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     6241 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     6301 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     6361 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     6421 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     6481 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     6541 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     6601 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     6661 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     6721 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     6781 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat
     6841 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     6901 cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc
     6961 aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta
     7021 tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata
     7081 tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat
     7141 tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg
     7201 tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga
     7261 atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     7321 tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata
     7381 aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat
     7441 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga
     7501 tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca
     7561 aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag
     7621 ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta
     7681 tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa
     7741 acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact
     7801 ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc
     7861 attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc
     7921 tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc
     7981 atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg
     8041 aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga
     8101 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     8161 ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct
     8221 gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     8281 gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     8341 aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt
     8401 gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg
     8461 gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc
     8521 ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta
     8581 gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg
     8641 attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg
     8701 atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc
     8761 ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt
     8821 gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg
     8881 ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc
     8941 aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca
     9001 ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg
     9061 atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc
     9121 ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct
     9181 ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa
     9241 ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca
     9301 gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa
     9361 gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa
     9421 aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac
     9481 gctattggga tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca
     9541 gttgacttag gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct
     9601 gatacgcttg tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc
     9661 atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt
     9721 aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga
     9781 aacgaaagaa gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt
     9841 ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg
     9901 ccaagaaagg aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat
     9961 tgaagttttt atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt
    10021 ccagatattc acgcatcg
//
LOCUS       DAZAJZ010000261        10038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.101, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000261 DAZAJZ010000000
VERSION     DAZAJZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.101"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..903
                     /locus_tag="ACG86D_003049"
     CDS             7..903
                     /locus_tag="ACG86D_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN3142090.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1053..1994)
                     /locus_tag="ACG86D_003050"
     CDS             complement(1053..1994)
                     /locus_tag="ACG86D_003050"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3142091.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2120..3241
                     /locus_tag="ACG86D_003051"
     CDS             2120..3241
                     /locus_tag="ACG86D_003051"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3142092.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3499..5001
                     /gene="eat(A)"
                     /locus_tag="ACG86D_003052"
     CDS             3499..5001
                     /gene="eat(A)"
                     /locus_tag="ACG86D_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN3142093.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     FKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5068..5595
                     /locus_tag="ACG86D_003053"
     CDS             5068..5595
                     /locus_tag="ACG86D_003053"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3142094.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5806..6867
                     /locus_tag="ACG86D_003054"
     CDS             5806..6867
                     /locus_tag="ACG86D_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN3142095.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6870..7766
                     /gene="murQ"
                     /locus_tag="ACG86D_003055"
     CDS             6870..7766
                     /gene="murQ"
                     /locus_tag="ACG86D_003055"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN3142096.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7780..9762
                     /locus_tag="ACG86D_003056"
     CDS             7780..9762
                     /locus_tag="ACG86D_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN3142097.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9827..>10038)
                     /locus_tag="ACG86D_003057"
     CDS             complement(9827..>10038)
                     /locus_tag="ACG86D_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN3142098.1"
                     /translation="MREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPF
                     LADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3285 a         1783 c         2190 g         2780 t
ORIGIN      
        1 gaaaagatga acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg
       61 ccttccatcc gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt
      121 aaatcagcta gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt
      181 gatatggaga tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt
      241 tggaatcact tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca
      301 atcgatgaga tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat
      361 agttcatttt attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt
      421 tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata
      481 aaaagagatg ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga
      541 tatcttactt atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg
      601 ttgcgggagg gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag
      661 aaactagtga cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt
      721 tttttgcttc gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag
      781 ccaatcctat cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc
      841 actaacgatt ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa
      901 taaaaaacct acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc
      961 atcatgagag ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct
     1021 gctgattcag ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt
     1081 gcatggacta attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct
     1141 tttcgccaaa tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg
     1201 tcttggtaca tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata
     1261 tgttggtaca cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat
     1321 tcagatacga ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata
     1381 ttacaatcca aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa
     1441 atggaataac gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct
     1501 acaacttttt ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca
     1561 tcacctaaca atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca
     1621 aataagatat ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt
     1681 agtaaacaca aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca
     1741 tctaattgat tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg
     1801 atttcttctg gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca
     1861 atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc
     1921 tcatttccgc tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct
     1981 attgcgattt tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa
     2041 aaatggaaga aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag
     2101 aagaaagtga gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc
     2161 ttcttcattt gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa
     2221 aatcaatgtg ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct
     2281 caggggaaca tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag
     2341 ctatttattg acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt
     2401 ggggttagat ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc
     2461 cttaggagaa cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt
     2521 aggaggaagc gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt
     2581 agaagggtac acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag
     2641 aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac
     2701 aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct
     2761 agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt
     2821 ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt
     2881 gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa
     2941 aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat
     3001 ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat
     3061 cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt
     3121 cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt
     3181 agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta
     3241 gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat
     3301 ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag
     3361 acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt
     3421 ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt
     3481 tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac
     3541 agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta
     3601 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
     3661 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
     3721 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
     3781 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
     3841 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
     3901 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
     3961 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
     4021 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
     4081 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
     4141 caaaatgaaa aatttaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
     4201 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
     4261 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
     4321 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
     4381 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
     4441 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
     4501 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     4561 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     4621 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     4681 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     4741 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     4801 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     4861 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     4921 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     4981 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     5041 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     5101 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     5161 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     5221 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     5281 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     5341 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     5401 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     5461 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     5521 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     5581 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     5641 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     5701 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     5761 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     5821 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     5881 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     5941 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     6001 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     6061 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     6121 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     6181 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     6241 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     6301 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     6361 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     6421 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     6481 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     6541 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     6601 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     6661 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     6721 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     6781 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat
     6841 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     6901 cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc
     6961 aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta
     7021 tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata
     7081 tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat
     7141 tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg
     7201 tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga
     7261 atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     7321 tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata
     7381 aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat
     7441 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga
     7501 tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca
     7561 aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag
     7621 ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta
     7681 tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa
     7741 acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact
     7801 ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc
     7861 attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc
     7921 tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc
     7981 atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg
     8041 aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga
     8101 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     8161 ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct
     8221 gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     8281 gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     8341 aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt
     8401 gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg
     8461 gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc
     8521 ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta
     8581 gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg
     8641 attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg
     8701 atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc
     8761 ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt
     8821 gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg
     8881 ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc
     8941 aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca
     9001 ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg
     9061 atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc
     9121 ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct
     9181 ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa
     9241 ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca
     9301 gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa
     9361 gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa
     9421 aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac
     9481 gctattggga tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca
     9541 gttgacttag gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct
     9601 gatacgcttg tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc
     9661 atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt
     9721 aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga
     9781 aacgaaagaa gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt
     9841 ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg
     9901 ccaagaaagg aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat
     9961 tgaagttttt atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt
    10021 ccagatattc acgcatcg
//
LOCUS       DAZAJZ010000262         6943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.118, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000262 DAZAJZ010000000
VERSION     DAZAJZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.118"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(250..486)
                     /locus_tag="ACG86D_003058"
     CDS             complement(250..486)
                     /locus_tag="ACG86D_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3142099.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(669..1040)
                     /locus_tag="ACG86D_003059"
     CDS             complement(669..1040)
                     /locus_tag="ACG86D_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN3142100.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1037..2365)
                     /locus_tag="ACG86D_003060"
     CDS             complement(1037..2365)
                     /locus_tag="ACG86D_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN3142101.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2326..2655)
                     /gene="mobC"
                     /locus_tag="ACG86D_003061"
     CDS             complement(2326..2655)
                     /gene="mobC"
                     /locus_tag="ACG86D_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFN3142102.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2913..3283)
                     /locus_tag="ACG86D_003062"
                     /pseudo
     CDS             complement(2913..3283)
                     /locus_tag="ACG86D_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3428..3613
                     /locus_tag="ACG86D_003063"
     CDS             3428..3613
                     /locus_tag="ACG86D_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN3142103.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3771..3938
                     /locus_tag="ACG86D_003064"
     CDS             3771..3938
                     /locus_tag="ACG86D_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142104.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4556..5215
                     /gene="vanR-B"
                     /locus_tag="ACG86D_003065"
     CDS             4556..5215
                     /gene="vanR-B"
                     /locus_tag="ACG86D_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN3142105.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5218..6579
                     /gene="vanS-B"
                     /locus_tag="ACG86D_003066"
     CDS             5218..6579
                     /gene="vanS-B"
                     /locus_tag="ACG86D_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN3142106.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6670..>6943)
                     /locus_tag="ACG86D_003067"
     CDS             complement(6670..>6943)
                     /locus_tag="ACG86D_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3142107.1"
                     /translation="DKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPY
                     SNAKIENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1734 a         1611 c         1716 g         1882 t
ORIGIN      
        1 tcttttcttt ttgcctattc ctacgcattt agaacccggt attgtcgggg ataggatgaa
       61 gtattcgctt gcccctgcaa acatgcggga gaagccttgc agcgggcggt tttcaatccc
      121 ttaaatgcgt aggttgcagc tataattaga cccctgaaac agatatagcc gcgcagcggg
      181 tgtatctgtt tcaggggtgt gcaggtgata gccgcaaagc ggcgtaagag ggtgcagccc
      241 tcttgccgtc tatctctgtt ccagcgggaa gctcggcaat gcttccagca gtttcaggga
      301 aatgcgctgc cgtatatctt cgtcaatgac tttcttcttt ttgccgtttg cctgtctgat
      361 ctccaccgtg gaaagctcgt tgatgtagtc agcataatgg gccaacactt tttccgtggc
      421 ccacctctca ccggcaacgg cggctttgat cgtttcatag tccagcagct tttcttgtcc
      481 gtccatcctg atacccccgt cagactgatt ttttctatga taccatattc ggaagcatgg
      541 aaccagagaa aacggcgctt ttctgctggt aaattctacg catttagaac agcacttcgg
      601 cggggatagg ataaaacacc cacatacccc gctgaccatg ccggaaaagc cttgaaatat
      661 aagggctttt acagctctaa gtgcgtaggt ttccagtatc ttttccgctg gcgctcggcc
      721 tctttgcggc ggcgcatccg tttggagcag gccgcgcagt atttggcccg gttagacttc
      781 ggcgtgaacg tggccccgca ctcacagcag cgtttcactt cgtcccggtc tttggtgatc
      841 tcggcataca gggccgcatc cagcgggagg acagcggcct taaaccagcg gcacagaagc
      901 gaataggaaa tgctctgtac gcaaatacat tcttcgccgt cgtccagcaa caggcagttg
      961 ccgtcacaat agttgcagca ctcatgcgtc agcttccggg ccttgcgata ttgagggtaa
     1021 ttcattcgtg gaatactcat agctcttgcg tccattcctt tcccggcgtc atacgcaaaa
     1081 tgccgtccac attctgcttg atgatgccgt attcctgcat ctgctttttc gcttcgccgt
     1141 acttctcata cagccgttcc ttttcctcgg ccaatcgctt gtactcggct ttcagggcgt
     1201 acagatcggg gagctttttc acaccctgtc cctttagggc ctttgccgca gcttcataca
     1261 aaatcaacgt gctttcatgc tcacgccgga attttgcctt atccgctgca ttgcggtatt
     1321 ccatagccac gggccagagc tgcttgtagg tagaaatatt ctttatcagc accgccatat
     1381 ccgcaagccg gtgttccata gctttgaggg tagcggcggc ctcgtcgctg gcggcggtga
     1441 tctcggccac tttgctttcc aaatccgggt aggtgtctat cccatgttca gtcaggaaat
     1501 tcaaagtctt tgcggcctgc ttcaaattgt ggagcttcgc ccacctctca tagcctgccg
     1561 actgctgggc cttaatgttg ttttccagat caatccgcaa agaaatcccc ttgcgctctt
     1621 tgggcgctct ggtgataacg cggcctttga tacgctcttt gatggcttcc tcggtgtagg
     1681 cttcccccaa tgtcttgcag cgggtaaagc gttcctgtcc gggagcgcgg aaagaaataa
     1741 actttcccgg cttgatctca tagccctgcg cctgcatgag ccgcaggaaa tcatcaaaat
     1801 cttttgcctg cgggatagcg gcgtcaatgg ctgctttgag cttcgctttc cagctcgtcc
     1861 ctttccgctg ggcgtcccac tcggcatagc tcttgccctt actctggccg ggcttcacca
     1921 cggacaatcc gttttccttg cacagcctgt cgctggtgcg ccggatatag gcatagctct
     1981 gtttgttgga aatgtacttg cgctgattta ccatatccac ggcacagaaa ataatgtgat
     2041 tgtggacgtg gcccttatca atatgggtag tcagcacata gggatatttc ccttgcagca
     2101 cttcctcggc aagctgcttc ccgatctcat gggcctgttc cggcgtggct tcgccggggg
     2161 caaaggattg tatcaggtgg tggcccagat tattgccctt ttggtatgcc tgcgccagca
     2221 gcatttcaaa ttcaatgtcc gctgtttcat aagagcaggc aaaggaggcc accagcagct
     2281 tgccgtcagt tttgtccggg ttctcgatgt agtcaagggc ttttttcagc gttgacttga
     2341 taggatgtat ctttgtaact gccatatctg cgccagcgcc ccctttatat cctcgatgtc
     2401 ctgtgcgtac accggcccca taccgttcag cctctttgca atctgattga cgttgacgcc
     2461 gatcttctgt atctcggcgg tctgcgcccg gatgtcgctg gtgtccatgt tgacgatata
     2521 gccgtcaatg agcatcttcc gggcgtaggc tgaaaagttc ttcgtatgca gcagcgccat
     2581 tttctgttca atcatagccc gttcctccgg cgtcacaggg acgcgcagca cgatttttcg
     2641 ctttctgttt gccatgcttc accgtccttt ccattcaggg gtttggggat ttccccaaca
     2701 agcaaaacgg acgcggcccg gcagggacgg ggcaggccgt ttttccggga gtggctacac
     2761 tccctatgct tgctacgtta ctgtttctcc actaacaaga cgtttccaaa gccccaaact
     2821 acccgcaaaa tagccctctg gaaagaaaaa agtgcaaaaa aatagaagct acgctgtgca
     2881 gctcccgcta aaagtaaaac agaggatgcc agttacacat cctccgtttc attcttcttc
     2941 ggcttttcaa ttagtatggc gctgtcaatc gcgccctcga tgatcggcag gtattcttcg
     3001 gggcagagtt tcagcttgtg gctgaccctt tgccgcagtt cgctttcctc ccgcatgatc
     3061 tccgggttaa agtagcgttc cacaggaagc ccgcaggctt taataagctg tatgacaacc
     3121 ggcagactgg gaatggcccg tcgttttcga tgttggcaag atagcgccag tcaatattta
     3181 ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg
     3241 cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta
     3301 cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta
     3361 tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag
     3421 gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg
     3481 atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat
     3541 ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc
     3601 ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa
     3661 gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga
     3721 taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc
     3781 gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg
     3841 tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct
     3901 ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat
     3961 accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac
     4021 atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt
     4081 ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac
     4141 cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca
     4201 aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg
     4261 ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg
     4321 ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag
     4381 gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc
     4441 atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga
     4501 aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc
     4561 gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct
     4621 ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt
     4681 ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca
     4741 tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct
     4801 gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa
     4861 gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc
     4921 gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact
     4981 ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt
     5041 gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga
     5101 ctttgagggt gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc
     5161 ggaaaatatc atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg
     5221 gaaagaaaag ggattttcat taaggttttt tcctatacga tcattgtcct gctactgctt
     5281 gtcggtgtaa cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa
     5341 ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg
     5401 cttgatattc aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat
     5461 attgaagata aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt
     5521 tttagaccca actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa
     5581 agcaaggcag gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt
     5641 gcgataatgg ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg
     5701 ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg
     5761 ccgctggagc gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc
     5821 aggctgaaag aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag
     5881 gaaacacagc gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct
     5941 acaagcgttc tgttggaggg aatgcttgaa aatatcggtg actacaaaga tcattctaag
     6001 tatctgcgcg aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg
     6061 gagcttgtca gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg
     6121 cgcacggttg ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg
     6181 ttcgtcacag atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag
     6241 gcactatcca atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg
     6301 atatggagtg agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac
     6361 attgatgata ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcga
     6421 agcagaaaaa gtgggcgaag cagaaaaagt gggcgaagcg gtttaggact tgccatcgta
     6481 caaaaaacgc tggatgccat gagcctccag tatgcgctgg aaaacacctc ggatggcgtt
     6541 ttgttctggc tggatttacc gctcacatca acattgtaaa tatttaaaac ttaaatgatt
     6601 ttgggctctt tgtcaaatag gactgataga gtgagataag aacatctaag gtagaattcc
     6661 ttttctactt tagatgtttt ttgattcata ggtaatcaga ttatttagta agaaaattct
     6721 ggtcttcatt ttccgaaagc ttcgaaagcc ataagcgact cgtttcaatg ctttgatgtg
     6781 ggtatgcaga ttttctattt tggcatttga ataaggcaat tcgagggcta aacgaatgcc
     6841 ggattcatag tttataaaag ctttttttat agaatgcagt tcttttggaa cggaattggg
     6901 catcgttctt acaagtttaa agaaagtgtc cgcctctttg tcg
//
LOCUS       DAZAJZ010000263         5814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.137, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000263 DAZAJZ010000000
VERSION     DAZAJZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.137"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1027)
                     /locus_tag="ACG86D_003068"
     CDS             complement(<1..1027)
                     /locus_tag="ACG86D_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3142108.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAF"
     gene            1306..2112
                     /locus_tag="ACG86D_003069"
     CDS             1306..2112
                     /locus_tag="ACG86D_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN3142109.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2130..2837
                     /locus_tag="ACG86D_003070"
     CDS             2130..2837
                     /locus_tag="ACG86D_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN3142110.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            2834..3805
                     /locus_tag="ACG86D_003071"
     CDS             2834..3805
                     /locus_tag="ACG86D_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN3142111.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3798..4826
                     /gene="vanB"
                     /locus_tag="ACG86D_003072"
     CDS             3798..4826
                     /gene="vanB"
                     /locus_tag="ACG86D_003072"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN3142112.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            4832..5440
                     /locus_tag="ACG86D_003073"
     CDS             4832..5440
                     /locus_tag="ACG86D_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN3142113.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(5506..5679)
                     /locus_tag="ACG86D_003074"
     CDS             complement(5506..5679)
                     /locus_tag="ACG86D_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142114.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1650 a         1202 c         1468 g         1494 t
ORIGIN      
        1 gaaacgcttg aagaatttct tgatagtaaa aataggcgat tcgtaattcg tcagagatcg
       61 aaagcagacg atccaccact tcactttcgg tgagatacgc ccaaccaaaa ttgcgccatt
      121 tggtccattt gtcataaaca agcttgtcct gtcgtttgat cagaagccga gacaacgcct
      181 ttaattgtcg ataatatttg gcttgtgttg gatcgtttcg atcaaagctt ttcatgattc
      241 gtacgcgaat atcctcaaat ttacgaatga gatgtttgac aatgtgaaaa cgatccacaa
      301 cgagttttgc ctttggaaaa cattccttta tcagagaggc ataagaaaca ttcatgtccg
      361 tcacgatgac ctttaccttc aatcgtgcgc gtcgttcaaa ttgcataaaa taagaaacga
      421 gagccttttt cttccgtgaa ggaagaatat ccagtaattt tccagtatcg ccatccatac
      481 aagaaaagga gtagggaccg acttgatttt tcagtgaacg aaattcatcc attaataaac
      541 aacgcggtaa ccaatttctt tttacttttg tcatggggat cagagaagcg agtgtccgag
      601 aaacggtcga ccaagaaacg ccgcaatgct tagcaattgt cgtcattgct tgtttttcag
      661 ttagctcttc taaaatttta tagcgcactt gtttagcgat aaagcagttt ctatcgacaa
      721 tgtaggtatt cgccgtaaaa taggtgtggc agccacgaca gtggtagcgt tgtttcgcta
      781 aagcaagata agtcagttgg ttcgcacatt tattaagttg aatgagcgat tttttctttc
      841 cgttcttaac gatcatgcct tcatgattca tctgtttaca caaaggacag cgacgaattt
      901 ctttacttaa cgtcgcagaa acaacaagac tcttgatatt cttaatgctc ttcgattcca
      961 catttgtgat gaaaatgttt ttatctttga ttccaagtag tagtttgata tgatgaagat
     1021 caggcataca gaacgctcct ttgattgtgt tgtggtgatt caattctaac ggagcttctg
     1081 tatgttttgt tttttcatgc tcaaaaggga ccgatagaat gattttctat cagtcctaga
     1141 aattatagag ccgattttga ccgacaggta taaccctgcc ggtctttttt tgctgctaca
     1201 ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat aacaatcgta
     1261 ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga aaaaagcaac
     1321 tatcattcca atgcggatca tcacaaacgg catatgaaac aatccgtgga aaagcgggct
     1381 tttctatgtg cgttcattat ttcgttcaca gtctgcacgc tgtttttggg gtggagactg
     1441 gcttccgtat tggaggcaac acagataccg cccatccctg caactcatac aggcagcagc
     1501 actgacgtag tggagaattt ggaggaaaac gctcttgcca ccgccaaaga acagggagat
     1561 gaacaggaat ggagcctgat tttagtgaac aggcagaacc ccatccccgc acagtacgat
     1621 gtggagcttg agcaactatc aaatggtgag cggatagata ttcggatttc tccctatctt
     1681 caagatttgt ttgatgccgc aagaactgat ggagtttacc cgattgtcgc atccggatac
     1741 cgaacaacag aaaaacagca agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa
     1801 ggctacacct ctgcacaggc taaagcggaa gcagaaactt gggtggccgt gccgggaacg
     1861 agcgagcatc agcttggtct tgctgtggat atcaatgcgg acggaattca ttcaacaggc
     1921 aacgaggttt atagatggct ggatgaaaac agctatcgtt ttggttttat tcgtcgctac
     1981 ccaccagaca agacagagat aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt
     2041 ggcatcgaag ctgccacaga gatgtacaac caaggggttt gccttgagga atatttaaaa
     2101 ccagaaaaat gagaaaagga tgttatgtta tggacagaaa aagattgaca cagcgtttcc
     2161 cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg ggaatgagat
     2221 ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat ttgctctttg
     2281 aaacggattg cgcattatac aaccacaata ccggatttga catgatatac caggaaaaca
     2341 aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata agaccggggg
     2401 aaaccttttc tttctggttg ttcctgcata cgccattgac aattatccag cgcagaggtc
     2461 acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg gatgcaacca
     2521 tttcagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc taccaaatat
     2581 gggtgacctt agatgatgag aaaatcatcg gtcaggtgtt cgccgacaaa gagccccaag
     2641 cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc gggatttatg
     2701 aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata atagattgca
     2761 agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt gtggatattc
     2821 aggaggagaa ccaatgagaa aaagtgtggg cattactgtt tttggatgcg aacaggatga
     2881 ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt atccctacgc tgattagcga
     2941 tgcgatatcg gcagacaacg caaaattggc cgctggcaat caatgcgtta gcgtaagcca
     3001 taagtccgag gtttccgagg cgacaattct tgcgctgaga aaggtcgggg taaaatacat
     3061 ttctacccgc agcatcggct gcgatcacat tgatacgact gccgccgaga gaatgggaat
     3121 ctcggttggc acggttgcgt attcgccgga cagcgttgcg gattatgctt tgatgctgat
     3181 gctgatggcc atacggggtg caaaacccac catgcacgcc gtggcgcaac aagatttcag
     3241 attggatcgt atccggggga aagaactggg ggatatgact gtgggagtta ttggaaccgg
     3301 ccatatcggg caagcggtcg tcaaaaggct gcggggattt ggatgccatg tgctggccta
     3361 tgataacagc cgaaaaatgg atgcagatta tgtccagctt gatgagcttc taaaaaacag
     3421 cgatattgtt acgctccatg tgccgctttg tgcggatacc cgccatctga tcggtcagaa
     3481 gcaaattgga gagatgaagc aaggcgcatt tttaatcaac accgggcgcg gagcgcttgt
     3541 cgataccggg gcgctggtgg aggcgttgga aagcggaaag ctgggtggtg cggcactgga
     3601 tgtgttggag ggcgaggatc agtttgttta taccgattgc tcgcagaaag tgcttgacca
     3661 tccctttttg tcgcagcttt taaggatgcc gaatgtgatc atcacacccc atacggcgta
     3721 ctacactgag cgtgtgctgc aggatactac agaaaaaaca atcaggaatt gtctcaattt
     3781 tgaaaggagt ttacagcatg aatagaataa aagtcgcaat catcttcggc ggttgctcgg
     3841 aggaacatga tgtgtcggta aaagccgcaa tagaaattgc tgcgaacatt gatacggaaa
     3901 aattcgatcc gcactacatc ggaattacaa aaaacggtgt atggaagcta tgcaagaagc
     3961 catgtacgga atgggaagcc gacagtctcc ccgccatact ctccccggat aggaaaacgc
     4021 atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg gcgtattgat gtggctttcc
     4081 cggttttgca tggcaaatgc ggggaggatg gtgcgataca ggggctgttt gtattgtctg
     4141 gtatccccta tgtgggctgt gatattcaaa gctccgcagc ttgcatggac aaatcactgg
     4201 cctacattct tacaaaaaat gcgggcatcg ccgttcccga atttcaaatg attgataaag
     4261 gtgacaagcc ggaggcgggt gcgcttacct accctgtctt tgtgaagccg gcacggtcag
     4321 gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga acttaacgct gcgatagaag
     4381 cggcaggaca atatgatgga aaaatcttaa ttgagcaagc gatttcgggc tgtgaggtcg
     4441 ggtgtgcggt catggggaac gaggatgatt tgattgtcgg cgaagtggat caaatccggc
     4501 tgagccacgg tatcttccgc atccatcagg aaaacgagcc ggaaaaaggc tcagaaaatg
     4561 cgatgattac agttcccgca gacattccgg tcgaggaacg aaatcgggtg caggaaacgg
     4621 caaagaaagt atatcgggtg cttggatgca gagggcttgc ccgtgttgat ctttttttgc
     4681 aggaggatgg cggcatcgtt ctaaatgagg tcaataccct gcccggtttt acatcgtaca
     4741 gccgctaccc acgtatgatg gccgccgcag gaatcacgct tcctgcactg attgacagcc
     4801 tgattacatt ggcgttaaag aggtgataag tatggaaaat ggttttttgt ttttagatga
     4861 aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca tgggataact tcacaggaaa
     4921 accggtggat gggtatgagg tgaatcgcat catcggcaca aaggccgtgg cgtttgctct
     4981 gcgcgaggca caaatccatg cggctgcgct tggctatggc ttgcttttat gggatggata
     5041 tcggccaaga actgcggtgg actgcttcct gcgttgggca gcgcaaccgg aggacaatct
     5101 cacaaaagaa aaattttacc ccaatataga gcgagccgag ttgattacaa agggttatgt
     5161 ggcctcacaa tccagccata gccgtggaag cgcaattgat cttacgctct accacctgga
     5221 tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt atggacgaac ggtcgcatca
     5281 tacagcaaaa gggatagggg atgcagaggc acaaaatcga agatgcttgc gtaaaatcat
     5341 ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg cactataagt tgattgatga
     5401 gccatacccc gatacctatt ttaattttgc cgtttcataa tgaaagtatt ggttttgtat
     5461 cagattttgg atttgataaa atctatgctg gtcatggaca atcttttact ggaaagtggt
     5521 taaagcatct tttgtaatac attgcaaatt atagtattca atctgcccag cggcagaaaa
     5581 gaaaaaaacc gctgttgggc agacctttcc cacgcccatt ttgtattttg ctggcggcgg
     5641 ttttgagaaa tcaaggccgc cgttcttttc tggcagcaaa aggtgtggcg gcccatagga
     5701 ggatgccgct atgctgtttt gctattgcca tgctctaatc cacttccacc actcaaaaaa
     5761 agccttgccc ctccatgaac tgaaccccaa aagttgaact atttaatgga ctgt
//
LOCUS       DAZAJZ010000264         3935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.161, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000264 DAZAJZ010000000
VERSION     DAZAJZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.161"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..674
                     /locus_tag="ACG86D_003075"
     CDS             39..674
                     /locus_tag="ACG86D_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3142115.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            810..902
                     /locus_tag="ACG86D_003076"
                     /pseudo
     CDS             810..902
                     /locus_tag="ACG86D_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1016..1651
                     /locus_tag="ACG86D_003077"
     CDS             1016..1651
                     /locus_tag="ACG86D_003077"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3142116.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            1758..2996
                     /gene="mef(H)"
                     /locus_tag="ACG86D_003078"
                     /gene_synonym="mefH"
     CDS             1758..2996
                     /gene="mef(H)"
                     /locus_tag="ACG86D_003078"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN3142117.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            3223..3642
                     /locus_tag="ACG86D_003079"
     CDS             3223..3642
                     /locus_tag="ACG86D_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN3142118.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            3647..3886
                     /locus_tag="ACG86D_003080"
     CDS             3647..3886
                     /locus_tag="ACG86D_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3142119.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         1355 a          551 c          758 g         1271 t
ORIGIN      
        1 agcatgatag acttataata gcgaagatat aacgagatat ggagggttat gcaatgaaaa
       61 taagccattt aggaaataat atacagacca taagaaaatt taggggaatg aaacaacagg
      121 aacttgcgga caaaatcggt atcaatatgc agagcctttc caagattgaa agagggttga
      181 actatcctgc ctatgaaacg ctggaaaaga taatggaagt gctggacgtt acgcccaatg
      241 aattgttgtc gggagaatgg aagtatgtca atcaatctga aaaggaagtc tgtcagtttt
      301 taagagccga agaacgtcta aatgcagaac tgaaacatgg acactatgat aacttctttg
      361 acagcgagga agaatggctg gaatatgagt tagaaaagtt acgggaatat attacagact
      421 atatcaacgg gaaaagcatt gaagcgtccg acctttaccc aatcaaagaa tttatccagc
      481 atttgaagtt tcaaaaatta ttagaccgct atgatgattt atacagtatg gatatgtttg
      541 gggaaagcat agagggacat aagtataaga caccttatca agtcgtgaaa atgataaacc
      601 cgaactcaaa agaagatatg gaactgttac gagaagtatt aaggaataac aattttgatg
      661 atgaggacga gtaacttttt tcctcttgcg agaagataca gcaaacaaaa agcccgatca
      721 agagtgcaaa gcaccaccaa tggtggcaaa gctcttgaca gggctttttc ttttctgttg
      781 tgggcaatca agaggaagaa agggataaaa tctgatttgt ggtaaatcag gcaaaggcaa
      841 cgcaggtatc acagtatgaa ttgacaccgc ataaggaaag caactggaag attgtagaat
      901 aaatgaaaaa ggcttgcatt ctgcattttt ataaaattgc aagtctttat attgacaagc
      961 ataaaatact aagatatact ttaatcagac caacggtttg tattggaggt aagttatggc
     1021 acgaaataaa tatccagaag aaacagtaaa actcatttta gatgtttcta caaaaatgtt
     1081 ttgtgaaaaa ggatatgata acacatcatt gcaagatatt atcaatgaaa caaaactttc
     1141 taaaggtgct atttatcatc atttcgattc aaaggaagat attttaaaag caatttttca
     1201 aagaattgga aatgaaaatg cagtaatatt tgctaaaatt agagataacg atagtttaaa
     1261 cggtttagaa aagttaagag aaatatttaa gacagcggta ttcaattcta accaaagcgt
     1321 tttattgaca gtttctcctc aactattaaa caatcctcgt tttcttgcta tgcaaataga
     1381 acaaatgtat gaaattgtag tacctgaatt tgttgaaccc gttttagaac aaggtatcaa
     1441 ggataagtct ttggagattg aaaattcaca tgaaattgca gaagttatca tggtattaag
     1501 taatgtatgg ctcaacccat tagttgaaat gacggataaa gagggtatgg aaaaacgttg
     1561 caatacattc aatgcccttt taaatggaat aggcatagac acattattag acgaagaaac
     1621 aatttctgca tttatacagt tttgtaaata actgtatttg ttatccctat acgatagact
     1681 gctctataaa agagcagttt atttaaacca ttatataaac cgacggtctg tttgattaaa
     1741 taaaaaggag agattttatg caagcagaaa aaaaactttt taatcgtgat tttatacttg
     1801 ttgttatagg acagataatt tctttatttg gaaacgctat cctacgattt gcattaccgt
     1861 actatttgtt aaaagaaact ggttcatcag ctttgtttgg tattgctaca gcctgctctt
     1921 ttttacctat ggttattttg tcgctggtag gtggtattat tgctgacagg acaaataaaa
     1981 gaaacattat ggtcatacta gattttgtaa cagcaatcat aattttagta ttttatttgt
     2041 cattgggaaa aattcctatc atacctttgt ttattgttgc tttgatgtta ctttatggta
     2101 tttctggtgc atatcaacca gcggtacaag caagtattcc tctattggta tcatcagata
     2161 aacttaccgt cggtaatgct ataataaatc aagtaaatac tttagcaaac tttatcggac
     2221 cagctataag cggagtagtt tttcaatttt atggtatcaa tcccatttta attgttagta
     2281 ttatatgttt tactttttca gccattatgg aaatttttat ccatattcca caccaaaaaa
     2341 gacctcgtga agtaggtgta attgcagttg ctaaaaacga tttaaaagat agttatacct
     2401 ttttgaaaaa ggagaaaccc atttttatat ccgtaatctt tttggtttgc attttcaatt
     2461 tggttttaag tgctgtttta attgttggtc tgcctatctt aatttctgaa gttttaaaaa
     2521 tgccagatac actatatggt ttttcacaat cagcattatc attaggtggt ttatgtggtg
     2581 gtattttaac agctcttgtt gcttcaaagt taaaactaaa aaacctttat accttgttac
     2641 ttatttgttc tgtttctgtt ttacttatgg gaatttcgct atttttagaa ttgcctgcaa
     2701 tagtttccta ctgggtagtt acagtgatgt gctttgtggc tatgggtgta tctactatgt
     2761 ttgttgttca aatttacact cttgttcaga cacaaacacc accgcaatta gtaggaaaga
     2821 taatggctac gctggtagca gttgctatgt gcggtcaacc tattggacaa atggtttacg
     2881 gtattttatt cgatgtattt atacaagaaa catgggttgt tatgtttgta gcttctattg
     2941 cttcaatcat gatttcaata tattcaaaaa gaatttttca aaagttagag atataataca
     3001 aggggttaaa tagtatgtta ggctaaaaac aatatataaa agaaaatttg aaatctgcta
     3061 aaaggatagt gttataaaag aaagagtaat attgcagtat ttaaggctcg cacaaatata
     3121 tgttttgtgt gggctttttt cttaccattt ttctgtattt ctaaagggta taaacggtat
     3181 tccgtttata tatttcagca ttagcagaaa gggggtgaga ttatgaggac atcttctttc
     3241 gagcatatcg tcagattaca gttcaattct ttaatgctga ttgtcattaa gaacaaagtg
     3301 aaaagtcgaa acagacagct tgcaagacat tctaaacgtg aagtattgtt ttgtgaaata
     3361 gccgaaacga aacaagttga acgtggaacg attgatacct gtttttatga ttgtgtatca
     3421 tttaaagtct tgcactttac aatttatgta tctgatgaaa cacttggtac agttttacat
     3481 agactgtcag aaaaacagcg tagtgcgatt ttattacgtt attttcaagg aatgaacgac
     3541 agagaaattt cagaactgta ccatgtatca cgttctgcta tttgcagtag aagaactaga
     3601 ggactaagga agttaaaaat gcttttgagc gaaaggaagt aaagaaatga gtaaagatta
     3661 tggctatccc tcttttgaat tgatttgcaa agcgtcaagc ggagatgaaa tggcagttaa
     3721 agagattttg aagttttatg acggttatat ttctaaatta tgcttgcgtc ctttttacca
     3781 ctctgaaagt ggtaaaatca ttatgcaggt tgatgaagaa ctaaaaggag aaattcatac
     3841 agacatgatg aaagcaatat tgaaatttga aatcggagtg aagtaatcaa ctgtataaaa
     3901 aaatcgggag atacacagtt tcgtgctatc tccct
//
LOCUS       DAZAJZ010000265         3796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000265 DAZAJZ010000000
VERSION     DAZAJZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.164"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACG86D_003081"
     CDS             110..193
                     /locus_tag="ACG86D_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN3142120.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG86D_003082"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG86D_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN3142121.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACG86D_003083"
     CDS             complement(1318..2181)
                     /locus_tag="ACG86D_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3142122.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACG86D_003084"
     CDS             complement(2183..2455)
                     /locus_tag="ACG86D_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3142123.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACG86D_003085"
     CDS             complement(2472..2681)
                     /locus_tag="ACG86D_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN3142124.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACG86D_003086"
     CDS             complement(2759..3655)
                     /locus_tag="ACG86D_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3142125.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DAZAJZ010000266         2256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.180, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000266 DAZAJZ010000000
VERSION     DAZAJZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.180"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1014
                     /locus_tag="ACG86D_003087"
     CDS             322..1014
                     /locus_tag="ACG86D_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3142126.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG86D_003088"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG86D_003088"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN3142127.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..>2256)
                     /locus_tag="ACG86D_003089"
     CDS             complement(1910..>2256)
                     /locus_tag="ACG86D_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN3142128.1"
                     /translation="YSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLE
                     RPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITD
                     KQFDWKDIIYFK"
BASE COUNT          892 a          296 c          404 g          664 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagctggaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaa
//
LOCUS       DAZAJZ010000267         1415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.203, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000267 DAZAJZ010000000
VERSION     DAZAJZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.203"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..304)
                     /locus_tag="ACG86D_003090"
     CDS             complement(<1..304)
                     /locus_tag="ACG86D_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN3142129.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            397..746
                     /locus_tag="ACG86D_003091"
                     /pseudo
     CDS             397..746
                     /locus_tag="ACG86D_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1018..1308)
                     /locus_tag="ACG86D_003092"
     CDS             complement(1018..1308)
                     /locus_tag="ACG86D_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3142130.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          448 a          281 c          191 g          495 t
ORIGIN      
        1 caacatactt gcaaagtaat actacaattc ctaacataat cgtagaattt aataatttcc
       61 atagcataaa gtgtttagaa aagaacatta agaatagttc tataataatt cttgaactcc
      121 atgtcttata atgttctatc gaagcaccca aatagccaag ttcatctaat ctacttgcat
      181 aaactacgtc atccgctctc atagtcggca tatacatatt aaagagtaaa aagatacccc
      241 aaatccctat tagtaacaag atcagttctt tgttattcgt tatttttcta ttatgtttgt
      301 tcatttttat actccttctt atatttccta atagagtata acatagcttt aagcattact
      361 taaagataac ttaaaagtta tcttttaaat acttaattgt taaatacctt atttgatgca
      421 aaagaaatct acgatagtca acaaaaaaat tttcattata tccagaaaaa tttttcttag
      481 ttaaggacct atggattgca gtaatgcaga attcgtcgaa taaactgcca aagacataca
      541 atcatattgc ttttaaaatt gacgaacaag atatagattc gtttgtctca aaaatccaaa
      601 tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt
      661 acttttacga ctatgataat cacctcttcg agttacatac cggtactctc caggaacgtt
      721 taaaatccta caattccact acctgagact agtaatgact agtctctttt tatggcctct
      781 gatcccccca ttgagcgaat actaataggg cttcgaattg atctgttcaa tctttaagca
      841 atatccctca aatgagtttg gaaagtttag tgattcatca caaatactta aatacatgta
      901 gttatatata gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc
      961 tgcatccatc tttccaaaac aagcggctag gacatttttg tctcagccgc ttgttttcta
     1021 gtttattctt tcatcttgtc ggaccttttt tcttgggtat agacttttct catattctac
     1081 cacaattttg ggtttcccta acacatgata ggatctcaca acaaattgct ttctattatt
     1141 ataataacct gtaacgacta acttcatccc ttcgtctact tcagctagga agttcaaact
     1201 atgggaagca atcaaacaat tgaccccatt taaactgaaa cggatcaacg gtctggcact
     1261 aacttgcaaa gtacggatct tatctactgt tccatatttt ttctccataa ccttcacctc
     1321 aataccatta tacgaacata cattcccttt gtaaagaaga aactctgttt tatctttttg
     1381 actttttgtc tattaccttt cttaaaatca tattg
//
LOCUS       DAZAJZ010000268          945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6619
            SAMEA115708484-rid22583323.guided.219, whole genome shotgun
            sequence.
ACCESSION   DAZAJZ010000268 DAZAJZ010000000
VERSION     DAZAJZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708484
            Sequence Read Archive: ERR13198206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288415.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288415.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:38:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 29 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708484-rid22583323.guided.219"
                     /isolate="VRE6619"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            332..880
                     /gene="aac(6')"
                     /locus_tag="ACG86D_003093"
     CDS             332..880
                     /gene="aac(6')"
                     /locus_tag="ACG86D_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN3142131.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          337 a          135 c          190 g          283 t
ORIGIN      
        1 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
       61 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
      121 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
      181 aataaagtgt ttctgtataa ttttttattt tataagtaag ataaataatg aaagaataga
      241 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
      301 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
      361 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
      421 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
      481 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
      541 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
      601 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
      661 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
      721 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
      781 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
      841 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
      901 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tactt
//
